Publications by authors named "Huimin Zhang"

508 Publications

Dynamics of Post-Translational Modification Inspires Drug Design in the Kinase Family.

J Med Chem 2021 Oct 20. Epub 2021 Oct 20.

Center for Systems Biology, Department of Bioinformatics, School of Biology and Basic Medical Sciences, Soochow University, Suzhou 215123, China.

Post-translational modification (PTM) on protein plays important roles in the regulation of cellular function and disease pathogenesis. The systematic analysis of PTM dynamics presents great opportunities to enlarge the target space by PTM allosteric regulation. Here, we presented a framework by integrating the sequence, structural topology, and particular dynamics features to characterize the functional context and druggabilities of PTMs in the well-known kinase family. The machine learning models with these biophysical features could successfully predict PTMs. On the other hand, PTMs were identified to be significantly enriched in the reported allosteric pockets and the allosteric potential of PTM pockets were thus proposed through these biophysical features. In the end, the covalent inhibitor DC-Srci-6668 targeting the PTM pocket in c-Src kinase was identified, which inhibited the phosphorylation and locked c-Src in the inactive state. Our findings represent a crucial step toward PTM-inspired drug design in the kinase family.
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http://dx.doi.org/10.1021/acs.jmedchem.1c01076DOI Listing
October 2021

Association of remnant cholesterol with nonalcoholic fatty liver disease: a general population-based study.

Lipids Health Dis 2021 Oct 17;20(1):139. Epub 2021 Oct 17.

Department of Cardiology, Jiangxi Provincial People's Hospital Affiliated to Nanchang University, Nanchang, People's Republic of China, 330006.

Background: Remnant cholesterol (RC) mediates the progression of coronary artery disease, diabetic complications, hypertension, and chronic kidney disease. Limited information is available on the association of RC with nonalcoholic fatty liver disease (NAFLD). This study aimed to explore whether RC can be used to independently evaluate the risk of NAFLD in the general population and to analyze the predictive value of RC for NAFLD.

Methods: The study included 14,251 subjects enrolled in a health screening program. NAFLD was diagnosed by ultrasound, and the association of RC with NAFLD was assessed using the receiver operating characteristic (ROC) curve and logistic regression equation.

Results: Subjects with elevated RC had a significantly higher risk of developing NAFLD after fully adjusting for potential confounding factors (OR 1.77 per SD increase, 95% CI 1.64-1.91, P trend< 0.001). There were significant differences in this association among sex, BMI and age stratification. Compared with men, women were facing a higher risk of RC-related NAFLD. Compared with people with normal BMI, overweight and obesity, the risk of RC-related NAFLD was higher in thin people. In different age stratifications, when RC increased, young people had a higher risk of developing NAFLD than other age groups. Additionally, ROC analysis results showed that among all lipid parameters, the AUC of RC was the largest (women: 0.81; men: 0.74), and the best threshold for predicting NAFLD was 0.54 in women and 0.63 in men.

Conclusions: The results obtained from this study indicate that (1) in the general population, RC is independently associated with NAFLD but not with other risk factors. (2) Compared with traditional lipid parameters, RC has a better predictive ability for NAFLD in men.
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http://dx.doi.org/10.1186/s12944-021-01573-yDOI Listing
October 2021

An Overview of Micronanoswarms for Biomedical Applications.

ACS Nano 2021 Oct 14. Epub 2021 Oct 14.

School of Science and Engineering, The Chinese University of Hong Kong, Shenzhen 518172, China.

Micronanoswarms have attracted extensive attention worldwide due to their great promise in biomedical applications. The collective behaviors among thousands, or even millions, of tiny active agents indicate immense potential for benefiting the progress of clinical therapeutic and diagnostic methods. In recent years, with the development of smart materials, remote actuation modalities, and automatic control strategies, the motion dexterity, environmental adaptability, and functionality versatility of micronanoswarms are improved. Swarms can thus be designed as dexterous platforms inside living bodies to perform a multitude of tasks related to healthcare. Existing surveys summarize the design, functionalization, and biomedical applications of micronanorobots and the actuation and motion control strategies of micronanoswarms. This review presents the recent progress of micronanoswarms, aiming for biomedical applications. The recent advances on structural design of artificial, living, and hybrid micronanoswarms are summarized, and the biomedical applications that could be tackled using micronanoswarms are introduced, such as targeted drug delivery, hyperthermia, imaging and sensing, and thrombolysis. Moreover, potential challenges and promising trends of future developments are discussed. It is envisioned that the future success of these promising tools will have a significant impact on clinical treatment.
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http://dx.doi.org/10.1021/acsnano.1c07363DOI Listing
October 2021

Effect of Postmortem Phases on Lamb Meat Quality: A Physicochemical, Microstructural and Water Mobility Approach.

Food Sci Anim Resour 2021 Sep 1;41(5):802-815. Epub 2021 Sep 1.

Institute of Food Science and Technology, Chinese Academy of Agricultural Sciences, Key Laboratory of Agro-Products Quality and Safety Control in Storage and Transport Process, Ministry of Agriculture and Rural Affairs, Beijing 100193, China.

To investigate the effect of postmortem phases on lamb meat quality, the physicochemical quality, microstructure and water mobility of oyster cut, short loin, knuckle and silverside muscles from Small-Tail Han sheep were evaluated in the pre-rigor, rigor mortis and post-rigor phases. Pre-rigor lamb meat had higher pH and water holding capacity (WHC), whereas lower CIE L*, b*, hue angle values than rigor mortis and post-rigor meat (p<0.05). The Warner-Bratzler shear force (WBSF) values were higher in rigor mortis short loin and silverside than their pre-rigor and post-rigor counterparts, pre-rigor short loin had lower WBSF value than its post-rigor counterpart (p<0.05). Muscle fibers shrank laterally and longitudinally during the onset of rigor mortis. Rigor mortis and post-rigor lamb meat exhibited wide I-bands, dark A-bands, short sarcomeres and large inter-myofibrillar spaces. The shift of immobilized water to free water and repulsion from the intra-myofibrillar space to the extracellular space result in the increase of water loss in rigor mortis and post-rigor lamb meat. The results of the principal component analysis (PCA) indicated that rigor mortis and post-rigor lamb meat had similar quality properties but different from pre-rigor lamb meat. In conclusion, the lamb meat in the pre-rigor phase had good tenderness, color and WHC. The results of this research could provide some theoretical references for lamb meat production and processing.
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http://dx.doi.org/10.5851/kosfa.2021.e37DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8460324PMC
September 2021

Low-density-lipoprotein-receptor-related protein 1 mediates Notch pathway activation.

Dev Cell 2021 Oct 1. Epub 2021 Oct 1.

Key Laboratory of Structural Biology of Zhejiang Province, School of Life Sciences, Westlake University, Hangzhou 310024, Zhejiang, China; Westlake Laboratory of Life Sciences and Biomedicine, Hangzhou 310024, Zhejiang, China; Institute of Biology, Westlake Institute for Advanced Study, Hangzhou 310024, Zhejiang, China. Electronic address:

The Notch signaling pathway controls cell growth, differentiation, and fate decisions, and its dysregulation has been linked to various human genetic disorders and cancers. To comprehensively understand the global organization of the Notch pathway and identify potential drug targets for Notch-related diseases, we established a protein interaction landscape for the human Notch pathway. By combining and analyzing genetic and phenotypic data with bioinformatics analysis, we greatly expanded this pathway and identified many key regulators, including low-density-lipoprotein-receptor-related protein 1 (LRP1). We demonstrated that LRP1 mediates the ubiquitination chain linkage switching of Delta ligands, which further affects ligand recycling, membrane localization, and stability. LRP1 inhibition led to Notch signaling inhibition and decreased tumorigenesis in leukemia models. Our study provides a glimpse into the Notch pathway interaction network and uncovers LRP1 as one critical regulator of the Notch pathway, as well as a possible therapeutic target for Notch-related cancers.
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http://dx.doi.org/10.1016/j.devcel.2021.09.015DOI Listing
October 2021

Application of the CRISPR/Cas9-based gene editing technique in basic research, diagnosis, and therapy of cancer.

Mol Cancer 2021 Oct 1;20(1):126. Epub 2021 Oct 1.

Shanxi Key Laboratory of Otorhinolaryngology Head and Neck Cancer, Shanxi Province Clinical Medical Research Center for Precision Medicine of Head and Neck Cancer, Department of Otolaryngology Head & Neck Surgery, First Hospital of Shanxi Medical University, Taiyuan, 030001, Shanxi, China.

The 2020 Nobel Prize in Chemistry was awarded to Emmanuelle Charpentier and Jennifer Doudna for the development of the Clustered regularly interspaced short palindromic repeats/CRISPR-associated nuclease9 (CRISPR/Cas9) gene editing technology that provided new tools for precise gene editing. It is possible to target any genomic locus virtually using only a complex nuclease protein with short RNA as a site-specific endonuclease. Since cancer is caused by genomic changes in tumor cells, CRISPR/Cas9 can be used in the field of cancer research to edit genomes for exploration of the mechanisms of tumorigenesis and development. In recent years, the CRISPR/Cas9 system has been increasingly used in cancer research and treatment and remarkable results have been achieved. In this review, we introduced the mechanism and development of the CRISPR/Cas9-based gene editing system. Furthermore, we summarized current applications of this technique for basic research, diagnosis and therapy of cancer. Moreover, the potential applications of CRISPR/Cas9 in new emerging hotspots of oncology research were discussed, and the challenges and future directions were highlighted.
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http://dx.doi.org/10.1186/s12943-021-01431-6DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8484294PMC
October 2021

Phosphate enhanced uranium stable immobilization on biochar supported nano zero valent iron.

J Hazard Mater 2021 Sep 11;424(Pt A):127119. Epub 2021 Sep 11.

Guangdong Provincial Key Laboratory of Radionuclides Pollution Control and Resources, School of Environmental Science and Engineering, Guangzhou University, Guangzhou 510006, China; Guangdong Key Laboratory of Environmental Catalysis and Health Risk Control, School of Environmental Science and Engineering, Institute of Environmental Health and Pollution Control, Guangdong University of Technology, Guangzhou 510006, China. Electronic address:

Uranium (U) immobilization from wastewater by zero valent iron (ZVI) was widely concerned through reduction and surface adsorption. Releasing of U due to re-oxidation of U(IV) into U(VI) limited the application of ZVI in U decontamination. In this work, a kind of biochar supported nano zero valent iron (Fe/BC(900)) was obtained by carbothermal reduction of starch mixed with ferric nitrate at 900 °C. U immobilization behavior by Fe/BC(900) in the presence of phosphate (P) was investigated. The U immobilization reaction was adjusted by controlling the sequence of U, Fe/BC(900) and P. U immobilization efficiency was enhanced to 99.9% in the presence of P. Reaction sequence of U, Fe/BC(900) and P influenced the U immobilization efficiency, which followed the order of (U-P)+Fe/BC(900)>(U- Fe/BC(900))+P>U+Fe/BC(900)>(P-Fe/BC(900))+U. P and nZVI both contributed to enhancing U immobilization through precipitation of uranyl-P and reductive co-precipitate (U(IV)) in a wide pH range. The released Fe ions could precipitate with uranyl and phosphate. Consumption of P and nZVI in the (P-Fe/BC(900))+U system limited U immobilization ability. The precipitate is highly dependent on U, P and Fe elements. U desorption in (U-P)+Fe/BC(900) system was not observed with stability.
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http://dx.doi.org/10.1016/j.jhazmat.2021.127119DOI Listing
September 2021

A cross-sectional study of implicit rationing of care in publicly-funded nursing homes in Shanghai, China.

J Nurs Manag 2021 Sep 30. Epub 2021 Sep 30.

School of International and Public Affairs, Shanghai Jiao Tong University, China.

Aims: To investigate the level of implicit care rationing and its association with training needs in nursing homes in Shanghai, China.

Background: Nursing homes in Shanghai are confronted with a lack of care resources. Implicit care rationing can emerge due to inadequate training of care workers.

Methods: A cross-sectional survey was conducted between September 10 and November 17, 2020. A total of 374 care workers from 16 randomly selected nursing homes from each of the administrative districts participated the surveys. The Basel Extent of Rationing of Nursing Care-Nursing Home instrument and the Training Needs Analysis were adopted to measure implicit care rationing and training needs, respectively. Multiple regression techniques were used to explore the factors associated with implicit care rationing.

Results: Activities related to social care, documentation, and activation/rehabilitation (mean rating = 2.8, 1.89, and 1.93 respectively) were mostly likely to be rationed. Training needs of ADL, activation/rehabilitation, and documentation were significantly related to their implicit rationing (β = 0.864, 0.21, and 0.166, respectively, p<0.01).

Conclusion: Training needs are crucial determinants of implicit care rationing.

Implications For Nursing Management: Quality control systems are needed to ensure care comprehensiveness. The current training system should be re-designed according to results of training needs analysis.
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http://dx.doi.org/10.1111/jonm.13479DOI Listing
September 2021

Cyanidin-3-O-glucoside and its metabolite protocatechuic acid ameliorate 2-amino-1-methyl-6-phenylimidazo[4,5-b]pyridine (PhIP) induced cytotoxicity in HepG2 cells by regulating apoptotic and Nrf2/p62 pathways.

Food Chem Toxicol 2021 Sep 25;157:112582. Epub 2021 Sep 25.

Beijing Advanced Innovation Center for Food Nutrition and Human Health, Beijing Engineering and Technology Research Center of Food Additives, Beijing Technology and Business University, Beijing, 100048, China.

The present study investigated the protective effects and mechanism of action of cyanidin-3-O-glucoside (C3G) and its major metabolite protocatechuic acid (PCA) against 2-amino-1-methyl-6-phenylimidazo[4,5-b]pyridine (PhIP) induced cytotoxicity in HepG2 cells. The results demonstrated that C3G and PCA dose-dependently suppressed PhIP-induced mutation in Salmonella typhimurium TA98, and inhibited PhIP-induced cytotoxicity and apoptosis in HepG2 cells. Western blot analysis indicated that C3G and PCA minimized PhIP-induced cell damage by reversing the abnormal expression of Bax/Bcl-2, Cytochrome c, cleaved Caspase-3, XIAP, Nrf2, HO-1, LC3 and p62 involved in intrinsic apoptotic and Nrf2/p62 pathways. Molecular docking results revealed that C3G and PCA were able to interfere with Nrf2 signaling and apoptotic cascade through binding to Keap1 and Bcl-2. Moreover, the protective effect of C3G was stronger than that of PCA. These findings suggested that dietary consumption of food sources rich in C3G can fight against the health risks of heterocyclic aromatic amines.
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http://dx.doi.org/10.1016/j.fct.2021.112582DOI Listing
September 2021

Suppressing chlorophyll degradation by silencing OsNYC3 improves rice resistance to Rhizoctonia solani, the causal agent of sheath blight.

Plant Biotechnol J 2021 Sep 28. Epub 2021 Sep 28.

Key Laboratory of Plant Functional Genomics of The Ministry of Education, Jiangsu Key Laboratory of Crop Genomics and Molecular Breeding, Agricultural College of Yangzhou University, Yangzhou, China.

Necrotrophic fungus Rhizoctonia solani Kühn (R. solani) causes serious diseases in many crops worldwide, including rice and maize sheath blight (ShB). Crop resistance to the fungus is a quantitative trait and resistance mechanism remains largely unknown, severely hindering the progress on developing resistant varieties. In this study, we found that resistant variety YSBR1 has apparently stronger ability to suppress the expansion of R. solani than susceptible Lemont in both field and growth chamber conditions. Comparison of transcriptomic profiles shows that the photosynthetic system including chlorophyll biosynthesis is highly suppressed by R. solani in Lemont but weakly in YSBR1. YSBR1 shows higher chlorophyll content than that of Lemont, and inducing chlorophyll degradation by dark treatment significantly reduces its resistance. Furthermore, three rice mutants and one maize mutant that carry impaired chlorophyll biosynthesis all display enhanced susceptibility to R. solani. Overexpression of OsNYC3, a chlorophyll degradation gene apparently induced expression by R. solani infection, significantly enhanced ShB susceptibility in a high-yield ShB-susceptible variety '9522'. However, silencing its transcription apparently improves ShB resistance without compromising agronomic traits or yield in field tests. Interestingly, altering chlorophyll content does not affect rice resistance to blight and blast diseases, caused by biotrophic and hemi-biotrophic pathogens, respectively. Our study reveals that chlorophyll plays an important role in ShB resistance and suppressing chlorophyll degradation induced by R. solani infection apparently improves rice ShB resistance. This discovery provides a novel target for developing resistant crop to necrotrophic fungus R. solani.
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http://dx.doi.org/10.1111/pbi.13715DOI Listing
September 2021

Research on Effectiveness of Prior Cancer on Survival Outcomes for Patients with Nonmetastatic Triple-Negative Breast Cancer: A Competing Risk Analysis and Propensity Score Matching Analysis of the SEER Database.

J Oncol 2021 17;2021:9988624. Epub 2021 Sep 17.

Department of Breast Surgery, The First Affiliated Hospital of Xi'an Jiaotong University, Xi'an, China.

Introduction: Knowledge of the effect of prior cancer on long-term survival outcomes for patients with nonmetastatic triple-negative breast cancer (TNBC) remained unclear. The aim of this study was to explore and identify the effectiveness of prior cancer on breast cancer-specific death (BCSD) and other cause-specific death (OCSD) in patients with nonmetastatic TNBC.

Materials And Methods: Data of 29,594 participants with nonmetastatic TNBC patients were extracted from the Surveillance, Epidemiology, and End Results (SEER) database from 2010 to 2016. Prognostic predictors were identified by propensity score matching (PSM) analysis combined with univariate cumulative incidence function (CIF) and multivariate Fine and Gray competitive risk analyses.

Results: Among the women with nonmetastatic TNBC included in the unmatched cohort, a total of 5,375 (18.2%) subjects had prior cancers (P-TNBC) and 24,219 (81.8%) had no prior cancer (NP-TNBC). Patients with P-TNBC tended to have poorer BCSD (Gray's test, =0.0131) and OCSD (Gray's test, =0.0009) in comparison with those with NP-TNBC after PSM. However, the risk of BCSD (=0.291) and OCSD (=0.084) found no difference among P-TNBC patients with one prior cancer and two or more prior cancers after PSM. Additionally, subjects with younger age, advanced T stage, advanced N stage, and advanced differentiation grade tumors were likely to develop BCSD, whereas those with breast-conserving surgery (BCS), radiotherapy, or chemotherapy tended to have a lower incidence of BCSD.

Conclusion: Our study demonstrated that prior cancer was related to the worse BCSD and OCSD rate and could be identified as a reliable survival predictor for patients with nonmetastatic TNBC. This study may provide some reference value for the treatment mode of TNBC patients with prior cancer in the future.
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http://dx.doi.org/10.1155/2021/9988624DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8464407PMC
September 2021

A Novel On-Chip Liquid-Metal-Enabled Microvalve.

Micromachines (Basel) 2021 Aug 30;12(9). Epub 2021 Aug 30.

Liquid Metal and Cryogenic Biomedical Research Center, Technical Institute of Physics and Chemistry, Chinese Academy of Sciences, 29 Zhongguancun East Road, Haidian District, Beijing 100019, China.

A room temperature liquid metal-based microvalve has been proposed in this work. The microvalve has the advantages of easy fabrication, high flexibility, and a low leak rate. By designing a posts array in the channel, the liquid metal can be controlled to form a deformable valve boss and block the flow path. Besides, through adjustment of the pressure applied to the liquid metal, the microvalve can perform reliable switching commands. To eliminate the problem that liquid metal is easily oxidized, which causes the microvalve to have poor repeatability, a method of electrochemical cathodic protection has been proposed, which significantly increases the number of open/close switch cycles up to 145. In addition, this microvalve overcomes the shortcomings of the traditional microvalve that requires an alignment process to assemble all the parts. When the valve is closed, no leak rate is detected at ≤320 mbar, and the leak rate is ≤0.043 μL/min at 330 mbar, which indicates it has good tightness. As an application, we also fabricate a chip that can control bubble flow based on this microvalve. Therefore, this microvalve has great prospects in the field of microfluidics.
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http://dx.doi.org/10.3390/mi12091051DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8467270PMC
August 2021

Mitochondrion-Anchored Photosensitizer with Near Infrared-I Aggregation-Induced Emission for Near Infrared-II Two-Photon Photodynamic Therapy.

Adv Healthc Mater 2021 Sep 26:e2101056. Epub 2021 Sep 26.

National Engineering Research Center for Nanomedicine, College of Life Science and Technology, Huazhong University of Science and Technology, Wuhan, 430074, P. R. China.

Two-photon photodynamic therapy (2P-PDT) that employs photosensitizers (PSs) with 2P absorption is particularly intriguing in cancer treatment, in that 2P excitation enables precise spatial localization and deep tissue penetration. Here, a donor-π-acceptor PS (named TPBPy) with near infrared (NIR) aggregation-induced emission (AIE) is designed and synthesized for imaging-guided 2P-PDT. The maximal photoluminescence (PL) peak of TPBPy is as high as 720 nm when it is encapsulated in liposomes. Upon 2P irradiation by a laser in NIR-II window (λ = 1000 nm), TPBPy exhibits strong NIR-I PL in a multicellular tumor spheroids (MCTSs) model, showing an imaging depth of 210 µm that is significantly higher than upon one-photon irradiation. Moreover, TPBPy localizes specifically on mitochondrion, an important organelle in cell oxidative metabolism and apoptosis. When exposed to the NIR-II irradiation, TPBPy can efficiently generate singlet oxygen ( O ) and trigger cell death. The efficacy of TPBPy-mediated 2P-PDT has also been validated using 4T1 tumor mouse model, the growth of which is significantly suppressed upon NIR-II laser irradiation. TPBPy herein serves as an excellent candidate to suppress deep tumor tissues through NIR-II 2P-PDT, and also renders a new paradigm to construct mitochondrion-anchored AIE luminogens for future cancer theranostic applications.
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http://dx.doi.org/10.1002/adhm.202101056DOI Listing
September 2021

A novel immune subtype classification of ER-positive, PR-negative and HER2-negative breast cancer based on the genomic and transcriptomic landscape.

J Transl Med 2021 Sep 20;19(1):398. Epub 2021 Sep 20.

Department of Breast Surgery, The First Affiliated Hospital of Xi'an Jiaotong University, 277 West Yanta Road, 710061, Xi'an, People's Republic of China.

Background: The diversity and plasticity behind ER+/PR-/HER2- breast cancer have not been widely explored. It is essential to identify heterogeneous microenvironment phenotypes and investigate specific genomic events driving the formation of these phenotypes.

Methods: Based on the immune-related gene expression profiles of 411 ER+/PR-/HER2- breast cancers in the METABRIC cohort, we used consensus clustering to identify heterogeneous immune subtypes and assessed their reproducibility in an independent meta-cohort including 135 patients collected from GEO database. We further analyzed the differences of cellular and molecular characteristics, and potential immune escape mechanism among immune subtypes. In addition, we constructed a transcriptional trajectory to visualize the distribution of individual patient.

Results: Our analysis identified and validated five reproducible immune subtypes with distinct cellular and molecular characteristics, potential immune escape mechanisms, genomic drivers, as well as clinical outcomes. An immune-cold subtype, with the least amount of lymphocyte infiltration, had a poorer prognosis. By contrast, an immune-hot subtype, which demonstrated the highest infiltration of CD8+ T cells, DCs and NK cells, and elevated IFN-γ response, had a comparatively favorable prognosis. Other subtypes showed more diverse gene expression and immune infiltration patterns with distinct clinical outcomes. Finally, our analysis revealed a complex immune landscape consisting of both discrete cluster and continuous spectrum.

Conclusion: Overall, this study revealed five heterogeneous immune subtypes among ER+/PR-/HER2- breast cancer, also provided important implications for clinical translations.
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http://dx.doi.org/10.1186/s12967-021-03076-xDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8454077PMC
September 2021

Microfluidic single-cell transcriptomics: moving towards multimodal and spatiotemporal omics.

Lab Chip 2021 10 12;21(20):3829-3849. Epub 2021 Oct 12.

The MOE Key Laboratory of Spectrochemical Analysis & Instrumentation, The Key Laboratory of Chemical Biology of Fujian Province, State Key Laboratory of Physical Chemistry of Solid Surfaces, Department of Chemical Biology, College of Chemistry and Chemical Engineering, Xiamen University, Xiamen 361005, China.

Cells are the basic units of life with vast heterogeneity. Single-cell transcriptomics unveils cell-to-cell gene expression variabilities, discovers novel cell types, and uncovers the critical roles of cellular heterogeneity in biological processes. The recent advances in microfluidic technologies have greatly accelerated the development of single-cell transcriptomics with regard to throughput, sensitivity, cost, and automation. In this article, we review state-of-the-art microfluidic single-cell transcriptomics, with a focus on the methodologies. We first summarize six typical microfluidic platforms for isolation and transcriptomic analysis of single cells. Then the on-going trend of microfluidic transcriptomics towards multimodal omics, which integrates transcriptomics with other omics to provide more comprehensive pictures of gene expression networks, is discussed. We also highlight single-cell spatial transcriptomics and single-cell temporal transcriptomics that provide unprecedented spatiotemporal resolution to reveal transcriptomic dynamics in space and time, respectively. The emerging applications of microfluidic single-cell transcriptomics are also discussed. Finally, we discuss the current challenges to be tackled and provide perspectives on the future development of microfluidic single-cell transcriptomics.
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http://dx.doi.org/10.1039/d1lc00607jDOI Listing
October 2021

Color-Tunable Alternating-Current Quantum Dot Light-Emitting Devices.

ACS Appl Mater Interfaces 2021 Sep 14;13(38):45815-45821. Epub 2021 Sep 14.

Key Lab of Physics and Technology for Advanced Batteries (Ministry of Education), College of Physics, Jilin University, Changchun 130012, China.

To date, it remains a central challenge to achieve electroluminescence in both positive and negative half cycles of alternating-current (AC) voltage for a light-emitting device. Herein, we successfully demonstrated a novel structure to construct a real AC quantum dot light-emitting device (QLED) with two charge generation layers (CGLs) consisting of the poly(3,4-ethylenedioxythiophene):poly(styrene sulfonate)/ZnO nanoparticle bilayer structure. Besides the conventional driving way with power input from a pair of opposite electrodes, this AC QLED can also work in the manner of in-planar-electrode driving mode, achieving simultaneous electroluminescence of each pixel. By employing a bilayer emissive layer composed of red and green quantum dots, the emission color of the AC QLED can be tuned by both the polarity and amplitude of the driving voltage. Leveraging the excellent electron injection and negligible voltage consumption from the CGLs, this QLED can be turned on at a record low voltage of 5.6 V. We believe that this AC QLED can provide a platform for the realization of simple and smart plug-and-play QLED-based display and lighting systems.
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http://dx.doi.org/10.1021/acsami.1c11529DOI Listing
September 2021

Optimization of 4,6-Disubstituted Pyrido[3,2-]pyrimidines as Dual MNK/PIM Inhibitors to Inhibit Leukemia Cell Growth.

J Med Chem 2021 Sep 13;64(18):13719-13735. Epub 2021 Sep 13.

Key Laboratory of Structure-Based Drugs Design & Discovery of Ministry of Education, Shenyang Pharmaceutical University, Shenyang 110016, China.

Mitogen-activated protein kinase-interacting kinases (MNKs) and provirus integration in maloney murine leukemia virus kinases (PIMs) are downstream enzymes of cell proliferation signaling pathways associated with the resistance of tyrosine kinase inhibitors. MNKs and PIMs have complementary effects to regulate cap-dependent translation of oncoproteins. Dual inhibitors of MNKs and PIMs have not been developed. We developed a novel 4,6-disubstituted pyrido[3,2-]pyrimidine compound with selective inhibition of MNKs and PIMs. The IC's of to inhibit MNK1 and MNK2 are 1 and 7 nM and those to inhibit PIM1, PIM2, and PIM3 are 43, 232, and 774 nM, respectively. inhibits the growth of myeloid leukemia K562 and MOLM-13 cells with GI's of 2.1 and 1.2 μM, respectively. decreases the levels of -eIF4E and -4EBP1, the downstream products of MNKs and PIMs, as well as cap-dependent proteins c-myc, cyclin D1, and Mcl-1. inhibits the growth of MOLM-13 cell xenografts without causing evident toxicity. represents an innovative dual MNK/PIM inhibitor with a good pharmacokinetic profile.
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http://dx.doi.org/10.1021/acs.jmedchem.1c01084DOI Listing
September 2021

Distinctive Gut Microbiota in Patients with Overweight and Obesity with Dyslipidemia and its Responses to Long-term Orlistat and Ezetimibe Intervention: A Randomized Controlled Open-label Trial.

Front Pharmacol 2021 26;12:732541. Epub 2021 Aug 26.

Department of Endocrinology, West China Hospital of Sichuan University, Chengdu, China.

This study investigated the gut microbiota and short chain fatty acids (SCFAs) characteristics of subjects with obesity from Xinjiang in northwestern China, a region with a multiethnic culture and characteristic lifestyle, and to explore the potential microbes that respond to a 12-wk medication of orlistat and ezetimibe with a randomized controlled open-label trial manner. The gut microbiota profile of patients with overweight and obesity with dyslipidemia in Xinjiang was distinctive and characterized by enrichment of and the reduction of the diversity and the depletion of , , , and . -type, -type, and -type were the gut microbial patterns of the Xinjiang population. However, the fecal SCFAs levels and enterotypes were similar between healthy individuals and patients. These results indicated that the contribution of the gut microbiota to obesity was highly dependent on geography and dietary habits. Waist circumference, total triglyceride (TG), and fasting blood glucose (FBG) were significantly decreased after orlistat therapy, whereas TG, total cholesterol (TC), and low density lipoprotein cholesterol (LDL-C) were significantly decreased by ezetimibe. Overall, the gut microbiota and their SCFAs metabolites were relatively stable after treatment with the two drugs, with alteration of some low-abundant bacteria, i.e., significantly increased and decreased after orlistat, and increased and after ezetimibe therapy. These results indicated that intestinal malabsorption of dietary fat and cholesterol caused by orlistat and ezetimibe had a limited effect on the overall gut microbial community and their metabolites. Nevertheless, significant correlations between several core microbes that responded to the medications and biochemical data were found; in particular, and were positively correlated with FBG after orlistat intervention, while and were negatively correlated with TC and LDL-C after ezetimibe intervention, thus indicating their roles in improving glucolipid metabolism in obesity by acting as potential microbial targets.
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http://dx.doi.org/10.3389/fphar.2021.732541DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8426637PMC
August 2021

Design, synthesis and biological evaluation of novel hybrids targeting mTOR and HDACs for potential treatment of hepatocellular carcinoma.

Eur J Med Chem 2021 Sep 3;225:113824. Epub 2021 Sep 3.

Guangdong Provincial Key Laboratory of Fermentation and Enzyme Engineering, Joint International Research Laboratory of Synthetic Biology and Medicine, Guangdong Provincial Engineering and Technology Research Center of Biopharmaceuticals, School of Biology and Biological Engineering, South China University of Technology, Guangzhou, 510006, China. Electronic address:

Hepatocellular carcinoma (HCC) is a major contributor to global cancer incidence and mortality. Many pathways are involved in the development of HCC and various proteins including mTOR and HDACs have been identified as potential drug targets for HCC treatment. In the present study, two series of novel hybrid molecules targeting mTOR and HDACs were designed and synthesized based on parent inhibitors (MLN0128 and PP121 for mTOR, SAHA for HDACs) by using a fusion-type molecular hybridization strategy. In vitro antiproliferative assays demonstrated that these novel hybrids with suitable linker lengths exhibited broad cytotoxicity against various cancer cell lines, with significant activity against HepG2 cells. Notably, DI06, an MLN0128-based hybrid, exhibited antiproliferative activity against HepG2 cells with an IC value of 1.61 μM, which was comparable to those of both parent drugs (MLN0128, IC = 2.13 μM and SAHA, IC = 2.26 μM). In vitro enzyme inhibition assays indicated that DI06, DI07 and DI17 (PP121-based hybrid) exhibited nanomolar inhibitory activity against mTOR kinase and HDACs (e.g., HDAC1, HDAC2, HDAC3, HADC6 and HADC8). Cellular studies and western blot analyses uncovered that in HepG2 cells, DI06 and DI17 induced cell apoptosis by targeting mTOR and HDACs, blocked the cell cycle at the G0/G1 phase and suppressed cell migration. The potential binding modes of the hybrids (DI06 and DI17) with mTOR and HDACs were investigated by molecular docking. DI06 displayed better stability in rat liver microsomes than DI07 and DI17. Collectively, DI06 as a novel mTOR and HDACs inhibitor presented here warrants further investigation as a potential treatment of HCC.
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http://dx.doi.org/10.1016/j.ejmech.2021.113824DOI Listing
September 2021

Correction: Efficacy of omega-3 PUFAs in depression: A meta-analysis.

Transl Psychiatry 2021 Sep 7;11(1):465. Epub 2021 Sep 7.

Mood Disorders Psychopharmacology Unit, University Health Network; Department of Psychiatry, University of Toronto; Institute of Medical Science, University of Toronto; Department of Pharmacology, University of Toronto, Toronto, Ontario, Canada.

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http://dx.doi.org/10.1038/s41398-021-01582-6DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8423804PMC
September 2021

A Chinese pedigree with glucocorticoid remediable aldosteronism.

Hypertens Res 2021 Aug 30. Epub 2021 Aug 30.

Department of Hypertension, Fuwai Hospital, State Key Laboratory of Cardiovascular Disease, National Center for Cardiovascular Diseases, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, China.

Glucocorticoid-remediable aldosteronism (GRA) is an autosomal-dominant inherited aldosteronism that is often accompanied by early-onset hypertension. GRA is caused by the unequal crossover of the 11β-hydroxylase (CYP11B1) and aldosterone synthase (CYP11B2) genes. As a result of chimeric gene duplication, aldosterone is ectopically synthesized in the adrenal zona fasciculata under the control of adrenocorticotropic hormone (ACTH). Here, we describe a Chinese pedigree with three affected subjects. Both the uncle and nephew were hospitalized in our hospital due to early-onset hypertension (onset <20 years old) and were diagnosed with primary aldosteronism (PA). Their laboratory test results revealed hyperaldosteronism, hyporeninemia, a high plasma aldosterone to renin (ARR) ratio, and normal serum potassium (K). Captopril failed to suppress aldosterone secretion. This family had a strong paternal history of hypertension. Thirteen members underwent gene testing, and three of them were found to be GRA positive. Through long-extension PCR (XL-PCR) and direct sequencing, we identified the CYP11B1/CYP11B2 chimeric gene, and with unequal crossover breakpoints located between intron 2 of CYP11B1 and exon 3 of CYP11B2 in the three patients. Low-dose dexamethasone was effective. This is the first family report of GRA in northern China. Moreover, a case of GRA combined with a CACNA1H gene mutation is reported for the first time. We found that dihydropyridine calcium channel blockers (CCBs) combined with aldosterone receptor antagonists exerted good therapeutic effects in controlling blood pressure in GRA patients for whom glucocorticoid therapy was not an option.
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http://dx.doi.org/10.1038/s41440-021-00685-3DOI Listing
August 2021

Junctional complexes in epithelial cells: sentinels for extracellular insults and intracellular homeostasis.

FEBS J 2021 Aug 28. Epub 2021 Aug 28.

Jiangsu Key Laboratory of Infection and Immunity, Institutes of Biology and Medical Sciences, Soochow University, China.

The cell-cell and cell-ECM junctions within the epithelial tissues are crucial anchoring structures that provide architectural stability, mechanical resistance, and permeability control. Their indispensable role as signaling hubs orchestrating cell shape-related changes such as proliferation, differentiation, migration, and apoptosis has also been well recognized. However, growing amount of evidence now suggests that the multitasking nature of epithelial junctions extends well beyond anchorage-dependent or cell shape change-related biological processes. In this review, we discuss the emerging roles of junctional complexes in regulating innate immune defense, stress resistance, and intracellular proteostasis of the epithelial cells, with emphasis on the upstream regulation of epithelial junctions on various aspects of the epithelial barrier.
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http://dx.doi.org/10.1111/febs.16174DOI Listing
August 2021

Herb Sanqi-Derived Compound K Alleviates Oxidative Stress in Cultured Human Melanocytes and Improves Oxidative-Stress-Related Leukoderma in Guinea Pigs.

Cells 2021 Aug 11;10(8). Epub 2021 Aug 11.

Department of Pigmentation Research and Therapeutics, Graduate School of Medicine, Osaka City University, Osaka 5450051, Japan.

Sanqi, a traditional Chinese herb, is widely used for cardiovascular diseases, and its neuroprotective effects against oxidative stress were recently discovered. The purpose of this study was to investigate whether Sanqi-derived compound K (Sanqi-CK), an active metabolite of Sanqi, could protect melanocytes from oxidative stress. Cultured human primary skin epidermal melanocytes (HEMn-MPs) were treated with hydrogen peroxide (HO) in the presence or absence of Sanqi-CK. Sanqi-CK exhibited protective effects against HO-induced cell death by reducing oxidative stress. In addition, treatment with Sanqi-CK reversed the decreased glutathione reductase activity and decreased ratio of reduced glutathione (GSH)/oxidized glutathione (GSSG) seen in HO-treated melanocytes. Furthermore, topical application of Sanqi-CK alleviated leukoderma in guinea pigs, a disorder characterized by melanocyte cell death resulting from rhododendrol-induced oxidative stress. Taken together, these data suggest that Sanqi-CK protects melanocytes against oxidative stress, and its protective effects are associated with modulating the redox balance between GSH and GSSG and activating glutathione reductase. Thus, Sanqi-CK may be a good candidate for preventing melanocyte loss in oxidative-stress-associated pigmentary disorders.
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http://dx.doi.org/10.3390/cells10082057DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8393903PMC
August 2021

Arabidopsis SIGMA FACTOR BINDING PROTEINs function antagonistically to WRKY75 in abscisic acid-mediated leaf senescence and seed germination.

J Exp Bot 2021 Aug 26. Epub 2021 Aug 26.

CAS Key Laboratory of Tropical Plant Resources and Sustainable Use, Xishuangbanna Tropical Botanical Garden, Chinese Academy of Sciences, Mengla, Yunnan 666303, China.

The plant specific VQ gene family participates in diverse physiological processes but little information is available on their role in leaf senescence. Here, we show that the VQ motif-containing proteins, Arabidopsis SIGMA FACTOR BINDING PROTEIN1 (SIB1) and SIB2 are negative regulators of ABA-mediated leaf senescence. Loss of SIB1 and SIB2 functions resulted in increased sensitivity in ABA-induced leaf senescence. In contrast, overexpression of SIB1 significantly delayed this process. Moreover, biochemical studies revealed that SIBs interact with the WRKY75 transcription factor. Loss of WRKY75 functions decreased sensitivity to ABA-induced leaf senescence, while overexpression of WRKY75 significantly accelerated this process. Chromatin immunoprecipitation assays revealed that WRKY75 directly binds to the promoters of GOLDEN 2-LIKE1 (GLK1) and GLK2, repressing expression. SIBs repress the transcriptional functions of WRKY75 and negatively regulate ABA-induced leaf senescence in a WRKY75-dependent manner. In contrast, WRKY75 positively regulates ABA-mediated leaf senescence in a GLK-dependent manner. In addition, SIBs function antagonistically to WRKY75 in ABA-mediated seed germination. These results demonstrate that SIBs can form a complex with WRKY75 to antagonistically regulate ABA-mediated leaf senescence and seed germination.
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http://dx.doi.org/10.1093/jxb/erab391DOI Listing
August 2021

Mid-aortic syndrome is associated with increased left ventricular mass index in Takayasu arteritis.

Ann Transl Med 2021 Jul;9(14):1124

Department of Cardiology, Fuwai Hospital, National Center for Cardiovascular Disease, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, China.

Background: Mid-aortic syndrome (MAS) may induce changes in cardiac structure among patients with Takayasu arteritis (TA).

Methods: Consecutive adult patients with TA (January 1, 2011 to January 1, 2018) were enrolled and their data was retrospectively analyzed.

Results: Patients were divided into MAS group (100/457 patients, 21.8%) and non-MAS group (357, 78.1%). The left ventricular mass index (LVMI) was higher in the MAS group than the non-MAS (113.78±26.82 versus 100.74±23.66 g/m, respectively; P<0.001). The MAS group showed higher prevalence than the non-MAS group of mild-to-severe mitral regurgitation (9.0% and 3.9%, respectively; P=0.040) and aortic regurgitation (26% and 14.8%, respectively; P=0.003). No difference was found in the rates of heart failure (27.0% and 19.9% for MAS and non-MAS, respectively; P=0.126). The MAS group also showed lower estimated glomerular filtration rates than the non-MAS group (89.93±18.89 versus 96.16±21.60 mL/min/1.73 m, respectively; P=0.009) and higher prevalence of renal artery stenosis (57% versus 43.7%; P=0.018). MAS was independently related to greater LVMI in both unadjusted model [β=12.60; 95% confidence interval (CI): 7.09-18.11; P<0.001] and the model adjusted for multiple indices (β=9.91; 95% CI: 4.57-15.25; P<0.001) in multivariate linear analysis. The LVMI significantly decreased from 111.49±25.65 to 100.36±22.91 g/m (P<0.001) among 55 patients who underwent successful revascularization treatment for MAS, while no significant difference (P=0.635) was observed among patients treated with medicine alone.

Conclusions: TA-induced MAS is a potential independent risk factor for increased LVMI, and revascularization therapy for MAS is effective in reversing structural changes in the heart.
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http://dx.doi.org/10.21037/atm-20-7508DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8350696PMC
July 2021

Efficacy evaluation and mechanical study of short- and long-term antithrombotic therapy for Kawasaki disease.

Transl Pediatr 2021 Jul;10(7):1883-1889

Department of Cardiology, Hebei Children's Hospital, Shijiazhuang, China.

Background: Antithrombotic therapy was commonly applied in treating Kawasaki disease (KD) children; however, the effects and mechanisms of different plans were not fully elucidated. In this study, we aimed to evaluate different antithrombotic drugs.

Methods: Eighty-two children diagnosed with KD in Hebei Children's Hospital from January 2017 to January 2020 were recruited. For cohort study, KD children were divided into a series of groups according to whether they were complicated with coronary artery lesions (CAL), drug therapy plan, and the presence of liver damage. The thromboelastogram (TEG) indexes [clotting time (R), clot formation time (K), clot formation angle (α), maximum amplitude of the clot (MA), arachidonic acid (AA), and adenosine diphosphate (ADP)] were employed to evaluate the relationship between disease state and drug treatment efficacy. Meanwhile, children were divided into different therapy groups according to their degree of CAL, the treatment efficiency was evaluated by TEG indexes, and the bleeding ratio was recorded. In addition, the warfarin metabolic gene was detected to explain the changes of coagulation parameters in children treated with warfarin.

Results: The R value and coagulation index (CoI) were significantly lower (P<0.05) and MA value was significantly higher (P<0.05) in CAL group than those in non-coronary artery lesion (NCAL) group. There were significant individual differences in platelet inhibition between aspirin and dipyridamole groups. The AA% in aspirin group was 64% [95% confidence interval (CI): 49% to 74.3%] and the ADP% was 28.5% (95% CI: 26.2% to 37.2%) in dipyridamole group, and were significantly between groups. Warfarin and aspirin had a synergistic effect in anticoagulation. Warfarin metabolic gene detection was shown to be helpful for adjusting warfarin treatment dose, and shortening the initial attainment time of standard international normalized ratio (INR) index. The R value was significantly higher (P<0.05) in the liver injury group than that in the control group.

Conclusions: KD Children complicated with CAL presented a hypercoagulable state, and the CAL was predictable with the R value. Warfarin metabolic gene detection and TEG can effectively evaluate and guide short- and long-term antithrombotic therapy in KD children.
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http://dx.doi.org/10.21037/tp-21-299DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8349964PMC
July 2021

Patterns and drivers of global gross nitrogen mineralization in soils.

Glob Chang Biol 2021 Nov 29;27(22):5950-5962. Epub 2021 Aug 29.

Department of Renewable Resources, University of Alberta, Edmonton, Canada.

Soil gross nitrogen (N) mineralization (GNM), a key microbial process in the global N cycle, is mainly controlled by climate and soil properties. This study provides for the first time a comprehensive analysis of the role of soil physicochemical properties and climate and their interactions with soil microbial biomass (MB) in controlling GNM globally. Through a meta-analysis of 970 observations from 337 published papers from various ecosystems, we found that GNM was positively correlated with MB, total carbon, total N and precipitation, and negatively correlated with bulk density (BD) and soil pH. Our multivariate analysis and structural equation modeling revealed that GNM is driven by MB and dominantly influenced by BD and precipitation. The higher total N accelerates GNM via increasing MB. The decrease in BD stimulates GNM via increasing total N and MB, whereas higher precipitation stimulates GNM via increasing total N. Moreover, the GNM varies with ecosystem type, being greater in forests and grasslands with high total carbon and MB contents and low BD and pH compared to croplands. The highest GNM was observed in tropical wet soils that receive high precipitation, which increases the supply of soil substrate (total N) to microbes. Our findings suggest that anthropogenic activities that affect soil microbial population size, BD, soil substrate availability, or soil pH may interact with changes in precipitation regime and land use to influence GNM, which may ultimately affect ecosystem productivity and N loss to the environment.
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http://dx.doi.org/10.1111/gcb.15851DOI Listing
November 2021

Mitigation of greenhouse gas emissions from a red acidic soil by using magnesium-modified wheat straw biochar.

Environ Res 2021 Aug 11;203:111879. Epub 2021 Aug 11.

Department of Soil Science and Agricultural Chemistry, Engineering Polytechnic School, Campus Univ. s/n, University of Santiago de Compostela, 27002, Lugo, Spain.

To mitigate greenhouse gas (GHG) emissions, different strategies have been proposed, including application of dolomite, crop straw and biochar, thus contributing to cope with the increasing global warming affecting the planet. In the current study, pristine wheat straw biochar (WBC) and magnesium (MgCl.6HO) modified wheat straw biochar (MWBC) were used. Treatments included control (CK), two WBC dosages (1% and 2.5%), and two MWBC doses (1% and 2.5%). After 90 days of incubation, WBC and MWBC improved the soil physiochemical properties, being more pronounced with increasing rates of biochar. MWBC significantly decreased microbial biomass carbon (MBC), while microbial biomass nitrogen (MBN) increased when both biochar materials (WBC and MWBC) were applied at low rate. Compared to control soil, Urease and Alkaline phosphatase activities increased with the increasing rate of WBC and MWBC. The activities of dehydrogenase and β-glucosidase decreased with the WBC and MWBC application, compared to CK. The fluxes of all the three GHGs evaluated (CO, CH and NO) decreased with time for both biochar amendments, while cumulative emission of CO increased by 58% and 45% for WBC, and by 54% and 41% for MWBC, as compared to CK. The NO cumulative emissions decreased by 18 and 34% for WBC, and by 25 and 41% for MWBC, compared to CK, whereas cumulative methane emission showed non-significant differences among all treatments. These findings indicate that Mg-modified wheat straw biochar would be an appropriate management strategy aiding to reduce GHG emissions and improving the physiochemical properties of affected soils, and specifically of the red dry land soil investigated in the current work.
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http://dx.doi.org/10.1016/j.envres.2021.111879DOI Listing
August 2021

Predicting Rate Constants of Hydroxyl Radical Reactions with Alkanes Using Machine Learning.

J Chem Inf Model 2021 09 11;61(9):4259-4265. Epub 2021 Aug 11.

Key Laboratory of Magnetic Resonance in Biological Systems, State Key Laboratory of Magnetic Resonance and Atomic and Molecular Physics, National Center for Magnetic Resonance in Wuhan, Wuhan Institute of Physics and Mathematics, Innovation Academy for Precision Measurement Science and Technology, Chinese Academy of Sciences, Wuhan 430071, China.

The hydrogen abstraction reactions of the hydroxyl radical with alkanes play an important role in combustion chemistry and atmospheric chemistry. However, site-specific reaction constants are difficult to obtain experimentally and theoretically. Recently, machine learning has proved its ability to predict chemical properties. In this work, a machine learning approach is developed to predict the temperature-dependent site-specific rate constants of the title reactions. Multilayered neural network (NN) models are developed by training the site-specific rate constants of 11 reactions, and several schemes are designed to improve the prediction accuracy. The results show that the proposed NN models are robust in predicting the site-specific and overall rate constants.
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http://dx.doi.org/10.1021/acs.jcim.1c00809DOI Listing
September 2021

Bowel ultrasound enhances predictive value based on clinical indicators: a scoring system for moderate-to-severe endoscopic activities in patients with ulcerative colitis.

Therap Adv Gastroenterol 2021 13;14:17562848211030050. Epub 2021 Jul 13.

Department of Gastroenterology, Peking Union Medical College Hospital, No.1 Shuaifuyuan, Wangfujing Street, Dongcheng District, Beijing, 100730, China.

Background And Aim: The aim was to assess non-invasive factors among clinical features, laboratory, and bowel ultrasound (BUS) characteristics and to develop a scoring system to predict endoscopic activities for ulcerative colitis (UC) patients.

Methods: We performed a retrospective study collecting UC patients between January 2015 to September 2020. Logistic regression was performed to predict moderate-to-severe endoscopic activities, defined as endoscopic Mayo score ⩾2. Model performance was described with discrimination and calibration ability and validated by internal and external methods.

Results: A total of 103 and 29 patients were enrolled in the modeling and validation groups, respectively. Stool frequency ⩾5 times/day, hematochezia, erythrocyte sedimentation rate (ESR), and colonic wall flow in BUS were included into two predictive models for endoscopic activities, both with good discrimination ability [Area under curve (AUC) 0.879 and 0.882,   0.001] and a sensitivity of 76.7% and specificity of 92.3%, which showed an adequate calibration ability by using the Hosmer-Lemeshow test ( = 0.14 and 0.07). The external validation displayed consistent results with the above mentioned. Nomograms were also established for these models.

Conclusion: We developed predictive models for endoscopic disease activities by using noninvasive factors based on stool frequency, hematochezia, ESR, and colonic wall blood flow in BUS. These models performed well in the internal and external validation.
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http://dx.doi.org/10.1177/17562848211030050DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8283041PMC
July 2021
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