Publications by authors named "Izabela Nowak"

77 Publications

Catalytic role of metals supported on SBA-16 in hydrodeoxygenation of chemical compounds derived from biomass processing.

RSC Adv 2021 Mar 3;11(16):9505-9517. Epub 2021 Mar 3.

Adam Mickiewicz University, Poznań, Faculty of Chemistry Uniwersytetu Poznańskiego 8 61-614 Poznań Poland

Hydrodeoxygenation (HDO) carried out at high temperatures and high hydrogen pressures is one of the alternative methods of upgrading pyrolytic oils from biomass, leading to high quality biofuels. To save energy, it is important to carry out catalytic processes under the mildest possible experimental conditions. The aim of our research was the synthesis of ordered mesoporous SBA-16 type silica materials modified with transition metal atoms (Ir, Ru, Pd, Pt), their physicochemical characterization and use as catalysts in hydrodeoxygenation of model chemicals (guaiacol, syringol, creosol). The HDO process was carried out under mild experimental conditions at temperatures in the range from 90 to 130 °C and hydrogen pressures in the range from 25 to 60 bar. The catalytic tests revealed differences in the catalytic properties of the samples studied. The catalytic systems used assured highly efficient transformations of the examined molecules as well as high selectivity towards chemical compounds with lower O/C ratio and higher H/C ratio as compared to those in the initial substrates. High activity of the catalysts containing precious metals in the experimental conditions applied suggests their potential to improve bio-oil production for biofuels.
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http://dx.doi.org/10.1039/d0ra06696fDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8695449PMC
March 2021

Optimization of the Conditions of Solid Lipid Nanoparticles (SLN) Synthesis.

Molecules 2022 Mar 28;27(7). Epub 2022 Mar 28.

Faculty of Chemistry, Adam Mickiewicz University, 8: Uniwersytetu Poznańskiego, 61-614 Poznan, Poland.

Solid lipid nanoparticles (SLNs) have been synthesized as potential drug delivery systems. They are classified as solid lipid nanocarriers that can successfully carry both hydrophilic and hydrophobic drugs. SLNs are based on a biocompatible lipid matrix that is enzymatically degraded into natural components found in the human body. Solid lipid nanoparticles are suitable for the incorporation of hydrophobic active ingredients such as curcumin. The study included the optimization of lipid nanoparticle composition, incorporation of the active compound (curcumin), a stability evaluation of the obtained nanocarriers and characterization of their lipid matrix. Through process optimization, a dispersion of solid lipid nanoparticles (solid lipid:surfactant-2:1.25 weight ratio) predisposed to the incorporation of curcumin was developed. The encapsulation efficiency of the active ingredient was determined to be 99.80%. In stability studies, it was found that the most suitable conditions for conducting high-pressure homogenization are 300 bar pressure, three cycles and a closed-loop system. This yields the required values of the physicochemical parameters (a particle size within a 200-450 nm range; a polydispersity index of <30%; and a zeta potential of about |±30 mV|). In this work, closed-loop high-pressure homogenization was used for the first time and compared to the currently preferred open-loop method.
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http://dx.doi.org/10.3390/molecules27072202DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9000502PMC
March 2022

The Association of Gene Polymorphism and Its Soluble Form With Male Infertility.

Front Immunol 2021 17;12:791399. Epub 2022 Jan 17.

Department of Clinical Immunology, Laboratory of Immunogenetics and Tissue Immunology, Ludwik Hirszfeld Institute of Immunology and Experimental Therapy, Polish Academy of Sciences, Wrocław, Poland.

Successful reproduction depends on many factors. Male factors contribute to infertility in approximately 50% of couples who fail to conceive. Seminal plasma consists of secretions from different accessory glands containing a mixture of various cytokines, chemokines, and growth factors, which together can induce a local immune response that might impact on a male's as well as a female's fertility. Human leukocyte antigen (HLA)-G expression has been suggested as an immunomodulatory molecule that influences pregnancy outcome. The gene encodes either membrane-bound or/and soluble proteins. The aim of this study was the evaluation of HLA-G polymorphisms and their impact on soluble HLA-G (sHLA-G) production. We tested the polymorphism in three positions: rs1632947: c.-964G>A; rs1233334: c.-725G>C/T in the promoter region; rs371194629: c.∗65_∗66insATTTGTTCATGCCT in the 3' untranslated region. We tested two cohorts of men: 663 who participated in fertilization (test material was blood or sperm), and 320 fertile controls who possessed children born after natural conception (test material was blood). Since 50% of men visiting assisted reproductive clinics have abnormal semen parameters, we wondered if men with normal sperm parameters differ from those with abnormal parameters in terms of polymorphism and secretion of sHLA-G into semen. We found that certain rs1632947-rs1233334-rs371194629 haplotypes and diplotypes were associated with male infertility, while others were protective. Normozoospermic men with the A-C-del haplotype and A-C-del/A-C-del diplotype secreted the most sHLA-G into semen (574.1 IU/mL and 1047.0 IU/mL, respectively), while those with the G-C-ins haplotype and G-C-ins/G-C-ins diplotype - the least (80.8 IU/mL and 75.7 IU/mL, respectively). Men with the remaining haplotypes/diplotypes secreted sHLA-G at an intermediate level. However, only in one haplotype, namely G-C-ins, did we observe strong significant differences in the concentration of sHLA-G in the semen of men with teratozoospermia compared to men with normal sperm parameters (p = 0.009). In conclusion, fertile men differ in the profile of polymorphism from men participating in IVF. Among all haplotypes, the most unfavorable for male fertility is the G-C-ins haplotype, which determines the secretion of the lowest concentration of the soluble HLA-G molecule. This haplotype may reduce sperm parameters.
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http://dx.doi.org/10.3389/fimmu.2021.791399DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8801424PMC
February 2022

Synthesis and Potential Applications of Lipid Nanoparticles in Medicine.

Materials (Basel) 2022 Jan 17;15(2). Epub 2022 Jan 17.

Faculty of Chemistry, Adam Mickiewicz University in Poznań, Uniwersytetu Poznańskiego 8, 61-614 Poznań, Poland.

Currently, carriers of active ingredients in the form of particles of a size measured in nanometers are the focus of interest of research centers worldwide. So far, submicrometer emulsions, liposomes, as well as microspheres, and nanospheres made of biodegradable polymers have been used in medicine. Recent studies show particular interest in nanoparticles based on lipids, and at the present time, are even referred to as the "era of lipid carriers". With the passage of time, lipid nanoparticles of the so-called first and second generation, SLN (Solid Lipid Nanoparticles) and nanostructured lipid carriers and NLC (Nanostructured Lipid Carriers), respectively, turned out to be an alternative for all imperfections of earlier carriers. These carriers are characterized by a number of beneficial functional properties, including, among others, structure based on lipids well tolerated by the human body, high stability, and ability to carry hydro- and lipophilic compounds. Additionally, these carriers can enhance the distribution of the drug in the target organ and alter the pharmacokinetic properties of the drug carriers to enhance the medical effect and minimize adverse side effects. This work is focused on the current review of the state-of-the-art related to the synthesis and applications of popular nanoparticles in medicine, with a focus on their use, e.g., in COVID-19 vaccines.
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http://dx.doi.org/10.3390/ma15020682DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8780297PMC
January 2022

Adiponectin Gene Polymorphism (rs17300539) Has No Influence on the Occurrence of Metabolic Syndrome in Women with Polycystic Ovary Syndrome.

Genes (Basel) 2021 11 27;12(12). Epub 2021 Nov 27.

Department of Gynecology, Endocrinology and Gynecological Oncology, Pomeranian Medical University in Szczecin, 71-256 Szczecin, Poland.

Adiponectin (rs17300539) is implicated in the pathogenesis of metabolic syndrome (MS), a common comorbidity of polycystic ovarian syndrome (PCOS). The aim of this study was to analyze the association between adiponectin gene polymorphism and incidence of MS in patients with PCOS. The study included 201 women (age 18 to 35 years), among them 81 patients with PCOS without concomitant MS, 70 subjects with PCOS and concomitant, and 50 regularly menstruating controls. Adiponectin gene polymorphism (11391 G/A, rs17300539) was determined by means of a real-time PCR. The study groups did not differ significantly in terms of their age and frequencies of various genotypes of the adiponectin gene polymorphism. The largest proportion in the whole group was Caucasian women ( = 178, 88.56%), who carried the GG genotype of the polymorphism; frequencies of GA and AA genotypes in the whole study group were 10.94% ( = 22) and 0.5% ( = 1), respectively. The presence of G or A allele of the rs17300539 adiponectin gene polymorphism was not associated with a greater likelihood of PCOS with/without concomitant MS. The hereby presented findings imply that MS is a common comorbidity in women with PCOS. However, the incidence of concomitant MS does not seem to be associated with adiponectin gene polymorphism.
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http://dx.doi.org/10.3390/genes12121902DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8701950PMC
November 2021

ERAP, KIR, and HLA-C Profile in Recurrent Implantation Failure.

Front Immunol 2021 22;12:755624. Epub 2021 Oct 22.

Laboratory of Immunogenetics and Tissue Immunology, Department of Clinical Immunology, Ludwik Hirszfeld Institute of Immunology and Experimental Therapy, Polish Academy of Sciences, Wrocław, Poland.

The mother's uterine immune system is dominated by uterine natural killer (NK) cells during the first trimester of pregnancy. These cells express killer cell immunoglobulin-like receptors (KIRs) of inhibitory or activating function. Invading extravillous trophoblast cells express HLA-C molecules, and both maternal and paternal HLA-C allotypes are presented to KIRs. Endoplasmic reticulum aminopeptidase 1 (ERAP1) and 2 (ERAP2) shape the HLA class I immunopeptidome. The ERAPs remove N-terminal residues from antigenic precursor peptides and generate optimal-length peptides to fit into the HLA class I groove. The inability to form the correct HLA class I complexes with the appropriate peptides may result in a lack of immune response by NK cells. The aim of this study was to investigate the role of and polymorphisms in the context of and genes in recurrent implantation failure (RIF). In addition, for the first time, we showed the results of ERAP1 and ERAP2 secretion into the peripheral blood of patients and fertile women. We tested a total of 881 women. Four hundred ninety-six females were patients who, together with their partners, participated in fertilization (IVF). A group of 385 fertile women constituted the control group. Women positive for genes in the Tel AA region and were more prevalent in the RIF group than in fertile women (p/p = 0.004/0.012, OR = 2.321). Of the polymorphisms studied, two of them (rs26653 and rs26618) appear to affect RIF susceptibility in HLA-C2-positive patients. Moreover, fertile women who gave birth in the past secreted significantly more ERAP1 than IVF women and control pregnant women (p < 0.0001 and p = 0.0005, respectively). In the case of ERAP2, the opposite result was observed; i.e., fertile women secreted far less ERAP2 than IVF patients (p = 0.0098). Patients who became pregnant after fertilization embryo transfer (IVF-ET) released far less ERAP2 than patients who miscarried (p = 0.0032). Receiver operating characteristic (ROC) analyses indicate a value of about 2.9 ng/ml of ERAP2 as a point of differentiation between patients who miscarried and those who gave birth to a healthy child. Our study indicates that both ERAP1 and ERAP2 may be involved in processes related to reproduction.
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http://dx.doi.org/10.3389/fimmu.2021.755624DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8569704PMC
February 2022

Surface Studies on Glass Powders Used in Commercial Glass-Ionomer Dental Cements.

Molecules 2021 Aug 31;26(17). Epub 2021 Aug 31.

Department of Biomaterials and Experimental Dentistry, Poznan University of Medical Sciences, Bukowska 70, 60-812 Poznań, Poland.

The surface properties of three commercial ionomer glass powders, i.e., Fuji IX, Kavitan Plus and Chemadent G-J-W were studied. Samples were analyzed by X-ray fluorescence spectroscopy (XRF), and the density was determined by gas pycnometry. Morphology was studied using scanning electron microscopy (SEM) and laser diffraction (LD) technique, whereas low-temperature nitrogen sorption measurements determined textural parameters like specific surface area and pore volume. Thermal transformations in the materials studied were evaluated by thermogravimetric analysis (TGA), which was carried out in an inert atmosphere between 30 °C and 900 °C. XRF showed that Fuji IX and Kavitan Plus powders were strontium-based, whereas Chemadent G-J-W powder was calcium-based. Powders all had a wide range of particle sizes under SEM and LD measurements. Specific surface areas and pore volumes were in the range 1.42-2.73 m/g and 0.0029 to 0.0083 cm/g, respectively, whereas densities were in the range 2.6428-2.8362 g/cm. Thermogravimetric analysis showed that the glass powders lost mass in a series of steps, with Fuji IX powder showing the highest number, some of which are attributed to the dehydration and decomposition of the polyacrylic acid present in this powder. Mass losses were more straightforward for the other two glasses. All three powders showed distinct losses at around 780 °C and 835 °C, suggesting that similar dehydration steps occur in all these glasses. Other steps, which differed between glass powders, are attributed to variations in states of water-binding on their surfaces.
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http://dx.doi.org/10.3390/molecules26175279DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8433982PMC
August 2021

Lipid Nanoparticles Loaded with Selected Iridoid Glycosides as Effective Components of Hydrogel Formulations.

Materials (Basel) 2021 Jul 22;14(15). Epub 2021 Jul 22.

Department of Applied Chemistry, Faculty of Chemistry, Adam Mickiewicz University, Uniwersytetu Poznańskiego 8, 61-614 Poznan, Poland.

One possibility of improving active ingredient penetration into deeper skin layers to enhance the cosmetic product effectiveness, is the application of lipid nanoparticles. The aim of the study presented in this paper was to evaluate the potential of hydrogel formulations enriched with iridoid glycosides-loaded lipid nanoparticles. Lipid nanocarriers were produced using an emulsification-ultrasonication method based on multiple emulsions. The encapsulation efficiency was determined at the level of 89% and 77% for aucubin and catalpol, respectively. The next stage was the incorporation of the obtained dispersions of lipid nanoparticles into hydrogel formulations, followed by determination of their physicochemical properties, shelf-life stability, and application properties (in vivo tests). The introduction of lipid nanoparticles increased the stabilization of the consistency of the obtained hydrogel formulations, and was confirmed by viscosity measurements. No effect of lipid nanoparticle incorporation on shelf-life stability of the hydrogels was detected. In vivo studies showed improvements in moisture content of the epidermis, transepidermal water loss, skin topography, and macrorelief parameters. In particular, a synergistic effect of the active ingredients and lipid nanoparticles on the anti-wrinkle effect, moisturizing effect, and regeneration of the protective barrier of the stratum corneum was evidenced. The attractiveness of aucubin and catalpol as cosmetic raw materials in hydrogel formulations was evidenced, especially when the iridoid glycosides were applied in the form of lipid nanoparticles.
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http://dx.doi.org/10.3390/ma14154090DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8347055PMC
July 2021

Diatom Biosilica Doped with Palladium(II) Chloride Nanoparticles as New Efficient Photocatalysts for Methyl Orange Degradation.

Int J Mol Sci 2021 Jun 23;22(13). Epub 2021 Jun 23.

Faculty of Chemistry, Adam Mickiewicz University in Poznań, Uniwersytetu Poznańskiego 8, 61-614 Poznań, Poland.

A new catalyst based on biosilica doped with palladium(II) chloride nanoparticles was prepared and tested for efficient degradation of methyl orange (MO) in water solution under UV light excitation. The obtained photocatalyst was characterized by X-ray diffraction, TEM and N adsorption/desorption isotherms. The photocatalytic degradation process was studied as a function of pH of the solution, temperature, UV irradiation time, and MO initial concentration. The possibilities of recycling and durability of the prepared photocatalysts were also tested. Products of photocatalytic degradation were identified by liquid chromatography-mass spectrometry analyses. The photocatalyst exhibited excellent photodegradation activity toward MO degradation under UV light irradiation. Rapid photocatalytic degradation was found to take place within one minute with an efficiency of 85% reaching over 98% after 75 min. The proposed mechanism of photodegradation is based on the assumption that both HO and O radicals, as strongly oxidizing species that can participate in the dye degradation reaction, are generated by the attacks of photons emitted from diatom biosilica (photonic scattering effect) under the influence of UV light excitation. The degradation efficiency significantly increases as the intensity of photons emitted from biosilica is enhanced by palladium(II) chloride nanoparticles immobilized on biosilica (synergetic photonic scattering effect).
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http://dx.doi.org/10.3390/ijms22136734DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8267799PMC
June 2021

β-cyclodextrin-containing polymer based on renewable cellulose resources for effective removal of ionic and non-ionic toxic organic pollutants from water.

J Hazard Mater 2021 09 1;418:126286. Epub 2021 Jun 1.

Department of Applied Chemistry, Faculty of Chemistry, Adam Mickiewicz University, 8 Uniwersytetu Poznańskiego Street, 61-614 Poznań, Poland.

A novel, bio-derived cyclodextrin-based trifunctional adsorbent has been successfully synthesized for efficient, rapid and simultaneous removal of a broad-spectrum of toxic ionic (anionic and cationic dyes) and non-ionic organic pollutants from water. The composition, morphology and the presence of functional groups in the obtained sorption material were characterized by elemental analysis, XRD, SEM, and FTIR spectroscopy. The adsorption results were represented by cationic dye (crystal violet, CV) and endocrine disrupting compound (bisphenol A, BPA) as an adsorbate. The sorption processes of the model pollutants were studied with both kinetic and equilibrium models. The results showed that the sorption was rapid (less than 1 min) and the time evolution could be fitted using a pseudo-second order model. According to Langmuir isotherm model, the maximum adsorption capacities were found at 113.64 and 43.10 mg g for BPA and CV, respectively. The adsorption ability of β-CDPs was kept nearly on the same level after five regeneration cycles. Furthermore, almost complete removal of the pollutants was observed during the treatment of real effluents samples thus the bio-derived, cheap and reusable BAN-EPI-CDP has a promising potential for practical applications.
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http://dx.doi.org/10.1016/j.jhazmat.2021.126286DOI Listing
September 2021

Lipid Nanoparticles Loaded with Iridoid Glycosides: Development and Optimization Using Experimental Factorial Design.

Molecules 2021 May 25;26(11). Epub 2021 May 25.

Department of Applied Chemistry, Faculty of Chemistry, Adam Mickiewicz University in Poznań, Uniwersytetu Poznańskiego 8, 61-614 Poznan, Poland.

Lipid nanoparticles based on multiple emulsion (W/O/W) systems are suitable for incorporating hydrophilic active substances, including iridoid glycosides. This study involved optimization of composition of lipid nanoparticles, incorporation of active compounds (aucubin and catalpol), evaluation of stability of the resulting nanocarriers, and characterization of their lipid matrix. Based on 3 factorial design, an optimized dispersion of lipid nanoparticles (solid lipid:surfactant-4.5:1.0 wt.%) was developed, predisposed for the incorporation of iridoid glycosides by emulsification-sonication method. The encapsulation efficiency of the active substances was determined at nearly 90% (aucubin) and 77% (catalpol). Regarding the stability study, room temperature was found to be the most suitable for maintaining the expected physicochemical parameter values (particle size < 100 nm; polydispersity index < 0.3; zeta potential > |± 30 mV|). Characterization of the lipid matrix confirmed the nanometer size range of the resulting carriers (below 100 nm), as well as the presence of the lipid in the stable β' form.
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http://dx.doi.org/10.3390/molecules26113161DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8198468PMC
May 2021

Sweet surfactants I: Fatty acid esters of sucralose.

Food Chem 2021 Oct 20;358:129827. Epub 2021 Apr 20.

Faculty of Chemistry, Adam Mickiewicz University, Poznań, Uniwersytetu Poznańskiego 8, 61-614 Poznań, Poland.

Surface active agents derived from the non-toxic sweetener sucralose and fatty acids of different chain length were synthesized. Obtained compounds were characterized chemically and with regard to their properties as emulsifying agents, antimicrobial preservatives and fat-soluble sweeteners. Results show that sucralose-fatty acid esters are possible multi-purpose additives, compatible with both cosmetic and edible emulsions, as well as purely oil-based, waterless formulations. Their relative effectiveness in those applications varies, and is highly dependent on the fatty acid chain length, with hydrophobic/hydrophilic character strongly impacting both emulsifying and antimicrobial properties. While the structural differences between sucrose and sucralose proved to be enough to push all of the newly synthesized compounds out of the detergent/solubilizer category of surfactants, the retention of the substrate's high sweetness is an indication that non-bitter compounds with washing capabilities are possible to obtain.
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http://dx.doi.org/10.1016/j.foodchem.2021.129827DOI Listing
October 2021

Acute aerobic exercise enhances cortical connectivity between structures involved in shaping mood and improves self-reported mood: An EEG effective-connectivity study in young male adults.

Int J Psychophysiol 2021 04 25;162:22-33. Epub 2021 Jan 25.

Psychophysiology Laboratory, Institute of Psychology, Jagiellonian University, Kraków, Poland.

There seems to be a general consensus among researchers that acute aerobic exercise (exercise hereafter) improves mood, but the neural mechanisms which drive these effects are far from being clear. The current study investigated the cortical connectivity patterns that underlie changes in mood after exercise. Twenty male adults underwent three different experimental protocols that were carefully controlled in terms of underlying metabolism and were administered in a randomized order: moderate-intensity continuous exercise, high-intensity interval exercise, and seated rest condition. Before and after each experimental protocol, we collected data on the participants' mood using the UMACL questionnaire and recorded their resting-state EEG. We focused on the effective connectivity patterns exerted by the dorso-lateral prefrontal cortex (dlPFC) over the temporal region (TMP), as these are important cortical structures involved in shaping mood. The cortical connectivity patterns in the resting-state EEG were evaluated using the directed transfer function (DTF), which is an autoregressive effective connectivity method. The results showed that both moderate-intensity exercise and high-intensity interval exercise improved participants' self-reported mood. Crucially, this improvement was accompanied by stronger influences of dlPFC over TMP. The observed changes in the effective connectivity patterns between dlPFC and TMP might help to better understand the effects of exercise on mood.
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http://dx.doi.org/10.1016/j.ijpsycho.2021.01.016DOI Listing
April 2021

Rhesus and cynomolgus macaque immunoglobulin heavy-chain genotyping yields comprehensive databases of germline VDJ alleles.

Immunity 2021 02 22;54(2):355-366.e4. Epub 2021 Jan 22.

Department of Microbiology, Tumor and Cell Biology, Karolinska Institutet, Stockholm 171 77, Sweden. Electronic address:

Definition of the specific germline immunoglobulin (Ig) alleles present in an individual is a critical first step to delineate the ontogeny and evolution of antigen-specific antibody responses. Rhesus and cynomolgus macaques are important animal models for pre-clinical studies, with four main sub-groups being used: Indian- and Chinese-origin rhesus macaques and Mauritian and Indonesian cynomolgus macaques. We applied the (Ig) gene inference tool IgDiscover and performed extensive Sanger sequencing-based genomic validation to define germline VDJ alleles in these 4 sub-groups, comprising 45 macaques in total. There was allelic overlap between Chinese- and Indian-origin rhesus macaques and also between the two macaque species, which is consistent with substantial admixture. The island-restricted Mauritian cynomolgus population displayed the lowest number of alleles of the sub-groups, yet maintained high individual allelic diversity. These comprehensive databases of germline IGH alleles for rhesus and cynomolgus macaques provide a resource toward the study of B cell responses in these important pre-clinical models.
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http://dx.doi.org/10.1016/j.immuni.2020.12.018DOI Listing
February 2021

Synergy of endoplasmic reticulum aminopeptidase 1 and 2 (ERAP1 and ERAP2) polymorphisms in atopic dermatitis: Effects on disease prevalence.

Hum Immunol 2021 Feb 9;82(2):121-123. Epub 2020 Dec 9.

Laboratory of Immunogenetics and Tissue Immunology, Hirszfeld Institute of Immunology and Experimental Therapy, Polish Academy of Sciences, Wrocław, Poland. Electronic address:

Endoplasmic reticulum aminopeptidases ERAP1 and ERAP2 trim peptides to a length of 8-10 amino acids optimal for binding by HLA class I molecules. Although these two enzymes may work separately, but they may also form a heterodimer of enhanced trimming efficiency. We have earlier described a role for ERAP1 single nucleotide polymorphism rs26618 and HLA-C*05:01 as risk factors for atopic dermatitis (AD). Here, we examined whether ERAP2 single nucleotide polymorphism rs2248374, determining the presence or absence of the functional form of enzyme, would influence the rs26618 effect. Out of nine rs2248374 - rs26618 genotypic combinations, only one, rs2248374*A/A - rs26618*C/C, was associated with a risk of AD. Interestingly, the odds ratio increased from 1.10 (CI95%: 0.72; 1.69; p = 0.657) for ERAP2 rs2248374*A/A and 1.88 (CI95%: 1.07; 3.28; p = 0.025) for ERAP1 rs26618*C/C to 3.36 (CI95%: 1.41; 8.01; p = 0.004) for their combination, therefore revealing a synergistic effect.
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http://dx.doi.org/10.1016/j.humimm.2020.11.004DOI Listing
February 2021

Synthesis, Characterization and Use of Mesoporous Silicas of the Following Types SBA-1, SBA-2, HMM-1 and HMM-2.

Materials (Basel) 2020 Oct 1;13(19). Epub 2020 Oct 1.

Faculty of Chemistry, Adam Mickiewicz University in Poznań, Uniwersytetu Poznańskiego 8, 61-614 Poznań, Poland.

Mesoporous silicas have enjoyed great interest among scientists practically from the moment of their discovery thanks to their unique attractive properties. Many types of mesoporous silicas have been described in literature, the most thoroughly MCM-41 and SBA-15 ones. The focus of this review are the methods of syntheses, characterization and use of mesoporous silicas from SBA (Santa Barbara Amorphous) and HMM (Hybrid Mesoporous Materials) groups. The first group is represented by (i) SBA-1 of three-dimensional cubic structure and Pm3n symmetry and (ii) SBA-2 of three-dimensional combined hexagonal and cubic structures and P6/mmc symmetry. The HMM group is represented by (i) HMM-1 of two-dimensional hexagonal structure and p6mm symmetry and (ii) HMM-2 of three-dimensional structure and P6/mmc symmetry. The paper provides comprehensive information on the above-mentioned silica materials available so far, also including the data for the silicas modified with metal ions or/and organic functional groups and examples of the materials applications.
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http://dx.doi.org/10.3390/ma13194385DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7579092PMC
October 2020

Noninvasive evaluation of the influence of aucubin-containing cosmetic macroemulsion on selected skin parameters.

J Cosmet Dermatol 2021 Mar 21;20(3):1022-1030. Epub 2020 Sep 21.

Department of Applied Chemistry, Faculty of Chemistry, Adam Mickiewicz University, Poznań, Poznan, Poland.

Background: Objective evaluation of skin condition on the basis of noninvasive methods has become obligatory and may be a good strategy for verifying the efficiency of new cosmetic active ingredients.

Aim: The aim of this study was to assess the influence of aucubin-containing cosmetic macroemulsion on skin parameters using the skin testing equipment.

Patients/methods: The study was carried out on the group of 25 female volunteers of the mean age 27 ± 2 years. The skin parameters were measured using the following devices: Tewameter TM 300 (transepidermal water loss, TEWL), Corneometer CM 825 (skin hydration), Cutometer MPA 580 (skin elasticity), Visioscan VC 98 (skin topography), and Visioline VL 650 (skin macrorelief). All measurements were performed on the inner part of the left forearm.

Results: The skin parameters measured revealed the beneficial influence of aucubin on TEWL and skin hydration level. The application of aucubin-enriched cream caused more significant improvements of all determined SELS parameters, in comparison with cream without it. An analogous tendency was noticed in the case of skin macrorelief parameters; the addiction of the active ingredient led to a decrease in the value of total wrinkle area and diminished the mean length and depth of single wrinkles.

Conclusions: Noninvasive methods of skin testing have provided a possibility of objective evaluation of the action of new active ingredients in cosmetic formulations. The study has proved the positive influence of aucubin on effectiveness of cosmetic macroemulsions, diminishing TEWL, increasing skin hydration level, and improving the values of SELS and skin macrorelief parameters.
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http://dx.doi.org/10.1111/jocd.13649DOI Listing
March 2021

Method development for determination of trace amounts of palladium in environmental water samples by ICP-MS/MS after pre-concentration on thiol-functionalized MCM-41 materials.

Talanta 2020 Sep 12;217:121004. Epub 2020 Apr 12.

Faculty of Chemistry, University of Bialystok, K. Ciołkowskiego 1K, 15-245, Białystok, Poland. Electronic address:

The anthropogenic cycle of Pd in the environment, its fate and impact is still unknown due to limitations of measurement techniques. For separation and pre-concentration of Pd(II) ions, mesoporous silica materials MCM-41 were synthesized and functionalized with different amounts of 3-mercaptopropyltrimethoxysilane (MPTMS) by co-condensation and grafting methods. The structural and textural properties of materials were characterized using XRD, TEM, SEM and BET techniques. The results proved that functionalization with thiol groups did not significantly affect structural and textural parameters of synthesized sorbents. The Pd(II) ions were quantitatively retained on sorbents functionalized by grafting in acidic solutions (pH 2), efficiently eluted with 0.1 mol L thiourea solution in 1 mol L HCl and determined by electrothermal atomic absorption spectrometry (ETAAS). The limit of detection (LOD) of the developed SPE ETAAS method was 0.06 ng mL, and the pre-concentration factor was 30. For analysis of Pd in environmental water samples inductively coupled plasma mass spectrometry (ICP-MS) in MS/MS mode was used. Spectral interferences on Pd caused by the presence of Sr in water samples were eliminated using helium (5 mL min) or ammonia (7 mL min) gas in collision/reaction cell. The developed SPE ICP-MS method is characterized by good selectivity in the presence of interfering elements and chloride ions and detection limit of 0.0002 ng mL. Its accuracy was confirmed by analysis of spiked water samples. The application of ICP-MS together with efficient separation/pre-concentration of analyte on thiol-functionalized MCM-41 sorbents allows to determine Pd in environmental water samples at pg mL level.
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http://dx.doi.org/10.1016/j.talanta.2020.121004DOI Listing
September 2020

KIR and HLA-C genes in male infertility.

J Assist Reprod Genet 2020 Aug 20;37(8):2007-2017. Epub 2020 May 20.

Department of Clinical Immunology, Laboratory of Immunogenetics and Tissue Immunology, Ludwik Hirszfeld Institute of Immunology and Experimental Therapy, Polish Academy of Sciences, Wrocław, Poland.

Purpose: Approximately 50% of men reporting to clinics for assisted reproduction have abnormal sperm parameters; we therefore considered whether they differ from fertile males in terms of the frequency of KIR and HLA-C genes, suggesting the involvement of NK cells and some T cells in the inflammatory reaction that can occur in the testes, vas deferens, or epididymis.

Method: We tested a total of 1064 men: 445 of them were patients who, together with their female partners, participated in in vitro fertilization (IVF), 298 men whose female partners suffered from recurrent spontaneous abortion. Three hundred twenty-one fertile men constituted the control group. KIRs were genotyped using KIR Ready Gene kits and HLA-C by PCR-SSP methods.

Results: We found differences in KIR gene frequencies between men who became fathers via natural conception and men who participated in in vitro fertilization for KIR2DL2 (p/p = 0.0015/0.035, OR = 1.61), KIR2DL5 gr.2 (p/p = 0.0023/0.05, OR = 1.64), KIR2DS2 (p/p = 0.0019/0.044, OR = 1.59), and KIR2DS3 (p/p = 0.0016/0.037, OR = 1.67). KIRs in Cen AA region were significantly overrepresented in fertile males than in IVF males (p/p = 0.0076/0.03, OR = 0.67), whereas Cen AB + Cen BB frequency was higher in IVF males than in fertile males (p/p = 0.0076/0.03, OR = 1.50). We also observed a limited association in KIR-HLA-C combinations.

Conclusion: Fertile men differ in profile of KIR genes and KIR-HLA-C combinations from men participating in IVF.
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http://dx.doi.org/10.1007/s10815-020-01814-6DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7467998PMC
August 2020

Factors Predicting Response to the Recovery-Oriented Cognitive Behavioural Workshop for Persons Diagnosed with Schizophrenia.

Community Ment Health J 2020 08 1;56(6):1115-1120. Epub 2020 Apr 1.

First Department of Psychiatry, Institute of Psychiatry and Neurology, Sobieskiego 9, 02-957, Warsaw, Poland.

A recovery-oriented, cognitive behavioural workshop for service users diagnosed with schizophrenia was developed, implemented and evaluated in a pilot study. Further analysis is required regarding factors which contribute to better treatment response, as this will provide useful information for workshop adaptation. Secondary multilevel model analyses were performed to determine whether workshop and booster session attendance, as well as sociodemographic variables such as gender, age, education, and duration of illness, predicted workshop responsiveness. Results showed that completers had lower responsiveness to the workshop in terms of confidence and hope, whereas those who attended an online booster session demonstrated better responsiveness as to psychosocial functioning. Longer duration of illness and older age generally predicted lower intervention responsiveness. In conclusion, adaptations utilising more booster sessions and accommodating older participants with longer duration of illness are required, as is further workshop evaluation in a randomised controlled study.
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http://dx.doi.org/10.1007/s10597-020-00595-7DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7289781PMC
August 2020

Loading, release profile and accelerated stability assessment of monoterpenes-loaded solid lipid nanoparticles (SLN).

Pharm Dev Technol 2020 Sep 23;25(7):832-844. Epub 2020 Mar 23.

Faculty of Pharmacy, University of Coimbra, Coimbra, Portugal.

Glycerol monostearate solid lipid nanoparticles (SLN) were produced by hot high-pressure homogenization technique to load alpha-pinene, citral, geraniol or limonene. SLN were composed of 1 wt.% monoterpene, 4 wt.% of Imwitor 900K as a solid lipid and 2.5 wt.% of Poloxamer188 as a surfactant. Empty SLN consisted of 5 wt.% of Imwitor 900K and 2.5 wt.% of Poloxamer188. The mean particles size (Z-Ave) and polydispersity index (PDI) of SLN were analyzed by dynamic light scattering (DLS), while the zeta potential (ZP) of each formulation were measured by electrophoretic light scattering. LUMiSizer was applied to calculate the velocity distribution in the centrifugal field and instability index. Drug release profile from SLN was analyzed using Franz cell diffusion cells assayed by UV-Vis spectrophotometry, whereas the gas chromatography technique was applied to determine the encapsulation parameters of volatile monoterpenes. The matrix state, polymorphism and phase behavior of SLN were studied by X-ray diffraction (XRD, low and wide angles) and differential scanning calorimetry (DSC). Selected monoterpenes were successfully loaded in glycerol monostearate SLN. A burst release profile within the first 15 min was observed for all formulations, being the modified release profile dependent on the type of monoterpene and on the encapsulation efficiency.
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http://dx.doi.org/10.1080/10837450.2020.1744008DOI Listing
September 2020

Is the TAP2 single nucleotide polymorphism rs241447 truly associated with psoriasis in Poles?

Hum Immunol 2020 Feb - Mar;81(2-3):85-90. Epub 2020 Jan 31.

Laboratory of Immunogenetics and Tissue Immunology, Ludwik Hirszfeld Institute of Immunology and Experimental Therapy, Polish Academy of Sciences, Wrocław, Poland. Electronic address:

Psoriasis vulgaris (PsV) is strongly associated with HLA-C*06:02. HLA class I molecules present antigenic peptides to CD8 T lymphocytes. Peptide transport from cytosol to the endoplasmic reticulum is mediated by a transporter associated with antigen processing (TAP) composed of TAP1 and TAP2 polymorphic proteins. Here, we compared the distribution of three coding single nucleotide polymorphisms (SNPs), rs1057141 in TAP1 and rs1800454 and rs241447 in TAP2 as well as the HLA-C*06:02 allele in 438 patients diagnosed with PsV and 493 control individuals. In patients and controls non-stratified by HLA-C*06:02, TAP2 rs241447 was associated with PsV but other TAP1 and TAP2 SNPs were not. By contrast, stratification according to the Svejgaard and Ryder formula, as well as a logistic regression approach and haplotype analysis demonstrated that the effect of TAP2 rs241447 was entirely related to the presence of HLA-C*06:02. The secondary effect of TAP2 rs241447 in relation to primary effect of HLA-C*06:02 resulted from linkage disequilibrium (albeit not strong) between both markers. We conclude that joint coexistence of HLA-C*06:02 and the TAP2 rs241447C risk allele on the extended haplotype might explain the effect of TAP2 observed by us.
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http://dx.doi.org/10.1016/j.humimm.2020.01.005DOI Listing
December 2020

Association of Soluble HLA-G Plasma Level and Genetic Polymorphism With Pregnancy Outcome of Patients Undergoing Fertilization Embryo Transfer.

Front Immunol 2019 14;10:2982. Epub 2020 Jan 14.

Laboratory of Immunogenetics and Tissue Immunology, Department of Clinical Immunology, Ludwik Hirszfeld Institute of Immunology and Experimental Therapy, Polish Academy of Sciences, Wrocław, Poland.

Infertility is currently a growing problem observed around the world and is estimated to affect between 8 and 12% of reproductive-aged couples worldwide. Artificial reproductive techniques are the last chance for couples seeking their own child. Human leukocyte antigen (HLA)-G expression has been suggested as an immunomodulatory molecule that influences pregnancy outcome. The gene encodes either membrane-bound or/and soluble proteins. The aim of this study was the evaluation of the role of soluble HLA-G (sHLA-G) and its gene polymorphism in successful implantation after fertilization embryo transfers (IVF-ETs) in different clinical protocols. We tested the polymorphism in three positions: rs1632947: c.-964G>A; rs1233334: c.-725G>C/T in promoter region; rs371194629: c.65_66insATTTGTTCATGCCT in 3' untranslated region of exon 8, in 389 patients who underwent IVF-ETs and 320 women with healthy children born after natural conception. Among the patient group, 239 women were with recurrent implantation failure and 117 women had an ongoing pregnancy or a child born after IVF-ET. We found that certain rs1632947-rs1233334-rs371194629 HLA-G haplotypes and diplotypes were associated with infertility, while others were protective. The lowest secretors of sHLA-G were G-C-ins haplotype carriers (37.21 IU/ml), while the highest -G-C-del carriers (73.80 IU/ml). Other haplotype carriers were intermediate secretors. In our study, regardless of possessed haplotype by the patient, 59.73 IU/ml sHLA-G was the threshold value with the best sensitivity (58.82%) and specificity (66.10%) to discriminate patients who achieved and maintained pregnancy from those who did not conceive or they had miscarriage ( = 0.0085; likelihood ratio, 1.74; 95% CI = 0.55-0.78). However, we do not exclude that factors other than sHLA-G may also contribute to complications in pregnancy. In addition, we found that IVF patients in cycles when frozen/thawed embryo was transferred secreted higher soluble HLA-G levels than patients with fresh embryo transferred ( = 0.021). Moreover, correlation analysis of sHLA-G concentration measured before and after embryo transfer for particular patients indicated short ovarian stimulation with gonadotropin-releasing hormone antagonist as more beneficial than long protocol with gonadotropin-releasing hormone agonist. Our study confirms a role of polymorphism in infertility and soluble HLA-G in the early stages of pregnancy.
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http://dx.doi.org/10.3389/fimmu.2019.02982DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6971053PMC
December 2020

Development and Optimization of Alpha-Pinene-Loaded Solid Lipid Nanoparticles (SLN) Using Experimental Factorial Design and Dispersion Analysis.

Molecules 2019 Jul 24;24(15). Epub 2019 Jul 24.

Department of Pharmaceutical Technology, Faculty of Pharmacy, University of Coimbra (FFUC), Pólo das Ciências da Saúde, Azinhaga de Santa Comba, 3000-548 Coimbra, Portugal.

The encapsulation of bicyclic monoterpene α-pinene into solid lipid nanoparticles (SLN) is reported using experimental factorial design, followed by high-end dispersion analyzer LUMiSizer. This equipment allows the characterization of the α-pinene-loaded SLN instability phenomena (e.g., sedimentation, flotation or coagulation), as well as the determination of the velocity distribution in the centrifugal field and the particle size distribution. In this work, SLN were produced by hot high-pressure homogenization technique. The influence of the independent variables, surfactant and lipid ratio on the physicochemical properties of SLN, such as mean particle size (Z-Ave), polydispersity index (PDI) and zeta potential (ZP), was estimated using a 2-factorial design. The Z-Ave and PDI were analyzed by dynamic light scattering, while ZP measurements were recorded by electrophoretic light scattering. Based on the obtained results, the optimal SLN dispersion was composed of 1 wt.% of α-pinene, 4 wt.% of solid lipid (Imwitor 900 K) and 2.5 wt.% of surfactant (Poloxamer 188), depicting 136.7 nm of Z-Ave, 0.170 of PDI and 0 mV of ZP. Furthermore, LUMISizer has been successfully used in the stability analysis of α-pinene-loaded SLN.
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http://dx.doi.org/10.3390/molecules24152683DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6696006PMC
July 2019

Towards a gamete matching platform: using immunogenetics and artificial intelligence to predict recurrent miscarriage.

NPJ Digit Med 2019 7;2:12. Epub 2019 Mar 7.

2Department of Clinical Immunology, Laboratory of Immunogenetics and Tissue Immunology, Ludwik Hirszfeld Institute of Immunology and Experimental Therapy, Polish Academy of Sciences, ul. Rudolfa Weigla 12, 53-114, Wrocław, Poland.

The degree of Allele sharing of the Human Leukocyte Antigen (HLA) genes has been linked with recurrent miscarriage (RM). However, no clear genetic markers of RM have yet been identified, possibly because of the complexity of interactions between paternal and maternal genes during embryo development. We propose a methodology to analyse HLA haplotypes from couples either with histories of successful pregnancies or RM. This article describes a method of RM genetic-risk calculation. The proposed HLA representation techniques allowed us to create an algorithm (IMMATCH) to retrospectively predict RM with an AUC = 0.71 ( = 0.0035) thanks to high-resolution typing and the use of linear algebra on peptide binding affinity data. The algorithm features an adjustable threshold to increase either sensitivity or specificity, allowing a sensitivity of 86%. Combining immunogenetics with artificial intelligence could create personalised tools to better understand the genetic causes of unexplained infertility and a gamete matching platform that could increase pregnancy success rates.
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http://dx.doi.org/10.1038/s41746-019-0089-xDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6550222PMC
March 2019

ERAP, KIR and HLA-C gene interaction in susceptibility to recurrent spontaneous abortion in the Polish population.

Hum Immunol 2019 May 21;80(5):344-348. Epub 2019 Feb 21.

Laboratory of Immunogenetics and Tissue Immunology, Department of Clinical Immunology, Ludwik Hirszfeld Institute of Immunology and Experimental Therapy, Polish Academy of Sciences, ul. Rudolfa Weigla 12, 53-114 Wrocław, Poland. Electronic address:

Endoplasmic reticulum aminopeptidases ERAP1 and ERAP2 trim peptides to generate stable antigenic epitopes for their presentation by HLA class I (HLA-I) molecules to T cell receptor. By influencing the peptide repertoire of HLA-I molecules, they affect also the interactions of HLA-I with killer cell immunoglobulin-like receptors (KIRs) of natural killer (NK) cells. HLA-C is the only polymorphic HLA-I molecule present on the trophoblast. In this study we investigated the role of ERAP1 and ERAP2 polymorphisms in the context of KIR and HLA-C genes in women suffering from recurrent spontaneous abortion (RSA) in the Polish population. We used TaqMan genotyping assays for ERAP1 rs27044, rs30187, rs2287987, rs26618, rs2665 and ERAP2 rs2248374; PCR-SSP methods for KIR and HLA-C genotyping. We tested 285 women who experienced recurrent spontaneous abortion (RSA) and 319 fertile women. We observed a significant association of ERAP1 rs30187TT genotype with RSA (p = 0.02, OR = 1.89, 95%CI = 1.11-3.21), however the most striking association was found in comparison of patients and controls with ERAP1 rs30187TT and KIR Bx genotypes (p = 0.006, p = 0.036, OR = 2.40, 95%CI = 1.27-4.52). Moreover, this effect was even stronger in HLA-C2 positive patients (p = 0.0031, p = 0.019, OR = 3.46, 95%CI = 1.48-8.11). Other weaker associations of the remaining tested ERAP single nucleotide polymorphisms with RSA were also presented. In conclusion, ERAP1 rs30187TT genotype itself increased susceptibility to RSA but this effect was much stronger in patients positive for HLA-C2 and KIR Bx genotypes.
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http://dx.doi.org/10.1016/j.humimm.2019.02.010DOI Listing
May 2019

ERAP1-ERAP2 haplotypes are associated with ankylosing spondylitis in Polish patients.

Hum Immunol 2019 May 19;80(5):339-343. Epub 2019 Feb 19.

Laboratory of Immunogenetics and Tissue Immunology, Ludwik Hirszfeld Institute of Immunology and Experimental Therapy, Polish Academy of Sciences, Wrocław, Poland. Electronic address:

The objective of this case-control study was to evaluate the role of four single-nucleotide polymorphisms in the ERAP1 (rs2287987, rs30187, rs27044) and ERAP2 (rs2248374) genes and their haplotypes in predicting the risk for ankylosing spondylitis (AS) on a well-defined Polish population. Our study confirmed the strong association between the HLA-B*27 allele and the disease. For all tested ERAP1 SNPs we found significant differences in the minor allele and genotype distribution between patients and controls. The strongest association with AS was observed for rs30187. The minor T allele and homozygous TT genotype of this SNP significantly increased disease risk (OR = 1.56, 95%CI = 1.22-1.99, p = 0.0004 and OR = 2.52, 95%CI = 1.50-4.25, p = 0.001, respectively). In the case of rs2287987, minor C allele exerted a protective effect (OR = 0.64, 95%CI = 0.46-0.88, p = 0.008). In contrast to ERAP1, we observed no effect of rs2248374 in ERAP2 on the disease. We also carried out ERAP1-ERAP2 haplotype analysis to demonstrate a possible association of both genes with AS. Results showed that the haplotype H4, containing ERAP1 SNPs associated with high enzymatic activity, together with the presence of ERAP2 expression, significantly increased the risk of AS (OR = 1.97, 95% CI = 1.21-3.21, p = 0.048). By contrast, the haplotype H5 coding for low activity of ERAP1 and the lack of ERAP2 expression was strongly protective (OR = 0.41, 95% CI = 0.23-0.72, p = 0.008).
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http://dx.doi.org/10.1016/j.humimm.2019.02.004DOI Listing
May 2019

Modifying release of poorly soluble active pharmaceutical ingredients with the amine functionalized SBA-16 type mesoporous materials.

J Biomater Appl 2019 04 21;33(9):1214-1231. Epub 2019 Feb 21.

1 Department of Pharmaceutical Technology, Poznan University of Medical Sciences, Poznań, Poland.

SBA-16 and two modified SBA-16 type ordered mesoporous silica were used as the carriers for ibuprofen (anti-inflammatory drug) and furosemide (loop diuretic drug). Modification of the solid carrier was prepared with chitosan or N-3[(amino(poly-propylenoxy)]aminopropyltrimethoxysilane. The samples of carriers and carrier-drug loaded materials were characterized by X-ray diffraction, N adsorption, Fourier-transform infrared spectroscopy, thermogravimetry, and differential scanning calorimetry. The release profiles of active pharmaceutical ingredients were performed in media with different pH in the USP 2 apparatus as well as in two biorelevant media (fasted state simulated gastric fluid and fasted state small intestinal fluid) in USP 4 apparatus. The loading of active substances into mesoporous materials was performed with modified immersion method. The maximum content of deposited drug in mesoporous material was close to 12.0 and 2.2 wt.% for ibuprofen and furosemide, respectively. After drug adsorption, the reduction of BET surface area, pore volume and pore diameter of non-modified and modified SBA-16 was observed, while the cubic arrays of siliceous matrix were well preserved. The release profiles of ibuprofen and furosemide loaded in mesoporous materials in media with different pH and biorelevant fasted state simulated gastric fluid and fasted state small intestinal fluid showed that the new SBA-16 type materials modify the release profiles of furosemide, increasing the dissolution rate of these substances in the medium at pH 1.2. The cytotoxicity of the materials and permeability of drugs after their loading on SBA-16 materials were evaluated on Caco-2 model. The results of our study showed that mesoporous materials did not exert cytotoxic effects and did not influence on the permeability of both active pharmaceutical ingredients in relation to pure substances.
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http://dx.doi.org/10.1177/0885328219830823DOI Listing
April 2019

Duffy blood group system - the frequency of Duffy antigen polymorphisms and novel mutations in the Polish population.

Transfus Apher Sci 2019 Apr 6;58(2):156-161. Epub 2019 Jan 6.

Hirszfeld Institute of Immunology and Experimental Therapy, Polish Academy of Sciences, Wrocław, Poland; Faculty of Physical Education and Physiotherapy, Opole University of Technology, Prószkowska 76, 45-758 Opole, Poland. Electronic address:

Duffy blood group genes are highly polymorphic with the distribution of alleles varying between different populations and ethnic groups. The aim of this study was to genotype Duffy blood group antigens and to establish FY alleles frequency in the Polish population and screen for novel FY gene mutations. Duffy phenotype and genotype frequencies analysis was based on studies of 596 persons. All these subjects were genotyped by high-resolution melting (HRM) method. It was shown that phenotype Fy(a+b+), defined by genotypes FY*A/FY*B (33%), FY*A/FY*B298A (13%), and FY*A/FY*02W.01 (2.8%) was the most common in Polish population (˜49%), followed by Fy(a-b+), ˜29%, determined by genotypes arising from FY*B allele and all its variants. Fy(a+b-) phenotype occurred with a frequency of 21.3% and was defined by the following genotypes: FY*A/A (21%), and FY*A/02N.01 (0.3%). Among the Polish population the frequencies of FY*A, FY*B, and FY*B298A alleles were 45.7%, 36% and 15.5%, respectively. The alleles FY*B298A and FY*B combined together, represented higher frequency (51%) than FY*A. Alleles FY*02W.01 and FY*02N.01 had frequencies 2.51% and 0.25%, respectively. The distribution of Duffy genotypes in the Polish population was in accordance with Hardy-Weinberg equilibrium (p = 0.9682). Alleles in the genotypes are independent from each other (r = 0.0278, R = 0.00077). New mutations identified in the promoter region (c.-79T > C) and the coding region of the FY gene (c.147C > A and c.175 G > A) did not affect the Duffy antigen expression on erythrocyte. Although FY alleles frequency is known in different populations, no data for Polish population is available.
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http://dx.doi.org/10.1016/j.transci.2018.12.020DOI Listing
April 2019

Hope and its dimensions in relation to clinical recovery: A cross-sectional study among people with psychotic disorders.

Psychiatr Rehabil J 2019 Jun 17;42(2):139-146. Epub 2018 Dec 17.

First Department of Psychiatry, Institute of Psychiatry and Neurology.

Objective: Hope is a key component of personal recovery. There is limited evidence regarding the association of hope with the level of functioning in individuals with psychosis. It is also not clear which dimensions of hope are most strongly related to clinical recovery. Thus, this study aims to explore the relationships of hope and its dimensions with various indicators of clinical recovery such as overall psychopathology, depression and global functioning among people with psychotic disorders.

Method: The Integrative Hope Scale (IHS; Schrank, Woppmann, Sibitz, & Lauber, 2011) was administered to 110 people with psychotic disorders. Multiple regression analysis was used to investigate the associations of the IHS total score and its four subscales (i.e., Trust and Confidence, Lack of Perspective, Positive Future Orientation, and Social Relations and Personal Value) with overall psychiatric symptoms, depression, and general functioning.

Results: A total level of Hope was not associated with overall psychopathology or global functioning; however, it showed a significant negative relationship with severity of depression. A stronger feeling of a Lack of Perspective turned out to be associated with more severe depression and a greater intensity of psychopathological symptoms. The relationships of the remaining dimensions of Hope with the indicators of clinical recovery were found to be nonsignificant.

Conclusion And Implications For Practice: The findings suggest that combining Hope-enhancement strategies with interventions targeting symptoms may increase the effectiveness of rehabilitation programs for people with psychosis. They also point to the sense of a Lack of Perspective as the aspect of Hope most strongly related to clinical recovery. (PsycINFO Database Record (c) 2019 APA, all rights reserved).
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http://dx.doi.org/10.1037/prj0000340DOI Listing
June 2019
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