Publications by authors named "J K Ahiakpa"

7 Publications

COVID-19 vaccines uptake: Public knowledge, awareness, perception and acceptance among adult Africans.

PLoS One 2022 1;17(6):e0268230. Epub 2022 Jun 1.

Yaba College of Technology, Lagos, Nigeria.

Introduction: The willingness of Africa's population to patronise the COVID-19 vaccines is critical to the efficiency of national immunisation programmes. This study surveys the views of adult African inhabitants toward vaccination and the possibility of participating or not participating in governments' efforts to get citizens vaccinated.

Method: A cross-sectional online survey of adult Africans was undertaken from December 2020 to March 2021. Responses were anonymised. The Pearson Chi-square test was performed to determine whether or not there were any variations in knowledge, awareness, perception and acceptance of the COVID-19 vaccines among the participants. Binomial logistic regression was used to evaluate the factors associated with willingness to accept the COVID-19 vaccines and participate in immunisation programmes.

Results: The results indicate that COVID-19 vaccines are more likely to be used by adult Africans over the age of 18 who are largely technologically savvy (55 percent) if the vaccine is made broadly available. A total of 33 percent of those who responded said they were unlikely to receive the vaccine, with another 15 percent stating they were undecided. Aside from that, we found that vaccine hesitancy was closely associated with socio-demographic characteristics such as age, gender, education and source of information. We also found that there were widespread conspiracies and myths about the COVID-19 vaccines.

Conclusion: More than one-third of African adults who participated in the survey indicated they would not receive the COVID-19 vaccine, with majority of them expressing skepticisms about the vaccine's efficacy. It is possible that many of the people who would not be vaccinated would have an impact on the implementation of a COVID-19 immunisation programme that is meant for all of society. Majority of the respondents were unwilling to pay for the COVID-19 vaccines when made available. An awareness campaign should be focused on promoting the benefits of vaccination at the individual and population levels, as well as on taking preemptive actions to debunk misconceptions about the vaccines before they become further widespread.
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June 2022

Genome Sequence of an from Egypt.

Microbiol Resour Announc 2021 Jul 22;10(29):e0053321. Epub 2021 Jul 22.

Botany and Microbiology Department, Faculty of Science, Benha University, Benha, Egypt.

A complete DNA-A was sequenced from okra in Egypt. Here, we report the complete genome sequence of this monopartite virus, comprising 2,764 bp and encoding 6 open reading frames (ORFs) with a GC content of 44.6% and 88.3% similarity to a virus reported earlier from Cameroon.
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July 2021

Influence of plastic film mulch with biochar application on crop yield, evapotranspiration, and water use efficiency in northern China: A meta-analysis.

PeerJ 2021 3;9:e10967. Epub 2021 Mar 3.

Research Desk Consulting Ltd, Accra, Ghana.

Background: China is the leading consumer of plastic film worldwide. Plastic film mulched ridge-furrow is one of the most widely adopted agronomic and field management practices in rain-fed agriculture in dry-land areas of China. The efficiency of plastic film mulching as a viable method to decrease evapotranspiration (ET), increase crop yields, and water use efficiency (WUE), has been demonstrated extensively by earlier studies.

Methods: A comprehensive evaluation of how co-application of plastic-film mulch and biochar in different agro-environments under varying climatic conditions influence ET, crop yield, WUE, and soil microbial activity were assessed. We performed a meta-analysis using the PRISMA guideline to assess the effect of plastic-film mulched ridge-furrow and biochar on ET, yield, and WUE of wheat ( L.), potato ( L.), and maize ( L.) in northern China.

Results: The use of plastic film increased average yields of wheat (75.7%), potato (20.2%), and maize (12.9%) in Gansu, Ningxia, Shaanxi, and Shanxi provinces, respectively due to the reduction in ET by 12.8% in Gansu, 0.5% in Ningxia, and 4.1% in Shanxi, but increased in Shaanxi by 0.5% compared to no-mulching. These changes may be attributed to the effect of plastic film mulch application which simultaneously increased WUE by 68.5% in Gansu, 23.9% in Ningxia, 16.2% in Shaanxi, and 12.8% in Shanxi, respectively. Compared to flat planting without mulching, in three years, the yield of maize increased with the co-application of plastic film and biochar by 22.86% in the Shanxi and Shaanxi regions.

Conclusion: Our analysis revealed co-application of plastic film with biochar is integral for improving soil and water conservation in rain-fed agriculture and as an integrated practice to avert drought while simultaneously mitigating runoff and erosion.
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March 2021

Genome-Wide Identification and Molecular Characterization of the Growth-Regulating Factors-Interacting Factor Gene Family in Tomato.

Genes (Basel) 2020 11 28;11(12). Epub 2020 Nov 28.

Key Laboratory of Horticultural Plant Biology, Ministry of Education, Huazhong Agricultural University, 430070 Wuhan, China.

Growth-regulating factors-interacting factor (GIF) proteins play crucial roles in the regulation of plant growth and development. However, the molecular mechanism of GIF proteins in tomato is poorly understood. Here, four genes (named , , , and ) were identified from the tomato genome and clustered into two major clades by phylogenetic analysis. The gene structure and motif pattern analyses showed similar exon/intron patterns and motif organizations in all the . We identified 33 -acting regulatory elements (CAREs) in the promoter regions of the . The expression profiling revealed the four are expressed in various tissues and stages of fruit development and induced by phytohormones (IAA and GA). The subcellular localization assays showed all four GIFs were located in nucleus. The yeast two-hybrid assay indicated various growth-regulating factors (SlGRFs) proteins interacted with the four SlGIF proteins. However, SlGRF4 was a common interactor with the SlGIF proteins. Moreover, a higher co-expression relationship was shown between three genes and five genes. The protein association network analysis found a chromodomain helicase DNA-binding protein (CHD) and an actin-like protein to be associated with the four SlGIF proteins. Overall, these results will improve our understanding of the potential functions of genes and act as a base for further functional studies on in tomato growth and development.
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November 2020

Genome-wide analysis of Myo-inositol oxygenase gene family in tomato reveals their involvement in ascorbic acid accumulation.

BMC Genomics 2020 Apr 6;21(1):284. Epub 2020 Apr 6.

Key Laboratory of Horticultural Plant Biology, Ministry of Education, Huazhong Agricultural University, Wuhan, 430070, China.

Background: Ascorbic acid (Vitamin C, AsA) is an antioxidant metabolite involved in plant development and environmental stimuli. AsA biosynthesis has been well studied in plants, and MIOX is a critical enzyme in plants AsA biosynthesis pathway. However, Myo-inositol oxygenase (MIOX) gene family members and their involvement in AsA biosynthesis and response to abiotic stress remain unclear.

Results: In this study, five tomato genes encoding MIOX proteins and possessing MIOX motifs were identified. Structural analysis and distribution mapping showed that 5 MIOX genes contain different intron/exon patterns and unevenly distributed among four chromosomes. Besides, expression analyses indicated the remarkable expression of SlMIOX genes in different plant tissues. Furthermore, transgenic lines were obtained by over-expression of the MIOX4 gene in tomato. The overexpression lines showed a significant increase in total ascorbate in leaves and red fruits compared to control. Expression analysis revealed that increased accumulation of AsA in MIOX4 overexpression lines is possible as a consequence of the multiple genes involved in AsA biosynthesis. Myo inositol (MI) feeding in leaf and fruit implied that the Myo-inositol pathway improved the AsA biosynthesis in leaves and fruits. MIOX4 overexpression lines exhibited a better light response, abiotic stress tolerance, and AsA biosynthesis capacity.

Conclusions: These results showed that MIOX4 transgenic lines contribute to AsA biosynthesis, evident as better light response and improved oxidative stress tolerance. This study provides the first comprehensive analysis of the MIOX gene family and their involvement in ascorbate biosynthesis in tomato.
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April 2020