Prof. Imad Fadl Elmula, MD, PhD. - Assafa Academy - Prof.

Prof. Imad Fadl Elmula

MD, PhD.

Assafa Academy

Prof.

Khartoum, Khartoum | Sudan

Main Specialties: Urology

Additional Specialties: Clinical Genetics and Urology


Top Author

Prof. Imad Fadl Elmula, MD, PhD. - Assafa Academy - Prof.

Prof. Imad Fadl Elmula

MD, PhD.

Introduction

1984 - 1985 Internship at Khartoum North and Elobied Hospitals.
1985 - 1986 Hardship period at Kadugly Hospital, Kadugly
1986 - 1987 Department of Surgery, Elobied Hospital
1987 - 1990 Department of Urology, Ibin Sina Hospital
1990 - 1993 Department of Surgical Trauma, Rashid hospital, Dubai
1993 - 2000 Department of Urology, Helsingborg hospital, Helsingborg
1996 - 2002 Department of Clinical Genetics, Lund University hospital, Lund
2002 - 2005 Founder and in-charge of the department of clinical genetics, the radiation and isotopes center.
2003-2005 Founder and Director Al Neelain Medical Research Center, Al Neelain University
2004 - 2006 Department of Urology, Khartoum teaching hospital
2004 - 2007 General director Al Neelain University Medical research Center
2004 - 2005 Director of the School of basic science, Faculty of medicine, Al Neelain University
2002 - 2006 Department of pathology, faculty of medicine, Al Neelain University
2006 Promoted full professor, Al Neelain University
2005 - 2007 Deputy Dean Faculty of Medical Laboratory Sciences, Al Neelain University
2007 - 2009 Department of Urology, Soba University hospital, Sudan-Khartoum
2006 - Today Consultant Cytogenetcisit, Elite Center, Khartoum-Sudan
2010 - 2011 Department of Urology, Khartoum Teaching Hospital, Sudan
2007 - 2011 Dean Faculty of Medical Laboratory Sciences- Al Neelain University
2011- 2014 Dean Faculty of Medicine and Health Sciences, Al Neelain University
2011 – Present Consultant Urologist, Bashair University Hospital, Khartoum, Sudan
2014- 1015 Secretary of the Academic Affairs and Coordinator of the Program of the Medical Laboratory Sciences, Nahda College
2016- Present Dean of Assafa Academy

Primary Affiliation: Assafa Academy - Khartoum, Khartoum , Sudan

Specialties:

Additional Specialties:

Research Interests:


View Prof. Imad Fadl Elmula’s Resume / CV

Metrics

Number of Publications

36

Publications

Number of Profile Views

1250

Profile Views

Number of Article Reads

155

Reads

Number of Citations

79

Citations

Education

Mar 2017
Faculty of medicine, Lund University, Lund
Doctor of Philosophy (Ph.D)
Mar 1998
Health and Welfare, Stockholm
Swedish Board of Urology
Mar 1997
Clinical Diploma in Renal Transplant Surgery Malmö University Hospital, Malmo
Malmö University Hospital, Malmo
Mar 1997
Lund University hospital, Lund, Sweden
Clinical Diploma in Pediatric Surgical Urology
Mar 1984
Faculty of Medicine, University of Craiova, Craiova
MD

Publications

36Publications

155Reads

79PubMed Central Citations

The Impact of Monoclonal Origin and Intraluminal Seeding Mechanism on Management of Non-Muscle Invasive Bladder Cancer: Needs for More Preoperative Adjuvant Intravesical Immune-and/or Chemo-Therapy

Clin Oncol. 2018; 3: 1420

Clinics in Oncology

Bladder cancer is a common disease, worldwide it is ranked as the seventh and the seventeenth most frequent cancer worldwide in men and women respectively. Based on the histopathology (grading and staging), bladder cancer can be classifed into non-muscle invasive (superfcial) and muscleinvasive disease. Te natural history of the superfcial tumors differs from the muscle invasive ones. Most non-muscle invasive tumors are characterized by multiplicity, high recurrence rate, and heterogeneous natural history. According to many investigators, the multifocal nature of nonmuscle invasive uroepithelial cancer, together with a propensity for recurrence (polychrono topicity) is the results of intraluminal seeding of viable detached malignant cells that results in genetically monoclonal multiple tumors. Tus, the intraluminal shedding and implantation of viable tumor cells have been proposed as the mechanisms responsible for both synchronous and metachronous multifocal bladder tumors. Tese fndings are of considerable relevance for therapeutic strategies, suggesting that complete endoscopic removal of the primary tumor is ofen not enough to treat even non-muscle invasive bladder cancer because by, or during, the time of initial treatment, several micro satellite tumors are already implanted in the bladder. Terefore, additional measures to prevent tumor cell seeding and growth of the already implanted ones may reduce the recurrence rate of non-muscle invasive bladder cancer. Te intraluminal seeding mechanism of synchronous and/or metachronous tumors enforces more emphasis on intravesical adjuvant therapy in superfcial bladder cancer, including the use of intravesical chemo- or immunotherapy and long interval follow-up for those patients. Adopting such therapeutic strategy may improve morbidity and mortality of superfcial bladder cancer.

View Article
March 2018
3 Reads

Sexually transmitted infections other than HIV/AIDS among women of low socio-economic class attending antenatal clinics in Khartoum, Sudan.

Int J STD AIDS 2017 07 31;28(8):781-787. Epub 2016 Aug 31.

4 Department of Medicine, Faculty of Medicine, Sudan Medical and Scientific Research Institute, University of Medical Sciences and Technology, Khartoum, Sudan.

View Article
July 2017
12 Reads
1.040 Impact Factor

Awareness and Attitudes of Nursing Students towards Prevention of Cervical Cancer

AbdAllah AAA, Hummeida ME, Elmula IMF (2016)

Cervical Cancer:

Cervical cancer is the second most common women cancer worldwide, accounting for 13% of female cancers. It ranks as the second most frequent cancer among women after breast cancer in Sudan as well as other developing countries. This study seeks to assess the knowledge, and the attitude of female staff and students of undergraduate nursing students of Khartoum universities, Sudan, towards cervical cancer prevention. A structured interview questionnaire was used for data collection, the questions were made to capture the objectives of the study. Most of the students (84.0%) were not aware of other screening methods than Pap smear test and the burden of cervical cancer and its prevention (p = 0.000). Approximately half of the respondents (49.9%) have no complete information about the HPV vaccine. However, the other half showed positive attitudes towards HPV vaccination and were eager to recommend HPV vaccine to their family and other members of the community. More than two thirds of respondents exhibited positive attitude and were willing to undergo Pap smear in the future. The study shows that the majority of the participants have poor information about cervical cancer prevention. Education would motivate nurses to participate actively in awareness raising, screening, and management

View Article
May 2017
4 Reads

Maternal Serum Triple Screening Test and Pregnancy Outcome in Elderly Sudanese Pregnant Ladies in Khartoum State-Sudan

Pathology and Laboratory Medicine 2017; 1(2): 54-60

Pathology and Laboratory Medicine

The possibility to deliver infants with congenital defects or diseases is thought to be associated with increased in maternal age. This fact is well established and documented in previous studies done in the United States of America. Infants with birth defects is not the only disastrous outcome, pregnancy and delivery complications, abortion, stillbirth are also known consequences. The aim of this study, to detect a possible association between increased maternal age, and Triple Screening Test results. And compare the findings of the Triple Screening Test results with abnormal pregnancy outcome among Sudanese pregnant ladies. Study performed in ninety-one pregnant ladies accepted to participate. Participants were assigned to two groups; study group including 69 (75.8%) women equal to and above 35 years and control group including 22 (24.2%) women less than 30 years. Structured questionnaire, clinical examinations including ultra-sound for the determination of gestational age were also performed. Blood samples were collected from all participants during the 14th to 20th week of gestational age. Sera were separated and used for the determination of human chorionic gonadotropin, alpha fetoprotein, and free estriol concentrations using standardized quantitative methods (ELISA) collectively known as the Triple Screening Test. In the study group, participants delivered 57 (82.6%) normal healthy infants. Twelve ladies (17.4%) delivered infants with adverse dimorphic features. The defects varied; four (5.8%) had stillbirth, four (5.8%) had abortion at the second trimester, three (4.3%) had premature delivery, and one (1.4%) had intrauterine death. And matching control group, none of the participants (0%) give birth to an infant with congenital defect. Highly significant positive correlation was detected between increase in maternal age and abnormal pregnancy outcome. The Triple Screening Test against the pregnancy outcome in study group, showed statistically significant differences between levels of β-HCG, AFP, and Free estriol and pregnancy outcomes with Pvalues of 0.000, 0.5240, and 0.000 were obtained using Pearson Chi-squire test of significance, respectively. Study conclude that abnormal pregnancy outcome is strongly associated with increase in maternal age. Also there were strongly association between the Triple Screening Test and abnormal pregnancy outcome.

View Article
March 2017
6 Reads

Sexually transmitted infections other than HIV/AIDS among women of low socio-economic class attending antenatal clinics in Khartoum, Sudan

International Journal of STD & AIDS

Sexually transmitted infections (STIs) are major health threats affecting people globally; however, the burden of STIs is greatest in low-income countries. Since they are physiologically more vulnerable, women are mostly affected. The risk is increased dramatically during pregnancy leading to serious health complications that may affect the newborn. Underprivileged pregnant women attending antenatal clinics for routine checkups in displaced camps, a women’s prison and several peripheral health centres were clinically and laboratory screened for trichomoniasis, chlamydial infections, gonorrhea and syphilis. A total of 426 women with an age range of 14-45 years were included. Clinical data, blood, cervical and vaginal swabs were collected. Conventional bacteriological and serological methods were applied. All attendees were HIV1/2-negative. The prevalence of Trichomonas vaginalis, Chlamydia trachomatis, Neisseria gonorrhoeae and Treponema pallidum infections was found to be 7.8%, 4.9%, 0% and 5%, respectively. Although vaginal discharge, among other symptoms, is known to be the most significant indicator for STIs, our identified positive predictive value was only 14.1%. We conclude that use of syndromic approach for diagnosing and treating attendees of antenatal settings is of low clinical value and many easily curable STIs will be overlooked. Consequently, trichomoniasis, chlamydial infection and syphilis prevailed widely among this population

View Article
July 2016
2 Reads

Status of serum magnesium in Sudanese patients with cardiac syndrome X

Neelain Medical Journal

Background:Cardiac syndrome X (angina chest pain, positive stress - ECG, and normal coronary angiogram) has serious medical complications. Magnesium may be an important factor associated with the disease. The present study aimed to examine the association between serum magnesium and cardiac syndrome X (CSX) in Sudanese patients. Materials and methods:A total of 50 patients with CSX and their matching control were enrolled in this study, 4 ml of venous blood were collected and placed in plain containers, centrifuged and used for determination of serum magnesium. Magnesium quantified by using a calmagite method. Data were entered to the computer and analysed by using SPSS. Results:Median (75th -25th quartile) of the serum magnesium showed no significant difference between the patients with CSX and their well-matched control [2.4(1.9-2.8) vs. 2.5 (1.9-3.2) mg/dl;P= 0.150]. Direct correlation was observed between serum magnesium and urea [r= 0.214;P=0.032]. Conclusion serum magnesium was not associated with cardiac syndrome X in this setting

View Article
June 2016
4 Reads

Extra virgin olive oil potentiates the effects of aromatase inhibitors via glutathione depletion in estrogen receptor-positive human breast cancer (MCF-7) cells.

Food Chem Toxicol 2013 Dec 23;62:817-24. Epub 2013 Oct 23.

Department of Pathology, Faculty of Medicine and Health Sciences, Al-Neelain University, 12702 Khartoum, Sudan; Department of Biochemistry, Faculty of Science and Technology, Al-Neelain University, 12702 Khartoum, Sudan. Electronic address:

View Article
December 2013
15 Reads
3 Citations
2.900 Impact Factor

Frequency of BCR-ABL fusion transcripts in Sudanese patients with chronic myeloid leukemia using real-time reverse transcription-polymerase chain reaction.

Saudi Med J 2013 Jan;34(1):29-33

Department of Hematology and Blood Transfusion, Faculty of Medical Laboratory Sciences, Alzaiem Alazhari University, Khartoum, Sudan.

View Article
January 2013
4 Reads
1 Citation
0.550 Impact Factor

Significance of serum total prostate specific antigen and digital rectal examination in the diagnosis of prostate cancer.

Saudi Med J 2011 Nov;32(11):1133-6

Department of Clinical Chemistry, Faculty of Medical Laboratory Sciences, Al Neelain University, Khartoum, Sudan.

View Article
November 2011
9 Reads
0.550 Impact Factor

Age-specific reference range for serum prostate-specific antigen in Sudanese men.

Saudi Med J 2011 Sep;32(9):930-2

Department of Clinical Chemistry, Faculty of Medical Laboratory Sciences, Al-Neelain University, PO Box 12702, Postal Code 11121, Khartoum, Sudan.

View Article
September 2011
5 Reads
2 Citations
0.550 Impact Factor

Tuberculosis in Sudan: a study of Mycobacterium tuberculosis strain genotype and susceptibility to anti-tuberculosis drugs.

BMC Infect Dis 2011 Aug 16;11:219. Epub 2011 Aug 16.

Al Neelain University, Khartoum, P. O. Box 12702, 11121 Khartoum, Sudan.

View Article
August 2011
10 Reads
14 Citations
2.610 Impact Factor

Frequencies of BCR-ABL1 fusion transcripts among Sudanese chronic myeloid leukaemia patients.

Genet Mol Biol 2010 Apr 1;33(2):229-31. Epub 2010 Jun 1.

Department of Molecular Biology, Institute of Endemic Diseases, University of Khartoum, Khartoum Sudan.

View Article
April 2010
5 Reads
3 Citations
1.200 Impact Factor

A case of Cornelia de Lange syndrome from Sudan.

BMC Pediatr 2007 Jan 29;7. Epub 2007 Jan 29.

The Orchids Organization for Children with Special Needs, Khartoum, Sudan.

View Article
January 2007
5 Reads
3 Citations
1.920 Impact Factor

A del(X)(p11) carrying SRY sequences in an infant with ambiguous genitalia.

BMC Pediatr 2006 Apr 4;6:11. Epub 2006 Apr 4.

Institute of Endemic Diseases, University of Khartoum, Khartoum, Sudan.

View Article
April 2006
4 Reads
2 Citations
1.920 Impact Factor

Female genital mutilation of a karyotypic male presenting as a female with delayed puberty.

BMC Womens Health 2006 Mar 29;6. Epub 2006 Mar 29.

Institute of Endemic Diseases, University of Khartoum, Khartoum, Sudan.

View Article
March 2006
7 Reads
2 Citations
1.660 Impact Factor

Chromosomal changes in uroepithelial carcinomas.

Authors:
Imad Fadl-Elmula

Cell Chromosome 2005 Aug 7;4. Epub 2005 Aug 7.

Al Neelain Medical Research Center, Faculty of Medicine, Al Neelain University, Khartoum, Sudan.

View Article
August 2005
3 Reads
12 Citations

[Sudanese cytogeneticists--vision of a new kind of assistance].

Lakartidningen 2004 Feb;101(8):702-5

Avdelningen för klinisk genetik, Universitetssjukhuset i Lund.

View Article
February 2004
9 Reads

Chromosomal abnormalities in inflammatory pseudotumor of the urinary bladder.

Cancer Genet Cytogenet 2003 Jun;143(2):169-71

Department of Surgical Urology, Faculty of Medicine, Upper Nile University, Upper Nile State, Sudan.

View Article
June 2003
4 Reads

Chromosomal aberrations in benign and malignant bilharzia-associated bladder lesions analyzed by comparative genomic hybridization.

BMC Cancer 2002 Mar 22;2. Epub 2002 Mar 22.

Department of Clinical Genetics, University Hospital, SE-22185 Lund, Sweden.

View Article
March 2002
9 Reads
4 Citations
3.360 Impact Factor

Identification of cytogenetic subgroups and karyotypic pathways in transitional cell carcinoma.

Cancer Res 2001 Nov;61(22):8241-6

Department of Clinical Genetics, University Hospital, SE-221 85 Lund, Sweden.

View Article
November 2001
4 Reads
11 Citations
9.330 Impact Factor

Characterization of chromosomal abnormalities in uroepithelial carcinomas by G-banding, spectral karyotyping and FISH analysis.

Int J Cancer 2001 Jun;92(6):824-31

Department of Clinical Genetics, University Hospital, Lund, Sweden.

View Article
June 2001
9 Reads
2 Citations
5.080 Impact Factor

Karyotypic characterization of urinary bladder transitional cell carcinomas.

Genes Chromosomes Cancer 2000 Nov;29(3):256-65

Department of Clinical Genetics, University Hospital, Lund, Sweden.

View Article
November 2000
2 Reads
6 Citations
4.040 Impact Factor

Cytogenetic analysis of upper urinary tract transitional cell carcinomas.

Cancer Genet Cytogenet 1999 Dec;115(2):123-7

Department of Clinical Genetics, University Hospital, Lund, Sweden.

View Article
December 1999
7 Reads
2 Citations

Cytogenetic monoclonality in multifocal uroepithelial carcinomas: evidence of intraluminal tumour seeding.

Br J Cancer 1999 Sep;81(1):6-12

Department of Clinical Genetics, University Hospital, Lund, Sweden.

View Article
September 1999
11 Reads
8 Citations
4.840 Impact Factor

Cytogenetic heterogeneity in a second primary radiation-induced bladder carcinoma: ten karyotypically unrelated clones.

Cancer Genet Cytogenet 1998 Sep;105(2):134-7

Department of Clinical Genetics, University Hospital, Lund, Sweden.

View Article
September 1998
8 Reads
1 Citation

Chromosome abnormalities in squamous cell carcinoma of the urethra.

Genes Chromosomes Cancer 1998 Sep;23(1):72-3

Department of Clinical Genetics, University Hospital, Lund, Sweden.

View Article
September 1998
7 Reads
4.040 Impact Factor

Chromosomal abnormalities in two bladder carcinomas with secondary squamous cell differentiation.

Cancer Genet Cytogenet 1998 Apr;102(2):125-30

Department of Clinical Genetics, University Hospital, Lund, Sweden.

View Article
April 1998
6 Reads
3 Citations

Chromosomal abnormalities in two bladder carcinomas with secondary squamous cell differentiation

Cancer Genet Cytogenet

Cancer Genet Cytogenetics

Two secondary squamous cell carcinomas of the bladder (i.e., tumors that originated from primary transitional cell carcinomas) were examined cytogenetically. Both tumors showed complex karyotypes with many of the same aberrations that have formerly been described in transitional cell carcinomas. Monosomy 9, trisomy 7, and rearrangements of chromosomes 3, 8, 10, 13, and 17 were common to both tumors. Among other changes that have been implicated in bladder carcinogenesis, an isochromosome for 5p was seen in one tumor and loss of 11p material in the other. Our findings indicate that secondary squamous cell carcinomas of the bladder are karyotypically indistinguishable from advanced transitional cell carcinomas of the same organ. The putative genetic changes that steer the differentiation of the neoplastic epithelium in the direction of squamous cells thus remain unknown.

View Article
April 1998
6 Reads

Clinical, Cytogenetic, and Molecular characterization of Disorders of Sexual Development (DSD) in Sudan

Genomics and Applied Biology

Abstract Sex determination is defined as a genetic events that lead to male or female gonadal-development, whereas sexual differentiation is the subsequent steps leading to functional sexuality and secondary sexual characteristics. DSD have an estimated incidence of 0.1 to 0.5% of live births. It presents in several forms e.g., male and female pseudohermaphroditism, gonadaldysgenesis, and true hermaphroditism. Aims The present study aimed to explore and characterize cytogenetics, molecular alterations, hormonal profile, and clinical aspects of different types of DSD in Sudan for the first time. Methods The study comprised 70 patients, of which 18 raised as males, and 52 as females. Medical history, physical examination, culturing of lymphocytes, chromosomal analysis, PCR analysis for SRY gene, hormonal profile, pelvic ultrasound, laparoscopic examination (when required), histopathology, and psychological assessment were done. Results The vast majority of patients (40%) were adults ≥17-year-old, followed by patients ≤ 2 year-old (33%). Although, 50% of the patients were referred due to genital ambiguity, other cause including primary amenorrhea, failure to thrive, and short stature were reported. Of the 70 patients only 13 patients (19%) were satisfied with their sex of rearing, 5 patients (6%) were not satisfied, 16 patients (23%) could not decide, and 36 patients (51%) were children. Female genital mutilation was performed in almost all adult patients raised as female including those diagnosed as Male pseudohermaphrodites. The analysis showed 23 patients were diagnosed as male pseudohermaphrodit es, 2 as test icular feminizati on syndrome, 14 as complete gonadal dysgenesi s, 8 as fem ale pseudohermaphrodites, 6 as true hermaphrodites, 1 as Smith-Lemli-Opitz syndrome, one case of Down syndrome, and 15 patients were under controversial till the end of the study. Conclusion Based on the study conclusion one may concludes that multidisciplinary approach remains the best strategy for diagnosis and management of such cases. Labor-attending staff should be well trained to avoid sex assignment errors`. Female genital mutation, if performed in DSD, ends the chance of prepuberty diagnosis and complicates the sex-reassignment constructive surgery. Keywords Disorders of sexual development; intersex

View Article
4 Reads

USING OF HIBISCUS SABDARIFFA IN STAINING OF APPENDICULAR TISSUE SECTIONS

European Journal of Biomedical AND Pharmaceutical sciences

Objectives: this was a retrospective descriptive study performed on formalin-fixed paraffin-embedded tissue sections prepared from healthy and/or inflamed Appendix and aimed to assess the quality of staining by Roselle (Hibiscus Sabdariffa) natural extract compared to the routine H&E staining method. Methods: the appendicular tissue sections were grouped and stained by different concentrations of Hibiscus Sabdariffa solution with parallel H&E staining as control. Results: about 20% of sections showed first degree staining quality in 5% solution, more than half of them showed the best staining quality within 60 minutes time duration. Conclusions: Hibiscus Sabdariffa solution can be used effectively in staining of appendicular tissues instead of H&E staining method.

View Article
9 Reads

Interleukin-4 Intron 3 VNTR Polymorphism Gene in Leukemic Patients

Ahmed et al., J Blood Disord Transfus 2016, 7:3

Blood Disorders & Transfusion

Background: Leukemia is a group of chronic malignant disorders of White blood cells and its precursor. Interleukin-4 (IL-4) is inflammatory cytokine that determines the activation and differentiation of B-cells, mast cells, erythroid progenitors. Several studies have investigated the association between IL-4 intron 3 variable number of tandem repeats (VNTR) polymorphism and cancer risk in humans; however, this association is not investigatedamong patients with leukemia. Material and Methods: The present study aimed to investigate genotype and allele frequencies of IL-4 gene intron 3(VNTR) polymorphism in patients with leukemia compared to healthy control. The study included 231 patients with leukemia and 163 healthy controls. Genomic DNA was isolated from 3 ml of anticoagulated venous blood samples by modified salting out method. IL-4 intron 3 VNTR polymorphism determined by using polymerase chain reaction (PCR) with specific primers. The data were analysed using SPSS software program version 21. P value, Odds ratio (OR) and corresponding 95% confidence interval (CI) were used to estimate the strength of the association. Results: The allele frequency was showed in 25.9% (60/231) leukemic patients while 74.1% (171/231) showed absence of allele compared with the presence of allele in all control group with significance differences of P value=0.00 and risk factor of 4.617 times for leukemia. The frequencies of P1P1, P2P2, and P1P2 genotypes of intron 3 VNTR polymorphism in leukemic patients were significantly different from control group P value=0.00.The result showed, P1P1 and P1P2 allele were highly risk for developing leukemia than P2P2 (OR:P1P1 1.24, 95% CI: 0.675-2.279; OR P1P2: 1.24, 95% CI: 0.568-2.7; OR P2P2:0.72, 95% CI: 0.398-1.3). Conclusion: IL-4 intron 3 VNTR polymorphism could influences in the risk of leukemia; this could be used as early prognostic marker in the course of the disease.

View Article
10 Reads

MOSQUITO BORNE PARASITIC DISEASES IN MEROWE DAM AREA- NORTHERN SUDAN

Journal of Science

Merowe dam is located in the Northern State of Sudan across the River Nile. The dam is a hydroelectric and irrigation scheme that may probably develop climatic and environmental changes which might have profound impact on the vectors and vector borne diseases in the area not only by the echo changes but also through the new labors and settlers. The study aimed also to study the distribution of each Plasmodium species in the population of the area and to investigate the possibility of the presence of Wucheraria bancrofti prevalence in the mosquitoes and population as well. A total of 260 volunteers (64.2% of them were females; age ranged between 10 and 92 years) were selected by systematic random sampling method. Demographic and socioeconomic data were obtained using predesigned structured questionnaire. Blood samples were taken on filter papers for screening of Plasmodium spp and Wucheraria bancrofti DNA using real time PCR. The analysis of the genomic DNAs obtained from 260 healthy volunteers, were positive for Plasmodium spp in 135 cases (51.9%). Of these, P. falciparum spp was seen in 121 (89.9%), while P. vivax in only 2 cases (1.5%), and mixed infection was seen in 12cases (8.9%).The PCR analysis showed positive Wucheraria bancrofti in 3 cases out of 260. Plasmodium falciparum is the predominant species detected in the study population, P.vivax has been identified for the first time including mixed infection with P. falciparum in 8.9% of the study population. Wucheraria bancrofti has been detected for the first time in both human subjects and Culex mosquito.

View Article
4 Reads

Improvement of Outcome of Chromosomal Breakage Test Using Novel Statistical Equation

ajmms.20170703.06

American Journal of Medicine and Medical Sciences

This study aimed to develop a statistical equation to determine the amount of blood needed for cytogenetic culture in chromosomal breakage test, based on the white blood cell (WBC) count of the tested patients. Material and Methods: A total of 69 patients with aplastic anemia and a provisional diagnosis of Fanconi anemia were enrolled in the present study. The WBC count and chromosomal breakage test were performed for all patients. Correlation between WBC count and the successful cell cultures was determined using ANOVA test. Then the correlations between WBC count, cell culture result, and quality of metaphases were determined using Pearson's correlation test. The equation was then used to determine the needed amount of blood. Result: Of the 69 cultures, 60(87%) showed an adequate number of metaphase, of which, 8 had excellent metaphase appearance, 25 had good appearance and 27 had a bad appearance. The remaining 9(13%) failed to grow in culture. ANOVA test showed that cell culture success was correlated positively with WBC count (P.value = 0.01), furthermore, Pearson's correlation showed the cell culture result and metaphase quality was correlated positively with WBC count (P.value = 0.00). The equation was established statistically. Conclusion: The equation obtained by the study may improve the success and quality of chromosomal breakage test. However, future study needs to validate the value of the equation.

View Article
3 Reads

Rare Suprasellar Chordoid Meningioma with INI1 gene mutation

sjms.v12i3.1001

Sudan Journal of Medical Sciences

Background: Chordoid Meningioma is a rare brain tumour characterized genetically by loss of genetic material from chromosome 22q at cytogenetic level resulting in mutation of NF2 gene. Objectives and case report: In the present report, we described a rare case of suprasellar chordoid meningioma, which presented in a 32-year-old-woman. Her only complain was throbbing headache. Neurological examination showed left temporal hemianopia, decreased visual acuity (3/6), and no physical abnormalities related to Castleman syndrome were noted. Cranial magnetic resonance (MR) images demonstrated a 28x15 mm mass in the sellar region, which showed iso-to low intensity that enhanced vividly after gadolinium with upwards displacement of the Optic chiasm. Total surgical excision of the tumour was performed and subsequent histological examination of the tumour showed typical histology pattern of chordoid meningioma grade II according to the WHO classification system of meningiomas. Genomic DNA was extracted and mutation analysis for INI1 gene using primer of exon 4, 5, 7, and 9 showed mutation involving exon 9. DNA sequencing showed heterozygosity C-T mutation in exon 9 of INI1 gene leading to change of amino acid serine to phenylalanine at (codon 63). The details of this case are presented with a review of the literature.

View Article
7 Reads