Publications by authors named "Huimin Sun"

131 Publications

Bioinspired surface-enhanced Raman scattering substrate with intrinsic Raman signal for the interactive SERS detection of pesticides residues.

Spectrochim Acta A Mol Biomol Spectrosc 2021 Dec 25;270:120800. Epub 2021 Dec 25.

Department of Microelectronic Science and Engineering, School of Physical Science and Technology, Ningbo University, Ningbo, Zhejiang 315211, PR. China. Electronic address:

Although biomimetic surface enhanced Raman scattering (SERS) substrate which makes use of naturally existing raw materials have been fully utilized, it remains challenging to achieve credible quantitative detection. Herein, nanoimprint technology was exploited to engineer internal standard (IS) enabled quantitative flexible biomimetic SERS substrates, in which polydimethylsiloxane (PDMS) with intrinsic Raman signal was utilized as a tool to reversely duplicate surface structures from different agriculture products and then deposited with Ag nanoparticles. The resultant four kinds of biomimetic SERS substrates with different surface geometries all permit highly sensitive assay with enhancement factors (EFs) of about 10 in both drop-dry and in situ SERS detection modes. Moreover, the quantitative degree in the SERS detection was effectively corrected based on the IS strategy. Finally, an ingenious interactive in situ SERS detection was conducted. Interestingly, the maximum recovery rate was achieved when the template food was used as target surface compared with other foods, indicating the significance of manufacturing the highly conformed SERS-active structure from the surface to be tested. The proposed quantitative biomimetic SERS substrate is expected to be widely used in the field of biochemical supervision.
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http://dx.doi.org/10.1016/j.saa.2021.120800DOI Listing
December 2021

RING E3 ubiquitin ligase TaSADR1 negatively regulates drought resistance in transgenic Arabidopsis.

Plant Physiol Biochem 2022 Jan 8;170:255-265. Epub 2021 Dec 8.

College of Agronomy, Northwest A&F University, Yangling, Shaanxi, 712100, People's Republic of China. Electronic address:

Drought stress is an important factor that affects crop yields and quality. E3 ubiquitin ligase has crucial roles in the responses to abiotic stresses. However, few studies have investigated the role of E3 ubiquitin ligase during drought stress in wheat. In this study, we cloned and identified the orthologous gene of Oryza sativa Salt-, ABA- and Drought-Induced RING Finger Protein 1 (OsSADR1) in wheat (Triticum aestivum L.) called TaSADR1. TaSADR1 encodes a protein containing 486 amino acids with a C3HC4 type RING finger conserved domain at the N-terminal. We confirmed that TaSADR1 has an E3 ubiquitin ligase activity and it is located in the nucleus. High expression of TaSADR1 was induced by treatment with PEG6000 and abscisic acid (ABA). TaSADR1-overexpressing transgenic Arabidopsis plants exhibited decreased drought tolerance. Under drought stress, compared with the wild-type (WT) lines, TaSADR1-overexpressing transgenic Arabidopsis lines had lower proline and chlorophyll contents, and antioxidant enzyme activities (superoxide dismutase, peroxidase, and catalase), whereas the water loss rate, malondialdehyde content, and relative electrolyte leakage were higher. In addition, the overexpressing transgenic Arabidopsis lines were more sensitive to mannitol and ABA treatment at seed germination and during seedling growth. The expression levels of genes related to stress were downregulated under drought conditions in the transgenic plants. Our results demonstrate that TaSADR1 may negatively regulate drought stress responses by regulating the expression of stress-related genes.
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http://dx.doi.org/10.1016/j.plaphy.2021.12.004DOI Listing
January 2022

Removal of inhibitory furan aldehydes in lignocellulosic hydrolysates via chitosan-chitin nanofiber hybrid hydrogel beads.

Bioresour Technol 2021 Dec 12;346:126563. Epub 2021 Dec 12.

Jiangsu Co-Innovation Center of Efficient Processing and Utilization of Forest Resources, College of Chemical Engineering, Nanjing Forestry University, Nanjing 210037, People's Republic of China.

To obtain fermentable sugars from lignocellulose, various inhibitors, especially furan aldehydes, are usually generated during the pretreatment process. These inhibitors are harmful to subsequent microbial growth and fermentation. In this study, a novel detoxification strategy was proposed to remove 5-hydroxymethylfurfural (HMF) and furfural while retaining glucose and xylose using self-prepared chitosan-chitin nanofiber hybrid hydrogel beads (C-CNBs). After C-CNBs treatment, the removal rates of HMF and furfural from sugarcane bagasse hydrolysates reached 63.1% and 68.4%, while the loss rates of glucose and xylose were only 6.3% and 8.2%, respectively. Two typical industrial strains grew well in monosaccharide-rich detoxified hydrolysates, with a specific growth rate at least 4.1 times that of undetoxified hydrolysates. Furthermore, adsorption mechanism analysis revealed that the Schiff base reaction and mesopore filling were involved in furan aldehyde adsorption. In total, C-CNBs provide an efficient and practical approach for the removal of furan aldehydes from lignocellulosic hydrolysates.
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http://dx.doi.org/10.1016/j.biortech.2021.126563DOI Listing
December 2021

Effects of physicochemical factors on the transport of aged polystyrene nanoparticles in saturated porous media.

Chemosphere 2022 Feb 9;289:133239. Epub 2021 Dec 9.

Agro-Environmental Protection Institute, Agro-Environmental Protection Institute, Ministry of Agriculture and Rural Affairs of the People's Republic of China Tianjin, 300191, China.

Plastic debris, particularly nanoplastics, have attracted substantial attention as an emerging pollutant of global concern. The aging process caused by UV could dramatically alter the physicochemical properties of polystyrene plastics and affect their fate and transport in the subsurface environment. We researched the effects of diverse factors, including flow rate, ionic strength (IS), and cation valence on the transport of aged polystyrene nanoparticles (PSNPs) in saturated porous media and found that their mobility was greatly enhanced by the aging process at all other experimental conditions except coexistence with Al. Interestingly, we found that the aged PSNPs were polymerized due to the coexistence with Al, which led the aged PSNPs to exhibit weaker mobility than the pristine. Zeta potential and particle size measurements, FTIR spectroscopy, and XPS were employed to characterize the PSNPs, and the results suggest that UV radiation provides O-containing functional groups for the PSNPs. The experimental results correspond well with the ADR model and the values of Smax and k closely reflect the deposition of the PSNPs in sand columns. Moreover, the Derjaguin-Landau-Verwey-Overbeek (DLVO) theoretical calculation accurately reflects the interaction of the pristine and aged PSNPs and quartz sand. Overall, due to the processes that PSNPs possibly undergo in the environment, their mobility may fluctuate dramatically. These findings help remedy knowledge deficiency regarding nanoplastic mobility being affected by aging processes, further underscore the critical influence of the aging process on the potential risks and environmental fates of nanoplastics.
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http://dx.doi.org/10.1016/j.chemosphere.2021.133239DOI Listing
February 2022

Efficient removal of Cd (II) from aqueous solution by chitosan modified kiwi branch biochar.

Chemosphere 2022 Feb 9;289:133251. Epub 2021 Dec 9.

College of Natural Resources and Environment, Northwest A & F University, Yangling, Shaanxi, 712100, China; Key Laboratory of Plant Nutrition and the Agri-environment in Northwest China, Ministry of Agriculture and Rural Affairs of the People's Republic of China, Yangling, 712100, China. Electronic address:

Production of cost-efficient composite materials from low-cost modified biochar for the removal of Cd (II) from wastewater is much needed to meet the growing needs of industrial wastewater treatments. A novel chitosan-modified kiwi branch biochar (CHKB) was fabricated as low-cost modified biochar for the removal of Cd (II) from aqueous solution. Batch adsorption and characterization experiments indicated that the modification of kiwi biochar (KB) by chitosan remarkably improved its adsorption performance. The results revealed that the adsorption isotherms can be best described by a Langmuir model and that a pseudo-second-order model fits the Cd (II) adsorption kinetics well, which indicates that it is a monolayer process controlled by chemisorption. CHKB exhibited a Langmuir maximum adsorption capacity of Cd (II) (126.58 mg g), whereas that of KB was only 4.26 mg g. The adsorption ability of CHKB was improved by increasing the surface area and an abundance of surface functional groups (-OH, -NH, CO, etc.). The cation exchange, electrostatic interaction, surface complexation, and precipitation were the main mechanisms in the sorption of Cd (II) on CHKB. Excellent adsorption performance, low cost, and environmental-friendliness made CHKB a fantastic adsorbent for the removal of Cd (II) in wastewater.
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http://dx.doi.org/10.1016/j.chemosphere.2021.133251DOI Listing
February 2022

Nighttime sleep duration, restlessness and risk of multimorbidity - A longitudinal study among middle-aged and older adults in China.

Arch Gerontol Geriatr 2021 Nov 15;99:104580. Epub 2021 Nov 15.

Department of Epidemiology, School of Public Health, Sun Yat-sen University, Guangzhou, China. Electronic address:

Purpose: To assess the associations of nighttime sleep duration and restlessness with the risk of multimorbidity in Chinese middle-aged and older adults.

Methods: We used the 2011 and 2015 surveys of the China Health and Retirement Longitudinal Study (CHARLS). Sleep duration was grouped into ≤ 5, (5-6], (6-8], (8-9], and > 9 h/night. Restlessness days in the past week were categorized into < 1, 1-2, 3-4, and 5-7 days/week. Multimorbidity was defined as the co-existence of two or more of 14 chronic conditions (hypertension, dyslipidemia, diabetes mellitus, cancer, chronic lung disease, liver disease, heart problems, stroke, kidney disease, digestive disease, psychiatric problems, memory-related disease, arthritis, and asthma). Log-binomial regression models were used to estimate the associations.

Results: A total of 6,037 participants free of multimorbidity at baseline were included. During four-years of follow-up, 2,203 (36.5%) participants developed multimorbidity. Compared to participants who slept 6-8 h/night, those with short sleep duration ≤ 5 h/night and 5-6 h/night were associated with 33.3% (95% CI: 14.8%-54.7%) and 24.2% (95% CI: 5.9%-45.6%) increased risk of multimorbidity, respectively. Long sleep duration was not significantly associated with incident multimorbidity. Compared to those who rarely or never had a restless sleep in the past week, participants with 5-7 days of restless sleep had increased risk of multimorbidity (RR: 1.750, 95% CI: 1.476-2.076). Similar findings were confirmed in subgroups by age, gender, and baseline chronic condition status.

Conclusions: Short nighttime sleep duration and restlessness were associated with increased risk of multimorbidity in China.
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http://dx.doi.org/10.1016/j.archger.2021.104580DOI Listing
November 2021

Effect of nZVI/biochar nanocomposites on Cd transport in clay mineral-coated quartz sand: Facilitation and rerelease.

Ecotoxicol Environ Saf 2021 Nov 11;228:112971. Epub 2021 Nov 11.

College of Natural Resources and Environment, Northwest A&F University, Yangling, Shaanxi 712100, PR China; Key Laboratory of Plant Nutrition and the Agri-environment in Northwest China, Ministry of Agriculture and Rural Affairs, Yangling, Shaanxi 712100, PR China. Electronic address:

The development and application of nano-biochar synthesized by ball milling technology is still challenging in the field of environmental remediation because of its higher activity with pollutants. The purpose of this study was to investigate the transport behavior of two kinds of biochar nanoparticles (nanobiochar (NBC) and nZVI-modified nanobiochar (nZVI-NBC)) and Cd in clay mineral (kaolinite, illite, and montmorillonite)-coated quartz sand columns. The interaction between biochar nanoparticles and Cd in saturated porous media was studied in cotransport experiments. Then, the effect of biochar nanoparticles on the release of Cd in contaminated media was explored by elution experiments. The cotransport experiments showed that the mobility of Cd was enhanced by two kinds of biochar nanoparticles, while the transport of biochar was limited due to the presence of Cd. The elution experiments showed that the transport of biochar nanoparticles can be inhibited by Cd previously immobilized in the sand column, and Cd can be rereleased by biochar nanoparticles. The rerelease ability of nZVI-NBC to Cd is weaker than that of NBC because nZVI is more easily retained in the sand column after oxidation, thus strengthening the immobilization of Cd. In general, the recoveries of NBC, nZVI-NBC and Cd in saturated porous media were reduced by the presence of clay minerals. The experimental results describing the stability of biochar nanoparticles in sand columns are consistent with those predicted by the Derjaguin-Landau-Verwey-Overbeek theory. The transport behavior of Cd and biochar nanoparticles in sand columns can be well simulated by advection-dispersion-reaction. These findings reveal the interaction between biochar nanoparticles and heavy metals in the soil environment and provide new insights into the transport and fate of environmental remediation materials and pollutants in the underground environment.
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http://dx.doi.org/10.1016/j.ecoenv.2021.112971DOI Listing
November 2021

Application of Multivariate Methods to Evaluate Differential Material Attributes of HPMC from Different Sources.

ACS Omega 2021 Nov 21;6(43):28598-28610. Epub 2021 Oct 21.

Key Laboratory of Biorheological Science and Technology, Ministry of Education, College of Bioengineering, Chongqing University, Chongqing 400030, China.

The aim of the present study is to achieve differential material attributes (DMAs) of hydroxypropyl methylcellulose (HPMC) with different viscosity grades (K4M, K15M, and K100M) from different manufacturers (Anhui Shanhe and Dow Chemical). Two kinds of multivariate methods, principal component analysis (PCA) and orthogonal partial least squares discriminant analysis (OPLS-DA), were adopted. The physicochemical properties of HPMC were systematically investigated via various techniques (e.g., SEM, particle size detection, and SeDeM characterization). Data from 33 characterization variables were applied to the multivariate methods. The PCA and OPLS-DA results indicated the differences between the HPMC from two manufacturers by the common variables that include the tablet hardness (HD), tensile strength (TS), bulk density, interparticle porosity, Carr index, cohesion index, Hausner ratio, flowability, and the width of the particle size distribution (span). Interestingly, these variables showed a certain correlation with each other, supporting the characterization results. Except for these different variables of the HPMC obtained by multivariate analysis results, distinguishable shapes and surface morphologies also appeared between different sources. To sum up, the powder properties (particle size, surface topography, dimension, flowability, and compressibility) and the tablet properties (HD and TS) were recognized as the DMAs of HPMC samples. This work provided the multivariate methods for the physicochemical characterization of HPMC, with potential in the quality control and formulation development.
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http://dx.doi.org/10.1021/acsomega.1c03009DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8567258PMC
November 2021

Purification, identification and molecular mechanism of dipeptidyl peptidase IV inhibitory peptides from discarded shrimp (Penaeus vannamei) head.

J Chromatogr B Analyt Technol Biomed Life Sci 2021 Dec 28;1186:122990. Epub 2021 Oct 28.

College of Food Science and Technology, Shanghai Ocean University, Shanghai 201306, China; National R&D Branch Center for Freshwater Aquatic Products Processing Technology (Shanghai), Shanghai 201306, China. Electronic address:

DPP-IV plays a key role for regulation of glucose metabolism in the body. The object of this study was to obtain DPP-IV inhibitors from discarded but protein-rich Penaeus vannamei (P. vannamei) head, and to explore the potential mechanism between DPP-IV and its inhibitors. P. vannamei head protein was hydrolyzed by five food grade proteases, respectively. The animal protease hydrolysate showed the highest inhibitory active. Then the hydrolysate was sequentially separated by ultrafiltration, gel filtration chromatography and reversed phase high-performance liquid chromatography (RP-HPLC), the peptides sequences were identified by LC-MS/MS and four potential peptides YPGE, VPW, HPLY, YATP showed superior DPP-IV inhibitory activity. Meanwhile, molecular docking effectively explored their mechanism through formed hydrogen bonds and hydrophobic regions. The four peptides showed better DPP-IV inhibitory activity stability with heating treatment, pH (1-10) treatment, and in vitro gastrointestinal digestion. Our results demonstrated that the protein hydrolysate from discarded P. vannamei head can be considered as a promising natural source of DPP-IV inhibitor for helping to improve glycaemic control in Type 2 diabetes.
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http://dx.doi.org/10.1016/j.jchromb.2021.122990DOI Listing
December 2021

Dysregulated Microbiota-Driven Gasdermin D Activation Promotes Colitis Development by Mediating IL-18 Release.

Front Immunol 2021 14;12:750841. Epub 2021 Oct 14.

Department of Medical Laboratory, Shenzhen Longhua District Central Hospital, Affiliated Central Hospital of Shenzhen Longhua District, Guangdong Medical University, Shenzhen, China.

The balance between gut microbiota and host is critical for maintaining host health. Although dysregulation of the gut microbiota triggers the development of various inflammatory diseases, including colitis, the molecular mechanism of microbiota-driven colitis development is largely unknown. Here, we found that gasdermin D (GSDMD) was activated during acute colitis. In the dextran sulfate sodium (DSS)-induced colitis model, compared to wild-type mice, -deficient mice had less colitis severity. Mechanistically, GSDMD expression in intestinal epithelial cells (IECs), but not infiltrating immune cells, was critical for GSDMD-mediated colitis progression. Moreover, commensal () largely overgrew during colitis, and then the dysregulated commensal mediated GSDMD activation. Furthermore, the activated GSDMD promoted the release of interleukin-18 (IL-18), but not the transcript or maturation level of IL-18, which in turn mediated goblet cell loss to induce colitis development. Thus, GSDMD promotes colitis development by mediating IL-18 release, and the microbiota can mediate colitis pathogenesis through regulation of GSDMD activation. Our results provide a potential molecular mechanism by which the microbiota-driven GSDMD activation contributes to colitis pathogenesis.
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http://dx.doi.org/10.3389/fimmu.2021.750841DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8551709PMC
January 2022

Atractyloside Protect Mice Against Liver Steatosis by Activation of Autophagy ANT-AMPK-mTORC1 Signaling Pathway.

Front Pharmacol 2021 21;12:736655. Epub 2021 Sep 21.

Department of Medical Laboratory, Shenzhen Longhua District Central Hospital, The Affiliated Central Hospital of Shenzhen Longhua District, Guangdong Medical University, Shenzhen, China.

: Adenine nucleotide translocase (ANT) can transport ADP from cytoplasm to mitochondrial matrix and provide raw materials for ATP synthesis by oxidative phosphorylation. Dysfunction of ANT leads to limitation of ADP transport and decrease of ATP production. Atractyloside (ATR) is considered as a cytotoxic competitive inhibitor binding to ANT, making ANT vulnerable to transport ADP, and reduces ATP synthesis. Moreover, the blockage of ANT by ATR may increase ADP/ATP ratio, activate AMPK-mTORC1-autophagy signaling pathway, and promote lipid degradation in steatosis hepatocytes. The present study was conducted to investigate the mechanism of ATR, regulate ANT-AMPK-mTORC1 signaling pathway to activate autophagy, and promote the degradation of lipid droplets in high-fat diet (HFD) induced liver steatosis. : ICR mice were fed with HFD for 8 weeks to induce liver steatosis, and ATR solution was given by intraperitoneal injection. Intracellular triglyceride level and oil red O staining-lipid droplets (LDs) were assessed, the expression of proteins related to ANT-AMPK-mTORC1 signaling pathway and autophagy were determined, and the colocalization of LC3B and Perilipin 2 was performed. : ATR treatment decreased the serum AST level, relative weight of liver and epididymal fat, and body weight of HFD mice. The LDs in HFD mice livers were reduced in the presence of ATR, and the TG level in serum and liver of HFD mice was significantly reduced by ATR. In addition, ATR inhibited ANT2 expression, promoted the activation of AMPK, then increased Raptor expression, and finally decreased the mTOR activity. Furthermore, ATR increased the protein level of LC3A/B and ATG7, and a strong colocalization of LC3B and PLIN2 was observed. : ATR treatment blocks ANT2 expression, promotes the activation of AMPK, then decreases the mTOR activity, and finally promotes autophagosomes formation, thus accelerating the degradation of HFD-induced accumulated lipids in the liver. This will provide new therapeutic ideas and experimental data for clinical prevention and treatment of non-alcoholic fatty liver disease.
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http://dx.doi.org/10.3389/fphar.2021.736655DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8490973PMC
September 2021

Second-victim experience and support among nurses in mainland China.

J Nurs Manag 2022 Jan 29;30(1):260-267. Epub 2021 Oct 29.

Operating room, Guizhou Medical University school of Stomatology, Guiyang, China.

Aims: To investigate the experience and support of nurses as second victims in adverse events and explore factors.

Background: Adverse events have significant negative influences on healthcare professionals. However, there is still a lack of research on the impacts of individual and event factors.

Methods: A cross-sectional survey via a self-report electronic questionnaire was sent to approximately 6400 nurses from six tertiary comprehensive hospitals in mainland China. The socio-demographic, adverse event-related information and second-victim experience and support questionnaires were used.

Results: Approximately 2897 (45.26%) of nurses were involved in at least one adverse event that mainly caused psychological distress. Male nurses, Grade I adverse events, public reports and discussions of adverse events were related to the second-victim reaction. Discussing with a respected colleague was the most strongly desired form of support.

Conclusions: Chinese nurses have reported a high prevalence of psychological reactions. Follow-up studies must consider other potential factors.

Implications For Nursing Management: Nursing managers should consider the factors that affect second victims and provide support based on the expectations of nurses. Psychological first-aid and immediate and mid- to long-term support strategies should be provided to help the second victim alleviate distress.
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http://dx.doi.org/10.1111/jonm.13490DOI Listing
January 2022

Association between Hypertriglyceridemic-Waist Phenotype and Risk of Type 2 Diabetes Mellitus in Middle-Aged and Older Chinese Population: A Longitudinal Cohort Study.

Int J Environ Res Public Health 2021 09 12;18(18). Epub 2021 Sep 12.

School of Public Health, Sun Yat-Sen University, 74 Zhongshan Second Road, Guangzhou 510080, China.

Current evidence remains inconsistent with regard to the association between different triglyceridemic-waist phenotypes and the risks for type 2 diabetes mellitus (T2DM). We aimed to investigate this association among a retrospective cohort analysis of 6918 participants aged ≥ 45 years in the China Health and Retirement Longitudinal Study (CHARLS). Participants were categorized into four triglyceridemic-waist phenotypes consisting of NWNT (normal waist circumference and normal triglycerides), NWHT (normal waist circumference and high triglycerides), EWNT (enlarged waist circumference and normal triglycerides), and EWHT (enlarged waist circumference and high triglycerides) based on participants' baseline information. Multivariate log-binomial regression was used to assess the T2DM risk in different phenotypes. Subgroup analysis was conducted to test the robustness of the findings. After 4-years of follow-up, participants with EWHT (Relative Risk [RR]: 1.909, 95% Confidence Interval [CI]: 1.499 to 2.447) or EWNT (RR: 1.580, 95%CI: 1.265 to 1.972) phenotypes had significantly higher likelihood of incident T2DM compared to the NWNT phenotype, whereas the association was not significant for the NWHT phenotype (RR: 1.063, 95%CI: 0.793 to 1.425). The subgroup analyses generally revealed similar associations across all subgroups. Among middle-aged and older adults, we suggested a combined use of waist circumference and triglycerides measures in identifying participants who are at high risk of developing T2DM.
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http://dx.doi.org/10.3390/ijerph18189618DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8466002PMC
September 2021

A hydrophilic-hydrophobic graphitic carbon [email protected] hybrid substrate for recyclable surface-enhanced Raman scattering-based detection without the coffee-ring effect.

Analyst 2021 Sep 27;146(19):5923-5933. Epub 2021 Sep 27.

Department of Microelectronic Science and Engineering, School of Physical Science and Technology, Ningbo University, Ningbo 315211, Zhejiang, P. R. China.

There is growing interest in developing a multifunctional surface-enhanced Raman scattering (SERS) substrate to deal with the challenge in the pretreatment-free detection and degradation of hazardous organic pollutants in water. Herein, a hydrophilic-hydrophobic graphitic carbon [email protected] ([email protected]) hybrid substrate was exploited as a potential candidate for the recyclable detection of dye molecules. Such a sophisticated substrate not only showed a significant SERS activity with a high enhancement factor of 3.21 × 10 triggered by the significantly aggregated Ag nanoparticles, but also possessed an outstanding self-cleaning property visible-light irradiation. Furthermore, the effective weakening of the coffee-ring effect was also facilitated by the hydrophilic-hydrophobic structure, resulting in excellent uniformity and reproducibility. Ultimately, the applicability of the developed recyclable SERS substrate in the monitoring of trace malachite green was demonstrated. It is expected that the innovative SERS substrate has great potential for application in highly sensitive, stable, and recyclable on-site analysis, especially for organic pollutant treatment and environmental protection.
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http://dx.doi.org/10.1039/d1an01121aDOI Listing
September 2021

Metal-organic frameworks for advanced drug delivery.

Acta Pharm Sin B 2021 Aug 13;11(8):2362-2395. Epub 2021 Mar 13.

Center for Drug Delivery Systems, Shanghai Institute of Materia Medica, Chinese Academy of Sciences, Shanghai 201203, China.

Metal-organic frameworks (MOFs), comprised of organic ligands and metal ions/metal clusters coordinative bonds are highly porous, crystalline materials. Their tunable porosity, chemical composition, size and shape, and easy surface functionalization make this large family more and more popular for drug delivery. There is a growing interest over the last decades in the design of engineered MOFs with controlled sizes for a variety of biomedical applications. This article presents an overall review and perspectives of MOFs-based drug delivery systems (DDSs), starting with the MOFs classification adapted for DDSs based on the types of constituting metals and ligands. Then, the synthesis and characterization of MOFs for DDSs are developed, followed by the drug loading strategies, applications, biopharmaceutics and quality control. Importantly, a variety of representative applications of MOFs are detailed from a point of view of applications in pharmaceutics, diseases therapy and advanced DDSs. In particular, the biopharmaceutics and quality control of MOFs-based DDSs are summarized with critical issues to be addressed. Finally, challenges in MOFs development for DDSs are discussed, such as biostability, biosafety, biopharmaceutics and nomenclature.
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http://dx.doi.org/10.1016/j.apsb.2021.03.019DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8424373PMC
August 2021

The association of nighttime sleep duration and quality with chronic kidney disease in middle-aged and older Chinese: a cohort study.

Sleep Med 2021 10 13;86:25-31. Epub 2021 Aug 13.

School of Public Health, Sun Yat-sen University, Guangzhou, China. Electronic address:

Objective: This cohort study aimed to assess the associations between sleep duration and quality with the risk of incident chronic kidney disease (CKD) in middle-aged and older Chinese.

Methods: We used the 2011 and 2015 surveys of the China Health and Retirement Longitudinal Study (CHARLS). Nighttime sleep duration was categorized into five groups: ≤4, (4-6], (6-8], (8-10], and >10 h/night. Sleep quality was assessed by restless days in the past week (<1, 1-2, 3-4, and 5-7 days/week). Multivariate logistic regression was used to assess the association between sleep duration and quality with incident CKD.

Results: A total of 11,339 participants free of CKD at baseline were included in this study. After four years follow-up, the incidence of CKD was 7.8%. There was a "U-shaped" association between sleep duration and risk of CKD. Compared to 6-8 h of nighttime sleep duration, those who slept ≤4 h/night (RR: 1.639, 95% CI: 1.287-2.087) or >10 h/night (RR: 2.342, 95% CI: 1.007-5.451) had increased risk of developing CKD after adjustment for confounders. Participants with 5-7 restless days per week had significantly increased risk of CKD (adjusted RR: 1.686, 95% CI: 1.352-2.102), compared to those who rarely or never had a restless sleep.

Conclusions: Extreme nighttime sleep duration and poor sleep quality were associated with increased risk of CKD in middle-aged and older Chinese. Obtaining an optimal nighttime sleep duration and better sleep quality might reduce the risk of CKD.
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http://dx.doi.org/10.1016/j.sleep.2021.08.007DOI Listing
October 2021

Corrigendum: TKI-Resistant Renal Cancer Secretes Low-Level Exosomal miR-549a to Induce Vascular Permeability and Angiogenesis to Promote Tumor Metastasis.

Front Cell Dev Biol 2021 19;9:726535. Epub 2021 Jul 19.

Department of Urology Surgery, Xiang'an Hospital, Xiamen University, Xiamen, China.

[This corrects the article DOI: 10.3389/fcell.2021.689947.].
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http://dx.doi.org/10.3389/fcell.2021.726535DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8327907PMC
July 2021

Autophagy in Xp11 translocation renal cell carcinoma: from bench to bedside.

Mol Cell Biochem 2021 Dec 3;476(12):4231-4244. Epub 2021 Aug 3.

Department of Medical Laboratory, Shenzhen Longhua District Central Hospital, Guangdong Medical University, Shenzhen, 518110, China.

Xp11 translocation renal cell carcinoma (tRCC) characterized by the rearrangement of the TFE3 is recently identified as a unique subtype of RCC that urgently requires effective prevention and treatment strategies. Therefore, determining suitable therapeutic targets and fully understanding the biological significance of tRCC is essential. The importance of autophagy is increasingly acknowledged because it shows carcinogenic activity or suppressor effect. Autophagy is a physiological cellular process critical to maintaining cell homeostasis, which is involved in the lysosomal degradation of cytoplasmic organelles and macromolecules via the lysosomal pathway, suggesting that targeting autophagy is a potential therapeutic approach for cancer therapies. However, the underlying mechanism of autophagy in tRCC is still ambiguous. In this review, we summarize the autophagy-related signaling pathways associated with tRCC. Moreover, we examine the roles of autophagy and the immune response in tumorigenesis and investigate how these factors interact to facilitate or prevent tumorigenesis. Besides, we review the findings regarding the treatment of tRCC via induction or inhibition of autophagy. Hopefully, this study will shed some light on the functions and implications of autophagy and emphasize its role as a potential molecular target for therapeutic intervention in tRCC.
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http://dx.doi.org/10.1007/s11010-021-04235-wDOI Listing
December 2021

PharmDE: A new expert system for drug-excipient compatibility evaluation.

Int J Pharm 2021 Sep 31;607:120962. Epub 2021 Jul 31.

State Key Laboratory of Quality Research in Chinese Medicine, Institute of Chinese Medical Sciences (ICMS), University of Macau, Macau, China. Electronic address:

Drug-excipient compatibility study is the essential basis for excipient selection at the pre-formulation stage. According to the pharmaceutical Quality by Design (QbD) principles, a comprehensive understanding of the ingredients' physicochemical properties and a theoretical evaluation of the interaction risk between the drugs and excipients are required for conducting rational compatibility experimental design. Currently, there is an urgent need to establish an artificial intelligence system for researchers to easily get through the problem because it is very inconvenient and hard to utilize those drug-excipient incompatibility data scattered in scientific literature. Here, we designed a knowledge-driven expert system named PharmDE for drug-excipient incompatibility risk evaluation. PharmDE firstly developed an information-rich database to store incompatibility data, covering 532 data items from 228 selected articles. Then, 60 drug-excipient interaction rules were created based on our knowledge and formulation research experiences. Finally, the expert system was developed by organically integrating the database searching and rule-based incompatibility risk prediction, which resulted in four main functionalities: basic search of incompatibility database, data matching by similarity search, drug incompatibility risk evaluation, and formulation incompatibility risk evaluation. PharmDE is expected to be a useful tool for drug-excipient compatibility study and accelerate drug formulation design. It is now freely available at https://pharmde.computpharm.org.
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http://dx.doi.org/10.1016/j.ijpharm.2021.120962DOI Listing
September 2021

Abnormal interhemispheric resting state functional connectivity in Parkinson's disease patients with impulse control disorders.

NPJ Parkinsons Dis 2021 Jul 16;7(1):60. Epub 2021 Jul 16.

Department of Neurology, The First Affiliated Hospital of Nanjing Medical University, Nanjing, China.

Impulse control disorders (ICD) in Parkinson's disease (PD) might be attributed to misestimate of rewards or the failure to curb inappropriate choices. The mechanisms underlying ICD were reported to involve the lateralization of monoamine network. Our objective was to probe the significant role of lateralization in the pathogenesis of ICD. Twenty-one PD patients with ICD (PD-ICD), thirty-three without ICD (PD-no ICD), and thirty-seven healthy controls (HCs) were recruited and performed T1-weighted, diffusion tensor imaging (DTI) scans and resting state functional magnetic resonance imaging (rs-fMRI). By applying the Voxel-mirrored Homotopic Connectivity (VMHC) and Freesurfer, we evaluated participants' synchronicity of functional connectivity and structural changes between hemispheres. Also, tract-based spatial statistics (TBSS) was applied to compare fiber tracts differences. Relative to PD-no ICD group, PD-ICD group demonstrated reduced VMHC values in middle frontal gyrus (MFG). Compared to HCs, PD-ICD group mainly showed decreased VMHC values in MFG, middle and superior orbital frontal gyrus (OFG), inferior frontal gyrus (IFG) and caudate, which were related to reward processing and inhibitory control. The severity of impulsivity was negatively correlated with the mean VMHC values of MFG in PD-ICD group. Receiver operating characteristic (ROC) curves analyses uncovered that the mean VMHC values of MFG might be a potential marker identifying PD-ICD patients. However, we found no corresponding asymmetrical alteration in cortical thickness and no significant differences in fractional anisotropy (FA) and mean diffusivity (MD). Our results provided further evidence for asymmetry of functional connectivity in mesolimbic reward and response inhibition network in ICD.
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http://dx.doi.org/10.1038/s41531-021-00205-7DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8285494PMC
July 2021

TKI-Resistant Renal Cancer Secretes Low-Level Exosomal miR-549a to Induce Vascular Permeability and Angiogenesis to Promote Tumor Metastasis.

Front Cell Dev Biol 2021 10;9:689947. Epub 2021 Jun 10.

Department of Urology Surgery, Xiang'an Hospital, Xiamen University, Xiamen, China.

Tyrosine kinase inhibitors (TKI)-resistant renal cancer is highly susceptible to metastasis, and enhanced vascular permeability promotes the process of metastasis. To evaluate the effect of cancer-derived exosomes on vascular endothelial cells and clarify the mechanism of metastasis in TKI-resistant renal cancer, we studied the crosstalk between clear cell renal cell carcinoma (ccRCC) cells and human umbilical vein endothelial cells (HUVECs). Exosomes from ccRCC cells enhanced the expression of vascular permeability-related proteins. Compared with sensitive strains, exosomes from resistant strains significantly enhanced vascular endothelial permeability, induced tumor angiogenesis and enhanced tumor lung metastasis in nude mice. The expression of miR-549a is lower in TKI-resistant cells and exosomes, which enhanced the expression of HIF1α in endothelial cells. In addition, TKI-resistant RCC cells reduced nuclear output of pre-miR-549a via the VEGFR2-ERK-XPO5 pathway, and reduced enrichment of mature miR-549a in cytoplasm, which in turn promoted HIF1α expression in RCC, leading to increased VEGF secretion and further activated VEGFR2 to form a feedback effect. miR-549a played an important role in the metastasis of renal cancer and might serve as a blood biomarker for ccRCC metastasis and even had the potential of becoming a new drug to inhibit TKI-resistance.
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http://dx.doi.org/10.3389/fcell.2021.689947DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8222687PMC
June 2021

Analysis of RNA mA methylation regulators and tumour immune cell infiltration characterization in prostate cancer.

Artif Cells Nanomed Biotechnol 2021 Dec;49(1):407-435

Department of Urology, School of Medicine, Xiang'an Hospital of Xiamen University, Xiamen University, Xiamen, China.

Potential roles of RNA N6-methyladenosine (mA) modification in tumour microenvironment (TME) cell infiltration has been demonstrated in recent studies. Nonetheless, the mechanism of its regulation remains unknown and immunotherapy has been marginal in prostate cancer. We demonstrated the expression of different mA regulators within prostate cancer related to genetic variation, alternative splicing (AS), tumour mutational burden (TMB) and TME. Unsupervised clustering and risk prediction model constructed by 24 mA regulators could predict scores of TME and prostate cancer patients prognosis. T cells CD8 was the intersection of immune cells which are related to multiple biological processes, and the fraction of T cells CD8 strongly correlates with immune associated gene sets. mA methylation modification and immune cells infiltration played a nonnegligible role in prostate cancer. Our study represents a step towards personalized immunotherapy for prostate cancer patients.
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http://dx.doi.org/10.1080/21691401.2021.1912759DOI Listing
December 2021

Ferroptosis: Biochemistry and Biology in Cancers.

Front Oncol 2021 1;11:579286. Epub 2021 Apr 1.

Department of Urology, Xiang'an Hospital of Xiamen University, Xiamen, China.

The challenge of eradicating cancer is that cancer cells possess diverse mechanisms to protect themselves from clinical strategies. Recently, ferroptosis has been shown to exhibit appreciable anti-tumor activity that could be harnessed for cancer therapy in the future. Ferroptosis is an iron-dependent form of regulated cell death that is characterized by the oxidization of polyunsaturated fatty acids (PUFAs) and accumulation of lipid peroxides. Ferroptosis has been closely correlated with numerous biological processes, such as amino acid metabolism, glutathione metabolism, iron metabolism, and lipid metabolism, as well as key regulators including GPX4, FSP1, NRF2, and p53. Although ferroptosis could be involved in killing various cancer cells, multiple aspects of this phenomenon remain unresolved. In this review, we summarize the biochemistry and biology of ferroptosis in diverse cancers and discuss the potential mechanisms of ferroptosis, which might pave the way for guiding cancer therapeutics.
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http://dx.doi.org/10.3389/fonc.2021.579286DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8047310PMC
April 2021

Anti-Adhesion Effects of Lactobacillus Strains on Caco-2 Cells Against Escherichia Coli and Their Application in Ameliorating the Symptoms of Dextran Sulfate Sodium-Induced Colitis in Mice.

Probiotics Antimicrob Proteins 2021 12 14;13(6):1632-1643. Epub 2021 Apr 14.

College of Food Science and Engineering, Northwest A&F University, Yangling, 712100, Shaanxi Province, China.

The beneficial effects of probiotics on ameliorating ulcerative colitis (UC) have attracted much attention in recent years. Nevertheless, the number of these identified probiotics is still limited. In addition, the adhesion abilities of probiotics are considered to be a key determinant for probiotic efficacy. However, the relationship between the adhesion abilities of probiotics and their role in ameliorating UC has been poorly studied to date. This study measured the adhesion abilities of four Lactobacillus strains to Caco-2 cells and their anti-adhesion effects on Caco-2 cells against pathogenic bacteria, as well as their application in ameliorating the symptoms of dextran sulfate sodium-induced UC, and further illustrated the relationship between these two potential probiotic properties of probiotics and their beneficial effects on UC. Results suggested that the adhesion abilities of the four tested Lactobacillus strains exists highly strain-specific and the mechanisms of their anti-adhesion effect on Caco-2 cells against Escherichia coli may be different. Moreover, all these strains had promising effects on ameliorating UC by reducing inflammatory response and improving the intestinal mucosal barrier function, as well as promoting the production of SCFAs. In conclusion, the four tested Lactobacillus strains can be considered as alternative dietary supplements in alleviating UC. In addition, it could be concluded that there is no significant correlation between the adhesion abilities of probiotics and their role in ameliorating UC, which further illustrated that the adhesion properties of probiotics in vitro may not be suitable as the key criterion for screening potential strains with UC-alleviating effects.
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http://dx.doi.org/10.1007/s12602-021-09774-8DOI Listing
December 2021

Advances in polysaccharide nanocrystals as pharmaceutical excipients.

Carbohydr Polym 2021 Jun 8;262:117922. Epub 2021 Mar 8.

CAS Key Laboratory for Biomedical Effects of Nanomaterials and Nanosafety, CAS Center for Excellence in Nanoscience, National Center for NanoScience and Technology, No. 11 Zhongguancun Beiyitiao, Haidian District, Beijing, 100190, PR China; NMPA Key Laboratory for Quality Research and Evaluation of Pharmaceutical Excipients, National Institutes for Food and Drug Control, 2 Tiantan Xi Li, Beijing, 100050, PR China. Electronic address:

Polysaccharide nanocrystals (PNs) are attractive pharmaceutical excipients due to their abundant surface hydroxyl groups, high surface charges, prominent mechanical properties, excellent fluidity, and good swelling properties. In this review, we summarize three kinds of PNs, including cellulose nanocrystals (CNCs), starch nanocrystals (SNCs), and chitin nanocrystals (ChNCs). We introduce the applications of PNs as stabilizers, adsorbents, film-forming materials, gel materials, disintegrants, and ointment matrices. We focus on the advantages of PNs to improve mechanical properties, thermal stability, therapeutic effect, biocompatibility, and release of active pharmaceutical ingredients. We discuss regulatory issues of PNs. We finally propose the challenges and future perspectives of PNs as pharmaceutical excipients.
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http://dx.doi.org/10.1016/j.carbpol.2021.117922DOI Listing
June 2021

Co-transport of ball-milled biochar and Cd in saturated porous media.

J Hazard Mater 2021 08 23;416:125725. Epub 2021 Mar 23.

Agro-Environmental Protection Institute, Agro-Environmental Protection Institute, Ministry of Agriculture and Rural Affairs of the People's Republic of China, Tianjin 300191, China.

The combination of ball milling technology and biochar materials provides new prospects for environmentally friendly and sustainable environmental pollution control technologies, but comes with opportunities and risks. In this study, column experiments were used to evaluate the environmental behavior of ball‑milled biochar (BMBC). The results of the column experiments showed that BMBC transport increased with a high flow velocity, large medium size, high pH, and low ionic strength. Owing to the strong adsorption of Cd by BMBC, the presence of BMBC in the medium led to a decrease in effluent Cd. The presence of Cd in the solution slightly inhibited the transport of BMBC. The transport of Cd was facilitated by BMBC due to the high affinity. Therefore, attention should be paid to favorable conditions for BMBC transport. This study provides a perspective to assess the behavior of BMBC in the environment and whether its interaction with Cd will introduce new environmental hazards.
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http://dx.doi.org/10.1016/j.jhazmat.2021.125725DOI Listing
August 2021

Effect of surfactants on the transport of polyethylene and polypropylene microplastics in porous media.

Water Res 2021 May 8;196:117016. Epub 2021 Mar 8.

Agro-Environmental Protection Institute, Agro-Environmental Protection Institute, Ministry of Agriculture and Rural Affairs of the People's Republic of China Tianjin, China 300191.

The transport of microplastics in porous media is attracting increasing attention. However, to date, research is limited to polystyrene microplastics. Meanwhile, surfactants can promote solid dispersion to form a stable suspension, possibly allowing microplastics to migrate when attached to a surfactant, which would increase the scope and degree of microplastic pollution, further endangering human health and the stability of the ecological environment. Therefore, in this study, the transport behavior of microplastics in porous media was explored in the presence of surfactants. Herein, polyethylene (PE) and polypropylene (PP) were evaluated while dispersed by two ionic surfactants: cationic surfactant-cetyltrimethylammonium bromide (CTAB) and anionic surfactant-sodium dodecylbenzenesulfonate (SDBS). The influence of different factors (surfactant concentration, ionic strength, pH, flow rate, and multivalent cations) on the transport of microplastics in porous media was explored via quartz sand packed-column experiments. Our experimental results show that the transport abilities of PE and PP increased with increasing surfactant concentration when the surfactant concentration was less than the critical micelle concentration (CMC). In the presence of CTAB and SDBS, physicochemical factors had different effects on the transport of microplastics mainly by controlling Zeta potential, advection diffusion and CMC. The mobility of PE and PP decreased with increasing ionic strength, cation valence and pH, and decreasing flow rate. However, the mobility of PE and PP under CTAB is much greater than that of PE and PP under SDBS, because quartz sand can absorb more CTAB molecules through electrostatic attraction to weaken the collision between microplastics and quartz sand. Further, the transport ability of PP was greater than that of PE under all conditions considered. Notably, the Extended-Derjaguin-Landau-Verwey-Overbeek (XDLVO) theory formed by adding osmotic, elastic, and hydrophobic force could well described the migration behavior of microplastics in CTAB and SDBS well. This research highlights that surfactant has a significant impact on the transport ability of microplastics, and provides a comprehensive understanding of the migration and fate behaviors of microplastics affected by surfactants, which is necessary to prevent and reduce the environmental hazards of microplastics.
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http://dx.doi.org/10.1016/j.watres.2021.117016DOI Listing
May 2021

[Biodegradation of furan aldehydes in lignocellulose hydrolysates].

Sheng Wu Gong Cheng Xue Bao 2021 Feb;37(2):473-485

College of Chemical Engineering, Nanjing Forestry University, Nanjing 210037, Jiangsu, China.

Lignocellulose is the most abundant renewable organic carbon resource on earth. However, due to its complex structure, it must undergo a series of pretreatment processes before it can be efficiently utilized by microorganisms. The pretreatment process inevitably generates typical inhibitors such as furan aldehydes that seriously hinder the growth of microorganisms and the subsequent fermentation process. It is an important research field for bio-refining to recognize and clarify the furan aldehydes metabolic pathway of microorganisms and further develop microbial strains with strong tolerance and transformation ability towards these inhibitors. This article reviews the sources of furan aldehyde inhibitors, the inhibition mechanism of furan aldehydes on microorganisms, the furan aldehydes degradation pathways in microorganisms, and particularly focuses on the research progress of using biotechnological strategies to degrade furan aldehyde inhibitors. The main technical methods include traditional adaptive evolution engineering and metabolic engineering, and the emerging microbial co-cultivation systems as well as functional materials assisted microorganisms to remove furan aldehydes.
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http://dx.doi.org/10.13345/j.cjb.200341DOI Listing
February 2021

Effects of Invasion on Soil Microbial Community Structure and Ecological Functions.

Microorganisms 2021 Jan 9;9(1). Epub 2021 Jan 9.

Collaborative Innovation Center of Sustainable Forestry in Southern China of Jiangsu Province, Nanjing Forestry University, Nanjing 210037, China.

It has been reported that the invasion of changed the soil microbial community in the mangrove ecosystem in China, especially the bacterial community, although the response of soil fungal communities and soil microbial ecological functions to the invasion of remains unclear. In this study, we selected three different communities (i.e., community (SC), -mangrove mixed community (TC), and mangrove community (MC)) in the Zhangjiangkou Mangrove Nature Reserve in China. High-throughput sequencing technology was used to analyze the impact of invasion on mangrove soil microbial communities. Our results indicate that the invasion of does not cause significant changes in microbial diversity, but it can alter the community structure of soil bacteria. The results of the LEfSe (LDA Effect Size) analysis show that the relative abundance of some bacterial taxa is not significantly different between the MC and SC communities, but different changes have occurred during the invasion process (i.e., TC community). Different from the results of the bacterial community, the invasion of only cause a significant increase in few fungal taxa during the invasion process, and these taxa are at some lower levels (such as family, genus, and species) and classified into the phylum . Although the invasion of changes the taxa with certain ecological functions, it may not change the potential ecological functions of soil microorganisms (i.e., the potential metabolic pathways of bacteria, nutritional patterns, and fungal associations). In general, the invasion of changes the community structure of soil microorganisms, but it may not affect the potential ecological functions of soil microorganisms.
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http://dx.doi.org/10.3390/microorganisms9010138DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7827921PMC
January 2021

Identification of predictors based on drug targets highlights accurate treatment of goserelin in breast and prostate cancer.

Cell Biosci 2021 Jan 6;11(1). Epub 2021 Jan 6.

Department of Surgery, The Second Affiliated Hospital of Harbin Medical University, Harbin, China.

Goserelin is an effective alternative to surgery or estrogen therapy in prostate cancer palliation, and possibly to ovariectomy in premenopausal breast cancer. However, not all users of goserelin can benefit from it, or some patients are not sensitive to goserelin. The advent of network pharmacology has highlighted the need for accurate treatment and predictive biomarkers. In this study, we successfully to identify 76 potential targets related to the compound of goserelin through network pharmacology approach. We also identified 18 DEGs in breast cancer tissues and 5 DEGs in cells, and 6 DEGs in prostate cancer tissues and 9 DEGs in cells. CRABP2 is the common DEG both in breast and prostate cancer. The risk prediction models constructed with potential prognostic targets of goserelin can successfully predict the prognosis in breast and prostate cancer, especially for very young breast cancer patients. Moreover, seven subgroups in breast cancer and six subgroups in prostate cancer were respectively identified based on consensus clustering using potential prognostic targets of goserelin that significantly influenced survival. The expression of representative genes including CORO1A and ANXA5 in breast and DPP4 in prostate showed strong correlations with clinic-pathological factors. Taken together, the novel signature can facilitate identification of new biomarkers which sensitive to goserelin, increase the using accuracy of goserelin and clarify the classification of disease molecular subtypes in breast and prostate cancer.
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http://dx.doi.org/10.1186/s13578-020-00517-wDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7788753PMC
January 2021
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