Publications by authors named "In Min Hwang"

18 Publications

  • Page 1 of 1

and Antibacterial Activity of Serratamid, a Novel Peptide-Polyketide Antibiotic Isolated from C1, against Phytopathogenic Bacteria.

J Agric Food Chem 2021 May 29;69(19):5471-5480. Epub 2021 Apr 29.

Department of Agricultural Chemistry, Institute of Environmentally Friendly Agriculture, College of Agriculture and Life Sciences, Chonnam National University, Gwangju 61186, Republic of Korea.

A new hybrid non-ribosomal peptide-polyketide antibiotic (serratamid) for phytoprotection was isolated from the ethyl acetate layer of tryptic soy agar culture of the soil bacterium C1 through bioassay-guided fractionation. Its chemical structure was elucidated using instrumental analyses, such as mass and nuclear magnetic resonance spectrometry. Serratamid showed antibacterial activity against 15 phytopathogenic bacteria, with minimum inhibitory concentration (MIC) values ranging from 0.244 to 31.25 μg/mL. , it displayed strong antibacterial activity against and four spp., with MIC values (0.244-0.488 μg/mL) superior to those of streptomycin sulfate, oxolinic acid, and oxytetracycline. Further, serratamid and the ethyl acetate layer of C1 effectively reduced bacterial wilt caused by on tomato seedlings and fire blight caused by on apple fruits in a dose-dependent manner. These results suggest that serratamid is a promising candidate as a potent bactericide for controlling bacterial diseases.
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http://dx.doi.org/10.1021/acs.jafc.1c01162DOI Listing
May 2021

Deciphering the Relationship Between Cycloheximides Structures and Their Different Biological Activities.

Front Microbiol 2021 7;12:644853. Epub 2021 Apr 7.

Department of Agricultural Chemistry, College of Agriculture and Life Science, Institute of Environmentally Friendly Agriculture, Chonnam National University, Gwangju, South Korea.

species are the most important sources of antibacterial, antifungal, and phytotoxic metabolites. In this study, cycloheximide (CH) and acetoxycycloheximide (ACH) were isolated from the fermentation broth of sp. JCK-6092. The antifungal and phytotoxic activities of the two compounds (CH and ACH) and a cycloheximide derivative, hydroxycycloheximide (HCH), were compared. CH exhibited the strongest antagonistic activity against all the true fungi tested, followed by ACH and HCH. However, both CH and ACH displayed similar mycelial growth inhibitory activities against several phytopathogenic oomycetes, and both were more active than that of HCH. Disparate to antifungal ability, ACH showed the strongest phytotoxic activity against weeds and crops, followed by HCH and CH. ACH caused chlorophyll content loss, leaf electrolytic leakage, and lipid peroxidation in a dose-dependent manner. Its phytotoxicity was stronger than that of glufosinate-ammonium but weaker than that of paraquat in the experiments. CH and its derivatives are well-known protein synthesis inhibitors; however, the precise differences between their mechanism of action remain undiscovered. A computational study revealed effects of CHs on the protein synthesis of (oomycetes), (true fungus), and (plant) and deciphered the differences in their biological activities on different targets. The binding energies and conformation stabilities of each chemical molecule correlated with their biological activities. Thus, molecular docking study supported the experimental results. This is the first comparative study to suggest the ribosomal protein alteration mechanisms of CHs in plants and fungi and to thus show how the protein inhibitory activities of the different derivatives are altered using molecular docking. The correlation of structures features of CHs in respect to bond formation with desired protein was revealed by density functional theory. Overall collective results suggested that CHs can be used as lead molecules in the development of more potent fungicides and herbicides molecules.
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http://dx.doi.org/10.3389/fmicb.2021.644853DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8058199PMC
April 2021

Proteomic evaluation of kimchi, a traditional Korean fermented vegetable, and comparison of kimchi manufactured in China and Korea.

J Food Sci Technol 2021 Jan 17;58(1):389-396. Epub 2020 Sep 17.

Hygienic Safety and Analysis Center, World Institute of Kimchi, Gwangju, 61755 Republic of Korea.

Kimchi is a traditional Korean fermented vegetable, which is also widely consumed in Japan and China. However, little is known about the kimchi proteome. In this study, Korean and Chinese kimchi proteomes were evaluated by shotgun proteomics. A total of 250 proteins were annotated, and 29 of these were expressed at > 1% of the average relative abundance. Discrimination of the geographical origins of Korean and Chinese kimchi samples was possible using multivariate analysis of the proteomic data, and 23 proteins were expressed differently between the two types ( < 0.001), and represent possible markers to discriminate between Chinese and Korean kimchi. This study provides important insights into the kimchi proteome and illustrates the proteomic differences caused by geographical origin.
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http://dx.doi.org/10.1007/s13197-020-04777-9DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7813951PMC
January 2021

Distinguishing between long-term-stored and fresh chili pepper powder through fingerprinting of volatiles by headspace capillary-gas chromatography-ion mobility spectrometry.

J Food Sci 2020 Dec 20;85(12):4359-4366. Epub 2020 Nov 20.

Hygienic Safety and Analysis Center, World Institute of Kimchi, 86, Kimchi-ro, Nam-gu, Gwangju, 61755, Republic of Korea.

Long-term storage of chili pepper powder results in physicochemical and microbiological changes that decrease its commercial value; these changes occur owing to fungal growth and production of off-flavor compounds. Herein, long-term-stored chili pepper powder (LSCPP) and fresh chili pepper powder (FCPP) were analyzed using internal transcribed spacer sequencing and volatile organic compound fingerprinting by headspace capillary-gas chromatography-ion mobility spectrometry. Fungal analysis detected only Xeromyces bisporus with high accuracy in all the analyzed LSCPP samples. However, the proliferation of X. bisporus on nonspecific spots complicated the distinguishing process between the two groups based solely on fungal analysis. Therefore, nine compounds (three ketones, one alcohol, two aldehydes, one ester, one furan, and one sulfur compound) obtained by autoxidation and fungal metabolism were selected as potential markers for distinguishing LSCPP and FCPP. These above-mentioned substances, which were confirmed as off-flavor species owing to "stale" odor, emitted lipid fragrance and were used to successfully distinguish LSCPP from FCPP using principal component analysis and linear discriminant analysis. PRACTICAL APPLICATION: According to the research results, it was possible to discriminate between long-term stored and fresh chili pepper powders using nine VOC markers for quality control in industry. In addition, the fungus generated from long-term storage of chili pepper powder was Xeromyces bisporus, which was confirmed to be safe for intake because it does not form secondary toxic metabolites.
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http://dx.doi.org/10.1111/1750-3841.15538DOI Listing
December 2020

Rapid and Simultaneous Quantification of Five Quinolizidine Alkaloids in L. and Its Processed Foods by UPLC-MS/MS.

ACS Omega 2020 Aug 10;5(33):20825-20830. Epub 2020 Aug 10.

Hygienic Safety and Analysis Center, World Institute of Kimchi, Gwangju 61755, Republic of Korea.

Quinolizidine alkaloids (QAs) are toxic secondary metabolites of plants. This study reports the simultaneous quantification of five alkaloids from L. and its processed foods by ultraperformance liquid chromatography with electrospray ionization mass spectrometric detection. After optimizing the extraction conditions, the analytical method was validated for the QAs in lupin beans and in three major processed foods through detection and quantification limits, linearity, precision, and accuracy. The detection and quantification limits of the QAs were 0.5-1.7 and 1.5-5.7 mg kg, respectively. The linearity was greater than 0.9992 and the precision was less than 3.1%. The recoveries of three different concentrations of each QA were 89.5-106.2%. For both raw lupin beans and processed foods, lupanine was the major QA, and 13-hydroxylupanine and angustifoline were detected in lower amounts. This method could be widely used for accurate risk assessment of QAs.
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http://dx.doi.org/10.1021/acsomega.0c01929DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7450503PMC
August 2020

Biorefining Process of Carbohydrate Feedstock (Agricultural Onion Waste) to Acetic Acid.

ACS Omega 2019 Dec 18;4(27):22438-22444. Epub 2019 Dec 18.

R&D Division, World Institute of Kimchi, Gwangju 61755, Republic of Korea.

The biorefining of agricultural waste into green chemicals has clear potential for improving global environmental sustainability. In this study, we evaluated the potential of acetic acid production from carbohydrate feedstock (onion waste, OW) as a more environmentally friendly source than feedstock produced from natural gas. In particular, OW is an ideal feedstock for the biorefining process as it contains a sufficient amount of carbohydrates (69.7%). Five days of the simultaneous saccharification and two-step fermentation (SSTF) process produced acetic acid from OW more efficiently than the simultaneous saccharification and cofermentation (SSCF) process. SSTF produced 19.3 g/L acetic acid and recorded the highest conversion yield (90.5%) from OW (6% substrate loading, w/v). These results suggested that acetic acid can be efficiently and sustainably produced from OW by the SSTF process.
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http://dx.doi.org/10.1021/acsomega.9b03093DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6941178PMC
December 2019

Characterization of natural gums via elemental and chemometric analyses, synthesis of silver nanoparticles, and biological and catalytic applications.

Int J Biol Macromol 2020 Mar 15;147:853-866. Epub 2019 Nov 15.

School of Materials and Mineral Resources Engineering, Engineering Campus, Universiti Sains Malaysia, Nibong Tebal, Pulau Pinang 14300, Malaysia.

Gums; composed of polysaccharides, carbohydrates, proteins, and minerals, are high molecular weight hydrophilic compounds with several biological applications. This study describes the nutritional and toxic elements content, chemical composition, synthesis of silver nanoparticles (G-AgNPs), and pharmacological and catalytic properties of Prunus armeniaca (apricot), Prunus domestica (plums), Prunus persica (peaches), Acacia modesta (phulai), Acacia arabica (kikar), and Salmalia malabarica (silk cotton tree) gums. The elemental contents were analyzed by inductively coupled plasma-optical emission spectroscopy (ICP-OES) and ICP-mass spectrometry (ICP-MS). NMR spectroscopy was used for the identification of class of compounds in the mixture, their functional groups were determined through FTIR techniques, and plasmon resonance and size of G-AgNPs through UV-Vis spectroscopic technique and transmission electron microscopy (TEM). From the results, nutritional elements were present at appreciable concentrations, whereas toxic elements showed content below the maximum permissible ranges. Using the elemental data, linear discriminant and principal component analyses classified the gums to 99.9% variability index. Furthermore, G-AgNPs exhibited significant antioxidant, antibacterial, and redox catalytic potential. Hence, the subject G-AgNPs could have promising nutritional, therapeutic and environmental remediation applications.
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http://dx.doi.org/10.1016/j.ijbiomac.2019.09.245DOI Listing
March 2020

Advanced strategy to produce insecticidal destruxins from lignocellulosic biomass .

Biotechnol Biofuels 2019 25;12:188. Epub 2019 Jul 25.

R&D Division, World Institute of Kimchi, 86 Kimchi-ro, Nam-gu, Gwangju, 61755 Republic of Korea.

Background: Biorefineries are widely recognized as the most feasible solution to the problem of achieving environmental sustainability along with economic growth. Furthermore, pine wilt disease has caused severe environmental and economic damage worldwide to date. Herein, a highly efficient, advanced process for producing destruxins (DTXs) from (MCT) is reported, along with an application strategy.

Results: The acetic acid-sodium chlorite pretreatment of MCT (AASC-MCT) is found to improve the monosaccharide production. Through biocatalytic conversion processes (simultaneous saccharification and cultivation), JEF-279 can efficiently produce DTXs from 1% (w/v) AASC-MCT, i.e., DTX E (334.8 mg/L), A (288.8 mg/L), and B (48.6 mg/L). (MA, Japanese pine sawyer) is known to act as a mediator transferring to pinewood. As is associated with the occurrence of pine wilt disease, biological control of MA is a major strategy or controlling this disease. In this study, upon the application of a mixture of DTXs and protease-containing culture filtrate (PCF), complete mortality of MA is observed after a 5-day incubation. The MA immune system response is believed to cause an overexpression of actin and tropomyosin as a defense mechanism against the flaccid paralysis induced by the DTXs and PCF treatment.

Conclusions: These results suggest that MCT can be used as a major feedstock in the biorefinery industry and that DTXs can be applied as an insecticide for biological control of pine wilt disease via MA termination.
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http://dx.doi.org/10.1186/s13068-019-1530-8DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6657178PMC
July 2019

Dietary intake assessment of macro, trace, and toxic elements via consumption of kimchi in South Korea.

J Sci Food Agric 2019 Nov 13;99(14):6474-6481. Epub 2019 Aug 13.

World Institute of Kimchi, Gwangju, Republic of Korea.

Background: Kimchi, a Korean food, is now produced and consumed in many other countries. In this study, the macro, trace, and toxic element content in varieties of kimchi from Korea and China was evaluated. A health-risk assessment, involving the calculation of estimated dietary intake values, was undertaken. To discriminate among samples with different origins, linear discriminant analysis (LDA) and principal component analysis (PCA) were applied.

Results: The analytical methods used for analyses were successfully validated. In the quantification of elements, no significant differences were detected in the concentrations of the elements that were analyzed. Based on the fresh weight of kimchi, among the macro elements, the concentrations of Na and K were relatively high, with values of 4181.9-9919.8 mg kg and 1661.8-6623.5 mg kg , respectively. Among the trace elements that were analyzed, zinc in Korean samples, and strontium in Chinese samples showed the highest concentrations, at 2.67 and 2.98 mg kg , respectively. Concentration levels of the toxic trace elements were within permissible limits. The amount of daily intake and provisional daily intake of each element calculated based on kimchi intake confirmed its safety for consumption. Linear discriminant analysis and PCA successfully discriminated among the samples of Chinese and Korean origin.

Conclusion: The provisional daily intake of the toxic elements was within provisional tolerable daily intake values. The normal intake of kimchi could not pose a threat upon consumption. © 2019 Society of Chemical Industry.
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http://dx.doi.org/10.1002/jsfa.9926DOI Listing
November 2019

Simultaneous direct determination of 15 glucosinolates in eight Brassica species by UHPLC-Q-Orbitrap-MS.

Food Chem 2019 Jun 16;282:127-133. Epub 2018 Dec 16.

Hygienic Safety and Analysis Center, World Institute of Kimchi, Gwangju 61755, Republic of Korea. Electronic address:

Glucosinolates (GLS) have been reported to have significant anti-oxidative, antimicrobial, and anti-cancer activities. The current study was aimed to develop an analytical method for glucosinolate quantitation in eight Brassica species from Gwangju, Republic of Korea. For this purpose the UHPLC-Q-Orbitrap-MS technique was used and validated for optimal extraction conditions, detection and quantitation limits, linearity, precision, and accuracy. According to the results of GLS profiling, the total GLS concentration decreased in the order of cabbage > broccoli > cauliflower > mustard > kimchi cabbage > young radish ∼ kale. All Brassica species contained glucoerucin (GER) and glucobrassicin (GBR) as major GLS with the high levels in cabbage (5.913 μM/g) and broccoli (1.723 μM/g), respectively. The contents of minor GLS were species-dependent, and could therefore be used for Brassica species classification.
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http://dx.doi.org/10.1016/j.foodchem.2018.12.036DOI Listing
June 2019

Effective approach to organic acid production from agricultural kimchi cabbage waste and its potential application.

PLoS One 2018 20;13(11):e0207801. Epub 2018 Nov 20.

R&D Division, World Institute of Kimchi, Gwangju, Republic of Korea.

The biotransformation of agricultural waste into valuable chemicals represents a promising approach in the field of biorefining. Herein, a general but highly efficient and robust process is reported for the production of organic acid from kimchi cabbage waste using lactic acid bacteria. The organic acid produced was tested for efficacy as a biological control agent. Lactobacillus sakei WiKim31 and L. curvatus WiKim38 could efficiently produce organic acids including lactic acid (12.1 and 12.7 g/L), fumaric acid (7.4 and 7.1 g/L), and acetic acid (4.5 and 4.6 g/L) from kimchi cabbage waste (3% substrate loading, w/v) by simultaneous saccharification and fermentation processes for 48 h, and the culture filtrate induced complete mortality of J2s Meloidogyne incognita at 2.5% concentration. These results suggested that lactic acid bacteria L. sakei WiKim31 and L. curvatus WiKim38 can efficiently produce organic acids, and the culture filtrate can be applied as a microbial nematicide.
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http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0207801PLOS
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6245790PMC
April 2019

Determination of macro, micro, trace essential, and toxic elements in Garcinia cambogia fruit and its anti-obesity commercial products.

J Sci Food Agric 2019 Mar 13;99(5):2455-2462. Epub 2018 Dec 13.

Department of Food and Nutrition, Chosun University, Gwangju, Republic of Korea.

Background: Garcinia (Clusiaceae) species are traditionally used as flavoring agents in curries and to cure several human health complications. This study investigated 31 macro, micro, and trace elements in microwave-assisted digested samples of Garcinia cambogia fruit and its anti-obesity commercial products by inductively coupled plasma-optical emission spectroscopy (ICP-OES) and inductively coupled plasma-mass spectrometric (ICP-MS) techniques. The methods were also validated using the coefficient of determination (R ), limits of detection and quantification (LOD, LOQ), precision (CV%), analysis of certified reference materials, spiking recovery experiments, and participation in an accredited laboratory proficiency test organized by Food Analysis Performance Assessment Scheme (FAPAS).

Results: Quality assurance confirmed that the methods were efficient and in accordance with criteria set by the Association of Official Analytical Chemists (AOAC). In the elemental analysis, the analyzed macro, micro, and trace essential elements were present in appreciable concentrations, which could meet the human nutritional requirements. Traces of toxic elements were within safe limits.

Conclusion: From the results of the current study, the fruit and its commercial products could be considered potential sources of mineral elements without posing any threats to consumers. © 2018 Society of Chemical Industry.
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http://dx.doi.org/10.1002/jsfa.9454DOI Listing
March 2019

Determination of macro, micro and trace elements in citrus fruits by inductively coupled plasma-optical emission spectrometry (ICP-OES), ICP-mass spectrometry and direct mercury analyzer.

J Sci Food Agric 2019 Mar 5;99(4):1870-1879. Epub 2018 Nov 5.

Department of Food and Nutrition, Chosun University, Gwangju, Republic of Korea.

Background: Citrus fruits are widely consumed around the world. Several elements are known to have important physiological functions in living things, whereas others are reported to have toxic effects. This study was aimed to analyze the concentration of macro, micro and trace elements in citrus fruit varieties. Samples were acid digested using a microwave system and analyzed for macro elements by inductively coupled plasma-optical emission spectrometry (ICP-OES) and for micro and trace elements by inductively coupled plasma-mass spectrometry (ICP-MS). The application of analytical techniques was analyzed by determining method validation parameters including limits of detection and quantification, linearity, precision, spiking experiments and analyzing certified reference material (NIST CRM-1570a), Spinach leaves.

Results: The elemental levels of citrus fruits were found largely to be dependent upon the type of analyzed samples. Among nutritionally important elements, citrus fruits were good sources of potassium (95.13-270.4 mg kg ), calcium (10.57-75.29 mg kg ), zinc (466.5-1611 µg kg ) and manganese (35.35-1902 µg kg ). The concentrations of toxic elements (Pb, Cd, As, Al, Hg) were very low.

Conclusion: In general, the concentrations of analyzed elements were within the critical levels specified by the Food and Agriculture Organization/World Health Organization and Food and Nutrition Board USA. Linear discriminant analysis successfully separated the samples into clear groups with 93.9% correct classification. © 2018 Society of Chemical Industry.
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http://dx.doi.org/10.1002/jsfa.9382DOI Listing
March 2019

Geographical origin authentication of pork using multi-element and multivariate data analyses.

Meat Sci 2017 Jan 28;123:13-20. Epub 2016 Aug 28.

Department of Food and Nutrition, Chosun University, Gwangju 61452, Republic of Korea. Electronic address:

In the recent years, consumers have become increasingly concerned about the authenticity and labeling claims associated with meat and meat based products. In this study, investigating geographical origin authenticity of pork, 323 samples of pork belly were collected from Korea, USA, Germany, Austria, Netherlands and Belgium. These were analyzed for twenty-nine macro and trace elements using inductively coupled plasma-optical emission spectroscopy (ICP-OES), and ICP-mass spectrometry (MS). The applied analytical techniques were validated by quality assurance parameters in which the values of correlation coefficient, limits of detection and quantification, precision, and spiking recovery confirmed that the methods were well efficient and in accordance to the criteria set by the Association of Official Analytical Chemists (AOAC) for metals analysis. From the results of multivariate analyses, it was found that the trace elements are promising constituents which could be used to accurately determine the inter-continental provenance of pork.
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http://dx.doi.org/10.1016/j.meatsci.2016.08.011DOI Listing
January 2017

Elemental profiling and geographical differentiation of Ethiopian coffee samples through inductively coupled plasma-optical emission spectroscopy (ICP-OES), ICP-mass spectrometry (ICP-MS) and direct mercury analyzer (DMA).

Food Chem 2016 Dec 31;212:512-20. Epub 2016 May 31.

Department of Food and Nutrition, Chosun University, Gwangju 61452, Republic of Korea. Electronic address:

This study was aimed to establish the elemental profiling and provenance of coffee samples collected from eleven major coffee producing regions of Ethiopia. A total of 129 samples were analyzed for forty-five elements using inductively coupled plasma (ICP)-optical emission spectroscopy (OES), ICP-mass spectrometry (MS) and direct mercury analyzer (DMA). Among the macro elements, K showed the highest levels whereas Fe was found to have the lowest concentration values. In all the samples, Ca, K, Mg, P and S contents were statistically significant (p<0.05). Micro elements showed the concentrations order of: Mn>Cu>Sr>Zn>Rb>Ni>B. Contents of the trace elements were lower than the permissible standard values. Inter-regions differentiation by cluster analysis (CA), linear discriminant analysis (LDA) and principal component analysis (PCA) showed that micro and trace elements are the best chemical descriptors of the analyzed coffee samples.
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http://dx.doi.org/10.1016/j.foodchem.2016.05.178DOI Listing
December 2016

Determination of minor and trace elements in aromatic spices by micro-wave assisted digestion and inductively coupled plasma-mass spectrometry.

Food Chem 2014 Sep 1;158:200-6. Epub 2014 Mar 1.

Department of Food and Nutrition, Chosun University, Gwangju 501-759, Republic of Korea. Electronic address:

This study aimed at analyzing the concentrations of 23 minor and trace elements in aromatic spices by inductively coupled plasma-mass spectrometry (ICP-MS), after wet digestion by microwave system. The analytical method was validated by linearity, detection limits, precision, accuracy and recovery experiments, obtaining satisfactory values in all cases. Results indicated the presence of variable amounts of both minor and trace elements in the selected aromatic spices. Manganese was high in cinnamon (879.8 μg/g) followed by cardamom (758.1 μg/g) and clove (649.9 μg/g), strontium and zinc were high in ajwain (489.9 μg/g and 84.95 μg/g, respectively), while copper was high in mango powder (77.68 μg/g). On the whole some of the minor and essential trace elements were found to have good nutritional contribution in accordance to RDA. The levels of toxic trace elements, including As, Cd, and Pb were very low and did not found to pose any threat to consumers.
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http://dx.doi.org/10.1016/j.foodchem.2014.02.103DOI Listing
September 2014

Analysis of minor and trace elements in milk and yogurts by inductively coupled plasma-mass spectrometry (ICP-MS).

Food Chem 2014 Mar 9;147:220-4. Epub 2013 Oct 9.

Department of Food and Nutrition, Chosun University, Gwangju 501-759, Republic of Korea.

This study aimed at analysing the content of 22 minor and trace elements in milk and yogurts from South Korea, using inductively coupled plasma-mass spectrometry (ICP-MS), after digestion using heating block. The analytical method was validated by detection limits, precision, accuracy and recovery experiments, obtaining satisfactory results in all cases. Results of both minor and trace elements were found mostly similar in all varieties of milk and yogurts studied. Copper and manganese were comparatively high in fruit mixed yogurts, while selenium was high in milk samples. The overall concentrations of minor elements were found to have good nutritional values in accordance to RDA. Zn showed the highest concentration, followed by Rb, Se, Sr and Cr. The level of toxic trace elements, including As, Cd and Pb were very low and did not pose any threat to consumers.
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http://dx.doi.org/10.1016/j.foodchem.2013.09.147DOI Listing
March 2014

Method validation for simultaneous determination of chromium, molybdenum and selenium in infant formulas by ICP-OES and ICP-MS.

Food Chem 2013 Dec 22;141(4):3566-70. Epub 2013 Jun 22.

Department of Food and Nutrition, Chosun University, Gwangju 501-759, Republic of Korea.

This study aimed to validate the analytical method for simultaneous determination of chromium (Cr), molybdenum (Mo), and selenium (Se) in infant formulas available in South Korea. Various digestion methods of dry-ashing, wet-digestion and microwave were evaluated for samples preparation and both inductively coupled plasma optical emission spectrometry (ICP-OES) and inductively coupled plasma mass spectrometry (ICP-MS) were compared for analysis. The analytical techniques were validated by detection limits, precision, accuracy and recovery experiments. Results showed that wet-digestion and microwave methods were giving satisfactory results for sample preparation, while ICP-MS was found more sensitive and effective technique than ICP-OES. The recovery (%) of Se, Mo and Cr by ICP-OES were 40.9, 109.4 and 0, compared to 99.1, 98.7 and 98.4, respectively by ICP-MS. The contents of Cr, Mo and Se in infant formulas by ICP-MS were found in good nutritional values in accordance to nutrient standards for infant formulas CODEX values.
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http://dx.doi.org/10.1016/j.foodchem.2013.06.034DOI Listing
December 2013