Publications by authors named "Huijun Cui"

9 Publications

  • Page 1 of 1

Effects of chitooligosaccharide-functionalized graphene oxide on stability, simulated digestion, and antioxidant activity of blueberry anthocyanins.

Food Chem 2021 Jul 22;368:130684. Epub 2021 Jul 22.

College of Food Science, Key Laboratory of Healthy Food Nutrition and Innovative Manufacturing of Liaoning Province, National R&D Professional Center for Berry Processing, Shenyang Agricultural University, Shenyang, Liaoning 110866, China; Department of Food Science, Cornell University, Ithaca, NY 14850-7201, United States. Electronic address:

In this study, we tested the in vitro efficacy of a graphene oxide-chitooligosaccharide (GO-COS) complex developed to protect blueberry anthocyanins (An) from degradation by various physicochemical factors and the digestive process. We prepared a GO-COS complex to adsorb An and protect them from the destructive effects of their ambient environment. The complex protected the An under various temperature, pH, light, oxidant, and reductant conditions. We evaluated An content and composition in a simulated digestive system using the pH differential method and the high performance liquid chromatography-mass spectrometry (HPLC-MS). The GO-COS carrier stabilized An in the intestine and protected their peroxyl radical-scavenging capacity. Additionally, we observed a dose-response relationship between An content and cellular antioxidant activity, and simultaneous improvement of An bioavailability when the An were encapsulated in the complex. The complex inhibited HepG2 cell proliferation at the tested dose range. This study provides valuable information for stability of An-rich products.
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http://dx.doi.org/10.1016/j.foodchem.2021.130684DOI Listing
July 2021

Ecotoxicological effects of DBPs on freshwater phytoplankton communities in co-culture systems.

J Hazard Mater 2021 Jul 17;421:126679. Epub 2021 Jul 17.

State Key Laboratory of Urban Water Resource and Environment of Harbin Institute of Technology, Shenzhen 518055, PR China. Electronic address:

Intensive disinfection of wastewater during the COVID-19 pandemic might elevate the generation of toxic disinfection byproducts (DBPs), which has triggered global concerns about their ecological risks to natural aquatic ecosystems. In this study, the toxicity of 17 DBPs typically present in wastewater effluents on three representative microalgae, including Scenedesmus sp. (Chlorophyta), Microcystis aeruginosa (Cyanophyta), and Cyclotella sp. (Bacillariophyta) was investigated. The sensitivities of the three microalgae to DBPs varied greatly from species to species, indicating that DBPs may change the structure of phytoplankton communities. Later, co-cultures of these phytoplankton groups as a proxy of ecological freshwater scenario were conducted to explore the impacts of DBPs on phytoplankton community succession. M. aeruginosa became surprisingly dominant in co-cultures, representing over 50% after dosing with monochloroacetic acid (MCAA, 0.1-10 mg/L). The highest proportion of M. aeruginosa was 70.3% when exposed to 2 mg/L MCAA. Although Scenedesmus sp. dominated in monochloroacetonitrile (MCAN) exposure, M. aeruginosa accounted for no less than 30% even at 40 mg/L MCAN. In this study, DBPs disrupted the original inter-algal relationship in favor of M. aeruginosa, suggesting that DBPs may contribute to the outbreak of cyanobacterial blooms in aquatic ecosystems.
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http://dx.doi.org/10.1016/j.jhazmat.2021.126679DOI Listing
July 2021

Toxicity of 17 Disinfection By-products to Different Trophic Levels of Aquatic Organisms: Ecological Risks and Mechanisms.

Environ Sci Technol 2021 08 16;55(15):10534-10541. Epub 2021 Jun 16.

Shenzhen International Graduate School, Tsinghua University, Shenzhen 518055, P. R. China.

Intensified disinfection of wastewater during the COVID-19 pandemic increased the release of toxic disinfection by-products (DBPs). However, studies relating to the ecological impacts of DBPs on the aquatic environment remain insufficient. In this study, we comparatively investigated the toxicities and ecological risks of 17 typical, halogenated DBPs to three trophic levels of organisms in the freshwater ecosystem, including phytoplankton ( sp.), zooplankton (), and fish (). Toxicity of DBPs was found to be species-specific: sp. was the most sensitive to haloacetic acids, while was the most sensitive to haloacetonitriles and trihalomethanes. Specific to each DBP, toxicities were also related to their classes and substituted halogen atoms. Damage to photosystems and oxidative stress served as the potential mechanisms for DBPs toxicity to microalgae. The different sensitivities to DBPs indicate that a battery of bioassays with organisms at different trophic levels is necessary to determine the ecotoxicity of DBPs. Furthermore, the ecological risks of DBPs were assessed by calculating the risk quotients (RQs) based on toxicity data from multiple bioassays. The cumulative RQs of DBPs to all the organisms were greater than 1.0, indicating high ecological risks of DBPs in wastewater effluents.
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http://dx.doi.org/10.1021/acs.est.0c08796DOI Listing
August 2021

Effect of Blueberry Anthocyanin-Rich Extracts on Peripheral and Hippocampal Antioxidant Defensiveness: The Analysis of the Serum Fatty Acid Species and Gut Microbiota Profile.

J Agric Food Chem 2021 Mar 12;69(12):3658-3666. Epub 2021 Mar 12.

College of Food Science, Shenyang Agricultural University, Shenyang 110866, China.

The current study investigated the positive effects of blueberry anthocyanin-rich extracts (BAE) on either peripheral or hippocampal antioxidant defensiveness and established the connection of the improved antioxidant status with the altered fatty acid species and gut microbiota profile. High-fat diet-induced oxidative stress in C57BL/6 mice was attenuated by BAE administration, which was reflected by strengthened antioxidant enzymes, alleviated hepatic steatosis, and improved hippocampal neuronal status. Serum lipidomics analysis indicated that the fatty acid species were altered toward the elevated unsaturated/saturated ratio, along with phospholipid species toward enriched -3 polyunsaturated fatty acid compositions. The modulated antioxidant pattern could be attributed to the increased bacteria diversity, stimulated probiotics ( and ) and short-chain fatty acid (SCFA) producers (, , and ) improved by anthocyanins and their metabolites, which improved the colon environment, characterized by promoted SCFAs, restored colonic mucosa, and reorganized microbial structure. Thus, anthocyanin-rich dietary intervention is a promising approach for the defensiveness in human oxidative damage and neurodegeneration.
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http://dx.doi.org/10.1021/acs.jafc.0c07637DOI Listing
March 2021

The effect of pH on the chemical and structural interactions between apple polyphenol and starch derived from rice and maize.

Food Sci Nutr 2020 Sep 29;8(9):5026-5035. Epub 2020 Jul 29.

College of Food Science Shenyang Agricultural University Shenyang China.

To date, how pH affects starch-polyphenol mixtures has not been thoroughly investigated. This study explored the impact of combining apple polyphenol (AP) with both normal rice starch (NRS) and normal maize starch (NMS) across a range of pH conditions. NRS-AP mixture particle sizes across a pH range of 3-8 varied from 169.9 ± 5.4 to 187.5 ± 6.9 μm, while for NMS-AP particles, these sizes ranged from 161.8 ± 8.0 to 176.0 ± 4.9 μm, indicating that the aggregation of starch-AP was inhibited under low pH condition. The melting enthalpy (△H) values of the NRS-AP mixture across a pH range of 3-8 were 8.50 ± 0.06-9.56 ± 0.12 J/g, while the corresponding value for the NMS-AP mixture was 5.77 ± 0.05-6.21 ± 0.08 J/g. FTIR analyses revealed that the degree of order of these starch-AP mixtures significantly decreased under low pH conditions. XRD analysis further revealed that both NRS-AP and NMS-AP mixtures exhibited V-type structures, and relative crystallinity levels decreased significantly under low pH conditions. Together, these results indicate that low pH values inhibit the recrystallization of NRS-AP and NMS-AP mixtures. Overall, these findings provide additional evidence regarding the interactions between AP and specific starches under a range of pH conditions.
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http://dx.doi.org/10.1002/fsn3.1800DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7500801PMC
September 2020

Effects of α-casein and β-casein on the stability, antioxidant activity and bioaccessibility of blueberry anthocyanins with an in vitro simulated digestion.

Food Chem 2021 Jan 10;334:127526. Epub 2020 Jul 10.

College of Food Science, Shenyang Agricultural University, Shenyang, Liaoning 110866, China. Electronic address:

Blueberry anthocyanins are well-known for their diverse biological functions. However, the instability during digestion results in their weak bioavailability. The current study aimed to investigate the alteration in the stability, antioxidant capacity and bioaccessibility of blueberry anthocyanins with the addition of α-casein and β-casein in a simulated digestion system using pH differential method, HPLC-MS analysis, peroxyl scavenging capacity (PSC) assay, cellular antioxidant activity (CAA) and penetration test. The results showed that both α-casein and β-casein could increase the stability of blueberry anthocyanins during intestinal digestion and protect their antioxidant capacity. Moreover, the addition of α-casein or β-casein would enhance the bioaccessibility of blueberry anthocyanins. In conclusion, our study highlights that the interaction between α-casein or β-casein with blueberry anthocyanins can protect the compounds against influences associated with the simulated digestion.
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http://dx.doi.org/10.1016/j.foodchem.2020.127526DOI Listing
January 2021

Serum Ceramide Reduction by Blueberry Anthocyanin-Rich Extract Alleviates Insulin Resistance in Hyperlipidemia Mice.

J Agric Food Chem 2020 Aug 21;68(31):8185-8194. Epub 2020 Jul 21.

College of Food Science, Shenyang Agricultural University, Shenyang, Liaoning 110866, People's Republic of China.

Blueberry anthocyanin-rich extract (BAE) was supplemented to high-fat diet (HFD)-fed mice to investigate sphingolipid metabolism modulating factors involved in the attenuated hyperinsulinemia and hyperlipidemia. A BAE-containing diet effectively controlled food intake and liver weight and significantly attenuated insulin resistance triggered by a HFD. Higher BAE (200 mg/kg of body weight) administration performed more efficiently in the improvement of hepatic steatosis and adipocyte hypertrophy, together with distinct suppressions in serum triacylglycerol and cholesterol in total and species. Serum lipid compositions revealed 200 mg/kg of BAE supplementation remarkably suppressed ceramide accumulation. Consistently, genes encoding enzymes associated with sphingomyelin conversion and ceramide synthesis were modulated toward a healthy direction for restrained sphingolipid accumulation. Further, the inhibited mRNA expressions of protein phosphatase 2A and protein kinase Cζ involved in blocking Akt phosphorylation connected the controlled ceramides with the restored insulin sensitivity.
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http://dx.doi.org/10.1021/acs.jafc.0c01931DOI Listing
August 2020

Effect of ultrahigh pressure on structural and physicochemical properties of rice and corn starch in complexes with apple polyphenols.

J Sci Food Agric 2020 Dec 16;100(15):5395-5402. Epub 2020 Aug 16.

College of Food Science, Shenyang Agricultural University, Shenyang, PR China.

Background: Ultrahigh-pressure (UHP) treatment, a non-thermal processing technology, exerts a bactericidal effect and affects food texture. How UHP treatments influence starch-polyphenol complexes has not yet been reported. Here, we studied the effects of UHP treatment on the structure of common rice starch (CRS)-apple polyphenol (AP) and common corn starch (CCS)-AP mixtures.

Results: Overall, UHP treatment decreased the particle size of the CRS-AP and CCS-AP composites. Furthermore, the ΔH values of the CRS-AP and CCS-AP mixtures decreased, and the heating stability was improved after UHP treatment. X-ray diffraction indicated that the relative crystallinity of the mixtures was unaffected by UHP treatment. Fourier-transform infrared spectroscopy proved that no new absorption peaks were observed in the infrared spectra, and the order of starch-AP was decreased after UHP treatment. These results indicated that UHP treatment inhibited the retrogradation of the starch-AP mixture. Our analyses of the microstructures of CRS-AP and CCS-AP mixtures showed increased folding and more pronounced network structures under high-pressure.

Conclusions: These results provide a theoretical basis for further exploring the properties of starch-AP mixtures following UHP treatment and provide insights regarding the use of UHP treatments for food production. © 2020 Society of Chemical Industry.
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http://dx.doi.org/10.1002/jsfa.10589DOI Listing
December 2020

In vitro antioxidant capacities of eight different kinds of apples and their effects on lipopolysaccharide-induced oxidative damage in mice.

PLoS One 2018 25;13(1):e0191762. Epub 2018 Jan 25.

College of Food Science, Shenyang Agricultural University, Liaoning, China.

In the present study, the DPPH and ABTS+ radical scavenging activity of eight types of apples decreased (P < 0.05) during the 70-day storage at 4°C. The Fushi (F2) apples from Xin Jiang showed the highest radical scavenging activity. For in vivo study, 40 male Kunming mice (body weight 20-25 g) were selected and randomly assigned to four groups (10 mice per group). The F2 groups (F2S, F2 + sterile saline and F2L, F2 + lipopolysaccharide) were administered with 0.3 mL F2 filtrate via gastric intubation daily for 28 days. The control groups (CS, CON + sterile saline and CL, CON + lipopolysaccharide) were treated with sterile saline at the same volume. At day 29, mice of F2L and CL groups were injected with 100 μg/kg body weight of lipopolysaccharide (LPS) intraperitoneally, while those of F2S and CS groups were injected equal volume of sterile saline. In comparison to the CS group, the CL group showed a decrease (P < 0.05) in serum, liver, and hepatic mitochondrial antioxidant capacity, reduction (P < 0.05) in the expression of hepatic antioxidant-related genes, and an increase (P < 0.05) in serum alanine aminotransferase (ALT), aspartate aminotransferase (AST), malondialdehyde (MDA), protein carbonyl (PC), and reactive oxygen species (ROS). In comparison to the CL group, the F2L group showed lower (P < 0.05) levels of serum ALT, AST, and ROS, higher (P < 0.05) level of serum, liver, and hepatic mitochondrial antioxidant capacity, increased mitochondrial membrane potential (MMP), and enhanced (P < 0.05) expression of hepatic antioxidant-related genes. These results suggest that F2 may exert protective effect against LPS-induced oxidative damage by improving the antioxidant capacity.
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http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0191762PLOS
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5784986PMC
March 2018
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