Publications by authors named "Huiting Zhang"

76 Publications

Optimal Performance and Application for Seagull Optimization Algorithm Using a Hybrid Strategy.

Entropy (Basel) 2022 Jul 14;24(7). Epub 2022 Jul 14.

Communication and Network Laboratory, Dalian University, Dalian 116622, China.

This paper aims to present a novel hybrid algorithm named SPSOA to address problems of low search capability and easy to fall into local optimization of seagull optimization algorithm. Firstly, the Sobol sequence in the low-discrepancy sequences is used to initialize the seagull population to enhance the population's diversity and ergodicity. Then, inspired by the sigmoid function, a new parameter is designed to strengthen the ability of the algorithm to coordinate early exploration and late development. Finally, the particle swarm optimization learning strategy is introduced into the seagull position updating method to improve the ability of the algorithm to jump out of local optimization. Through the simulation comparison with other algorithms on 12 benchmark test functions from different angles, the experimental results show that SPSOA is superior to other algorithms in stability, convergence accuracy, and speed. In engineering applications, SPSOA is applied to blind source separation of mixed images. The experimental results show that SPSOA can successfully realize the blind source separation of noisy mixed images and achieve higher separation performance than the compared algorithms.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.3390/e24070973DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9317883PMC
July 2022

Quantitative analysis of mean apparent propagator-magnetic resonance imaging for distinguishing glioblastoma from solitary brain metastasis.

Eur J Radiol 2022 Jul 3;154:110430. Epub 2022 Jul 3.

Department of Magnetic Resonance Imaging, the First Affiliated Hospital of Zhengzhou University, Zhengzhou 450052, China. Electronic address:

Purpose: Distinguishing glioblastoma (GBM) and solitary brain metastasis (SBM) is vital for determining the optimal treatment. GBM and SBM present similar imaging characteristics on conventional magnetic resonance imaging (MRI). The aim of this study was to evaluate the efficacy of quantitative analysis of mean apparent propagator (MAP)-MRI for distinguishing GBM and SBM.

Method: Eighty-nine patients were enrolled. Regions of interest (ROIs), including the enhancing area (EA), peritumoural high signal intensity area (PHA), and maximum abnormal signal area (MASA), were manually delineated. The following MAP parameters for each region were measured: mean square displacement (MSD), non-Gaussianity (NG), NG axial (NGAx), NG vertical, Q-space inverse variance, return to origin probability (RTOP), return to axis probability (RTAP), and return to plane probability (RTPP). Normalised MAP values from each region were compared between the GBM and SBM groups, and their diagnostic efficiency was assessed. Multivariate logistic regression analysis was used to create the most accurate model.

Results: Compared with the SBM group, the MSD was significantly lower in the GBM group, whereas the RTAP, RTOP, and RTPP were significantly higher in each region, except for RTAP (all P < 0.05). RTPP, MSD, and RTPP showed the most significant differences (all P < 0.01). The best logistic regression model combined RTPP, MSD, and NGAx (area under the curve, 0.840).

Conclusions: Quantitative analysis of MAP-MRI is useful for distinguishing GBM from SBM. Multivariate analysis combined with multiple ROIs can improve diagnostic performance.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.ejrad.2022.110430DOI Listing
July 2022

Optimization of scan parameters to reduce acquisition time for RESOLVE-based diffusion kurtosis imaging (DKI) in nasopharyngeal carcinoma (NPC).

Br J Radiol 2022 Aug 22;95(1136):20210641. Epub 2022 Jun 22.

Department of Radiology, Hubei Cancer Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, PR China.

Objective: To shorten acquisition time of readout segmentation of long variable echo trains (RESOLVE)-based diffusion kurtosis imaging (DKI) via Readout Partial Fourier (RPF) and b-value combinations.

Methods: The RESOLVE-based DKI images of 38 patients with nasopharyngeal carcinoma (NPC) were prospectively enrolled. For RESOLVE-based DKI images with 5/8 RPF and without RPF, objective and subjective evaluations of image quality were performed. A total of nine groups with different b-value combinations were simulated, and the influence of different b-value combinations for RESOLVE-RPF-based DKI sequences was assessed using the intraclass correlation coefficient (ICC).

Results: The mean values of signal-to-noise ratio (SNR) and contrast-to-noise ratio (CNR) in DKI images without RPF were higher than those with 5/8 RPF (252.9 ± 77.7 vs 247.3 ± 85.5 and 5.8 ± 2.8 vs 5.4 ± 2.3, respectively), but not significantly ( = 0.460 and = 0.180, respectively). In comparing the ICCs between nine groups of different b-value combinations in RESOLVE-RPF-based DKI, group (200, 800, 2000 s/mm), group (200, 400, 800, 2000 s/mm) and group (200, 800, 1500, 2000 s/mm) were not significantly different ( > 0.001) and showed excellent agreement (0.81-1.00) with that of group (200, 400, 800, 1500, 2000 s/mm). Using b-value optimization and RPF technology, the group with RPF (200, 400, 800, 2000 s/mm) showed a 56% reduced scanning compared with the group without RPF (200, 400, 800, 1500, 2000 s/mm; 3 min 46 s 8 min 31 s, respectively).

Conclusion: DKI with RPF did not significantly affect image quality, but both RPF and different b-value combinations can affect the scanning time. The combination of RPF and b-value optimization can ensure the stability of DKI parameters and reduce the scanning time by 56%.

Advances In Knowledge: This work is to optimize scan parameters, RPF and b-value combinations, to reduce acquisition time for RESOLVE-based DKI in NPC. To our knowledge, the effect of RESOLVE-RPF and b-value combinations on DKI has not been reported.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1259/bjr.20210641DOI Listing
August 2022

Ketogenic Diet Therapy for Drug-Resistant Epilepsy and Cognitive Impairment in Children With Tuberous Sclerosis Complex.

Front Neurol 2022 24;13:863826. Epub 2022 May 24.

Department of Neurology, Shenzhen Children's Hospital, Shenzhen, China.

Objective: Tuberous sclerosis complex (TSC) is a rare disease with a high risk of epilepsy and cognitive impairment in children. Ketogenic diet (KD) therapy has been consistently reported to be beneficial to TSC patients. In this study, we aimed to investigate the efficacy and safety of KD in the treatment of drug-resistant epilepsy and cognitive impairment in children with TSC.

Methods: In this multicenter study, 53 children (33 males and 20 females) with drug-resistant epilepsy or cognitive impairment caused by TSC were retrospectively recruited from 10 hospitals from January 1, 2010, to December 31, 2020. Intention-to-treat analysis was used to evaluate seizure reduction and cognition improvement as outcomes after KD therapy.

Results: Of the 53 TSC patients included, 51 failed to be seizure-free with an average of 5.0 (range, 4-6) different anti-seizure medications (ASMs), before KD therapy. Although the other two patients achieved seizure freedom before KD, they still showed psychomotor development delay and electroencephalogram (EEG) abnormalities. At 1, 3, 6, and 12 months after the KD therapy, 51 (100%), 46 (90.2%), 35 (68.6%), and 16 patients (31.4%) remained on the diet therapy, respectively. At these time points, there were 26 (51.0%), 24 (47.1%), 22 (43.1%) and 13 patients (25.5%) having ≥50% reductions in seizure, including 11 (21.6%), 12 (23.5%), 9 (17.6%) and 3 patients (5.9%) achieving seizure freedom. In addition, of 51 patients with psychomotor retardation, 36 (36 of 51, 70.6%) showed cognitive and behavioral improvements. During the KD therapy, no serious side effects occurred in any patient. The most common side effects were gastrointestinal disturbance (20 of 53, 37.7%) and hyperlipidemia (6 of 53, 11.3%). The side effects were gradually relieved after adjustment of the ketogenic ratio and symptomatic treatment.

Conclusion: KD is an effective and safe treatment for TSC-related drug-resistant epilepsy and cognitive impairment in children. KD can reduce seizure frequency and may potentially improve cognition and behavior.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.3389/fneur.2022.863826DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9171393PMC
May 2022

Blind Source Separation Based on Double-Mutant Butterfly Optimization Algorithm.

Sensors (Basel) 2022 May 24;22(11). Epub 2022 May 24.

Communication and Network Laboratory, Dalian University, Dalian 116622, China.

The conventional blind source separation independent component analysis method has the problem of low-separation performance. In addition, the basic butterfly optimization algorithm has the problem of insufficient search capability. In order to solve the above problems, an independent component analysis method based on the double-mutant butterfly optimization algorithm (DMBOA) is proposed in this paper. The proposed method employs the kurtosis of the signal as the objective function. By optimizing the objective function, blind source separation of the signals is realized. Based on the original butterfly optimization algorithm, DMBOA introduces dynamic transformation probability and population reconstruction mechanisms to coordinate global and local search, and when the optimization stagnates, the population is reconstructed to increase diversity and avoid falling into local optimization. The differential evolution operator is introduced to mutate at the global position update, and the sine cosine operator is introduced to mutate at the local position update, hence, enhancing the local search capability of the algorithm. To begin, 12 classical benchmark test problems were selected to evaluate the effectiveness of DMBOA. The results reveal that DMBOA outperformed the other benchmark algorithms. Following that, DMBOA was utilized for the blind source separation of mixed image and speech signals. The simulation results show that the DMBOA can realize the blind source separation of an observed signal successfully and achieve higher separation performance than the compared algorithms.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.3390/s22113979DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9182827PMC
May 2022

In Vitro Investigation on the Effect of Dendrobine on the Activity of Cytochrome P450 Enzymes.

Planta Med 2022 May 6. Epub 2022 May 6.

Department of Rehabilitation Medicine, Xingtai People's Hospital, Hebei Medical University Affiliated Hospital, Xingtai, Hebei, China.

Dendrobine is the major active ingredient of , and , all of which are used in traditional Chinese medicine owing to their antitumor and anti-inflammation activities. Hence, investigation on the interaction of dendrobine with cytochrome P450 enzymes could provide a reference for the clinical application of . The effects of dendrobine on cytochrome P450 enzymes activities were investigated in the presence of 0, 2.5, 5, 10, 25, 50, and 100 µM dendrobine in pooled human liver microsomes. The specific inhibitors were employed as the positive control and the blank groups were set as the negative control. The Lineweaver-Burk plots were plotted to characterize the specific inhibition model and obtain the kinetic parameters. The study reveals that dendrobine significantly inhibited the activity of CYP3A4, 2C19, and 2D6 with IC values of 12.72, 10.84, and 15.47 µM, respectively. Moreover, the inhibition of CYP3A4 was found to be noncompetitive ( = 6.41 µM) and time dependent ( = 2.541 µM,  = 0.0452 min), while the inhibition of CYP2C19 and 2D6 was found to be competitive with the values of 5.22 and 7.78 µM, respectively, and showed no time-dependent trends. The inhibitory effect of dendrobine implies the potential drug-drug interaction between dendrobine and CYP3A4-, 2C9-, and 2D6-metabolized drugs. Nonetheless, these findings need further validation.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1055/a-1806-2935DOI Listing
May 2022

Diagnostic performance of gliomas grading and IDH status decoding A comparison between 3D amide proton transfer APT and four diffusion-weighted MRI models.

J Magn Reson Imaging 2022 Apr 30. Epub 2022 Apr 30.

Department of Neurosurgery, The Second Xiangya Hospital, Central South University, No. 139 Middle Renmin Rd, Changsha, Hunan Province, 410011, P.R. China.

Background: The focus of neuro-oncology research has changed from histopathologic grading to molecular characteristics, and medical imaging routinely follows this change.

Purpose: To compare the diagnostic performance of amide proton transfer (APT) and four diffusion models in gliomas grading and isocitrate dehydrogenase (IDH) genotype.

Study Type: Prospective.

Population: A total of 62 participants (37 males, 25 females; mean age, 52 ± 13 years) whose IDH genotypes were mutant in 6 of 14 grade II gliomas, 8 of 20 of grade III gliomas, and 4 of 28 grade IV gliomas.

Field Strength/sequence: APT imaging using sampling perfection with application optimized contrasts by using different flip angle evolutions (SPACE) and DWI with q-space Cartesian grid sampling were acquired at 3 T.

Assessment: The ability of diffusion kurtosis imaging, diffusion kurtosis imaging, neurite orientation dispersion and density imaging (NODDI), mean apparent propagator (MAP), and APT imaging for glioma grade and IDH status were assessed, with histopathological grade and genetic testing used as a reference standard. Regions of interest (ROIs) were drawn by two neuroradiologists after consensus.

Statistical Tests: T-test and Mann-Whitney U test; one-way analysis of variance (ANOVA); receiver operating curve (ROC) and area under the curve (AUC); DeLong test. P value < 0.05 was considered statistically significant.

Results: Compared with IDH-mutant gliomas, IDH-wildtype gliomas showed a significantly higher mean, 5th-percentile (APT ), and 95th-percentile from APTw, the 95th-percentile value of axial, mean, and radial diffusivity from DKI, and 95th-percentile value of isotropic volume fraction from NODDI, and no significantly different parameters from DTI and MAP (P = 0.075-0.998). The combined APT model showed a significantly wider area under the curve (AUC 0.870) for IDH status, when compared with DKI and NODDI. APT was significantly different between two of the three groups (glioma II vs. glioma III vs. glioma IV: 1.35 ± 0.75 vs. 2.09 ± 0.93 vs. 2.71 ± 0.81).

Data Conclusion: APT has higher diagnostic accuracy than DTI, DKI, MAP, and NODDI in glioma IDH genotype. APT can effectively identify both tumor grading and IDH genotyping, making it a promising biomarker for glioma classification.

Evidence Level: 1 TECHNICAL EFFICACY: Stage 2.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1002/jmri.28211DOI Listing
April 2022

Case Report: Identification of Compound Heterozygous Mutations in a Patient With Late-Onset Glycogen Storage Disease Type II (Pompe Disease).

Front Neurol 2022 21;13:839263. Epub 2022 Mar 21.

Guangdong Key Laboratory of Age-Related Cardiac and Cerebral Diseases, Department of Neurology, Institute of Neurology, Affiliated Hospital of Guangdong Medical University, Zhanjiang, China.

Pompe disease is an autosomal recessive hereditary lysosomal disorder and correlated with acid α-glucosidase enzyme (GAA) deficiencies, which lead to accumulation of glycogen in all tissues, most notably in skeletal muscles. Adult late-onset Pompe disease (LOPD) is a slowly progressive disease of proximal myopathy with later involvement of the respiratory muscles, resulting in respiratory failure. In this study, we reported a 22-year-old Chinese woman with inability to withstand heavy physical activity since childhood, who presented with respiratory and ambulation weakness in 2 months. On admission, her bilateral upper limbs strength was 4/5 and lower limbs strength was 3/5 according to Medical Research Council (MRC) score. The patient had compound heterozygotes containing a newly identified 4 nt deletion of coding sequence (deletion nt 1411_1414) in one of the acid α-glucosidase alleles and a c.2238G>C (p.Trp746Cys) missense mutation. This deletion has been reported in infant-onset Pompe disease (IOPD) but not LOPD. Intriguingly, this deletion mutation was not found in the patient's family and was considered as pathogenic. Muscle biopsy showed scattered vacuoles with basophilic granules inside the subsarcolemmal area, which were strongly stained by periodic acid-Schiff (PAS). Laboratory tests revealed a significant increase of creatine kinase MB isoenzyme (CK-MB) and lactate dehydrogenase (LDH). GAA level was 9.77 nmol/1 h/mg and was not sufficient for the diagnosis of GAA activity deficiency (0-3.78 nmol/1 h/mg). In summary, mutational analysis of GAA and muscle biopsy are crucial in the diagnosis of Pompe disease.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.3389/fneur.2022.839263DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8977516PMC
March 2022

G-CSF: A vehicle for communication between trophoblasts and macrophages which may cause problems in recurrent spontaneous abortion.

Placenta 2022 04 26;121:164-172. Epub 2022 Mar 26.

Department of Gynecology and Obstetrics, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, 430030, China. Electronic address:

Introduction: The etiology of approximately half of patients with recurrent spontaneous abortion (RSA) has yet to be established. Granulocyte-colony stimulating factor (G-CSF) exerts a protective effect on pregnancy and its absence may lead to pregnancy failure. However, the effects and mechanisms of G-CSF activities have not been fully explored. Therefore, we aimed at evaluating whether a loss of G-CSF induces RSA by affecting cell communication at the maternal-foetal interface.

Methods: Villous and decidual tissues were obtained from participants and expression levels of G-CSF determined by qRT-PCR, Western blot and immunohistochemistry. G-CSF levels in trophoblasts were downregulated by siRNA. Exosomes were extracted from trophoblasts and co-cultured with macrophages. Molecular expression levels of key genes were determined by qRT-PCR and Western blot. Migration and proliferation of cells were evaluated by transwell and CCK8 assays. The RSA mice models were intraperitoneally administered with G-CSF to assess pregnancy outcomes and expression profiles of G-CSF as well as its receptor at the mother-foetal interface.

Results: Relative to the decidua, G-CSF was highly expressed in the villus, and expression levels were low in RSA tissues compared to normal tissues. Down-regulation of G-CSF in the trophoblast cell line (HTR-8/SVneo) by siRNA was associated with a decrease in cell activities. Trophoblast-derived exosomes inhibited the activation of the macrophage cell line (RAW264.7), whereas G-CSF free exosomes had no effects on macrophage activation. Intraperitoneal administration of G-CSF improved pregnancy outcomes in RSA mice and increased the amounts of G-CSF at the maternal-foetal interface.

Discussion: G-CSF levels were downregulated in villi of RSA patients. The absence of G-CSF impaired the proliferation as well as migration capacities of trophoblasts, and weakened the suppression of trophoblasts against macrophages. This implies that suppressed G-CSF levels may be a key factor in RSA occurrence. G-CSF decreased the rate of abortion in RSA mice, thus, it could be a treatment option for RSA patients.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.placenta.2022.03.125DOI Listing
April 2022

White Matter Abnormalities and Cognitive Deficit After Mild Traumatic Brain Injury: Comparing DTI, DKI, and NODDI.

Front Neurol 2022 10;13:803066. Epub 2022 Mar 10.

Department of Radiology, The Second Xiangya Hospital, Central South University, Changsha, China.

White matter (WM) disruption is an important determinant of cognitive impairment after mild traumatic brain injury (mTBI), but traditional diffusion tensor imaging (DTI) shows some limitations in assessing WM damage. Diffusion kurtosis imaging (DKI) and neurite orientation dispersion and density imaging (NODDI) show advantages over DTI in this respect. Therefore, we used these three diffusion models to investigate complex WM changes in the acute stage after mTBI. From 32 mTBI patients and 31 age-, sex-, and education-matched healthy controls, we calculated eight diffusion metrics based on DTI (fractional anisotropy, axial diffusivity, radial diffusivity, and mean diffusivity), DKI (mean kurtosis), and NODDI (orientation dispersion index, volume fraction of intracellular water (Vic), and volume fraction of the isotropic diffusion compartment). We used tract-based spatial statistics to identify group differences at the voxel level, and we then assessed the correlation between diffusion metrics and cognitive function. We also performed subgroup comparisons based on loss of consciousness. Patients showed WM abnormalities and cognitive deficit. And these two changes showed positive correlation. The correlation between Vic of the splenium of the corpus callosum and Digit Symbol Substitution Test scores showed the smallest -value ( = 0.000, = 0.481). We concluded that WM changes, especially in the splenium of the corpus callosum, correlate to cognitive deficit in this study. Furthermore, the high voxel count of NODDI results and the consistency of mean kurtosis and the volume fraction of intracellular water in previous studies and our study showed the functional complementarity of DKI and NODDI to DTI.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.3389/fneur.2022.803066DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8960262PMC
March 2022

Comprehensive Risk System Based on Shear Wave Elastography and BI-RADS Categories in Assessing Axillary Lymph Node Metastasis of Invasive Breast Cancer-A Multicenter Study.

Front Oncol 2022 10;12:830910. Epub 2022 Mar 10.

Department of Ultrasound, Beijing Friendship Hospital, Capital Medical University, Beijing, China.

Purpose: To develop a risk stratification system that can predict axillary lymph node (LN) metastasis in invasive breast cancer based on the combination of shear wave elastography (SWE) and conventional ultrasound.

Materials And Methods: A total of 619 participants pathologically diagnosed with invasive breast cancer underwent breast ultrasound examinations were recruited from a multicenter of 17 hospitals in China from August 2016 to August 2017. Conventional ultrasound and SWE features were compared between positive and negative LN metastasis groups. The regression equation, the weighting, and the counting methods were used to predict axillary LN metastasis. The sensitivity, specificity, and the areas under the receiver operating characteristic curve (AUC) were calculated.

Results: A significant difference was found in the Breast Imaging Reporting and Data System (BI-RADS) category, the "stiff rim" sign, minimum elastic modulus of the internal tumor and peritumor region of 3 mm between positive and negative LN groups ( < 0.05 for all). There was no significant difference in the diagnostic performance of the regression equation, the weighting, and the counting methods (p > 0.05 for all). Using the counting method, a 0-4 grade risk stratification system based on the four characteristics was established, which yielded an AUC of 0.656 (95% CI, 0.617-0.693, p < 0.001), a sensitivity of 54.60% (95% CI, 46.9%-62.1%), and a specificity of 68.99% (95% CI, 64.5%-73.3%) in predicting axillary LN metastasis.

Conclusion: A 0-4 grade risk stratification system was developed based on SWE characteristics and BI-RADS categories, and this system has the potential to predict axillary LN metastases in invasive breast cancer.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.3389/fonc.2022.830910DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8960926PMC
March 2022

Trends and hot topics in radiology, nuclear medicine and medical imaging from 2011-2021: a bibliometric analysis of highly cited papers.

Jpn J Radiol 2022 Aug 28;40(8):847-856. Epub 2022 Mar 28.

Department of Medical Imaging, Suizhou Central Hospital, Hubei University of Medicine, No.60 Longmen Street, Jiefang Road, Suizhou, 441399, Hubei, China.

Purpose: To spotlight the trends and hot topics looming from the highly cited papers in the subject category of Radiology, Nuclear Medicine & Medical Imaging with bibliometric analysis.

Materials And Methods: Based on the Essential Science Indicators, this study employed a bibliometric method to examine the highly cited papers in the subject category of Radiology, Nuclear Medicine & Medical Imaging in Web of Science (WoS) Categories, both quantitatively and qualitatively. In total, 1325 highly cited papers were retrieved and assessed spanning from the years of 2011 to 2021. In particular, the bibliometric information of the highly cited papers based on WoS database such as the main publication venues, the most productive countries, and the top cited publications was presented. An Abstract corpus was built to help identify the most frequently explored topics. VoSviewer was used to visualize the co-occurrence networks of author keywords.

Results: The top three active journals are Neuroimage, Radiology and IEEE T Med Imaging. The United States, Germany and England have the most influential publications. The top cited publications unrelated to COVID-19 can be grouped in three categories: recommendations or guidelines, processing software, and analysis methods. The top cited publications on COVID-19 are dominantly in China. The most frequently explored topics based on the Abstract corpus and the author keywords with the great link strengths overlap to a great extent. Specifically, phrases such as magnetic resonance imaging, deep learning, prostate cancer, chest CT, computed tomography, CT images, coronavirus disease, convolutional neural network(s) are among the most frequently mentioned.

Conclusion: The bibliometric analysis of the highly cited papers provided the most updated trends and hot topics which may provide insights and research directions for medical researchers and healthcare practitioners in the future.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1007/s11604-022-01268-zDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8958482PMC
August 2022

Clinical application of MIIRMR as a salvage method in gadolinium-enhanced MRI after intra-tympanic injection.

Acta Otolaryngol 2022 Mar-Apr;142(3-4):241-247. Epub 2022 Mar 18.

Department of Otorhinolaryngology-Head and Neck Surgery, The First Affiliated Hospital of Guangxi Medical University, Nanning, China.

Background: Three-dimensional fluid-attenuated inversion-recovery (3 D-FLAIR) and real inversion-recovery (3 D-real IR) sequences are used to detect endolymphatic hydrops (EH), but medium inversion-time inversion-recovery imaging with magnitude reconstruction (MIIRMR) may be more sensitive.

Aims: We investigated the inner-ear visualisation success rate and EH detection rates of 3 D-FLAIR and 3 D-real IR, and whether salvage MIIRMR could improve EH detection.

Materials And Methods: Fifty-one patients (102 ears) with episodic or chronic vestibular syndrome were injected intra-tympanically with 8-fold diluted gadolinium, and 3 D-FLAIR and 3 D-real IR images obtained 24-h post-injection. If 3 D-FLAIR inner-ear visualisation failed, additional MIIRMR was performed. The success and EH detection rate increase by MIIRMR was calculated. The diagnostic performance of combined MIIRMR + 3D-FLAIR + 3D-real IR for Meniere's disease (MD) was evaluated.

Results: The success rates of 3 D-FLAIR and 3 D-real IR were 88.90% and 72.55%, respectively. MIIRMR increased the success and EH detection rates by 11.10% and 6.86%, respectively. In MD, MIIRMR increased these rates by 10.53% and 10.53%, respectively. 3 D-FLAIR + 3D-real IR + MIIRMR had 92.11% sensitivity, 79.68% specificity, 72.92% positive-predictive value, and 94.44% negative-predictive value for MD diagnosis.

Conclusion And Significance: MIIRMR can improve success and EH detection rates when 3 D-FLAIR fails. Combined MIIRMR + 3D-FLAIR + 3D-real IR is more valuable for diagnosing MD than conventional sequences.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1080/00016489.2022.2050806DOI Listing
May 2022

Multi-parameter diffusion and perfusion magnetic resonance imaging and radiomics nomogram for preoperative evaluation of aquaporin-1 expression in rectal cancer.

Abdom Radiol (NY) 2022 04 15;47(4):1276-1290. Epub 2022 Feb 15.

Department of Radiology, First Affiliated Hospital of Guangxi Medical University, Nanning, 530021, China.

Purpose: The overexpression of aquaporin-1 (AQP1) is associated with poor prognosis in rectal cancer. This study aimed to explore the value of multi-parameter diffusion and perfusion MRI and radiomics models in predicting AQP1 high expression.

Methods: This prospective study was performed from July 2019 to February 2021, which included rectal cancer participants after preoperative rectal MRI, with diffusion-weighted imaging, intravoxel incoherent motion (IVIM), diffusion kurtosis imaging (DKI), and dynamic contrast-enhanced (DCE) sequences. Radiomic features were extracted from MR images, and immunohistochemical tests assessed AQP1 expression. Selected quantitative MRI and radiomic features were analyzed. Receiver operating characteristic (ROC) curves evaluated the predictive performance. The nomogram performance was evaluated by its calibration, discrimen, and clinical utility. The intraclass correlation coefficient evaluated the interobserver agreement for the MRI features.

Results: 110 participants with the age of 60.7 ± 12.5 years been enrolled in this study. The apparent diffusion coefficient (ADC), IVIM_D, DKI_diffusivity, and DCE_Ktrans were significantly higher in participants with high AQP1 expression than in those with low expression (P < 0.05). ADC (b = 1000, 2000, and 3000 s/mm), IVIM_D, DKI_diffusivity, and DCE_Ktrans were positively correlated (r = 0.205, 0.275, 0.37, 0.235, 0.229, and 0.227, respectively; P < 0.05), whereas DKI_Kurtosis was negatively correlated (r = - 0.22, P = 0.021) with AQP1 expression. ADC (b = 3000 s/mm), IVIM_D, DKI_ diffusivity, DKI_Kurtosis, and DCE_Ktrans had moderate diagnostic efficiencies for high AQP1 expression (AUC = 0.715, 0.636, 0.627, 0.633, and 0.632, respectively; P < 0.05). The radiomic features had excellent predictive efficiency for high AQP1 expression (AUC = 0.967 and 0.917 for training and validation). The model-based nomogram had C-indexes of 0.932 and 0.851 for the training and validation cohorts, which indicated good fitting to the calibration curves (p > 0.05).

Conclusion: Diffusion and perfusion MRI can indicate the aquaporin-1 expression in rectal cancer, and radiomic features can enhance the predictive efficiency for high AQP1 expression. A nomogram for high aquaporin-1 expression will improve clinical decision-making.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1007/s00261-021-03397-xDOI Listing
April 2022

Differential Tractography and Correlation Tractography Findings on Patients With Mild Traumatic Brain Injury: A Pilot Study.

Front Hum Neurosci 2022 27;16:751902. Epub 2022 Jan 27.

Department of Radiology, The Second Xiangya Hospital of Central South University, Changsha, China.

Differential tractography and correlation tractography are new tractography modalities to study neuronal changes in brain diseases, but their performances in detecting neuronal injuries are yet to be investigated in patients with mild traumatic brain injury (mTBI). Here we investigated the white matter injury in mTBI patients using differential and correlation tractography. The diffusion MRI was acquired at 33 mTBI patients and 31 health controls. 7 of the mTBI patients had one-year follow-up scans, and differential tractography was used to evaluate injured fiber bundles on these 7 patients. All subjects were evaluated using digital symbol substitution test (DSST) and trail making test A (TMT-A), and the correlation tractography was performed to explore the exact pathways related to the cognitive performance. Our results showed that differential tractography revealed neuronal changes in the corpus callosum in all 7 follow-up mTBI patients with FDR between 0.007 and 0.17. Further, the correlation tractography showed that the splenium of the corpus callosum, combined with the right superior longitudinal fasciculus and right cingulum, were correlated with DSST (FDR = 0.001669) in the acute mTBI patients. The cognitive impairment findings in the acute stage and the longitudinal findings in the corpus callosum in the chronic stage of mTBI patients suggest that differential tractography and correlation tractography are valuable tools in the diagnostic and prognostic evaluation of neuronal injuries in mTBI patients.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.3389/fnhum.2022.751902DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8811572PMC
January 2022

Phenotypes in Children With Encephalopathy in China.

Front Neurosci 2021 2;15:761473. Epub 2021 Dec 2.

Department of Neurology, Shenzhen Children's Hospital, Shenzhen, China.

We aimed to explore the associated clinical phenotype and the natural history of patients with gene variations during early childhood and to identify their genotype-phenotype correlations. This study used a cohort of 13 patients with epilepsy and developmental disorder due to mutations, namely, 7 patients from Shenzhen Children's Hospital between September 2014 and January 2020 and 6 patients from previously published studies. Their clinical data were studied. A total of 13 children with gene variants (eight boys and five girls) were identified. The age of disease onset was in infancy. Mutations were located between exons 8 and 15; most were frameshift or truncated mutations. Four mutation sites (c.924G > A, c.1532-2_1532del, c.1747_1755dup, and c.1735_1738del) had not been reported before. All patients had global developmental delay within the first year of life, and intellectual impairment became gradually apparent. Some of them developed behavioral problems. The developmental delay occurred before the onset of seizures. All seven patients in our cohort presented with epilepsy; myoclonic seizures, absence seizures, and epileptic spasms were the most common seizure types. Abnormal electroencephalograms were identified from five patients before the onset of their seizures. All patients suffered from drug-resistance seizures. However, comorbidities such as behavioral problems were less frequently observed. The most common age of disease onset in gene mutations is in infancy, while neurodevelopmental delay and epilepsy are the major phenotypes. They have a higher percentage of drug-resistant epilepsy and epileptic spasms than those in previous reports. We should give attention to the patients with abnormal EEGs without seizures and think about the suitable time of the anti-seizure medications for them. We have not found the genotype-phenotype correlation. Chinese Clinical Trial Registry, Registration number: ChiCTR2100049289 (https://www.chictr.org.cn/listbycreater.aspx).
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.3389/fnins.2021.761473DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8678593PMC
December 2021

Multi-echo Dixon and breath-hold T2-corrected multi-echo single-voxel MRS for quantifying hepatic iron overload in rabbits.

Acta Radiol 2021 Dec 14:2841851211063007. Epub 2021 Dec 14.

Radiology Department, 117742The First Affiliated Hospital of Guangxi Medical University, Nanning, Guangxi, PR China.

Background: Three-dimensional (3D) multi-echo-Dixon (ME-Dixon) and breath-hold T2-corrected multi-echo single-voxel MR spectroscopy (HISTO) can simultaneously quantify liver fat and liver iron. However, their diagnostic efficacy and application scope for quantitative iron in co-existing fatty liver have not been adequately evaluated.

Purpose: To evaluate the accuracy of ME-Dixon and HISTO for quantitative analysis of hepatic iron in rabbits with iron deposition and fatty liver using liver-iron concentration (LIC) as a reference standard.

Material And Methods: ME-Dixon, HISTO, and conventional two-dimensional multi-echo gradient echo (GRE) sequences were performed on 42 rabbits. The following parameters were calculated: R2* from ME-Dixon and GRE; proton density fat fraction (PDFF) from the ME-Dixon, HISTO (normal TE range), and HISTO-H (extended TE range); and R2_water from HISTO and HISTO-H. The LIC and liver-fat concentration (LFC) were measured through chemical analysis, and their relationship with the MRI parameters were assessed. Receiver operating characteristic (ROC) curves and the area under the curve (AUC) were used to evaluate the diagnostic efficiency.

Results: LIC was significantly correlated with R2_HISTO-H, R2*_Dixon, and R2*_GRE ( = 0.858, 0.910, 0.931, respectively; < 0.001) and weakly with R2_HISTO ( = 0.424;  = 0.008). A strong correlation was also observed between the LFC and PDFF obtained from HISTO, HISTO-H, and ME-Dixon ( = 0.776, 0.811, 0.888, respectively; < 0.001). ME-Dixon showed the best performance with moderate iron overload (AUC = 0.983).

Conclusion: 3D ME-Dixon is useful for quantifying the LIC, especially with co-existing fatty liver. Its diagnostic performance is also superior to that of the HISTO sequence.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1177/02841851211063007DOI Listing
December 2021

Correction to: Upregulation of IL-15 in the placenta alters trophoblasts behavior contributing to gestational diabetes mellitus.

Cell Biosci 2021 Dec 13;11(1):207. Epub 2021 Dec 13.

National Clinical Research Center of Gynecology and Obstetrics, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, 1095 Jiefang Anv, Wuhan, 430030, Hubei, China.

View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1186/s13578-021-00714-1DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8670286PMC
December 2021

Whole-Tumor Histogram Analysis of Multiple Diffusion Metrics for Glioma Genotyping.

Radiology 2022 03 7;302(3):652-661. Epub 2021 Dec 7.

From the Department of MRI, the First Affiliated Hospital of Zhengzhou University, Zhengzhou, China (A.G., Q.C., E.G., J.Q., J.B., Y.Z., J.C.); and Department of MR Scientific Marketing, Siemens Healthineers, Shanghai, China (H.Z., X.Y., S.W.).

Background The isocitrate dehydrogenase genotype and codeletion status are key molecular markers included in glioma pathologic diagnosis. Advanced diffusion models provide additional microstructural information. Purpose To compare the diagnostic performance of histogram features of multiple diffusion metrics in predicting glioma and genotyping. Materials and Methods In this prospective study, participants were enrolled from December 2018 to December 2020. Diffusion-weighted imaging was performed by using a spin-echo echo-planar imaging sequence with five values (500, 1000, 1500, 2000, and 2500 sec/mm) in 30 directions for every value and one value of 0. Diffusion metrics of diffusion-tensor imaging (DTI), diffusion-kurtosis imaging (DKI), neurite orientation dispersion and density imaging (NODDI), and mean apparent propagator (MAP) were calculated, and their histogram features were analyzed in regions that included the entire tumor and peritumoral edema. Comparisons between groups were performed according to genotype and codeletion status. Logistic regression analysis was used to predict the and genotypes. Results A total of 215 participants (115 men, 100 women; mean age, 48 years ± 13 [standard deviation]) with grade II ( = 68), grade III ( = 35), and grade IV ( = 112) glioma were included. Among the DTI, DKI, NODDI, MAP, and total diffusion models, there were no significant differences in the areas under the receiver operating characteristic curve (AUCs) for predicting mutations (AUC, 0.76, 0.82, 0.78, 0.81, and 0.82, respectively; > .05) and codeletion in gliomas with mutations (AUC, 0.83, 0.81, 0.82, 0.83, and 0.88, respectively; > .05). A regression model with an value of 0.84 was used for the Ki-67 labeling index and histogram features of the diffusion metrics. Conclusion Whole-tumor histogram analysis of multiple diffusion metrics is a promising approach for glioma isocitrate dehydrogenase and genotyping, and the performance of diffusion-tensor imaging is similar to that of advanced diffusion models. Clinical trial registration no. ChiCTR2100048119 © RSNA, 2021
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1148/radiol.210820DOI Listing
March 2022

Investigation of Anxiety, Depression, Sleep, and Family Function in Caregivers of Children With Epilepsy.

Front Neurol 2021 25;12:744017. Epub 2021 Oct 25.

Department of Neurology, Shenzhen Children's Hospital, Shenzhen, China.

Epilepsy is a chronic disease that places a heavy burden on caregivers. Previous studies have shown that caregivers of epilepsy patients often experience anxiety and depression; however, few comprehensive studies have assessed their sleep quality and family function. Based on the current understanding of the anxiety and depression state of caregivers in children with epilepsy, we further explored the caregivers' sleep and family function and evaluated the predictors of the depression state of caregivers. In this cross-sectional online anonymous survey, we sent an online questionnaire to the caregivers of children with epilepsy who visited our hospital. The QR code of the questionnaire was scanned at the follow-up course to conduct an online survey. The questionnaire contained questions about sociodemographic and clinical information, the Self-rating Anxiety Scale, Self-rating Depression Scale, Pittsburgh Sleep Quality Index, and the Family Assessment Device. A total of 308 caregivers of children with epilepsy aged 0-12 years were included in this study. The mean age of children with epilepsy was 4.8 ± 3.18 years, and the average illness duration was 34.2 ± 29.18 months. Further, 47.1% of the children took three or more anti-seizure medications, and 43.2% were on ketogenic diet therapy. We found that in 77.9% of the cases, the subjects were the mothers, in 89% there was more than one co-caregiver, and in 51.9%, financial help was required. Further, 63.6% of the caregivers thought they could not get enough access to disease knowledge education, and 83.7% perceived epilepsy was a terrible disease. Our results also showed that 65.6% of the caregivers were in depression status, 41.9% were in anxiety status, and 49.0% had poor sleep quality. The proportion of unhealthy family functioning in each subscale was 45.1-96.1%, and the unhealthy behavior control function accounted for 96.1%. Binary logistic regression analysis of the data showed that without co-caregivers [odds ratio (OR), 5.193], free of anxiety status (OR, 0.063), good sleep quality (OR, 0.446), healthy family role dimension (OR, 0.344), and healthy family general functional dimension (OR, 0.259) were predictors of depression status in caregivers of children with epilepsy. Anxiety and depression status are common in caregivers of children with epilepsy, with depression status being more prominent. Moreover, a considerable proportion of caregivers had poor sleep quality and unhealthy family function. The caregivers' anxiety status, sleep quality, family role dimension, family general function dimension, and the number of co-caregivers were predictors of depression status in caregivers. In clinical practice, caregivers' anxiety and depression status, poor sleep quality, and unhealthy family functioning should be addressed along with the treatment of children with epilepsy.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.3389/fneur.2021.744017DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8575681PMC
October 2021

Bulk Electrocatalytic NADH Cofactor Regeneration with Bipolar Electrochemistry.

Angew Chem Int Ed Engl 2022 01 23;61(3):e202111804. Epub 2021 Nov 23.

Engineering Research Center for Nanomaterials, Henan University, Kaifeng, China.

Electrochemical regeneration of reduced nicotinamide adenine dinucleotide (NADH) is an extremely important challenge for the electroenzymatic synthesis of many valuable chemicals. Although some important progress has been made with modified electrodes concerning the reduction of NAD , the scale-up is difficult due to mass transport limitations inherent to large-size electrodes. Here, we propose instead to employ a dispersion of electrocatalytically active modified microparticles in the bulk of a bipolar electrochemical cell. In this way, redox reactions occur simultaneously on all of these individual microelectrodes without the need of a direct electrical connection. The concept is validated by using [Rh(Cp*)(bpy)Cl] functionalized surfaces, either of carbon felt as a reference material, or carbon microbeads acting as bipolar objects. In the latter case, enzymatically active 1,4-NADH is electroregenerated at the negatively polarized face of the particles. The efficiency of the system can be fine-tuned by controlling the electric field in the reaction compartment and the number of dispersed microelectrodes. This wireless bioelectrocatalytic approach opens up very interesting perspectives for electroenzymatic synthesis in the bulk phase.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1002/anie.202111804DOI Listing
January 2022

A man with sensation of cold water running down the face.

J Neurol 2022 Mar 24;269(3):1691-1694. Epub 2021 Sep 24.

Department of Acupuncture and Rehabilitation, Affiliated Hospital of Nanjing University of Chinese Medicine, Nanjing, 210029, Jiangsu, China.

View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1007/s00415-021-10815-zDOI Listing
March 2022

Mogroside V Alleviates Oocyte Meiotic Defects and Quality Deterioration in Benzo(a)pyrene-Exposed Mice.

Front Pharmacol 2021 26;12:722779. Epub 2021 Aug 26.

State Key Laboratory for Conservation and Utilization of Subtropical Agro-bioresources, College of Animal Science and Technology, Guangxi University, Nanning, China.

Accumulating evidence has demonstrated that benzo(a)pyrene (BaP) exposure adversely affects female reproduction, especially oocyte meiotic maturation and subsequent embryo development. Although we previously found that mogroside V (MV), a major bioactive component of , can protect oocytes from quality deterioration caused by certain stresses, whether MV can alleviate BaP exposure-mediated oocyte meiotic defects remains unknown. In this study, female mice were exposed to BaP and treated concomitantly with MV by gavage. We found that BaP exposure reduced the oocyte maturation rate and blastocyst formation rate, which was associated with increased abnormalities in spindle formation and chromosome alignment, reduced acetylated tubulin levels, damaged actin polymerization and reduced Juno levels, indicating that BaP exposure results in oocyte nucleic and cytoplasmic damage. Interestingly, MV treatment significantly alleviated all the BaP exposure-mediated defects mentioned above, indicating that MV can protect oocytes from BaP exposure-mediated nucleic and cytoplasmic damage. Additionally, BaP exposure increased intracellular ROS levels, meanwhile induced DNA damage and early apoptosis in oocytes, but MV treatment ameliorated these defective parameters, therefore it is possible that MV restored BaP-mediated oocyte defects by reducing oxidative stress. In summary, our findings demonstrate that MV might alleviate oocyte meiotic defects and quality deterioration in BaP-exposed mice.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.3389/fphar.2021.722779DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8428525PMC
August 2021

Multisequence Quantitative Magnetic Resonance Neurography of Brachial and Lumbosacral Plexus in Chronic Inflammatory Demyelinating Polyneuropathy.

Front Neurosci 2021 23;15:649071. Epub 2021 Jul 23.

Department of Radiology, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China.

Background And Purpose: Chronic inflammatory demyelinating polyneuropathy (CIDP) is an uncommon demyelinating disorder. Although treatable, it is difficult to diagnose. The purpose of this study was to evaluate the diagnostic performance and abnormalities of plexus via quantitative multisequence magnetic resonance neurography (MRN) for CIDP.

Methods: Brachial and lumbosacral (LS) plexus of 37 CIDP patients and 37 age- and gender-matched controls were examined by using multisequence MRN. Nerve diameter, nerve-to-muscle T2 signal intensity ratio (nT2), contrast-enhanced ratio (CR), fractional anisotropy (FA), and apparent diffusion coefficient (ADC) were determined in both plexus, and tractographies were performed. The disease histories and the Inflammatory Rasch-built Overall Disability Scale (I-RODS) questionnaire scores were documented before MRI scans.

Results: The sizes of nerve roots were larger in CIDP ( < 0.01). CR, nT2, and ADC were significantly higher, while FA was lower in CIDP than in controls ( < 0.01). FA had the highest sensitivity (0.809) and area under the curve (AUC) (0.925), while the highest specificity was 0.961 for CR in single parameters. The combination of FA and CR has the highest sensitivity, specificity, accuracy, and AUC in the LS plexus. CR only had a weak correlation with nT2 ( < 0.05). ADC and diameter had a positive correlation with nT2, and the diameter and nT2 had a negative correlation with FA in CIDP ( < 0.05). FA had a negative correlation with the duration in the CIDP ('s = -0.404, < 0.05). There was no significant correlation between the I-RODS scores and MR multiparameters ( < 0.05).

Conclusion: Multisequence MRN possesses a high diagnostic performance in the LS plexus. Sampling perfection with application-optimized contrasts using different flip angle evolutions (SPACE) combined with DTI and contrast enhancement serves as a recommended composite protocol for CIDP.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.3389/fnins.2021.649071DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8346234PMC
July 2021

Upregulation of Stress-Induced Protein Kinase CK1 Delta is associated with a Poor Prognosis for patients with Hepatocellular Carcinoma.

Genet Test Mol Biomarkers 2021 Jul;25(7):504-514

Department of Gastrointestinal Surgery, The First Affiliated Hospital of Jinan University, Guangzhou, China.

This study was designed to analyze the expression of CSNK1D in hepatocellular carcinoma (HCC) and investigate the relationship between the expression of CSNK1D and the prognosis of HCC patients. The CSNK1D and alpha-fetoprotein (AFP) expression levels in patients with HCC and their corresponding clinical data were downloaded from The Cancer Genome Atlas (TCGA) and sorted with a Perl program. CSNK1D and AFP expression differences in liver tissue and liver cancer were compared and analyzed, based on the online database human cancer metastasis database, the relationships between the expression levels of CSNK1D and AFP and the proliferation and metastases of HCC were explored. The immunohistochemical data obtained from the Human Protein Atlas Database further verified the differences in the expression levels of CSNK1D and AFP in liver tissues and liver cancer tissues. Through Kaplan-Meier survival analysis, the effects of CSNK1D and AFP expression levels on the prognosis of patients with HCC were investigated, and the influences of and patients' gender, age and grades of cancer cells, tumor size, the status of lymph node metastasis, distant metastasis, and tumor stage on the expression of CSNK1D were analyzed with R language. The influence of differential expressions of CSNK1D on survival time was compared and the prognostic factors influencing the survival of HCC patients were statistically explored by univariate analysis and multivariate analysis. The potential influencing mechanism of CSNK1D on the prognosis of HCC patients was explored by Gene Set Enrichment Analysis (GSEA) enrichment. The expression level of CSNK1D and AFP in cancer foci was significantly higher than that in normal tissues, However, in the same patient, the expression levels of AFP in paracarcinoma tissues and cancer tissues showed no significant difference. The expression level of CSNK1D in HCC with distant metastases was higher than that in those without metastasis, but the expression level of AFP in metastatic HCC was lower than that in those HCC without metastases. In immunohistochemical tests, CSNK1D was moderately positive in normal liver tissues, slightly positive in normal bile duct tissues, and highly positive in HCC. AFP was slightly positive in normal liver tissues and negative in HCC, but it was not detected in normal intrahepatic bile duct tissue. Survival analysis results suggested that the higher expression level of CSNK1D corresponded to the shorter survival period, whereas the expression level of AFP showed no significant influence on survival time. The expression level of CSNK1D was not correlated with gender, age, the status of lymph node metastasis status, or distant metastasis of patients. The main factors influencing the expression level of CSNK1D included tumor size, cancer cell grade, and tumor stage. The expression levels of CSNK1D in T2 and T3 were higher than that in T1. The expression levels of CSNK1D in G3 and G4 were higher than that in G1. The expression levels of CSNK1D in Stage II and Stage III were higher than that in Stage I. Univariate analysis suggested that tumor size, cell grade, distant metastasis, clinical stage, and CSNK1D expression level were the prognostic factors influencing the survival of patients. Multivariate analysis suggested that CSNK1D expression level was an independent factor influencing the prognosis of HCC patients. GSEA enrichment analysis indicated that CSNK1D mainly affected the prognosis of HCC patients through cell cycle, WNT signaling pathway, amino acid degradation metabolism, and other pathways. CSNK1D is an independent influencing factor for the prognosis of HCC patients and has the potential to be developed as a potential therapeutic target for HCC, and better than AFP in predicting the prognosis of HCC.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1089/gtmb.2020.0093DOI Listing
July 2021

Quantification of bone marrow edema in rheumatoid arthritis by using high-speed T2-corrected multiecho acquisition of H magnetic resonance spectroscopy: a feasibility study.

Clin Rheumatol 2021 Nov 21;40(11):4639-4647. Epub 2021 Jun 21.

Department of Radiology, The First Affiliated Hospital of Guangxi Medical University, No. 6 Shuangyong Road, Nanning, Guangxi, 530021, People's Republic of China.

Objective: To determine whether high-speed T2-corrected multiecho (HISTO) sequences can quantify bone marrow edema (BME) in the capitate bone in rheumatoid arthritis (RA), and whether the HISTO fat fraction (FF) reflects therapeutic effectiveness.

Methods: In this prospective study, 25 RA patients (19 women; average age, 45.08 ± 13.48 years) underwent 3.0-T MRI with HISTO at the baseline and after 4, 8, and 12 weeks of treatment. Rheumatoid factor (RF), erythrocyte sedimentation rate (ESR), C-reactive protein (CRP), platelet (PLT) count, and 28-joint Disease Activity Score using ESR (DAS28-ESR) were recorded on the day of each MRI examination by a rheumatologist blinded to the MRI findings. In addition, 21 healthy subjects (15 women; age, 49.17 ± 6.56 years) underwent only the HISTO sequence at a single time point.

Results: HISTO FF values were significantly higher in the control group (74.5% ± 3.1%; range, 68.6-79.3%) than in the patient group (55.8% ± 17.7%; range, 15.6-79.0%) at the baseline (independent-samples t-test: t = 5.257, P = 0.000). The changes in HISTO FF and DAS28-ESR showed moderate negative correlations with each other at 4, 8, and 12 weeks, and all of them were statistically significant (P < 0.05). As the HISTO FF increased, the DAS28-ESR decreased.

Conclusion: The HISTO sequence can measure the bone marrow FF of the wrist joint bones in RA patients. The HISTO FF value increased as the DAS28-ESR decreased. The HISTO sequence may help quantify BME in RA and help monitor the effectiveness of RA treatment. Key Points •The HISTO sequence could measure the bone marrow FF of the wrist joint bones. •FF value increased as the DAS28-ESR decreased in RA patients. •The HISTO sequence can monitor the therapeutic effect of RA.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1007/s10067-021-05764-xDOI Listing
November 2021

Application value of CAIPIRINHA-VIBE with MOCO in liver magnetic resonance examination.

Eur J Radiol 2021 Jul 28;140:109739. Epub 2021 Apr 28.

Department of Radiology, The Second Xiangya Hospital, Central South University, No.139 Middle Renmin Road, Changsha, Hunan, 410011, China. Electronic address:

Objective: To compare the image quality of VIBE sequence using controlled aliasing in parallel imaging results in higher acceleration (CAIPIRINHA-VIBE) and using generalized autocalibrating partially parallel acquisitions (GRAPPA-VIBE) in liver magnetic resonance examination, and to evaluate the effect of non-rigid 3D-registration motion correction (MOCO) combined with CAIPIRINHA-VIBE on liver spatial location registration.

Methods: A total of 85 patients underwent pre-contrast GRAPPA-VIBE and CAIPIRINHA-VIBE breath-hold scan in the mask phase, and then underwent CAIPIRINHA-VIBE breath-hold scan in arterial phase, portal vein phase and delay phase after administration. After the scanning of four phases of CAIPIRINHA-VIBE completed, 3D images without and with MOCO of each phase were automatically generated. The images quality of GRAPPA-VIBE and CAIPIRINHA-VIBE without MOCO in the mask phase was scored subjectively by two physicians. The number of slices at the top of the diaphragm in the arterial phase was taken as the base slice, and that in the other stages subtracted with the base slice for CAIPIRINHA without and with MOCO. The range of diaphragm movement in each phase was counted by + N/- N statistics.

Results: The image quality and the scores of CAIPIRINHA-VIBE were significantly higher than those of GRAPPA-VIBE in respiratory motion artifact suppression, liver edge sharpness and intrahepatic vascular sharpness (p < 0.05). The spatial position consistency of the liver with MOCO is significantly better than that without MOCO.

Conclusion: CAIPIRINHA-VIBE with MOCO can be used instead of conventional GRAPPA-VIBE sequence in upper abdominal MRI enhancement examination, especially for patients with poor breath-hold.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.ejrad.2021.109739DOI Listing
July 2021

Erratum: The Role of CHK1 Varies with the Status of Oestrogen-receptor and Progesterone-receptor in the Targeted Therapy for Breast Cancer : Erratum.

Int J Biol Sci 2021 20;17(3):911-912. Epub 2021 Feb 20.

Department of Pathology, School of Basic Medical Sciences, Shandong University, Jinan, 250012, China.

[This corrects the article DOI: 10.7150/ijbs.41627.].
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.7150/ijbs.58372DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7975706PMC
February 2021

Mogroside V Protects Porcine Oocytes From Lipopolysaccharide-Induced Meiotic Defects.

Front Cell Dev Biol 2021 2;9:639691. Epub 2021 Mar 2.

State Key Laboratory for Conservation and Utilization of Subtropical Agro-bioresources, College of Animal Science and Technology, Guangxi University, Nanning, China.

Accumulating evidence has demonstrated that lipopolysaccharide (LPS) compromises female reproduction, especially oocyte maturation and competence. However, methods to protect oocyte quality from LPS-induced deterioration remain largely unexplored. We previously found that mogroside V (MV) can promote oocyte maturation and embryonic development. However, whether MV can alleviate the adverse effects of LPS exposure on oocyte maturation is unclear. Thus, in this study, we used porcine oocytes as a model to explore the effects of MV administration on LPS-induced oocyte meiotic defects. Our findings show that supplementation with MV protected oocytes from the LPS-mediated reduction in the meiotic maturation rate and the subsequent blastocyst formation rate. In addition, MV alleviated the abnormalities in spindle formation and chromosome alignment, decrease in α-tubulin acetylation levels, the disruption of actin polymerization, and the reductions in mitochondrial contents and lipid droplet contents caused by LPS exposure. Meanwhile, LPS reduced mA levels in oocytes, but MV restored these epigenetic modifications. Furthermore, MV reduced reactive oxygen species (ROS) levels and early apoptosis in oocytes exposed to LPS. In summary, our study demonstrates that MV can protect oocytes from LPS-induced meiotic defects in part by reducing oxidative stress and maintaining mA levels.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.3389/fcell.2021.639691DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7982822PMC
March 2021
-->