Publications by authors named "Hui Zhong"

382 Publications

Objective visual quality one year after toric IOL implantation for correction of moderate and high corneal astigmatism.

Int J Ophthalmol 2021 18;14(2):245-249. Epub 2021 Feb 18.

Eye Hospital of China Academy of Chinese Medical Sciences, Beijing 100040, China.

Aim: To compare the objective visual quality after implantation of a toric intraocular lens (IOL) in order to correct moderate or high corneal astigmatism at the one year postoperative follow-up.

Methods: From December 2017 to June 2018, 66 patients (90 eyes) with simple age-related cataract with regular corneal astigmatism greater than 1.5 D were enrolled in this prospective self-control study. The patients were implanted with Proming toric IOL (model: AT3BH-AT6BH). The subjects were divided into moderate astigmatism group (46 eyes, 1.5-2.5 D) and high astigmatism group (44 eyes, >2.5 D). The uncorrected distance visual acuity, residual astigmatism and axial position of IOL were observed before operation, 3, 6mo and 1y after operation. Modulation transfer function cutoff (MTF cutoff), Strehl ratio (SR), object scatter index (OSI) were observed by OQAS II to evaluate the objective visual quality of patients.

Results: There was no significant difference in UCVA, residual astigmatism, axial deviation, MTF cutoff, SR and OSI between moderate and high astigmatism group (all >0.05). After 3mo, UCVA, MTF cutoff and SR were significantly increased (all <0.05), residual astigmatism and OSI were significantly decreased (all <0.05). After 3mo, all the indexes remained stable.

Conclusion: Proming toric IOL can effectively treat age-related cataract patients with moderate-to-high regular corneal astigmatism, correcting corneal astigmatism, improving UCVA, ensuring long-term stability in the capsule, and providing patients with better visual quality.
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http://dx.doi.org/10.18240/ijo.2021.02.10DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7840366PMC
February 2021

Bioreduction performances and mechanisms of Cr(VI) by Sporosarcina saromensis W5, a novel Cr(VI)-reducing facultative anaerobic bacteria.

J Hazard Mater 2021 Feb 13;413:125411. Epub 2021 Feb 13.

School of Minerals Processing and Bioengineering, Key Laboratory of Biohydrometallurgy of Ministry of Education, Central South University, Changsha 410083, China. Electronic address:

This study reported a novel facultative anaerobic Cr(VI)-reducing bacteria (Sporosarcina saromensis W5) and investigated its Cr(VI) removal performances and removal mechanisms. The strain W5 was able to grow and reduce Cr(VI) under aerobic and anaerobic environment, and exhibited considerable Cr(VI) reduction capabilities under a wide range of pH (8.0-13.0), temperature (20-40 °C) and initial Cr(VI) concentration (50-800 mg/L). The addition of Cd severely inhibited its growth and Cr(VI) removal, while Cu and Fe significantly enhanced the removal efficiencies. The strain W5 could utilize various electron donors and mediators to accelerate Cr(VI) reduction. Aerobic Cr(VI) reduction mainly occurred in cytoplasm and the final products were soluble organo-Cr(III) complexes. Anaerobic Cr(VI) reduction was located in both cytoplasm and membrane, and the reduction products were soluble organo-Cr(III) complexes and Cr(III) precipitates. The functional groups of hydroxyl, carboxyl and phosphoryl on cell surface participated in the combination with Cr(III). Due to its facultative anaerobic property, S. saromensis W5 offers itself as a promising engineering strain for the bioremediation of Cr(VI)-contaminated areas, especially in hypoxia environments.
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http://dx.doi.org/10.1016/j.jhazmat.2021.125411DOI Listing
February 2021

Inflammation is correlated with abnormal functional connectivity in unmedicated bipolar depression: an independent component analysis study of resting-state fMRI.

Psychol Med 2021 Feb 19:1-11. Epub 2021 Feb 19.

Medical Imaging Center, First Affiliated Hospital of Jinan University, Guangzhou510630, China.

Background: Inflammation might play a role in bipolar disorder (BD), but it remains unclear the relationship between inflammation and brain structural and functional abnormalities in patients with BD. In this study, we focused on the alterations of functional connectivity (FC), peripheral pro-inflammatory cytokines and their correlations to investigate the role of inflammation in FC in BD depression.

Methods: In this study, 42 unmedicated patients with BD II depression and 62 healthy controls (HCs) were enrolled. Resting-state-functional magnetic resonance imaging was performed in all participants and independent component analysis was used. Serum levels of Interleukin-6 (IL-6) and Interleukin-8 (IL-8) were measured in all participants. Correlation between FC values and IL-6 and IL-8 levels in BD was calculated.

Results: Compared with the HCs, BD II patients showed decreased FC in the left orbitofrontal cortex (OFC) implicating the limbic network and the right precentral gyrus implicating the somatomotor network. BD II showed increased IL-6 (p = 0.039), IL-8 (p = 0.002) levels. Moreover, abnormal FC in the right precentral gyrus were inversely correlated with the IL-8 (r = -0.458, p = 0.004) levels in BD II. No significant correlation was found between FC in the left OFC and cytokines levels.

Conclusions: Our findings that serum IL-8 levels are associated with impaired FC in the right precentral gyrus in BD II patients suggest that inflammation might play a crucial role in brain functional abnormalities in BD.
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http://dx.doi.org/10.1017/S003329172100009XDOI Listing
February 2021

Rubidium chloride modulated the fecal microbiota community in mice.

BMC Microbiol 2021 Feb 15;21(1):46. Epub 2021 Feb 15.

School of Life Sciences, Central South University, Changsha, 410013, China.

Background: The microbiota plays an important role in host health. Although rubidium (Rb) has been used to study its effects on depression and cancers, the interaction between microbial commensals and Rb is still unexplored. To gain the knowledge of the relationship between Rb and microbes, 51 mice receiving RbCl-based treatment and 13 untreated mice were evaluated for their characteristics and bacterial microbiome changes.

Results: The 16S ribosomal RNA gene sequencing of fecal microbiota showed that RbCl generally maintained fecal microbial community diversity, while the shifts in fecal microbial composition were apparent after RbCl exposure. RbCl significantly enhanced the abundances of Rikenellaceae, Alistipes, Clostridium XlVa and sulfate-reducing bacteria including Deltaproteobacteria, Desulfovibrionales, Desulfovibrionaceae and Desulfovibrio, but significantly inhibited the abundances of Tenericutes, Mollicutes, Anaeroplasmatales, Anaeroplasmataceae and Anaeroplasma lineages. With regarding to the archaea, we only observed two less richness archaea Sulfolobus and Acidiplasma at the genus level.

Conclusions: Changes of fecal microbes may in part contribute to the anticancer or anti-depressant effects of RbCl. These findings further validate that the microbiome could be a target for therapeutic intervention.
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http://dx.doi.org/10.1186/s12866-021-02095-4DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7885239PMC
February 2021

Toxicity evaluation of cadmium-containing quantum dots: A review of optimizing physicochemical properties to diminish toxicity.

Colloids Surf B Biointerfaces 2021 Feb 5;200:111609. Epub 2021 Feb 5.

School of Minerals Processing and Bioengineering, Key Laboratory of Biohydrometallurgy of Ministry of Education, Central South University, Changsha, 410083, China. Electronic address:

Fluorescent quantum dots (QDs) have received extensive attention because of their excellent optical properties and wide utilization in biological and biomedical areas. Nonetheless, there have been intense concerns on the cytotoxicity assessment of cadmium-containing QDs due to free cadmium ions release and nano-size effects. This paper reviews the representative synthetic strategies for preparation of cadmium-containing QDs and their applications. Then the toxicity assessments of QDs from cell studies to animal models are discussed, which can aid in improving our understanding of the cytotoxicity of QDs, and the toxicity mechanism is proposed. Several critical physicochemical properties of QDs are discussed and suggestions are provided for optimizing QDs design in view of minimal cytotoxicity. Finally, accurate detection techniques and systematic methodologies for the toxicity assessment of QDs are expected to achieve further breakthroughs in the future, especially in-situ, real-time, and rapid quantitative analysis methods.
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http://dx.doi.org/10.1016/j.colsurfb.2021.111609DOI Listing
February 2021

Column study of enhanced Cr(Ⅵ) removal and removal mechanisms by Sporosarcina saromensis W5 assisted bio-permeable reactive barrier.

J Hazard Mater 2021 Mar 16;405:124115. Epub 2020 Oct 16.

School of Minerals Processing and Bioengineering, Key Laboratory of Biohydrometallurgy of Ministry of Education, Central South University, Changsha 410083, China; Faculty of Materials Metallurgy & Chemistry, Jiangxi University of Science & Technology, Ganzhou, Jiangxi 341000, China. Electronic address:

In this study, the performances of Sporosarcina saromensis W5 assisted bio-permeable reactive barrier, containing activated carbon (AC) or zero-valent iron (ZVI), were investigated by column experiments in removal of Cr(Ⅵ) from simulated groundwater. The enhanced Cr(Ⅵ) removal performances were observed in biotic columns. Cr(Ⅵ) was first detected in effluent on day 24 and day 85 in Bio-AC and Bio-ZVI columns, respectively whereas it breakthrough only on day 4 and day 15 in AC and ZVI columns. Additionally, Cr(Ⅵ) removal performances induced by biofilm in Bio-QZ columns were promoted with the increase of influent Cr(Ⅵ) concentrations. According to fluorescent images, activated carbon was found to be the best biofilm carrier. Fe may not be suitable for microbial colonization because biofilm depolymerization occurred on Fe surface. Moreover, high concentration of Cr(Ⅵ) would lag the evolution of biofilm. Magnetite generating was found on the Fe surface. X-ray photoelectron spectroscopy (XPS) analysis indicated that the removal mechanism of Cr(Ⅵ) in biotic columns was biotransformation of Cr(Ⅵ) to Cr(Ш) species. Our results may provide a new insight in Cr(Ⅵ) in-situ remediation from groundwater by Bio-PRB system.
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http://dx.doi.org/10.1016/j.jhazmat.2020.124115DOI Listing
March 2021

Serotonergic Facilitation of Forelimb Functional Recovery in Rats with Cervical Spinal Cord Injury.

Neurotherapeutics 2021 Jan 8. Epub 2021 Jan 8.

Department of Integrative Biology and Physiology, University of California, Los Angeles, 610 Charles E. Young Drive, Los Angeles, CA, 90095-1527, USA.

Serotonergic agents can improve the recovery of motor ability after a spinal cord injury. Herein, we compare the effects of buspirone, a 5-HT receptor partial agonist, to fluoxetine, a selective serotonin reuptake inhibitor, on forelimb motor function recovery after a C4 bilateral dorsal funiculi crush in adult female rats. After injury, single pellet reaching performance and forelimb muscle activity decreased in all rats. From 1 to 6 weeks after injury, rats were tested on these tasks with and without buspirone (1-2 mg/kg) or fluoxetine (1-5 mg/kg). Reaching and grasping success rates of buspirone-treated rats improved rapidly within 2 weeks after injury and plateaued over the next 4 weeks of testing. Electromyography (EMG) from selected muscles in the dominant forelimb showed that buspirone-treated animals used new reaching strategies to achieve success after the injury. However, forelimb performance dramatically decreased within 2 weeks of buspirone withdrawal. In contrast, fluoxetine treatment resulted in a more progressive rate of improvement in forelimb performance over 8 weeks after injury. Neither buspirone nor fluoxetine significantly improved quadrupedal locomotion on the horizontal ladder test. The improved accuracy of reaching and grasping, patterns of muscle activity, and increased excitability of spinal motor-evoked potentials after buspirone administration reflect extensive reorganization of connectivity within and between supraspinal and spinal sensory-motor netxcopy works. Thus, both serotonergic drugs, buspirone and fluoxetine, neuromodulated these networks to physiological states that enabled markedly improved forelimb function after cervical spinal cord injury.
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http://dx.doi.org/10.1007/s13311-020-00974-8DOI Listing
January 2021

Preparation of a new β-cyclodextrin-bonded chiral stationary phase with thiocarbamated benzamide spacer for HPLC.

Anal Sci 2020 Dec 18. Epub 2020 Dec 18.

College of Chemistry, Nanchang University.

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http://dx.doi.org/10.2116/analsci.20P403DOI Listing
December 2020

Simultaneous enantiomeric determination of multiple triazole fungicides in fruits and vegetables by chiral liquid chromatography/tandem mass spectrometry on a bridged bis(β-cyclodextrin)-bonded chiral stationary phase.

Food Chem 2021 May 10;345:128842. Epub 2020 Dec 10.

College of Chemistry, Nanchang University, Nanchang 330031, China. Electronic address:

A LC-MS/MS method for simultaneous determination of twelve triazole enantiomers (hexaconazole, tebuconazole, triticonazole, flutriafol, diniconazole, paclobutrazol) in six fruits and vegetables was established based on a stable and self-made bridged bis(β-cyclodextrin)-bonded chiral stationary phase. Simultaneous enantio-separation of multiple analytes was achieved with resolution ca. 1.67-2.14. Magnetically assisted QuECHERS was used to simplify and optimize sample pre-treatment. The new method was validated (accuracy, precision, matrix effect, etc.). Good linearity (0.5-20 μg/L, R > 0.99) and high recoveries (76.1-103.4%) based on intra- and inter-day relative standard deviation (RSDs) (2.6-11.9%), were obtained. Furthermore, a total of 90 samples were analyzed using this method and enantiomeric fractions (EF) for tebuconazole in strawberry and cucumber (0.63 and 0.43, respectively) were determined as well as 0.57 for flutriafol in tomato. This high-throughput detection method supported a convenient enantiomeric monitoring for chiral pesticides in fruits and vegetables.
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http://dx.doi.org/10.1016/j.foodchem.2020.128842DOI Listing
May 2021

Ultrafast solid-liquid intercalation enabled by targeted microwave energy delivery.

Sci Adv 2020 Dec 16;6(51). Epub 2020 Dec 16.

National Synchrotron Light Source II, Brookhaven National Laboratory, Upton, NY 11973, USA.

In chemical reactions, the breaking and formation of chemical bonds usually need external energy to overcome the activation barriers. Conventional energy delivery transfers energy from heating sources via various media, hence losing efficiency and inducing side reactions. In contrast, microwave (MW) heating is known to be highly energy efficient through dipole interaction with polar media, but how exactly it transmits energy to initiate chemical reactions has been unknown. Here, we report a rigorous determination of energy delivery mechanisms underlying MW-enabled rapid hydrothermal synthesis, by monitoring the structure and temperature of all the involved components as solid-liquid intercalation reaction occurs using in situ synchrotron techniques. We reveal a hitherto unknown direct energy transmission between MW irradiation source and the targeted reactants, leading to greatly reduced energy waste, and so the ultrafast kinetics at low temperature. These findings open up new horizons for designing material synthesis reactions of high efficiency and precision.
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http://dx.doi.org/10.1126/sciadv.abd9472DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7744073PMC
December 2020

Effect of surface deformation on biocompatibility of biomedical alloys.

Mater Sci Eng C Mater Biol Appl 2021 Feb 17;119:111636. Epub 2020 Oct 17.

State Key Lab for Powder Metallurgy, Central South University, Changsha 410083, China. Electronic address:

In this study, biocompatibility of Co-29Cr-5Mo (CCM), 316L steel (316L) and Ti-6Al-4V (TC4) alloys after surface plastic deformation under the condition comparable to the human ankle activities were investigated in details. Biocompatibility of all alloys decreases after surface deformation, while it is most significantly observed in CCM alloy. The different responses of biocompatibility are related to the corresponding microstructure evolution during surface deformation: martensitic phase transformation, dislocation slipping and mechanical twinning in CCM alloy result in the extremely localized microstructure, giving rise to the obviously decreased corrosion resistance or biocompatibility; quite homogenous microstructure after surface deformation leads to the slightly decreased corrosion resistance or biocompatibility in both 316L and TC4 alloys, since the surface deformation is dominated by slipping in 316L and by both slipping and a few mechanical twinning in TC4.
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http://dx.doi.org/10.1016/j.msec.2020.111636DOI Listing
February 2021

Characteristics and outcomes of coronary artery involvement in polyarteritis nodosa.

Can J Cardiol 2020 Dec 10. Epub 2020 Dec 10.

Department of Rheumatology and Clinical Immunology, Key Laboratory of Rheumatology and Clinical Immunology, Ministry of Education, National Clinical Research Center for Dermatologic and Immunologic Diseases (NCRC-DID), Peking Union Medical College Hospital, Chinese Academy of Medical Sciences & Peking Union Medical College.1st Shuaifuyuan, Dongcheng District, Beijing, 100730, China.

Background: Coronary artery involvement is a severe but uncommon manifestation of polyarteritis nodosa (PAN), so clinicians have little knowledge of it. Our aim is to investigate the clinical characteristics, risk factors and outcomes of patients with PAN complicated with coronary artery lesions.

Methods: Data from 145 patients with PAN who were admitted to Peking Union Medical College Hospital from January 2000 to September 2019 were retrospectively collected.

Results: Nineteen (13.1%) patients had coronary artery lesions due to PAN. The age at the onset of PAN was 32.3±11.8 years. The two groups exhibited no significant differences in common risk factors for coronary arterial atherosclerosis. Affected branches of the coronary arteries were the left anterior descending branch (LAD) (15 patients), right coronary artery (RCA) (14 patients) and left circumflex branch (LCX) (9 patients). Eleven of these nineteen patients exhibited multivessel lesions. Multivariate logistic regression analysis showed that celiac artery involvement (OR 3.722, 95% CI 1.115-12.427, p=0.033) and new-onset hypertension (OR 6.668, 95% CI 1.936-22.961, p=0.003) were risk factors for coronary artery involvement in PAN patients. Stent placement was performed for 2 patients, and in-stent restenosis occurred in one patient a year later.

Conclusions: PAN with coronary artery involvement exhibits more combined involvement of arteries of other organs and more severe diseases. PAN should be considered when treating young adults with an unknown origin of coronary artery lesions. In addition to systemic immunosuppressive treatment, other measures including antiplatelet and anticoagulation therapy, should be initiated; however, determining the optimal time to perform the procedures such as intervention or surgical operations, is still challenging.
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http://dx.doi.org/10.1016/j.cjca.2020.11.011DOI Listing
December 2020

Oxalic acid modified copper tailings as an efficient adsorbent with super high capacities for the removal of Pb.

Chemosphere 2021 Jan 15;263:127833. Epub 2020 Aug 15.

: Faculty of Materials Metallurgy & Chemistry, Jiangxi University of Science & Technology, Ganzhou, Jiangxi, 341000, China.

Comprehensive utilization of tailings is not only conducive to ensuring the sustainable use of resources but also can reduce the related environmental pollution. In the present work, a new utilization way of copper tailings was proposed and a novel composite (OMT-6) was prepared by modification of tailings with oxalic acid. The composite had super high Pb adsorption capacity with the maximal Pb removal capacity of 862.07 mg/g. Its Pb removal behaviours followed pseudo-second-order kinetic equation and Langmuir model, suggesting that Pb removal depended on monolayer adsorption. The surface of OMT-6 was rough and a lot of nanospheres were loaded on its surface. The composite had mesoporous structure and a larger specific surface area compared with tailings, the above characteristics of which facilitated Pb removal. The major crystal structures of OMT-6 were transformed to CaCO•HO and FeCO•2HO after oxalic acid modification and Pb could be removed by the ions exchange between Ca, Fe and Pb. Pb removal mechanisms of OMT-6 involved ion exchange, surface complexation and electrostatic attraction interaction, among which ion exchange played a key role. These results indicated that the prepared OMT-6 composite from copper tailings was an ideal material for Pb removal from aqueous solution.
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http://dx.doi.org/10.1016/j.chemosphere.2020.127833DOI Listing
January 2021

Profiles of antibiotic resistome and microbial community in groundwater of CKDu prevalence zones in Sri Lanka.

J Hazard Mater 2021 Feb 6;403:123816. Epub 2020 Sep 6.

National Centre for Water Quality Research, National Institute of Fundamental Studies, Hantana Road, Kandy, 20000, Sri Lanka.

The chronic kidney disease of unknown etiology (CKDu) prevalent in certain regions of Sri Lanka poses a serious threat to human health. Previous epidemiological studies focused on the search of causative agents for CKDu etiology from the viewpoint of groundwater composition, but how CKDu prevalence affected the groundwater microbial composition, especially the antibiotic resistome, has never been illuminated. This study investigated the response of microbial community and antibiotic resistome to CKDu prevalence in the groundwater through the high throughput sequencing and qPCR (HT-qPCR), respectively. Results showed that CKDu prevalence significantly influenced the distribution of antibiotic resistome and microbial community composition. The mexF dominated in all the groundwater samples and could be considered as an intrinsic ARG, and the β-lactamase cphA was specially enriched and closely associated with the antibiotics used for CKDu patients. The Acinetobacter was a potential human pathogen common in the groundwater of CKDu affected regions, while CKDu prevalence specially enriched the Aeromonas. Statistical analysis indicated that CKDu prevalence impacted antibiotic resistome through the microbial community as a whole, and MGEs contributed to the occurrence of mexF, while the enrichment of cphA could be attributed to the increase of Aeromonas.
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http://dx.doi.org/10.1016/j.jhazmat.2020.123816DOI Listing
February 2021

Restoration of arm and hand functions via noninvasive cervical cord neuromodulation after traumatic brain injury: a case study.

Brain Inj 2020 12 2;34(13-14):1771-1780. Epub 2020 Dec 2.

Department of Biomedical Engineering, The Hong Kong Polytechnic University , Hung Hom, Hong Kong.

: To investigate the effects of transcutaneous electrical stimulation (tES) on upper limb functional rehabilitation in a patient with traumatic brain injury (TBI), and to identify the optimum stimulation parameters of tES. : A preliminary case study. : Two successive interventions: Phase I-voluntary physical training (vPT) and Phase II - tES along with vPT (tES+vPT). tES was delivered at C3 and C6 cervical regions. Clinical assessments presented the variation of muscle tone and motor functions, before and after each training phase, and evaluated at 1-month follow up after the last intervention. : Our results indicate that vPT alone contributed to a release of muscle spasticity of both arms of the patient with no significant improvement of hand function, while tES+vPT further reduced the spasticity of the left arm, and improved the voluntary motor function of both arms. The grip forces were also increased after the tES+vPT treatment. We found that 1 ms biphasic tES at 30 Hz produced optimum motor outputs. : The study demonstrates, for the first time, the potential benefits of cervical tES in regard to improving upper limb motor functions in a patient with chronic TBI.
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http://dx.doi.org/10.1080/02699052.2020.1850864DOI Listing
December 2020

Efficient removal of diethyl dithiocarbamate with EDTA functionalized electrolytic manganese residue and mechanism exploration.

J Hazard Mater 2020 Nov 19:124582. Epub 2020 Nov 19.

Faculty of Materials Metallurgy & Chemistry, Jiangxi University of Science & Technology, Ganzhou, Jiangxi 341000, China.

The recycling of solid wastes is obligable as it can reduce the environmental pollution and prevent the diffusion of secondary pollution. In this study, a novel cheap adsorbent was prepared by modifying electrolytic manganese residue (EMR) with EDTA. The maximum adsorption capacity of adsorbents for diethyl dithiocarbamate (DDTC) was 133.46 mg/g under initial pH of 7.32 at room temperature. Adsorption kinetics study revealed the DDTC adsorption on EDTA-EMR is mainly controlled by chemisorption and isotherm studies implied the adsorption is a monolayer process. Mechanism exploration found that the DDTC molecules could enter into the holes of EDTA-EMR, and the transition metal-based sorption sites were crucial for the target molecule immobilization and chelation. High pH value (> 10) was found to have inhibited the adsorption capacity of adsorbent, which should be due to the fact that the decreasing of functional groups on adsorbents surface and the competition between DDTC and OH. The ionic strength has negligible effect on the adsorption and the as-synthesized adsorbents showed excellent performance after five cycles. The overall results reveal that EDTA-EMR is a promising adsorbent ascribed by its low cost, good recyclability and excellent adsorption capacity.
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http://dx.doi.org/10.1016/j.jhazmat.2020.124582DOI Listing
November 2020

HDL-scavenger receptor B type 1 facilitates SARS-CoV-2 entry.

Nat Metab 2020 12 26;2(12):1391-1400. Epub 2020 Nov 26.

Beijing Institute of Biotechnology, Academy of Military Medical Sciences (AMMS), Beijing, China.

Responsible for the ongoing coronavirus disease 19 (COVID-19) pandemic, severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infects host cells through binding of the viral spike protein (SARS-2-S) to the cell-surface receptor angiotensin-converting enzyme 2 (ACE2). Here we show that the high-density lipoprotein (HDL) scavenger receptor B type 1 (SR-B1) facilitates ACE2-dependent entry of SARS-CoV-2. We find that the S1 subunit of SARS-2-S binds to cholesterol and possibly to HDL components to enhance viral uptake in vitro. SR-B1 expression facilitates SARS-CoV-2 entry into ACE2-expressing cells by augmenting virus attachment. Blockade of the cholesterol-binding site on SARS-2-S1 with a monoclonal antibody, or treatment of cultured cells with pharmacological SR-B1 antagonists, inhibits HDL-enhanced SARS-CoV-2 infection. We further show that SR-B1 is coexpressed with ACE2 in human pulmonary tissue and in several extrapulmonary tissues. Our findings reveal that SR-B1 acts as a host factor that promotes SARS-CoV-2 entry and may help explain viral tropism, identify a possible molecular connection between COVID-19 and lipoprotein metabolism, and highlight SR-B1 as a potential therapeutic target to interfere with SARS-CoV-2 infection.
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http://dx.doi.org/10.1038/s42255-020-00324-0DOI Listing
December 2020

Almost 100 % Peroxymonosulfate Conversion to Singlet Oxygen on Single-Atom CoN Sites.

Angew Chem Int Ed Engl 2021 Feb 8;60(9):4588-4593. Epub 2021 Jan 8.

MOE Key Laboratory of Pollution Processes and Environmental Criteria, Tianjin Key Laboratory of Environmental Remediation and Pollution Control, College of Environmental Science and Engineering, Nankai University, Tianjin, 300350, P. R. China.

Single-atom CoN active sites have demonstrated excellent efficiency in peroxymonosulfate activation. However, the identification of CoN active sites and the detailed singlet oxygen generation mechanism in peroxymonosulfate activation remains ambiguous. We demonstrate a strategy to regulate the generation of reactive oxygen species by atomically dispersed cobalt anchored on nitrogen-doped carbon. As indicated by experiment and DFT calculations, CoN was the active site and singlet oxygen was the predominant reactive oxygen species with a proportion of 98.89 %. Spontaneous dissociation of adsorbed peroxymonosulfate on the CoN active sites was energetically unfavorable because of the weakly positive Co atoms and CoN coordination, which directed PMS oxidation by a non-radical pathway and with simultaneous singlet oxygen generation. The generated singlet oxygen degraded several organic pollutants with high efficiency across a broad pH range.
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http://dx.doi.org/10.1002/anie.202014472DOI Listing
February 2021

Insulin Promotes Mitochondrial Respiration and Survival through PI3K/AKT/GSK3 Pathway in Human Embryonic Stem Cells.

Stem Cell Reports 2020 12 12;15(6):1362-1376. Epub 2020 Nov 12.

Centre of Reproduction, Development and Aging, Faculty of Health Sciences, University of Macau, Taipa, Macau SAR, China; Institute of Translational Medicine, Faculty of Health Sciences, University of Macau, Macau SAR, China. Electronic address:

Insulin is an essential growth factor for the survival and self-renewal of human embryonic stem cells (hESCs). Although it is best known as the principal hormone promoting glycolysis in somatic cells, insulin's roles in hESC energy metabolism remain unclear. In this report, we demonstrate that insulin is essential to sustain hESC mitochondrial respiration that is rapidly decreased upon insulin removal. Insulin-dependent mitochondrial respiration is stem cell specific, and mainly relies on pyruvate and glutamine, while glucose suppresses excessive oxidative phosphorylation. Pharmacologic and genetic manipulations reveal that continuous insulin signal sustains mitochondrial respiration through PI3K/AKT activation and downstream GSK3 inhibition. We further show that insulin acts through GSK3 inhibition to suppress caspase activation and rescue cell survival. This study uncovers a critical role of the AKT/GSK3 pathway in the regulation of mitochondrial respiration and cell survival, highlighting insulin as an essential factor for accurate assessment of mitochondrial respiration in hESCs.
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http://dx.doi.org/10.1016/j.stemcr.2020.10.008DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7724469PMC
December 2020

Risk and prognosis factors for systemic sclerosis with lung cancer: A single-centre case-control study in China.

Int J Clin Pract 2020 Nov 6:e13819. Epub 2020 Nov 6.

Department of Rheumatology and Clinical Immunology of Peking Union Medical College Hospital, Chinese Academy of Medical Sciences & Peking Union Medical College, Key Laboratory of Rheumatology and Clinical Immunology, Ministry of Education, Beijing, China.

Objective: To analyse clinical characteristics, risk and prognosis factors for systemic sclerosis (SSc) patients with lung cancer.

Methods: SSc patients with lung cancer admitted to Peking Union Medical College Hospital from February 1992 to December 2018 were included. Age and sex-matched controls were selected from a pool of SSc patients without lung cancer during the same period. Conditional logistic regression and Cox proportional-hazard regression were used to identify risk factors and prognosis factors. The Kaplan-Meier method was used to draw the survival curve and calculate median survival.

Results: Nineteen SSc patients with lung cancer and 76 controls were included. The mean age at lung cancer diagnosis was 54.4 ± 10.2 years. In all 19 cases the lung cancer had been diagnosed after SSc and the median interval between SSc onset and lung cancer onset was 10.5 years (range 2.0-36.2 years). Among SSc patients with lung cancer, the median follow-up time and median survival were 2.6 years and 1.4 years, respectively. In the sex and age-matched conditional logistic multivariable regression analysis, family history of malignancy (OR 4.930, 95%CI 1.926-12.619, P = .001), ILD (OR 7.701, 95%CI 1.009-58.767, P = .049) were independent risk factors for lung cancer among SSc patients, and considering sex and age of SSc onset, SSc patients with more advanced staging of lung cancer (HR 3.190, 95%CI 1.127-6.126, P = .06) had poorer prognosis.

Conclusion: Lung cancer is not uncommon in SSc patients, especially those with family histories of malignancy or ILD. Early detection of lung cancer is of vital importance for better prognosis.
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http://dx.doi.org/10.1111/ijcp.13819DOI Listing
November 2020

Hemolysis inhibits humoral B-cell responses and modulates alloimmunization risk in patients with sickle cell disease.

Blood 2021 Jan;137(2):269-280

Laboratory of Immune Regulation, and.

Red blood cell alloimmunization remains a barrier for safe and effective transfusions in sickle cell disease (SCD), but the associated risk factors remain largely unknown. Intravascular hemolysis, a hallmark of SCD, results in the release of heme with potent immunomodulatory activity, although its effect on SCD humoral response, specifically alloimmunization, remains unclear. Here, we found that cell-free heme suppresses human B-cell plasmablast and plasma cell differentiation by inhibiting the DOCK8/STAT3 signaling pathway, which is critical for B-cell activation, as well as by upregulating heme oxygenase 1 (HO-1) through its enzymatic byproducts, carbon monoxide and biliverdin. Whereas nonalloimmunized SCD B cells were inhibited by exogenous heme, B cells from the alloimmunized group were nonresponsive to heme inhibition and readily differentiated into plasma cells. Consistent with a differential B-cell response to hemolysis, we found elevated B-cell basal levels of DOCK8 and higher HO-1-mediated inhibition of activated B cells in nonalloimmunized compared with alloimmunized SCD patients. To overcome the alloimmunized B-cell heme insensitivity, we screened several heme-binding molecules and identified quinine as a potent inhibitor of B-cell activity, reversing the resistance to heme suppression in alloimmunized patients. B-cell inhibition by quinine occurred only in the presence of heme and through HO-1 induction. Altogether, these data suggest that hemolysis can dampen the humoral B-cell response and that B-cell heme responsiveness maybe a determinant of alloimmunization risk in SCD. By restoring B-cell heme sensitivity, quinine may have therapeutic potential to prevent and inhibit alloimmunization in SCD patients.
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http://dx.doi.org/10.1182/blood.2020008511DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7820872PMC
January 2021

Study on the oxidative stress and transcriptional level in Cr(VI) and Hg(II) reducing strain Acinetobacter indicus yy-1 isolated from chromium-contaminated soil.

Chemosphere 2021 Apr 26;269:128741. Epub 2020 Oct 26.

School of Minerals Processing and Bioengineering, Key Laboratory of Biohydrometallurgy of Ministry of Education, Central South University, Changsha, 410083, China. Electronic address:

The bioreduction of Cr(VI) and Hg(II) has become a hot topic in the field of heavy metals bioremediation. However, the mechanism of antioxidant stress in Cr(VI) and Hg(II) reducing bacteria is still not clear. In this work, a novel Cr(VI) and Hg(II) reducing strain Acinetobacter indicus yy-1, was isolated from chromium landfill at a chromate factory, which was used to investigate the mechanism of antioxidant stress during the Cr(VI) and Hg(II) reduction process. The results demonstrated that the removal of Cr(VI) and Hg(II) by A. indicus yy-1 from solution was through reduction rather than biosorption. The reduction rates of Cr(VI) and Hg(II) by resting cells reached 59.71% and 31.73% at 24 h with initial concentration of 10 mg L, respectively. X-ray photoelectron spectroscopy (XPS) analysis further showed that Cr(III) and Hg(0) were mainly the Cr(VI)- and Hg(II)-reduced productions, respectively. Results of physiological assays showed Hg(II) was more toxic to A. indicus yy-1 than Cr(VI), and the activities of antioxidant enzymes (SOD and CAT) were significantly increased in A. indicus yy-1 for relieving the oxidative stress. The transcriptional level of genes related to Cr(VI) and Hg(II) reductases and antioxidant enzymes were up-regulated, indicating that the reductases have participated in the reduction of Cr(VI) and Hg(II), and SOD and CAT served as the vital antioxidant enzymes for defending the oxidative stress. This work provides a deep insight into the mechanism of antioxidant stress in Cr(VI) and Hg(II) reducing bacteria, which helps seek the highly resistant heavy metal reducing bacteria.
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http://dx.doi.org/10.1016/j.chemosphere.2020.128741DOI Listing
April 2021

[Distribution of Antibiotic Concentration in Domestic Wastewater Treatment Facilities in Villages and Towns].

Huan Jing Ke Xue 2020 Nov;41(11):5008-5015

State Key Joint Laboratory of Environmental Simulation and Pollution Control, Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences, Beijing 100085, China.

Antibiotics discharged into the environment cause increased environmental resistance. Four types of antibiotics (quinolones, tetracyclines, macrolides, and -lactams) were selected for this study. In a comparison with the municipal wastewater plant, the concentration and removal of antibiotics in influent and effluent of domestic wastewater treatment facilities of different scales in villages and towns was investigated using high-performance liquid chromatography (HPLC) and tandem mass spectrometry (MS). The results showed that the highest amount of ofloxacin in rural wastewater treatment facilities reached 32663.5 ng·L. Due to the different situations of influent fluctuation, discharge requirements, and management between urban and rural wastewater plants, only 33% of the rural domestic wastewater facilities detected an antibiotics removal rate of more than 60%. The effective removal of some antibiotics can be achieved when the rural domestic wastewater treatment facilities maintain the standard discharge of conventional pollutants.
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http://dx.doi.org/10.13227/j.hjkx.202003143DOI Listing
November 2020

The Type III Secretion Effector IpaH4.5 Targets NLRP3 to Activate Inflammasome Signaling.

Front Cell Infect Microbiol 2020 30;10:511798. Epub 2020 Sep 30.

Department of Genetic Engineering, Beijing Institute of Biotechnology, Beijing, China.

Activation of the NLRP3 inflammasome requires the expression of NLRP3, which is strictly regulated by its capacity to directly recognize microbial-derived substances. Even though the involvement of caspase-1 activation in macrophages NLRP3 and NLRC4 has been discovered, the accurate mechanisms by which infection triggers NLRP3 activation remain inadequately understood. Here, we demonstrate that IpaH4.5, a T3SS effector, triggers inflammasome activation by regulating NLRP3 expression through the E3 ubiquitin ligase activity of IpaH4.5. First, we found that IpaH4.5 interacted with NLRP3. As a result, IpaH4.5 modulated NLRP3 protein stability and inflammasome activation. Bacteria lacking IpaH4.5 had dramatically reduced ability to induce pyroptosis. Our results identify a previously unrecognized target of IpaH4.5 in the regulation of inflammasome signaling and clarify the molecular basis for the cytosolic response to the T3SS effector.
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http://dx.doi.org/10.3389/fcimb.2020.511798DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7561375PMC
September 2020

[Ultrasound-guided reduction and percutaneous crossed pin fixation for the treatment of displaced supracondylar fracture of the humerus in children].

Zhongguo Gu Shang 2020 Oct;33(10):907-11

Jiangnan Hospital Affiliated to Zhejiang University of Traditional Chinese Medicine, Hangzhou 311201, Zhejiang, China.

Objective: To investigate the effect of ultrasound guided reduction and exploration of ulnar nerve position and percutaneous crossed pin fixation for the treatment of displaced supracondylar fracture of the humerus in children.

Methods: The clinical data of 45 patients with displaced supracondylar fracture of humerus from December 2017 to December 2018 were analyzed retrospectively, including 26 boys and 19 girls, ranging in age from 1 year and 3 months to 11 years and 4 months, with an average of 7.6 years old;44 cases of crashing injury, 1 case of falling injury;29 cases on the left side, 16 cases on the right side;12 patients classified to Gartland typeⅡand 33 patients classified to Gartlandtype Ⅲ. The operation was performed from 4 h to 7 d after injury, with an average of 2.5 d. There were no neurological and vascular injuries occurred in the children. Ultrasound was used to guide the fracture reduction of the child, and the cross-needle was fixed. In the medial needle insertion, the ulnar nerve position was detected by ultrasound to avoid damage to the ulnar nerve. The ulnar nerve state was observed during operation. The clinical function evaluation criteria of Flynn was used at the latest follow-up. The evaluation criteria was used to evaluate the functional and aesthetic characteristics of the elbow joints of the children, and to observe the complications such as ulnar nerve injury after operation.

Results: Ultrasound was used to detect the fracture from the medial side of the elbow, the lateral aspect of the elbow and the sagittal plane of the elbow. The position of the ulnar nerve could be clearly explored to avoid ulnar nerve injury when the needle was inserted inside. All children were followed up, and the duration ranged from 6 to 12 months, with an average of 9 months. None of the patients had a loss of repositioning and the fractures healed. The healing time ranged from 4 to 6 weeks, with an average of 5 weeks. At the latest follow-up, according to Flynn's evaluation criteria:compared with the healthy side, 41 patients with flexion and extension limitation were 0° to 5°, clinically evaluated as excellent;3 patients with flexion and extension limitation were 6° to 10°, clinically evaluated as good;1 patient with flexion and extension limitation was 11° to 15°, clinically evaluated as acceptable. Compared with the healthy side, 40 patients lost 0° to 5°of the angle, and 5 patients lost 6° to 10°of the angle. There were no complications such as ulnar nerve injury and cubitus varus.

Conclusion: Although ultrasound-guided treatment of displaced supracondylar fracture of the humerus in children has higher requirements for the operator at present, because of its advantages of clear development, portability, effectiveness and no impact on health, it could clearly explorethe fracture situation during the operation, guide the reduction of the fracture, and accurately show the position of the ulnar nerve, effectively improve the safety of the medial puncture, so as to minimize the complications The occurrence of the disease. Therefore, the treatment of displaced supracondylar fracture of humerus with ultrasound-guided manual reduction and percutaneous cross needle fixation is effective and worthy of further promotion.
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http://dx.doi.org/10.12200/j.issn.1003-0034.2020.10.004DOI Listing
October 2020

Plastome structure and adaptive evolution of s.l. species.

PeerJ 2020 13;8:e10051. Epub 2020 Oct 13.

Key Laboratory of National Forestry and Grassland Administration for Orchid Conservation and Utilization at College of Landscape Architecture, Fujian Agriculture and Forestry University, Fuzhou, Fujian, China.

s.l. is the most diverse group in the tribe Collabieae (Orchidaceae), which are pantropical in distribution. Illumina sequencing followed by assembly was used in this study, and the plastid genetic information of s.l. was used to investigate the adaptive evolution of this taxon. Herein, the complete plastome of five s.l. species (, , , , and ) were determined, and the two other published plastome sequences of s.l. were added for comparative analyses to examine the evolutionary pattern of the plastome in the alliance. The seven plastomes ranged from 150,181 bp () to 159,014 bp () in length and were all mapped as circular structures. Except for the three genes (, , and ) lost in . , the remaining six species contain identical gene orders and numbers (115 gene). Nucleotide diversity was detected across the plastomes, and we screened 14 mutational hotspot regions, including 12 non-coding regions and two gene regions. For the adaptive evolution investigation, three species showed positive selected genes compared with others, (), ( and ) and ( and ). Six genes were under site-specific positive selection in s.l., namely, , , , , , and , most of which are involved in photosynthesis. These results, including the new plastomes, provide resources for the comparative plastome, breeding, and plastid genetic engineering of orchids and flowering plants.
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http://dx.doi.org/10.7717/peerj.10051DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7566753PMC
October 2020

Fabrication of attapulgite-based dual responsive composite hydrogel and its efficient adsorption for methyl violet.

Environ Technol 2020 Nov 8:1-13. Epub 2020 Nov 8.

Key Laboratory of Sensor Analysis of Tumor Marker, Ministry of Education, College of Chemistry and Molecular Engineering, Qingdao University of Science and Technology, Qingdao, People's Republic of China.

In this work, attapulgite (ATP)-based dual sensitive poly (N-isopropylacrylamide-co-acrylic acid) composite hydrogel, P(NIPAM-co-AA)/ATP, was prepared by free radical polymerization. The prepared composite hydrogel was characterized via methods of scanning electron microscopy (SEM), Fourier transform infrared spectroscopy (FT-IR), zeta potential analysis and Brunauer, Emmett, and Teller (BET) etc. The composite hydrogel showed pH and temperature sensitive behaviour, with lower critical solution temperature (LCST) of 35°C and highest swelling occurred at pH 8.0. The adsorption of methyl violet (MV) can be controlled by the hydrogel responsiveness, and 95.78% of MV can be removed at pH 8.0 and 35°C. The addition of a small amount of ATP (3 Wt%) can improve the swelling ratio and adsorption capacity. Kinetic analysis demonstrated that the experimental data were best fitted to the pseudo-second order model. Isotherm analysis showed that the equilibrium data followed Langmuir model with the adsorption capacity of 168.35 mg g. In addition, the composite hydrogel has high adsorption selectivity for cationic dyes, and MV-loaded hydrogel is easy to regenerate, which can be used for successive adsorption cycles. These results demonstrate that the composite hydrogel has potential application in dye wastewater treatment.
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http://dx.doi.org/10.1080/09593330.2020.1838623DOI Listing
November 2020

Efficient removal of Hg from aqueous solution by a novel composite of nano humboldtine decorated almandine (NHDA): Ion exchange, reducing-oxidation and adsorption.

J Hazard Mater 2021 Feb 28;404(Pt A):124035. Epub 2020 Sep 28.

Faculty of Materials Metallurgy & Chemistry, Jiangxi University of Science & Technology, Ganzhou, Jiangxi 341000, China.

Efficient removal of Hg from aqueous solution is key for environmental protection and human health. Herein, a novel composite of nano humboldtine decorated almandine was synthesized from almandine for the removal of Hg. Results showed that the Hg removal process followed pseudo-second-order kinetic model and Langmuir equation, and the maximum adsorption capacity was 575.17 mg/g. Furthermore, Hg removal by the composite was pH-dependent and low pH value facilitated the removal of Hg. SEM and HADDF-STEM results suggested a new rod morphology was generated and the adsorbed mercury was mainly enriched into this structure after reaction with Hg solution. The removal mechanisms of Hg by the composite was pH dependent, and included ion exchange, surface complexation, reduction and oxidation. Our results demonstrated that the composite was an ideal material for Hg removal and the transformation ways of mercury related species could be a significant but currently underestimated pathway in natural and engineered systems.
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http://dx.doi.org/10.1016/j.jhazmat.2020.124035DOI Listing
February 2021

Redundancy and multifunctionality among spinal locomotor networks.

J Neurophysiol 2020 11 23;124(5):1469-1479. Epub 2020 Sep 23.

Department of Integrative Biology and Physiology, University of California, Los Angeles, California.

c-Fos is used to identify system-wide neural activation with cellular resolution in vivo. However, c-Fos can only capture neural activation of one event. Targeted recombination in active populations (TRAP) allows the capture of two different c-Fos activation patterns in the same animal. So far, TRAP has only been used to examine brain circuits. This study uses TRAP to investigate spinal circuit activation during resting and stepping, giving novel insights of network activation during these events. The level of colabeled (c-Fos+ and TRAP+) neurons observed after performing two bouts of stepping suggests that there is a probabilistic-like phenomenon that can recruit many combinations of neural populations (synapses) when repetitively generating many step cycles. Between two 30-min bouts of stepping, each consisting of thousands of steps, only ∼20% of the neurons activated from the first bout of stepping were also activated by the second bout. We also show colabeling of interneurons that have been active during stepping and resting. The use of the FosTRAP methodology in the spinal cord provides a new tool to compare the engagement of different populations of spinal interneurons in vivo under different motor tasks or under different conditions. The results are consistent with there being an extensive amount of redundancy among spinal locomotor circuits. Using the newly developed FosTRAP mouse model, only ∼20% of neurons that were active (labeled by -linked tdTomato expression) during a first bout of 30-min stepping were also labeled for c-Fos during a second bout of stepping. This finding suggests variability of neural networks that enables selection of many combinations of neurons (synapses) when generating each step cycle.
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http://dx.doi.org/10.1152/jn.00338.2020DOI Listing
November 2020

Large-scale production, purification, and function of a tumor multi-epitope vaccine: Peptibody with bFGF/VEGFA.

Eng Life Sci 2020 Sep 29;20(9-10):422-436. Epub 2020 Jul 29.

Guangdong Province Engineering Research Center for Antibody Drug and Immunoassay Department of Biology Jinan University Guangzhou P. R. China.

In tumor tissue, basic fibroblast growth factor (bFGF) and vascular endothelial growth factor A (VEGFA) promote tumorigenesis by activating angiogenesis, but targeting single factor may produce drug resistance and compensatory angiogenesis. The Peptibody with bFGF/VEGFA was designed to simultaneously blockade these two factors. We were aiming to produce this Fc fusion protein in a large scale. The biological characterizations of Peptibody strains were identified as and the fermentation mode was optimized in the shake flasks and 10-L bioreactor. The fermentation was scaled up to 100 L, with wet cell weight (WCW) 126 g/L, production 1.41 g/L, and productivity 0.35 g/(L·h) of IPTG induction. The target protein was isolated by cation-exchange, hydrophobic and Protein A chromatography, with total recovery of 60.28% and HPLC purity of 86.71%. The host cells protein, DNA, and endotoxin residues were within the threshold. In mouse model, immunization of Peptibody vaccine could significantly suppressed the tumor growth and angiogenesis, with inhibition rate of 57.73 and 39.34%. The Peptibody vaccine could elicit high-titer anti-bFGF and anti-VEGFA antibodies, which inhibited the proliferation and migration of Lewis lung cancer cell cells by decreasing the Akt/MAPK signal pathways. Therefore, the Peptibody with bFGF/VEGFA might be used as a therapeutic tumor vaccine. The large-scale process we developed could support its industrial production and pre-clinical study in the future.
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http://dx.doi.org/10.1002/elsc.202000020DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7481771PMC
September 2020