Publications by authors named "J M Ruiz Sánchez"

5,312 Publications

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A multi-herd study shows that saliva is more than a reflection of serum biomarkers in pigs.

Animal 2021 Nov 26;15(12):100413. Epub 2021 Nov 26.

BioVetMed Research Group, Department of Animal Medicine and Surgery, Veterinary School, CEIR Campus Mare Nostrum (CMN), University of Murcia, 30100 Espinardo, Murcia, Spain. Electronic address:

This study evaluates if biomarkers of porcine health status in saliva samples is a mere reflection of serum to detect disease in pigs under field conditions. Four farms from the same commercial company were included to obtain samples from animals with different pathological conditions. A total of 10 healthy animals and 10-15 animals from each farm with clinical symptoms of the disease were sampled for paired saliva and blood during a veterinary clinical visit. The biomarker panel included acute-phase proteins (APPs), C-reactive protein (CRP), haptoglobin (Hp), an inflammatory marker, adenosine deaminase (ADA), the total antioxidant capacity (TAC), the levels of essential trace elements, copper (Cu) and zinc (Zn), and the measurement of the total protein content (TP). After detailed statistical analysis, the results showed that saliva could replace serum for APP measurements since a good agreement has been observed between the concentrations of APPs in both body fluids. For any other biomarker, no agreement between the concentrations quantified in serum and saliva samples was observed visually. However, salivary ADA and TP concentrations were statistically significantly higher in the diseased, whereas the statistical tests with serum concentrations were inconclusive. Furthermore, greater differentiation between healthy and diseased animals could be observed when the distribution of biomarkers was analysed in saliva than in other serum samples. The diagnostic power to discriminate between healthy and diseased pigs is similar in saliva and in serum samples. Preliminary regression models may offer an optimal combination of biomarkers for disease detection in saliva (Hp, CRP, and TAC) and serum (Hp, CRP, and Cu), which demands less labour, sample, and financial cost for saliva determinations. The contradictory results observed for TAC, Cu, and Zn levels between body fluids indicate a need for further studies. To sum up, saliva-based biomarkers instead of serum-based biomarkers could contribute to more efficient detection of diseased animals.
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http://dx.doi.org/10.1016/j.animal.2021.100413DOI Listing
November 2021

On the Role of the Conserved Histidine at the Chromophore Isomerization Site in Phytochromes.

J Phys Chem B 2021 Nov 29. Epub 2021 Nov 29.

Institut für Chemie, Technische Universität Berlin, Sekr. PC14, Straße des 17. Juni 135, D-10623 Berlin, Germany.

Phytochromes are sensory photoreceptors that use light to drive protein structural changes, which in turn trigger physiological reaction cascades. The process starts with a double-bond photoisomerization of the linear methine-bridged tetrapyrrole chromophore in the photosensory core module. The molecular mechanism of the photoconversion depends on the structural and electrostatic properties of the chromophore environment, which are highly conserved in related phytochromes. However, the specific role of individual amino acids is yet not clear. A histidine in the vicinity of the isomerization site is highly conserved and almost invariant among all phytochromes. The present study aimed at analyzing its role by taking advantage of a myxobacterial phytochrome SaBphP1 from , where this histidine is naturally substituted with a threonine (Thr289), and comparing it to its normal, His-containing counterpart from the same organism SaBphP2 (His275). We have carried out a detailed resonance Raman and IR spectroscopic investigation of the wild-type proteins and their respective His- or Thr-substituted variants (SaBphP1-T289H and SaBphP2-H275T) using the well-characterized prototypical phytochrome Agp1 from as a reference. The overall mechanism of the photoconversion is insensitive toward the His substitution. However, the chromophore geometry at the isomerization site appears to be affected, with a slightly stronger twist of ring D in the presence of Thr, which is sufficient to cause different light absorption properties in SaBphP1 and SaBphP2. Furthermore, the presence of His allows for multiple hydrogen-bonding interactions with the ring D carbonyl which may be the origin for the geometric differences of the C-D methine bridge compared to the Thr-containing variants. Other structural and mechanistic differences are independent of the presence of His. The most striking finding is the protonation of the ring C propionate in the Pfr states of SaBphP2, which is common among bathy phytochromes but so far has not been reported in prototypical phytochromes.
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http://dx.doi.org/10.1021/acs.jpcb.1c08245DOI Listing
November 2021

Exploration of Copper Oxide Nanoneedle Electrosynthesis Applied in the Degradation of Methylene Blue.

Nanomaterials (Basel) 2021 Nov 8;11(11). Epub 2021 Nov 8.

Instituto de Ciencias Naturales, Facultad de Medicina Veterinaria y Agronomía, Universidad de Las Americas, Manuel Montt 948, Santiago 7500975, Chile.

In this study, we report a low cost, fast and unexplored electrochemical synthesis strategy of copper oxide nanoneedles films as well as their morphological and chemical characterization. The nanostructured films were prepared using electrochemical anodization in alkaline electrolyte solutions of ethylene glycol, water and fluoride ions. The film morphology shows nanoneedle-shaped structures, with lengths up to 1-2 μm; meanwhile, high-resolution X-ray photoelectron spectroscopy (HRXPS) and spectroscopy Raman analyses indicate that a mixture of Cu(II) and Cu(I) oxides, or only Cu(I) oxide, is obtained as the percentage of water in the electrolyte solution decreases. A preliminary study was also carried out for the photocatalytic degradation of the methylene blue (MB) dye under irradiation with simulated sunlight in the presence of the nanoneedles obtained, presenting a maximum degradation value of 88% of MB and, thus, demonstrating the potential characteristics of the material investigated in the degradation of organic dyes.
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http://dx.doi.org/10.3390/nano11112994DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8621926PMC
November 2021

Fibrotic Remodeling during Persistent Atrial Fibrillation: In Silico Investigation of the Role of Calcium for Human Atrial Myofibroblast Electrophysiology.

Cells 2021 Oct 22;10(11). Epub 2021 Oct 22.

Institute of Biomedical Engineering, Karlsruhe Institute of Technology (KIT), 76131 Karlsruhe, Germany.

During atrial fibrillation, cardiac tissue undergoes different remodeling processes at different scales from the molecular level to the tissue level. One central player that contributes to both electrical and structural remodeling is the myofibroblast. Based on recent experimental evidence on myofibroblasts' ability to contract, we extended a biophysical myofibroblast model with Ca handling components and studied the effect on cellular and tissue electrophysiology. Using genetic algorithms, we fitted the myofibroblast model parameters to the existing in vitro data. In silico experiments showed that Ca currents can explain the experimentally observed variability regarding the myofibroblast resting membrane potential. The presence of an L-type Ca current can trigger automaticity in the myofibroblast with a cycle length of 799.9 ms. Myocyte action potentials were prolonged when coupled to myofibroblasts with Ca handling machinery. Different spatial myofibroblast distribution patterns increased the vulnerable window to induce arrhythmia from 12 ms in non-fibrotic tissue to 22 ± 2.5 ms and altered the reentry dynamics. Our findings suggest that Ca handling can considerably affect myofibroblast electrophysiology and alter the electrical propagation in atrial tissue composed of myocytes coupled with myofibroblasts. These findings can inform experimental validation experiments to further elucidate the role of myofibroblast Ca handling in atrial arrhythmogenesis.
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http://dx.doi.org/10.3390/cells10112852DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8616446PMC
October 2021

Identification of a Distinct miRNA Regulatory Network in the Tumor Microenvironment of Transformed Mycosis Fungoides.

Cancers (Basel) 2021 Nov 22;13(22). Epub 2021 Nov 22.

Division of Dermatology, City of Hope, Duarte, CA 91010, USA.

Large cell transformation of mycosis fungoides (LCT-MF) occurs in 20-50% of advanced MF and is generally associated with poor response and dismal prognosis. Although different mechanisms have been proposed to explain the pathogenesis, little is known about the role of microRNAs (miRs) in transcriptional regulation of LCT-MF. Here, we investigated the miR and mRNA expression profile in lesional skin samples of patients with LCT-MF and non-LCT MF using RNA-seq analysis. We found miR-146a and miR-21 to be significantly upregulated, and miR-708 the most significantly downregulated miR in LCT-MF. Integration of miR and mRNA expression profiles revealed the miR-regulated networks in LCT-MF. Ingenuity pathway analysis (IPA) demonstrated the involvement of genes for ICOS-ICOSL, PD1-PDL1, NF-κB, E2F transcription, and molecular mechanisms of cancer signaling pathways. Quantitative real time (qRT)-PCR results of target genes were consistent with the RNA-seq data. We further identified the immunosuppressive tumor microenvironment (TME) in LCT-MF. Moreover, our data indicated that miR-146a, -21 and -708 are associated with the immunosuppressive TME in LCT-MF. Collectively, our results suggest that the key LCT-MF associated miRs and their regulated networks may provide insights into its pathogenesis and identify promising targets for novel therapeutic strategies.
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http://dx.doi.org/10.3390/cancers13225854DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8616450PMC
November 2021
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