Publications by authors named "Hui Zhu"

1,064 Publications

  • Page 1 of 1

Treatment for Flexible Flatfoot in Children With Subtalar Arthroereisis and Soft Tissue Procedures.

Front Pediatr 2021 21;9:656178. Epub 2021 May 21.

Department of Orthopedics, Shanghai Tongji Hospital, Tongji University School of Medicine, Shanghai, China.

Children with flexible flatfoot is common in clinics and there is no unified conclusion on surgical treatment. And for some patients with severe deformities, the correction of the subtalar joint arthroereisis combine the release of the Achilles tendon or gastrocnemius muscle release is still not satisfactory. The main aim of the present study was to investigate the therapeutic outcomes of subtalar arthroereisis combined with Achilles tendon or gastrocnemius recession and medial soft tissue (spring ligament, talonavicular joint capsule, tibionavicular ligaments and tibiospring ligaments) tightening for treating flexible flatfoot with severe deformities. Thirty patients (32 feet) with pediatric flexible flatfoot who underwent subtalar arthroereisis and soft tissue procedures during January 2016 to January 2018. There were 18 males (20 feet) and 12 females (12 feet) with an average age of 9.5 years (range, 8-12 years). We used the AOFAS scores and VAS scores combined with angles measure to evaluate the pre-operative and post-operative status. Thirty patients (32 feet) were followed up for 25.3 months on average (range, 18-36 months). There was no infection. Post-operative foot pain, arch collapse, and other symptoms improved. At last follow-up, the Meary angle was decreased from 17.5° ± 4.4° to 4.1° ± 1.2° ( < 0.05), the talar-first metatarsal (AP) was decreased from 15.3° ± 3.1° to 4.8° ± 1.3°( < 0.05), The mean AOFAS score was rose from 66.6 ± 5.8 to 88.6 ± 7.9 ( < 0.05), the mean VAS score was decreased from 6.6 ± 0.6 to 1.7 ± 0.3 ( < 0.05). The subtalar arthroereisis combined with soft tissue procedures can effectively correct flexible flatfoot in children and it is a significant method for severe forefoot abduction reconstruction. IV.
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http://dx.doi.org/10.3389/fped.2021.656178DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8175848PMC
May 2021

Water reuse and growth inhibition mechanisms for cultivation of microalga Euglena gracilis.

Biotechnol Biofuels 2021 Jun 5;14(1):132. Epub 2021 Jun 5.

Shenzhen Key Laboratory of Marine Bioresource and Eco-Environmental Science, Shenzhen Engineering Laboratory for Marine Algal Biotechnology, Guangdong Provincial Key Laboratory for Plant Epigenetics, College of Life Sciences and Oceanography, Shenzhen University, Shenzhen, 518060, China.

Background: Microalgae can contribute to more than 40% of global primary biomass production and are suitable candidates for various biotechnology applications such as food, feed products, drugs, fuels, and wastewater treatment. However, the primary limitation for large-scale algae production is the fact that algae requires large amounts of fresh water for cultivation. To address this issue, scientists around the world are working on ways to reuse the water to grow microalgae so that it can be grown in successive cycles without the need for fresh water.

Results: In this study, we present the results when we cultivate microalgae with cultivation water that is purified and reused. Specifically, we purify the cultivation water using an ultrafiltration membrane (UFM) treatment and investigate how this treatment affects: the biomass and biochemical components of the microalgae; characteristics of microalgae growth inhibitors; the mechanism whereby potential growth inhibitors are secreted (followed using metabolomics analysis); the effect of activated carbon (AC) treatment and advanced oxidation processes (AOPs) on the removal of growth inhibitors of Euglena gracilis. Firstly, the results show that E. gracilis can be only cultivated through two growth cycles with water that has been filtered and reused, and the growth of E. gracilis is significantly inhibited when the water is used a third time. Secondly, as the number of reused water cycles increases, the Cl concentration gradually increases in the cultivation water. When the Cl concentration accumulates to a level of fivefold higher than that of the control, growth of E. gracilis is inhibited as the osmolality tolerance range is exceeded. Interestingly, the osmolality of the reused water can be reduced by replacing NHCl with urea as the source of nitrogen in the cultivation water. Thirdly, E. gracilis secretes humic acid (HA)-which is produced by the metabolic pathways for valine, leucine, and isoleucine biosynthesis and by linoleic acid metabolism-into the cultivation water. Because HA contains large fluorescent functional groups, specifically extended π(pi)-systems containing C=C and C=O groups and aromatic rings, we were able to observe a positive correlation between HA concentration and the rate of inhibition of E. gracilis growth using fluorescence spectroscopy. Moreover, photosynthetic efficiency is adversely interfered by HA, thereby reductions in the synthetic efficiency of paramylon and lipid in E. gracilis. In this way, we are able to confirm that HA is the main growth inhibitor of E. gracilis. Finally, we verify that all the HA is removed or converted into nutrients efficiently by AC or UV/HO/O treatments, respectively. As a result of these treatments, growth of E. gracilis is restored (AC treatment) and the amount of biomass is promoted (UV/HO/O treatment).

Conclusions: These studies have important practical and theoretical significance for the cyclic cultivation of E. gracilis and for saving water resources. Our work may also provide a useful reference for other microalgae cultivation.
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http://dx.doi.org/10.1186/s13068-021-01980-4DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8180174PMC
June 2021

Downregulation of lncRNA XR_429159.1 Linked to Brain Metastasis in Patients With Limited-Stage Small-Cell Lung Cancer.

Front Oncol 2021 17;11:603271. Epub 2021 May 17.

Department of Oncology, Renmin Hospital of Wuhan University, Wuhan, China.

The purpose of this study was to identify aberrant long non-coding RNAs (lncRNAs) and explore the predictive value of lncRNA expression patterns on the risk of brain metastases (BMs) in patients with limited-stage small-cell lung cancer (SCLC). We executed an array of lncRNA and mRNA chip assays to examine the expression in peripheral blood mononuclear cells obtained from SCLC patients with BMs and compared the expression patterns against those from patients without BMs to identify lncRNAs associated with BMs. Validation was performed against clinical data to further confirm the relationship between lncRNAs and BM. Kaplan-Meier analysis was applied to estimate the cumulative incidence of BMs, and differences between the groups were analyzed using the log-rank test. The expression of 67 lncRNAs (27 upregulated and 40 downregulated) and 47 mRNAs (20 upregulated and 27 downregulated) was significantly different between the BM and non-BM groups (fold change ≥ 2.0, -value ≤ 0.05), based on the lncRNA and mRNA chip assays. Four lncRNAs were verified by quantitative real-time polymerase chain reaction (qRT-PCR) to confirm the accuracy of the microarray data, and the results of 11 patient pairs (11 patients with BM and 11 patients without BM) revealed that low LncRNA XR_429159.1 expression was a high-risk factor for BM. Further clinical data showed that the incidence of BM among 25 patients with low-level LncRNA XR_429159.1 expression was 31% at 1 year, compared with 14.3% among the 18 patients with high-level LncRNA XR_429159.1 expression ( = 0.035). Our present study identified the low-level expression of lncRNA XR_429159.1 as a high-risk factor among BM in patients with limited-stage SCLC. LncRNA XR_429159.1 is a critical molecule that regulates SCLC metastasis, involved in the neuroepithelial transforming gene 1 (NET1) pathway, and serum levels of this lncRNA are significantly associated with the risk of BM.
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http://dx.doi.org/10.3389/fonc.2021.603271DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8166247PMC
May 2021

Occurrence of Phytoplasma Belonging to 16SrII Group Associated with Witches'-broom Symptoms in Emilia sonchifolia in Hainan Island of China.

Plant Dis 2021 Jun 2. Epub 2021 Jun 2.

Coconut Research Institute, Plant Protection, Wenqing Avenue, NO.496, Coconut Research Institute, Wenchang City, Hainan Province, China, 571339;

Emilia sonchifolia is a medical plant belonging to the family of Asteraceae, mainly used as a traditional Chinese medicine with the function of anti-inflammatory, analgesic, antibacterial and so on. During October to November 2020, the plants showing abnormal symptoms including witches'-broom, internode shortening, leaf chlorosis and leaflet were found in Hainan province, a tropical island of China. The total DNA of the plant samples were extracted using 0.10 g fresh plant leaves using CTAB method. PCR reactions were performed using primers R16mF2/R16mR1 and secAfor1/secArev3 specific for phytoplasma 16S rRNA and secA gene fragments. The target productions of the two gene fragments of phytoplasma were detected in the DNA from three symptomatic plant samples whereas not in the DNA from the symptomless plant samples. The two gene fragments of the DNA extracted from the symptomatic plant samples were all identical, with the length of 1324 bp 16S rRNA and 760 bp secA gene sequence fragments, putatively encoding 253 (secA) amino acids sequence. The phytoplasma strain was named as Emilia sonchifolia witches'-broom (EsWB) phytoplasma, EsWB-hnda strain. To our knowledge, this was the first report that Emilia sonchifolia witches'-broom disease was caused by the phytoplasma belonging to16SrII-V subgroup in Hainan island of China, with close relationship to 16SrII peanut witches'-broom group phytoplasma strains infecting the plants like peanut, Desmodium ovalifolium and cleome from the same island of China and cassava from Viet Nam.
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http://dx.doi.org/10.1094/PDIS-01-21-0043-PDNDOI Listing
June 2021

The diagnostic and predictive efficacy of F-FDG PET/CT metabolic parameters for EGFR mutation status in non-small-cell lung cancer: A meta-analysis.

Eur J Radiol 2021 May 26;141:109792. Epub 2021 May 26.

Department of Radiology, Beijing Friendship Hospital, Capital Medical University, No.95 Yongan Road, West District, Beijing 100050, PR China. Electronic address:

Purpose: To investigate the predictive performance of the maximum standardized uptake value (SUV) and mean standardized uptake value (SUV) of primary lesions based on 18 F-fluorodeoxyglucose positron emission tomography/computed tomography (F-FDG PET/CT) for EGFR mutation status in patients with non-small cell lung cancer (NSCLC).

Methods: The PubMed/Medline, Embase, Cochrane Library and Web of Science databases were searched as of January 1, 2021. Studies whose reported data could be used to construct contingency tables were included. Study characteristics were extracted, and methodological quality assessment was conducted by two separate reviewers using the Quality Assessment of Diagnostic Accuracy Studies. The pooled sensitivity, specificity and area under the summary receiver operating characteristic curve (AUROC) were calculated. The possible causes of heterogeneity were analysed by meta-regression.

Results: The 18 included studies had a total of 4024 patients. The majority of the studies showed a low to unclear risk of bias and concerns of applicability. For differentiating EGFR-mutant NSCLC from wild-type NSCLC, the pooled sensitivity and specificity were 71 % and 60 % for SUV and 64 % and 63 % for SUV, respectively. The summary AUROCs of SUV and SUV were 0.69 (95 % CI, 0.65-0.73) and 0.68 (95 % CI, 0.64-0.72), respectively. The meta-regression analysis indicated that blindness to EGFR mutation test results, the number of readers and the number of PET/CT scanners were possible causes of heterogeneity.

Conclusions: Our meta-analysis implied that SUV and SUV of primary lesions from F-FDG PET/CT harboured moderate predictive efficacy for the EGFR mutation status of NSCLC.
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http://dx.doi.org/10.1016/j.ejrad.2021.109792DOI Listing
May 2021

Mesenchymal Stem Cells in Preclinical Infertility Cytotherapy: A Retrospective Review.

Stem Cells Int 2021 17;2021:8882368. Epub 2021 May 17.

First Affiliated Hospital, Heilongjiang University of Chinese Medicine, Harbin, Heilongjiang, China.

Infertility is a global reproductive disorder which is caused by a variety of complex diseases. Infertility affects the individual, family, and community through physical, psychological, social and economic consequences. The results from recent preclinical studies regarding stem cell-based therapies are promising. Stem cell-based therapies cast a new hope for infertility treatment as a replacement or regeneration strategy. The main features and application prospects of mesenchymal stem cells in the future of infertility should be understood by clinicians. Mesenchymal stem cells (MSCs) are multipotent stem cells with abundant source, active proliferation, and multidirectional differentiation potential. MSCs play a role through cell homing, secretion of active factors, and participation in immune regulation. Another advantage is that, compared with embryonic stem cells, there are fewer ethical factors involved in the application of MSCs. However, a number of questions remain to be answered prior to safe and effective clinical application. In this review, we summarized the recent status of MSCs in the application of the diseases related to or may cause to infertility and suggest a possible direction for future cytotherapy to infertility.
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http://dx.doi.org/10.1155/2021/8882368DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8143877PMC
May 2021

Vitamin D stimulates miR-26b-5p to inhibit placental COX-2 expression in preeclampsia.

Sci Rep 2021 May 27;11(1):11168. Epub 2021 May 27.

Department of Physiology, Harbin Medical University, Harbin, 150081, China.

Vitamin D insufficiency or deficiency during pregnancy has been associated with an increased risk of preeclampsia. Increased placental cyclooxygenase-2 (COX-2) activity was proposed to contribute to the inflammatory response in preeclampsia. This study was to investigate if vitamin D can benefit preeclampsia by inhibiting placental COX-2 expression. Placenta tissues were obtained from 40 pregnant women (23 normotensive and 17 preeclampsia). miR-26b-5p expression was assessed by quantitative PCR. Vitamin D receptor (VDR) expression and COX-2 expression were determined by immunostaining and Western blot. HTR-8/SVneo trophoblastic cells were cultured in vitro to test anti-inflammatory effects of vitamin D in placental trophoblasts treated with oxidative stress inducer CoCl. 1,25(OH)D was used as bioactive vitamin D. Our results showed that reduced VDR and miR-26b-5p expression, but increased COX-2 expression, was observed in the placentas from women with preeclampsia compared to those from normotensive pregnant women. Transient overexpression of miR-26b-5p attenuated the upregulation of COX-2 expression and prostaglandin E (PGE) production induced by CoCl in placental trophoblasts. 1,25(OH)D treatment inhibited CoCl-induced upregulation of COX-2 in placental trophoblasts. Moreover, miR-26b-5p expression were significantly upregulated in cells treated with 1,25(OH)D, but not in cells transfected with VDR siRNA. Conclusively, downregulation of VDR and miR-26b-5p expression was associated with upregulation of COX-2 expression in the placentas from women with preeclampsia. 1,25(OH)D could promote miR-26b-5p expression which in turn inhibited COX-2 expression and PGE formation in placental trophoblasts. The finding of anti-inflammatory property by vitamin D through promotion of VDR/miR-26b-5p expression provides significant evidence that downregulation of vitamin D/VDR signaling could contribute to increased inflammatory response in preeclampsia.
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http://dx.doi.org/10.1038/s41598-021-90605-9DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8160000PMC
May 2021

Two new triterpenoid glycosides from leaves of .

Nat Prod Res 2021 May 25:1-6. Epub 2021 May 25.

Xiangya School of Pharmaceutical Sciences, Central South University, Changsha, People's Republic of China.

Two dammarane glycosides () were isolated from the leaves of . The structures of new compounds were established by application of spectroscopic methods, including one-dimensional and two-dimensional NMR, HRESIMS, and chemical hydrolysis. When evaluated against seven human cancer cell lines, the two compounds exhibited selective cytotoxicity to MCF-7 cells.
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http://dx.doi.org/10.1080/14786419.2021.1931182DOI Listing
May 2021

Neuroprotective Effect of Low-Intensity Pulsed Ultrasound on the Mouse MPTP/MPP Model of Dopaminergic Neuron Injury.

Ultrasound Med Biol 2021 May 16. Epub 2021 May 16.

Pediatric Research Institute, Ministry of Education Key Laboratory of Child Development and Disorders, National Clinical Research Center for Child Health and Disorders, China International Science and Technology Cooperation Base of Child Development and Critical Disorders, Chongqing Engineering Research Center of Stem Cell Therapy, Children's Hospital of Chongqing Medical University, Chongqing, China. Electronic address:

Ultrasound mediated neuromodulation has been demonstrated to a safe treatment strategy in the field of neuroscience. In this study, low-intensity pulsed ultrasound (LIPUS) was used to treat Parkinson's disease (PD) models induced by 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP) and 1-methyl-4-phenylpyridinium (MPP) to explore the possibility of ultrasound neuroprotective effect on PD. The results demonstrated that LIPUS treatment can attenuate the central neurotoxicity of MPTP in mice, reduce the loss of tyrosine hydroxylase positive neurons in the substantia nigra pars compacta and decrease the apoptosis in the section of substantia nigra. The movement and balance dysfunctions in PD mice were improved with LIPUS treatment. In addition, we demonstrated that LIPUS can inhibit the decreased activity and increased apoptosis of dopaminergic neurons induced by MPP, restrain the accumulation of reactive oxygen species (ROS) and decrease of mitochondrial membrane potential caused by MPP. Moreover, LIPUS stimulation alone did not cause any cytotoxicity and tissue damage in our study. Taken together, the protective and regulatory effects of LIPUS on dopaminergic neurons make it possible as a new, safe and noninvasive treatment for PD.
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http://dx.doi.org/10.1016/j.ultrasmedbio.2021.03.034DOI Listing
May 2021

Multi-functional silica-based mesoporous materials for simultaneous delivery of biologically active ions and therapeutic biomolecules.

Acta Biomater 2021 May 16. Epub 2021 May 16.

Institute of Biomaterials, University of Erlangen-Nuremberg, 91058 Erlangen, Germany. Electronic address:

Mesoporous silica-based materials, especially mesoporous bioactive glasses (MBGs), are being highly considered for biomedical applications, including drug delivery and tissue engineering, not only because of their bioactivity and biocompatibility but also due to their tunable composition and potential use as drug delivery carriers owing to their controllable nanoporous structure. Numerous researches have reported that MBGs can be doped with various therapeutic ions (strontium, copper, magnesium, zinc, lithium, silver, etc.) and loaded with specific biomolecules (e.g., therapeutic drugs, antibiotics, growth factors) achieving controllable loading and release kinetics. Therefore, co-delivery of ions and biomolecules using a single MBG carrier is highly interesting as this approach provides synergistic effects toward improved therapeutic outcomes in comparison to the strategy of sole drug or ion delivery. In this review, we discuss the state-of-the-art in the field of mesoporous silica-based materials used for co-delivery of ions and therapeutic drugs with osteogenesis/cementogenesis, angiogenesis, antibacterial and anticancer properties. The analysis of the literature reveals that specially designed mesoporous nanocarriers can release multiple ions and drugs at therapeutically safe and relevant levels, achieving the desired biological effects (in vivo, in vitro) for specific biomedical applications. It is expected that this review on the ion/drug co-delivery concept using MBG carriers will shed light on the advantages of such co-delivery systems for clinical use. Areas for future research directions are identified and discussed. STATEMENT OF SIGNIFICANCE: Many studies in literature focus on the potential of single drug or ion delivery by mesoporous silica-based materials, exploiting the bioactivity, biocompatibility, tunable composition and controllable nanoporosity of these materials. Recenlty, studies have adopted the "dual-delivery" concept, by designing multi-functional mesoporous silica-based systems which are capable to deliver both biologically active ions and biomolecules (growth factors, drugs) simultaneously in order to achieve synergy of their complementary therapeutic activities. This review summarizes the state of the art in the field, with focus on osteogenesis/cementogenesis, angiogenesis, antibacterial and anticancer properties, and discusses the challenges and prospects for further progress in this area, expecting to generate broader interest in the technology for applications in disease treatment and regenerative medicine.
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http://dx.doi.org/10.1016/j.actbio.2021.05.007DOI Listing
May 2021

Expression pattern of histone lysine-specific demethylase 6B in gastric cancer.

Oncol Lett 2021 Jun 26;21(6):491. Epub 2021 Apr 26.

Department of Gastroenterology, Cixi People's Hospital, Affiliated Cixi Hospital, Wenzhou Medical University, Cixi, Zhejiang 315300, P.R. China.

Over the last few decades, predictive markers for the prognosis of gastric cancer have not been extensively investigated. The present study aimed to evaluate the expression profile of histone demethylase lysine (K)-specific demethylase 6B (KDM6B) in gastric cancer and healthy control tissues, as well as its value in prognosis prediction as a clinical marker. Within the framework of these criteria, the diagnostic role of KMD6B for gastric cancer was investigated, which may provide insights into novel treatment targets. Immunohistochemistry was applied to detect KMD6B expression in 100 gastric cancer tissues and matching para-cancerous tissues to analyze the association between KMD6B expression and clinicopathological features. Based on the follow-up data, the value of KMD6B in prognosis assessment was further explored. The role of KMD6B in gastric cancer cell proliferation, cell cycle distribution and the expression of cell cycle-associated proteins was investigated by inhibiting KMD6B activity using the specific inhibitor GSK J4. KMD6B was mostly distributed in cytoplasm and nucleus in gastric cancer tissue. The expression level was significantly higher in cancer tissues compared with that in the corresponding non-cancerous tissues. The expression of KMD6B was significantly associated with sex, lymph node and distant metastasis status and clinical stage (P<0.05). Cell proliferation was significantly decreased with the inhibition of KMD6B activity, and the cell cycle in HGC27 cells was arrested in the G/M phase after being treated with GSK J4 for 24 h. The expression of cyclin B and Cdc2 were significantly decreased, while p21 was upregulated. It was concluded that the dysregulated expression of KMD6B is associated with the malignant progression of gastric cancer and could be a potential marker for prognosis. Blocking the demethylase activity of KMD6B induced G/M arrest and inhibited the proliferation of gastric cancer cells, suggesting that KMD6B is a potential novel therapeutic target for gastric cancer.
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http://dx.doi.org/10.3892/ol.2021.12752DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8100944PMC
June 2021

PLAC1 Regulates the Occurrence of Fetal Growth Restriction by Inhibiting the Apoptosis of Trophoblast Cells.

Ann Clin Lab Sci 2021 Mar;51(2):182-189

Department of Obstetrics, Jiaxing Municipal Maternal and Child Health Care Hospital

Objective: Fetal growth restriction (FGR) refers to impaired and insufficient intrauterine growth potential caused by a variety of adverse factors and is a serious perinatal complication that leads to fetal or neonatal mortality and morbidity. FGR has numerous causes, and its pathogenesis has not been fully understood. Recently, increasing numbers of researchers have begun to focus on the placenta, the only link between the fetus and the mother. The placenta is a vital organ that plays key roles in fetal development. PLAC1 is a trophoblast-specific gene located on the X chromosome and is important for placental development. However, the biological role of PLAC1 in fetal growth restriction is not well understood. In this study, we investigated the changes in the expression of placental-specific protein 1(PLAC1) in the placentas of pregnant women with FGR and in the placentas of normal pregnancies. We also explored the regulation of PLAC1 in the growth of trophoblast cells.

Methods: Western blotting was used to detect the expression of PLAC1 in FGR and in normal placenta tissues. Cell counting kit 8 (CCK-8), wound healing, and transwell assays were used to detect the effects of PLAC1 knockdown on trophoblast cell proliferation, migration, and invasion. Western blotting was used to detect the expression of PLAC1 under hypoxic conditions, and the cell viability and apoptosis of trophoblast cells in a low oxygen concentration after overexpression of PLAC1 were detected by CCK-8 and flow cytometry assay.

Results: Compared with the placentas in the control group of normal pregnancies, the expression of PLAC1 in the placentas of the FGR group was significantly down-regulated (<0.05). Knocking down PLAC1 by siRNA significantly inhibited the proliferation, migration, and invasion of trophoblast cells. After treatment with alow oxygen concentration, the expression of PLAC1 protein was significantly reduced (<0.05). The overexpression of PLAC1 can reverse the cell viability of trophoblast cells (<0.05) and inhibit apoptosis of trophoblast cells (<0.05) in low oxygen concentration.

Conclusion: The expression of PLAC1 was reduced in fetal growth restriction and did not protect trophoblast cells from hypoxic damage, suggesting that PLAC1 may be an important regulator in the occurrence of fetal growth restriction.
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March 2021

Sarcoma with Prominent Rhabdoid Cells Mimicking Rhabdomyoblasts: Expanding the Morphologic spectrum of Sarcoma.

Int J Surg Pathol 2021 Apr 28:10668969211013891. Epub 2021 Apr 28.

12340University of Texas Health Science Center, McGovern Medical Center, Houston, TX, USA.

sarcoma (BCS) is a rare recently defined undifferentiated sarcoma that predominantly affects children and young adults. The diagnosis of this tumor is difficult due to the highly variable morphology and nonspecific immunophenotype. Emerging data suggest that patients with BCS show response to Ewing sarcoma-based treatment regimen, thus correct diagnosis is of clinical relevance. In this study, we report a case of BCS arising from the big toe of a 15-year-old male patient. The tumor had a prominent population of rhabdoid cells with bright eosinophilic cytoplasm mimicking rhabdomyosarcoma. The tumor cells were focally positive for desmin and myogenin, and negative for CD99. Next-generation sequencing showed the presence of gene fusion. BCS with prominent rhabdoid cells has not been described before. This study further expands the morphologic spectrum of BCS.
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http://dx.doi.org/10.1177/10668969211013891DOI Listing
April 2021

A Novel Rat Model with Long Range Optic Nerve Injury to Study Retinal Ganglion Cells Endogenous Regeneration.

Neuroscience 2021 06 23;465:71-84. Epub 2021 Apr 23.

The Eye Hospital, School of Ophthalmology & Optometry, Wenzhou Medical University, Wenzhou 325027, China. Electronic address:

In adult mammals, axon regeneration is limited within the lesion site after injury to the optic nerve. Changes in the microenvironment of lesion sites play an important role in retinal ganglion cells (RGCs) axon regeneration along with other intrinsic factors. In this study, the effect of the lesion site on the microenvironment and axon growth was evaluated using a refined optic nerve crush (ONC) injury model, in which the injury range was extended compared to classical injury. The number of regenerated axons labeled anterogradely with cholera toxin B fragment (CTB) was significantly increased in the long-range crush injury (LI) group compared to the ONC group at distances of 500, 1000 and 1500 µm from the initial site of the injury. These data confirmed that RGC axons can regenerate inside the lesion site. Immunofluorescence and proteomic analysis showed that the microenvironment at the lesion site was highly heterogeneous. The levels of myelin-associated inhibitors, chondroitin-sulfate proteoglycans (CSPGs) and other axon growth inhibitors decreased inside the lesion site compared to the posterior segment of the optic nerve lesion site. The expression of multiple lysosome-related enzymes, metabolic inhibitors including cholesterol esterase, cathepsin B, D, Z and arylsulfatase B (ARSB) were significantly increased inside the lesion site for the LI group compared to the normal optic nerves. Our results suggest that the model of long range optic nerve injury is more useful towards understanding the lesion microenvironment and the endogenous regeneration of RGCs. Also, we showed that myelin and neurocan (a CSPG) are differently expressed in the optic nerve between the interior and posterior lesion sites and may explain why axons cannot reach the brain through the lesion site.
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http://dx.doi.org/10.1016/j.neuroscience.2021.04.014DOI Listing
June 2021

TESOT: a teaching modality targeting the learning obstacles in global medical education.

Adv Physiol Educ 2021 Jun;45(2):333-341

Department of Physiology, School of Basic Medical Sciences, Harbin Medical University, Harbin, China.

In higher education, it is a great challenge for instructors to teach international medical students (IMSs) efficiently. These students usually have different learning obstacles and learning style preferences from domestic students. Thus it is necessary to use teaching modalities targeting the specific characteristics of IMSs. Accordingly, we have developed a teaching modality composed of classical eacher-centered approach (TCA), nriched with components of tudent-centered approach (SCA) and nline interactions argeting the learning characteristics of IMSs, which we defined as TESOT (an acronym made of the underlined words' initials). Aside from the online interactions that provide both answers to questions raised by students and guidance throughout a course, this modality contains additional in-classroom components (i.e., pre-lecture quiz, student-led summary, and post-lecture quiz). The effectiveness of this modality was tested in the nervous system module of the Physiology course for IMSs. The final exam scores in the nervous system module in the year taught with TESOT were higher than those earned by students taught with a classical TCA modality in preceding 2 yr. The improvement of teaching effectiveness is attributable to increasing communication, bridging course contexts, and meeting diverse learning style preferences. These results indicate that TESOT as an effective teaching modality is useful for enhancing efficiency of teaching IMSs.
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http://dx.doi.org/10.1152/advan.00191.2020DOI Listing
June 2021

A facile evanescent-field imaging approach for monitoring colloidal gel evolution near a surface.

Soft Matter 2021 Apr;17(15):4006-4010

Department of Chemistry, The Chinese University of Hong Kong, N.T., Shatin, Hong Kong, China.

A facile evanescent-field imaging approach is developed to probe the aggregation behavior of near-wall colloids/clusters during colloidal gel evolution. Total internal reflection microscope (TIRM) images are directly utilized to access the structural relaxation time via density-fluctuation theory. The behaviors of cluster-cluster aggregation and physical aging of the colloidal gel networks are resolved in both time and space under fractal scaling criteria.
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http://dx.doi.org/10.1039/d1sm00331cDOI Listing
April 2021

Iguratimod promotes transformation of mononuclear macrophages in elderly patients with rheumatoid arthritis by nuclear factor-κB pathway.

World J Clin Cases 2021 Apr;9(10):2181-2191

Department of Rheumatism, The First Hospital of Qiqihar, Affiliated Qiqihar Hospital of Southern Medical University, Qiqihar 161005, Heilongjiang Province, China.

Background: The role of macrophages in rheumatoid arthritis (RA) and its mechanism have attracted much attention in RA pathogenesis. Macrophages accumulate in the synoviums of RA, and the proportion of M1 type pro-inflammatory macrophages is higher than that of M2 type anti-inflammatory macrophages, leading to the secretion of inflammatory molecules and the aggravation of inflammatory reaction, which has made macrophages a potential target of RA drugs. Iguratimod is a kind of cyclo-oxygenase-2 inhibitor that affects macrophage polarity. It is speculated that its anti-inflammatory and anti-rheumatic effects may be related to the regulation of macrophage M1/M2 ratio.

Aim: To investigate the effects of Iguratimod on the polarity of mononuclear macrophages in elderly patients with RA.

Methods: Elderly patients with RA and joint effusion were selected, including 10 men and 25 women, with an average age of 66.37 ± 4.42 years. Patients were treated with oral administration of 25 mg Iguratimod (Iremod, State Food and Drug Administration Approval No. H20110084) twice daily for 12 wk. Disease Activity Score 28 and Health Assessment Questionnaire score were collected according to the disease severity before and after treatment. Venous blood and joint effusion fluid were collected, mononuclear macrophages were extracted and expression of cell surface markers CD86, CD64, CD163, and CD206 was analyzed by flow cytometry. The concentration of inflammatory factors interleukin (IL)-6, IL-1β, transforming growth factor-β, and IL-4 in the joint effusion fluid was analyzed by enzyme-linked immunosorbent assay. Expression of mononuclear cells inhibitor of nuclear factor-κB (IκB) and phosphorylated IκB in peripheral blood was analyzed by western blotting.

Results: Disease Activity Score 28 score and Health Assessment Questionnaire score of patients treated with Iguratimod decreased significantly. The percentage of cell surface markers CD86 and CD64 decreased significantly, and the percentage of CD163 and CD206 increased significantly ( < 0.05). The inflammatory factors IL-6 and IL-1β decreased significantly, and transforming growth factor-β and IL-4 increased significantly. Western blot analysis showed that mononuclear cell inhibitor of nuclear factor-κB in peripheral blood was significantly increased after treatment, and its phosphorylation level was significantly decreased ( < 0.05).

Conclusion: Iguratimod can promote the transformation of mononuclear macrophages from M1 to M2 in elderly patients with RA by inhibiting the nuclear factor-κB pathway, thus improving symptoms of RA.
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http://dx.doi.org/10.12998/wjcc.v9.i10.2181DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8026846PMC
April 2021

Microrheology of thermoresponsive poly(N-isopropylacrylamide) microgel dispersions near a substrate surface.

J Colloid Interface Sci 2021 Sep 5;597:104-113. Epub 2021 Apr 5.

Department of Chemistry, The Chinese University of Hong Kong, Shatin, N.T., Hong Kong, China. Electronic address:

Hypothesis: Relative to the bulk systems, the near-wall (<500 nm) rheological responses of soft poly(N-isopropylacrylamide) (PNIPAM) microgel dispersions may exhibit distinct dependence on the frequency (ω), temperature (T), and effective volume fraction (ϕ) during the volume phase transitions. The microrheological behaviors are expected to be governed by the near-wall microstructure and its spatial heterogeneity.

Experiments: The combination of active microrheometry (multipole magnetic tweezers) and total internal reflection microscopy (TIRM) was employed to probe the structure-rheology relationships of microgel dispersions near a substrate surface. The ω, T, and ϕ-dependences of the storage modulus (G'), loss modulus (G"), and softness (J) were analyzed by power-law and Arrhenius-like scaling theories. The fluctuation of J was further analyzed to give a quantitative description of the inhomogeneity in the near-wall regions.

Findings: (1) Remarkable differences in the rheological behaviors between the bulk and near-wall cases are revealed, where the latter shows a segmented overlap behavior in ϕ; (2) Five regimes of ϕ that correspond to distinct physical states of the microgel dispersions are determined; (3) The near-wall local structures exhibit more heterogeneity in the glass and colloidal gel regimes as compared to the liquid regime.
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http://dx.doi.org/10.1016/j.jcis.2021.03.181DOI Listing
September 2021

MRI T1 Contrast-Enhanced Signal Intensity Is a Prognostic Indicator of Imatinib Therapy in Desmoid-Type Fibromatosis.

Front Oncol 2021 15;11:615986. Epub 2021 Mar 15.

Department Radiology, Peking University Cancer Hospital, Beijing, China.

Objective: To investigate the efficiency of pre-therapy magnetic resonance imaging (MRI) features in predicting the prognosis of desmoid-type fibromatosis patients treated with imatinib.

Materials And Methods: A total of 38 desmoid-type fibromatosis patients treated with imatinib were collected in this retrospective study. The high signal intensity on pre-therapy MRI was evaluated on axial T2 and T1 contrast-enhanced sequences with fat suppression. Cox regression and Kaplan-Meier analyses explored the correlation between clinical or radiographic characteristics and progression-free survival (PFS).

Results: Hyperintense T1 contrast enhancement (CE) proportion (≥ 75%) was identified as an independent predictor for PFS. Patients with hyperintense T1 CE proportion <75% demonstrated no progression, while patients with hyperintense T1 CE proportion ≥75% demonstrated a progression rate of 78.4%.

Conclusion: Hyperintense T1 CE proportion in the tumor is a potential predictor of disease progression in patients with desmoid-type fibromatosis treated with imatinib. Hyperintense T1 CE proportion <75% indicates progression-free during treatment.
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http://dx.doi.org/10.3389/fonc.2021.615986DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8040797PMC
March 2021

Degradation and Transformation of Lignin by a Fungus Strain F-1.

Iran J Biotechnol 2020 Jul 1;18(3):e2461. Epub 2020 Jul 1.

School of Food and Biological Engineering, Hefei University of Technology, Hefei, China.

Background: Lignin is the largest natural aromatic polymer in nature and is also a unique aromatic-based biopolymer, accounting for nearly 30% of the earth's organic carbon. Generally, lignin is regarded as waste and is mainly used as a low- value fuel that is burned to generate heat and energy to solve the problem of biomass waste; for this obstacle of lignin, highly efficient biodegradation plays a critical role in developing an environmentally friendly technique for lignin biotransformation.

Objectives: This study intends to isolate and purify several microbial strains from nature. It also explores how their lignin degradation is able to enhance the biodegradation and recycling of biomass and the reclamation of lignin in wastewater from pulp and paper mills.

Materials And Methods: Lignin-degrading microbial strains were isolated from soil using medium containing sodium lignosulphonate as the sole carbon source. They were then screened by aniline blue and guaiacol plate, and then the best strain was chosen and identified. The conventional one-factor method was used to optimize various parameters that affect lignin's degradation ability.

Results: The strain possessing the highest lignin biodegradation ability was identified and denominated as F-1. After optimization, the maximum degradation rate of lignin, 44.6% within 3 days, was obtained at pH 7.0, 30 ℃, 2.5 g·L ammonium sulfate, 2 g·L lignin and 0.5 g·L glucose. The results show the LiP and Lac secreted from F-1 played the main role in the degradation of lignin.

Conclusion: One microbial strain, F-1, was successfully isolated with a lignin-degrading ability that can cut the lignin into fragments. This provides a promising candidate for the transformation and utilization of crop waste biomass for various industrial purposes.
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http://dx.doi.org/10.30498/IJB.2020.155690.2461DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8035421PMC
July 2020

Increased circulating erythrocyte-derived microparticles in patients with acute coronary syndromes.

Biomark Med 2021 Apr 9. Epub 2021 Apr 9.

Department of Cardiology, The Key Laboratory of Cardiovascular Remodeling & Function Research, Chinese Ministry of Education, Chinese National Health Commission & Chinese Academy of Medical Sciences, The State & Shandong Province Joint Key Laboratory of Translational Cardiovascular Medicine, Qilu Hospital, Cheeloo College of Medicine, Shandong University, Jinan, Shandong, China.

This study is to explore the predictive value of erythrocyte-derived microparticles (ErMPs) in patients with acute coronary syndrome (ACS). Total 305 subjects were enrolled and divided into the control group and ACS group. Flow cytometry was used to detect the ErMPs. The Gensini score was calculated based on the results of the coronary angiography. Compared with that in the control group, the ErMPs concentration in the ACS group increased significantly and the concentration of ErMPs was correlated with the ACS risk. The concentration of ErMPs and the percentage of ErMPs were positively correlated with the Gensini score. ErMPs may be a new biomarker for predicting the ACS risk and the coronary artery disease severity.
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http://dx.doi.org/10.2217/bmm-2021-0141DOI Listing
April 2021

Tumor suppressor role of hsa_circ_0035445 in gastric cancer.

J Clin Lab Anal 2021 Jun 8;35(6):e23727. Epub 2021 Apr 8.

The Affiliated People's Hospital, Ningbo University, Ningbo, China.

Circular RNAs (circRNAs) are closely related to the occurrence and development of cancers. However, the roles of circRNAs in gastric cancer are largely unknown. Total 104 pairs of gastric cancer tissues and non-cancer tissues, fasting plasma of 42 healthy people and 42 gastric cancer patients' one day before operation and 10 days after operation were collected. Quantitative reverse transcription-polymerase chain reaction was used to detect the expression level of hsa_circ_0035445. The receiver operating characteristic (ROC) curve and the area under the ROC curve (AUC) were used to analyze its diagnostic value. Small interfering RNA and overexpression vector were used to downregulate and upregulate the expression of hsa_circ_0035445, respectively. Cell Counting Kit-8 and colony formation assays were used to detect the proliferation ability. Trans-well assay and scratch assay were used to detect the migration ability. Finally, flow cytometry was used to detect the changes of cell cycle distribution and apoptosis. Hsa_circ_0035445 was lowly expressed in gastric cancer tissues, plasma of gastric cancer patients, and gastric cancer cells. The expression level of hsa_circ_0035445 in gastric cancer tissues was relationship with tumor size and distant metastasis. The AUC of hsa_circ_0035445 in tissues and plasma was 0.68 and 0.86, respectively. Upregulation of hsa_circ_0035445 suppressed the proliferation and migration, promoted apoptosis, and blocked cells at G0/G1 phase. Downregulation of hsa_circ_0035445 promoted the proliferation and migration, suppressed apoptosis, and blocked cells at S phase. In conclusion, hsa_circ_0035445 may become a new target for the treatment of gastric cancer.
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http://dx.doi.org/10.1002/jcla.23727DOI Listing
June 2021

Probiotic sp. Strains Inhibit Growth, Adhesion, Biofilm Formation, and Gene Expression of Bacterial Vaginosis-Inducing .

Microorganisms 2021 Mar 31;9(4). Epub 2021 Mar 31.

College of Pharmacy, Shanghai Jiaotong University, Shanghai 200240, China.

contributes significantly to bacterial vaginosis, which causes an ecological imbalance in vaginal microbiota and presents with the depletion of sp. supplementation was reported to be an approach to treat bacterial vaginosis. We investigated the applicability of three sp. strains ( DM8909, ATCC14917, and ZX27) based on their probiotic abilities in vitro. The three candidate sp. strains for bacterial vaginosis therapy showed distinct properties in auto-aggregation ability, hydrophobicity, adhesion to cervical epithelial cells, and survivability in 0.01% hydrogen peroxide. ZX27 showed a higher yield in producing short-chain fatty acids and lactic acid among the three candidate strains, and all three sp. strains inhibited the growth and adhesion of . Furthermore, we discovered that the culture supernatant of sp. exhibited anti-biofilm activity against . In particular, the ZX27 supernatant treatment decreased the expression of genes related to virulence factors, adhesion, biofilm formation, metabolism, and antimicrobial resistance in biofilm-forming cells and suspended cells. Moreover, sp. decreased the upregulated expression of interleukin-8 in HeLa cells induced by or hydrogen peroxide. These results demonstrate the efficacy of sp. application for treating bacterial vaginosis by limiting the growth, adhesion, biofilm formation, and virulence properties of .
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http://dx.doi.org/10.3390/microorganisms9040728DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8065998PMC
March 2021

Prognosis of severe lymphopenia after postoperative radiotherapy in non-small cell lung cancer: Results of a long-term follow up study.

Clin Transl Radiat Oncol 2021 May 12;28:54-61. Epub 2021 Mar 12.

Department of Radiation Oncology, The University of Texas MD Anderson Cancer Center, Houston, TX 77030, USA.

Purpose: To investigate the incidence and prognosis of severe radiation-induced lymphopenia (sRIL) after postoperative radiotherapy (PORT) for resected NSCLC.

Patients And Methods: Between 1998 and 2017, 170 patients treated with PORT for NSCLC were retrospectively reviewed. Lymphopenia was divided into tertiles with severe lymphopenia defined as absolute lymphocyte counts (ALC) < 0.37 × 10/ul.

Results: sRIL was observed in 32.3% of patients. Multivariable logistic regression analysis indicated sRIL was associated with planning target volume radiation fraction numbers (OR 1.09,  = 0.005) and total lung mean dose (OR 1.12,  = 0.006). With a median follow-up time of 12.2 years, the median progression-free survival and overall survival were 14.8 months and 28.4 months respectively in patients with sRIL, vs. 21.7 months ( = 0.008) and 48.3 months ( = 0.01) respectively in patients without sRIL. Multivariable analyses indicated sRIL significantly decreased OS (HR 1.95,  < 0.01). Since PORT for stage I-II NSCLC was done largely for positive margins, which may confound the contribution of severe RIL, we analyzed stage III separately and found that sRIL also significantly decreased OS (HR 1.88,  = 0.004) in multivariable analysis.

Conclusion: For this long-term outcome study, severe RIL correlated with total lung mean dose and radiation fractionation numbers, and was a strong prognostic factor for poor survival in PORT patients, particularly in patients with stage III NSCLC, highlighting the importance of an intact immune system for post-radiation immunologic disease surveillance.
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http://dx.doi.org/10.1016/j.ctro.2021.02.011DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7985216PMC
May 2021

TULP2, a New RNA-Binding Protein, Is Required for Mouse Spermatid Differentiation and Male Fertility.

Front Cell Dev Biol 2021 18;9:623738. Epub 2021 Feb 18.

State Key Laboratory of Reproductive Medicine, Department of Histology and Embryology, Nanjing Medical University, Nanjing, China.

Spermatogenesis requires a large number of proteins to be properly expressed at certain stages, during which post-transcriptional regulation plays an important role. RNA-binding proteins (RBPs) are key players in post-transcriptional regulation, but only a few RBPs have been recognized and preliminary explored their function in spermatogenesis at present. Here we identified a new RBP tubby-like protein 2 (TULP2) and found three potential deleterious missense mutations of gene in dyszoospermia patients. Therefore, we explored the function and mechanism of TULP2 in male reproduction. TULP2 was specifically expressed in the testis and localized to spermatids. Studies on knockout mice demonstrated that the loss of TULP2 led to male sterility; on the one hand, increases in elongated spermatid apoptosis and restricted spermatid release resulted in a decreased sperm count; on the other hand, the abnormal differentiation of spermatids induced defective sperm tail structures and reduced ATP contents, influencing sperm motility. Transcriptome sequencing of mouse testis revealed the potential target molecular network of TULP2, which played its role in spermatogenesis by regulating specific transcripts related to the cytoskeleton, apoptosis, RNA metabolism and biosynthesis, and energy metabolism. We also explored the potential regulator of TULP2 protein function by using immunoprecipitation and mass spectrometry analysis, indicating that TUPL2 might be recognized by CCT8 and correctly folded by the CCT complex to play a role in spermiogenesis. Our results demonstrated the important role of TULP2 in spermatid differentiation and male fertility, which could provide an effective target for the clinical diagnosis and treatment of patients with oligo-astheno-teratozoospermia, and enrich the biological theory of the role of RBPs in male reproduction.
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http://dx.doi.org/10.3389/fcell.2021.623738DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7982829PMC
February 2021

Pathogenic variants of PROC gene caused type II activity deficiency in a Chinese family: A case report.

Medicine (Baltimore) 2021 Mar;100(12):e25160

Department of Neurology, The First Hospital, Jilin University, Changchun, China.

Rationale: Hereditary Protein C (PC) deficiency is a rare genetic disorder caused by PROC gene mutation. In this article, we report a case of PC deficiency in a Chinese family due to a novel PROC gene mutation.

Study Subject: The proband presented with recurrent cerebral infarction over the course of the previous 3 years. He was admitted to the hospital due to signs of mental retardation.

Diagnoses: Physical examination, laboratory tests, and magnetic resonance imaging demonstrated that the proband had a manifestation of PC deficiency that included acute cerebral infarction. DNA sequencing analysis revealed a missense variant, c.1015G > A (p.V339 M from valine to methionine) in exon 9 of the PROC gene. In addition, Sanger sequencing confirmed that the proband's son was heterozygous for the same variant. Therefore, the PROC gene mutation was transmitted in an autosomal dominant inheritance manner.

Interventions: The patient was treated with a daily dosage of Warfarin (3.5 mg) and was scheduled to undergo regular blood coagulation tests.

Outcomes: At the 3-month follow-up appointment, the patient showed improvements in his overall health condition.

Lessons: We identified a novel missense mutation in the PROC gene in a Chinese family which caused a decrease in the PC antigen level and recurrent cerebral infarction.
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http://dx.doi.org/10.1097/MD.0000000000025160DOI Listing
March 2021

Phenotypic and biochemical characteristics and molecular basis in 36 Chinese patients with androgen receptor variants.

Orphanet J Rare Dis 2021 03 9;16(1):122. Epub 2021 Mar 9.

Department of Endocrinology, Shanghai Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, 200011, China.

Background: Androgen insensitive syndrome (AIS) is a rare genetic disease resulting from androgen receptor (AR) mutations and one of the causes of 46, XY disorder of sexual development (DSD). This study aimed to describe the clinical features and molecular defects of 36 Chinese patients with AR variants and investigate the functional alterations of novel variants in vitro.

Material And Methods: Subjects with AR variants were identified from 150 Chinese 46, XY DSD patients using targeted next-generation sequencing. In-silico and functional assays were performed to evaluate the transcriptional activity and nuclear localization of novel AR variants.

Results: Eight novel and fifteen reported AR variants were identified. 30.6% (11/36) of patients harbored additional variants other than AR. Mutations in the Arg841 residue were found in 7 unrelated patients. Postpubertal serum gonadotropin levels were significantly elevated in patients with complete AIS (CAIS) compared with those in patients with partial AIS (PAIS) (P < 0.05). All the novel variants initially predicted to be uncertain significance by in-silico analyses were reclassified as likely pathogenic for defective AR transcriptional activity in vitro, except p.L295P, which was found in an atypical patient with oligogenic mutations and reclassified as likely benign. c.368_369 ins T was observed to interfere with nuclear translocation.

Conclusions: Compared with PAIS patients, postpubertal CAIS patients had higher gonadotropin levels. Arg841 was disclosed as the location of recurrent mutations in Chinese AIS patients. Functional assays are important for reclassifying the novel AR variants and re-examining the diagnosis of AIS in specific patients with oligogenic mutations, instead of in-silico analysis.
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http://dx.doi.org/10.1186/s13023-021-01765-wDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7942007PMC
March 2021

New framework for natural-artificial transport paths and hydrological connectivity analysis in an agriculture-intensive catchment.

Water Res 2021 May 7;196:117015. Epub 2021 Mar 7.

State Key Laboratory of Water Environment Simulation, School of Environment, Beijing Normal University, Beijing 100875, PR China.

The impacts of human activities on hydrological connectivity disturb the network topology of transport paths, which has gradually evolved from natural terrain features to dual natural-artificial features. In this study, a new framework is proposed to extract information from natural-artificial transport paths and related hydrological connectivity dominated by agricultural practices and ditch networks. Graph theory and connectivity indexes are integrated for the comprehensive classification and the parallel processing of potential flow transport networks and their upstream drainage areas. Based on high-resolution remote sensing data and detailed field investigations, this new framework, which combines graph theory and connectivity indexes, is applied to a typical agriculture-intensive catchment in China. The results show that artificial factors greatly influence the transport paths and the related drainage areas. With the development of ditch construction, the hydrological transport paths become shorter and more fragmented. In addition, key ditch segments are identified by connectivity indexes, and recommendations are given for future planning. This new framework offers an approach for the hydrological connectivity analysis of complex networks and provides effective strategies for agricultural development.
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http://dx.doi.org/10.1016/j.watres.2021.117015DOI Listing
May 2021

BiOSe-Based Memristor-Aided Logic.

ACS Appl Mater Interfaces 2021 Apr 16;13(13):15391-15398. Epub 2021 Mar 16.

Department of Electronic Engineering, Chang Gung University, Guishan Dist., Taoyuan 33302, Taiwan.

The implementation of two-dimensional materials into memristor architectures has recently been a new research focus by taking advantage of their atomic thickness, unique lattice, and physical and electronic properties. Among the van der Waals family, BiOSe is an emerging ternary two-dimensional layered material with ambient stability, suitable band structure, and high conductivity that exhibits high potential for use in electronic applications. In this work, we propose and experimentally demonstrate a BiOSe-based memristor-aided logic. By carefully tuning the electric field polarity of BiOSe through a Pd contact, a reconfigurable NAND gate with zero static power consumption is realized. To provide more knowledge on NAND operation, a kinetic Monte Carlo simulation is carried out. Because the NAND gate is a universal logic gate, cascading additional NAND gates can exhibit versatile logic functions. Therefore, the proposed BiOSe-based MAGIC can be a promising building block for developing next-generation in-memory logic computers with multiple functions.
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http://dx.doi.org/10.1021/acsami.1c00177DOI Listing
April 2021

Biodegradable Zwitterionic Cream Gel for Effective Prevention of Postoperative Adhesion.

Adv Funct Mater 2021 Mar 18;31(10). Epub 2020 Dec 18.

Department of Chemical Engineering and Materials Science, Wayne State University, Detroit, 48202, USA.

Postoperative peritoneal adhesions were frequent complications for almost any types of abdominal and pelvic surgery. This led to numerous medical problems and huge financial burden to the patients. Current anti-adhesion strategies focused mostly on physical barriers including films and hydrogels. However, they can only alleviate or reduce adhesions to certain level and their applying processes were far from ideal. This work reported the development of a biodegradable zwitterionic cream gel presenting a series of characters for an idea anti-adhesion material, including unique injectable yet malleable and self-supporting properties, which enabled an instant topical application, no curing, waiting or suturing, no hemostasis requirement, protein/cell resistance and biodegradability. The cream gel showed a major advancement in anti-adhesion efficacy by completely and reliably preventing a primary and a more severe recurrent adhesion in rat models.
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http://dx.doi.org/10.1002/adfm.202009431DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7942753PMC
March 2021