Publications by authors named "Hyun-Woo Kim"

474 Publications

EcoBank: A flexible database platform for sharing ecological data.

Biodivers Data J 2021 17;9:e61866. Epub 2021 Feb 17.

National Institute of Ecology, Seocheon-gun, South Korea National Institute of Ecology Seocheon-gun South Korea.

Background: Environmental crisis challenges the human race harder than ever before. Ecologists have produced a massive amount of data to cope with the crisis. Accordingly, many national scale ecological database systems have been developed worldwide to manage and analyse these datasets. However, in Korea, ecological datasets produced by different research institutes for different purposes have not been integrated or serviced due to poorly designed information infrastructure. To address this obstacle, we present EcoBank (www.nie-ecobank.kr), an open, web-based ecological database platform designed to play an important role in ecosystem analysis, not only in Korea, but also worldwide.

New Information: The architecture of EcoBank comprises core technologies of WebGIS, Application Programming Interface (API), responsive web and open-source software (OSS). Comprehensive ecological datasets from three different sources, including the National Institute of Ecology (NIE) in Korea, related national and international platforms and repositories, enter the three conceptual modules in EcoBank: data management, analysis and service. Diverse potential stakeholders of EcoBank can be classified into three groups: researchers, policy-makers and public users. EcoBank aims to expand its horizons through mutual communication amongst these stakeholders. We opened and launched the EcoBank service in December 2019 and have now begun to broaden its network by linking it to other data platforms and repositories over the globe to find possible solutions to ecological issues in Korea.
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http://dx.doi.org/10.3897/BDJ.9.e61866DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7904746PMC
February 2021

Complete mitochondrial genome of Kishi velvet shrimp, (Rathbun, 1902) (Crustacea: Decapoda: Penaeidae) in the East China Sea.

Mitochondrial DNA B Resour 2016 Mar 28;1(1):120-121. Epub 2016 Mar 28.

Interdisciplinary Program of Biomedical Engineering, Pukyong National University, Busan, Republic of Korea.

We determined the complete mitochondrial genome of (Rathbun, 1902, which is collected from East China Sea (124°E and 33.5°N). Total mitochondrial genome length of was 15 939 bp, in which 13 proteins, two ribosomal RNAs, 22 transfer RNAs and a putative control region were encoded. The gene arrangement of was well conserved with nine known Penaeidae mitochondrial genomes from to . The protein-coding genes started with ATN except for in which ACG is used. Four genes ( and ) exhibited an incomplete stop codon. Nucleotide sequence identity of mitochondrial genome to those of nine Penaeidae species ranged from 78% to 80%. Based on the COI region, is most closely related to .
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http://dx.doi.org/10.1080/23802359.2016.1143341DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7871848PMC
March 2016

Teaching Video NeuroImages: Periodic Alternating Nystagmus in Paraneoplastic KLHL11 Rhomboencephalitis.

Neurology 2021 Feb 15. Epub 2021 Feb 15.

From the Department of Neurology (P.B., H.W.K., A.S.L.C.), Mayo Clinic; Departments of Neurology and Ophthalmology (E.R.E.), Mayo Clinic, Jacksonville, FL; Department of Neurology (D.D.), Mayo Clinic, Rochester, MN.

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http://dx.doi.org/10.1212/WNL.0000000000011711DOI Listing
February 2021

Sesquiterpenoids from the Aerial Parts of with Inhibitory Activities on Proprotein Convertase Subtilisin/Kexin Type 9 Expression.

J Nat Prod 2021 Feb 11;84(2):220-229. Epub 2021 Feb 11.

College of Pharmacy and Research Institute of Pharmaceutical Sciences, Seoul National University, Seoul 08826, Republic of Korea.

Phytochemical investigation of the methanol extract of the aerial parts of aided by a proprotein convertase subtilisin/kexin type 9 (PCSK9) mRNA expression screening assay in HepG2 cells led to the identification of 19 compounds including one new norsesquiterpene (), six new eudesmane sesquiterpenoids (-, , and ), and 12 known compounds. The structures of all compounds were elucidated by interpretation of their 1D and 2D NMR spectroscopic and MS data. Furthermore, computational prediction of ECD or chemical shifts was used to propose the absolute configurations of the new structures. All isolates were assessed for their inhibitory activities against PCSK9 mRNA expression and PCSK9-low-density lipoprotein receptor (LDLR) interactions. None of the isolated compounds inhibited PCSK9 and LDLR interactions. However, compounds , , and downregulated PCSK9 mRNA expression.
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http://dx.doi.org/10.1021/acs.jnatprod.0c00829DOI Listing
February 2021

Bee venom reduces burn-induced pain via the suppression of peripheral and central substance P expression in mice.

J Vet Sci 2021 Jan;22(1):e9

Department of Physiology and Medical Science, College of Medicine and Brain Research Institute, Chungnam National University, Daejeon 35015, Korea.

Background: Scalding burn injuries can occur in everyday life but occur more frequently in young children. Therefore, it is important to develop more effective burn treatments.

Objectives: This study examined the effects of bee venom (BV) stimulation on scalding burn injury-induced nociception in mice as a new treatment for burn pain.

Methods: To develop a burn injury model, the right hind paw was immersed temporarily in hot water (65°C, 3 seconds). Immediately after the burn, BV (0.01, 0.02, or 0.1 mg/kg) was injected subcutaneously into the ipsilateral knee area once daily for 14 days. A von Frey test was performed to assess the nociceptive response, and the altered walking parameters were evaluated using an automated gait analysis system. In addition, the peripheral and central expression changes in substance P (Sub P) were measured in the dorsal root ganglion and spinal cord by immunofluorescence.

Results: Repeated BV treatment at the 2 higher doses used in this study (0.02 and 0.1 mg/kg) alleviated the pain responses remarkably and recovered the gait performances to the level of acetaminophen (200 mg/kg, intraperitoneal, once daily), which used as the positive control group. Moreover, BV stimulation had an inhibitory effect on the increased expression of Sub P in the peripheral and central nervous systems by a burn injury.

Conclusions: These results suggest that a peripheral BV treatment may have positive potency in treating burn-induced pain.
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http://dx.doi.org/10.4142/jvs.2021.22.e9DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7850793PMC
January 2021

Clinical significance of Epstein-Barr virus in the cerebrospinal fluid of immunocompetent patients.

Clin Neurol Neurosurg 2021 Jan 19;202:106507. Epub 2021 Jan 19.

Department of Neurology, Biomedical Research Institute, Pusan National University Hospital, Busan, Republic of Korea; Department of Neurology, Pusan National University Yangsan Hospital, Pusan National University, Yangsan, Republic of Korea.

Introduction: Polymerase chain reaction (PCR)-based testing of cerebrospinal fluid (CSF) samples has greatly facilitated the diagnosis of central nervous system (CNS) infections. However, the clinical significance of Epstein-Barr virus (EBV) DNA in CSF of individuals with suspected CNS infection remains unclear. We wanted to gain a better understanding of EBV as an infectious agent in immunocompetent patients with CNS disorders.

Methods: We identified cases of EBV-associated CNS infections and reviewed their clinical and laboratory characteristics. The study population was drawn from patients with EBV PCR positivity in CSF who visited Pusan National University Hospital between 2010 and 2019.

Results: Of the 780 CSF samples examined during the 10-year study period, 42 (5.4 %) were positive for EBV DNA; 9 of the patients (21.4 %) were diagnosed with non-CNS infectious diseases, such as optic neuritis, Guillain-Barré syndrome, and idiopathic intracranial hypotension, and the other 33 cases were classified as CNS infections (22 as encephalitis and 11 as meningitis). Intensive care unit admission (13/33 patients, 39.3 %) and presence of severe neurological sequelae at discharge (8/33 patients, 24.2 %) were relatively frequent. In 10 patients (30.3 %), the following pathogens were detected in CSF in addition to EBV: varicella-zoster virus (n = 3), cytomegalovirus (n = 2), herpes simplex virus 1 (n = 1), herpes simplex virus 2 (n = 1), Streptococcus pneumomiae (n = 2), and Enterococcus faecalis (n = 1). The EBV-only group (n = 23) and the co-infection group (n = 10) did not differ in age, gender, laboratory data, results of brain imaging studies, clinical manifestations, or prognosis; however, the co-infected patients had higher CSF protein levels.

Conclusion: EBV DNA in CSF is occasionally found in the immunocompetent population; the virus was commonly associated with encephalitis and poor prognosis, and frequently found together with other microbes in CSF.
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http://dx.doi.org/10.1016/j.clineuro.2021.106507DOI Listing
January 2021

A large palmar lipoma arising from flexor tenosynovium of the hand causing digital nerve compression: A case report.

Jt Dis Relat Surg 2021 6;32(1):230-233. Epub 2021 Jan 6.

Nanoori 2bko R&D Institute, Nanoori Hospital Suwon, 295, Jungbu-daero, Yeongtong-gu, Gyeonggi-Do, Republic of Korea.

Lipoma is a common benign soft tissue tumor which rarely occurs in the hand. Lipomas of the hand seldomly cause pain or other symptoms. However, in certain areas, the mass effect from the lipoma may cause clinical symptoms. It rarely involves the deep areas such as synovial membrane and muscle and, sometimes, pressure on the peripheral nerves can cause pain and neurological symptoms. The treatment is surgical resection with a low rate of recurrence. However, the rate of recurrence is high in deep and infiltrating lipomas which preclude complete resection. In this article, we report a case of a large palmar lipoma arising from the flexor tenosynovium of the hand causing digital nerve compression.
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http://dx.doi.org/10.5606/ehc.2021.75678DOI Listing
January 2021

Characterization of the complete mitochondrial genome of brown barracuda, (Perciformes: Sphyraenidae).

Mitochondrial DNA B Resour 2020 Jul 28;5(3):3042-3043. Epub 2020 Jul 28.

Industrial Convergence Bionix Engineering, Pukyong National University, Busan, Republic of Korea.

The entire mitochondrial genome sequence of collected from Korean water was determined by the Next Generation Sequencing (NGS) technology. Its total length was 16,620 bps in length, which possessed the canonical 37 genes in the eukaryotes. Unusual start codon was exclusively found in COX1(GTG), while incomplete stop codons (TA-/T-) were identified in ATP6, COX2, ND3, ND4, and Cyt b. A phylogenetic analysis with currently identified full mitogenomes in Perciformes, was most closely related to S. (76.87%) and S. (76.84%). This mitogenome sequence would explain the evolution of genus .
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http://dx.doi.org/10.1080/23802359.2020.1797588DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7782257PMC
July 2020

Comparison of human poly-N-acetyl-lactosamine synthase structure with GT-A fold glycosyltransferases supports a modular assembly of catalytic subsites.

J Biol Chem 2020 Dec 3:100110. Epub 2020 Dec 3.

Department of Biochemistry & Molecular Biology, University of Georgia, Athens, GA 30602, USA. Electronic address:

Poly-N-acetyl-lactosamine (poly-LacNAc) structures are composed of repeating [-Galβ(1,4)-GlcNAcβ(1,3)-] glycan extensions. They are found on both N- and O-glycoproteins and glycolipids, and play an important role in development, immune function, and human disease. The majority of mammalian poly-LacNAc is synthesized by the alternating iterative action of β1,3-N-acetylglucosaminyltransferase 2 (B3GNT2) and β1,4-galactosyltransferases. B3GNT2 is in the largest mammalian glycosyltransferase family, GT31, but little is known about the structure, substrate recognition, or catalysis by family members. Here we report the structures of human B3GNT2 in complex with UDP:Mg, and in complex with both UDP:Mg and a glycan acceptor, lacto-N-neotetraose. The B3GNT2 structure conserves the GT-A fold and the DxD motif that coordinates a Mg ion for binding the UDP-GlcNAc sugar donor. The acceptor complex shows interactions with only the terminal Galβ(1,4)-GlcNAcβ(1,3)- disaccharide unit, which likely explains the specificity for both N- and O-glycan acceptors. Modeling of the UDP-GlcNAc donor supports a direct displacement inverting catalytic mechanism. Comparative structural analysis indicates that nucleotide sugar donors for GT-A fold glycosyltransferases bind in similar positions and conformations without conserving interacting residues, even for enzymes that use the same donor substrate. In contrast, the B3GNT2 acceptor binding site is consistent with prior models suggesting that the evolution of acceptor specificity involves loops inserted into the stable GT-A fold. These observations support the hypothesis that GT-A fold glycosyltransferases employ co-evolving donor, acceptor, and catalytic subsite modules as templates to achieve the complex diversity of glycan linkages in biological systems.
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http://dx.doi.org/10.1074/jbc.RA120.015305DOI Listing
December 2020

Mitogenome Announcement Characterization of the complete mitochondrial genome of golden tank goby, (Perciformes: Gobiidae).

Mitochondrial DNA B Resour 2020 Dec 24;5(4):3817-3818. Epub 2020 Dec 24.

Industrial Convergence Bionix Engineering, Pukyong National University, Busan, Republic of Korea.

We applied next-generation sequencing (NGS) method to construct the complete mitochondrial genome of . The obtained mitogenome of (16,590 bp) exhibited a typical structure harboring 13 protein-coding genes (PCGs), 22 transfer RNAs (tRNAs), two ribosomal RNAs (rRNAs), and one control regions (D-loop). Most of mitochondrial genes are encoded on the heavy (H) strand, except for eight tRNAs and ND6. Unusual start codons were identified in COX1 (GTG) and ATP6 (TTG). Six genes (ND2, COX2, COX3, ND3, ND4, and CytB) were terminated by an incomplete stop codon (TA-/T-). A phylogenetic study showed that formed a clade distinct from other species in the subfamily Gobiinae. was most closely related to with 87.04% sequence identity among the four species in the genus .
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http://dx.doi.org/10.1080/23802359.2020.1840943DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7759244PMC
December 2020

Characterization of the complete mitochondrial genome of Golden cusk, (Ophidiiformes:Ophidiidae).

Mitochondrial DNA B Resour 2020 Dec 24;5(4):3815-3816. Epub 2020 Dec 24.

Department of Marine Biology, Pukyong National University, Busan, Republic of Korea.

The complete mitochondrial genome of was determined by the bioinformatic assembly of the next generation sequencing (NGS) reads. Total length of the mitogenome was 16,717 bp, which harbors the conserved 13 protein-coding genes (PCGs), 2 ribosomal RNAs (12S and 16S), 22 tRNAs, and two non-coding region (the control region and the origin of light-strand replication). Among 13 protein-coding genes, unusual start codon (GTG) was exclusively identified in COX1, while the incomplete stop codons (TA- or T-) were detected in COX2, COX3, ND2, ND3, ND4 and CytB. As a result of phylogenetic tree, formed a cluster of the family Ophidiidae together with and .
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http://dx.doi.org/10.1080/23802359.2020.1840942DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7759297PMC
December 2020

Phylogenetic Positioning of a Strongyloides stercoralis Isolate Recovered from a Korean Patient and Comparison with Other Asian Isolates.

Korean J Parasitol 2020 Dec 29;58(6):689-694. Epub 2020 Dec 29.

Department of Parasitology and Tropical Medicine, School of Medicine, Pusan National University, Yangsan 50612, Korea.

Strongyloidiasis is caused by Strongyloides stercoralis and is one of the most neglected tropical diseases in tropical and subtropical regions. Although several strongyloidiasis cases have been reported in Korea, genetic analysis of Korean isolates is still incomplete. In this study, a parasite was isolated from a 61-year-old man diagnosed with strongyloidiasis during the treatment of lymphoma on his retroperitoneal lymph node. Diffuse symmetric wall thickening from the ascending to descending colon and a nematode-infected intestine was observed following microscopic examination. Genomic DNA was isolated from a patient tissue block, and S. stercoralis was identified by PCR and sequencing (18S rDNA). In order to determine phylogenetic location of a Korean isolate (named KS1), we analyzed cox1 gene (500-bp) and compared it with that from 47 previous S. stercoralis isolates (28 human isolates and 19 canid isolates) from Asian countries. Our results showed that phylogenetic tree could clearly be divided into 5 different groups according to hosts and regions. KS1 was most closely related with the Chinese isolates in terms of genetic distance.
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http://dx.doi.org/10.3347/kjp.2020.58.6.689DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7806424PMC
December 2020

Migrations of young spotted seals (Phoca largha) from Peter the Great Bay, Sea of Japan/East Sea, and the pattern of their use of seasonal habitats.

PLoS One 2021 5;16(1):e0244232. Epub 2021 Jan 5.

National Institute of Fisheries Science, Cetacean Research Institute, Ulsan, Republic of Korea.

We studied the migrations of young spotted seals during their annual cycle. In May 2017, we attached satellite tags (SPOT-293A) to three individuals (two underyearlings and one yearling) captured at their breeding ground in Peter the Great Bay, western Sea of Japan/East Sea. The operational time of the installed tags ranged from 207 to 333 days; a total of 27195 locations were uploaded. All three seals migrated east and further north along the coast of the mainland. The average daily migration speed of the seals ranged between 70 and 135 km/day. The yearling moved faster than the underyearlings. During early August, they arrived at their summer habitats, which were located in the northern part of the Tatar Strait (Sea of Japan/East Sea) for the underyearling seals and in Aniva Bay (Sea of Okhotsk) for the yearling seal. While moving from the place of tagging to the summer feeding grounds, the seals covered a distance of 2300 to 3100 km. From August to October, each seal permanently stayed within the same isolated area. The reverse migration of all three seals began in November. When the seals traveled south, they used the same routes by which they had moved north in the spring, but they moved at a faster speed. By December, two seals returned to their natal islands, where both stayed until their transmitters stopped sending signals (in March 2018).
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http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0244232PLOS
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7785119PMC
January 2021

The complete mitochondrial genome of the doubled-lined mackerel Rüppell, 1836 (Perciformes: Scombridae) from Beqa lagoon in Fiji.

Mitochondrial DNA B Resour 2020 Nov 6;5(3):3688-3689. Epub 2020 Nov 6.

Department of 4th Industrial Convergence Bionix Engineering, Pukyong National University, Busan, Republic of Korea.

The complete mitochondrial genome of the doubled-lined mackerel, was determined by the combination of high-throughput sequencing (HTS) and Sanger sequencing. The constructed mitochondrial genome of was 16,537 bp in length, which harbors a canonical 37 genes (13 proteins, two ribosomal RNAs, and 22 tRNAs) and two non-coding regions (origin of light-strand replication (O) and the D-loop control region). Among 38 genes, nine were encoded on its light strand (L), while the other 28 were on its heavy strand (H). Besides COX1 (GTG) and ATP6 (CTG), the other eleven protein-coding genes (PCGs) begin with a typical start codon (ATG). The phylogenetic tree showed that was not clustered with the other species in the Scombridae, forming a clade for . The genetic information of will provide useful information for the scientific management and conservation of the species in the genus.
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http://dx.doi.org/10.1080/23802359.2020.1832936DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7655050PMC
November 2020

Characterization of complete mitochondrial genome of (Perciformes: Artedidraconidae).

Mitochondrial DNA B Resour 2019 Dec 12;5(1):156-157. Epub 2019 Dec 12.

Interdisciplinary Program of Biomedical, Mechanical and Electrical Engineering, Pukyong National University, Busan, Republic of Korea.

The complete mitochondrial genome of was determined by the MiSeq platform, which was the first report in the family Artedidraconidae. The circular form of its mitochondrial genome was 17,086 bp, which contained the canonical eukaryotic 37 genes. The gene orders of was identical to the other icefish species, in which there was additional non-coding region and translocation of ND6 gene. Except for ATP6 gene and COI (GTG), 11 genes begin with the typical start codon, while incomplete stop codons (T- -) were identified in COII, ND4, and CytB. Phylogenetic tree with the currently known mitogenomes in suborder Notothenioidei showed that was located distinctly from those in Bathydraconidae and Nototheniidae forming a unique cluster as Artedidraconidae. The first complete mitochondrial genome of would be the fundamental data to understand the evolutional relationship of icefish species in the Antarctic Oceans.
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http://dx.doi.org/10.1080/23802359.2019.1698361DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7720972PMC
December 2019

Characterization of the complete mitochondrial genome of (Perciformes, Channichthyidae).

Mitochondrial DNA B Resour 2019 Nov 8;4(2):3914-3915. Epub 2019 Nov 8.

Interdisciplinary Program of Biomedical, Mechanical and Electrical Engineering, Pukyong National University, Busan, Republic of Korea.

The complete mitochondrial genome sequence of the Icefish, was determined by the Next Generation Sequencing (NGS) analysis. The complete mitogenome was 17,452 bp in length, which encoded the canonical 13 protein-coding genes, 22 tRNAs, two rRNAs, and two non-coding regions. As shown in the other notothenids, translocation of ND6 and an additional non-coding region were identified, which is different from the typical vertebrate mitochondrial genomes. The was clustered distinctly from the those in the and , which supported the idea that this species should be classified in the different genus, in the family Channichthyidae.
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http://dx.doi.org/10.1080/23802359.2019.1688112DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7707690PMC
November 2019

Characterization of the complete mitochondrial genome of collected from Nakdong River, South Korea.

Mitochondrial DNA B Resour 2019 Nov 8;4(2):3908-3909. Epub 2019 Nov 8.

Interdisciplinary Program of Biomedical, Mechanical and Electrical Engineering, Pukyong National University, Busan, Republic of Korea.

The complete mitochondrial genome of collected from a native Korean river was determined by the bioinformatics assembly of the next-generation sequencing (NGS) reads. The circular mitogenome was 17,590 bp length which harbored canonical 13 protein-coding genes, 22 tRNAs, and 2 rRNAs, which was identical to those of family Odontobutidae. Twenty-eight genes were located on H strand, whereas remaining nine genes were on L strand. Except for COX1 gene (GTG), other 12 protein-coding genes were predicted typical start codons (ATG). Among the currently known mitogenome sequences, showed highest identity (96.98%) to Korean haplotype of (NC010199).
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http://dx.doi.org/10.1080/23802359.2019.1687362DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7707751PMC
November 2019

Characterization of the complete mitochondrial genome of in Korean river.

Mitochondrial DNA B Resour 2019 Nov 8;4(2):3886-3887. Epub 2019 Nov 8.

Interdisciplinary Program of Biomedical, Mechanical and Electrical Engineering, Pukyong National University, Busan, Republic of Korea.

The complete mitochondrial genome of firstly collected from a native Korean river was determined by the bioinformatics assembly of the next-generation sequencing (NGS) reads. The circular mitogenome was 16,525 bp in length which harbored canonical 13 protein-coding genes, 22 tRNAs, and 2 rRNAs, which was identical to those of family Siluridae. Twenty-eight genes were located on H strand, whereas the remaining nine genes were on L strand. Except for COX1 gene (GTG), other 12 protein-coding genes were predicted typical start codons (ATG). Among the currently known mitogenome sequences, S showed highest identity (99.38%) to the Chinese haplotype of (NC022723) followed by the Chinese haplotype of (JX087351). Interestingly, intraspecies variations of are higher than those of interspecies and further study should be made to elucidate the evolutional relationship between two species.
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http://dx.doi.org/10.1080/23802359.2019.1687347DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7707740PMC
November 2019

Percutaneous Trans-splenic Obliteration for Duodenal Variceal bleeding: A Case Report.

Korean J Gastroenterol 2020 12;76(6):331-336

Department of Internal Medicine, Busan Paik Hospital, Inje University College of Medicine, Busan, Kore.

Duodenal varices are a serious complication of portal hypertension. Bleeding from duodenal varices is rare, but when bleeding does occur, it is massive and can be fatal. Unfortunately, the optimal therapeutic modality for duodenal variceal bleeding is unclear. This paper presents a patient with duodenal variceal bleeding that was managed successfully using percutaneous trans-splenic variceal obliteration (PTVO). A 56-year-old man with a history of alcoholic cirrhosis presented with a 6-day history of melena. Emergency esophagogastroduodenoscopy revealed a large, bluish mass with a nipple sign in the second portion of the duodenum. Coil embolization of the duodenal varix was performed via a trans-splenic approach (i.e., PTVO). The patient no longer complained of melena after treatment. The duodenal varix was no longer visible at the follow-up esophagogastroduodenoscopy performed three months after PTVO. The use of PTVO might be a viable option for the treatment of duodenal variceal bleeding.
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http://dx.doi.org/10.4166/kjg.2020.123DOI Listing
December 2020

High Salt Intake Recruits Tonic Activation of NR2D Subunit-Containing Extrasynaptic NMDARs in Vasopressin Neurons.

J Neurosci 2021 Feb 10;41(6):1145-1156. Epub 2020 Dec 10.

Department of Physiology, Chungnam National University, Daejeon 35015, Republic of Korea

In addition to producing a classical excitatory postsynaptic current via activation of synaptic NMDA receptors (NMDARs), glutamate in the brain also induces a tonic NMDAR current () via activation of extrasynaptic NMDARs (eNMDARs). However, since Mg blocks NMDARs in nondepolarized neurons, the potential contribution of eNMDARs to the overall neuronal excitatory/inhibitory (E/I) balance remains unknown. Here, we demonstrate that chronic (7 d) salt loading (SL) recruited NR2D subunit-containing NMDARs to generate an Mg-resistant tonic in nondepolarized [ (holding potential) -70 mV] vasopressin (VP; but not oxytocin) supraoptic nucleus (SON) neurons in male rodents. Conversely, in euhydrated (EU) and 3 d SL mice, Mg-resistant tonic was not observed. Pharmacological and genetic intervention of NR2D subunits blocked the Mg-resistant tonic in VP neurons under SL conditions, while an NR2B antagonist unveiled Mg-sensitive tonic but not Mg-resistant tonic In the EU group VP neurons, an Mg-resistant tonic was not generated by increased ambient glutamate or treatment with coagonists (e.g., d-serine and glycine). Chronic SL significantly increased NR2D expression but not NR2B expression in the SON relative to the EU group or after 3 d under SL conditions. Finally, Mg-resistant tonic selectively upregulated neuronal excitability in VP neurons under SL conditions, independent of ionotropic GABAergic input. Our results indicate that the activation of NR2D-containing NMDARs constitutes a novel mechanism that generates an Mg-resistant tonic in nondepolarized VP neurons, thus causing an E/I balance shift in VP neurons to compensate for the hormonal demands imposed by a chronic osmotic challenge. The hypothalamic supraoptic nucleus (SON) consists of two different types of magnocellular neurosecretory cells (MNCs) that synthesize and release the following two peptide hormones: vasopressin (VP), which is necessary for regulation of fluid homeostasis; and oxytocin (OT), which plays a major role in lactation and parturition. NMDA receptors (NMDARs) play important roles in shaping neuronal firing patterns and hormone release from the SON MNCs in response to various physiological challenges. Our results show that prolonged (7 d) salt loading generated a Mg-resistant tonic NMDA current mediated by NR2D subunit-containing receptors, which efficiently activated nondepolarized VP (but not OT) neurons. Our findings support the hypothesis that NR2D subunit-containing NMDARs play an important adaptive role in adult brain in response to a sustained osmotic challenge.
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http://dx.doi.org/10.1523/JNEUROSCI.1742-20.2020DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7888215PMC
February 2021

Two-oscillator mapping modification of the Poisson bracket mapping equation formulation of the quantum-classical Liouville equation.

J Chem Phys 2020 Dec;153(21):214103

Department of Chemistry, Korea Advanced Institute of Science and Technology (KAIST), Daejeon 34141, South Korea.

Mapping basis solutions provide efficient ways for simulating mixed quantum-classical (MQC) dynamics in complex systems by matching multiple quantum states of interest to some fictitious physical states. Recently, various MQC methods were devised such that two harmonic oscillators are employed to represent each electronic state, showing improvements over one-oscillator-based methods. Here, we introduce and analyze newly modified mapping approximations of the quantum-classical Liouville equation (QCLE) using two oscillators for each electronic state. We design two separate mapping relations that we can adopt toward simulating dynamics and computing expectation values. Through the process, two MQC methods can be constructed, one of which actually reproduces the population dynamics of the forward and backward trajectory solution of QCLE. By applying the methods to spin-boson systems with a range of parameters, we find out that the choice of mapping relations greatly affects the simulation results. We also show that further improvement is possible through using modified identity operator formulations. Our findings may be helpful in constructing improved MQC methods in the future.
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http://dx.doi.org/10.1063/5.0027799DOI Listing
December 2020

Radiative MHD Nanofluid Flow over a Moving Thin Needle with Entropy Generation in a Porous Medium with Dust Particles and Hall Current.

Entropy (Basel) 2020 Mar 18;22(3). Epub 2020 Mar 18.

Department of Computer Science and Engineering, Soonchunhyang University, Asan 31538, Korea.

This paper investigated the behavior of the two-dimensional magnetohydrodynamics (MHD) nanofluid flow of water-based suspended carbon nanotubes (CNTs) with entropy generation and nonlinear thermal radiation in a Darcy-Forchheimer porous medium over a moving horizontal thin needle. The study also incorporated the effects of Hall current, magnetohydrodynamics, and viscous dissipation on dust particles. The said flow model was described using high order partial differential equations. An appropriate set of transformations was used to reduce the order of these equations. The reduced system was then solved by using a MATLAB tool bvp4c. The results obtained were compared with the existing literature, and excellent harmony was achieved in this regard. The results were presented using graphs and tables with coherent discussion. It was comprehended that Hall current parameter intensified the velocity profiles for both CNTs. Furthermore, it was perceived that the Bejan number boosted for higher values of Darcy-Forchheimer number.
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http://dx.doi.org/10.3390/e22030354DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7516827PMC
March 2020

Radiography and ct features of atherosclerosis in two miniature schnauzer dogs.

J Vet Sci 2020 Nov;21(6):e89

College of Veterinary Medicine, Chonnam National University, Gwangju 61186, Korea.

Two miniature Schnauzer dogs with chronic pancreatitis were investigated. Both dogs showed systemic hypertension and increased concentrations of triglycerides and C-reactive protein. Abdominal radiography revealed cylindrical calcification in the retroperitoneum, and computed tomography (CT) showed extensive calcification of the abdominal and peripheral arteries in both dogs. Metastases and other dystrophic conditions that can cause arterial calcification were excluded based on the laboratory tests, and the dogs were diagnosed with atherosclerosis ante mortem. Atherosclerosis should be considered when extensive arterial calcification is observed on abdominal radiography or CT in miniature Schnauzers.
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http://dx.doi.org/10.4142/jvs.2020.21.e89DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7710456PMC
November 2020

Coupling cold plasma and membrane photobioreactor for enhanced fouling control during livestock excreta treatment.

Chemosphere 2021 Feb 20;265:129031. Epub 2020 Nov 20.

Department of Environment and Energy, Division of Civil, Environmental, Materials Resource, and Energy Engineering, Soil Environment Research Center, Jeonbuk National University, Jeollabuk-do, 54896, Republic of Korea. Electronic address:

To treat high-turbidity livestock excrements (LE), this study suggests a synergistic system coupling cold plasma (CP) and membrane photobioreactor (MPBR). During the continuous operation of the integrated system, physico-chemical oxidation of CP decompose turbidity and total suspended solids (TSS) up to 99.9%. The microalgal concentration of Scenedesmus obliquus in the following MPBR reach as high as 1,944 mg D.W./L, which indicates the residual organic and inorganic substances were actively consumed by phototrophic metabolism. Pearson correlation analysis confirms this synergistic relationship of turbidity and TSS with biological growth parameters such as biomass growth, soluble microbial products, and extracellular polymeric substances. Results evidence that the turbidity and TSS are directly connected to the microalgal growth in this integrated system thus the role of CP is crucial to achieving the LE treatment goal. Overall, this study provides a guideline to support the enhanced treatment strategy to control LE with the production of bioresources for sustainable carbon and nutrient cycles.
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http://dx.doi.org/10.1016/j.chemosphere.2020.129031DOI Listing
February 2021

Chromosomal assembly of the Antarctic toothfish ( ) genome using third-generation DNA sequencing and Hi-C technology.

Zool Res 2021 Jan;42(1):124-129

College of Life Sciences and Biotechnology, Korea University, Seoul 02841, Republic of Korea.

The Antarctic toothfish, , belongs to the Nototheniidae family and is distributed in sub-zero temperatures below S60° latitude in the Southern Ocean. Therefore, it is an attractive model species to study the stenothermal cold-adapted character state. In this study, we successfully generated highly contiguous genome sequences of , which contained 1 062 scaffolds with a N50 length of 36.98 Mb and longest scaffold length of 46.82 Mb. Repetitive elements accounted for 40.87% of the genome. We also inferred 32 914 protein-coding genes using gene prediction and transcriptome sequencing and detected splicing variants using Isoform-Sequencing (Iso-Seq), which will be invaluable resource for further exploration of the adaptation mechanisms of Antarctic toothfish. This new high-quality reference genome of provides a fundamental resource for a deeper understanding of cold adaptation and conservation of species.
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http://dx.doi.org/10.24272/j.issn.2095-8137.2020.264DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7840457PMC
January 2021

One-Stage Extension Shortening Osteotomy for Syndromic Camptodactyly.

J Clin Med 2020 Nov 20;9(11). Epub 2020 Nov 20.

Division of Pediatric Orthopaedic Surgery, Severance Children's Hospital, Yonsei University College of Medicine, Seoul 03722, Korea.

Syndromic camptodactyly often affects multiple fingers, and severe deformities are common compared to idiopathic camptodactyly. This study aimed to evaluate the use of a one-stage extension shortening osteotomy of the proximal phalanx for patients with syndromic camptodactyly without tendon surgery. Forty-nine cases of syndromic camptodactyly were included. Forty fingers (81.6%) were associated with arthrogryposis multiplex congenita, and nine (18.4%) with other syndromes. Six fingers presented with a moderate form (30° to 60°) of camptodactyly, whereas 43 fingers manifested the severe form (>60°). The mean age at the time of surgery was 8.5 years, and the patients were followed for a mean of 3.9 years. The mean length of the shortening of the proximal phalanx was 4.9 mm, which averaged 17.8% of the proximal phalanx's original preoperative length. The mean operative time was 25.8 min, and the PIP joint was fixed using Kirschner wires with an average flexion position of 7.6°. The mean flexion contracture improved from 76° preoperatively to 41° postoperatively. The mean preoperative active arc of motion was 23°, which improved to 49° postoperatively. A one-stage extension shortening osteotomy is a straightforward and effective technique for the improvement of finger function through the indirect lengthening of volar structures without the flexor tendon lengthening. The osteotomy could simultaneously correct bony abnormalities. This simple procedure is especially suitable for surgery on multiple fingers in patients with syndromic camptodactyly.
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http://dx.doi.org/10.3390/jcm9113731DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7699896PMC
November 2020

Comparison of human poly-N-acetyl-lactosamine synthase structure with GT-A fold glycosyltransferases supports a modular assembly of catalytic subsites.

J Biol Chem 2020 Nov 23. Epub 2020 Nov 23.

University of Georgia, United States.

Poly-N-acetyl-lactosamine (poly-LacNAc) structures are composed of repeating [-Galβ(1,4)-GlcNAcβ(1,3)-] glycan extensions. They are found on both N- and O--glycoproteins and glycolipids, and play an important role in development, immune function, and human disease. The majority of mammalian poly-LacNAc is synthesized by the alternating iterative action of β1,3-N-acetylglucosaminyltransferase 2 (B3GNT2) and β1,4-galactosyltransferases. B3GNT2 is in the largest mammalian glycosyltransferase family, GT31, but little is known about the structure, substrate recognition, or catalysis by family members. Here we report the structures of human B3GNT2 in complex with UDP:Mg, and in complex with both UDP:Mg and a glycan acceptor, lacto-N-neotetraose. The B3GNT2 structure conserves the GT-A fold and the DxD motif that coordinates a Mg ion for binding the UDP-GlcNAc sugar donor. The acceptor complex shows interactions with only the terminal Galβ(1,4)-GlcNAcβ(1,3)- disaccharide unit, which likely explains the specificity for both N- and O-glycan acceptors. Modeling of the UDP-GlcNAc donor supports a direct displacement inverting catalytic mechanism. Comparative structural analysis indicates that nucleotide sugar donors for GT-A fold glycosyltransferases bind in similar positions and conformations without conserving interacting residues, even for enzymes that use the same donor substrate. In contrast, the B3GNT2 acceptor binding site is consistent with prior models suggesting that the evolution of acceptor specificity involves loops inserted into the stable GT-A fold. These observations support the hypothesis that GT-A fold glycosyltransferases employ co-evolving donor, acceptor, and catalytic subsite modules as templates to achieve the complex diversity of glycan linkages in biological systems.
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http://dx.doi.org/10.1074/jbc.RA120.015305DOI Listing
November 2020

Is Computed Tomography Necessary for Diagnostic Workup in Displaced Pediatric Medial Epicondyle Fractures?

Diagnostics (Basel) 2020 Nov 17;10(11). Epub 2020 Nov 17.

Department of Orthopaedic Surgery, Gangnam Severance Hospital, Yonsei University College of Medicine, Seoul 06273, Korea.

This study aimed to compare the treatment outcomes and complications between operatively and nonoperatively treated medial epicondyle fractures with displacement of >5 mm as accurately measured on three-dimensional computed tomography (3D CT). We retrospectively reviewed 77 patients who had isolated medial epicondylar fractures with displacement of >5 mm. The mean age at injury was 11.4 years. Patients were assigned to one of two groups: 21 patients treated nonoperatively and 56 patients treated surgically. Additionally, patients treated operatively were divided into two subgroups according to fixation method; 31 patients underwent internal fixation with K-wires and 25 patients underwent internal fixation with a screw. Radiological and functional outcomes were compared among the three groups. Although the bony union rate was significantly higher in patients treated operatively compared to patients treated non-operatively (96.4% vs. 23.8%, < 0.001), there were no significant differences in functional outcomes between the two groups. In the nonoperative group, three patients underwent osteosynthesis for symptomatic nonunion. There were no significant differences in radiological and functional outcomes between the two subgroups divided by fixation method. In a pediatric medial epicondylar fracture with a displacement of >5 mm as accurately measured on 3D CT, despite the difference in union rate, there was no difference in functional outcomes between operative and nonoperative treatment. Performing CT only to measure the fracture displacement in obviously displaced medial epicondylar fracture is not considered as a part of the "necessary" diagnostic workups.
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http://dx.doi.org/10.3390/diagnostics10110957DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7696012PMC
November 2020

Surgical Treatment for a Broken Acupuncture Needle in the Retroperitoneal Space: A Case Report.

Korean J Neurotrauma 2020 Oct 23;16(2):332-336. Epub 2020 Sep 23.

Department of Neurosurgery, Dankook University Hospital, Cheonan, Korea.

Acupuncture is a well-known traditional medical procedure in Korea and is being increasingly used to treat various conditions including pain control, especially among the older generation. However, several problems related to acupuncture have been reported. A 27-year-old woman expressed that she had back pain occurring despite her posture. She received acupuncture therapy 2 months prior. We discovered a needle-shaped foreign material near the T12 vertebra body in the retroperitoneum. We extracted 2 pieces of deteriorated broken needles by laparoscopy. Postoperatively, the woman was discharged within 5 days with no complications. We report a patient that received laparoscopy with a full recovery after having severe back pain caused by a broken needle which was stuck in the retroperitoneum.
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http://dx.doi.org/10.13004/kjnt.2020.16.e31DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7607017PMC
October 2020

Treatment Experiences of Traumatic Brain Injury Patients using Doctor-Helicopter Emergency Medical Service: Early Data in a Regional Trauma Center.

Korean J Neurotrauma 2020 Oct 28;16(2):157-165. Epub 2020 Oct 28.

Department of Neurosurgery, Dankook University Hospital, Cheonan, Korea.

Objective: The purpose of this study is to analyze the results of doctor helicopter emergency medical service (HEMS) in traumatic brain injury (TBI) patients and to understand the effect and improvement of doctor HEMS.

Methods: We included TBI patients transferred by doctor HEMS of our hospital between February 2016 and December 2017. Basic characteristics, HEMS data, treatment and results data were analyzed retrospectively. We divided the patients into 3 groups as regarding severity of patient, relevance of treatment and transfer. We investigated the preventable trauma death rate (PTDR) of these groups to increase the reliability of the treatment outcome.

Results: TBI patients using doctor HEMS were indicated in 98 patients (18.7%) among 522 overall HEMS patients. The overall mortality was consisted in 21.4% and 43.2% was resulted in Glasgow outcome scale 4 or 5. The group of proper transport and treatment for severe TBI was consisted in 62.2% including 13 mortality cases and no preventable death. The group of delayed transport or treatment for severe TBI was 18.3% including 8 mortality cases and 1 preventable death. The PTDR of TBI after doctor HEMS was significantly lower than that of overall TBI (4.8% vs. 11.6%, =0.045).

Conclusion: In patients with severe TBI, doctor HEMS can improve treatment outcomes by reducing treatment delay and unnecessary examinations and this result was evidenced that the PTDR were decreased significantly after doctor HEMS transport. The appropriate treatment is mandatory for real-time communication with the emergency doctor and treatment preparation of the trauma team during the HEMS transport.
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http://dx.doi.org/10.13004/kjnt.2020.16.e50DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7607028PMC
October 2020