Publications by authors named "Indu B"

15 Publications

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Comparative analysis of the immunomodulatory potential of caprine fetal adnexa derived mesenchymal stem cells.

Mol Biol Rep 2021 May 28;48(5):3913-3923. Epub 2021 May 28.

Division of Physiology and Climatology, ICAR-Indian Veterinary Research Institute, Izatnagar-243 122, Bareilly, U.P, India.

The caprine mesenchymal stem cells (MSCs) derived from fetal adnexa are highly proliferative. These cells possess tri-lineage differentiation potential and express MSC surface antigens and pluripotency markers with a wound-healing potential. This present study was conducted to compare the immunomodulatory potential of caprine MSCs derived from the fetal adnexa. Mid-gestation caprine uteri (2-3 months) were collected from the abattoir to isolate MSCs from amniotic fluid (cAF), amniotic sac (cAS), Wharton's jelly (cWJ) and cord blood (cCB), which were expanded and characterized at the 3 passage. These MSCs were then stimulated with inflammatory cytokines (IFN-γ and TNF-α) to assess the percentage of inhibition produced on peripheral blood mononuclear cells (PBMCs) proliferation. The percentage of inhibition on activated PBMCs proliferation produced by cWJ MSCs and cAS MSCs was significantly higher than cCB and cAF MSCs. The relative mRNA expression profile and immunofluorescent localization of different immunomodulatory cytokines and growth factors were conducted upon stimulation. The mRNA expression profile of a set of different cytokines and growth factors in each caprine fetal adnexa MSCs were modulated. Indoleamine 2, 3 dioxygenase appeared to be the major immunomodulator in cWJ, cAF, and cCB MSCs whereas inducible nitric oxide synthase in cAS MSCs. This study suggests that caprine MSCs derived from fetal adnexa display variable immunomodulatory potential, which appears to be modulated by different molecules among sources.
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http://dx.doi.org/10.1007/s11033-021-06383-0DOI Listing
May 2021

Expression profile of adhesion molecules in blastocyst vis-a-vis uterine epithelial cells.

Theriogenology 2021 Aug 5;170:36-45. Epub 2021 May 5.

Division of Physiology and Climatology, ICAR-Indian Veterinary Research Institute, Izatnagar, 243122, India.

Models using in vitro produced buffalo embryos and in vitro cultured uterine epithelial cells (UECs) may be useful in understanding the intricacies of embryo-uterine cross talk. In the present study, buffalo UECs were obtained from slaughterhouse derived non-gravid uterus. UECs monolayer was treated with steroids (10pg/ml estradiol for 24h and 3.14 ng/ml progesterone for another 5 days). In vitro produced buffalo blastocysts were co-cultured over steroid treated UECs monolayer and at 72 h of co-culture, embryo attachment rate was higher in UECs treated with steroids (71.86% vs. 26.55%) while no attachment was observed on plastic surface. Naturally hatched or assisted hatched blastocysts were co-cultured over UECs monolayer treated with 3.14ng/ml progesterone (P4), or without any treatment for 72 h and the effect of co-culture on the expression profile of adhesion related biomolecules was analyed in UECs and blastocysts. Cultured UECs and blastocysts cultured in embryo culture media were considered as control. It was observed that the expression of MUC1 in UECs was significantly (p < 0.05) higher in control group than treatment groups. The relative mRNA abundance of integrins and osteopontin was significantly (p < 0.05) higher in UECs and blastocysts of treatment groups than control group. Expression of IFN-τ was significantly higher (p < 0.05) in embryos co-cultured with UECs than other treatment groups. It can be concluded that P4 supplementation is required for the modulation of adhesion molecules and co-culture of blastocysts and UECs together affect the expression of adhesion molecules both in blastocyts and in UECs.
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http://dx.doi.org/10.1016/j.theriogenology.2021.04.018DOI Listing
August 2021

Uncovering the hidden bacterial ghost communities of yeast and experimental evidences demonstrates yeast as thriving hub for bacteria.

Sci Rep 2021 04 30;11(1):9394. Epub 2021 Apr 30.

Department of Plant Sciences, School of Life Sciences, University of Hyderabad, Hyderabad, India.

Our major concern was to address "yeast endobacteria" which was based on a few reports in the recent past where bacteria may find yeast as a niche for survival. In this study, we report the microbiota of twenty-nine axenic yeast cultures recovered from different habitats based on their 16S rRNA gene-amplicon metagenomes. Yeasts were identified based on D1/D2 or ITS gene sequences. Bacterial diversity was widespread, varied and rich among all yeasts except for four strains. Taxa belonging to the phylum Firmicutes, Proteobacteria, Actinobacteria and Bacteroidetes and the genera; Streptococcus, Propionibacterium were common to all the yeasts. Candida tropicalis was used as a model organism to confirm bacteria through fluorescence in situ hybridization (FISH), isolating and re-introducing the isolated bacteria into the yeast. FISH analysis confirmed the endobacteria of C. tropicalis and we have successfully isolated four bacteria only after lysis and disruption of yeast cells. These bacteria were identified as species of Pseudomonas, Chryseobacterium, Lysinibacillus and Propionibacterium. Guestimates indicate 95% of bacterial species of C. tropicalis are yet-to-be-cultivated. We have successfully reintroduced mCherry tagged Pseudomonas into C. tropicalis. Also, auto-fluorescent Prochlorococcus and Rhodopseudomonas could be introduced into C. tropicalis while mCherry tagged E. coli or Salmonella could not be introduced. FISH analysis confirmed the presence of both native and infected bacterial cells present in C. tropicalis. Our findings unveil the insights into the ghost microbiota associated with yeast, which otherwise are considered to be axenic cultures. Their inherent occurrence, together with co-cultivation experiments under laboratory conditions suggests that yeasts are a thriving hub for bacterial communities.
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http://dx.doi.org/10.1038/s41598-021-88658-xDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8087679PMC
April 2021

Corrigendum: Taxogenomics Resolves Conflict in the Genus : A Two and Half Decades Pending Thought to Reclassify the Genus .

Front Microbiol 2020 3;11:1111. Epub 2020 Jun 3.

Department of Plant Sciences, School of Life Sciences, University of Hyderabad, Hyderabad, India.

[This corrects the article DOI: 10.3389/fmicb.2019.02480.].
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http://dx.doi.org/10.3389/fmicb.2020.01111DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7286223PMC
June 2020

Effect of cryopreservation on therapeutic potential of canine bone marrow derived mesenchymal stem cells augmented mesh scaffold for wound healing in guinea pig.

Biomed Pharmacother 2020 Jan 25;121:109573. Epub 2019 Nov 25.

Division of Physiology and Climatology, ICAR-Indian Veterinary Research Institute, Izatnagar, 243 122, U.P., India. Electronic address:

The objective of this study was to compare the therapeutic potential of canine bone marrow derived mesenchymal stem cells (BM MSCs) augmented mesh scaffold for wound healing potential in guinea pig before and after cryopreservation. Bone marrow aspirate was obtained from healthy dogs and culture was expanded in vitro. MSCs augmented mesh scaffold were cryopreserved for 30 days and then used for therapeutic purposes. Both fresh and frozen thaw MSCs augmented mesh scaffold along with fresh MSCs were used for therapeutic purposes in guinea pig. No significant (P > 0.05) difference was observed in population doubling time (PDT) among fresh and frozen thawed BM MSCs. Both fresh and frozen thawed BM MSCs expressed cell surface markers (CD73, CD90, and CD105), and did not express CD34 as was confirmed by Immunocytochemistry and Real-Time Polymerase Chain Reaction. The fresh and frozen thawed BM MSCs successfully differentiated into osteogenic, chondrogenic and adipogenic lineages. Therapeutic results revealed that the percent wound contraction on day 14 was more than 65 % for the mesh augmented with MSCs as well as freshly injected MSCs group as against 33-34 % in the control group. Healed wound quality parameters viz. surface epithelium, neovascularization, and collagen characteristics were better for the mesh augmented with MSCs as well as freshly injected MSCs group compared to the control group. No significant difference was noted among fresh and frozen thawed BM MSCs group and fresh MSCs injected group. Thus, it is concluded from this study that canine BM MSCs augmented mesh scaffold both fresh and frozen thaw can be used for quality wound healing.
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http://dx.doi.org/10.1016/j.biopha.2019.109573DOI Listing
January 2020

Taxogenomics Resolves Conflict in the Genus : A Two and Half Decades Pending Thought to Reclassify the Genus .

Front Microbiol 2019 31;10:2480. Epub 2019 Oct 31.

Department of Plant Sciences, School of Life Sciences, University of Hyderabad, Hyderabad, India.

The genus is taxonomically well studied, and some members are model organisms. However, this genus is comprised of a heterogeneous group of members. 16S rRNA gene-based phylogeny of the genus indicates a motley assemblage of anoxygenic phototrophic bacteria (genus ) with interspersing members of other genera (chemotrophs) making the genus polyphyletic. Taxogenomics was performed to resolve the taxonomic conflicts of the genus using twelve type strains. The phylogenomic analysis showed that spp. can be grouped into four monophyletic clusters with interspersing chemotrophs. Genomic indices (ANI and DDH) confirmed that all the current species are well defined, except . The average amino acid identity values between the monophyletic clusters of members, as well as with the chemotrophic genera, are less than 80% whereas the percentage of conserved proteins values were below 70%, which has been observed among several genera related to . The pan-genome analysis has shown that there are only 1239 core genes shared between the 12 species of the genus . The polyphasic taxonomic analysis supports the phylogenomic and genomic studies in distinguishing the four clusters. Each cluster is comprised of one to seven species according to the current taxonomy. Therefore, to address this taxonomic discrepancy we propose to reclassify the members of the genus into three new genera, gen. nov., gen. nov. and gen. nov., and provide an emended description of the genus . Also, we propose reclassification of as a sub-species of .
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http://dx.doi.org/10.3389/fmicb.2019.02480DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6834548PMC
October 2019

sp. nov., isolated from a yeast ().

Int J Syst Evol Microbiol 2020 Jan;70(1):93-99

Department of Plant Sciences, School of Life Sciences, University of Hyderabad, P.O. Central University, Hyderabad 500046, India.

A Gram-stain-negative, rod shaped, non-motile, aerobic bacterium (strain JC507) was isolated from a yeast ( JY101). Strain JC507 was oxidase- and catalase-positive. Complete 16S rRNA gene sequence comparison data indicated that strain JC507 was a member of the genus and was closely related to NBRC 14944 (98.7 %), followed by CC-VM-7 (98.6 %), ATCC 35910 (98.5 %) and less than 98.5 % to other species of the genus The genomic DNA GC content of strain JC507 was 36.0 mol%. Strain JC507 had phosphatidylethanolamine, four unidentified amino lipids and four unidentified lipids. MK-6 was the only respiratory quinone. The major fatty acids (>10 %) were anteiso-C, iso-C and iso-C3OH. The average nucleotide identity and DNA-DNA hybridization values between strain JC507 and NBRC 14944, CC-VM-7 and ATCC 35910 were 80.2, 83.0 and 87.0 % and 24, 26.7 and 32.7 %, respectively. The results of phenotypic, phylogenetic and chemotaxonomic analyses support the inclusion of strain JC507 as a representative of a new species of the genus for which the name sp. nov. is proposed. The type strain is JC507 (=KCTC 52928=MCC 4072=NBRC 113872).
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http://dx.doi.org/10.1099/ijsem.0.003716DOI Listing
January 2020

Effect of roscovitine on developmental competence of small follicle-derived buffalo oocytes.

Indian J Med Res 2018 12;148(Suppl):S140-S150

Division of Physiology & Climatology, ICAR-Indian Veterinary Research Institute, Bareilly, India.

Background & Objectives: The lower recovery of competent oocytes in buffalo species limits the commercialization of in vitro embryo production technology in field condition. In this context, pre-maturation of small follicle (SF)-derived oocytes with meiotic inhibition may be a promising alternative to obtain more number of competent oocytes. Thus, the present study was conducted with an objective to enhance the developmental potential of less competent SF-derived buffalo oocytes.

Methods: All the visible follicles (used for aspiration) from buffalo ovaries were divided into two categories: large follicle (LF) (follicles having diameter ≥6 mm) and SF (follicles of diameter <6 mm). The competence of LF and SF oocytes was observed in terms of brilliant cresyl blue (BCB) staining, cleavage rate, blastocyst rate and relative gene expression of oocyte and blastocyst competence markers. Thereafter, less competent SF oocytes were treated with 0, 12.5, 25, 50 and 100 mM doses of roscovitine (cyclin-dependent kinase inhibitor) to enhance their developmental potential.

Results: Based on parameters studied, LF oocytes were found to be more competent than SF oocytes. Pre-maturation incubation of SF oocytes with roscovitine reversibly arrested oocyte maturation for 24 h to ensure the proper maturation of less competent oocytes. A significantly higher number of BCB-positive oocytes were noted in roscovitine-treated group than SF group. Cleavage and blastocyst rates were also higher in roscovitine-treated group. The relative messenger RNA expression of oocyte (GDF9, BMP15, GREM1, EGFR, PTGS2 and HAS2) as well as blastocyst (INF-τ, GLUT1 and POU5F1) competence markers was significantly greater in roscovitine-treated group relative to SF group. Again, on comparison with LF group, these parameters depicted a lower value in the treatment group.

Interpretation & Conclusions: The findings of this study has revealed that pre-maturation incubation of SF-derived oocytes with 25 μM roscovitine can improve its developmental competence and thus can be utilized to get maximum number of competent oocytes for better commercialization of in vitro embryo production technology in buffalo.
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http://dx.doi.org/10.4103/ijmr.IJMR_2068_17DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6469368PMC
December 2018

Transcriptome analysis of hopanoid deficient mutant of Rhodopseuodomonas palustris TIE-1.

Microbiol Res 2019 Jan 29;218:108-117. Epub 2018 Oct 29.

Department of Plant Sciences, School of Life Sciences, University of Hyderabad, P.O. Central University, Hyderabad, 500 046, India. Electronic address:

All three domains of life have an ordered plasma membrane which is pivotal in the selective fitness of primitive life. Like cholesterol in eukaryotes, hopanoids are important in bacteria to modulate membrane order. Hopanoids are pentacyclic triterpenoid lipids biosynthesised in many eubacteria, few ferns and lichens. Hopanoid modulates outer membrane order and hopanoid deficiency results in the weakened structural integrity of the membrane which may in turn affect the other structures within or spanning the cell envelope and contributing to various membrane functions. Hence, to decipher the role of hopanoid, genome-wide transcriptome of wild-type and Δshc mutant of Rhodopseudomonas palustris TIE-1 was studied which indicated 299 genes were upregulated and 306 genes were downregulated in hopanoid deficient mutant, representing ∼11.5% of the genome. Thirty-eight genes involved in chemotaxis, response to stimuli and signal transduction were differentially regulated and impaired motility in hopanoid deficient mutant showed that hopanoid plays a crucial role in chemotaxis. The docking study demonstrated that diguanylate cyclase which catalyses the synthesis of secondary messenger exhibited the capability to interact with hopanoids and might be confederating in chemotaxis and signal transduction. Seventy-four genes involved in membrane transport were differentially expressed and cell assays also explicit that the multidrug transport is compromised in Δshc mutant. Membrane transport is reliant on hopanoids which may explain the basis for previous observations linking hopanoids to antibiotic resistance. Disturbing the membrane order by targeting lipid synthesis can be a possible novel approach in developing new antimicrobials and hopanoid biosynthesis could be a potential target.
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http://dx.doi.org/10.1016/j.micres.2018.10.009DOI Listing
January 2019

An allogenic therapeutic strategy for canine spinal cord injury using mesenchymal stem cells.

J Cell Physiol 2019 03 21;234(3):2705-2718. Epub 2018 Aug 21.

Division of Physiology and Climatology, ICAR-Indian Veter inary Research Institute, Izatnagar, Uttar Pradesh, India.

This study was conducted to characterize canine bone marrow-derived mesenchymal stem cells (BMSCs); in vivo tracking in mice, and therapeutic evaluation in canine clinical paraplegia cases. Canine BMSCs were isolated, cultured, and characterized in vitro as per International Society for Cellular Therapy criteria, and successfully differentiated to chondrogenic, osteogenic, and adipogenic lineages. To demonstrate the homing property, the pGL4.51 vector that contained luciferase reporter gene was used to transfect BMSCs. Successfully transfected cells were injected around the skin wound in mice and in vivo imaging was done at 6, 12 and 24 hr post MSCs delivery. In vivo imaging revealed that transfected BMSCs migrated and concentrated predominantly toward the center of the wound. BMSCs were further evaluated for allogenic therapeutic potential in 44 clinical cases of spinal cord injuries (SCI) and compared with conventional therapy (control). Therapeutic potential as evaluated by different body reflexes and recovery score depicted significantly better results in stem cell-treated group compared to control group. In conclusion, allogenic canine BMSCs can serve as potent therapeutic candidate in cell-based therapies, especially for diseases like SCI, where the conventional medication is not so promising.
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http://dx.doi.org/10.1002/jcp.27086DOI Listing
March 2019

Inorganic zinc supplementation modulates heat shock and immune response in heat stressed peripheral blood mononuclear cells of periparturient dairy cows.

Theriogenology 2017 Jun 28;95:75-82. Epub 2017 Feb 28.

Animal Physiology Division, ICAR-National Dairy Research Institute, Karnal, Haryana, India.

Thermal stress in India is one of the major constraints affecting dairy cattle productivity. Every attempt should be made to ameliorate the heat and calving related stress in high producing dairy cows for higher economic returns. In the current study, inorganic zinc was tried to alleviate the adverse effects of thermal stress in periparturient cows. Twelve cows, six each of Sahiwal and Karan Fries (KF) in their second parity with confirmed pregnancy were chosen for the experiment. The blood samples were collected periparturiently on three occasions viz. -21, 0 and +21 days relative to calving. The in vitro study was conducted after isolating peripheral blood mononuclear cells (PBMC) from whole blood. The cultured PBMC were subjected to three different levels of exposures viz. 37°C as control, 42°C to induce thermal stress and 42°C + zinc to ameliorate the adverse effects of high temperature. Heat shock lead to a significant (P<0.05) rise in the level of heat shock proteins (HSP). HSP was more on the day of calving as well. KF showed more HSP concentration than Sahiwal breed indicating the heat bearing capacity of later. Zinc treatment to thermally stressed PBMC caused a fall in the HSP concentration in both the breeds during periparturient period. Moreover, heat stress increased significantly (P<0.05) the Interleukin 6 (IL-6) concentration which declined upon zinc supplementation to PBMC. IL-6 levels decreased periparturiently. Heat and calving related stress caused a fall in the IL-12 levels which increased significantly (P<0.05) with zinc supplementation. These findings suggest that zinc supplementation attenuates the HSP response and augments immunity in PBMC of periparturient dairy cows. The study could help to alleviate the heat stress and potentiate immunity by providing mineral supplements in periparturient dairy cattle habituating tropics.
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http://dx.doi.org/10.1016/j.theriogenology.2017.02.024DOI Listing
June 2017

Impact of Cryopreservation on Caprine Fetal Adnexa Derived Stem Cells and Its Evaluation for Growth Kinetics, Phenotypic Characterization, and Wound Healing Potential in Xenogenic Rat Model.

J Cell Physiol 2017 Aug 1;232(8):2186-2200. Epub 2017 Mar 1.

Division of Physiology and Climatology, ICAR-Indian Veterinary Research Institute, Bareilly, Uttar Pradesh, India.

This study was conducted to know the impact of cryopreservation on caprine fetal adnexa derived mesenchymal stem cells (MSCs) on the basic stem cell characteristics. Gravid caprine uteri (2-3 months) were collected from local abattoir to derive (amniotic fluid [cAF], amniotic sac [cAS], Wharton's jelly [cWJ], and cord blood [cCB]) MSCs and expanded in vitro. Cells were cryopreserved at 3rd passage (P3) using 10% DMSO. Post-thaw viability and cellular properties were assessed. Cells were expanded to determine growth kinetics, tri-lineage differentiation, localization, and molecular expression of MSCs and pluripotency markers; thereafter, these cells were transplanted in the full-thickness (2 × 2cm ) rat skin wound to determine their wound healing potential. The post-thaw (pt) growth kinetics study suggested that cWJ MSCs expanded more rapidly with faster population doubling time (PDT) than that of other fetal adnexa MSCs. The relative mRNA expression of surface antigens (CD73, CD90, and CD 105) and pluripotency markers (Oct4, KLF, and cMyc) was higher in cWJ MSCs in comparison to cAS, cAF, and cCB MSCs post-thaw. The percent wound contraction on 7th day was more than 50% for all the MSC-treated groups (pre and post-thaw), against 39.55% in the control group. On day 28th, 99% and more wound contraction was observed in cAF, cAF-pt, cAS-pt, cWJ, cWJ-pt, and cCB, MSCs with better scores for epithelization, neovascularization, and collagen characteristics at a non-significant level. It is concluded that these MSCs could be successfully cryopreserved without altering their stemness and wound healing properties. J. Cell. Physiol. 232: 2186-2200, 2017. © 2016 Wiley Periodicals, Inc.
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http://dx.doi.org/10.1002/jcp.25731DOI Listing
August 2017

A Comparative Study of Growth Kinetics, In Vitro Differentiation Potential and Molecular Characterization of Fetal Adnexa Derived Caprine Mesenchymal Stem Cells.

PLoS One 2016 3;11(6):e0156821. Epub 2016 Jun 3.

Division of Physiology and Climatology, ICAR-Indian Veterinary Research Institute, Izatnagar-243 122, Bareilly, U.P., India.

The present study was conducted with an objective of isolation, in vitro expansion, growth kinetics, molecular characterization and in vitro differentiation of fetal adnexa derived caprine mesenchymal stem cells. Mid-gestation gravid caprine uteri (2-3 months) were collected from abattoir to derive mesenchymal stem cells (MSCs) from fetal adnexa {amniotic fluid (cAF), amniotic sac (cAS), Wharton's jelly (cWJ) and cord blood (cCB)} and expanded in vitro. These cultured MSCs were used at the 3rd passage (P3) to study growth kinetics, localization as well as molecular expression of specific surface antigens, pluripotency markers and mesenchymal tri-lineage differentiation. In comparison to cAF and cAS MSCs, cWJ and cCB MSCs showed significantly (P<0.05) higher clonogenic potency, faster growth rate and low population doubling (PDT) time. All the four types of MSCs were positive for alkaline phosphatase (AP) and differentiated into chondrogenic, osteogenic, and adipogenic lineages. These stem cells expressed MSC surface antigens (CD73, CD90 and CD105) and pluripotency markers (Oct4, Sox2, Nanog, KLF, cMyc, FoxD3) but did not express CD34, a hematopoietic stem cell marker (HSC) as confirmed by RT-PCR, immunocytochemistry and flow cytometric analysis. The relative mRNA expression of MSC surface antigens (CD73, CD90 and CD105) was significantly (P<0.05) higher in cWJ MSCs compared to the other cell lines. The mRNA expression of Oct4 was significantly (P<0.05) higher in cWJ, whereas mRNA expression of KLF and cMyc was significantly (P<0.05) higher in cWJ and cAF than that of cAS and cCB. The comparative assessment revealed that cWJ MSCs outperformed MSCs from other sources of fetal adnexa in terms of growth kinetics, relative mRNA expression of surface antigens, pluripotency markers and tri-lineage differentiation potential, hence, these MSCs could be used as a preferred source for regenerative medicine.
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http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0156821PLOS
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4892572PMC
August 2017

Nifedipine attenuates the intraocular pressure response to intubation following succinylcholine.

Can J Anaesth 1989 May;36(3 Pt 1):269-72

Department of Anesthesiology, Post-graduate Institute of Medical Education and Research, Chandigarh, India.

Forty patients without eye disease, undergoing elective nonophthalmic surgery, were studied to evaluate the efficacy of sublingual nifedipine in attenuating the intraocular pressure response to succinylcholine administration, laryngoscopy and intubation. The patients were randomly given either nifedipine 10 mg or placebo sublingually 20 minutes before induction of anaesthesia. Intraocular pressure (IOP) and systolic blood pressure (SBP) were recorded before and after induction of anaesthesia. The IOP response to succinylcholine administration, laryngoscopy and intubation was significantly less in patients receiving nifedipine (P less than 0.01). The mean maximum rise in IOP above basal level at one minute post-intubation was 7.82 mmHg in the control group compared with 0.15 mmHg in the nifedipine pre-treated group. These results suggest that sublingual nifedipine is effective in attenuating the IOP response after succinylcholine administration, laryngoscopy and intubation.
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http://dx.doi.org/10.1007/BF03010763DOI Listing
May 1989

Attenuation of pulse rate and blood pressure response to laryngoscopy and tracheal intubation by clonidine.

Int J Clin Pharmacol Ther Toxicol 1988 Jul;26(7):360-3

Department of Anesthesiology, Post-Graduate Institute of Medical Education and Research, Chandigarh, India.

Forty healthy patients (ASA class 1) of both sexes, aged between 20 and 45 years, undergoing routine surgical procedures were included in this double-blind randomized study. They were divided into two groups of 20 each. Patients in group A received no pretreatment, while patients in group B received oral clonidine 5 micrograms kg-1 90 min before induction of anesthesia. All patients received thiopentone (5 mgs kg-1) followed by suxamethonium (1.5 mgs kg-1) to facilitate endotracheal intubation. Control patients showed a significant increase in heart rate and blood pressure; they were significantly lower in the clonidine treated group immediately after intubation (p less than 0.001). The data suggest that the rise in heart rate and blood pressure associated with laryngoscopy and intubation during a routine induction sequence can be attenuated by the use of oral clonidine.
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July 1988
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