Publications by authors named "Iraj Salimikia"

9 Publications

  • Page 1 of 1

Chemical Composition, Apoptotic Activity, and Antiparasitic Effects of Essential Oil against Protoscoleces.

Molecules 2021 Feb 8;26(4). Epub 2021 Feb 8.

Razi Herbal Medicines Research Center, Lorestan University of Medical Sciences, Khorramabad 6813833946, Iran.

Background: Today, the present protoscolicidals used to minimize the serious risks during hydatid cyst surgery are not completely safe and have various adverse side effects. The present study aimed to evaluate the chemical composition and apoptotic activity of essential oil (FMEO) as well as its in vitro and ex vivo protoscolicidal effects against hydatid cyst protoscoleces.

Methods: Gas chromatography/mass spectrometry (GC/MS) analysis was performed to determine the chemical composition of FMEO. Protoscoleces of hydatid cysts were collected from liver fertile hydatid cysts of infected sheep and were then treated with various concentrations of the essential oil (75, 150, and 300 µL/mL) for 5-60 min in vitro and ex vivo. Then, by using the eosin exclusion test, the viability of the protoscoleces was studied. The caspase-3-like activity of the FMEO-treated protoscoleces was also evaluated through the colorimetric protease assay Sigma Kit based on the manufacturer's instructions.

Results: According to GC/MS, the main constituents of the essential oil were terpinolene (77.72%), n-nonanal (4.47%), and linalool (4.35%), respectively. In vitro, the maximum protoscolicidal activity of FMEO was observed at the concentrations of 150 and 300 µL/mL, such that 100% of the protoscoleces were killed after 30 and 20 min of exposure, respectively. Based on the obtained findings, the results demonstrate that FMEO required a longer time to kill protoscoleces ex vivo; after 12 min of exposure to FMEO, only 13.4% of the protoscoleces remained alive. After 48 h of the treatment of protoscoleces, FMEO, in a dose-dependent manner and at doses of 75, 150, and 300 µL/mL, induced the activation of the caspase enzyme by 24.3, 35.3, and 48.3%, respectively.

Conclusions: Our findings demonstrate the potent protoscolicidal effects of FMEO in vitro and ex vivo; however, further studies are required to assess the safety and the efficiency of FMEO as a promising scolicidal agent in a preclinical model and clinical setting.
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February 2021

Iranian Herbal Medicines Against Hydatid Cyst Protoscoleces: A Systematic Review.

Infect Disord Drug Targets 2021 ;21(5):e270421188119

Department of Surgery, Lorestan University of Medical Sciences, Khorramabad, Iran.

Background: In recent years, more attention has been focused in the practice of both crude extract of medicinal plants, and the screening plant-derived compounds as substitute scolicidal agents during hydatid cyst surgery. The present study was designed to review the protoscolicidal effects of some Iranian herbal medicines against hydatid cyst protoscoleces.

Methods: English databases, including PubMed, Google Scholar, Web of Science, EBSCO, Science Direct, and Scopus were searched for publications worldwide related to protoscolicidal effects of Iranian herbal medicines without date limitation so that identify all published articles (in vitro, in vivo, clinical and case-control) have studied. Keywords included "Protoscolicidal", "Scolicidal", "Herbal medicines", "Extract", "Essential oil", "Plant", "In vitro", and "Iran". Moreover, the language of data collection was limited to English.

Results: In total, 40 papers up to 2020 were included in the present systematic review. The most studies were conducted on protoscolicidal activity of methanolic extracts (17 studies) followed by essential oils (15 studies), and aqueous extract (3 studies). The most commonly used part of herbs were leaves (21 herbs), seeds (8 herbs), and fruit (6 herbs), respectively. Moreover, the most prevalent herbal family was Lamiaceae (6 studies), followed by Apiaceae (5 studies) and Liliaceae (5 studies).

Conclusion: Considering the high efficacy of Iranian herbal medicines against hydatid cyst protoscoleces, it can be concluded that Iranian herbal medicines have ability to consider as new alternative protoscolicidal agents during hydatid cyst surgery; however, more clinical studies are required to discover the precise protoscolicida activity of Iranian medicines in animal and human subjects.
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October 2021

Effect of chitosan on infection: A systematic review.

Parasite Epidemiol Control 2020 Nov 21;11:e00189. Epub 2020 Oct 21.

Hepatitis Research Center, Lorestan University of Medical Sciences, Khorramabad, Iran.

Background: The preferred treatment for management of toxoplasmosis is the combined use of pyrimethamine and sulfadiazine. However, there are a wide number of adverse side effects with these medications. Recent research has focused on the use of chitosan for the treatment of infections. This review was performed to obtain a better understanding of the and effects of chitosan on strains.

Methods: The current study was carried out according to the PRISMA guideline and registered in the CAMARADES-NC3Rs Preclinical Systematic Review and Meta-analysis Facility (SyRF) database. The search was performed in five scientific databases, including Scopus, PubMed, Web of Science, EMBASE, and Google Scholar, with date limits of 1992 to December 2019. The search was restricted to articles published in the English language. The words and terms searched were "", "Chitosan", "nanoparticles" and "anti-toxoplasmosis" with AND or OR.

Results: Of 2500 manuscripts, 9 met the eligibility criteria for review. All studies used the RH strain of , with Me49 and PRU each included in one study. Five studies (56%) were performed , one study and 3 studies included and tests.

Conclusion: Considering the low toxicity and the high inhibitory potency of chitosan against , chitosan nanoparticles show potential as an alternative treatment for .
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November 2020

Application of aeration-assisted homogeneous liquid-liquid microextraction procedure using Box-Behnken design for determination of curcumin by HPLC.

J Sep Sci 2020 Jul 4;43(13):2513-2520. Epub 2020 May 4.

Razi Herbal Medicines Research Center, Lorestan University of Medical Sciences, Khorramabad, Iran.

A simple, efficient, and rapid sample preparation method based on aeration-assisted homogeneous liquid-liquid microextraction was developed for determination of curcumin in food samples by high-performance liquid chromatography. The centrifuge step has been eliminated in this procedure. The effects of some variables, such as pH, volume of extraction solvent, extraction time, and salt effect, were studied through a Box-Behnken design method. Under the optimum conditions, calibration curves of curcumin were linear in the range of 0.08-4000 μg/mL with R  = 0.997. Limit of detection and relative standard deviation were 0.019 μg/mL and 3.01%, respectively. The preconcentration factor achieved was 166. The proposed method was successfully applied to determination of curcumin in various food samples.
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July 2020

Campylobacter: A Review of New Promising Remedies with Medicinal Plants and Natural Antioxidants.

Mini Rev Med Chem 2020 ;20(15):1462-1474

Department of Infectious Diseases, Lorestan University of Medical Sciences, Khorramabad, Iran.

Campylobacter (curved bacteria) is considered one of the most important and common zoonotic bacteria and the three leading bacterial causes of gastroenteritis and diarrhea. Antibacterial resistance is growing and expanding. The aim of this review article is to report anti-Campylobacter medicinal plants. For this purpose, the search terms consisting of Campylobacter, medicinal plants, essential oil, extract, and traditional medicine were used to retrieve the relevant articles published in the journals indexed in Information Sciences Institute, Web of Science, PubMed, Scopus, Google Scholar, and Scientific Information Databases. Then, the findings of eligible articles were analyzed. According to the analysis, 71 medicinal plants were found to exert anti-Campylobacter effect. The active compounds of these plants are possibly nature-based antibiotic agents that are effective on Campylobacter. If these compounds are isolated, purified, and studied in pharmaceutical investigations, they can be used to produce nature-based, anti-Campylobacter antibiotics.
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May 2021

Biological applications of phytosynthesized gold nanoparticles using leaf extract of Dracocephalum kotschyi.

J Biomed Mater Res A 2019 03 6;107(3):621-630. Epub 2018 Dec 6.

Department of Pharmacognosy, Faculty of Pharmacy, Lorestan University of Medical Sciences, Khorramabad, Iran.

In this work, biosynthesis potentials of Dracocephalum kotschyi leaf extract for the production of gold nanoparticle (AuNPs) were studied, and the biological (catalytic, antibacterial, antioxidant, and anticancer) activities of studied AuNPs were evaluated. Different analytical techniques including UV-visible spectroscopy, X-ray diffraction (XRD), Fourier transform infrared spectroscopy (FTIR), scanning electron microscope (SEM), energy dispersive X-ray analysis, and transmission electron microscopy (TEM) were used for the characterization of AuNPs. Moreover, Different testing methods were used for evaluating biological activities of biosynthesized AuNPs. The formation of AuNPs was confirmed by color change and UV-visible spectroscopic analysis. Field emission (FE)-SEM and TEM images were used to characterize phytosynthesized AuNPs which were predominantly spherical in shape with size in the range of 5-21 nm. These spherical NPs were found to be 39.79 ± 5 nm in size as determined by dynamic light scattering particle size analyzer. XRD pattern confirms the crystalline nature of the biosynthesized nanoparticles. The phytoconstituents involved in the reduction and stabilization of nanoparticles have been identified using FTIR spectra. The phytosynthesized AuNPs showed effective antioxidant, antibacterial and catalytic reduction activities. Furthermore, they have inhibited H1229 and MCF-7 cancer cell lines proliferation in a dose-dependent manner. These results have supported that D. kotschyi leaf extract was very efficient for the synthesis of AuNPs, and synthesized NPs showed enhanced biological activities which make them suitable for biomedical applications. © 2018 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 107A: 621-630, 2019.
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March 2019

Phytochemical Analysis and Antioxidant Activity of Aerial Extracts.

Iran Red Crescent Med J 2016 Aug 21;18(8):e24836. Epub 2016 Jun 21.

Department of Pharmacognosy, Faculty of Pharmacy, Lorestan University of Medical Sciences, Khorramabad, IR Iran.

Background: Salvia, known as Maryam Goli in the Persian language, is an important genus that includes approximately 900 species in the Lamiaceae family. There are 58 Salvia species growing naturally in Iran, including Salvia . f. and Allen., which grows wild in the northeastern and central parts of the country.

Objectives: This study was designed to determine the chemical composition, in vitro antioxidant activity, and total phenol content of various extracts of .

Materials And Methods: Dried aerial parts of the plant were crushed, then sequentially extracted with n-hexane, ethyl acetate, and methanol. The fractions of were subjected to silica gel column chromatography and Sephedex LH-20. The antioxidant activities of these extracts were measured by ferric reducing antioxidant power (FRAP), and the total phenolic contents of the extracts were evaluated using Folin-Ciocalteu reagent.

Results: The separation and purification processes were carried out using different chromatographic methods. Structural elucidation was on the basis 1H-NMR and 13C-NMR spectral data, in comparison with that reported in the literature. The isolated compounds were salvigenin (1), luteolin (2), cirsiliol (3), β-sitosterol (4), and daucosterol (5). Ethyl acetate extract displayed the highest level of total antioxidants and total polyphenols compared to the other analyzed extracts (n-hexane and methanol). In the FRAP assay, ethyl acetate extract had the highest (230.4±10.5) FRAP value, followed by methanol (211.4 ± 8.3) and n-hexane (143.4 ± 12.04). Total phenol contents were calculated to be 13.8 ± 0.3, 58.25 ± 0.05, and 43.48 ± 0.38 mg of gallic acid/100 g in the n-hexane, ethyl acetate, and methanol extracts, respectively.

Conclusions: The above-mentioned compounds were isolated for the first time from . The antioxidant activity of this plant could be in part related to isolated flavonoids and sterols. The results of this study indicated that could be an important dietary source of phenolic compounds with high antioxidant capacity.
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August 2016

A new rearranged tricyclic abietane diterpenoid from Salvia chloroleuca Rech. f. & Allen.

Nat Prod Res 2016 15;30(1):120-4. Epub 2015 May 15.

c Faculty of Pharmacy, Medicinal Plants Research Center, Tehran University of Medical Sciences , Tehran , Iran.

Phytochemical investigation of the roots of Salvia chloroleuca led to the isolation and identification of a new rearranged abietane diterpenoid (1). Its structure was elucidated by interpretation of the 1D and 2D NMR spectra and completed by the analysis of the HR-ESI-MS data. Compound 1 is the secondly reported compound on a rearranged tricyclic abietane (4,5-seco-5,10-friedo-abietane) diterpenoid with a ketal functionality between C-2 and C-11. A plausible biosynthetic pathway of 1 was also proposed.
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July 2016

In vitro antioxidant and free radical scavenging activity of four Alkanna species growing in Iran.

Pharmacognosy Res 2015 Jan-Mar;7(1):100-4

Department of Pharmacognosy, Faculty of Pharmacy, Tehran University of Medical Sciences, Tehran, Iran.

Background: Medicinal plants are recognized as sources of natural antioxidants that can protect biological system from oxidative stress.

Objective: In this study, the antioxidant activities of four species of the Alkanna genus (Alkanna bracteosa, Alkanna frigida, Alkanna orientalis and Alkanna tricophila) were evaluated.

Materials And Methods: The dried roots of plant samples (100 g) were extracted separately by percolation method with MeOH/H2O (80/20) and four fractions were provided respectively with hexane, chloroform, ethyl acetate, butanol and water. Antioxidant activities were investigated by using 1,1-diphenyl-2-picrylhydrazyl, ferric thiocyanate and thiobarbituric acid methods and compared with quercetin (as positive control).

Results: The results indicated that the butanol fractions of four species had the highest antioxidant activity and radical scavenging activity compared with the other fractions studied (P < 0.05). The 50% effective concentration (half - effective doses) values of butanol fractions are less than quercetin and other fractions, so, these fractions showed potent antioxidant activity. This indicated that the active compounds in the root parts of Alkanna species dissolved in the butanol. All the fractions of four species of Alkanna had antioxidant activity, while, there were no significant differences (P > 0.05) between the total antioxidant activities of same fractions. The total antioxidant activity values of Alkanna fractions in a descending order are as follows: Butanol fraction > ethyl acetate fraction > total extract > hexane fraction > chloroform fraction > aqueous fraction.

Conclusion: The antioxidant activities of butanol fractions of samples were higher than quercetin. This may be because most of the active compounds of Alkanna species dissolved in the butanol.
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January 2015