Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing China
Chaoyang, Beijing | China
Specialties: Molecular Developmental Biology
Iftikhar Ali is a Pakistani born male young PhD scholar in the institute of genetics and
developmental Biology, Chinese Academy of Sciences, Beijing, China. He started his early
education in Pakistan and completed his Master of Philosophy degree in the subject of Plant
Biochemistry and molecular Biology from the top ranked university of Pakistan, Quaid-i-Azam
University, Islamabad. He published five research articles in the prestigious journals with high
impact points during his MPhil research work. He worked on molecular aspects of different
plants from the simplest one like Arabidopsis to the most complex like wheat and rice. He also
served on the post of executive in research department Axproz Islamabad. He knows three
languages as natives (Urdu, Pashto and Punjabi) and English and Chinese as a professional language. He
obtained a certificate of good performance for Lab practicals to M. Sc Students during his
professional experience in the lab of plant Biochemistry and Molecular Biology. Biochemistry,
molecular biology, epigenetics and molecular and structural genetics are the research fields of his
most interest. The success of his MPhil research was that he reported three new alleles which
were previously unknown for researchers and the work was published in prestigious journal of
Theoretical Biology under the title “Molecular characterization of the Puroindoline-a and b
alleles in synthetic hexaploid wheats and in silico functional and structural insights into PinaD1”.
More recently, he started his PhD research work in the key state laboratory of molecular
Biology under the supervision of Prof. Weicia Yang, Director IGDB, CAS, and developed at
least one hundred new Arabidopsis pollen mutants in a short period of eight months. The main
focus of his PhD research is to determine functional insights of genes involved in male
Supervisors: Iftikhar Ali and Prof. Dr. Yang Weicai
Address: Institute of Genetics and Developmental Biology, Chinese Academy of Sciences,
No.1 West Beichen Road, Chaoyang District, Beijing, 100101 P.R. China
Primary Affiliation: Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing China - Chaoyang, Beijing , China
Journal of Theoretical Biology
Kernel hardness determined by two tightly linked Puroindoline genes, Pina-D1 and Pinb-D1, located on chromosome 5DS define commercially important characteristics, uses, major grades and export markets of wheat. This study was conducted to characterize Pina-D1 and Pinb-D1 alleles, in fifteen synthetic hexaploid wheats (SHWs) and its relation with grain hardness. Additionally, in silico functional analyses of puroindoline-a protein was conducted for better understanding of their putative importance in grain quality. Six different Pina-D1 alleles were identified in the SHWs, of which three i.e. Pina-D1a, Pina-D1c and Pina-D1d were already known whereas the other three had new sequence polymorphisms and were designated as Pina-D1w, Pina-D1x and Pina-D1y. Three different Pinb-D1 alleles were identified which have been reported earlier and no novel sequence polymorphism was detected. It was concluded that despite some primary, secondary and 3D structure variations, ligand binding sites and disulfide bonds discrepancies, the main features of PINA, i.e. the tryptophan-rich domain, the cysteine backbone, the signal peptide and basic identity of the proteins were all conserved. In silico analysis showed that puroindolines having binding capacity with small parts of prolamins causing celiac disease of human, however their potential role is not obvious. Conclusively, the new Pina-D1 alleles with modest effect on grain hardness, and insight into their functional and structural characteristics are important findings and their putative role in celiac disease require further studies to validate.
Front Genet. 2017 Jul 19;8:100. doi: 10.3389/fgene.2017.00100. eCollection 2017
Frontiers in Genetics
Dynamic DNA modifications, such as methylation/demethylation on cytosine, are major epigenetic mechanisms to modulate gene expression in both eukaryotes and prokaryotes. In addition to the common methylation on the 5th position of the pyrimidine ring of cytosine (5mC), other types of modifications at the same position, such as 5-hydroxymethyl (5hmC), 5-formyl (5fC), and 5-carboxyl (5caC), are also important. Recently, 5hmC, a product of 5mC demethylation by the Ten-Eleven Translocation family proteins, was shown to regulate many cellular and developmental processes, including the pluripotency of embryonic stem cells, neuron development, and tumorigenesis in mammals. Here, we review recent advances on the generation, distribution, and function of 5hmC modification in mammals and discuss its potential roles in plants.
Mater Sci Eng C Mater Biol Appl 2017 Apr 28;73:665-669. Epub 2016 Dec 28.
Department of physics, College of Science, Al Imam Mohammad Ibn Saud Islamic University (IMSIU), Riyadh 11623, Saudi Arabia.
Turk J Bot(2015) 39: © TÜBİTAKdoi:10.3906/bot-1405-48
Turkish Journal of Botany
Germins and germin-like proteins constitute a ubiquitous family of plant proteins and have a role in the defense against pathogen attacks. In the present work the Oryza sativa germin-like protein 1 (OsGLP1) and putative germin A (OsGerA) promoter regions of five Pakistani rice varieties were analyzed for variations in expression regulation of these promoters and similarity with others germin and germin-like protein promoters. Phylogenetic analyses showed that the studied OsGLP1 promoters were tightly clustered around the ancestral group while putative OsGerA clustered around the descendant group. HADDOCK was used for docking selected transcription factors (TFs) with its corresponding regulatory elements, for determining the most stable interaction with the highest probability of hydrogen bonding between them. TATA box binding protein (TBP) elements mainly existed on the 3′ regions very near the gene, while far regions of promoters mostly lacked such elements. This study showed that TBP not only binds to (-30) TATA box element, but also binds to a wide range of elements at different rates, positioned on about a thousand base pairs upstream region of germin and germin-like genes. Arginine was found to be the most reactive residue in TFs, while adenine was the most sensitive in regulatory elements.