Publications by authors named "Huijie Jiang"

63 Publications

F-FDG texture analysis predicts the pathological Fuhrman nuclear grade of clear cell renal cell carcinoma.

Abdom Radiol (NY) 2021 Aug 28. Epub 2021 Aug 28.

Department of Nuclear Medicine, The First Affiliated Hospital of Harbin Medical University, Harbin, China.

Purpose: This article analyzes the image heterogeneity of clear cell renal cell carcinoma (ccRCC) based on positron emission tomography (PET) and positron emission tomography-computed tomography (PET/CT) texture parameters, and provides a new objective quantitative parameter for predicting pathological Fuhrman nuclear grading before surgery.

Methods: A retrospective analysis was performed on preoperative PET/CT images of 49 patients whose surgical pathology was ccRCC, 27 of whom were low grade (Fuhrman I/II) and 22 of whom were high grade (Fuhrman III/IV). Radiological parameters and standard uptake value (SUV) indicators on PET and computed tomography (CT) images were extracted by using the LIFEx software package. The discriminative ability of each texture parameter was evaluated through receiver operating curve (ROC). Binary logistic regression analysis was used to screen the texture parameters with distinguishing and diagnostic capabilities and whose area under curve (AUC) > 0.5. DeLong's test was used to compare the AUCs of PET texture parameter model and PET/CT texture parameter model with traditional maximum standardized uptake value (SUVmax) model and the ratio of tumor SUVmax to liver SUVmean (SUL)model. In addition, the models with the larger AUCs among the SUV models and texture models were prospectively internally verified.

Results: In the ROC curve analysis, the AUCs of SUVmax model, SUL model, PET texture parameter model, and PET/CT texture parameter model were 0.803, 0.819, 0.873, and 0.926, respectively. The prediction ability of PET texture parameter model or PET/CT texture parameter model was significantly better than SUVmax model (P = 0.017, P = 0.02), but it was not better than SUL model (P = 0.269, P = 0.053). In the prospective validation cohort, both the SUL model and the PET/CT texture parameter model had good predictive ability, and the AUCs of them were 0.727 and 0.792, respectively.

Conclusion: PET and PET/CT texture parameter models can improve the prediction ability of ccRCC Fuhrman nuclear grade; SUL model may be the more accurate and easiest way to predict ccRCC Fuhrman nuclear grade.
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http://dx.doi.org/10.1007/s00261-021-03246-xDOI Listing
August 2021

Dysbiosis of Gut Microbiota in Patients With Acute Myocardial Infarction.

Front Microbiol 2021 5;12:680101. Epub 2021 Jul 5.

Department of Information Engineering, Heilongjiang Biological Science and Technology Career Academy, Harbin, China.

Acute myocardial infarction (AMI) continues as the main cause of morbidity and mortality worldwide. Interestingly, emerging evidence highlights the role of gut microbiota in regulating the pathogenesis of coronary heart disease, but few studies have systematically assessed the alterations and influence of gut microbiota in AMI patients. As one approach to address this deficiency, in this study the composition of fecal microflora was determined from Chinese AMI patients and links between gut microflora and clinical features and functional pathways of AMI were assessed. Fecal samples from 30 AMI patients and 30 healthy controls were collected to identify the gut microbiota composition and the alterations using bacterial 16S rRNA gene sequencing. We found that gut microflora in AMI patients contained a lower abundance of the phylum and a slightly higher abundance of the phylum compared to the healthy controls. Chao1 ( = 0.0472) and PD-whole-tree ( = 0.0426) indices were significantly lower in the AMI versus control group. The AMI group was characterized by higher levels of the genera , , , and , and lower levels of , , , , and as compared to that in the healthy controls ( < 0.05). The common metabolites of these genera are mostly short-chain fatty acids, which reveals that the gut flora is most likely to affect the occurrence and development of AMI through the short-chain fatty acid pathway. In addition, our results provide the first evidence revealing remarkable differences in fecal microflora among subgroups of AMI patients, including the STEMI vs. NSTEMI, IRA-LAD vs. IRA-Non-LAD and Multiple (≥2 coronary stenosis) vs. Single coronary stenosis groups. Several gut microflora were also correlated with clinically significant characteristics of AMI patients, including LVEDD, LVEF, serum TnI and NT-proBNP, Syntax score, counts of leukocytes, neutrophils and monocytes, and fasting serum glucose levels. Taken together, the data generated enables the prediction of several functional pathways as based on the fecal microfloral composition of AMI patients. Such information may enhance our comprehension of AMI pathogenesis.
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http://dx.doi.org/10.3389/fmicb.2021.680101DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8290895PMC
July 2021

Inhibition of the Nrf2/p38MAPK pathway involved in deltamethrin-induced apoptosis and fibrosis in quail kidney.

Food Chem Toxicol 2021 Sep 30;155:112382. Epub 2021 Jun 30.

College of Veterinary Medicine, Northeast Agricultural University, Harbin, 150030, China; Heilongjiang Key Laboratory for Laboratory Animals and Comparative Medicine, Harbin, 150030, China. Electronic address:

Deltamethrin (DLM) is a broad-spectrum and effective pyrethroid insecticide. However, DLM has good residual activity on most surfaces and many insects, so it poses a threat to the environment and health of animals and human. Exposure to DLM can cause kidney injury, but the mechanism is not well understood. Therefore, we investigated the possible mechanism of quail kidney injury induced by chronic exposure to different doses of DLM for 12 weeks. The results showed that chronic exposure to DLM induced apoptosis and fibrosis of quail kidney through the promotion of oxidative stress by down-regulating nuclear factor erythroid 2 related factor 2 (Nrf2), up-regulating the phosphorylation of p38 mitogen-activated protein kinases (p38MAPK). Furthermore, DLM-induced kidney apoptosis in quails as evidenced by increased expression of B-cell lymphoma gene 2-associated X while decreased expression of B-cell lymphoma-extra large. Simultaneously, DLM-induced kidney fibrosis in quails as evidenced by increased expression of fibrosis maker proteins. Overall, the results demonstrate that chronic DLM exposure induces kidney apoptosis and fibrosis via inhibition of the Nrf2/p38MAPK pathway. This study provides a new understanding for the mechanism of DLM-induced quail kidney injury and also provides a theoretical basis for treatment of the DLM poisoning.
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http://dx.doi.org/10.1016/j.fct.2021.112382DOI Listing
September 2021

A radiomics-based nomogram for preoperative T staging prediction of rectal cancer.

Abdom Radiol (NY) 2021 10 3;46(10):4525-4535. Epub 2021 Jun 3.

Department of Interventional Radiology, The Second Affiliated Hospital of Harbin Medical University, Harbin, China.

Purpose: To investigate the value of a radiomics-based nomogram in predicting preoperative T staging of rectal cancer.

Methods: A total of 268 eligible rectal cancer patients from August 2012 to December 2018 were enrolled and allocated into two datasets: training (n = 188) and validation datasets (n = 80). Another set of 32 patients from January 2019 to July 2019 was included in a prospective analysis. Pretreatment T2-weighted images were used to radiomics features extraction. Feature selection and radiomics score (Rad-score) construction were performed through a least absolute shrinkage and selection operator regression analysis. The nomogram, which included Rad-scores and clinical factors, was built using multivariate logistic regression. Discrimination, calibration, and clinical utility were used to evaluate the performance of the nomogram.

Results: The Rad-score containing nine selected features was significantly related to T staging. Patients who had locally advanced rectal cancer (LARC) generally had higher Rad-scores than patients with early-stage rectal cancer. The nomogram incorporated Rad-scores and carcinoembryonic antigen levels and showed good discrimination, with an area under the curve (AUC) of 0.882 (95% confidence interval [CI] 0.835-0.930) in the training dataset and 0.846 (95% CI 0.757-0.936) in the validation dataset. The calibration curves confirmed high goodness of fit, and the decision curve analysis revealed the clinical value. A prospective analysis demonstrated that the AUC of the nomogram to predict LARC was 0.859 (95% CI 0.730-0.987).

Conclusion: A radiomics-based nomogram is a novel method for predicting LARC and can provide support in clinical decision making.
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http://dx.doi.org/10.1007/s00261-021-03137-1DOI Listing
October 2021

Study on the prognosis predictive model of COVID-19 patients based on CT radiomics.

Sci Rep 2021 06 2;11(1):11591. Epub 2021 Jun 2.

Department of Radiology, Longhua Hospital,, Shanghai University of Traditional Chinese Medicine, No.725, South Wanping Road, Shanghai, 200032, China.

Making timely assessments of disease progression in patients with COVID-19 could help offer the best personalized treatment. The purpose of this study was to explore an effective model to predict the outcome of patients with COVID-19. We retrospectively included 188 patients (124 in the training set and 64 in the test set) diagnosed with COVID-19. Patients were divided into aggravation and improvement groups according to the disease progression. Three kinds of models were established, including the radiomics, clinical, and combined model. Receiver operating characteristic curves, decision curves, and Delong's test were used to evaluate and compare the models. Our analysis showed that all the established prediction models had good predictive performance in predicting the progress and outcome of COVID-19.
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http://dx.doi.org/10.1038/s41598-021-90991-0DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8172890PMC
June 2021

Pulmonary inflammatory and fibrogenic response induced by graphitized multi-walled carbon nanotube involved in cGAS-STING signaling pathway.

J Hazard Mater 2021 09 4;417:125984. Epub 2021 May 4.

College of Veterinary Medicine, Northeast Agricultural University, Harbin 150030, China; Heilongjiang Key Laboratory for Laboratory Animals and Comparative Medicine, Harbin 150030, China. Electronic address:

Graphitized multi-walled carbon nanotubes (GMWCNTs) are a new type of nanomaterial. Recently, their production and application in biological medicine have grown rapidly. However, GMWCNTs may cause adverse health effects, including the common occupational disease of pulmonary fibrosis. Pulmonary fibrosis is a serious progressive disease that often leads to lung failure, high mortality, and disability, and there is no effective therapy currently available. Therefore, identifying new biomarkers of the disease is important to better understand the disease mechanisms and explore new therapeutic strategies. In this study, 40 μg of GMWCNTs was used to treat mice in vivo by pharyngeal aspiration, and different genes were screened by transcriptome sequencing. Activation of the cyclic GMP-AMP synthase (cGAS)-stimulator of interferon gene (STING) signal pathway had an important effect on the development of pulmonary inflammation and fibrosis. GMWCNTs were then administered to the mice with a STING inhibitor (C-176). Inhibition of STING effectively decreased pulmonary inflammation and fibrosis in mice induced by GMWCNTs. Collectively, activation of the cGAS-STING signaling pathway is involved in GMWCNT-induced pulmonary inflammation and fibrosis in mice.
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http://dx.doi.org/10.1016/j.jhazmat.2021.125984DOI Listing
September 2021

Toxicological effects of deltamethrin on quail cerebrum: Weakened antioxidant defense and enhanced apoptosis.

Environ Pollut 2021 Oct 6;286:117319. Epub 2021 May 6.

College of Veterinary Medicine, Northeast Agricultural University, Harbin, 150030, China; Heilongjiang Key Laboratory for Laboratory Animals and Comparative Medicine, Harbin, 150030, China. Electronic address:

Deltamethrin is the most common type II synthetic pyrethroid insecticide, and has posed widespread residues to environment. However, whether deltamethrin has potential toxic effects on quail cerebrum remains greatly obscure. Accordingly, we investigated the impact of chronic exposure to deltamethrin on oxidative stress and apoptosis in quail cerebrum. Quails upon 12-week exposure of deltamethrin (0, 15, 30, or 45 mg/kg body weight intragastric administration) were used as a cerebrum injury model. The results showed that deltamethrin treatment led to cerebral injury dose-dependently through the weakened antioxidant defense by downregulating nuclear factor erythroid-2-related factor 2 (Nrf2) and its downstream proteins levels and mRNA expression. Furthermore, deltamethrin treatment induced apoptosis in cerebrum by decreasing B-cell lymphoma gene 2 (Bcl-2) level, as well as increasing Jun N-terminal kinase3, caspase-3, and Bcl-2-associated X protein levels. Simultaneously, toll-like receptor 4 (TLR4) downstream inflammation-related genes or proteins were significantly up-regulated by deltamethrin dose-dependently. Altogether, our study demonstrated that chronic exposure to deltamethrin induces inflammation and apoptosis in quail cerebrums by promoting oxidative stress linked to inhibition of the Nrf2/TLR4 signaling pathway. These results provide a novel knowledge on the chronic toxic effect of deltamethrin, and establish a theoretical foundation for the evaluation of pesticide-induced health risk.
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http://dx.doi.org/10.1016/j.envpol.2021.117319DOI Listing
October 2021

Application of Field-of-View Optimized and Constrained Undistorted Single Shot (FOCUS) with Intravoxel Incoherent Motion (IVIM) in 3T in Locally Advanced Rectal Cancer.

Dis Markers 2021 20;2021:5565902. Epub 2021 Mar 20.

Department of Pathology, The Second Affiliated Hospital of Harbin Medical University, Harbin, 150086 Heilongjiang, China.

Purpose: To evaluate the efficacy of field-of-view (FOV) optimized and constrained undistorted single shot (FOCUS) with IVIM in 3T MRI in the grading of patients with locally advanced rectal cancer.

Methods: From January 1st to December 31st, 2019, patients with locally advanced rectal cancer were retrieved. FOCUS DWI and FOCUS IVIM were obtained. Apparent diffusion coefficient (ADC) and IVIM parameters including mean true diffusion coefficient (), pseudodiffusion coefficient associated with blood flow (∗), and perfusion fraction () of the tumor parenchyma and normal rectal wall, as well as the normalized tumor parameters by corresponding normal intestinal wall parameters (ADC, , ∗, and ), were compared between the well/moderately differentiated and poorly differentiated groups by Student's -test. The relationship between the above parameters and the histologic grade was analyzed using Spearman's correlation test, with the ROC curve generated.

Results: Eighty-eight patients (aged 31 to 77 years old, mean = 56) were included for analysis. and were positively correlated with the tumor grade ( = 0.483, < 0.001 and = 0.610, < 0.001, respectively). All the normalized parameters (ADC, , ∗, and ) were positively correlated with the tumor grade ( = 0.267, = 0.007; = 0.564, = 0.001; = 0.414, = 0.005; and = 0.605, < 0.001, respectively). The best discriminative parameter was the value, and the area under the ROC curve was 0.927. With a cut-off value of 22.0%, had a sensitivity of 88.9% and a specificity of 100%.

Conclusion: FOCUS IVIM-derived parameters and normalized parameters are useful for predicting the histologic grade in rectal cancer patients.
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http://dx.doi.org/10.1155/2021/5565902DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8055408PMC
March 2021

Correlation between glucose metabolism parameters derived from FDG and tumor TNM stages and metastasis-associated proteins in colorectal carcinoma patients.

BMC Cancer 2021 Mar 9;21(1):258. Epub 2021 Mar 9.

Department of Radiology, Beijing Friendship Hospital, Capital Medical University, No. 95 Yongan Road, Xicheng District, Beijing, China.

Background: The aim of this study was to investigate the relationship between multiple metabolism parameters derived from FDG and tumor TNM stages as well as tumor metastasis-associated protein of GLUT-1 and MACC1 in colorectal carcinoma (CRC).

Methods: Thirty-eight patients (24 males and 14 females) with primary CRC confirmed by elective surgery pathological, who also accepted F-FDG PET/CT scans during 2017 to 2019 were included in this study. The tumor classification of T, N and M is explained by the 7th American Joint Committee on Cancer (AJCC). F-FDG parameters of SUVmax, SUVmean, TLG and MTV were measured by drawing a region of interest on the primary lesions. The expression of GLUT-1 and MACC1 was quantified by immunohistochemical, and the correlation between metabolism parameters and tumor biomarkers were analyzed.

Results: According to our analysis, the F-FDG parameters of SUVmean was significantly correlated with tumor M status (P = 0.000) of primary CRC. The primary tumor lesion with higher SUVmax, TLG and MTV values prone to a high-T status (P = 0.002, 0.002 and 0.001, respectively). The high expression of GLUT-1/MACC1 weas more frequently involved with T3-4 stage and was poorly differentiated in CRC patients. Multivariate analysis found that the expression of GLUT-1 protein was correlated with SUVmax and MTV (R = 0.42, P = 0.013 and 0.004, respectively), moreover, the expression of MACC1 protein was correlated with TLG (R = 0.372, P = 0.000).

Conclusion: Glucose metabolism parameters derived from FDG provides a noninvasive assessment of M status and T status in CRC patients. The expression of GLUT-1 and MACC1 was associated with F-FDG uptake in CRC patients.
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http://dx.doi.org/10.1186/s12885-021-07944-zDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7941722PMC
March 2021

Screening of radiotracer for diagnosis of colorectal cancer liver metastasis based on MACC1-SPON2.

Abdom Radiol (NY) 2021 07 13;46(7):3227-3237. Epub 2021 Mar 13.

Department of Radiology, Longhua Hospital Shanghai University of Traditional Chinese Medicine, Shanghai, China.

Background: Metastasis-associated in colon cancer 1 (MACC1) and Spondin2 (SPON2) are newly discovered oncogenes, but little is known about their role in colorectal cancer(CRC) liver metastases. PET has become an important molecular imaging technology due to its high sensitivity and quantifiability. In particular, its targeted, specific molecular probes can detect biological behaviors. This study was designed to evaluate the different biological properties of F-FDG, F-FLT, and F-FMISO PET. The value of the CRC liver metastasis model explores the correlation and potential mechanisms of three tracers uptakes with tumor-related biological characteristics.

Methods: Human CRC cell lines(LoVo and HCT8), were cultured for in vitro radionuclide uptake experiments to compare the molecular imaging features of colorectal cancer cells with different metastatic potentials. Two kinds of cells were injected into the spleen of nude mice to establish a liver metastasis model. After the tumor formation, three kinds of tracer PET images were performed to evaluate the characteristics of live PET imaging of high and low liver metastasis colorectal cancer models. The expression levels of MACC1 and SPON2 in tissues were detected by immunohistochemistry and Western blot. Correlation between tracer uptake and expression of MACC1 and SPON2 in liver metastases was assessed by linear regression analysis.

Results: The uptake rate of in vitro three tracers uptake experiments was LoVo > HCT8. Micro-PET scan showed no significant difference between the F-FDG SUV values of the two cells (P > 0.05); there was significant difference between the F-FLT and F-FMISO SUV values (P < 0.05). All in vivo FLT and FMISO SUV values were significantly higher in LoVo tumors than in HCT8 tumors. The results of Western blot and immunohistochemistry showed that the expression levels of MACC1 and SPON2 in LoVo liver metastasis were higher than those in HCT8 (P < 0.05). The F-FLT SUVmax ratio was significantly correlated with the expression of MACC1 and SPON2 in hepatic metastases (r = 0.737, P = 0.0026; r = 0.842, P = 0.0002). The F-FMISO SUVmax ratio was only significantly correlated with the expression of MACC1 in hepatic metastasis (r = 0.770, P = 0.0013).

Conclusions: Early screening with F-FLT and F-FMISO tracers has important clinical value for the efficient diagnosis and treatment of colorectal cancer liver metastases.
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http://dx.doi.org/10.1007/s00261-021-03015-wDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8215036PMC
July 2021

A comprehensive quality evaluation method of Corydalis yanhusuo by HPLC fingerprints, chemometrics, and correlation analysis.

J Sep Sci 2021 May 31;44(10):2054-2064. Epub 2021 Mar 31.

College of Pharmaceutical Science, Zhejiang University of Technology, Hangzhou, 310014, P. R. China.

A novel quality evaluation method of Corydalis yanhusuo was established by researching the high-performance liquid chromatography behavior of alkaloids under different buffer solutions and exploring the correlation between alkaloids in C. yanhusuo. The retention times of tetrahydropalmatine and corydaline were significantly influenced by pH, while the peak shape was affected by buffer types and ionic strength. The resolution of compounds in fingerprint was satisfactory under acetonitrile-0.2% phosphoric acid buffer (adjusted pH to 5.0 with triethylamine). Twelve common peaks were found by comparing 20 batches of C. yanhusuo fingerprints, and three tertiary alkaloids and four quaternary alkaloids were identified. The fingerprints were analyzed by similarity analysis, hierarchical cluster analysis, principal component analysis, and partial least squares discriminant analysis. All samples were divided into three groups, and the contents of dehydrocorydaline and coptisine from Zhejiang province were relatively higher than other origins. There were six components performing more contributions to the quality of C. yanhusuo. The correlations between alkaloids were conducted by Pearson correlation analysis and mathematical model analysis. The content correlation between palmatine and berberine was y = 0.28x  + 0.03x + 0.03, and the dehydrocorydaline and coptisine was y = -7.54/(1 + (x/0.14) ) + 2.61. The established mathematical model of alkaloids provided a guiding significance for the quality control of C. yanhusuo.
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http://dx.doi.org/10.1002/jssc.202001250DOI Listing
May 2021

Novel thermally activated delayed fluorescence nano-micelle for tumor imaging.

Photodiagnosis Photodyn Ther 2021 Mar 8;33:102178. Epub 2021 Jan 8.

Department of Radiology, Second Affiliated Hospital of Harbin Medical University, Harbin 150086, China. Electronic address:

A novel aromatic-imide-based, thermally activated delayed fluorescence material nano-micelle (TADF-NM) compound was prepared after being encapsulated with DSPE-mPEG amphiphilic copolymers. TADF-NM has preferential characteristics such as biocompatibility, non-toxic and targeted ability, and importantly TADF-NM efficiently isolated oxygen to enhance the fluorescence lifetime, and prevented quenching for using time-resolved fluorescence imaging (TRFI) in tumor cells. The fluorescence lifetime of TADF-NM was about 212 μs in PBS and 112 μs in tumor cells, which was the longest in fluorescence lifetime images based on TADF materials. These studies have shown that TADF-NM have excellent potential ability to overcome the interference of auto-fluorescence while being applied in confocal fluorescence tumor imaging.
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http://dx.doi.org/10.1016/j.pdpdt.2021.102178DOI Listing
March 2021

MDCC-Net: Multiscale double-channel convolution U-Net framework for colorectal tumor segmentation.

Comput Biol Med 2021 03 16;130:104183. Epub 2020 Dec 16.

Shenzhen Institutes of Advanced Technology, Chinese Academy of Sciences, Shenzhen, China. Electronic address:

Purpose: Multiscale feature fusion is a feasible method to improve tumor segmentation accuracy. However, current multiscale networks have two common problems: 1. Some networks only allow feature fusion between encoders and decoders of the same scale. It is obvious that such feature fusion is not sufficient. 2. Some networks have too many dense skip connections and too much nesting between the coding layer and the decoding layer, which causes some features to be lost and means that not enough information will be learned from multiple scales. To overcome these two problems, we propose a multiscale double-channel convolution U-Net (MDCC-Net) framework for colorectal tumor segmentation.

Methods: In the coding layer, we designed a dual-channel separation and convolution module and then added residual connections to perform multiscale feature fusion on the input image and the feature map after dual-channel separation and convolution. By fusing features at different scales in the same coding layer, the network can fully extract the detailed information of the original image and learn more tumor boundary information.

Results: The segmentation results show that our proposed method has a high accuracy, with a Dice similarity coefficient (DSC) of 83.57%, which is an improvement of 9.59%, 6.42%, and 1.57% compared with nnU-Net, U-Net, and U-Net++, respectively.

Conclusion: The experimental results show that our proposed method has good performance in the segmentation of colorectal tumors and is close to the expert level. The proposed method has potential clinical applicability.
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http://dx.doi.org/10.1016/j.compbiomed.2020.104183DOI Listing
March 2021

CT findings of severe novel coronavirus disease (COVID-19): A case report of Heilongjiang Province, China.

Open Med (Wars) 2020 13;15(1):563-566. Epub 2020 Jun 13.

Department of Radiology, The Second Affiliated Hospital of Harbin Medical University, Heilongjiang Province, China.

2019 novel coronavirus disease (COVID-19, previously known as novel coronavirus pneumonia) was first discovered in December 2019 and spread widely in China and all over the world in 2020. The initial symptoms of most patients include fever, cough, and fatigue. Dyspnea may occur with the progress of the disease, and acute respiratory distress syndrome may occur in severe cases. The CT manifestations of this disease are mainly ground-glass opacity (GGO) in the lung, which may be accompanied by patchy consolidation, and fibrous changes may appear in the lung at the later stage of the disease. Combined with typical clinical and imaging findings and positive nucleic acid test results, the disease can be diagnosed. We report the first case of novel coronavirus disease (COVID-19) in Heilongjiang Province, China. The patient was seriously ill, who felt that he suffered from fever, fatigue, cough, and expectoration and sought medical treatment, with a history of contact with Wuhan. The leukocyte count was normal, and the lymphocyte count was decreased. CT imaging showed large GGO and partial patchy consolidation in both lungs. The patient recovered and was discharged after 26 days of treatment. This study is helpful for early diagnosis and timely clinical management by mastering the typical imaging of novel coronavirus disease (COVID-19).
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http://dx.doi.org/10.1515/med-2020-0151DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7711862PMC
June 2020

Preparation of Poly[lactic-co-glycolic] Acid Nanospheres and Its Role in Hepatoma Cells.

J Nanosci Nanotechnol 2021 Feb;21(2):977-986

Department of Radiology, The Second Affiliated Hospital of Harbin Medical University, Harbin 150086, Heilongjiang Province, China.

Poly[lactic-co-glycolic] acid (PLGA) targeting nanoparticles AFP/PLGA/Dt, loaded with Dt plasmid of diphtheria toxin gene, modified by Alpha fetoprotein (AFP) monoclonal antibody, is prepared. Its physical and chemical properties and its effect on HepG2 cells are studied. Firstly, Dt expression plasmid pET11a/Dt is constructed and PLGA nanoparticles are prepared by emulsion solvent evaporation (ESE). Scanning electron microscope (SEM) is used to observe its morphology. Laser Particle Sizer is used to measure the particle size. In addition, the encapsulation efficiency, drug loading and release rate of PLGA nanoparticles are measured. Carboxy fluorescein and rhodamine fluorescein are used to double label IgG/PLGA/Dt and AFP/PLGA/Dt nanospheres, respectively, the entry of nanospheres into HepG2 cells are observed at 3 h and 12 h. The effect of AFP/PLGA/Dt nanospheres on the migration of HepG2 cells is examined by wounding healing assay. Transwell chamber experiment is used to detect the effect of AFP/PLGA/Dt nanospheres on the invasion of HepG2 cells. MTT method is utilized to determine the inhibitory activity of nanoparticles on HepG2 cell proliferation. After treated with IgG/PLGA/Dt and AFP/PLGA/Dt nanoparticles for 48 hours, flow cytometry is used to detect the apoptosis rate and cell cycle of HepG2 cells in each group. The results show that the prepared nanospheres have regular morphology, flat surface, average particle size of 265.72±12.46 nm, zeta potential of -18.15 mV. The average entrapment efficiency and drug loading are 78.48±1.71% and 3.16±0.35%, respectively. The nanoparticles release slowly and stably . At the 10th day, the release rate reaches 75.13%. PLGA nanospheres can effectively protect DNA from nuclease degradation. The results show that AFP/PLGA/Dt nanospheres have biological targeting effect and can be enriched in cells. AFP/PLGA/Dt nanoparticles can significantly inhibit the migration, invasion and proliferation of HepG2 cells, and promote apoptosis.
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http://dx.doi.org/10.1166/jnn.2021.18627DOI Listing
February 2021

Inflammation response after the cessation of chronic arsenic exposure and post-treatment of natural astaxanthin in liver: potential role of cytokine-mediated cell-cell interactions.

Food Funct 2020 Oct;11(10):9252-9262

College of Veterinary Medicine, Northeast Agricultural University, 600 Changjiang Road, Harbin 150030, China. and Heilongjiang Key Laboratory for Laboratory Animals and Comparative Medicine, 600 Changjiang Road, Harbin 150030, China.

Ongoing groundwater arsenic contamination throughout China was first recognized in the 1960s. Groundwater arsenic contamination is a high risk for human and animal health worldwide. Apart from drinking water, diet is the second pathway for arsenic to enter the human body and eventually cause liver injury. Natural astaxanthin extracted from the green algae Haematococcus pluvialis has dominated the nutraceutical market for potential health benefits. Nevertheless, the molecular mechanism underlying the protective effect post astaxanthin against arsenic-induced hepatotoxicity remains largely obscure. In this study, we investigate the effect of natural astaxanthin (derived from Haemotococcus pluvialis) on oxidative stress and liver inflammatory response in rats after the cessation of chronic arsenic exposure. Wistar rats were given astaxanthin (250 mg kg-1) daily for 2 weeks after the cessation of exposure to sodium arsenite (300 μg L-1, drinking water, 24 weeks) by intragastric administration. The results showed that post treatment with astaxanthin attenuated liver injury induced by long-term exposure to arsenic in rats. Most importantly, post treatment with astaxanthin decreased the increasing of inflammatory cytokine NF-κB, tumor necrosis factor-α, interleukin-1β, oxidative stress level, and total arsenic content in livers of rats exposed to arsenic. In addition, post treatment with astaxanthin reversed the increasing of protein levels of alpha-smooth muscle actin and collagen Iα1, which are the activation markers of hepatic stellate cells (HSCs). Collectively, these data demonstrate that post astaxanthin treatment attenuates inflammation response in the liver after the cessation of chronic arsenic exposure via inhibition of cytokine-mediated cell-cell interactions. Daily ingestion of natural astaxanthin might be a potential and beneficial candidate for the treatment of liver damage after the cessation of chronic exposure to sodium arsenite.
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http://dx.doi.org/10.1039/d0fo01223hDOI Listing
October 2020

Clinical imaging characteristics of inpatients with coronavirus disease-2019 in Heilongjiang Province, China: a retrospective study.

Aging (Albany NY) 2020 07 20;12(14):13860-13868. Epub 2020 Jul 20.

Department of Radiology, Harbin Hong'an Hospital, Harbin, China.

Objective: To investigate the clinical, laboratory, and radiological characteristics of patients with coronavirus disease-2019 (COVID-19) in Heilongjiang Province.

Results: Patients in the ICU group were older and their incidence of cardiovascular disease was higher than those in the non-ICU group. Lymphocyte levels were lower and neutrophil and D-dimer levels were higher in the ICU than that in the non-ICU group. Compared to the non-ICU group, the incidence of pulmonary consolidation and ground-glass opacity with consolidation was significantly higher in the ICU group, all lung lobes were more likely to be involved, with higher number of lung lobes and areas surrounding the bronchi. Of the 59 patients with COVID-19 in this group, 15 received mechanical ventilation. All intubated patients involved lung lobes, and a large number of lesions were observed in the area around the bronchial vessels.

Conclusion: Significant differences were observed in clinical symptoms, laboratory tests, and computed tomography features between the ICU and non-ICU groups.

Methods: A total of 59 patients with COVID-19, comprising 44 patients in the intensive care unit (ICU) and 15 in the non-ICU, were retrospectively analyzed. Characteristics of the two groups of patients were compared.
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http://dx.doi.org/10.18632/aging.103633DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7425485PMC
July 2020

Cerenkov luminescence imaging is an effective preclinical tool for assessing colorectal cancer PD-L1 levels in vivo.

EJNMMI Res 2020 Jun 15;10(1):64. Epub 2020 Jun 15.

Department of Radiology, The Second Affiliated Hospital of Harbin Medical University, Harbin, China.

Background: Preclinical and clinical studies have demonstrated that immunotherapy has effectively delayed tumor progression, and the clinical outcomes of anti-PD-1/PD-L1 therapy were related to PD-L1 expression level in the tumors. A I-labeled anti-PD-L1 monoclonal antibody tracer, I-PD-L1-Mab, was developed to study the target ability of noninvasive Cerenkov luminescence imaging in colorectal cancer xenograft mice.

Method: Anti-PD-L1 monoclonal antibody labeled with I (I-PD-L1-Mab), and in vitro binding assays were used to evaluate the affinity of I-PD-L1-Mab to PD-L1 and their binding level to different colorectal cancer cells, and compared with flow cytometry, Western blot analysis, and immunofluorescence staining. The clinical application value of I-PD-L1-Mab was evaluated through biodistribution and Cerenkov luminescence imaging, and different tumor-bearing models expressing PD-L1 were evaluated.

Results: I-PD-L1-Mab showed high affinity to PD-L1, and the equilibrium dissociation constant was 1.069 × 10 M. The competitive inhibition assay further confirmed the specific binding ability of I-PD-L1-Mab. In four different tumor-bearing models with different PD-L1 expression, the biodistribution and Cerenkov luminescence imaging showed that the RKO tumors demonstrated the highest uptake of the tracer I-PD-L1-Mab, with a maximum uptake of 1.613 ± 0.738% IA/g at 48 h.

Conclusions: There is a great potential for I-PD-L1-Mab noninvasive Cerenkov luminescence imaging to assess the status of tumor PD-L1 expression and select patients for anti-PD-L1 targeted therapy.
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http://dx.doi.org/10.1186/s13550-020-00654-wDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7295871PMC
June 2020

3.0 T MRI IVIM-DWI for predicting the efficacy of neoadjuvant chemoradiation for locally advanced rectal cancer.

Abdom Radiol (NY) 2021 01;46(1):134-143

Department of Radiology, The Second Affiliated Hospital of Harbin Medical University, Harbin, 150086, China.

Purpose: The purpose of this study was to determine the diagnostic performance of intravoxel incoherent motion (IVIM) on assessing response to neoadjuvant chemoradiation (nCRT) in patients with Locally Advanced Rectal Cancer (LARC).

Methods: 50 patients with rectal cancer who underwent magnetic resonance (MR) imaging before and after nCRT, the values of pre-nCRT and post-nCRT IVIM-DWI parameters apparent diffusion coefficient (ADC), diffusion coefficient (D), false diffusion coefficient (D*), and perfusion fraction (f), together with the percentage changes (∆% parametric value) induced by nCRT were calculated. According to the patient's response to nCRT, the patients were divided into pathological complete response (pCR) and non-pCR groups, Good Response (GR) group and Poor Response (PR) group, and the above values were compared between different groups. Univariate and multiple logistic regression analysis were done to investigate the relation between different parameters and patient nCRT. Draw ROC curve according to sensitivity and specificity, and compare its diagnostic efficacy.

Results: There were no significant differences in the baseline data of 50 patients. After nCRT, the ADC and D values for LARC increased significantly (all p < 0.05). The pCR group (n = 9) had higher preD*, pref, postD*, ∆%ADC and ∆%D values than the non-pCR group (n = 41) (all p < 0.05). The GR group (n = 17) exhibited higher post D, ∆%ADC and ∆%D values than the PR group (n = 33) (all p < 0.05). From the results of Logistic regression analysis found that ∆%ADC and ∆%D were significantly correlated with patients' response to nCRT. Based on ROC analysis, ∆%D had a higher area under the curve value than ∆%ADC (p = 0.009) in discriminating the pCR from non-pCR groups.

Conclusions: IVIM-DWI technology may be helpful in identifying the pCR and GR patients to nCRT for LARC.
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http://dx.doi.org/10.1007/s00261-020-02594-4DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7864832PMC
January 2021

miR-615-3p promotes the epithelial-mesenchymal transition and metastasis of breast cancer by targeting PICK1/TGFBRI axis.

J Exp Clin Cancer Res 2020 Apr 26;39(1):71. Epub 2020 Apr 26.

Department of Oncology, The Second Affiliated Hospital of Harbin Medical University, 246 Xuefu Road, Harbin, 150086, China.

Background: Increasing evidence indicates that epithelial-mesenchymal transition (EMT) can be regulated by microRNAs (miRNAs). miR-615-3p was shown to be involved in tumor development. However, the role of miR-615-3p in the metastasis of breast cancer remains largely unknown.

Methods: The expression of miR-615-3p in breast cancer cells and tissues was assessed by qRT-PCR and situ hybridization assays. Effects of miR-615-3p on tumor metastasis were evaluated with experiments in vitro and mouse model. EMT markers were detected by western blot and immunofluorescence assays. Molecular mechanism of miR-615-3p in the regulation of breast cancer cell metastasis was analyzed by Western Blot, Co-immunoprecipitation, and Luciferase assay.

Results: In the present study, we found that miR-615-3p was significantly elevated in breast cancer cells and tissues, especially in those with metastasis. In breast cancer cell lines, stable overexpression of miR-615-3p was sufficient to promote cell motility in vitro, and pulmonary metastasis in vivo, accompanied by the reduced expression of epithelial markers and the increased levels of mesenchymal markers. Further studies revealed that the reintroduction of miR-615-3p increased the downstream signaling of TGF-β, the type I receptor (TGFBRI) by targeting the 3'-untranslated regions (3'-UTR) of PICK1. PICK1 inhibits the binding of DICER1 to Smad2/3 and the processing of pre-miR-615-3p to mature miR-615-3p in breast cancer cells, thus exerting a negative feedback loop.

Conclusions: Our data highlight an important role of miR-615-3p in the molecular etiology of breast cancer, and implicate the potential application of miR-615-3p in cancer therapy.
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http://dx.doi.org/10.1186/s13046-020-01571-5DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7183699PMC
April 2020

Organic "receptor" fully covered few-layer organic-metal chalcogenides for high-performance chemiresistive gas sensing at room temperature.

Chem Commun (Camb) 2020 May;56(40):5366-5369

State Key Laboratory of Structural Chemistry, Fujian Institute of Research on the Structure of Matter, Chinese Academy of Sciences (CAS), No. 155 Yangqiao Road West, Fuzhou, Fujian 350002, P. R. China. and University of Chinese Academy of Sciences (UCAS), No. 19A Yuquan Road, Beijing 100049, P. R. China.

Organic-metal chalcogenides (OMCs) are proposed as a new family of two-dimensional (2D) chemiresistive sensing materials. Few-layer Ag(SPh-NH2), one of the OMCs, fully and orderly covered with predesigned -NH2 groups as "receptors", shows the highest sensitivity, excellent selectivity and reversibility in response to NO2 among all the reported 2D chemiresistive sensing materials at room temperature.
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http://dx.doi.org/10.1039/d0cc01092hDOI Listing
May 2020

Retrospective analysis of the prognostic value of PD-L1 expression and F-FDG PET/CT metabolic parameters in colorectal cancer.

J Cancer 2020 25;11(10):2864-2873. Epub 2020 Feb 25.

Department of PET/CT, The Second Affiliated Hospital of Harbin Medical University, Harbin, China.

It has been rarely reported whether F-fluorodeoxyglucose (F-FDG) uptake in colorectal cancer cells is associated with the expression of PD-L1. We performed a clinical pathology study to evaluate PD-L1 expression in patients undergoing surgical resection of colorectal cancer with preoperative F-FDG PET/CT imaging, with the aim of predicting the response of CRC patients to immune checkpoint inhibitors. : A retrospective analysis of patients with CRC who underwent FDG-PET imaging before surgery was performed to measure the parameters of FDG-PET imaging: the maximum standardized uptake value (SUVmax), the metabolic tumor volume (MTV), and the total lesion glycolysis (TLG) were evaluated to determine whether each parameter was associated with clinical pathology. Tumor specimens were subjected to PD-L1 staining by immunohistochemistry. Analysis of whether there is a correlation between PD-L1 expression and F-FDG uptake parameters in CRC. : PD-L1 expression level was significantly correlated with SUVmax, MTV3.0 and TLG3.0. Multivariate analysis showed that PD-L1 and TLG3.0 were independent predictors of poor DFS in patients with CRC (0.009; 0.016), PD-L1 expression is closely related to the patient's lesion (TLG3.0) (<0.01). : The results of this study indicate that there was a significant correlation between PD-L1 expression and TLG3.0 which suggested that FDG-PET could serve as a noninvasive tool to assess the tumor microenvironment and as a predictor of PD-L1 inhibitor activity to determine the optimal therapeutic strategy for CRC. High PD-L1 expression levels and high TLG3.0 are independent risk factors for DFS differences in CRC patients.
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http://dx.doi.org/10.7150/jca.38689DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7086272PMC
February 2020

LDL-C plays a causal role on T2DM: a Mendelian randomization analysis.

Aging (Albany NY) 2020 02 10;12(3):2584-2594. Epub 2020 Feb 10.

Cardiovascular Department, The Fourth Affiliated Hospital of Harbin Medical University, Harbin, China.

Diabetic dyslipidemia is a common condition in patients with Type 2 diabetes mellitus (T2DM). However, with the increasing application of statins which mainly decrease low-density lipoprotein cholesterol (LDL-C) levels, clinical trials and meta-analysis showed a clearly increase of the incidence of new-onset DMs, partly due to genetic factors. To determine whether a causal relationship exists between LDL-C and T2DM, we conducted a two-sample Mendelian Randomization (MR) analysis using genetic variations as instrumental variables (IVs). Initially, 29 SNPs significantly related to LDL-C (P≤ 5.0×10) were selected as based on results from the study of Henry et al, which processed loci data influencing lipids identified by the Global Lipids Genetics Consortium (GLGC) from 188,577 individuals of European ancestry. While 6 SNPs related to T2DM (P value < 5×10) were deleted, with the remaining 23 SNPs without LD eventually being deemed as IVs. The combined effect of all these 23 SNPs on T2DM, as generated with use of the penalized robust inverse-variance weighted (IVW) method (Beta value 0.24, 95%CI 0.087~0.393, P-value=0.002) demonstrated that elevated LDL-C levels significantly increased the risk of T2DM. The relationship between LDL-C and Type 1 diabetes mellitus (T1DM) with this analysis producing negative pooled results (Beta value -0.202, 95%CI -2.888~2.484, P-value=0.883).
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http://dx.doi.org/10.18632/aging.102763DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7041740PMC
February 2020

Integrating Multi-Omics Data to Identify Novel Disease Genes and Single-Neucleotide Polymorphisms.

Front Genet 2019 24;10:1336. Epub 2020 Jan 24.

Department of Information Engineering, Heilongjiang Biological Science and Technology Career Academy, Harbin, China.

Stroke ranks the second leading cause of death among people over the age of 60 in the world. Stroke is widely regarded as a complex disease that is affected by genetic and environmental factors. Evidence from twin and family studies suggests that genetic factors may play an important role in its pathogenesis. Therefore, research on the genetic association of susceptibility genes can help understand the mechanism of stroke. Genome-wide association study (GWAS) has found a large number of stroke-related loci, but their mechanism is unknown. In order to explore the function of single-nucleotide polymorphisms (SNPs) at the molecular level, in this paper, we integrated 8 GWAS datasets with brain expression quantitative trait loci (eQTL) dataset to identify SNPs and genes which are related to four types of stroke (ischemic stroke, large artery stroke, cardioembolic stroke, small vessel stroke). Thirty-eight SNPs which can affect 14 genes expression are found to be associated with stroke. Among these 14 genes, 10 genes expression are associated with ischemic stroke, one gene for large artery stroke, six genes for cardioembolic stroke and eight genes for small vessel stroke. To explore the effects of environmental factors on stroke, we identified methylation susceptibility loci associated with stroke using methylation quantitative trait loci (MQTL). Thirty-one of these 38 SNPs are at greater risk of methylation and can significantly change gene expression level. Overall, the genetic pathogenesis of stroke is explored from locus to gene, gene to gene expression and gene expression to phenotype.
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http://dx.doi.org/10.3389/fgene.2019.01336DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6993083PMC
January 2020

Sulforaphane prevents chromium-induced lung injury in rats via activation of the Akt/GSK-3β/Fyn pathway.

Environ Pollut 2020 Apr 18;259:113812. Epub 2019 Dec 18.

College of Veterinary Medicine, Northeast Agricultural University, Harbin, 150030, China; Heilongjiang Key Laboratory for Laboratory Animals and Comparative Medicine, Harbin, 150030, China. Electronic address:

Chromium (Cr) is an internationally recognized carcinogenic hazard that causes serious pulmonary toxicity. However, Cr-induced pulmonary toxicity lacks effective treatment to date. Sulforaphane (SFN), a well-known organosulfur compound, has gained increasing attention because of its unique biological function. This study investigates if SFN could decrease KCrO-induced pulmonary toxicity and a potential mechanism involved using a rat 35-day Cr-induced pulmonary toxicity model and the mouse alveolar type II epithelial cell line (MLE-12). The results showed that SFN prevented Cr-induced oxidative stress, histopathological lesions, inflammation, apoptosis, and changes in protein kinase B (Akt) and glycogen synthase kinase 3 beta (GSK-3β) levels in vivo and in vitro. However, SFN can not play the protective effect against KCrO-induced cell injury after treating by an Akt-specific inhibitor (MK-2206 2HCl) in MLE-12 cells. Furthermore, SFN increased the expression of nuclear factor-E2-related factor-2 (Nrf2) phase II detoxification enzymes. Collectively, this study demonstrates that SFN prevents KCrO-induced lung toxicity in rats through enhancing Nrf2-mediated exogenous antioxidant defenses via activation of the Akt/GSK-3β/Fyn signaling pathway. SFN may be a novel natural substance to cure Cr-induced lung toxicity.
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http://dx.doi.org/10.1016/j.envpol.2019.113812DOI Listing
April 2020

Transcriptome sequencing reveals phagocytosis as the main immune response in the pathogen-challenged sea urchin Strongylocentrotus intermedius.

Fish Shellfish Immunol 2019 Nov 1;94:780-791. Epub 2019 Oct 1.

Key Laboratory of Mariculture & Stock Enhancement in North China's Sea, Ministry of Agriculture and Rural Affairs, Dalian Ocean University, Dalian, 116023, PR China. Electronic address:

The clarification of host immune responses to causative bacteria of spotting disease in the sea urchin Strongylocentrotus intermedius is vital to preventing and controlling this disease, especially to selective breeding for disease resistance. For this purpose, sea urchins were challenged with the causative bacterium Vibrio sp. to obtain spotting diseased and undiseased samples. We conducted next-generation sequencing to assess the key genes/pathways in control (CG), diseased (DG), and undiseased (UG) groups. A total of 454.1 million clean reads were obtained and assembled into 23,899 UniGenes with an N50 of 1359 bp, with 86.11% of them matching the genome sequence of the sea urchin S. purpuratus. A total of 8415 UniGenes were mapped to the non-redundant database. Salmon expression analysis revealed 725 significantly differentially expressed genes (DEGs) among CG, DG, and UG. These DEGs were enriched into 72 Kyoto Encyclopedia of Genes and Genomes (KEGG) pathways, including a core set of immune correlated pathways notably in the phagosome, vitamin digestion and absorption, Wnt signaling, and Notch signaling pathways. DG was evidently upregulated in these immune pathways and could enhance phagocytosis directly or indirectly. Thus, phagocytosis was the main coelomic cellular immune response in S. intermedius challenged by spotting disease causative bacterium. The expression patterns of 10 DEGs were confirmed via RT-qPCR, and the expression levels were consistent with the results of RNA-seq. Furthermore, 9899 SSRs were identified, and 123,692, 151,827, and 114,368 candidate SNPs were identified from CG, DG, and UG, respectively. These results provide basic information for our understanding of sea urchin antibacterial immunity.
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http://dx.doi.org/10.1016/j.fsi.2019.10.002DOI Listing
November 2019

Evaluation of [F]FDG/[F]FLT/[F]FMISO-based micro-positron emission tomography in detection of liver metastasis in human colorectal cancer.

Nucl Med Biol 2019 May - Jun;72-73:36-44. Epub 2019 Jul 11.

Department of Radiology, Longhua Hospital Shanghai University of Traditional Chinese Medicine, Shanghai, China. Electronic address:

Introduction: Positron emission tomography (PET) is extensively used in clinical oncology for tumor detection. This study aimed to explore the application of the radiotracers [F]fluorodeoxyglucose ([F]FDG), 3'-deoxy-3'- [F]fluorothymidine ([F]FLT), and [F]fluoromisonidazole ([F]FMISO) in the diagnosis and monitoring of hepatic metastasis in human colorectal cancer (CRC).

Methods: A mouse model of human CRC with hepatic metastasis was established by intrasplenic implantation of human CRC cell lines LoVo or HCT8. Metastatic potential of these two cell lines was evaluated by wound healing assay in vitro and survival analysis. Uptake of radiotracers between LoVo and HCT8 cells and uptake of radiotracers in the resulting mouse tumor models were examined by in vivo and in vitro experiments. Uptake of each radiotracer in hepatic metastatic lesions was quantified and expressed as standard uptake value (SUV). Protein expression of multiple tumor biomarkers was determined in metastatic lesions. The correlation between tracer uptake and tumor marker expression was evaluated using linear regression.

Results: LoVo cells exhibited a stronger metastatic potential and a higher radiotracer uptake ability than HCT8 cells, as evidenced by significantly greater wound closure percentage, shorter survival, higher incidence of liver metastases, and higher cellular radiotracer levels in LoVo cells or LoVo cell-xenografted mice. SUV values of [F]FLT and [F]FMISO, but not [F]FDG, in LoVo cell-derived metastatic lesions were significantly greater than those in HCT8 lesions. Mechanistically, the expression of MACC1, HIF-1α, and GLUT-1(metastasis associated in colon cancer 1, MACC1; hypoxia-inducible factor 1-alpha, HIF-1α; and glucose transporter 1, GLUT-1, respectively) in LoVo cell-derived metastatic lesions was more effectively induced than in HCT8-derived ones. A linear regression analysis demonstrated significant positive correlations between [F]FLT/[F]FMISO uptake and tumor biomarker expression in metastatic tissues.

Conclusions: [F]FLT and [F]FMISO-based PET imaging may serve as a promising method for early detection and monitoring of hepatic metastasis in patients with CRC.
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http://dx.doi.org/10.1016/j.nucmedbio.2019.07.004DOI Listing
May 2020

Corrigendum: Exposing the Causal Effect of C-Reactive Protein on the Risk of Type 2 Diabetes Mellitus: A Mendelian Randomization Study.

Front Genet 2019 18;10:85. Epub 2019 Feb 18.

Department of Rehabilitation and Pharmacy, Heilongjiang Province Land Reclamation Headquarters General Hospital, Harbin, China.

[This corrects the article DOI: 10.3389/fgene.2018.00657.].
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http://dx.doi.org/10.3389/fgene.2019.00085DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6387998PMC
February 2019

Establishing the chromatographic fingerprint of traditional Chinese medicine standard decoction based on quality by design approach: A case study of Licorice.

J Sep Sci 2019 Mar 28;42(6):1144-1154. Epub 2019 Jan 28.

College of Pharmaceutical Science, Zhejiang University of Technology, Hangzhou, P. R. China.

A novel analytical quality by design approach for developing a chromatographic fingerprint was established for analyzing complex traditional Chinese medicine, using a licorice standard decoction as an example. Considering the characteristics of integrity and ambiguity, the resolution of eight common peaks, total peak number, capacity factor distributions, and peak purity were selected as potential critical method attributes for assessing the quality of the chromatographic fingerprint. A central composite design was used to evaluate the relationship between critical method attributes and critical method parameters, including column temperature, wavelength, flow rate, formic-acid concentration, and gradient parameters. A standard probability method was employed to calculate the design space of the fingerprint analysis parameters and evaluate the robustness of the methodology. The optimized high-performance liquid chromatography fingerprint conditions were acetonitrile and 0.1% formic acid water gradient elution (0-5 min, 5-19% A; 5-10 min, 19% A; 10-50 min, 19-42% A; 50-54 min, 42-100% A; 54-60 min, 100% A), column temperature 25±5°C, detection wavelength 265 nm. The design space of fingerprint analytical method based on the analytical quality by design approach not only met the requirements of the fingerprint analysis, but also improved the robustness and applicability of the fingerprint method.
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http://dx.doi.org/10.1002/jssc.201800989DOI Listing
March 2019

Exposing the Causal Effect of C-Reactive Protein on the Risk of Type 2 Diabetes Mellitus: A Mendelian Randomization Study.

Front Genet 2018 20;9:657. Epub 2018 Dec 20.

Heilongjiang Provincial Hospital, Harbin, China.

As a biomarker of inflammation, C-reactive protein (CRP) has attracted much attention due to its role in the incidence of type 2 diabetes mellitus (T2DM). Prospective studies have observed a positive correlation between the level of serum CRP and the incidence of T2DM. Recently, studies have reported that drugs for curing T2DM can also decrease the level of serum CRP. However, it is not yet clear whether high CRP levels cause T2DM. To evaluate this, we conducted a Mendelian randomization (MR) analysis using genetic variations as instrumental variables (IVs). Significantly associated single nucleotide polymorphisms (SNPs) of CRP were obtained from a genome-wide study and a replication study. Therein, 17,967 participants were utilized for the genome-wide association study (GWAS), and another 14,747 participants were utilized for the replication of identifying SNPs associated with CRP levels. The associations between SNPs and T2DM were from the DIAbetes Genetics Replication And Meta-analysis (DIAGRAM) consortium. After removing SNPs in linkage disequilibrium (LD) and T2DM-related SNPs, the four remaining CRP-related SNPs were deemed as IVs. To evaluate the pooled influence of these IVs on the risk of developing T2DM through CRP, the penalized robust inverse-variance weighted (IVW) method was carried out. The combined result (OR 1.114048; 95% CI 1.058656 to 1.172338; = 0.024) showed that high levels of CRP significantly increase the risk of T2DM. In the subsequent analysis of the relationship between CRP and type 1 diabetes mellitus (T1DM), the pooled result (OR 1.017145; 95% CI 0.9066489 to 1.14225; = 0.909) supported that CRP levels cannot determine the risk of developing T1DM.
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http://dx.doi.org/10.3389/fgene.2018.00657DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6306438PMC
December 2018
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