Neurology 2021 Apr 1. Epub 2021 Apr 1.
Gregory S Day: Department of Neurology, Mayo Clinic, Jacksonville, Florida, United States of America Melanie Y Yarbrough: Department of Pathology and Immunology; Washington University School of Medicine, St. Louis, Missouri, United States of AmericaPeter Körtvelyessy: Department of Neurology; University of Magdeburg, Magdeburg, Germany; and Department of Neurology and Experimental Neurology; Charité, Universitätmedizin Berlin, Germany Harald Prüss: Department of Neurology and Experimental Neurology; Charité, Universitätmedizin Berlin, GermanyRobert C Bucelli: Department of Neurology; Washington University School of Medicine, Saint Louis, Missouri, United States of America Marvin J Fritzler: Department of Medicine; Cumming School of Medicine, University of Calgary, Calgary, Alberta, CanadaWarren Mason: Department of Medicine; Division of Neurology, University of Toronto, Toronto, Ontario David F Tang-Wai: Department of Medicine; Division of Neurology, University of Toronto, Toronto, OntarioClaude Steriade: NYU Langone Comprehensive Epilepsy Center; NYU Langone Health, New York City, New York, United States of America Julien Hébert: Department of Medicine; Division of Neurology, University of Toronto, Toronto, OntarioRachel L Henson: Department of Neurology; Washington University School of Medicine, Saint Louis, Missouri, United States of America; and The Charles F. and Joanne Knight Alzheimer Disease Research Center; Washington University School of Medicine, Saint Louis, Missouri, United States of America Elizabeth M Herries: Departments of Pathology and Immunology, and Neurology; Washington University School of Medicine, St. Louis, Missouri, United States of AmericaJack H Ladenson: Departments of Pathology and Immunology, and Neurology; Washington University School of Medicine, St. Louis, Missouri, United States of America A Sebastian Chiriboga-Lopez: Department of Neurology, Mayo Clinic, Jacksonville, Florida, United States of AmericaNeill R Graff-Radford: Department of Neurology, Mayo Clinic, Jacksonville, Florida, United States of America John C Morris: Department of Neurology; Washington University School of Medicine, Saint Louis, Missouri, United States of America; and The Charles F. and Joanne Knight Alzheimer Disease Research Center; Washington University School of Medicine, Saint Louis, Missouri, United States of AmericaAnne M Fagan: Department of Neurology; Washington University School of Medicine, Saint Louis, Missouri, United States of America; and The Charles F. and Joanne Knight Alzheimer Disease Research Center; Washington University School of Medicine, Saint Louis, Missouri, United States of America.
Objectives: To determine whether neuronal and neuroaxonal injury, neuroinflammation and synaptic dysfunction associate with clinical course and outcomes in antibody-mediated encephalitis (AME), we measured biomarkers of these processes in CSF from patients presenting with AME and cognitively normal individuals.
Methods: Biomarkers of neuronal (total-tau, VILIP-1) and neuroaxonal damage (neurofilament light chain [NfL]), inflammation (YKL-40) and synaptic function (neurogranin, SNAP-25) were measured in CSF obtained from 45 patients at the time of diagnosis of NMDA receptor (n=34) or LGI1/CASPR-2 (n=11) AME, and 39 age- and sex-similar cognitively normal individuals. The association between biomarkers and modified Rankin Scores were evaluated in a subset (n=20) of longitudinally followed patients.
Results: Biomarkers of neuroaxonal injury (NfL) and neuroinflammation (YKL-40) were elevated in AME cases at presentation, while markers of neuronal injury and synaptic function were stable (total-tau) or decreased (VILIP-1, SNAP-25, neurogranin). The log-transformed ratio of YKL-40/SNAP-25 optimally discriminated cases from cognitively normal individuals (AUC=0.99; 95%CI: 0.97, >0.99). Younger age (ρ=-0.56; p=0.01), lower VILIP-1 (ρ=-0.60; p<0.01) and SNAP-25 (ρ=-0.54; p=0.01), and higher log(YKL-40/SNAP-25) [(ρ=0.48; p=0.04] associated with greater disease severity (higher modified Rankin Score) in prospectively followed patients. Higher YKL-40 (ρ=0.60; p=0.02) and neurogranin (ρ=0.55; p=0.03) at presentation were associated with higher modified Rankin Scores 12-months following hospital discharge.
Conclusions: CSF biomarkers suggest that neuronal integrity is acutely maintained in AME patients, despite neuroaxonal compromise. Low-levels of biomarkers of synaptic function may reflect antibody-mediated internalization of cell-surface receptors, and may represent an acute correlate of antibody-mediated synaptic dysfunction, with the potential to inform disease severity and outcomes.