Publications by authors named "Ines Aouf"

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Cytotoxicity and Antiviral Activities of Haplophyllum tuberculatum Essential Oils, Pure Compounds, and Their Combinations against Coxsackievirus B3 and B4.

Planta Med 2021 Aug 22;87(10-11):827-835. Epub 2021 Jul 22.

Department of Analytical Chemistry, Applied Chemometrics and Molecular Modelling (FABI), Center for Pharmaceutical Research (CePhaR), Vrije Universiteit Brussel (VUB), Belgium.

is a plant commonly used in folk medicine to treat several diseases including vomiting, nausea, infections, rheumatism, and gastric pains. In the current study, essential oils, hydrosols, the pure compounds -(+)-limonene, -(-)-limonene, and 1-octanol, as well as their combinations -(+)-limonene/1-octanol and -(-)-limonene/1-octanol, were screened for their cytotoxicity on HEp-2 cells after 24, 48, and 72 h, and then tested for their activity against Coxsackievirus B3 and B4 (CV-B3 and CV-B4) at 3 different moments: addition of the plant compounds before, after, or together with virus inoculation. Results showed that the samples were more cytotoxic after 72 h than after 24 h or 48 h cell contact. However, the combinations -(+)-limonene/1-octanol and -(-)-limonene/1-octanol showed less effect on HEp-2 cells than pure -(+)-limonene and -(-)-limonene after 24 h, 48 h, and 72 h. 1-octanol exhibited the highest concentration causing 50% cytotoxicity (CC) on HEp-2 cells after 24 h (CC = 93 µg/mL) and 48 h (CC = 83 µg/mL). The antiviral assays showed that the tested samples exhibited potent inhibition of CV-B. IC values ranged from 0.66 µg/mL to 28.4 µg/mL. In addition, CV-B3 was more sensitive than CV-B4. Both CV-B strains are more inhibited when cells were pretreated with the plant compounds. The hydrosols have no effect, neither on HEp-2 cells nor on the virus. 1-octanol, -(-), and -(+)-limonene/1-octanol had important selectivity indexes over time. Although essential oils had potent antiviral activity, they can be considered for application in the pretreatment of cells. However, 1-octanol and the combinations are within the safety limits, and thus, they can be used as an active natural antiviral agent for CV-B3 and CV-B4 inhibition.
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http://dx.doi.org/10.1055/a-1538-5289DOI Listing
August 2021
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