Publications by authors named "Iaroslav Kolesnikov"

3 Publications

  • Page 1 of 1

Tumor Cell-Specific 2'-Fluoro RNA Aptamer Conjugated with -Dodecaborate as A Potential Agent for Boron Neutron Capture Therapy.

Int J Mol Sci 2021 Jul 7;22(14). Epub 2021 Jul 7.

Institute of Chemical Biology and Fundamental Medicine, Siberian Division of Russian Academy of Sciences, 630090 Novosibirsk, Russia.

Boron neutron capture therapy (BNCT) is a binary radiotherapeutic approach to the treatment of malignant tumors, especially glioblastoma, the most frequent and incurable brain tumor. For successful BNCT, a boron-containing therapeutic agent should provide selective and effective accumulation of B isotope inside target cells, which are then destroyed after neutron irradiation. Nucleic acid aptamers look like very prospective candidates for carrying B to the tumor cells. This study represents the first example of using 2'-F-RNA aptamer GL44 specific to the human glioblastoma U-87 MG cells as a boron delivery agent for BNCT. The -dodecaborate residue was attached to the 5'-end of the aptamer, which was also labeled by the fluorophore at the 3'-end. The resulting bifunctional conjugate showed effective and specific internalization into U-87 MG cells and low toxicity. After incubation with the conjugate, the cells were irradiated by epithermal neutrons on the Budker Institute of Nuclear Physics neutron source. Evaluation of the cell proliferation by real-time cell monitoring and the clonogenic test revealed that boron-loaded aptamer decreased specifically the viability of U-87 MG cells to the extent comparable to that of B-boronophenylalanine taken as a control. Therefore, we have demonstrated a proof of principle of employing aptamers for targeted delivery of boron-10 isotope in BNCT. Considering their specificity, ease of synthesis, and large toolkit of chemical approaches for high boron-loading, aptamers provide a promising basis for engineering novel BNCT agents.
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http://dx.doi.org/10.3390/ijms22147326DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8307642PMC
July 2021

Measurement of the Li(p,p'γ)Li reaction cross-section and 478 keV photon yield from a thick lithium target at proton energies from 0.7 to 1.85 MeV.

Appl Radiat Isot 2021 Sep 9;175:109821. Epub 2021 Jun 9.

Budker Institute of Nuclear Physics, Novosibirsk, Russia; Novosibirsk State University, Novosibirsk, Russia. Electronic address:

The Li (p,p'γ)Li reaction cross section and photon yield from a thick lithium target at proton energies from 0.7 to 1.85 MeV have been measured with a HPGe gamma-ray spectrometer. The spectrometer is calibrated on total and relative sensitivity by reference radionuclide sources of photon radiation. The measurement results are compared with those presented in the EXFOR nuclear reaction database and with other data published in open sources. The reliability of the results of previous studies is analyzed.
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http://dx.doi.org/10.1016/j.apradiso.2021.109821DOI Listing
September 2021

Neutron Source Based on Vacuum Insulated Tandem Accelerator and Lithium Target.

Biology (Basel) 2021 Apr 21;10(5). Epub 2021 Apr 21.

Faculty of Physics, Novosibirsk State University, 2 Pirogov Str., 630090 Novosibirsk, Russia.

A compact accelerator-based neutron source has been proposed and created at the Budker Institute of Nuclear Physics in Novosibirsk, Russia. An original design tandem accelerator is used to provide a proton beam. The proton beam energy can be varied within a range of 0.6-2.3 MeV, keeping a high-energy stability of 0.1%. The beam current can also be varied in a wide range (from 0.3 mA to 10 mA) with high current stability (0.4%). In the device, neutron flux is generated as a result of the Li(p,n)Be threshold reaction. A beam-shaping assembly is applied to convert this flux into a beam of epithermal neutrons with characteristics suitable for BNCT. A lot of scientific research has been carried out at the facility, including the study of blistering and its effect on the neutron yield. The BNCT technique is being tested in in vitro and in vivo studies, and the methods of dosimetry are being developed. It is planned to certify the neutron source next year and conduct clinical trials on it. The neutron source served as a prototype for a facility created for a clinic in Xiamen (China).
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http://dx.doi.org/10.3390/biology10050350DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8143170PMC
April 2021
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