Publications by authors named "J Q Liu"

95,002 Publications

Self-Powered Biosensor for Specifically Detecting Creatinine in Real Time Based on the Piezo-Enzymatic-Reaction Effect of Enzyme-Modified ZnO Nanowires.

Biosensors (Basel) 2021 Sep 16;11(9). Epub 2021 Sep 16.

College of Sciences, Northeastern University, Shenyang 110819, China.

Creatinine has become an important indicator for the early detection of uremia. However, due to the disadvantages of external power supply and large volume, some commercial devices for detecting creatinine concentration have lost a lot of popularity in everyday life. This paper describes the development of a self-powered biosensor for detecting creatinine in sweat. The biosensor can detect human creatinine levels in real time without the need for an external power source, providing information about the body's overall health. The piezoelectric output voltage of creatininase/creatinase/sarcosine oxidase-modified ZnO nanowires (NWs) is significantly dependent on the creatinine concentration due to the coupling effect of the piezoelectric effect and enzymatic reaction (piezo-enzymatic-reaction effect), which can be regarded as both electrical energy and biosensing signal. Our results can be used for the detection of creatinine levels in the human body and have great potential in the prediction of related diseases.
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http://dx.doi.org/10.3390/bios11090342DOI Listing
September 2021

Baicalin inhibits biofilm formation by influencing primary adhesion and aggregation phases in Staphylococcus saprophyticus.

Vet Microbiol 2021 Sep 23;262:109242. Epub 2021 Sep 23.

MOE Joint International Research Laboratory of Animal Health and Food Safety and Traditional Chinese Veterinary Medicine Research Center, College of Veterinary Medicine, Nanjing Agricultural University, Nanjing 210095, PR China. Electronic address:

The ability to form biofilms on surfaces makes Staphylococcus saprophyticus (S. saprophyticus) becomes the main pathogenic factor in nosocomial infections. Previously, we demonstrated that baicalin (Bac) inhibited azithromycin-resistant S. saprophyticus (ARSS) biofilm formation. This investigation aims to explore the influence of baicalin on primary adhesion and aggregation phases of biofilm formation, and the treatment effect of baicalin and azithromycin on ARSS biofilm-associated infection. Crystal violet (CV) staining and scanning electron microscope (SEM) observations clearly showed that sub-inhibitory concentration baicalin inhibited ARSS biofilm formation when baicalin was added before the adhesion and aggregation phases. Baicalin significantly increased the relative adhesion inhibition rate and decreased the rate of bacteria aggregation in a dose-dependent manner. Moreover, CLSM and cell lysis assays revealed that baicalin inhibited the production of surface proteins and cell autolysis in bacteria adhesion and aggregation phases of biofilm formation. Meanwhile, the relative expressions of adhesion-related and autolysis-related genes were down-regulated by baicalin. In vivo, the combination of baicalin and azithromycin succeeded in eradicating ARSS from the mouse cutaneous infection model and decreasing the pathological injuries, the expressions of cytokines in infected tissue, and the number of inflammatory cells in the blood. Simultaneously, baicalin decreased the bacterial burdens in tubes, the level of TNF-α, and the number of monocytes and neutrophils compared with that of the SS and azithromycin groups. Based on these results, baicalin inhibited the adhesion and aggregation phases of biofilm formation by influenced the production of surface proteins and cell autolysis. Baicalin and azithromycin synergetically treated ARSS biofilm-associated infection.
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http://dx.doi.org/10.1016/j.vetmic.2021.109242DOI Listing
September 2021

Lanostane triterpenoids from cultivated fruiting bodies of Ganoderma sichuanense: Determination of the C-25 absolute configuration of ganoderic acid A and its derivatives using the phenylglycine methyl ester (PGME) method.

Phytochemistry 2021 Sep 21;192:112963. Epub 2021 Sep 21.

National Center for Genetic Engineering and Biotechnology (BIOTEC), 113 Thailand Science Park, Phaholyothin Road, Klong Luang, Pathumthani, 12120, Thailand. Electronic address:

Three undescribed lanostane triterpenoids, together with twenty-one known compounds, were isolated from artificially cultivated fruiting bodies of the basidiomycete Ganoderma sichuanense. The absolute configuration at C-25 of ganoderic acid A and its derivatives was determined to be 25R by application of the phenylglycine methyl ester (PGME) method. Among the isolated compounds, ganoderiol F exhibited the most potent activity against Mycobacterium tuberculosis H37Ra with an MIC value of 0.781 μg/ml.
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http://dx.doi.org/10.1016/j.phytochem.2021.112963DOI Listing
September 2021

The pharmacokinetic challenge of polymyxin B in critically ill patients with morbid obesity.

J Glob Antimicrob Resist 2021 Sep 22. Epub 2021 Sep 22.

Department of Pharmacy, The Third Xiangya Hospital of Central South University, Changsha, Hunan, 410013, China; Department of Pharmacy and Center of Clinical Pharmacology, The Third Xiangya Hospital of Central South University, Changsha, Hunan, 410013, China. Electronic address:

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http://dx.doi.org/10.1016/j.jgar.2021.09.004DOI Listing
September 2021

Fate of adipocyte progenitors during adipogenesis in mice fed a high-fat diet.

Mol Metab 2021 Sep 23:101328. Epub 2021 Sep 23.

First Department of Internal Medicine, Faculty of Medicine, University of Toyama, 2630 Sugitani, Toyama, 930-0194, Japan. Electronic address:

Objective: Expansion of adipose tissue during obesity through the recruitment of newly generated adipocytes (hyperplasia) is metabolically healthy, whereas that through the enlargement of pre-existing adipocytes (hypertrophy) leads to metabolic complications. Accumulating evidence from genetic fate mapping studies suggests that in animal models receiving a high-fat diet (HFD), only adipocyte progenitors (APs) in gonadal white adipose tissue (gWAT) have proliferative potential. However, the proliferative potential and differentiating capacity of APs in the inguinal WAT (iWAT) of male mice remains controversial. The objective of this study was to investigate the proliferative and adipogenic potential of APs in the iWAT of HFD-fed male mice.

Methods: We generated PDGFRα-GFP-CreER/tdTomato (KI/td) mice and traced PRGFRα-positive APs in male mice fed HFD for 8 weeks. We performed a comprehensive phenotypic analysis, including the histology, immunohistochemistry, flow cytometry, and gene expression analysis, of KI/td mice fed HFD.

Results: Contrary to the findings of others, we found an increased number of newly generated tdTomato adipocytes in the iWAT of male mice, which was smaller than that observed in the gWAT. We found that in male mice, the iWAT has more proliferating tdTomato APs than the gWAT. We also found that tdTomato APs showed a higher expression of Dpp4 and Pi16 than tdTomato APs, and the expression of these genes was significantly higher in the iWAT than in the gWAT of mice fed HFD for 8 weeks. Collectively, our results reveal that HFD feeding induces the proliferation of tdTomato APs in the iWAT of male mice.

Conclusion: In male mice, compared with gWAT, iWAT undergoes hyperplasia in response to 8 weeks of HFD feeding through the recruitment of newly generated adipocytes due to an abundance of APs with a high potential for proliferation and differentiation.
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http://dx.doi.org/10.1016/j.molmet.2021.101328DOI Listing
September 2021
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