Publications by authors named "Huihui Li"

310 Publications

Tissue- and stage-specific landscape of the mouse translatome.

Nucleic Acids Res 2021 Jun 9. Epub 2021 Jun 9.

State Key Laboratory of Ophthalmology, Zhongshan Ophthalmic Center, Sun Yat-sen University, Guangzhou 510060, China.

The current understanding of how overall principles of translational control govern the embryo-to-adult transition in mammals is still far from comprehensive. Herein we profiled the translatomes and transcriptomes of six tissues from the mice at embryonic and adult stages and presented the first report of tissue- and stage-specific translational landscape in mice. We quantified the extent of gene expression divergence among different expression layers, tissues and stages, detected significant changes in gene composition and function underlying these divergences and revealed the changing architecture of translational regulation. We further showed that dynamic translational regulation can be largely achieved via modulation of translational efficiency. Translational efficiency could be altered by alternative splicing (AS), upstream and downstream open reading frames (uORFs and dORFs). We revealed AS-mediated translational repression that was exerted in an event type-dependent manner. uORFs and dORFs exhibited mutually exclusive usage and the opposing effects of translational regulation. Furthermore, we discovered many novel microproteins encoded by long noncoding RNAs and demonstrated their regulatory potential and functional relevance. Our data and analyses will facilitate a better understanding of the complexity of translation and translational regulation across tissue and stage spectra and provide an important resource to the translatome research community.
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http://dx.doi.org/10.1093/nar/gkab482DOI Listing
June 2021

Photodynamic inactivation of planktonic Staphylococcus aureus by sodium magnesium chlorophyllin and its effect on the storage quality of lettuce.

Photochem Photobiol Sci 2021 May 28. Epub 2021 May 28.

College of Food Science and Technology, Shanghai Ocean University, Shanghai, 201306, China.

Photodynamic inactivation (PDI) is a fast and effective non-heat sterilization technology. This study established an efficient blue light-emitting diode (LED) PDI with the photosensitizer sodium magnesium chlorophyllin (SMC) to eradicate Staphylococcus aureus in food. The antibacterial mechanisms were determined by evaluating DNA integrity, protein changes, morphological alteration, and the potency of PDI to eradicate S. aureus on lettuce was evaluated. Results showed that planktonic S. aureus could not be clearly observed on the medium after treatment with 5.0 μmol/L SMC for 10 min (1.14 J/cm). Bacterial cell DNA and protein were susceptible to SMC-mediated PDI, and cell membranes were found to be disrupted. Moreover, SMC-mediated PDI effectively reduced 8.31 log CFU/mL of S. aureus on lettuce under 6.84 J/cm radiant exposure (30 min) with 100 μmol/L SMC, and PDI displayed a potent ability to restrain the weight loss as well as retard the changes of color difference of the lettuce during 7 day storage. The study will enrich our understanding of the inactivation of S. aureus by PDI, allowing for the development of improved strategies to eliminate bacteria in the food industry.
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http://dx.doi.org/10.1007/s43630-021-00057-3DOI Listing
May 2021

Pyrotinib Combined With Vinorelbine in HER2-Positive Metastatic Breast Cancer: A Multicenter Retrospective Study.

Front Oncol 2021 20;11:664429. Epub 2021 Apr 20.

Huadong Hospital Affiliated to Fudan University, Shanghai, China.

Introduction: Pyrotinib plus capecitabine has been approved in China for human epidermal growth factor receptor 2 (HER2)-positive metastatic breast cancer (MBC). Meanwhile, vinorelbine is another important chemotherapy option for MBC available in oral and intravenous forms. Thus, pyrotinib plus vinorelbine may represent a new treatment option, particularly for patients with failed capecitabine treatment. This study reported the first real-world data for pyrotinib plus vinorelbine therapy in HER2+ MBC.

Methods: HER2+ MBC patients (n = 97) treated with pyrotinib plus vinorelbine in six institutions across China from May 2018 to June 2020 were enrolled. Progression-free survival (PFS), objective response rate (ORR), overall survival (OS), and toxicity profiles were determined.

Results: Sixty-seven percent of patients received more than two lines of systematic therapy. Nearly all patients (97.9%) had received trastuzumab and 50.5% were administered lapatinib. When combined with pyrotinib, 74.2% received oral and 25.8% received intravenous vinorelbine. Median PFS (mPFS) was 7.8 (range, 4.7-10.8) months for all patients. The mPFS in patients administered pyrotinib as second-line therapy and third-or-higher-line therapy were 12.0 and 6.4 months, respectively. Patients who received pyrotinib plus oral or intravenous vinorelbine had similar mPFS (7.8 vs. 6.4 months, p = 0.871). The 23 patients with brain metastases had mPFS of 6.3 (range, 3.4-9.2) months. Lapatinib-naïve patients had significantly longer PFS than lapatinib-treated patients (10.8 months vs. 5.6 months, p = 0.020). Median OS was not achieved. The ORR for 96 patients was 34.3%. Common grade 3 and 4 adverse events were diarrhea (22.7%), neutropenia (7.2%), and leukopenia (4.1%).

Conclusions: Pyrotinib plus vinorelbine therapy demonstrated promising effects in HER2+ MBC with tolerable toxicity, particularly in patients with second-line treatment and without prior lapatinib treatment, as well as in patients with brain metastases. Oral vinorelbine is a useful alternative to the intravenous form when combined with pyrotinib.

Clinical Trial Registration: [ClinicalTrials.gov], identifier [NCT04517305].
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http://dx.doi.org/10.3389/fonc.2021.664429DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8120312PMC
April 2021

Quantitative characterization of human oncogene promoter G-quadruplex DNA-ligand interactions using a combination of mass spectrometry and capillary electrophoresis.

Electrophoresis 2021 May 14. Epub 2021 May 14.

Department of Chemistry, University of British Columbia, Vancouver, British Columbia, Canada.

Human c-KIT oncogene is known to regulate cell growth and proliferation, and thus, acts as a probable target in the treatment of gastrointestinal tumors (GIST). To identify small molecule ligands which can specifically bind with the G-quadruplex (G4) in the c-KIT promoter region as potential antitumor agents, we propose the combination of electrospray ionization-mass spectrometry (ESI-MS), capillary electrophoresis frontal analysis (CE-FA), and Taylor dispersion analysis (TDA) to accurately investigate the G4/ligands binding properties. First, ESI-MS was used for initial screening of natural products (NPs). CE-FA was then used to calculate specific binding constants and the stoichiometry of the native state binding pair in solution. Next, TDA, a micro-capillary flow technique was used to examine the effect of the ligand binding on the diffusivity and particle size of the c-KIT G4. Two of the screened NPs, scopolamine butylbromide (L1) and isorhamnetin-3-O-neohesperidoside (L3), were found to specifically bind to the c-KIT G4 with binding constants of around 10 M and 1:1 stoichiometry in a free solution. TDA data showed that ligand binding (both L1 and L3) induced the c-KIT strands to fold into a tightly structured G4 with a decreased hydrodynamic radius. These ligands have the potential to be drug candidates for the regulation of c-KIT gene transcription by stabilizing the G4 structure. This methodology not only increased the speed of analysis but also improved its accuracy and specificity compared with the conventional binding approaches.
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http://dx.doi.org/10.1002/elps.202100077DOI Listing
May 2021

Dorsal raphe serotonergic neurons promote arousal from isoflurane anesthesia.

CNS Neurosci Ther 2021 May 11. Epub 2021 May 11.

Department of Anesthesiology and Perioperative Medicine, Xijing Hospital, Fourth Military Medical University, Xi'an, China.

Aims: General anesthesia has been widely applied in surgical or nonsurgical medical procedures, but the mechanism behind remains elusive. Because of shared neural circuits of sleep and anesthesia, whether serotonergic system, which is highly implicated in modulation of sleep and wakefulness, regulates general anesthesia as well is worth investigating.

Methods: Immunostaining and fiber photometry were used to assess the neuronal activities. Electroencephalography spectra and burst-suppression ratio (BSR) were used to measure anesthetic depth and loss or recovery of righting reflex to indicate the induction or emergence time of general anesthesia. Regulation of serotonergic system was achieved through optogenetic, chemogenetic, or pharmacological methods.

Results: We found that both Fos expression and calcium activity were significantly decreased during general anesthesia. Activation of 5-HT neurons in the dorsal raphe nucleus (DRN) decreased the depth of anesthesia and facilitated the emergence from anesthesia, and inhibition deepened the anesthesia and prolonged the emergence time. Furthermore, agonism or antagonism of 5-HT 1A or 2C receptors mimicked the effect of manipulating DRN serotonergic neurons.

Conclusion: Our results demonstrate that 5-HT neurons in the DRN play a regulative role of general anesthesia, and activation of serotonergic neurons could facilitate emergence from general anesthesia partly through 5-HT 1A and 2C receptors.
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http://dx.doi.org/10.1111/cns.13656DOI Listing
May 2021

Plasma hemoglobin and the risk of death in HIV/AIDS patients treated with antiretroviral therapy.

Aging (Albany NY) 2021 05 7;13(9):13061-13072. Epub 2021 May 7.

Department of Preventive Medicine, School of Public Health and Management, Wenzhou Medical University, Wenzhou 325035, Zhejiang, China.

Background: Previous studies concerning the effect of plasma hemoglobin (HB) and other factors that may modify the risk of death in people living with HIV/AIDS (PLHIV) treated with antiretroviral therapy (ART) are limited.

Results: Higher HB was independently linked to a lower death risk in PLHIV, with a decrease of 29% (13%, 43%) per standard deviation (SD) increment after adjusting for CD4, VL and other potential factors [hazard ratio (HR): 0.71, 95% confidence interval (CI): 0.57-0.87, P<0.001]. In addition, the addition of HB to the predictive model containing VL and CD4 significantly improved the C-index, by 0.69% (95% CI: 0.68%-0.71%), and net discrimination, by 0.5% (95% CI: 0.0%-1.6%, P=0.040), when predicting the death risk of PLHIV.

Conclusions: A lower level of HB was an independent risk factor for HIV/AIDS-associated death in PLHIV. HB combined with VL and CD4 may be an appropriate predictive model of the death risk of PLHIV.

Materials And Methods: A propensity-score matching (PSM) approach was applied to select a total of 750 PLHIV (150 deceased and 600 living) from the AIDS prevention and control information system in the Wenzhou area from 2006 to 2018. Multivariable Cox proportional hazards regression models were formulated to estimate the effect of HB. The predictive performance improvement contributed by HB was evaluated using the C-index and net reclassification improvement.
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http://dx.doi.org/10.18632/aging.202987DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8148493PMC
May 2021

MAEA is an E3 ubiquitin ligase promoting autophagy and maintenance of haematopoietic stem cells.

Nat Commun 2021 05 4;12(1):2522. Epub 2021 May 4.

Ruth L. and David S. Gottesman Institute for Stem Cell and Regenerative Medicine Research, Bronx, NY, USA.

Haematopoietic stem cells (HSCs) tightly regulate their quiescence, proliferation, and differentiation to generate blood cells during the entire lifetime. The mechanisms by which these critical activities are balanced are still unclear. Here, we report that Macrophage-Erythroblast Attacher (MAEA, also known as EMP), a receptor thus far only identified in erythroblastic island, is a membrane-associated E3 ubiquitin ligase subunit essential for HSC maintenance and lymphoid potential. Maea is highly expressed in HSCs and its deletion in mice severely impairs HSC quiescence and leads to a lethal myeloproliferative syndrome. Mechanistically, we have found that the surface expression of several haematopoietic cytokine receptors (e.g. MPL, FLT3) is stabilised in the absence of Maea, thereby prolonging their intracellular signalling. This is associated with impaired autophagy flux in HSCs but not in mature haematopoietic cells. Administration of receptor kinase inhibitor or autophagy-inducing compounds rescues the functional defects of Maea-deficient HSCs. Our results suggest that MAEA provides E3 ubiquitin ligase activity, guarding HSC function by restricting cytokine receptor signalling via autophagy.
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http://dx.doi.org/10.1038/s41467-021-22749-1DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8097058PMC
May 2021

The SARS-CoV-2 protein ORF3a inhibits fusion of autophagosomes with lysosomes.

Cell Discov 2021 May 4;7(1):31. Epub 2021 May 4.

Department of Neurosurgery, State Key Laboratory of Biotherapy, West China Hospital, Sichuan University and The Research Units of West China, Chinese Academy of Medical Sciences, Chengdu, China.

Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has caused the ongoing coronavirus disease 2019 pandemic. How SARS-CoV-2 regulates cellular responses to escape clearance by host cells is unknown. Autophagy is an intracellular lysosomal degradation pathway for the clearance of various cargoes, including viruses. Here, we systematically screened 28 viral proteins of SARS-CoV-2 and identified that ORF3a strongly inhibited autophagic flux by blocking the fusion of autophagosomes with lysosomes. ORF3a colocalized with lysosomes and interacted with VPS39, a component of the homotypic fusion and protein sorting (HOPS) complex. The ORF3a-VPS39 interaction prohibited the binding of HOPS with RAB7, which prevented the assembly of fusion machinery, leading to the accumulation of unfused autophagosomes. These results indicated the potential mechanism by which SARS-CoV-2 escapes degradation; that is, the virus interferes with autophagosome-lysosome fusion. Furthermore, our findings will facilitate strategies targeting autophagy for conferring potential protection against the spread of SARS-CoV-2.
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http://dx.doi.org/10.1038/s41421-021-00268-zDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8096138PMC
May 2021

Stabilizing Highly Active Ru Sites by Suppressing Lattice Oxygen Participation in Acidic Water Oxidation.

J Am Chem Soc 2021 May 23;143(17):6482-6490. Epub 2021 Apr 23.

Department of Electrical and Computer Engineering, University of Toronto, 10 King's College Road, Toronto, Ontario M5S 3G4, Canada.

In hydrogen production, the anodic oxygen evolution reaction (OER) limits the energy conversion efficiency and also impacts stability in proton-exchange membrane water electrolyzers. Widely used Ir-based catalysts suffer from insufficient activity, while more active Ru-based catalysts tend to dissolve under OER conditions. This has been associated with the participation of lattice oxygen (lattice oxygen oxidation mechanism (LOM)), which may lead to the collapse of the crystal structure and accelerate the leaching of active Ru species, leading to low operating stability. Here we develop Sr-Ru-Ir ternary oxide electrocatalysts that achieve high OER activity and stability in acidic electrolyte. The catalysts achieve an overpotential of 190 mV at 10 mA cm and the overpotential remains below 225 mV following 1,500 h of operation. X-ray absorption spectroscopy and O isotope-labeled online mass spectroscopy studies reveal that the participation of lattice oxygen during OER was suppressed by interactions in the Ru-O-Ir local structure, offering a picture of how stability was improved. The electronic structure of active Ru sites was modulated by Sr and Ir, optimizing the binding energetics of OER oxo-intermediates.
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http://dx.doi.org/10.1021/jacs.1c00384DOI Listing
May 2021

Olinciguat, a stimulator of soluble guanylyl cyclase, attenuates inflammation, vaso-occlusion and nephropathy in mouse models of sickle cell disease.

Br J Pharmacol 2021 Apr 17. Epub 2021 Apr 17.

Cyclerion Therapeutics Inc., Boston, Massachusetts, USA.

Background And Purpose: Reduced bioavailability of NO, a hallmark of sickle cell disease (SCD), contributes to intravascular inflammation, vasoconstriction, vaso-occlusion and organ damage observed in SCD patients. Soluble guanylyl cyclase (sGC) catalyses synthesis of cGMP in response to NO. cGMP-amplifying agents, including NO donors and phosphodiesterase 9 inhibitors, alleviate TNFα-induced inflammation in wild-type C57BL/6 mice and in 'humanised' mouse models of SCD.

Experimental Approach: Effects of the sGC stimulator olinciguat on intravascular inflammation and renal injury were studied in acute (C57BL6 and Berkeley mice) and chronic (Townes mice) mouse models of TNFα-induced and systemic inflammation associated with SCD.

Key Results: Acute treatment with olinciguat attenuated increases in plasma biomarkers of endothelial cell activation and leukocyte-endothelial cell interactions in TNFα-challenged mice. Co-treatment with hydroxyurea, an FDA-approved SCD therapeutic agent, further augmented the anti-inflammatory effect of olinciguat. In the Berkeley mouse model of TNFα-induced vaso-occlusive crisis, a single dose of olinciguat attenuated leukocyte-endothelial cell interactions, improved blood flow and prolonged survival time compared to vehicle-treated mice. In Townes SCD mice, plasma biomarkers of inflammation and endothelial cell activation were lower in olinciguat- than in vehicle-treated mice. In addition, kidney mass, water consumption, 24-h urine excretion, plasma levels of cystatin C and urinary excretion of N-acetyl-β-d-glucosaminidase and neutrophil gelatinase-associated lipocalin were lower in Townes mice treated with olinciguat than in vehicle-treated mice.

Conclusion And Implications: Our results suggest that the sGC stimulator olinciguat attenuates inflammation, vaso-occlusion and kidney injury in mouse models of SCD and systemic inflammation.
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http://dx.doi.org/10.1111/bph.15492DOI Listing
April 2021

Lateral Hypothalamic Area Glutamatergic Neurons and Their Projections to the Lateral Habenula Modulate the Anesthetic Potency of Isoflurane in Mice.

Neurosci Bull 2021 Apr 13. Epub 2021 Apr 13.

Department of Anesthesiology and Perioperative Medicine, Xijing Hospital, Fourth Military Medical University, Xi'an, 710032, China.

The lateral hypothalamic area (LHA) plays a pivotal role in regulating consciousness transition, in which orexinergic neurons, GABAergic neurons, and melanin-concentrating hormone neurons are involved. Glutamatergic neurons have a large population in the LHA, but their anesthesia-related effect has not been explored. Here, we found that genetic ablation of LHA glutamatergic neurons shortened the induction time and prolonged the recovery time of isoflurane anesthesia in mice. In contrast, chemogenetic activation of LHA glutamatergic neurons increased the time to anesthesia and decreased the time to recovery. Optogenetic activation of LHA glutamatergic neurons during the maintenance of anesthesia reduced the burst suppression pattern of the electroencephalogram (EEG) and shifted EEG features to an arousal pattern. Photostimulation of LHA glutamatergic projections to the lateral habenula (LHb) also facilitated the emergence from anesthesia and the transition of anesthesia depth to a lighter level. Collectively, LHA glutamatergic neurons and their projections to the LHb regulate anesthetic potency and EEG features.
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http://dx.doi.org/10.1007/s12264-021-00674-zDOI Listing
April 2021

Therapeutic Efficacy of Excretory-Secretory Products of Adult Worms on Sepsis-Induced Acute Lung Injury in a Mouse Model.

Front Cell Infect Microbiol 2021 24;11:653843. Epub 2021 Mar 24.

Department of Basic Medical College, Bengbu Medical College, Bengbu, China.

Acute lung injury (ALI) is a common complication of systemic inflammation or sepsis with high morbidity and mortality. Although many studies have confirmed that helminth-derived proteins had strong immunomodulatory functions and could be used to treat inflammatory diseases, there is no report on the therapeutic effect of excretory-secretory products of adult worms (-AES) on sepsis-induced ALI. In this study, the therapeutic efficacy of -AES on sepsis-induced ALI and the underlying immunological mechanism and the signaling pathway were investigated. The results indicated that after being treated with -AES, the survival rate of mice with CLP-induced sepsis was significantly increased to 50% for 72 hours after CLP surgery compared to PBS control group with all mice died. The sepsis-induced ALI was largely mitigated characterized by reduced inflammation cell infiltration and pathological changes in lung tissue, with decreased lung injury scores and lung wet/dry weight ratio. The therapeutic efficacy of -AES is associated with stimulated Tregs response with increased regulatory cytokines IL-10 and TGF-β and downregulated pro-inflammatory cytokines (TNF-α, IL-6, IL-1β). The expression of HMGB1, TLR2 and MyD88 in lung tissue was inhibited after treatment of -AES. Our results demonstrated that -AES play an important role in immunomodulation and confer a therapeutic effect on sepsis-induced ALI through inhibiting pro-inflammatory cytokines. The activation of Tregs and increased level of regulatory cytokines IL-10 and TGF-β are possibly involved in the immunomodulatory functions of -AES through HMGB1/TLR2/MyD88 signal pathway. The findings suggest -AES is a potential therapeutic agent for prevention and treatment of sepsis-induced ALI and other inflammatory diseases.
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http://dx.doi.org/10.3389/fcimb.2021.653843DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8024484PMC
March 2021

A highly selective ratiometric fluorescent probe for doxycycline based on the sensitization effect of bovine serum albumin.

J Hazard Mater 2021 Mar 26;416:125759. Epub 2021 Mar 26.

Key Laboratory of Advanced Materials of Tropical Island Resources of Ministry of Education, School of Science, Hainan University, Haikou 570228, PR China; School of Chemical Engineering and Technology, Hainan University, Haikou 570228, PR China. Electronic address:

Fluorescent probes with in-situ visual feature have received numerous attentions for detecting doxycycline (DC), a semisynthetic tetracycline antibiotic widely used in animal husbandry. However, reported fluorescent probes commonly fail to selectively detect DC among tetracycline antibiotics due to their structural similarity. In this work, bovine serum albumin-capped gold nanoclusters (BSA-AuNCs) were ingeniously used as the ratiometric fluorescent probe for detecting DC over other tetracycline antibiotics through the selective sensitization effect of BSA on DC. After adding DC, the red fluorescence of BSA-AuNCs almost remained unchanged, while the green fluorescence of DC also emerged under the sensitization of BSA. BSA-AuNCs showed the highest response toward DC among tetracycline antibiotics ascribed to the strongest sensitization effect of BSA on DC. BSA-AuNCs also displayed the features of simple synthesis, short response time (1 min) and low detection limit (36 nM). BSA-AuNCs were finally applied to detecting DC in fish samples, and further fabricated into test strips for ease of carrying. Thus, this work proposes an efficient strategy to design fluorescent probe for selectively detecting DC among tetracycline antibiotics.
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http://dx.doi.org/10.1016/j.jhazmat.2021.125759DOI Listing
March 2021

The role of Eimeria tenella EtCab protein in the attachment and invasion of host cells.

Vet Parasitol 2021 Apr 18;292:109415. Epub 2021 Mar 18.

Department of Preventive Veterinary Medicine, College of Veterinary Medicine, Shandong Agricultural University, Taian City, Shandong Province, China; Shandong Provincial Key Laboratory of Animal Biotechnology and Disease Control and Prevention, Shandong Agricultural University, Taian City, Shandong Province, China; Shandong Provincial Engineering Technology Research Center of Animal Disease Control and Prevention, Shandong Agricultural University, Taian City, Shandong Province, China. Electronic address:

Calcium-binding proteins (CaBPs) containing the specific calcium-binding motif (EF-hand) play a crucial role in important physiological events such as secretion, storage and signal transduction of cells. Recently, CaBPs have been found to be associated with host cell invasions in some parasites. In this study, an Eimeria tenella membrane-associated calcium-binding protein (EtCab) was cloned and its expression at different developmental stages, adhesive functions and host cell invasion in vitro were investigated. The results of the sequence analysis showed that EtCab contains six EF-hand motifs and the HDEL ER-retention signal belonging to the CREC (45 kDa calcium-binding protein, reticulocalbin, ER calcium-binding protein of 55 kDa, and calumenin) family. An indirect immunofluorescence assay (IFA) using specific polyclonal antibodies under permeabilized and nonpermeabilized conditions labeled EtCab on the surface of sporozoites. Quantitative real-time PCR and western blotting indicated that EtCab was highly transcribed and expressed in sporozoites. The attachment assay using a yeast surface display model showed that the adherence rates of EtCab expressed on the surfaces of yeasts to host cells were 2.5-fold greater than the control. Invasion inhibition assays revealed that specific polyclonal antibodies against EtCab significantly reduced the invasion rate of sporozoites on host cells compared to the control group (P < 0.01). These results suggest that EtCab plays an important role in the attachment and invasion of E. tenella to host cells.
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http://dx.doi.org/10.1016/j.vetpar.2021.109415DOI Listing
April 2021

Heparin-binding protein and procalcitonin in the diagnosis of pathogens causing community-acquired pneumonia in adult patients: a retrospective study.

PeerJ 2021 12;9:e11056. Epub 2021 Mar 12.

Department of Infectious Disease, Nanjing First Hospital, Nanjing Medical University, Nanjing, China.

The performance of inflammatory markers in community-acquired pneumonia (CAP) caused by different pathogens has not been fully studied. We sought to find the differences in the concentrations of procalcitonin (PCT) and heparin-binding protein (HBP) between patients with CAP caused by different pathogens. We enrolled 162 patients with CAP, divided into three groups on the basis of bacterial ( = 108), fungal ( = 21) and viral ( = 33) infection. Complete leukocyte counts and the concentration of HBP and PCT were measured, and the differences were compared with nonparametric tests. The receiver operating characteristic (ROC) curve was used to evaluate the significant differences in the sensitivity and specificity of the indicators. The leukocyte and neutrophils counts and the concentrations of HBP and PCT in the viral group were significantly lower than those in the other two groups ( < 0.001). The area under the ROC curve (AUC) of the concentration of HBP and PCT as well as leukocyte and neutrophils counts were 0.927, 0.892, 0.832 and 0.806 for distinguishing bacterial from viral infection, respectively. The best cut-off value was 20.05 ng/mL for HBP, with a sensitivity of 0.861 and specificity of 0.939. The best cut-off value was 0.195 ng/mL for PCT, with a sensitivity of 0.991 and specificity of 0.636. The best cut-off value was 5.195 × 10/L and 4.000 × 10/L for leukocyte and neutrophils counts, with sensitivity of 0.694 and 0.880 and specificity of 0.667 and 0.636, respectively. The AUC of HBP, PCT and leukocyte and neutrophil counts for distinguishing fungal from viral infection were 0.851, 0.883, 0.835 and 0.830, respectively. The best cut-off values were 29.950 ng/mL, 0.560 ng/mL, 5.265 × 10/L and 3.850 × 10/L, with sensitivity of 0.667, 0.714, 0.905 and 0.952 and specificity of 0.970, 0.879 0.667 and 0.606, respectively. There were no significant differences in the three indicators between the bacterial and fungal infection groups. The concentration of CRP showed no significant differences among the three groups. Consequently, the stronger immune response characterized by higher inflammation markers including HBP and PCT can help distinguish bacterial and fungal CAP from viral CAP.
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http://dx.doi.org/10.7717/peerj.11056DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7958890PMC
March 2021

Massage Therapy's Effectiveness on the Decoding EEG Rhythms of Left/Right Motor Imagery and Motion Execution in Patients With Skeletal Muscle Pain.

IEEE J Transl Eng Health Med 2021;9:2100320. Epub 2021 Feb 3.

Shenzhen Institute of Advanced TechnologyChinese Academy of SciencesShenzhen518055China.

Objective: Most of effectiveness assessments of the widely-used Massage therapy were based on subjective routine clinical assessment tools, such as Visual Analogue Scale (VAS) score. However, few studies demonstrated the impact of massage on the Electroencephalograph (EEG) rhythm decoding of Motor imagery (MI) and motion execution (ME) with trunk left/right bending in patients with skeletal muscle pain.

Method: We used the sample entropy (SampEn), permutation entropy (PermuEn), common spatial pattern (CSP) features, support vector machine (SVM) and logic regression (LR) classifiers. We also used the convolutional neural network (CNN) and attention-based bi-directional long short-term memory (BiLSTM) for classification.

Results: The averaged SampEn and PermuEn values of alpha rhythm decreased in almost fourteen channels for five statuses (quiet, MI with left/right bending, ME with left/right bending). It indicated that massage alleviates the pain for the patients of skeletal pain. Furthermore, compared with the SVM and LR classifiers, the BiLSTM method achieved a better area under curve (AUC) of 0.89 for the classification of MI with trunk left/right bending before massage. The AUC became smaller after massage than that before massage for the classification of MI with trunk left/right bending using CNN and BiLSTM methods. The Permutation direct indicator (PDI) score showed the significant difference for patients in different statuses (before vs after massage, and MI vs ME).

Conclusions: Massage not only affects the quiet status, but also affects the MI and ME. Clinical Impact: Massage therapy may affect a bit on the accuracy of MI with trunk left/right bending and it change the topography of MI and ME with trunk left/right bending for the patients with skeletal muscle pain.
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http://dx.doi.org/10.1109/JTEHM.2021.3056911DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7965939PMC
February 2021

SARS-CoV-2 infection of human iPSC-derived cardiac cells reflects cytopathic features in hearts of patients with COVID-19.

Sci Transl Med 2021 04 15;13(590). Epub 2021 Mar 15.

Gladstone Institutes, San Francisco, CA 94158, USA.

Although coronavirus disease 2019 (COVID-19) causes cardiac dysfunction in up to 25% of patients, its pathogenesis remains unclear. Exposure of human induced pluripotent stem cell (iPSC)-derived heart cells to severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) revealed productive infection and robust transcriptomic and morphological signatures of damage, particularly in cardiomyocytes. Transcriptomic disruption of structural genes corroborates adverse morphologic features, which included a distinct pattern of myofibrillar fragmentation and nuclear disruption. Human autopsy specimens from patients with COVID-19 reflected similar alterations, particularly sarcomeric fragmentation. These notable cytopathic features in cardiomyocytes provide insights into SARS-CoV-2-induced cardiac damage, offer a platform for discovery of potential therapeutics, and raise concerns about the long-term consequences of COVID-19 in asymptomatic and severe cases.
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http://dx.doi.org/10.1126/scitranslmed.abf7872DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8128284PMC
April 2021

Cystatin Alleviates Sepsis Through Activating Regulatory Macrophages.

Front Cell Infect Microbiol 2021 24;11:617461. Epub 2021 Feb 24.

Anhui Key Laboratory of Infection and Immunity of Bengbu Medical College, Bengbu, China.

Multi-organ failure caused by the inflammatory cytokine storm induced by severe infection is the major cause of death for sepsis. -Cys is a cysteine protease inhibitor secreted by with strong immunomodulatory functions on host immune system. Our previous studies have shown that treatment with -Cys recombinant protein (r-Cys) attenuated inflammation caused by sepsis. However, the immunological mechanism underlying the immunomodulation of -Cys for regulating inflammatory diseases is not yet known. In this study, we investigated the effect of -Cys on the macrophage M2 polarization and subsequent therapeutic effect on sepsis. The r-Cys was expressed in yeast . Incubation of mouse bone marrow-derived macrophages (BMDMs) with yeast-expressed r-Cys significantly activated the polarization of macrophages to M2 subtype characterized by the expression of F4/80 CD206 with the elated secretion of IL-10 and TGF-β. Adoptive transfer of r-Cys treated BMDMs to mice with sepsis induced by cecal ligation and puncture (CLP) significantly improved their survival rates and the systemic clinical manifestations of sepsis compared with mice receiving non-treated normal BMDMs. The therapeutic effect of -Cys-induced M2 macrophages on sepsis was also reflected by the reduced pathological damages in organs of heart, lung, liver and kidney and reduced serological levels of tissue damage-related ALT, AST, BUN and Cr, associated with downregulated pro-inflammatory cytokines (IFN-gamma and IL-6) and upregulated regulatory anti-inflammatory cytokines (IL-10 and TGF-β). Our results demonstrated that -Cys is a strong immunomodulatory protein with anti-inflammatory features through activating M2 macrophage polarization. The findings of this study suggested that -Cys itself or -Cys-induced M2 macrophages could be used as therapeutic agents in the treatment of sepsis or other inflammatory diseases.
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http://dx.doi.org/10.3389/fcimb.2021.617461DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7943722PMC
February 2021

Exploration of the Prognostic and Immunotherapeutic Value of B and T Lymphocyte Attenuator in Skin Cutaneous Melanoma.

Front Oncol 2020 26;10:592811. Epub 2021 Feb 26.

Department of Thyroid and Breast Surgery, The First Affiliated Hospital of Wenzhou Medical University, Wenzhou, China.

B and T lymphocyte attenuator (BTLA) is a newly identified immune checkpoint molecular belonging to the CD28 immunoglobulin superfamily. However, the expression and clinical value of BTLA in skin cutaneous melanoma (SKCM) has not been widely characterized. We found that BTLA levels were upregulated in metastatic melanoma compared to normal skin tissues and primary melanoma. Higher BTLA was also correlated with improved prognosis in SKCM based on several datasets. The multivariate Cox regression model revealed that BTLA was an independent survival indicator in metastatic melanoma. Tumor microenvironment analysis indicated BTLA was positively associated with the infiltrating levels of different immune cells and the activity of the anti-cancer immunity cycle. Importantly, BTLA accurately predicted the outcome of melanoma patients treated with MAGE-A3 blocker or first-line anti-PD-1. The present findings disclose that BTLA is a reliable biomarker for prognosis and immunotherapeutic response and might contribute to developing novel SKCM immunological treatment strategies.
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http://dx.doi.org/10.3389/fonc.2020.592811DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7953043PMC
February 2021

riboCIRC: a comprehensive database of translatable circRNAs.

Genome Biol 2021 Mar 8;22(1):79. Epub 2021 Mar 8.

State Key Laboratory of Ophthalmology, Zhongshan Ophthalmic Center, Sun Yat-sen University, Guangzhou, China.

riboCIRC is a translatome data-oriented circRNA database specifically designed for hosting, exploring, analyzing, and visualizing translatable circRNAs from multi-species. The database provides a comprehensive repository of computationally predicted ribosome-associated circRNAs; a manually curated collection of experimentally verified translated circRNAs; an evaluation of cross-species conservation of translatable circRNAs; a systematic de novo annotation of putative circRNA-encoded peptides, including sequence, structure, and function; and a genome browser to visualize the context-specific occupant footprints of circRNAs. It represents a valuable resource for the circRNA research community and is publicly available at http://www.ribocirc.com .
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http://dx.doi.org/10.1186/s13059-021-02300-7DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7938571PMC
March 2021

Mitochondrial fission is a critical modulator of mutant APP-induced neural toxicity.

J Biol Chem 2021 Feb 24:100469. Epub 2021 Feb 24.

Gladstone Institute of Neurological Disease, San Francisco, CA, 94158; Department of Neurology, University of California, San Francisco, San Francisco, California, 94158; Graduate Programs in Neuroscience and Biomedical Sciences, University of California, San Francisco, San Francisco, California, 94158. Electronic address:

Alterations in mitochondrial fission may contribute to the pathophysiology of several neurodegenerative diseases, including Alzheimer's disease (AD). However, we understand very little about the normal functions of fission, or how fission disruption may interact with AD-associated proteins to modulate pathogenesis. Here we show that loss of the central mitochondrial fission protein dynamin-related 1 (Drp1) in CA1 and other forebrain neurons markedly worsens the learning and memory of mice expressing mutant human amyloid-precursor protein (hAPP) in neurons. In cultured neurons, Drp1KO and hAPP converge to produce mitochondrial Ca (mitoCa) overload, despite decreasing mitochondria-associated ER membranes (MAMs) and cytosolic Ca. This mitoCa overload occurs independently of ATP levels. These findings reveal a potential mechanism by which mitochondrial fission protects against hAPP-driven pathology.
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http://dx.doi.org/10.1016/j.jbc.2021.100469DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8042169PMC
February 2021

Human papillomavirus infection rate, distribution characteristics, and risk of age in pre- and postmenopausal women.

BMC Womens Health 2021 02 25;21(1):80. Epub 2021 Feb 25.

Healthcare Department, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China.

Background: The incidence rate of cervical cancer is increasing yearly. The persistent infection of high-risk human papillomavirus (HPV) is the main factor leading to cervical cancer. HPV infection is double peak type. This study aimed at analyzing the HPV distribution characteristics, infection rate, and risk of age in pre- and postmenopausal women. So as to provide reference for the prevention of HPV infection and cervical cancer screening strategy.

Methods: A retrospective analysis of 4614 women who underwent cervical cytology, and HPV examination from January 2018 to October 2019 at the healthcare department of Wuhan Union Hospital was done. We explored the characteristics and distribution of HPV infections around the menopause, then comparing the infection rate of HPV in postmenopause and over 65 years old, in order to analyze the influence of different ages on HPV infection.

Results: Generally, the HPV infection rate was 13.10% (539/4115), whereby the high-risk subtype constituted 73.84% (398/539) of all positive cases. On the other hand, the HPV39 infection was more common in postmenopausal women; however, there was no significant difference in the distribution of the other types in the pre- and postmenopausal women. The first four types were 52/53/58/16. The results further showed that the rates of HPV infection before and after menopause were 12.34% (367/2975) and 15.09% (172/1140), respectively, which had no significant difference (P = 0.056), but more susceptible to high-risk HPV infection after the age of 65 (P = 0.041). Except for 40 years old to menopause, the infection rate of high-risk HPV in this age group was different from that in postmenopause (P = 0.023, 0.729 (0.555, 0.957)), other age groups had no significant effect on high-risk HPV infection.

Conclusions: It was concluded that whether menopause has nothing to do with HPV infection. Moreover, the risk of high-risk HPV infection in women aged 40 to premenopausal is relatively low, but the infection rate increases after 65. Hence the cutoff screening age should be appropriately prolonged.
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http://dx.doi.org/10.1186/s12905-021-01217-4DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7905912PMC
February 2021

Lateral hypothalamus orexinergic inputs to lateral habenula modulate maladaptation after social defeat stress.

Neurobiol Stress 2021 May 30;14:100298. Epub 2021 Jan 30.

Department of Anesthesiology and Perioperative Medicine, Xijing Hospital, Xi'an, Shaanxi, China.

Social stress, a common stressor, causes multiple forms of physical and mental dysfunction. Prolonged exposure to social stress is associated with a higher risk of psychological disorders, including anxiety disorders and major depressive disorder (MDD). The orexinergic system is involved in the regulation of multiple motivated behaviors. The current study examined the regulatory effect of orexinergic projections from the lateral hypothalamic area (LHA) to the lateral habenula (LHb) in depression- and anxiety-like behaviors after chronic social defeat stress. When mice were defeated during social interaction, both orexinergic neurons in the LHA and glutamatergic neurons in the LHb were strongly activated, as indicated by the FosTRAP strategy. Infusion of orexin in the LHb significantly alleviated social avoidance and depression-like behaviors induced by chronic social defeat stress. Administration of an orexin receptor 2 antagonist in the LHb further aggravated the depressive phenotype. Photoactivation of orexinergic cell bodies in the LHA or terminals in the LHb relieved anxiety-like behaviors induced by chronic social defeat stress. Collectively, we identified the antidepressant and anxiolytic effects of the circuit from LHA orexinergic neurons to the LHb in response to chronic social stress, providing new evidence of the antidepressant properties of LHA orexin circuits.
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http://dx.doi.org/10.1016/j.ynstr.2021.100298DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7859368PMC
May 2021

A Soft Wearable and Fully-Textile Piezoresistive Sensor for Plantar Pressure Capturing.

Micromachines (Basel) 2021 Jan 22;12(2). Epub 2021 Jan 22.

Shenzhen Key Laboratory for Low-cost Healthcare, Shenzhen Institutes of Advanced Technology, Chinese Academy of Sciences, 1068 Xueyuan Avenue, Shenzhen University Town, Shenzhen 518055, China.

The trends of wearable health monitoring systems have led to growing demands for gait-capturing devices. However, comfortability and durability under repeated stress are still challenging to achieve in existing sensor-enabled footwear. Herein, a flexible textile piezoresistive sensor (TPRS) consisting of a reduced graphene oxide (rGO)-cotton) fabric electrode and an Ag fabric circuit electrode is proposed. Based on the mechanical and electrical properties of the two fabric electrodes, the TPRS exhibits superior sensing performance, with a high sensitivity of 3.96 kPa-1 in the lower pressure range of 0-36 kPa, wide force range (0-100 kPa), fast response time (170 ms), remarkable durability stability (1000 cycles) and detection ability in different pressures ranges. For the prac-tical application of capturing plantar pressure, six TPRSs were mounted on a flexible printed circuit board and integrated into an insole. The dynamic plantar pressure distribution during walking was derived in the form of pressure maps. The proposed fully-textile piezoresistive sensor is a strong candidate for next-generation plantar pressure wearable monitoring devices.
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http://dx.doi.org/10.3390/mi12020110DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7926843PMC
January 2021

Re. "ω-6/ω-3 fatty acid ratio as an essential predictive biomarker in the management of type 2 diabetes mellitus".

Nutrition 2020 Dec 17:111111. Epub 2020 Dec 17.

Division of Epidemiology and Biostatistics, Department of Preventive Medicine, School of Public Health & Management, Wenzhou Medical University, Wenzhou, Zhejiang, China.

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http://dx.doi.org/10.1016/j.nut.2020.111111DOI Listing
December 2020

Phenotypic and genetic characteristics of isolates from site-specific dental plaque in China.

J Med Microbiol 2021 Mar 18;70(3). Epub 2021 Jan 18.

Department of Microbiology and Anhui Key Laboratory of Infection and Immunity, Bengbu Medical College, Bengbu, PR China.

is an important cariogenic microbe. The potential characteristics of isolates from site-specific dental plaque are still not clear. This study aimed to investigate the phenotypic and genetic characteristics of isolates from site-specific dental plaque in China. We used isolated from children with early-childhood caries (ECC) and caries-free children to compare the phenotypic and genetic characteristics of from site-specific dental plaque samples. The ECC subjects presented two sites: a cavitated lesion and a sound surface. The caries-free subjects presented one sound surface. Growth pattern, biofilm, decrease in pH, extracellular polysaccharide, expression levels of virulence-related genes, multilocus sequence typing (MLST) and phylogenetic trees were evaluated among these three sites. The phenotypes detected between the cavitated and sound surfaces of ECC children were similar. However, the capacity for biofilm formation, pH drop and expression levels of genes ( and ) of in the caries-free group were lower compared with those of the ECC group. We identified 44 new alleles and 77 new sequence types. More than 90 % of the children with ECC shared an identical sequence type. The distribution of sequence types among different subjects showed diversity, and child-to-child transmission was detected. This is the first report of MLST on site-specific dental plaques in a single subject, and indicates that isolated from site-specific dental plaque of a single subject showed similar phenotypes as a result of the isolates were closely related.
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http://dx.doi.org/10.1099/jmm.0.001313DOI Listing
March 2021

Chitosan-riboflavin composite film based on photodynamic inactivation technology for antibacterial food packaging.

Int J Biol Macromol 2021 Mar 13;172:231-240. Epub 2021 Jan 13.

College of Food Science and Technology, Shanghai Ocean University, Shanghai 201306, China; Shanghai Engineering Research Center of Aquatic-Product Processing & Preservation, Shanghai 201306, China; Laboratory of Quality & Safety Risk Assessment for Aquatic Products on Storage and Preservation (Shanghai), Ministry of Agriculture and Rural Affairs, Shanghai 201306, China; Engineering Research Center of Food Thermal-processing Technology, Shanghai Ocean University, Shanghai 201306, China. Electronic address:

Photodynamic inactivation (PDI) is a novel sterilization technology that has proven effective in medicine. This study focused on applying PDI to food packaging, where chitosan (CS) films containing photosensitizing riboflavin (RB) were prepared via solution casting. The CS-RB composite films exhibited good ultraviolet (UV)-barrier properties, and had a visually appealing highly transparent yellow appearance. Scanning electron microscopy (SEM) confirmed even dispersion of RB throughout the CS film. The addition of RB led to improved film characteristics, including the thickness, mechanical properties, solubility, and water barrier properties. The CS-RB composite films produced sufficient singlet oxygen under blue LED irradiation for 2 h to inactivate two food-borne pathogens (Listeria monocytogenes and Vibrio parahaemolyticus) and one spoilage bacteria (Shewanella baltica). The CS-RB composite films were assessed as a salmon packaging material, where inhibition of bacterial growth was observed. The film is biodegradable, and has the potential to alleviate the issues associated with the excessive use of petrochemical materials, such as environmental pollution and limited resources. The CS-RB composite films showed potential as a novel environmentally friendly packaging material for shelf-life extension of refrigerated food products.
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http://dx.doi.org/10.1016/j.ijbiomac.2021.01.056DOI Listing
March 2021

Lactate as a metabolite from probiotic Lactobacilli mitigates ethanol-induced gastric mucosal injury: an in vivo study.

BMC Complement Med Ther 2021 Jan 11;21(1):26. Epub 2021 Jan 11.

Wenzhou Key Laboratory of Sanitary Microbiology, Key Laboratory of Laboratory Medicine, Ministry of Education, China, School of Laboratory Medicine and Life Sciences, Wenzhou Medical University, Wenzhou, 325035, China.

Background: Pre-administration of probiotic Lactobacilli attenuates ethanol-induced gastric mucosal injury (GMI). The underpinning mechanisms remain to be elucidated. We speculated that lactate, the main metabolite of Lactobacillus that can be safely used as a common food additive, mediated the gastroprotective effect. This study aimed to gain experimental evidence to support our hypothesis and to shed lights on its underlying mechanisms.

Methods: Lactate was orally administrated to mice at different doses 30 min prior to the induction of GMI. Gastric tissue samples were collected and underwent histopathological and immunohistochemical assessments, enzyme-linked immunosorbent assay, quantitative polymerase chain reaction (qPCR) and western blot analyses.

Results: Pretreatment with lactate at 1-3 g/kg significantly curtailed the severity of ethanol-induced GMI, as shown by morphological and histopathological examinations of gastric tissue samples. Significantly lower level of cytokines indicative of local inflammation were found in mice receiving lactate treatment prior to ethanol administration. Western-blot, immunohistochemical analysis and qPCR suggested that gastroprotective properties of lactate were mediated by its modulatory effects on the expression of the apoptosis regulator gene Bax, the apoptotic executive protein gene Casp3, and genes critical for gastric mucosal integrity, including those encoding tight junction proteins Occludin, Claudin-1, Claudin-5, and that for lactate receptor GPR81.

Conclusion: Lactate mitigates ethanol-induced GMI by curtailing local gastric inflammatory response, down-regulating the expression of the apoptosis regulator and executor genes Bax and Casp3, and up-regulating the expression of genes encoding tight junction proteins Occludin, Claudin-1, and Claudin-5 and the lactate receptor GPR81.
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http://dx.doi.org/10.1186/s12906-020-03198-7DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7802211PMC
January 2021

IL-17 Affects the Progression, Metastasis, and Recurrence of Laryngeal Cancer the Inhibition of Apoptosis through Activation of the PI3K/AKT/FAS/FASL Pathways.

J Immunol Res 2020 18;2020:2953191. Epub 2020 Dec 18.

Department of Otorhinolaryngology, The Second Hospital of Anhui Medical University, Hefei, Anhui 230601, China.

Background: Cytokines play important roles in the development and prognosis of laryngeal cancer (LC). Interleukin-17 (IL-17) from a distinct subset of CD4+ T cells may significantly induce cancer-elicited inflammation to prevent tumor immune surveillance.

Methods: The expression levels of IL-17 were examined among 60 patients with LC. Immunofluorescence colocalization experiments were performed to verify the localization of IL-17 and FAS/FASL in Hep-2 and Tu212 cells. The role of IL-17 was determined using techniques in the LC cell line.

Results: In the LC patients, cytokines were dysregulated in LC tissues compared with normal tissues. It was found that IL-17 was overexpressed in a cohort of 60 LC tumors paired with nontumor tissues. Moreover, high IL-17 expression was significantly associated with the advanced T category, the late clinical stage, differentiation, lymph node metastasis, and recurrence. In addition, the time course expression of FAS and FASL was observed after stimulation and treatment with the IL-17 stimulator. Finally, experiments demonstrated that IL-17 functioned as an oncogene by inhibiting the apoptosis of LC cells the PI3K/AKT/FAS/FASL pathways.

Conclusions: In summary, these findings demonstrated for the first time the role of IL-17 as a tumor promoter and a prometastatic factor in LC and indicated that IL-17 may have an oncogenic role and serve as a potential prognostic biomarker and therapeutic target in LC.
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http://dx.doi.org/10.1155/2020/2953191DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7769679PMC
December 2020

Differentiation of Electric Response in Highly Oriented Regioregular Poly(3-hexylthiophene) under Anisotropic Strain.

ACS Appl Mater Interfaces 2021 Jan 7;13(2):2944-2951. Epub 2021 Jan 7.

State Key Laboratory of Chemical Resource Engineering, College of Materials Science and Engineering, Beijing University of Chemical Technology, Beijing 100029, China.

Exploring flexible and stretchable conjugated polymer devices has garnered particular attention. This work provides a new technology to improve the electrical properties in a stretching process by skillfully assisting the anisotropic tensile properties of oriented regioregular poly(3-hexylthiophene) (P3HT) films. Oriented P3HT films with a long-range ordered chain alignment are fabricated, and stretchable conducting films are achieved by laminating oriented P3HT films and polydimethylsiloxane (PDMS) layers. The differentiation of electrical response is identified when the film is under different stretching directions. The electrical stability of the P3HT film during the stretching process is much better when the stretching direction is perpendicular than along the -axis of the P3HT film. Moreover, the multiscale structure evolution of P3HT films under stretching is explored. The technology based on oriented conductive polymers under anisotropic stretching condition provides not only a new strategy for fabricating high-quality stretchable devices but also theoretical guidance for studying the mechanical properties for the aligned conjugated film.
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http://dx.doi.org/10.1021/acsami.0c19199DOI Listing
January 2021