Publications by authors named "Hui-Qin Gao"

3 Publications

  • Page 1 of 1

[Effect of swertiamarin, gentiopicrin and sweroside on cell apoptosis and expression of Bcl-2 in rheumatoid arthritis fibroblast-like synoviocytes].

Zhongguo Zhong Yao Za Zhi 2021 Jan;46(2):406-411

School of Pharmacy, Gansu University of Chinese Medicine Lanzhou 730000, China.

The aim of this paper was to discuss the effect of swertiamarin, gentiopicrin and sweroside on rheumatoid arthritis fibroblast-like synoviocytes(RA-FLSs) and B-cell lymphoma-2(Bcl-2) and their mechanisms. ZINC database and RCSB PDB database were retrieved for 3 D chemical structures of swertiamarin, gentiopicrin and sweroside and 3 D target protein structures. AutoDock Mgltools 1.5.6, AutoDockVina 1.1.2 and pyMOL 2.2.0 were applied for molecular docking to analyze the relationship between Bcl-2(1 GJH) target protein and important ingredients. The cell apoptosis of RA-FLSs was tested by Annexin V-FITC. The Bcl-2 protein expression of RA-FLSs treated with different ingredients was tested by Western blot. The Bcl-2 mRNA expression of RA-FLSs treated with different ingredients was tested by RT-PCR. Swertiamarin, gentiopicrin and sweroside were docked well with Bcl-2(1 GJH). The binding energy of swertiamarin was-6.9 kcal·mol~(-1), the binding energy of gentiopicrin was-6.7 kcal·mol~(-1) and the binding energy of sweroside was-6.4 kcal·mol~(-1). Compared with the blank group, the Bcl-2 protein expression of each group were reduced, while that of the gentiopicrin group was the highest(P<0.01). Compared with the blank group, the Bcl-2 mRNA expression of each groups were reduced. Gentiopicrin can reduce the Bcl-2 protein expression and the Bcl-2 mRNA expression, so as to promote the RA-FLSs apoptosis.
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http://dx.doi.org/10.19540/j.cnki.cjcmm.20200927.401DOI Listing
January 2021

A new abietane diterpene and anti-complementary constituents from .

Nat Prod Res 2020 Jan 5:1-8. Epub 2020 Jan 5.

School of Pharmacy, Institutes of Integrative Medicine, Fudan University, Shanghai, China.

Anti-complementary activity-guided fractionation led to the isolation of a new abietane diterpene () and twenty-five known compounds () from the twigs and leaves of . All the compounds were isolated from for the first time. The structure of was assigned by spectroscopic data and X-ray crystallography analysis. Five lignans (, , , and ), two flavones ( and ), and one coumarin () exhibited anti-complementary activity with CH values ranging from 0.3 to 3.69 mM.
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http://dx.doi.org/10.1080/14786419.2019.1709191DOI Listing
January 2020

[Research progress on pharmacological action and clinical application of Stephania Tetrandrae Radix].

Zhongguo Zhong Yao Za Zhi 2017 Feb;42(4):634-639

Gansu University of Traditional Chinese Medicine, Lanzhou 730000, China.

Stephania Tetrandrae Radix is one of the common traditional Chinese medicines, which has bitter and pungent taste as well as cold properties. It can subside edema, get rid of rheumatism and relieve pain. Therefore, it is mainly used for the treatment of rheumatism arthralgia, edema, dysuria, athlete's foot, swollen wet sores and other diseases in traditional Chinese medicine(TCM). Stephania Tetrandrae Radix is mainly composed of dual-benzyl isoquinoline alkaloids, including tetrandrine, fangchinoline and so on. Modern pharmacology research shows that Stephania Tetrandrae Radix and its main components have a wide range of pharmacological activities in the anti-inflammatory, anti-pathogenic microorganisms, anti-tumor, anti-hypertensive, anti-arrhythmic, anti-myocardial ischemia, anti-fibrosis, anti-silicosis, inhibiting scar and other aspects, with broad application prospect. Stephania Tetrandrae Radix is often applied with compatibility of other Chinese medicines in clinically, and has achieved obvious effects in the treatment of rheumatoid arthritis, cardiovascular disease, cancer, hypertension, liver ascites and other diseases. There are some representative prescriptions, such as Fangji Fuling decoction, Fangji huangqi decoction, Jijiao Lizhuang pill, Xuanbi decoction, and compound Hanfangji granule. In this paper, the pharmacological effects and clinical applications of Stephania Tetrandrae Radix in the past ten years were reviewed, providing reference for its further development and application.
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http://dx.doi.org/10.19540/j.cnki.cjcmm.20170121.024DOI Listing
February 2017