Publications by authors named "J L Moreno"

2,540 Publications

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An alternative, zeaxanthin epoxidase-independent abscisic acid biosynthetic pathway in plants.

Mol Plant 2021 Sep 18. Epub 2021 Sep 18.

Biological and Environmental Sciences and Engineering Division, Center for Desert Agriculture, The BioActives Lab, King Abdullah University of Science and Technology, Thuwal, 23955-6900, Saudi Arabia. Electronic address:

Abscisic acid (ABA) is an important carotenoid-derived phytohormone that plays essential roles in plant response to biotic and abiotic stresses as well as in various physiological and developmental processes. In Arabidopsis, ABA biosynthesis starts with the epoxidation of zeaxanthin by the ABA DEFICIENT 1 (ABA1) enzyme, leading to epoxycarotenoids, e.g., violaxanthin. The oxidative cleavage of 9-cis-epoxycarotenoids, a key regulatory step catalyzed by 9-CIS-EPOXYCAROTENOID DIOXYGENASE, forms xanthoxin that is converted in further reactions mediated by ABA DEFICIENT 2 (ABA2), ABA DEFICIENT 3 (ABA3), and ABSCISIC ALDEHYDE OXIDASE 3 (AAO3) into ABA. By combining genetic and biochemical approaches, we unravel here an ABA1-independent ABA biosynthetic pathway starting upstream of zeaxanthin. We identified the carotenoid cleavage products, i.e., apocarotenoids, β-apo-11-carotenal, 9-cis-β-apo-11-carotenal, 3-OH-β-apo-11-carotenal, and 9-cis-3-OH-β-apo-11-carotenal as intermediates of this ABA1-independent ABA biosynthetic pathway. Using labeled compounds, we showed that β-apo-11-carotenal, 9-cis-β-apo-11-carotenal, and 3-OH-β-apo-11-carotenal are successively converted into 9-cis-3-OH-β-apo-11-carotenal, xanthoxin, and finally into ABA in both Arabidopsis and rice. When applied to Arabidopsis, these β-apo-11-carotenoids exert ABA biological functions, such as maintaining seed dormancy and inducing the expression of ABA-responsive genes. Indeed, the transcriptomic analysis revealed a high overlap of differentially expressed genes regulated by β-apo-11-carotenoids and ABA, but also suggested that these compounds exert ABA-independent regulatory activities. Taken together, our study identifies a biological function for the common plant metabolites β-apo-11-carotenoids, extends our knowledge about ABA biosynthesis and provides new insights into plant apocarotenoid metabolic networks.
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http://dx.doi.org/10.1016/j.molp.2021.09.008DOI Listing
September 2021

Effects and experience with the Mézières method in people with low back pain: A mixed methods study.

J Back Musculoskelet Rehabil 2021 Sep 10. Epub 2021 Sep 10.

Trauma and Clinical Rehabilitation, Clínica Universidad de La Sabana, Chia, Colombia.

Background: Low back pain (LBP) is one of the most common reasons for visiting the doctor. The Mézières method (MM) emphasises body awareness and uses a global postural rehabilitation approach. It is used in the management of LBP, but its effectiveness has received limited formal evaluation.

Objective: To determine the effects of MM on quality of life, pain and functional disability in people with LBP and understand the patient's bodily experience during the MM intervention.

Methods: This single-blind randomised controlled trial with a mixed methods design will include 54 people with LBP aged 18 to 65 years. Participants will be randomised into two groups, one will receive MM and the other will receive a control intervention, administered through 10 treatment sessions. Participants will also construct a narrative to provide an understanding of their bodily experience.

Results: The assessed outcomes will include pain, back pain-related disability assessed using the Roland Morris Questionnaire, and quality of life related to health assessed using the SF12. Outcomes will be assessed at baseline, after the intervention and at a 6 weeks follow-up.
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http://dx.doi.org/10.3233/BMR-200344DOI Listing
September 2021

The Vaccination Cold War.

Hastings Cent Rep 2021 Sep;51(5):12-17

Surveying the early responses to the Covid-19 pandemic among nation states, one finds a veritable babel of responses, some predictable and some not. Would these results have been different half a century or more ago, when smallpox was eradicated and hopes were high that international cooperation would yield similar results for other infectious diseases? Is this a story about the stability provided by the bipolar postwar world, juxtaposed with the complex geopolitical repositioning that finally followed the collapse of the Soviet Union, or is that too rich an irony? A multipolar world may indeed be less prepared to cope with an international health crisis than a bipolar one. In any case, the patterns of global response are not only reminiscent of the Cold War era itself but also suggestive of a new vaccination cold war.
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http://dx.doi.org/10.1002/hast.1282DOI Listing
September 2021

Previous fire occurrence, but not fire recurrence, modulates the effect of charcoal and ash on soil C and N dynamics in Pinus pinaster Aiton forests.

Sci Total Environ 2021 Aug 26;802:149924. Epub 2021 Aug 26.

Departamento de Ciencias Ambientales, Universidad de Castilla-La Mancha, Campus Fábrica de Armas, E-45071 Toledo, Spain.

Understanding the effects of fire history on soil processes is key to characterise their resistance and resilience under future fire events. Wildfires produce pyrogenic carbonaceous material (PCM) that is incorporated into the soil, playing a critical role in the global carbon (C) cycle, but its interactions with soil processes are poorly understood. We evaluated if the previous occurrence of wildfires modulates the dynamic of soil C and nitrogen (N) and microbial community by soil ester linked fatty acids, after a new simulated low-medium intensity fire. Soils with a different fire history (none, one, two or three fires) were heat-shocked and amended with charcoal and/or ash derived from Pinus pinaster. Soil C and N mineralization rates were measured under controlled conditions, with burned soils showing lower values than unburned (without fire for more than sixty years). In general, no effects of fire recurrence were observed for any of the studied variables. Microbial biomass was lower in burned, with a clear dominance of Gram-positive bacteria in these soils. PCM amendments increased cumulative carbon dioxide (CO) production only in previously burned soils, especially when ash was added. This contrasted response to PCM between burned and unburned soils in CO production could be related to the effect of the previous wildfire history on soil microorganisms. In burned soils some microorganisms might have been adapted to the resulting conditions after a new fire event. Burned soils showed a significant positive priming effect after PCM amendment, mainly ash, probably due to an increased pH and phosphorous availability. Our results reveal the role of different PCMs as drivers of C and N mineralization processes in burned soils when a new fire occurs. This is relevant for improving models that evaluate the net impact of fire in C cycling and to reduce uncertainties under future changing fire regimes scenarios.
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http://dx.doi.org/10.1016/j.scitotenv.2021.149924DOI Listing
August 2021

A dicoordinate gold(I)-ethylene complex.

Chem Commun (Camb) 2021 Sep 14;57(73):9280-9283. Epub 2021 Sep 14.

Instituto de Investigaciones Químicas (IIQ), Departamento de Química Inorgánica and Centro de Innovación en Química Avanzada (ORFEO-CINQA), Consejo Superior de Investigaciones Científicas (CSIC) and University of Sevilla, Sevilla 41092, Spain.

The use of the exceptionally bulky tris-2-(4,4'-di--butylbiphenylyl)phosphine ligand allows the isolation and complete characterization of the first dicoordinate gold(I)-ethylene adduct, filling a missing fundamental piece on the organometallic chemistry of gold. Besides, the bonding situation of this species has been investigated by means of state-of-the-art Density Functional Theory (DFT) calculations indicating that π-backdonation plays a minor role compared with tricoordinate analogues.
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http://dx.doi.org/10.1039/d1cc02769gDOI Listing
September 2021
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