Publications by authors named "Jacek Zieliński"

76 Publications

Non-invasive in vivo human model of post ischaemic skin preconditioning by measurement of flow-mediated 460-nm autofluorescence.

Br J Clin Pharmacol 2021 Mar 31. Epub 2021 Mar 31.

Department of Cardiology Intensive Care Therapy and Internal Medicine, Poznan University of Medical Sciences, Poznan, Poland.

Aims: Transient ischaemia and reperfusion (TIAR) induce early ischaemic preconditioning (IPC) in different tissues and organs, including the skin. IPC protects tissues by modifying the mitochondrial function and decreasing the amount of the reduced form of nicotinamide adenine dinucleotide (NADH). Skin 460-nm autofluorescence is proportional to the NADH content and can be non-invasively measured during TIAR. We propose a non-invasive in vivo human model of skin IPC for studying the effects of repeated TIARs on the NADH content.

Methods: Fifty-one apparently healthy volunteers (36 women) underwent three 100-second forearm ischaemia episodes induced by inflation of brachial pressure cuff to the pressure of 60 mmHg above systolic blood pressure, followed by 500-second long reperfusion episodes. Changes in skin NADH content were measured using 460-nm fluorescence before and during each of the three TIARs.

Results: First two TIARs caused a significant reduction in the skin NADH content before (p=0.0065) and during the third ischaemia (p=0.0011) and reperfusion (p=0.0003) up to 3.0%. During the third, the increase in skin NADH was 20% lower than during the first ischaemia (p=0.0474).

Conclusions: The measurement of the 460-nm fluorescence during repeated TIARs allows for a non-invasive in vivo investigation of human skin IPC. Although IPC reduces the overall NADH skin content, the most noticeable NADH reduction appears during ischaemia after earlier TIARs. Studying the skin model of IPC may provide new avenues for in vivo physiological, clinical and pharmacological research on mitochondrial metabolism.
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http://dx.doi.org/10.1111/bcp.14845DOI Listing
March 2021

Assessment of Microcirculatory Status Based on Stimulation of Myogenic Oscillations by Transient Ischemia: From Health to Disease.

Vasc Health Risk Manag 2021 10;17:33-36. Epub 2021 Feb 10.

Department of Athletics, Strength and Conditioning, Poznan University of Physical Education, Poznan, Poland.

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http://dx.doi.org/10.2147/VHRM.S292087DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7882797PMC
February 2021

Impact of chromosome 17 centromere copy number increase on patient survival and human epidermal growth factor receptor 2 expression in gastric adenocarcinoma.

Oncol Lett 2021 Feb 21;21(2):142. Epub 2020 Dec 21.

Department of Oncological Surgery, Gdynia Centre of Oncology, Pomeranian Hospitals, Gdynia, Pomeranian Voivodship 81-519, Poland.

The accurate evaluation of human epidermal growth factor receptor 2 (HER2) status is essential for the appropriate use of targeted therapies. An increased number of chromosome 17 centromere enumeration probe (CEP17) signals may underrate fluorescence hybridization (FISH) outcomes, resulting in false-negative or a false-equivocal HER2 status assessment. The aim of the present study was to assess the frequency of CEP17 copy number increase (CNI), its effects on HER2 protein expression (and the subsequent effects on tumor cells), and the survival outcomes of patients with gastric cancer. Archival primary tumor samples from 244 patients that underwent gastric resection for adenocarcinoma were retrieved for both HER2 protein expression analysis (using immunochemistry) and gene amplification (using FISH). The associations between HER2 status, CEP17 CNI and multiple clinicopathological parameters (including survival outcome), were assessed. The relationship between CEP17 CNI and HER2 protein upregulation was also investigated. CEP17 CNI was detected in 17.2% of cases, and a strong association between CEP17 CNI and HER2 upregulation was revealed. The impact of CEP17 CNI on survival did not reach statistical significance. Consequently, CEP17 CNI was discovered to be strongly associated with HER2 upregulation in tumor cells, which may characterize a critical issue in HER2 testing. Therefore, the eligibility for HER2-targeted agents in CEP17 CNI-positive patients warrants further recognition.
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http://dx.doi.org/10.3892/ol.2020.12403DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7798021PMC
February 2021

Adipose-derived stromal cells for nonhealing wounds: Emerging opportunities and challenges.

Med Res Rev 2021 Feb 1. Epub 2021 Feb 1.

Laboratory of Tissue Engineering and Regenerative Medicine, Department of Embryology, Medical University of Gdansk, Gdańsk, Poland.

Wound healing complications affect thousands of people each year, thus constituting a profound economic and medical burden. Chronic wounds are a highly complex problem that usually affects elderly patients as well as patients with comorbidities such as diabetes, cancer (surgery, radiotherapy/chemotherapy) or autoimmune diseases. Currently available methods of their treatment are not fully effective, so new solutions are constantly being sought. Cell-based therapies seem to have great potential for use in stimulating wound healing. In recent years, much effort has been focused on characterizing of adipose-derived mesenchymal stromal cells (AD-MSCs) and evaluating their clinical use in regenerative medicine and other medical fields. These cells are easily obtained in large amounts from adipose tissue and show a high proregenerative potential, mainly through paracrine activities. In this review, the process of healing acute and nonhealing (chronic) wounds is detailed, with a special attention paid to the wounds of patients with diabetes and cancer. In addition, the methods and technical aspects of AD-MSCs isolation, culture and transplantation in chronic wounds are described, and the characteristics, genetic stability and role of AD-MSCs in wound healing are also summarized. The biological properties of AD-MSCs isolated from subcutaneous and visceral adipose tissue are compared. Additionally, methods to increase their therapeutic potential as well as factors that may affect their biological functions are summarized. Finally, their therapeutic potential in the treatment of diabetic and oncological wounds is also discussed.
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http://dx.doi.org/10.1002/med.21789DOI Listing
February 2021

Development of a Peptide Derived from Platelet-Derived Growth Factor (PDGF-BB) into a Potential Drug Candidate for the Treatment of Wounds.

Adv Wound Care (New Rochelle) 2020 12 29;9(12):657-675. Epub 2019 Oct 29.

Laboratory of Tissue Engineering and Regenerative Medicine, Department of Embryology, Medical University of Gdansk, Gdansk, Poland.

This study evaluated the use of novel peptides derived from platelet-derived growth factor (PDGF-BB) as potential wound healing stimulants. One of the compounds (named PDGF2) was subjected for further research after cytotoxicity and proliferation assays on human skin cells. Further investigation included evaluation of: migration and chemotaxis of skin cells, immunological and allergic safety, the transcriptional analyses of adipose-derived stem cells (ASCs) and dermal fibroblasts stimulated with PDGF2, and the use of dorsal skin wound injury model to evaluate the effect of wound healing in mice. Colorimetric lactate dehydrogenase and tetrazolium assays were used to evaluate the cytotoxicity and the effect on proliferation. PDGF2 effect on migration and chemotaxis was also checked. Immunological safety and allergic potential were evaluated with a lymphocyte activation and basophil activation test. Transcriptional profiles of ASCs and primary fibroblasts were assessed after stimulation with PDGF2. Eight-week-old BALB/c female mice were used for dorsal skin wound injury model. PDGF2 showed low cytotoxicity, pro-proliferative effects on human skin cells, high immunological safety, and accelerated wound healing in mouse model. Furthermore, transcriptomic analysis of ASCs and fibroblasts revealed the activation of processes involved in wound healing and indicated its safety. A novel peptide derived from PDGF-BB was proved to be safe drug candidate in wound healing. We also present a multifaceted model for the initial screening of new compounds that may be potentially useful in wound healing stimulation. The results show that peptide derived from PDGF-BB is a promising drug candidate for wound treatment.
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http://dx.doi.org/10.1089/wound.2019.1051DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7698658PMC
December 2020

The Effect of Sports Rules Amendments on Exercise Intensity during Taekwondo-Specific Workouts.

Int J Environ Res Public Health 2020 09 17;17(18). Epub 2020 Sep 17.

Department of Athletics, Strength and Conditioning, Poznan University of Physical Education, ul. Królowej Jadwigi 27/39, 61-871 Poznań, Poland.

We aimed to compare the change in exercise response to taekwondo-specific circuit workouts before and after competition rule amendments. A total of 240 workouts in 15 elite athletes were analyzed over two years. Physiological and kinematic data were gathered with the wireless Bioharness system along with capillary blood samples for lactate concentration. Progressive exercise tests until exhaustion were periodically performed to obtain reference data. The rule changes resulted in significant increases (mainly medium or large effects) in the physiological (2.9-14.4%) and kinematic (4.8-10.1%) response to taekwondo-specific workouts. The largest increases were for peak breathing rate (12.0%), energy expenditure (6.6%), blood lactate immediately after exercise (10.2%) and at the 30th min of recovery (14.4%), and peak kinematic activity (10.1%). Significant differences between taekwondo-specific workouts and tournament combats persisted after the shift from old to new rules, ranging from 2.4 to 38.5% for physiological and from 2.9 to 15.5% for kinematic variables. The largest workout-combat differences were revealed for post-exercise (15.9%) and recovery (38.5%) blood lactate, peak (-15.8%) and relative (-15.0%) breathing rate, and mechanical (13.5%) and physiological (14.2%) intensity. Our study suggests that the rule amendments significantly modify the exercise response to discipline-specific workouts and that taekwondo-specific training sessions do not fully recreate the tournament demands in terms of physiological and kinematic load.
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http://dx.doi.org/10.3390/ijerph17186779DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7559273PMC
September 2020

Maximal Oxygen Uptake Adjusted for Skeletal Muscle Mass in Competitive Speed-Power and Endurance Male Athletes: Changes in a One-Year Training Cycle.

Int J Environ Res Public Health 2020 08 27;17(17). Epub 2020 Aug 27.

Department of Athletics, Strength and Conditioning, Faculty of Sport Sciences, Poznan University of Physical Education, ul. Królowej Jadwigi 27/39, 61-871 Poznań, Poland.

We compared the changes in maximum oxygen uptake (V·O2max) calculated per skeletal muscle mass (SMM) with conventional V·O2max measures in a 1-year training cycle. We hypothesized that the pattern of changes would differ between SMM-adjusted and absolute or weight-adjusted values, and the differences between groups of distinct training specialization and status will depend on the measure used. Twelve sprinters (24.7 ± 3.3 years), 10 endurance runners (25.3 ± 5.3 years), and 10 recreationally trained controls (29 ± 4.5 years) performed a treadmill test until exhaustion to determine V·O2max. Their SMM was estimated based on the dual X-ray absorptiometry method and a regression equation. The significance of differences was assessed using analysis of variance ( ≤ 0.05). The pattern of the longitudinal change was not different between V·O2max/SMM and standard measures. Also, the significance of differences between sprinters and endurance athletes remained similar regardless of the V·O2max measure. Sprinters and controls had similar absolute (~4.3 L·min) and total weight-adjusted (~52 vs. ~56 mL·min·kg) V·O2max, but they significantly differed in SMM-adjusted V·O2max (~110 vs. ~130 mL·min·kg SMM). In summary, SMM-adjusted V·O2max is not more useful than standard measures to track longitudinal changes in competitive athletes. However, it allows to better distinguish between groups or individuals differing in training status. The results of our study are limited to male athletes.
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http://dx.doi.org/10.3390/ijerph17176226DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7504314PMC
August 2020

Plasma Concentration of Irisin and Brain-Derived-Neurotrophic Factor and Their Association With the Level of Erythrocyte Adenine Nucleotides in Response to Long-Term Endurance Training at Rest and After a Single Bout of Exercise.

Front Physiol 2020 31;11:923. Epub 2020 Jul 31.

Department of Athletics, Strength and Conditioning, Poznań University of Physical Education, Poznań, Poland.

The study aimed to assess the effect of a single bout of incremental exercise on irisin and BDNF plasma concentrations as related to erythrocyte purine nucleotides concentration at rest and after exercise. Master endurance master athletes (training experience 38 ± 6 years) and a group of untrained participants completed a single bout of progressive incremental exercise test until exhaustion. The dual-energy x-ray absorptiometry and blood collection were performed. Blood was taken twice at rest and 10 min after exercise. Concentrations of ATP, ADP, and AMP were assessed in the erythrocytes. Hypoxanthine and uric acid were determined in plasma using the high-performance liquid chromatography. Plasma concentrations of irisin and BDNF were assessed through the immunoenzymatic method. The ATP level, ATP/ADP ratio and AEC value were significantly higher in the athletic group. A significantly higher concentration of BDNF was it also noted in the trained group that correlated with the erythrocyte energy status at rest. The single session of exercise induced a significant increase in ATP erythrocyte levels in both groups. Both exerkines significantly correlated at rest with red blood cell adenine nucleotides and degradation products (BDNF positively and irisin negatively). The blood concentration of BDNF and irisin, in response to exercise, was not significantly different between groups. Obtained data revealed a higher erythrocyte energy status and lower purine degradation products concentration in master athletes. Also resting plasma exerkines differed substantially between groups. In conclusion, long-term training resulted in exercise adaptation reflected by a higher erythrocyte energy status, lower purine degradation products concentration and modified concentration of exerkines (higher BDNF and lower irisin blood concentrations). Therefore, we consider the training-induced adaptations in master athletes to be beneficial and significant. The moderate level of physical activity in the untrained group, even if sufficient in terms of general health, did not cause any discernible changes.
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http://dx.doi.org/10.3389/fphys.2020.00923DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7411220PMC
July 2020

Imunofan-RDKVYR Peptide-Stimulates Skin Cell Proliferation and Promotes Tissue Repair.

Molecules 2020 Jun 23;25(12). Epub 2020 Jun 23.

Department of Biomedical Chemistry, Faculty of Chemistry, University of Gdańsk, 80-308 Gdańsk, Poland.

Regeneration and wound healing are vital to tissue homeostasis and organism survival. One of the biggest challenges of today's science and medicine is finding methods and factors to stimulate these processes in the human body. Effective solutions to promote regenerative responses will accelerate advances in tissue engineering, regenerative medicine, transplantology, and a number of other clinical specialties. In this study, we assessed the potential efficacy of a synthetic hexapeptide, RDKVYR, for the stimulation of tissue repair and wound healing. The hexapeptide is marketed under the name "Imunofan" (IM) as an immunostimulant. IM displayed stability in aqueous solutions, while in plasma it was rapidly bound by albumins. Structural analyses demonstrated the conformational flexibility of the peptide. Tests in human fibroblast and keratinocyte cell lines showed that IM exerted a statistically significant ( < 0.05) pro-proliferative activity (30-40% and 20-50% increase in proliferation of fibroblast and keratinocytes, respectively), revealed no cytotoxicity over a vast range of concentrations ( < 0.05), and had no allergic properties. IM was found to induce significant transcriptional responses, such as enhanced activity of genes involved in active DNA demethylation ( < 0.05) in fibroblasts and activation of genes involved in immune responses, migration, and chemotaxis in adipose-derived stem cells derived from surgery donors. Experiments in a model of ear pinna injury in mice indicated that IM moderately promoted tissue repair (8% in BALB/c and 36% in C57BL/6 in comparison to control).
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http://dx.doi.org/10.3390/molecules25122884DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7355430PMC
June 2020

The Effect of Training on Erythrocyte Energy Status and Plasma Purine Metabolites in Athletes.

Metabolites 2019 Dec 19;10(1). Epub 2019 Dec 19.

Department of Athletics, Strength and Conditioning, Poznan University of Physical Education, Królowej Jadwigi 27/39, 61-871 Poznan, Poland.

This study aimed to assess the changes in red blood cell (RBC) energy status and plasma purine metabolites concentration over a one-year training cycle in endurance-trained (EN; = 11, 20‒26 years), and sprint-trained (SP; = 11, 20-30 years) competitive athletes in comparison to recreationally-trained individuals (RE; = 11, 20‒26 years). Somatic, physiological, and biochemical variables were measured in four training phases differing in exercise load profile: transition, general, specific, and competition. Significantly highest values of RBC adenylate energy charge (AEC; ≤ 0.001), ATP-to-ADP and ADP-to-AMP ratios ( ≤ 0.05), and plasma levels of adenosine (Ado; ≤ 0.05) were noted in the competition phase in the EN and SP, but not in the RE group. Significantly lowest plasma levels of adenosine diphosphate (ADP; ≤ 0.05), adenosine monophosphate (AMP; ≤ 0.001), inosine (Ino; ≤ 0.001), and hypoxanthine (Hx; ≤ 0.001) accompanied by higher erythrocyte hypoxanthine-guanine phosphoribosyltransferase (HGPRT) activity ( ≤ 0.001), were observed in the competition phase in both athletic groups. No significant alterations were found in the erythrocyte concentration of guanine nucleotides in any group. In conclusion, periodized training of competitive athletes' results in a favorable adaptation of RBC metabolism. The observed changes cover improved RBC energy status (increased AEC and ATP/ADP ratio) and reduced purine loss with more efficient erythrocyte purine pool recovery (increased HGPRT activity and plasma levels of Ado; decreased Hx and Ino concentration).
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http://dx.doi.org/10.3390/metabo10010005DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7022963PMC
December 2019

The Physical Activity Questionnaire for the Elderly (PAQE): A Polish Adaptation.

Int J Environ Res Public Health 2019 12 6;16(24). Epub 2019 Dec 6.

Department of Physical Activity Sciences and Health Promotion, Poznan University of Physical Education, Królowej Jadwigi 27/39, 61-871 Poznań, Poland.

The aim of the study is to assess the reliability and validity of the Polish adaptation of the Physical Activity Questionnaire for the Elderly (PAQE-PL). One hundred and four older adults (75 women and 29 men) aged 65 to 89 (mean 72.2 ± 5.7 years) participated in the study. The test-retest procedure was used to evaluate the reliability of the PAQE-PL. Validity was assessed by comparing the results of the PAQE-PL with the measurements from an accelerometer (ActiGraph wGT3X+) and two questionnaires: the Polish version of the Community Healthy Activities Model Program for Seniors (CHAMPS-PL) and the Polish version of the Yale Physical Activity Survey (YPAS-PL). All test-retest interclass correlation coefficients (ICCs) were significant (ranged from 0.64 to 0.92). The long-term stability showed significant ICCs (ranged from 0.38 to 0.87) for all participants. In regard to validity, the obtained correlation coefficients were relatively low but statistically significant for all participants between the PAQE-PL scores and energy expenditure (r ranging from 0.25 to 0.26) measured by the accelerometer. The PAQE-PL correlated with almost all CHAMPS-PL indices, YPAS-PL energy expenditure, and total physical activity time. The results suggest that the adaptation of the PAQE-PL is an acceptable tool to estimate the physical activity level among older adults in the Polish population. We recommend the cautious and well-thought-out use of the PAQE-PL with a population of older women.
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http://dx.doi.org/10.3390/ijerph16244947DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6950819PMC
December 2019

Alterations in Exercise-Induced Plasma Adenosine Triphosphate Concentration in Highly Trained Athletes in a One-Year Training Cycle.

Metabolites 2019 Oct 16;9(10). Epub 2019 Oct 16.

Department of Athletics, Strength and Conditioning, Poznan University of Physical Education, Królowej Jadwigi 27/39, 61-871 Poznan, Poland.

This study aimed to assess the effect of training loads on plasma adenosine triphosphate responsiveness in highly trained athletes in a 1 y cycle. Highly trained futsal players (11 men, age range 20-31 y), endurance athletes (11 men, age range 18-31 y), sprinters (11 men, age range 21-30 y), and control group (11 men, age range 22-34 y) were examined across four characteristic training phases in response to an incremental treadmill test until exhaustion. A considerably higher exercise and post-exercise plasma adenosine triphosphate concentrations were observed in consecutive training phases in highly trained athletes, with the highest values reached after the competitive period. No differences in plasma adenosine triphosphate concentrations were found in the control group during the 1 y cycle. Sprinters showed a higher absolute and net increase in plasma adenosine triphosphate concentration by 60-114% during exercise in consecutive training phases than futsal players (63-101%) and endurance athletes (64-95%). In this study, we demonstrated that exercise-induced adenosine triphosphate concentration significantly changes in highly trained athletes over an annual training cycle. The obtained results showed that high-intensity but not low- to moderate-intensity training leads to an increased adenosine triphosphate response to exercise, suggesting an important role of ATP for vascular plasticity.
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http://dx.doi.org/10.3390/metabo9100230DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6836270PMC
October 2019

Change in Lactate, Ammonia, and Hypoxanthine Concentrations in a 1-Year Training Cycle in Highly Trained Athletes: Applying Biomarkers as Tools to Assess Training Status.

J Strength Cond Res 2020 Feb;34(2):355-364

Human Movement Laboratory "LABTHLETICS", Department of Athletics, Strength and Conditioning, Poznan University of Physical Education, Poznań, Poland.

Włodarczyk, M, Kusy, K, Słomińska, E, Krasiński, Z, and Zieliński, J. Change in lactate, ammonia, and hypoxanthine concentrations in a 1-year training cycle in highly trained athletes: applying biomarkers as tools to assess training status. J Strength Cond Res 34(2): 355-364, 2020-The aim was to determine changes in biomarker (LA, NH3, purine metabolites) blood concentration during graded exercise and recovery throughout an annual training cycle in highly trained athletes of different training profiles. The study included 12 sprinters (SP, 21-30 years), 11 triathletes (TR, 20-31 years), 12 futsal players (FU, 19-31 years), and 13 amateur runners (AM, 20-33 years). Purine metabolite (hypoxanthine, xanthine, uric acid), ammonia (NH3), and lactate (LA) concentrations were determined at rest, during an incremental treadmill exercise test (every 3 minutes), and during recovery (5, 10, 15, 20, and 30 minutes postexercise) in 4 phases of an annual training cycle. Purine metabolite concentration was determined from plasma, whereas LA and NH3 from whole blood. For LA during exercise and recovery, certain significant differences between training phases within groups were observed for FU, TR, and SP but not for AM. For NH3, the greatest differences between examination points were observed for SP and TR near maximal exercise and in the first few stages of recovery. For hypoxanthine (Hx), the largest amount of differences between examination points was observed for FU, TR, and FU throughout the entire exercise spectrum. Biomarker concentration dynamics change during an incremental exercise test and postexercise in an annual training cycle. Biomarker responses differ depending on training type and magnitude of training loads used in various phases of an annual training cycle. When assessing training status using an incremental exercise test throughout an annual training cycle, NH3 and Hx concentration changes are more sensitive compared with LA.
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http://dx.doi.org/10.1519/JSC.0000000000003375DOI Listing
February 2020

Surgical Site Infection after Breast Surgery: A Retrospective Analysis of 5-Year Postoperative Data from a Single Center in Poland.

Medicina (Kaunas) 2019 Aug 21;55(9). Epub 2019 Aug 21.

Department of Surgical Oncology, Medical University of Gdansk, 80-210 Gdansk, Poland.

Surgical site infection (SSI) is a significant complication of non-reconstructive and reconstructive breast surgery. This study aimed to assess SSI after breast surgery over five years in a single center in Poland. The microorganisms responsible for SSI and their antibiotic susceptibilities were determined. Data from 2129 patients acquired over five years postoperatively by the Department of Surgical Oncology, Medical University of Gdansk in Poland were analyzed. SSI was diagnosed in 132 patients (6.2%) and was an early infection in most cases (65.2%). The incidence of SSI was highest in patients who underwent subcutaneous amputation with simultaneous reconstruction using an artificial prosthesis (14.6%), and breast reconstruction via the transverse rectus abdominis muscle (TRAM) flap method (14.3%). Gram-positive bacteria were responsible for SSI in most cases (72.1%), and these were mainly strains (53.6%). These strains were 100% susceptible to all beta-lactam antibiotics (except penicillin) but were less susceptible to macrolides and lincosamides. SSI is a serious problem, and attention should be focused on its prevention. Reconstruction using an artificial prosthesis or via the TRAM flap method is connected to increased SSI incidence. Further studies are required to prevent SSI following breast surgery.
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http://dx.doi.org/10.3390/medicina55090512DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6780406PMC
August 2019

The Effect of Beta-Alanine versus Alkaline Agent Supplementation Combined with Branched-Chain Amino Acids and Creatine Malate in Highly-Trained Sprinters and Endurance Athletes: A Randomized Double-Blind Crossover Study.

Nutrients 2019 Aug 21;11(9). Epub 2019 Aug 21.

Department of Athletics, Strength and Conditioning, Poznan University of Physical Education, 61-871 Poznań, Poland.

The study aimed to verify the effect of intra- (beta-alanine-BA) versus extra- (alkaline agents-ALK) cellular buffering agent supplementation, combined with customarily used branched-chain amino acids (BCAAs) and creatine malate (TCM) treatment in natural training conditions. Thirty-one elite athletes (11 sprinters and 20 endurance athletes) participated in the study. Eight-week randomized double-blind, crossover, combined supplementation with BA-ALKpla and ALK-BApla was implemented. In the course of the experiment, body composition, aerobic capacity, and selected blood markers were assayed. After BA-ALKpla supplementation, total fat-free mass increased in sprinters ( = 0.009). No other differences were found in body composition, respiratory parameters, aerobic capacity, blood lactate concentration, and hematological indices after BA-ALKpla/ALK-BApla supplementation. The maximum post-exercise blood ammonia (NH) concentration decreased in both groups after BA-ALKpla supplementation (endurance, = 0.002; sprint, < 0.0001). Also, lower NH concentrations were observed in endurance athletes in the post-exercise recovery period. The results of our study indicate that combined BCAA, TCM, and BA supplementation is more effective than combined BCAA, TCM and ALK supplementation for an increase in fat-free mass and exercise adaptation, but not for aerobic capacity improvement. Besides, it seems that specific exercise stimuli and the training status are key factors affecting exercise performance, even in athletes using efficient supplementation.
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http://dx.doi.org/10.3390/nu11091961DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6769605PMC
August 2019

Purine metabolism in sprint- vs endurance-trained athletes aged 20‒90 years.

Sci Rep 2019 08 19;9(1):12075. Epub 2019 Aug 19.

Department of Athletics Strength and Conditioning, Poznan University of Physical Education, ul. Królowej Jadwigi 27/39, 61-871, Poznań, Poland.

Purine metabolism is crucial for efficient ATP resynthesis during exercise. The aim of this study was to assess the effect of lifelong exercise training on blood purine metabolites in ageing humans at rest and after exhausting exercise. Plasma concentrations of hypoxanthine (Hx), xanthine (X), uric acid (UA) and the activity of erythrocyte hypoxanthine-guanine phosphoribosyl transferase (HGPRT) were measured in 55 sprinters (SP, 20‒90 years), 91 endurance runners (ER, 20‒81 years) and 61 untrained participants (UT, 21‒69 years). SP had significantly lower levels of plasma purine metabolites and higher erythrocyte HGPRT activity than ER and UT. In all three groups, plasma purine levels (except UA in UT) significantly increased with age (1.8‒44.0% per decade). HGPRT activity increased in SP and ER (0.5‒1.0%), while it remained unchanged in UT. Hx and X concentrations increased faster with age than UA and HGPRT levels. In summary, plasma purine concentration increases with age, representing the depletion of skeletal muscle adenine nucleotide (AdN) pool. In highly-trained athletes, this disadvantageous effect is compensated by an increase in HGPRT activity, supporting the salvage pathway of the AdN pool restoration. Such a mechanism is absent in untrained individuals. Lifelong exercise, especially speed-power training, limits the age-related purine metabolism deterioration.
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http://dx.doi.org/10.1038/s41598-019-48633-zDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6700101PMC
August 2019

Wound healing complications in oncological patients: perspectives for cellular therapy.

Postepy Dermatol Alergol 2019 Apr 4;36(2):139-146. Epub 2018 Jan 4.

Department of Clinical Immunology and Transplantology, Medical University of Gdansk, Gdansk, Poland.

Various types of cancer are nowadays a serious medical and social problem and a great challenge for modern medicine. The majority of anticancer therapy is based on traditional chemotherapy and radiotherapy. Both of these highly non-specific types of treatment have a number of serious side effects including wound healing complications. Radiotherapy and chemotherapy mostly affect rapidly dividing skin cells (e.g. keratinocytes), as well as fibroblasts, melanocytes, endothelial and immune cells. Currently, there are many strategies to improve wound healing in oncological patients, including various types of dressings, biomaterials, growth factors, and cell therapies.
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http://dx.doi.org/10.5114/ada.2018.72585DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6627262PMC
April 2019

Polish Adaptation of the Yale Physical Activity Survey: Measurement Properties.

Int J Environ Res Public Health 2019 07 6;16(13). Epub 2019 Jul 6.

Department of Athletics, Strength and Conditioning, Faculty of Physical Education, Sport and Rehabilitation, Poznan University of Physical Education, 61-871 Poznan, Poland.

The aim of this study was to assess the measurement properties of a Polish adaptation of the Yale Physical Activity Survey (YPAS-PL). The Polish cultural adaptation of the YPAS was administered to a group of 104 people aged 65 to 89 years (mean age 72 ± 5). To assess the reliability of the YPAS-PL, a test-retest procedure was applied. Validity was assessed by comparing the results of the YPAS-PL with accelerometery (ActiGraph wGT3X+). The indicators based on the YPAS-PL activities checklist were characterized by high repeatability and had better reliability values than the YPAS-PL activity dimension indices (energy expenditure interclass correlation coefficient (ICC) = 0.81, total time physical activity ICC = 0.86). We noted a significant positive relationship between energy expenditure measured by an accelerometer and the YPAS-PL ( = 0.23). We can conclude that the YPAS-PL is an adequate tool for assessing energy expenditure related to physical activity in a Polish population of older adults. We also recommend the cautious and well thought-out use of the YPAS-PL activity dimension indices (summary, vigorous, leisurely walking, moving, standing, and sitting indexes).
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http://dx.doi.org/10.3390/ijerph16132401DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6651623PMC
July 2019

Combined Analysis of Blood Ammonia and Lactate Levels as a Practical Tool to Assess the Metabolic Response to Training Sessions in Male and Female Sprinters.

J Strength Cond Res 2019 Jul 1. Epub 2019 Jul 1.

Athletics, Strength and Conditioning, Poznan University of Physical Education, Poznan, Poland.

Kantanista, A, Kusy, K, Pospieszna, B, Korman, P, Wieliński, D, and Zieliński, J. Combined analysis of blood ammonia and lactate levels as a practical tool to assess the metabolic response to training sessions in male and female sprinters. J Strength Cond Res XX(X): 000-000, 2019-Previous research has mainly focused on blood ammonia and lactate concentration changes in response to exercise in laboratory settings. The aim of this study was to present a combined analysis of blood ammonia and lactate levels obtained during various training sessions performed under real training conditions. Differences between the sexes were also analyzed. The study subjects included 9 male and 8 female sprinters competing at the international level. The two-way analyses of variance, with repeated measures (time × sex), for lactate and blood ammonia concentrations during strength, speed (only lactate), speed with baton exchange, and speed endurance training sessions were significant. Blood ammonia and lactate levels obtained during repeated sprints were higher in male than female athletes. Peak lactate concentrations obtained from different training sessions were different in the female (F(3, 18) = 49.82, p ≤ 0.001, η = 0.893) and male (F(3, 21) = 312.26, p ≤ 0.001, η = 0.978) athletes; post hoc analyses of the men and women showed differences in maximum lactate concentration between training sessions, except in the speed and strength sessions. Peak ammonia concentrations obtained in the different training sessions were also different in the female (F(3, 18) = 121.06, p ≤ 0.001, η = 0.953) and male (F(3, 21) = 196.04, p ≤ 0.001, η = 0.965) athletes; in both the men and women, significant differences in maximum blood ammonia concentrations were found between the training sessions, except for the speed and speed with baton exchange training sessions. The results of this study indicate that the combined analysis of lactate and blood ammonia concentration provides the coach with valuable additional information about the level of adenosine triphosphate breakdown, the energy system contribution involved in muscle energy coverage during very short, repeated maximal sprints, and, most importantly, allows the coach to check whether preworkout goals were actually met.
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http://dx.doi.org/10.1519/JSC.0000000000003193DOI Listing
July 2019

Extremely High Mortality Rate after a Successful Gastrectomy for Cancer in Older Adults.

J Gastric Cancer 2019 Jun 9;19(2):202-211. Epub 2019 May 9.

Department of Oncological Surgery, Medical University of Gdańsk, Gdańsk, Poland.

Purpose: Poor physiological reserve for withstanding major cancer surgery in older adults is an important concern in the selection of patients for oncologic gastrectomy. The present study aimed to analyze mortality patterns among patients who underwent gastrectomy for cancer according to age groups. The primary outcomes of this study were early- and middle-term results: 30-day and 3-, 6-, 12-, and 36-month mortality rates.

Materials And Methods: A retrospective review of 288 patients who underwent surgical resection for gastric cancer in two centers was carried out. Patients were stratified into four groups according to age: 29-50 years (group I, n=27), 51-65 years (group II, n=117), 66-75 years (group III, n=81), and 76-92 years (group IV, n=58). Statistical calculations focused on the differences in the survival rates between groups I and II as well as between groups II and IV.

Results: The middle-aged patients (group II) had significantly better 3-year survival than either the youngest (group I) or the oldest patients (group IV). The 6-month mortality rates were 16.9% in group III and 29.3% in group IV. Two-thirds of the patients from groups III and IV who died between 2 and 6 months after surgery had an uneventful postoperative course.

Conclusions: Age is an important prognostic factor of middle-term survival after gastrectomy for cancer. Geriatric assessment and better patient selection for major surgery for cancer are required to improve the outcome of gastrectomy for cancer in patients aged over 75 years.
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http://dx.doi.org/10.5230/jgc.2019.19.e16DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6589425PMC
June 2019

The Effect of Exercise on the Skin Content of the Reduced Form of NAD and Its Response to Transient Ischemia and Reperfusion in Highly Trained Athletes.

Front Physiol 2019 15;10:600. Epub 2019 May 15.

Department of Cardiology-Intensive Therapy, Poznań University of Medical Sciences, Poznań, Poland.

Reduced nicotinamide adenine dinucleotide (NADH) is synthesized in the cellular nucleus, cytoplasm and mitochondria but oxidized into NAD almost exclusively in mitochondria. Activation of human skin by the 340 nm ultraviolet light triggers natural fluorescence at the light length of 460 nm, which intensity is proportional to the skin NADH content. This phenomenon is used by the Flow Mediated Skin Fluorescence (FMSF) which measures changes in the skin NADH content during transient ischemia and reperfusion. We examined the effects of exercise to exhaustion on the skin changes of NADH in response to 200 s forearm ischemia and reperfusion in 121 highly trained athletes (94 men and 27 women, long-distance running, triathlon, taekwondo, rowing, futsal, sprint running, fencing, and tennis). We found that exercise until exhaustion changes the skin content of NADH, modifies NADH turnover at rest, during ischemia and reperfusion in the most superficial living skin cells. Compared to the pre-exercise, there were significant increases in: mean fluorescence recorded during rest as the baseline value () ( < 0.001), the maximal fluorescence that increased above the baseline during controlled forearm ischemia (FI) ( < 0.001, only in men), the minimal fluorescence after decreasing below the baseline during reperfusion (FR) ( < 0.001 men; < 0.01 women) and the difference between and FR () ( < 0.01), and reductions in the difference between FI and () ( < 0.001) and /IR ratio (CI) ( < 0.001) after the incremental exercise test. There was no statistical difference between pre- and post-exercise the maximal range of the fluorescence change during ischemia and reperfusion (IR) In conclusion, exercise to exhaustion modifies the skin NADH content at rest, during ischemia and reperfusion as well as the magnitude of changes in the NADH caused by ischemia and reperfusion. Our findings suggest that metabolic changes in the skin NADH accompanying exercise extend beyond muscles and affect other cells and organs.
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http://dx.doi.org/10.3389/fphys.2019.00600DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6529559PMC
May 2019

Validity and Reliability of the Polish Adaptation of the CHAMPS Physical Activity Questionnaire.

Biomed Res Int 2019 28;2019:6187616. Epub 2019 Mar 28.

Department of Physical Education and Lifelong Sports, Faculty of Physical Education, Sport and Rehabilitation, Poznan University of Physical Education, Królowej Jadwigi 27/39, 61-871 Poznań, Poland.

The aim of the study was to investigate the reliability and construct validity of the Polish adaptation of the Community Health Activities Model Program for Seniors (CHAMPS) physical activity questionnaire among the elderly. The sample included 104 volunteers, 75 women (age = 71.0 ± 5.0 years) and 29 men (age = 75.1 ± 6.6 years). To assess the reliability of the Polish version of the CHAMPS physical activity questionnaire, measurements were conducted by one-week test-retest. The construct validity of the CHAMPS physical activity questionnaire was evaluated using accelerometers. Criterion validation was verified by self-reported measurements (health self-assessment, life satisfaction, and wellbeing) and body composition analysis. Intraclass correlation coefficients of the one-week test-retest ranged from 0.79 to 0.85. Significant Pearson's correlations were found between caloric expenditure measured by accelerometer and CHAMPS caloric expenditure in all listed physical activities ( = 0.33) and caloric expenditure in at least moderate intensity physical activities ( = 0.37) of the CHAMPS physical activity questionnaire. Moderate and greater intensity physical activities of CHAMPS measure were significantly related to total bone mass, health self-assessment, life satisfaction as a whole, and personal wellbeing ( ranged from 0.26 to 0.34). The findings of the study allow us to conclude that the Polish version of the CHAMPS physical activity questionnaire has acceptable reliability and validity to assess physical activity of older adults.
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http://dx.doi.org/10.1155/2019/6187616DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6458940PMC
August 2019

Changes in Blood Concentration of Adenosine Triphosphate Metabolism Biomarkers During Incremental Exercise in Highly Trained Athletes of Different Sport Specializations.

J Strength Cond Res 2019 May;33(5):1192-1200

Human Movement Laboratory "LABTHLETICS", Department of Athletics, Strength and Conditioning, Poznan University of Physical Education, Poznań, Poland.

Włodarczyk, M, Kusy, K, Słomińska, E, Krasiński, Z, and Zieliński, J. Changes in blood concentration of adenosine triphosphate metabolism biomarkers during incremental exercise in highly trained athletes of different sport specializations. J Strength Cond Res 33(5): 1192-1200, 2019-We hypothesized that (a) high-level specialized sport training causes different adaptations that induce specific biomarker release dynamics during exercise and recovery and (b) skeletal muscle mass affects biomarker release. Eleven sprinters (21-30 years), 16 endurance runners (18-31 years), 12 futsal players (18-29 years), and 12 amateur runners as controls (22-33 years) were examined. Hypoxanthine (Hx), xanthine (X), uric acid (UA), ammonia (NH3), and lactate (LA) concentrations were determined at rest, during an incremental treadmill exercise test (every 3 minutes), and during recovery (5, 10, 15, 20, and 30 minutes after exercise). Hx, X, and UA concentration was determined from plasma, while LA and NH3 from whole blood, and muscle mass was assessed using dual X-ray absorptiometry method. At rest, during incremental exercise, and up to 30 minutes into the postexercise recovery period, sprinters had lowest Hx, X, and UA concentrations, and endurance athletes had lowest NH3 concentrations. For LA during exercise, the lowest concentrations were noted in endurance athletes, except when reaching maximum intensity, where the differences between groups were not significant. There were no significant correlations observed between skeletal muscle mass and biomarker concentration at maximal intensity and recovery in any group. In conclusion, the magnitude of exercise-induced biomarker concentration is only related to training adaptations through specific training profile but not to muscle mass. In addition, the results suggest that combined measuring of LA, NH3, and Hx concentration in blood is useful in indirectly reflecting key changes in exercise- and training-induced energy status. Further research should focus on studying how specific training sessions affect individual biomarker response in highly trained athletes.
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http://dx.doi.org/10.1519/JSC.0000000000003133DOI Listing
May 2019

Transcriptional activity of epigenetic remodeling genes declines in keratinocytes after in vitro expansion.

Adv Med Sci 2019 Sep 19;64(2):274-279. Epub 2019 Mar 19.

Laboratory of Tissue Engineering and Regenerative Medicine, Department of Embryology, Faculty of Medicine, Medical University of Gdańsk, Gdańsk, Poland. Electronic address:

Purpose: In vitro expansion is an invaluable method to obtain keratinocytes in amounts necessary for effective transplantation therapies. In vitro cell culturing provokes questions concerning potential epigenetic alterations occurring in expanded cells in the context of usefulness for transplantation and safety. The purpose of this study was to investigate as to whether keratinocyte expansion is associated with changes in the activity of genes responsible for the maintenance of epigenetic stability.

Materials And Methods: We focussed on the transcriptional activity of genes involved in different epigenetic mechanisms including DNA methylation and histone modifications. We used quantitative real-time PCR to determine transcript levels of 16 epigenetic remodelling markers in 14 patients in the epidermal cells directly after collection and after in vitro expansion.

Results: We observed a remarkable decline in the transcriptional activity of the epigenetic remodelling genes following in vitro expansion, while no further fall of expression with passaging. In whole skin, we found even higher expression levels of the epigenetic markers.

Conclusions: Transmission to in vitro environment challenges cellular signalling and metabolism. The regulation of epigenetic remodelling maintains the balance between cellular plasticity and phenotype deviation. This preliminary research demonstrated reduced activity of genes responsible for epigenetic modifications of DNA and histones in in vitro expanded epidermal cells. This observation indicates that epigenome re-patterning in cultured epidermal cells is significantly less intensive than in the skin. Also, this observation may imply that after adaptation to in vitro conditions, the epigenome does not undergo extensive transformation during further cultivation.
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http://dx.doi.org/10.1016/j.advms.2019.03.001DOI Listing
September 2019

Exercise Response to Real Combat in Elite Taekwondo Athletes Before and After Competition Rule Changes.

J Strength Cond Res 2019 Mar 4. Epub 2019 Mar 4.

Department of Athletics, Strength and Conditioning, Poznan University of Physical Education, Poznań, Poland.

Janowski, M, Zieliński, J, and Kusy, K. Exercise response to real combat in elite taekwondo athletes before and after competition rule changes. J Strength Cond Res XX(X): 000-000, 2019-We hypothesize that recent (2017) changes in competition rules significantly affected kinematic (mechanical activity) and physiological (cardiopulmonary indices and lactate concentration) profile of the taekwondo combat, which is currently becoming more demanding in terms of exercise intensity and fatigue. Twenty two male and female elite taekwondo athletes were followed up for over 2 years. In total, 258 real tournament combats were included in the analysis (133 in old rules and 125 in new rules). Kinematic and physiological characteristics were recorded using a biomonitor (BioHarness 3; Zephyr Technologies) worn by athletes during tournaments. Blood samples were drawn after each combat for lactate concentration. Our research showed that recent amendments of competition rules were linked to a noticeable shift toward higher kinematic output and, consequently, increased physiological response. Significant increments in kinematic variables (3-8%), heart rate (HR) (1.5-1.8%), energy expenditure (EE) (3-5%), overall physiological load (2-4%), and lactate concentration (∼15% immediately after exercise and ∼25% in recovery) suggest that new rules are more demanding, although the statistical effect size is only small or moderate. In conclusion, after competition rule changes, there has been a shift in taekwondo combat profile toward greater body movement dynamics, higher intensity, and greater postexercise fatigue. The values of key indices of exercise response (mechanical activity, HR, EE, and lactate concentration) during tournaments are near or exceeding the maximum attained during progressive exercise until exhaustion. Therefore, more focus is needed on taekwondo-specific high-intensity training and postcombat recovery to adapt athletes to increased competition requirements.
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http://dx.doi.org/10.1519/JSC.0000000000003110DOI Listing
March 2019

Decreased Bone Mineral Density in Forearm vs Loaded Skeletal Sites in Professional Ballet Dancers.

Med Probl Perform Art 2019 Mar;34(1):25-32

Dep. of Biomechanics, Poznań University of Physical Education, ul. Królowej Jadwigi 27/39, 61-871 Poznań, Poland. Tel +48 61 835 53 91.

Aims: To compare the differences in bone mineral density (BMD) at loaded and non-loaded skeletal sites in professional ballet dancers. We hypothesized that in both male and female elite ballet dancers, a significant difference in BMD will be observed between impact and non-impact sites.

Methods: 44 elite ballet dancers, 22 men (age 26.4±5.9 yrs) and 22 women (age 24.9±5.3 yrs), were examined. BMD measurements were performed using dual-energy x-ray absorptiometry at three skeletal sites-forearm (FA), lumbar spine (LS), and femoral neck (FN)-and analyzed using t-tests, ANOVA, and linear regression models. Information about career duration, training volume, health habits, and menstrual disorders (women) was collected.

Results: Z-scores for LS and FN were significantly higher in men than in women. However, Z-scores for FA were similar in men and women and fell below the expected range for age (<-2.0), and they were significantly lower than those for LS and FN. With longer career duration, a trend was observed towards lower Z-scores for FN in men and towards higher Z-scores for FA in women.

Conclusion: In ballet dancers, FA mineralization is extremely low compared to loaded skeletal sites. Male dancers may have lowered forearm BMD despite the absence of risk factors present in female dancers (menstrual disorders). Professional ballet dancers may be at risk of local osteopenia due to the "local non-impact" characteristics of ballet dance, and use of the 33% distal radius region for the accurate assessment of bone mineral status should be investigated further in this population.
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http://dx.doi.org/10.21091/mppa.2019.1006DOI Listing
March 2019

Plasma Nucleotide Dynamics during Exercise and Recovery in Highly Trained Athletes and Recreationally Active Individuals.

Biomed Res Int 2018 9;2018:4081802. Epub 2018 Oct 9.

Department of Athletics, Strength and Conditioning, Poznan University of Physical Education, Królowej Jadwigi 27/39, 61-871 Poznań, Poland.

Circulating plasma ATP is able to regulate local skeletal muscle blood flow and 0 delivery causing considerable vasodilatation during exercise. We hypothesized that sport specialization and specific long-term training stimuli have an impact on venous plasma [ATP] and other nucleotides concentration. Four athletic groups consisting of sprinters (n=11; age range 21-30 yr), endurance-trained athletes (n=16; age range 18-31 yr), futsal players (n=14; age range 18-30 yr), and recreationally active individuals (n=12; age range 22-33 yr) were studied. Venous blood samples were collected at rest, during an incremental treadmill test, and during recovery. Baseline [ATP] was 759±80 nmol·l in competitive athletes and 680±73 nmol·l in controls and increased during exercise by ~61% in competitive athletes and by ~31% in recreationally active participants. We demonstrated a rapid increase in plasma [ATP] at exercise intensities of 83-87% of VOmax in competitive athletes and 94% in controls. Concentrations reported after 30 minutes of recovery were distinct from those obtained preexercise in competitive athletes ( < 0.001) but not in controls ( = 0.61). We found a correlation between total-body skeletal muscle mass and resting and maximal plasma [ATP] in competitive athletes (r=0.81 and r=0.75, respectively). In conclusion, sport specialization is significantly related to plasma [ATP] at rest, during exercise, and during maximal effort. Intensified exercise-induced plasma [ATP] increases may contribute to more effective vessel dilatation during exercise in highly trained athletes than in recreational runners. The most rapid increase in ATP concentration was associated with the respiratory compensation point. No differences between groups of competitive athletes were observed during the recovery period suggesting a similar pattern of response after exercise. Total-body skeletal muscle mass is indirectly related to plasma [ATP] in highly trained athletes.
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http://dx.doi.org/10.1155/2018/4081802DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6198572PMC
February 2019

The relationship between HER2 overexpression and angiogenesis in gastric cancer.

Medicine (Baltimore) 2018 Oct;97(42):e12854

Department of Oncological Surgery, Gdynia Oncology Centre, Gdynia.

In gastric cancer, HER2 protein overexpression is considered to be conducive to the higher proliferation activity of the tumor cells. Tumor formation is associated with angiogenesis in order to secure an abundant supply of oxygen and glucose to cancer cells. The aim of the study was to assess if HER2 overexpression is related to higher microvessel density (MVD) in the tumor stroma.The archival samples of primary tumor from 144 consecutive patients that underwent gastric resection for cancer between August 1, 2006 and December 31, 2013 in the Department of Oncological Surgery of Medical University of Gdańsk were analyzed. CD34 was used as a marker of MVD in the tumor stroma. Both CD34 and HER2 protein expressions were tested by immunohistochemistry.The assays were unsuccessful to estimate HER2 in 10 cases and CD34 in 14 cases due to technical reasons. The results were obtained for 128 patients. HER2 0 and HER2 1+ were considered negative, while HER2+ and HER2 3+ were recognized as positive. Mean MVD (mean number of vessels in the visual field) was 32.4 (median 29.5). Microvessel density was insignificantly higher in HER2 positive tumors. The slight difference was also seen between IHC 2+ and 3+ groups. The differences did not reach the level of statistical significance.Statistical analysis performed in our study did not reveal the significant relationship between HER2 overexpression on the tumor cells and MVD in the tumor stroma.
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http://dx.doi.org/10.1097/MD.0000000000012854DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6211927PMC
October 2018

Immunophenotyping and transcriptional profiling of in vitro cultured human adipose tissue derived stem cells.

Sci Rep 2018 07 27;8(1):11339. Epub 2018 Jul 27.

Faculty of Pharmacy, Medical University of Gdansk, Gdansk, Poland.

Adipose-derived stem cells (ASCs) have become an important research model in regenerative medicine. However, there are controversies regarding the impact of prolonged cell culture on the ASCs phenotype and their differentiation potential. Hence, we studied 10 clinical ASCs replicates from plastic and oncological surgery patients, in six-passage FBS supplemented cultures. We quantified basic mesenchymal cell surface marker transcripts and the encoded proteins after each passage. In parallel, we investigated the differentiation potential of ASCs into chondrocytes, osteocytes and adipocytes. We further determined the effects of FBS supplementation and subsequent deprivation on the whole transcriptome by comprehensive mRNA and miRNA sequencing. Our results show that ASCs maintain differentiation potential and consistent profile of key mesenchymal markers, with apparent expression of distinct isoforms, in long-term cultures. No significant differences were observed between plastic and oncological surgery cohorts. ASCs in FBS supplemented primary cultures are almost committed to mesenchymal lineages as they express key epithelial-mesenchymal transition genes including early mesenchymal markers. Furthermore, combined mRNA/miRNA expression profiling strongly supports a modulatory role for the miR-30 family in the commitment process to mesenchymal lineages. Finally, we propose improvements to existing qPCR based assays that address alternative isoform expression of mesenchymal markers.
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http://dx.doi.org/10.1038/s41598-018-29477-5DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6063933PMC
July 2018

Use of fiberoductoscopy for the management of patients with pathological nipple discharge: experience of a single center in Poland.

Breast Cancer 2018 Nov 25;25(6):753-758. Epub 2018 Jun 25.

Department of Surgical Oncology, Medical University of Gdansk, Smoluchowski Str 17, 80-214, Gdansk, Poland.

Background: Pathological nipple discharge (PND) is associated with serious clinical and diagnostic issues. Fiberoductoscopy (FDS) is a new diagnostic option in PND patients. This study summarizes our initial experience of FDS for the management of PND patients in a single center in Poland and assesses its safety.

Methods: A total of 256 women with PND were included in this prospective, case-controlled, single-center study between 2006 and 2014. Of the 250 patients who underwent FDS, 154 had mammary duct lesions and 96 had no visible lesions. Subsequently, 129 patients with lesions identified by FDS underwent microductectomy and the lesions were pathologically evaluated.

Results: The mean duration of FDS examination was 17 min. The most frequent intraductal lesion was amputation of a duct (35.1%), followed by circular narrowing or hyperplasia (22.7%). Final histological findings were unremarkable in 11.6% of cases, whereas mammary duct papilloma, duct ectasia, and ductal carcinoma in situ were detected in 71.3, 10.9, and 6.2% of cases, respectively.

Conclusions: FDS is an innovative method for visualizing intraductal mammary lesions and allows accurate selection of mammary ducts with suspicious lesions that require surgical removal in women with PND.
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http://dx.doi.org/10.1007/s12282-018-0883-3DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6208849PMC
November 2018