Publications by authors named "J Hu"

22,836 Publications

The pattern of duck sternal ossification and the changes of histological structure and gene expression therein.

Poult Sci 2021 Mar 12;100(7):101112. Epub 2021 Mar 12.

Farm Animal Genetic Resources Exploration and Innovation Key Laboratory of Sichuan Province, College of Animal Science and Technology, Sichuan Agricultural University, Chengdu, Sichuan 611130, China.

As the largest single bone, avian sterna are very different from those of mammals in terms of morphology and functions. Moreover, years of artificial selection in poultry led to incomplete sternal ossification at slaughter age, which may cause diseases, sternal injury, and restriction to breast muscle growth. However, in living birds, studies have rarely described the ossification pattern and underlying mechanisms of the sterna. Here, we examined the pattern (timeline, ossification centers, ossification directions, weekly changes of different parts, quantified differences in ossification degree among sexes and parts) and developmental changes (histological structure, gene expression) of postnatal duck sternal ossification. Direct observation and alcian blue and alizarin red staining of whole sterna samples revealed that, duck sterna mainly ossified during 5 to 9 wk old with five ossification centers. These centers and their ossification directions were different from and more complex than the previously studied birds. The weekly changes of sterna and the quantitative analysis of ossification-related traits showed that ossifications in the three parts of duck sterna (sternum body, keel, posterolateral processes) were mutually independent in space and time, meanwhile, the male duck sterna were more late-maturing than the female. The results of hematoxylin-eosin, alcian blue, and toluidine blue stainings and the expression levels of COL2A1, COL10A1, COL1A2, and CTSK together supported that, duck sternal ossification was highly similar to typical endochondral ossification. Furthermore, continuously high expression of MMP13 and SPARC and their significant (P < 0.05) co-expression with COL2A1, COL10A1, COL1A2, and CTSK suggested the importance of MMP13 and SPARC in duck sternal ossification. Taken together, our results may be helpful for the understanding of avian sternal ossification and the improvement of the performance and welfare of poultry from a new perspective.
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http://dx.doi.org/10.1016/j.psj.2021.101112DOI Listing
March 2021

DYF-4 regulates patched-related/DAF-6-mediated sensory compartment formation in C. elegans.

PLoS Genet 2021 Jun 11;17(6):e1009618. Epub 2021 Jun 11.

Center for Energy Metabolism and Reproduction, Institute of Biomedicine and Biotechnology, Shenzhen Institute of Advanced Technology, Chinese Academy of Sciences (CAS), Shenzhen, China.

Coordination of neurite extension with surrounding glia development is critical for neuronal function, but the underlying molecular mechanisms remain poorly understood. Through a genome-wide mutagenesis screen in C. elegans, we identified dyf-4 and daf-6 as two mutants sharing similar defects in dendrite extension. DAF-6 encodes a glia-specific patched-related membrane protein that plays vital roles in glial morphogenesis. We cloned dyf-4 and found that DYF-4 encodes a glia-secreted protein. Further investigations revealed that DYF-4 interacts with DAF-6 and functions in a same pathway as DAF-6 to regulate sensory compartment formation. Furthermore, we demonstrated that reported glial suppressors of daf-6 could also restore dendrite elongation and ciliogenesis in both dyf-4 and daf-6 mutants. Collectively, our data reveal that DYF-4 is a regulator for DAF-6 which promotes the proper formation of the glial channel and indirectly affects neurite extension and ciliogenesis.
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http://dx.doi.org/10.1371/journal.pgen.1009618DOI Listing
June 2021

Plant Hormones and Volatiles Response to Temperature Stress in Sweet Corn ( L.) Seedlings.

J Agric Food Chem 2021 Jun 11. Epub 2021 Jun 11.

School of Food Science and Engineering, South China University of Technology, Guangzhou 510640, China.

This work aims to emphasize on disclosing the regulative mechanism of sweet corn seedlings response to extreme temperature stress; transcriptomics and metabolomics for volatiles and plant hormones were integrated in this study. Results showed that low-temperature stress significantly impressed 20 volatiles; abscisic acid and salicylic acid accumulated, while auxin and jasmonic acid decreased. The regulatory patterns of and for abscisic acid accumulation and signal transduction were elucidated in low-temperature stress. High-temperature stress influenced 31 volatiles and caused the reductions on zeatin, salicylic acid, jasmonic acid, and auxin. The up-regulation of an gene emphasized its function on zeatin signal transduction under high-temperature stress. Correlations among gene modules, phytohormones, and volatiles were analyzed for building the regulative network of sweet corn seedlings under temperature stress. The attained result might build foundations for improving early development of sweet corn by biological intervention or genomic-level modulation.
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http://dx.doi.org/10.1021/acs.jafc.1c02275DOI Listing
June 2021

Association of smoking with postoperative atrial fibrillation in patients with cardiac surgery: A PRISMA-compliant article.

Medicine (Baltimore) 2021 Jun;100(23):e26179

Jiangxi Provincial People's Hospital Affiliated to Nanchang University, Nanchang of Jiangxi.

Background: Cigarette smoking is an important modifiable risk factor for incident atrial fibrillation. However, the impact of smoking on postoperative atrial fibrillation in patients undergoing cardiac surgery remains controversial. We performed this meta-analysis to explore the association of smoking with postoperative atrial fibrillation in patients with cardiac surgery.

Methods: We systematically searched 2 computer-based databases (PubMed and EMBASE) up to July 2019 for all relevant studies. A random-effects model was selected to pool the odds ratios (ORs) and 95% confidence intervals (CIs). In this meta-analysis, the protocol and reporting of the results were based on the Preferred Reporting Items for Systematic reviews and Meta-Analyses (PRISMA) statement.

Results: A total of 36 studies were included in this meta-analysis. Overall, smoking was not associated with an increased risk of postoperative atrial fibrillation in patients undergoing cardiac surgery (odds ratio [OR] = 0.89; 95% confidence interval [CI] 0.79-1.02). The corresponding results were stable in the subgroup analyses. Specifically, smoking was not associated with an increased risk of postoperative atrial fibrillation regardless of the type of cardiac surgery: coronary artery bypass grafting (OR = 0.91; 95% CI 0.77-1.07), valve surgery (OR = 0.15; 95% CI 0.01-1.56), and coronary artery bypass grafting+valve surgery (OR = 0.91; 95% CI 0.70-1.18).

Conclusions: Based on currently published studies, smoking was not associated with an increased risk of postoperative atrial fibrillation in patients undergoing cardiac surgery.
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http://dx.doi.org/10.1097/MD.0000000000026179DOI Listing
June 2021

mTORC2 regulates hierarchical micro/nano topography-induced osteogenic differentiation via promoting cell adhesion and cytoskeletal polymerization.

J Cell Mol Med 2021 Jun 10. Epub 2021 Jun 10.

Department of Prosthodontics, Tianjin Medical University School and Hospital of Stomatology, Tianjin, China.

Surface topography acts as an irreplaceable role in the long-term success of intraosseous implants. In this study, we prepared the hierarchical micro/nano topography using selective laser melting combined with alkali heat treatment (SLM-AHT) and explored the underlying mechanism of SLM-AHT surface-elicited osteogenesis. Our results show that cells cultured on SLM-AHT surface possess the largest number of mature FAs and exhibit a cytoskeleton reorganization compared with control groups. SLM-AHT surface could also significantly upregulate the expression of the cell adhesion-related molecule p-FAK, the osteogenic differentiation-related molecules RUNX2 and OCN as well as the mTORC2 signalling pathway key molecule Rictor. Notably, after the knocked-down of Rictor, there were no longer significant differences in the gene expression levels of the cell adhesion-related molecules and osteogenic differentiation-related molecules among the three titanium surfaces, and the cells on SLM-AHT surface failed to trigger cytoskeleton reorganization. In conclusion, the results suggest that mTORC2 can regulate the hierarchical micro/nano topography-mediated osteogenesis via cell adhesion and cytoskeletal reorganization.
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http://dx.doi.org/10.1111/jcmm.16672DOI Listing
June 2021