Milad Kheirvari, MSc - Pasteur Institute of Iran - Assistance researcher

Milad Kheirvari


Pasteur Institute of Iran

Assistance researcher

Tehran, Tehran | Iran (Islamic Republic of)

Main Specialties: Biochemical Genetics, Biology, Biotechnology, Clinical Neurophysiology, Forensic Psychiatry, Infectious Disease, Medical Microbiology, Medical Toxicology

Additional Specialties: Biochemistry

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Milad Kheirvari, MSc - Pasteur Institute of Iran - Assistance researcher

Milad Kheirvari



Milad Kheirvari is a biochemist, initiating his studies in physiological researches on Cervical Cancer. He continued his studies on the ultrasound effects on the body. In 2015, he began his MSc dissertation on the recombinant vaccine of UTI. He went on to the study of microbial contamination in donated tissues such as Tendon and Corneal in forensic medicine of Iran. He also has explored various polymorphisms in post-chemotherapy toxicity in colorectal cancer patients. He's also studying on the effects of Laparoscopic Sleeve surgery on Gut Microbiota and Bulimia Nervosa.

Primary Affiliation: Pasteur Institute of Iran - Tehran, Tehran , Iran (Islamic Republic of)


Additional Specialties:

Research Interests:

View Milad Kheirvari’s Resume / CV


Jan 2014 - Jan 2016
Islamic Azad University Pharmaceutical Sciences Branch
Jan 2010 - Jan 2014
Islamic Azad University Varamin-Pishva Branch

Jan 2015
Pasteur Institute of Iran
molecular biology




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Socio-demographic Characteristics, Biochemical and Cytokine Levels in Bulimia Nervosa Candidates for Sleeve Gastrectomy.

Arch Iran Med 2020 01 1;23(1):23-30. Epub 2020 Jan 1.

Department of Molecular Biology, Pasteur Institute of Iran, Pasteur Ave., Tehran, Iran.

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January 2020
1.108 Impact Factor

Socio-demographic Characteristics, Biochemical and Cytokine Levels in Bulimia Nervosa Candidates for Sleeve Gastrectomy

Arch Iran Med. 2020;23(1): 23-30.

Archive of Iranian Medicine


Eating disorders (EDs) are widely known by abnormal eating behaviors associated with significant medical complications. Bulimia nervosa (BN) is an eating disorder characterized by uncontrolled episodes of overeating typically followed by some form of compensatory behaviors. We aimed to determine the relationships between socio-demographic characteristics, biochemical markers, and cytokine levels in BN candidates for laparoscopic sleeve gastrectomy (LSG).


A case-control study was designed among 76 BN participants of Iranian descent who were candidates for LSG based on defined criteria for Bulimia by Diagnostic and Statistical Manual of Mental Disorders (DSM-IV). The healthy control subjects (n = 42) were selected at random from academic staff in the college. Moreover, levels of biochemical parameters and serum cytokines were measured in serum samples.


Routine consumption of caffeine (odds ratio [OR] = 3.1, 95% CI: 1.23-6.41, P = 0.013), tobacco (OR = 1.8, 95% CI: 0.67-3.57, P = 0.03), and alcohol (OR = 3.6, 95% CI: 0.84-7.18, P = 0.048), and depression history (OR = 2.8, 95% CI: 0.76- 5.79, P = 0.037) were substantially more common among patients with bulimia. Also, the serum levels of fasting blood sugar (P < 0.001), HbA1c (P = 0.04), cholesterol (P = 0.03), triglycerides (P = 0.01), blood urea nitrogen (P = 0.03), and pro-inflammatory cytokines including IL-1β, IL-6, and TNF-α were significantly higher in BN candidates for LSG (P ≤ 0.001).


Our findings reveal that lifestyle-related risk factors and a depression history were both related with a significantly increased risk of BN among the candidates for LSG. Furthermore, there is a relationship between clinical characteristics as well as levels of various biochemical and cytokines parameters in serum of BN patients.

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January 2020

Impact Factor 1.000

Prevalence of Self-Medication among Population of Tehran, Iran

Asia Pacific Journal of Medical Toxicology, 2019; 8(1): 14-18.

Asia Pacific Journal of Medical Toxicology

Abstract Background: Medications nowadays have been commonly used items in the world and are sometimes available without prescription. This causes a range of effects on public health. This study evaluated the frequency and the involved factors of uncontrolled prescription drugs consumption. Methods: The present study is a cross-sectional study on 1,000 citizens of Tehran between 2016 and 2017. The various factors such as age, gender, and educational background have been carefully surveyed. The questionnaires were presented to interested people in printed form. For analyzing the data, descriptive statistics and analytical statistics were performed using SPSS software version 19. Results: 67% of participants use OTC drugs and 34% of them were between 20 and 30 years old. Although 12% of the OTC pills were used regularly as a pain killer, 95% of the people did not know anything about the side effects of the drugs. Moreover, 45% used the pill to relieve the common and acute pain and 34% of people lived in poverty and 12% suggested lack of adequate time as a reason to refuse visiting a doctor. Conclusion: Self-administered drug is one of the problems in the medical field. Headache is the main reason of self-medication. In the other hand, drug resistance is also rising among repeated drug users. According to the results, public education, the prohibition of the sale of some high-risk drugs and the reduction of drug doses are ways to prevent the development of such high-risk habits.   Keywords Humans; Iran; OTC Drugs

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May 2019
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Genotypic identification of Pseudomonas aeruginosa strains isolated from patients with urinary tract infection

Comparative Immunology, Microbiology and Infectious Diseases Volume 65, August 2019, Pages 23-28

Comparative Immunology, Microbiology and Infectious Diseases


Numerous nosocomial infections including urinary tract infection (UTI) have been reported to be linked to Pseudomonas aeruginosa (P. aeruginosa). This bacterium is one of the most common pathogen colonized in the urinary tract. The main purpose of this study was to evaluated the presence of antibiotic resistance genes and also the most frequent genotype patterns of P. aeruginosa in the patients with UTI hospitalized in different wards of hospitals.

Materials and methods

In this study, 70 strains of P. aeruginosa isolated of urine samples from the patients with UTI were assessed. The isolated strains were genotyped using Multiple-Locus Variable Number Tandem Repeat Analysis (MLVA) method. We have also analyzed the presence of TEM and SHV resistant genes in the isolates.


A total of 70 P. aeruginosa strains was isolated from the UTI patients. Based on MLVA method, 61 various genotypes of P. aeruginosa were identified which grouped into two main clusters and 4 sub-clusters. Moreover, approximately 80% and 70% of isolated strains carried the TEM and SHV resistance genes, respectively.


Our findings showed that the majority of patients hospitalized in different wards of hospitals have experienced the urinary tract infection caused by P. aeruginosa. According to the genotyping results, a high diversity of the P. aeruginosa population was observed in the patients with UTI. Our results can provide a better understanding of the P. aeruginosa genotype distribution and epidemiology of infection, which can be applied as basic data for future antibiotic therapies.

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March 2019

Impact Factor 2.000

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Changes in Gut Microbiota and Hormones After Bariatric Surgery: a Bench-to-Bedside Review

Tabasi, M., Ashrafian, F., Khezerloo, J.K. et al. OBES SURG (2019).

Obesity surgery

Overweight and obesity are among the most prevalent non-communicable diseases which are generally treated successfully by bariatric or sleeve surgery. There are evidences affirming that sleeve surgery can manipulate the pH of the stomach and interact with the metabolism of fatty acids, carbohydrates, and bile acid transfer, leading to the overgrowth of gut microbiota. Therefore, this study aims to review the changes in gut microbiota and hormones after bariatric surgery.

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February 2019

Impact Factor 4.000

3 Reads

Specification of Bacteriophage Isolated Against Clinical Methicillin-Resistant Staphylococcus aureus

Osong Public Health Res Perspect. 2019 Feb; 10(1): 20–24.

Osong Public Health and Research Perspectives


The emergence of resistant bacteria is being increasingly reported around the world, potentially threatening millions of lives. Amongst resistant bacteria, methicillin-resistant Staphylococcus aureus (MRSA) is the most challenging to treat. This is due to emergent MRSA strains and less effective traditional antibiotic therapies to Staphylococcal infections. The use of bacteriophages (phages) against MRSA is a new, potential alternate therapy. In this study, morphology, genetic and protein structure of lytic phages against MRSA have been analysed.


Isolation of livestock and sewage bacteriophages were performed using 0.4 μm membrane filters. Plaque assays were used to determine phage quantification by double layer agar method. Pure plaques were then amplified for further characterization. Sulfate-polyacrylamide gel electrophoresis and random amplification of polymorphic DNA were run for protein evaluation, and genotyping respectively. Transmission electron microscope was also used to detect the structure and taxonomic classification of phage visually.


Head and tail morphology of bacteriophages against MRSA were identified by transmission electron microscopy and assigned to the Siphoviridae family and the Caudovirales order.


Bacteriophages are the most abundant microorganism on Earth and coexist with the bacterial population. They can destroy bacterial cells successfully and effectively. They cannot enter mammalian cells which saves the eukaryotic cells from lytic phage activity. In conclusion, phage therapy may have many potential applications in microbiology and human medicine with no side effect on eukaryotic cells.

Keywords: bacteriophage, methicillin-resistant Staphylococcus aureus, random amplified polymorphic deoxyribonucleic acid - polymerase chain reaction, scanning transmission electron microscopy, sodium dodecyl sulfate-polyacrylamide gel electrophoresis

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January 2019
5 Reads

The Effects of Oxymorphone on Biochemical Parameters of Male Rats

Journal of Clinical and Diagnostic Research

Abstract: Introduction: Oxymorphone is similar in structure to morphine. It is used for the relief of pain that was approved by the Food and Drug Administration (FDA) in 2006, which may have side effects on few parts of the body. Aim: To illustrate the changes in biochemical parameters in response to the injection of oxymorphone in adult male rats. Materials and Methods: This research was carried out on 50 male adult Wistar rats which were divided into 5 groups that included 2, 4 and 6 mg/kg of Oxymorphone received groups, control group and normal saline received group. Injections were administered intraperitoneally via a catheter once a week for 56 days. After eight weeks, blood samples were collected using cardiac puncture method. Following serum preparation, enzymes and hormones levels were quantified using standard automated spectrophotometer especially photometric kinetic methods. All values were presented as mean±SEM. Statistical significance was evaluated by one-way analysis of variance (ANOVA, t-test) using IBM SPSS Statistics 20.0 for Windows. Results: The results demonstrate that serum levels of Adrenocorticotropic Hormone (ACTH), cortisol, aldosterone and Thyroid-Stimulating Hormone (TSH) increased in all groups which received oxymorphone. The serum levels of Creatine Kinase (CK) had significant decrease in all exposed groups. Oxymorphone had no statistically significant impact on the serum levels of Alkaline Phosphatase (ALP). Serum levels of Alanine Aminotransferase (ALT), Aspartate Aminotransferase (AST), T3 and T4 had a significant change in group which received 6 mg/kg of oxymorphone. No definite changes in other groups. Conclusion: Exposure to 2, 4 and 6 mg/kg of oxymorphone may lead to short and long-term side effects. These effects can be therapeutic effects for a range of patients and can also be very deadly for a group of patients with unbalanced serum levels of special enzymes or hormones.

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September 2018
6 Reads

Epidemiology of Occupational Accidents in Semnan Workers Referred to Legal Medicine Organization Center of Semnan

Iranian Journal of Forensic Medicine

Background: Occupational incidents are among the inevitable threats that the working community has always faced and, in most cases, fear of being dismissed, and the agreement with the employer, leads the injured person to give legal prosecutions and the rights of the individual up. Issues related to this topic are discussed in this research project.
Materials and Methods: In the cases of occupational injuries that have referred to legal medicine centers from 2015 to 2017, information about characteristic of damaged or dead persons have been recorded in separate information forms and were analyzed with SPSS19.
Findings: The majority of the participants of this study were male. Those with the age of 26-30 years old (34%) were also the majority. The most injured persons were under diploma. 25% of them had complained from one week to one month after injury, but about 6% had complained over 3 years after injury. 28% did not have an occupational injury report. 35% were fired. 45% experienced degrees of permanent disability of which the most was in upper limbs. In about 31% of dead persons, brain and skull injury were the cause of death.
Conclusion: Both the workers and their employers must be well-aware about their rights. In case of an incident, it should be reported immediately, and the injured should be able to pursue legal actions without fear of the employer, and preferably with the direct encouragement of the employer. Forensic medicine must also ensure the correctness and accuracy of pre-employment examinations.

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March 2018
3 Reads

Assessment of biochemical parameters after exposure to ultrasound waves; an in vivo study

Biomedical Research


The main aim of this study was to determine the effects of ultrasonic waves on serum levels of lactate dehydrogenase, aspartate transaminase, alanine transaminase, creatine phosphokinase and alkaline phosphatase in male rats. Normally, Serum level of LDH, AST, ALT, CK and ALP enzymes are influenced by environmental factors. These enzymes commonly measured as biomarkers for life health.


In this study male wistar rats were randomly divided into two groups, control and exposed to ultrasonic waves for 1, 6 h/day. Blood samples were obtained using cardiac puncture method. Following serum preparation, biochemical levels were measured. Data were statistically analysed and compared between groups using ANOVA and student t-test.


The results indicated that serum level of ALT and LDH has a significant decrease. No differences were seen in the level of the serum of AST, ALP and CK enzymes in both the 1 h/day and 6 h/day of exposure group. And also serum level of ALT that has exposed 1 h/day comparing to control group didn’t have significant changes.


Exposure to ultrasonic waves may lead to side effects. These effects can be therapeutic effects for a range of patients and can also be very deadly for a group of patients with unbalanced serum levels of special enzymes.

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July 2017
5 Reads