Publications by authors named "Huiqin An"

3 Publications

  • Page 1 of 1

Design and cytocompatibility of chitosan-based thermoresponsive cell culture plates.

J Appl Biomater Funct Mater 2016 Nov 2;14(4):e404-e412. Epub 2016 Nov 2.

Tianjin Key Laboratory of Fiber Modification and Functional Fiber, Tianjin Polytechnic University, Tianjin - PR China.

Background: The aim of this study was to develop a novel thermoresponsive material suited for tissue engineering and investigate the growth and harmless detachment of cells cultured on the surface of thermoresponsive tissue culture polystyrene (TCPS).

Methods: Thermoresponsive N-isopropylacrylamide (NIPAAm) and biocompatible chitosan (CS) were grafted onto the surface of TCPS by ultraviolet (UV)-induced graft polymerization. The chemical composition, surface morphology and thermoresponsiveness of the modified TCPS were investigated by X-ray photoelectron spectroscopy (XPS), atom force microscopy (AFM) and contact angle (CA), respectively. Furthermore, the growth and detachment behaviors of mouse fibroblast cells (L929) on the surface of the modified TCPS were studied by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay.

Results: The modified TCPS exhibited good hydrophobic/hydrophilic property alterations in response to temperature. The cytocompatibility of the materials was improved due to the introduction of CS. Cells could be spontaneously detached from the surface without any damage, by controlling environmental temperature. The viability of cells obtained by temperature induction was higher than that obtained by enzymatic digestion.

Conclusions: This study developed a simple and economical method to fabricate thermoresponsive cell culture dishes and provided new thoughts and experimental bases for exploring novel material applied in tissue engineering.
View Article and Find Full Text PDF

Download full-text PDF

Source Listing
November 2016

[Analysis of personality characteristics of sudden deafness patients].

Lin Chung Er Bi Yan Hou Tou Jing Wai Ke Za Zhi 2014 Apr;28(7):484-6

Objective: To Study the personality characteristics in patients with sudden deafness.

Method: Thirty-eight sudden deafness patients and 45 healthy volunteers were assessed by Eysenck personality questionnaire (EPQ).

Result: The standard scores of P, E, N dimensions of patients with sudden deafness were greater than healthy volunteers. The standard score in L dimensions of patients with sudden deafness was less than healthy volunteers. The difference of the standard score of P dimension revealed statistical significance (P < 0.05). The difference of the standard score of N dimension revealed statistical significance (P < 0.01). The difference of the standard scores of E, L dimensions revealed no statistical significance (P > 0.05).

Conclusion: Sudden deafness patients have emotional instability and psychoticism personality characteristics.
View Article and Find Full Text PDF

Download full-text PDF

April 2014

[One case of laryngeal pleomorphic adenoma].

Lin Chung Er Bi Yan Hou Tou Jing Wai Ke Za Zhi 2013 May;27(9):492-3

A male patient, 55 years old, suffered from intermittent sound,voice depression and shortness of breath for one year,and from dysphagia for 3-4 months. Through fiber laryngoscopy,we could see tumor in the left posterior aryepiglottic fold. The tumor's surface was smooth. A portion of the tumor protruded to the laryngeal cavity and the aryepiglottic fold external,it also covered most of the glottis. Bilateral vocal cord were smooth and had good mobility. Throat CT demonstrated an irregular soft tissue mass on the left side of the aryepiglottic fold in supraglottic area with obscure normal boundary from adjacent structure. The left side of pyriform sinus became shallow without obvious bone destruction. The pathological report showed pleomorphic adenoma. The diagnosis was laryngeal pleomorphic adenoma.
View Article and Find Full Text PDF

Download full-text PDF

May 2013