Publications by authors named "Hui Zhang"

7,883 Publications

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Rapid Screening of Novel Dipeptidyl Peptidase-4 Inhibitory Peptides from Pea () Protein Using Peptidomics and Molecular Docking.

J Agric Food Chem 2022 Aug 11. Epub 2022 Aug 11.

National Engineering Research Center for Functional Food, Collaborative Innovation Center of Food Safety and Quality Control in Jiangsu Province, School of Food Science and Technology, Jiangnan University, 1800 Lihu Road, Wuxi, 214122, China.

Pea protein hydrolysates (PPHs) possess good hypoglycemic effects; however, their dipeptidyl peptidase-4 (DPP-4) inhibitory activity is poorly understood, and none of the DPP-4 inhibitory peptides have been identified from PPHs. This paper aims to rapidly screen these peptides from PPHs by combining peptidomics and molecular docking. In this study, 543 peptides were identified by peptidomics, and four peptides (IPYWTY, IPYWT, LPNYN, and LAFPGSS) with DPP-4 half-maximal inhibitory concentration (IC) values <100 μM were screened for the first time. Significantly, peptide IPYWTY exhibited the most potent DPP-4 inhibitory activity (IC = 11.04 μM) mainly because it formed hydrophobic interactions with the S1 pocket in DPP-4. These results indicated that combining peptidomics and molecular docking is an effective strategy for rapidly screening DPP-4 inhibitory peptides.
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http://dx.doi.org/10.1021/acs.jafc.2c03949DOI Listing
August 2022

Adrenal Gland Metastasis of Breast Invasive Mucinous Carcinoma: A Rare Case Report and Review of Literature.

Case Rep Oncol 2022 May-Aug;15(2):636-641. Epub 2022 Jun 23.

Department of Urology, Shandong University of Traditional Chinese Medicine, Jinan, China.

The adrenal gland is a frequent site for metastasis, and the majority of the metastatic lesions of the adrenal gland normally originate from lung cancer, colon cancer, renal cell carcinoma, and melanoma. However, adrenal gland metastasis from breast invasive mucinous carcinoma is extremely rare. This report described a rare case of right adrenal gland metastasis in a 48-year-old female, who was diagnosed with breast invasive mucinous carcinoma and underwent right modified radical mastectomy with axillary lymph node dissection 5 years previously. A mass located on the right adrenal gland was detected during a routine examination 2 months ago. The patient was asymptomatic and adrenal gland MRI revealed a mass in the right adrenal gland. Definitive preoperative diagnosis failed to be established. Right adrenal gland laparoscopic adrenalectomy was performed and the diagnosis of adrenal gland metastasis of breast carcinoma was confirmed by pathological and immunohistochemical examination, especially ER, PR, GATA3, and HER-2. The patient remained in good condition by the time of writing.
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http://dx.doi.org/10.1159/000525214DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9251452PMC
June 2022

Value of magnetic resonance diffusion combined with perfusion imaging techniques for diagnosing potentially malignant breast lesions.

World J Clin Cases 2022 Jun;10(18):6021-6031

Department of Radiology, the First Hospital of Hebei Medical University, Shijiazhuang 050000, Hebei Province, China.

Background: Lesions of breast imaging reporting and data system (BI-RADS) 4 at mammography vary from benign to malignant, leading to difficulties for clinicians to distinguish between them. The specificity of magnetic resonance imaging (MRI) in detecting breast is relatively low, leading to many false-positive results and high rates of re-examination or biopsy. Diffusion-weighted imaging (DWI), combined with perfusion-weighted imaging (PWI), might help to distinguish between benign and malignant BI-RADS 4 breast lesions at mammography.

Aim: To evaluate the value of DWI and PWI in diagnosing BI-RADS 4 breast lesions.

Methods: This is a retrospective study which included patients who underwent breast MRI between May 2017 and May 2019 in the hospital. The lesions were divided into benign and malignant groups according to the classification of histopathological results. The diagnostic efficacy of DWI and PWI were analyzed respectively and combinedly. The 95 lesions were divided according to histopathological diagnosis, with 46 benign and 49 malignant. The main statistical methods used included the Student t-test, the Mann-Whitney U-test, the chi-square test or Fisher's exact test.

Results: The mean apparent diffusion coefficient (ADC) values in the parenchyma and lesion area of the normal mammary gland were 1.82 ± 0.22 × 10 mm/s and 1.24 ± 0.16 × 10 mm/s, respectively ( = 0.021). The mean ADC value of the malignant group was 1.09 ± 0.23 × 10 mm/s, which was lower than that of the benign group (1.42 ± 0.68 × 10 mm/s) ( = 0.016). The volume transfer constant (Ktrans) and rate constant (Kep) values were higher in malignant lesions than in benign ones (all < 0.001), but there were no significant statistical differences regarding volume fraction (V) ( = 0.866). The sensitivity and specificity of PWI combined with DWI (91.7% and 89.3%, respectively) were higher than that of PWI or DWI alone. The accuracy of PWI combined with DWI in predicting pathological results was significantly higher than that predicted by PWI or DWI alone.

Conclusion: DWI, combined with PWI, might possibly distinguish between benign and malignant BI-RADS 4 breast lesions at mammography.
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http://dx.doi.org/10.12998/wjcc.v10.i18.6021DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9254209PMC
June 2022

A Phase I/II Study of Intrathecal Trastuzumab in HER-2 Positive Cancer with Leptomeningeal Metastases: Safety, Efficacy, and Cerebrospinal Fluid Pharmacokinetics.

Neuro Oncol 2022 Aug 10. Epub 2022 Aug 10.

Department of Neurology at The Feinberg School of Medicine at Northwestern University and The Malnati Brain Tumor Institute at the Robert H. Lurie Comprehensive Cancer Center of Northwestern University. Chicago, IL.

Background: Patients with human epidermal growth factor receptor 2-positive (HER2-positive) cancers have a high incidence of central nervous system (CNS) spread, but unfortunately systemic trastuzumab which targets the HER2 receptor has little CNS penetration. The purpose of this study was to determine the maximum tolerated dose of intrathecal trastuzumab and its efficacy in patients with HER2-positive LMD.

Methods: This multicenter study enrolled 34 LMD patients in a combined Phase I/II study in treating patients with intrathecal trastuzumab. Any HER2-positive histology was allowed in the Phase I; the Phase II was limited to HER2-positive breast cancer.

Results: Intrathecal trastuzumab was well tolerated, with one dose limiting toxicity of grade 4 (arachnoiditis) occurring at the 80 mg twice weekly dose. The recommended Phase II dose was 80 mg intrathecally twice weekly. Twenty-six patients at dose level 80mg were included in evaluation for efficacy: partial response was seen in 5 (19.2%) patients, stable disease was observed in 13 (50.0%), and 8 (30.8%) of the patients had progressive disease. Median overall survival (OS) for Phase 2 dose treated patients was 8.3 months (95% CI 5.2 to 19.6). The Phase II HER2-positive breast cancer patients median OS was 10.5 months (95% CI 5.2 to 20.9). Pharmacokinetic (PK) studies were limited in the setting of concurrent systemic trastuzumab administration, however, did show stable CSF concentrations with repeated dosing suggest that trastuzumab does not accumulate in the CSF in toxic concentrations.

Conclusion: This study suggests promise for potentially improved outcomes of HER-positive LMD patients when treated with intrathecal trastuzumab while remaining safe and well-tolerated for patients.
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http://dx.doi.org/10.1093/neuonc/noac195DOI Listing
August 2022

Thymectomy in ocular myasthenia gravis-prognosis and risk factors analysis.

Orphanet J Rare Dis 2022 Aug 9;17(1):309. Epub 2022 Aug 9.

Department of Cardiothoracic Surgery, Tianjin Medical University General Hospital, No.154, Anshan Road, Tianjin, China.

Background: Several retrospective studies have identified risk factors associated with ocular myasthenia gravis (OMG) generalization in non-surgical patients. However, the outcomes of OMG after thymectomy have not been investigated fully. This study aimed to explore the clinical predictors of post-thymectomy OMG prognosis.

Methods: We performed a retrospective review of OMG patients who underwent thymectomy at our institution from January 2012 to December 2021. Kaplan-Meier and Cox proportional hazard regression analyses were used to evaluate associations between clinical features and prognosis. The main outcome measures were OMG conversion, complete stable remission (CSR), and clinical improvement.

Results: Fifty-eight patients were identified for conversion analysis. Thirteen (22.4%) developed generalized myasthenia gravis (GMG) at a median time of 12.7 (3-37.3) months from symptom onset. Repetitive nerve stimulation (RNS)-positivity was associated with increased risk of conversion to GMG (P = 0.002). Patients with histotype B2/B3 thymoma showed a higher risk of conversion (P = 0.002) than did patients with hyperplasia and AB/B1 thymoma. Fifty-two patients fulfilled the criteria for CSR and improvement. Sixteen (30.8%) achieved CSR at a median time of 28.7 (15-54) months after thymectomy. Fifteen (28.8%) showed clinical improvement at last follow up. Patients who achieved CSR showed a younger age of onset (P = 0.022), lower percentage of acetylcholine receptor antibody-seropositivity (P = 0.029). Histologically, patients with thymic hyperplasia and stage I thymoma showed a higher chance of CSR (P = 0.010) than did patients with stage II/III thymoma. Multivariate analysis revealed that RNS-positivity (hazard ratio [HR] 6.007, P = 0.021) and histotype B2/B3 thymoma (HR 4.611, P = 0.048) were associated with OMG conversion. Thymic hyperplasia and stage I thymoma (HR 0.300, P = 0.026) were associated with OMG CSR after thymectomy.

Conclusion: For OMG patients after thymectomy, RNS-positivity and histotype B2/B3 thymoma are independent predictors of conversion to GMG. On the other hand, thymic hyperplasia and stage I thymoma independently predict CSR.
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http://dx.doi.org/10.1186/s13023-022-02454-yDOI Listing
August 2022

Potential effects of virtual interventions for menopause management: a systematic review.

Menopause 2022 Aug 9. Epub 2022 Aug 9.

School of Nursing, Wenzhou Medical University, Wenzhou, China.

Importance: Menopausal women are one of the fastest growing demographic groups globally. Virtual interventions have emerged as alternate avenues for menopausal women to manage and cope with their symptoms.

Objective: The purpose of this review is to summarize existing research on the potential effects of virtual interventions for menopause management.

Evidence Review: This systematic review was written in accordance with Preferred Reporting Items for Systematic Reviews and Meta-Analyses guidelines. MEDLINE, PsychINFO, CINAHL, AgeLine, ERIC, ProQuest, Nursing and Allied Health Database, PsychARTICLES, and Sociology Database were used for literature search and searched from conception to December 2021. Original studies, including randomized controlled trials and quasi-experimental studies, were included if they evaluated a virtual intervention for menopause management and investigated the effects of these interventions on physical and psychosocial outcomes and/or the feasibility of these interventions among menopausal women. Included studies were published in peer-reviewed journals and assessed for quality using the Critical Appraisal Skills Program Checklists.

Findings: A total of 16 articles were included in this review. Virtual interventions have the potential to improve physical health outcomes including body weight/body mass index/waist circumference, pain, blood pressure, and cholesterol. However, conflicting results were identified for the outcomes of vasomotor and endocrine symptoms, sleep, and sexual functioning. Virtual interventions might also improve psychosocial outcomes, including knowledge and patient-physician communication, although conflicting results were again identified for treatment decision-making ability, quality of life, and anxiety and depression. Virtual interventions were feasible in terms of being usable and cost-effective, and eliciting satisfaction and compliance among menopausal women.

Conclusions And Relevance: Virtual interventions might have the potential to improve the physical and psychosocial health outcomes of menopausal women, although some conflicting findings arose. Future studies should focus on including diverse menopausal women and ethnic minorities, conducting research within low- to middle-income countries and communities, further exploring intervention design to incorporate features that are age and culture sensitive, and conducting full randomized controlled trials to evaluate the effects of the interventions.
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http://dx.doi.org/10.1097/GME.0000000000002020DOI Listing
August 2022

Right ventricle septal papillary muscle involvement and progressive enlargement in a patient with a rare case of isolated cardiac sarcoidosis.

Eur Heart J Cardiovasc Imaging 2022 Aug 9. Epub 2022 Aug 9.

Department of Magnetic Resonance, Lanzhou University Second Hospital, No. 82 Cuiyingmen, Chengguan District, Lanzhou 730030, China.

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http://dx.doi.org/10.1093/ehjci/jeac161DOI Listing
August 2022

Attachment avoidance and internalizing symptoms: Does respiratory sinus arrhythmia withdrawal make a difference?

J Affect Disord 2022 Aug 5. Epub 2022 Aug 5.

School of Psychology, Nanjing Normal University, PR China; School of Psychology, Shaanxi Normal University, PR China. Electronic address:

Objective: This study investigated the association between attachment avoidance and internalizing symptoms and the moderating role of parasympathetic nervous activity (indexed by respiratory sinus arrhythmia withdrawal) in the association.

Methods: A sample of 109 (M = 18.94 years old, SD = 0.92; 69 men) Chinese college students participated in this study. Participants reported attachment avoidance and internalizing symptoms, and their physiological data were collected.

Results: Results showed a positive link between attachment avoidance and internalizing symptoms. Further, respiratory sinus arrhythmia (RSA) withdrawal and attachment avoidance interactively predicted internalizing symptoms. Specifically, the positive relation between attachment avoidance and internalizing symptoms was only found among people of low, but not high, levels of RSA withdrawal.

Conclusion: Our study highlighted the importance of considering psychophysiological interactions in predicting internalizing symptoms in college students, and contributed to our understanding of the complicated factors underlying college students' internalizing problems.
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http://dx.doi.org/10.1016/j.jad.2022.07.070DOI Listing
August 2022

Radical recombination and antioxidants: a hypothesis on the FLASH effect mechanism.

Int J Radiat Biol 2022 Aug 10:1-9. Epub 2022 Aug 10.

Department of Engineering Physics, Tsinghua University, Beijing, China.

Purpose: FLASH (ultra-high dose rate) radiotherapy spares normal tissue while keeping tumor control. However, the mechanism of the FLASH effect remains unclear and may have consequences beyond the irradiated area.

Materials And Methods: We reanalyze the available results of ultra-high-dose-rate-related experiments to find out the key points of the mechanism of the FLASH effect. Then, we present a hypothesis on the mechanism of the FLASH effect: FLASH beams generate a high transient concentration of peroxyl radicals leading to a high fraction of radical recombination, which results in less oxidation damage to normal tissue. For the cells containing higher concentrations of antioxidants, the fractions of radical recombination are smaller because the antioxidants compete to react with peroxyl radicals. Therefore the damages by different dose rate beams differ slightly in this condition. Since some tumors contain a higher level of antioxidants, this may be the reason for the loss of the protective effect in tumors irradiated by FLASH beams. The high concentration of antioxidants in tumors results in slight radiolytic oxygen consumption, and consequently the protective effect observed in in vitro experiment cannot be observed in in vivo experiment. To quantitatively elaborate our hypothesis, a kinetic model is implemented to simulate the reactions induced by irradiation. Two parameters are defined to abstractly study the factors affecting the reaction, such as dose rate, antioxidants, total dose and reaction rate constants.

Results And Conclusions: We find that the explanation of the difference between in vivo and in vitro experiments is crucial to understanding the mechanism of the FLASH effect. Our hypothesis agrees with the results of related experiments. Based on the kinetic model, the effects of these factors on the FLASH effect are quantitatively investigated.
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http://dx.doi.org/10.1080/09553002.2022.2110307DOI Listing
August 2022

The Value of Dynamic Contrast-Enhanced Magnetic Resonance Imaging (DCE-MRI) in the Differentiation of Pseudoprogression and Recurrence of Intracranial Gliomas.

Contrast Media Mol Imaging 2022 22;2022:5680522. Epub 2022 Jul 22.

College of Medical Imaging, Shanxi Medical University, Taiyuan 030001, Shanxi Province, China.

Objective: The objective of this study was to determine the value of dynamic contrast-enhanced magnetic resonance imaging (DCE-MRI) in assessing postoperative changes in intracranial gliomas.

Method: A total of fifty-one patients who had new enhanced lesions after surgical resection followed by standard radiotherapy and chemotherapy were collected retrospectively from October 2014 to June 2021. The patients were divided into a pseudoprogression group (15 cases) and a recurrence group (36 cases) according to the pathological results of the second operation or a follow-up of more than six months. The follow-up data of all patients were complete, and DCE-MRI was performed. The images were processed to obtain the quantitative parameters , Ve, and Kep and the semiquantitative parameter iAUC, which were analysed with relevant statistical software.

Results: First, the difference in and iAUC values between the two groups was statistically significant (  ), and the difference in Ve and Kep values was not statistically significant (  ). Second, by comparing the area under the curve, threshold, sensitivity and specificity of , and iAUC, it was found that the iAUC threshold value was slightly higher than that of , and the specificity of was equal to that of iAUC, while the area under the curve and sensitivity of were higher than those of iAUC. Third, and iAUC had high accuracy in diagnosing recurrence and pseudoprogression, and had higher accuracy than iAUC.

Conclusion: In this study, DCE-MRI has a certain diagnostic value in the early differentiation of recurrence and pseudoprogression, offering a new method for the diagnosis and assessment of gliomas after surgery.
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http://dx.doi.org/10.1155/2022/5680522DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9337951PMC
August 2022

Iron overload induced osteocytes apoptosis and led to bone loss in Hepcidin mice through increasing sclerostin and RANKL/OPG.

Bone 2022 Aug 4;164:116511. Epub 2022 Aug 4.

Second Affiliated Hospital of Soochow University, Orthopedic Department, China; Osteoporosis Research Institute of Soochow University, China. Electronic address:

Background: Numerous studies have demonstrated that iron overload is a risk factor of osteoporosis. However, there has been no systematic and in-depth studies on the effect of iron overload on osteocytes and its role in iron overload-induced bone loss. Therefore, to address this problem, we carried out in vitro and in vivo studies using MLO-Y4 osteocyte-like cells and Hepcidin mice as iron overload models.

Methods: (1) MLO-Y4 cells were treated with ferric ammonium citrate (FAC). Intracellular reactive oxygen species (ROS) levels and apoptosis of MLO-Y4 cells were determined by flow cytometry. Western blotting was performed to evaluate the effect of FAC on the expression of sclerostin and RANKL/OPG. (2) The conditioned medium of MLO-Y4 cells after treatment with FAC was collected and used to treat pre-osteoblasts and monocytes. Alkaline phosphatase (ALP) staining and alizarin red (AR) staining were used to evaluate osteogenic differentiation capacity, and tartrate-resistant acid phosphatase (TRAP) staining was performed to demonstrate osteoclast differentiation capacity. (3) In vivo studies included a wild type mouse, Hepcidin mice, Hepcidin mice + deferoxamine (DFO), and Hepcidin mice + N-actyl-l-cysteine (NAC) group. Micro-CT was performed to evaluate the bone mineral density (BMD), bone volume, and bone micro-architecture of the mice, and three bending tests were used to assess bone strength. Histological analysis was used to detect alterations in bone turnover. TUNEL staining and scanning electron microscopy (SEM) were performed to evaluate the apoptosis and morphology of osteocytes. Immunohistochemical staining and Western blotting were used to determine alterations in sclerostin and RANKL/OPG expression levels in mice.

Results: (1) FAC increased intracellular ROS and apoptosis in MLO-Y4 cells, while FAC enhanced the expression of sclerostin and RANKL/OPG in MLO-Y4 cells. (2) Conditioned medium of MLO-Y4 cells inhibited the osteogenic capacity of osteoblasts while stimulating osteoclast differentiation. (3) By increasing oxidative stress, iron overload promotes the apoptosis of osteocytes and undermines the morphology of osteocytes in Hepcidin mice, further increasing the expression levels of sclerostin and RANKL/OPG in osteocytes, which is considered to be the causative factor for reduced bone formation and enhanced bone resorption. DFO administration reduced iron levels, and NAC treatment decreased oxidative stress in Hepcidin mice. Therefore, DFO or NAC treatment rescued the decrease in BMD, bone volume, and bone strength and attenuated the deterioration of bone architecture in Hepcidin mice by attenuating the effect of iron overload on osteocytes.

Conclusion: Osteocyte apoptosis due to increased ROS and resultant sclerostin and RANKL/OPG expression alteration was the main reason for bone loss in Hepcidin mice. Osteocytes are the main targets for the prevention and treatment of iron overload-induced osteoporosis.
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http://dx.doi.org/10.1016/j.bone.2022.116511DOI Listing
August 2022

DNA methylation in transposable elements buffers the connection between three-dimensional chromatin organization and gene transcription upon rice genome duplication.

J Adv Res 2022 Aug 4. Epub 2022 Aug 4.

Shanghai Collaborative Innovation Center of Agri-Seeds / School of Agriculture and Biology, Shanghai Jiao Tong University, Shanghai 200240, China. Electronic address:

Introduction: Polyploidy is a major force in plant evolution and the domestication of cultivated crops.

Objectives: The study aimed to explore the relationship and underlying mechanism between three-dimensional (3D) chromatin organization and gene transcription upon rice genome duplication.

Methods: The 3D chromatin structures between diploid (2C) and autotetraploid (4C) rice were compared using high-throughput chromosome conformation capture (Hi-C) analysis. The study combined genetics, transcriptomics, whole-genome bisulfite sequencing (WGBS-seq) and 3D genomics approaches to uncover the mechanism for DNA methylation in modulating gene transcription through 3D chromatin architectures upon rice genome duplication.

Results: We found that 4C rice presents weakened intra-chromosomal interactions compared to its 2C progenitor in some chromosomes. In addition, we found that changes of 3D chromatin organizations including chromatin compartments, topologically associating domains (TADs), and loops, are uncorrelated with gene transcription. Moreover, DNA methylations in the regulatory sequences of genes in compartment A/B switched regions and TAD boundaries are unrelated to their expression. Importantly, although there was no significant difference in the methylation levels in transposable elements (TEs) in differentially expressed gene (DEG) and non-DEG promoters between 2C and 4C rice, we found that the hypermethylated TEs across genes in compartment A/B switched regions and TAD boundaries may suppress the expression of these genes.

Conclusion: The study proposed that the rice genome doubling might modulate TE methylation to buffer the effects of chromatin architecture on gene transcription in compartment A/B switched regions and TAD boundaries, resulting in the disconnection between 3D chromatin structure alteration and gene transcription upon rice genome duplication.
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http://dx.doi.org/10.1016/j.jare.2022.07.007DOI Listing
August 2022

Pure Ankle Dislocation Without Associated Fracture: A Series of Cases and Our Clinical Experience.

Indian J Orthop 2022 Aug 27;56(8):1370-1377. Epub 2022 Jun 27.

Department of Orthopedics, Orthopedic Research Institute, West China Hospital, Sichuan University, No.37, Guoxue Avenue, Chengdu, 610041 Sichuan Province China.

Purpose: Pure ankle dislocation without associated fracture is extremely rare. The current study reports eight cases of this type of injury, aiming to add some new material to the published data and present our clinical experience.

Methods: Six open and two closed posteromedial tibiotalar dislocations without associated fractures treated in our department from 2015 to 2019 were retrospectively analyzed. Emergent reduction was performed for all patients followed by an average of 6 weeks of immobilization with external fixators in open cases and a short leg cast in closed cases. No patients underwent ligament or capsule repair except one case. The clinical data were recorded and analyzed.

Results: At a mean follow-up of 33 months (range 13-61), the average AOFAS score was 91 (range 78-100) with five ankles rated as excellent and three rated as good. All of the eight patients returned to their prior daily life and the two closed patients with sports injuries resumed pre-injury activity level. The average range of motion (ROM) loss of the ankles was 9 degrees for plantarflexion and 3 degrees for dorsiflexion. Complications included superficial infection, moderate ankle stiffness, ankle degenerative change and residual numbness. None of the eight patients showed obvious ankle instability.

Conclusion: Pure ankle dislocation without associated fracture is a rare injury. Emergent reduction, appropriate wound care in open injuries and proper joint stabilisation with a cast or external fixator provide good clinical results and could be the mainstay of treatment.
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http://dx.doi.org/10.1007/s43465-022-00682-9DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9283584PMC
August 2022

influences B cell development and function in mouse.

Haematologica 2022 Aug 4. Epub 2022 Aug 4.

Department of Pharmaceutical Sciences, St. Jude Children's Research Hospital, Memphis, TN, USA; Department of Oncology, St. Jude Children's Research Hospital, Memphis, TN.

There is growing evidence for an inherited basis of susceptibility to childhood acute lymphoblastic leukemia (ALL). Genome-wide association studies by us and others have identified non-coding ALL risk variants at the ARID5B gene locus, but the molecular mechanisms linking ARID5B to normal and malignant hematopoiesis remain largely unknown. Using a Vav1-driven transgenic mouse model, we characterized the role of Arid5b in hematopoiesis in vivo. Arid5b overexpression resulted in a dramatic reduction in the proportion of circulating B cells, immature, and mature B-cell fractions in the peripheral blood and the bone marrow, and also decrease of follicular B cells in the spleen. There were significant defects in Bcell activation upon Arid5b overexpression in vitro with hyperactivation of the B-cell receptor signaling at baseline. In addition, increased mitochondrial oxygen consumption rate of naïve or stimulated B cells of Arid5bOE mice was observed, compared to wildtype counterparts. Taken together, our results indicate that ARID5B may play important role in B-cell development and function.
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http://dx.doi.org/10.3324/haematol.2022.281157DOI Listing
August 2022

Melanoma-derived small extracellular vesicles remodel the systemic onco-immunity via disrupting hematopoietic stem cell proliferation and differentiation.

Cancer Lett 2022 Jul 31;545:215841. Epub 2022 Jul 31.

Affiliated Cancer Hospital & Institute of Guangzhou Medical University, Guangzhou, 510095, China; Guangzhou Municipal and Guangdong Provincial Key Laboratory of Protein Modification and Degradation, State Key Laboratory of Respiratory Disease, School of Basic Medical Sciences, Guangzhou Medical University, Guangzhou, 511436, China. Electronic address:

Hematopoiesis and the immune system beyond the tumor microenvironment are typically dysregulated in cancer. Tumor-derived small extracellular vesicles (sEVs) containing exosomes are emerging contributors to tumor progression and immunomodulation. However, a comprehensive definition of how tumor-derived sEVs impacts systemic immunity is lacking. In this study, we used mass cytometry with extensive antibody panels to determine the expression of 24 immune cell markers, eight intracellular proteins, and seven immune checkpoint proteins in systemic immune cell lineages. The systemic immune landscape in response to tumor-derived sEVs across three immune organs in a melanoma mouse model was then characterized. Melanoma-derived sEVs significantly and extensively influenced the composition and intracellular pathways of immune lineage and T cells. An immunosuppressive immune system with decreased natural killer and CD8 T cells in the spleen and bone marrow (BM), increased regulatory T cells in lymph nodes, and increased polymorphonuclear myeloid-derived suppressor cells (PMN-MDSC) in the BM, was induced by melanoma-derived sEVs. Additionally, melanoma-derived sEVs significantly enhanced the PD-1/PD-L1 axis in CD4 T cells and myeloid cell subsets. These sEVs largely promoted the proliferation of multiple hematopoietic stem and progenitor cell subsets and accelerated their differentiation towards MDSCs in naive mice and mice undergoing hematopoietic reconstruction. Moreover, melanoma-derived sEVs directly promoted the survival and activation of MDSCs in vitro. Collectively, our work examines the effects of tumor-derived sEVs on the systemic onco-immune macroenvironments and highlights the contribution of these sEVs to the dysregulation of hematopoiesis and systemic immune landscape in cancer.
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http://dx.doi.org/10.1016/j.canlet.2022.215841DOI Listing
July 2022

Study on Size Estimation of Prosthesis in Rhinoplasty Based on Mechanical Test and Analysis.

J Craniofac Surg 2022 Aug 3. Epub 2022 Aug 3.

Sea-maid Cosmetic Clinic, Xi'an City, Shaanxi Province, China.

Rhinoplasty is a common plastic surgery. The determination of prosthesis size for rhinoplasty has long been dependent on the surgeon's experience. Some of the patients suffered from complications after the surgery. In this paper, we present a method for estimating the size of prosthesis in rhinoplasty. A nasal tension tester is designed and implemented. On the basis of the mechanical theory of Hyperelastic Materials, an algorithm for inverse calculation of material parameters is proposed; On the basis of the material parameters, an algorithm for estimating the safety range of nasal stretch is established. The nasal tension tester can obtain the nasal tension data without harming the subject. Our results show that different subjects generally have different nasal skin elasticity and tolerance to skin stretch. Therefore, the selection of prosthesis size for rhinoplasty should not be generalized, but should be tested and calculated separately for each subject. The results of this study are helpful for doctors to quantitatively understand the skin condition of the subject 's nose and the safe range of nasal stretch, based on which the determination of prosthesis size can be made for rhinoplasty.
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http://dx.doi.org/10.1097/SCS.0000000000008822DOI Listing
August 2022

Epidemiological studies of accessory cardiac bronchus and a new variant.

Quant Imaging Med Surg 2022 Aug;12(8):4296-4303

Department of Radiology, Hebei General Hospital, Shijiazhuang, China.

Accessory cardiac bronchus (ACB) is a rare tracheobronchial branching abnormality which originates from the medial wall of the intermediate or main bronchus and is directed to the heart. Three types of ACB have been recognized: type (a) is similar to a short diverticulum, type (b) is a long bronchus ventilating a small undeveloped lobule, and type (c) is an intermediate type with a long diverticulum but no bronchial or alveolar arborization. Herein, we report 40 consecutive cases of ACB detected in 10,287 routine spiral computed tomography (CT) examinations of the chest. The frequency of the anomaly was 0.39%. The study included 17 females and 23 males (female to male ratio 1:1.35). A total of 24 cases belonged to type (a), 14 cases were type (b), and 2 cases were type (c). The mean largest diameter of ACB was 7.9 (range, 4.0 to 12.0) mm and the mean length was 7.5 (range, 3.0 to 18.0) mm. The mean ratio of the largest diameter to length was 1.1, the ratio for (a) was often greater than 1, and the ratios of (b) and (c) were often less than 1. The ACB originated from the intermediate bronchus in 29 cases, which accounted for the largest proportion. The ACB originated from the basal bronchus of the lower lobe in 11 cases (6 cases from the right lower lobe and 5 from the left), which has never been reported before and may be a new variant.
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http://dx.doi.org/10.21037/qims-22-68DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9338377PMC
August 2022

News Coverage of Abortion in Relation to Race and Class in the United States in 2021.

Am J Bioeth 2022 08;22(8):88-90

Henan Provincial People's Hospital, People's Hospital of Zhengzhou University, People's Hospital of Henan University.

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http://dx.doi.org/10.1080/15265161.2022.2089279DOI Listing
August 2022

Iron controls T helper cell pathogenicity by promoting glucose metabolism in autoimmune myopathy.

Clin Transl Med 2022 Aug;12(8):e999

Department of Rheumatology, The First Affiliated Hospital, Sun Yat-sen University, Guangzhou, China.

Background: T helper cells in patients with autoimmune disease of idiopathic inflammatory myopathies (IIM) are characterized with the proinflammatory phenotypes. The underlying mechanisms remain unknown.

Methods: RNA sequencing was performed for differential expression genes. Gene expression in CD4 T-cells was confirmed by quantitative real-time PCR. CD4 T-cells from IIM patients or healthy controls were evaluated for metabolic activities by Seahorse assay. Glucose uptake, T-cell proliferation and differentiation were evaluated and measured by flow cytometry. Human CD4 T-cells treated with iron chelators or Pfkfb4 siRNA were measured for glucose metabolism, proliferation and differentiation. Signalling pathway activation was evaluated by western blot and flow cytometry. Mouse model of experimental autoimmune myositis (EAM) were induced and treated with iron chelator or rapamycin. CD4 T-cell differentiation and muscle inflammation in the EAM mice were evaluated.

Results: RNA-sequencing analysis revealed that iron was involved with glucose metabolism and CD4 T-cell differentiation. IIM patient-derived CD4 T-cells showed enhanced glycolysis and mitochondrial respiration, which was inhibited by iron chelation. CD4 T-cells from patients with IIM was proinflammatory and iron chelation suppressed the differentiation of interferon gamma (IFNγ)- and interleukin (IL)-17A-producing CD4 T-cells, which resulted in an increased percentage of regulatory T (Treg) cells. Mechanistically, iron promoted glucose metabolism by an upregulation of PFKFB4 through AKT-mTOR signalling pathway. Notably, the knockdown of Pfkfb4 decreased glucose influx and thus suppressed the differentiation of IFNγ- and IL-17A-producing CD4 T-cells. In vivo, iron chelation inhibited mTOR signalling pathway and reduced PFKFB4 expression in CD4 T-cells, resulting in reduced proinflammatory IFNγ- and IL-17A-producing CD4 T-cells and increased Foxp3 Treg cells, leading to ameliorated muscle inflammation.

Conclusions: Iron directs CD4 T-cells into a proinflammatory phenotype by enhancing glucose metabolism. Therapeutic targeting of iron metabolism should have the potential to normalize glucose metabolism in CD4 T-cells and reverse their proinflammatory phenotype in IIM.
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http://dx.doi.org/10.1002/ctm2.999DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9345506PMC
August 2022

Metformin Alleviates LPS-Induced Acute Lung Injury by Regulating the SIRT1/NF-κB/NLRP3 Pathway and Inhibiting Endothelial Cell Pyroptosis.

Front Pharmacol 2022 15;13:801337. Epub 2022 Jul 15.

Department of Anesthesiology and Surgical Intensive Care Unit, Xinhua Hospital Affiliated to Shanghai Jiaotong University School of Medicine, Shanghai, China.

Acute respiratory distress syndrome (ARDS), a devastating complication of numerous conditions, is often associated with high mortality. It is well known that endothelial cell (EC) damage and inflammation are vital processes in the pathogenesis of ARDS. Nevertheless, the mechanisms of EC damage are largely unknown. In the present study, we investigated the role of pyroptosis in the initiation of ARDS and demonstrated that endothelial pyroptosis might play a pivotal role in the pathophysiology of ARDS. Metformin, an antidiabetic drug, exhibited a protective effect in lipopolysaccharide (LPS)-induced lung injury, and we hypothesized that metformin alleviated LPS-induced lung injury inhibiting ECs pyroptosis. , male ICR mice were intratracheally injected with LPS, and metformin was previously administered intraperitoneally. Morphological properties of lung tissues were detected. We showed that metformin inhibited NLRP3 inflammasome activation and NLRP3-stimulated pyroptosis induction, as shown by decreased levels of cleaved caspase-1, N-terminal fragment of GSDMD, and protein contents of IL-1β in lung tissues of mice exposed to LPS. LPS-induced expression of vascular adhesion molecules was also reduced after the treatment with metformin. , exposure of pulmonary ECs to LPS resulted in increased expression of NLRP3 and pyroptosis-associated indicators. By inhibiting the expression of NLRP3 with NLRP3 inhibitor MCC950, pyroptosis-related markers and vascular adhesion molecules were ameliorated. Moreover, metformin treatment significantly inhibited the NF-κB signaling pathway and increased the expression of sirtuin 1 (SIRT1) both in LPS-stimulated lung tissues and pulmonary ECs. Administration of the selective SIRT1 inhibitor nicotinamide significantly reversed the protective effect of metformin against endothelial pyroptosis and lung injury in LPS-treated ECs and LPS-induced acute lung injury (ALI). Thus, these findings demonstrated that metformin alleviated LPS-induced ALI by inhibiting NF-κB-NLRP3-mediated ECs pyroptosis, possibly by upregulating the expression of SIRT1.
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http://dx.doi.org/10.3389/fphar.2022.801337DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9334876PMC
July 2022

Recent Advances in Multifunctional Mechanical-Chemical Superhydrophobic Materials.

Front Bioeng Biotechnol 2022 13;10:947327. Epub 2022 Jul 13.

China State Key Laboratory of Biogeology and Environmental Geology, Engineering Research Center of Nano-Geomaterials of Ministry of Education, Faculty of Materials Science and Chemistry, China University of Geosciences, Wuhan, China.

In recent years, biology-inspired superhydrophobic technology has attracted extensive attention and has been widely used in self-cleaning, anti-icing, oil-water separation, and other fields. However, the poor durability restricts its application in practice; thus, it is urgent to systematically summarize it so that scientists can guide the future development of this field. Here, in this review, we first elucidated five kinds of typical superhydrophobic models, namely, Young's equation, Wenzel, Cassie-Baxter, Wenzel-Cassie, "Lotus," and "Gecko" models. Then, we summarized the improvement in mechanical stability and chemical stability of superhydrophobic surface. Later, the durability test methods such as mechanical test methods and chemical test methods are discussed. Afterwards, we displayed the applications of multifunctional mechanical-chemical superhydrophobic materials, namely, anti-fogging, self-cleaning, oil-water separation, antibacterial, membrane distillation, battery, and anti-icing. Finally, the outlook and challenge of mechanical-chemical superhydrophobic materials are highlighted.
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http://dx.doi.org/10.3389/fbioe.2022.947327DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9326238PMC
July 2022

A Review of Pharmaceutical Robot based on Hyperspectral Technology.

J Intell Robot Syst 2022 22;105(4):75. Epub 2022 Jul 22.

National Engineering Laboratory of Robot Visual Perception and Control Technology, Hunan University, Changsha, 410082 Hunan People's Republic of China.

The quality and safety of medicinal products are related to patients' lives and health. Therefore, quality inspection takes a key role in the pharmaceutical industry. Most of the previous solutions are based on machine vision, however, their performance is limited by the RGB sensor. The pharmaceutical visual inspection robot combined with hyperspectral imaging technology is becoming a new trend in the high-end medical quality inspection process since the hyperspectral data can provide spectral information with spatial knowledge. Yet, there is no comprehensive review about hyperspectral imaging-based medicinal products inspection. This paper focuses on the pivotal pharmaceutical applications, including counterfeit drugs detection, active component analysis of tables, and quality testing of herbal medicines and other medical materials. We discuss the technology and hardware of Raman spectroscopy and hyperspectral imaging, firstly. Furthermore, we review these technologies in pharmaceutical scenarios. Finally, the development tendency and prospect of hyperspectral imaging technology-based robots in the field of pharmaceutical quality inspection is summarized.
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http://dx.doi.org/10.1007/s10846-022-01602-7DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9306415PMC
July 2022

Eye movements as a measure of word comprehension deficits in primary progressive aphasia.

Brain Lang 2022 09 28;232:105165. Epub 2022 Jul 28.

Mesulam Center for Cognitive Neurology and Alzheimer's Disease, Northwestern University Feinberg School of Medicine, Chicago, IL, USA; Department of Neurology, Northwestern University Feinberg School of Medicine, Chicago, IL, USA.

Introduction: Eye movement studies can uncover subtle aspects of language processing impairment in individuals with primary progressive aphasia (PPA), who may have difficulty understanding words. This study examined eye movement patterns on a word-object matching task in response to varying levels of word-knowledge in PPA.

Methods: Participants with semantic and non-semantic PPA completed an object-matching task, where a word was presented and participants then selected the corresponding pictured object from an array. Afterwards, participants defined words for trials to which they incorrectly pointed. Linear mixed-effects analyses examined fixation differences on targets and related and unrelated foils.

Results: On incorrectly-pointed trials, participants demonstrated greater fixation duration on related foils, demonstrating intra-category blurring. For words that could not be defined, there was similar fixation duration on related and unrelated foils, demonstrating inter-category semantic blurring.

Discussion: This study demonstrated that fixation patterns reflect varying levels of word knowledge in PPA.
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http://dx.doi.org/10.1016/j.bandl.2022.105165DOI Listing
September 2022

The effects of Baduanjin and yoga exercise programs on physical and mental health in patients with Multiple Sclerosis: A randomized controlled trial.

Complement Ther Med 2022 Jul 26;70:102862. Epub 2022 Jul 26.

Department of Anesthesiology, Northern Jiangsu People's Hospital, Yangzhou, China; Clinical Medical College, Yangzhou University, Yangzhou, China. Electronic address:

Objective: To discuss whether Baduanjin and yoga exercise interventions improve motor function, posture control, and relieving fatigue and depression in MS patients. And to explore whether practicing Baduanjin benefits MS patients more than yoga.

Design: A prospective, randomized, controlled, three-arm trial comparing BDJ (n = 30), yoga (n = 30) and control group (n = 20).

Setting: Jiangsu Provincial Corps Hospital.

Intervention: Eligible participants were randomized to a 24-week Baduanjin or yoga intervention, or a usual activity control group. Balance, posture control and trunk movement were measured with the Berg Balance Scale (BBS) and Trunk Impairment Scale (TIS). Fatigue was measured using the Fatigue Severity Scale (FSS) and depressive symptoms via the Zung Self-Rating Depression Scale (SDS).

Results: For BBS and TIS, there were significant changes pre- to post- exercise in two exercise groups (P < 0.05), with greater increases in the Baduanjin exercise group (BDJ group). For the FSS, there were significant changes pre- to post- exercise in both the BDJ (P = 0.0292) and yoga groups (P = 0.0150). For the SDS, the pre- and post-exercise difference of the BDJ group was larger than the yoga group (P < 0.0001). On the other hand, we could not find any changes of the BBS, TIS, FSS, and SDS scores in the control group (p > 0.05).

Conclusion: The results suggest that practicing Baduanjin was more effective than yoga and that it is suitable for the MS patients.
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http://dx.doi.org/10.1016/j.ctim.2022.102862DOI Listing
July 2022

Soy oil and SPI based-oleogels structuring with glycerol monolaurate by emulsion-templated approach: Preparation, characterization and potential application.

Food Chem 2022 Jul 25;397:133767. Epub 2022 Jul 25.

Zhejiang Key Laboratory for Agro-Food Processing, College of Biosystems Engineering and Food Science, Zhejiang University, Hangzhou 310058, China; Ningbo Research Institute, Zhejiang University, Ningbo 315100, China. Electronic address:

In this study, soybean oil-based oleogels were prepared using soy-protein isolate (SPI) and glycerol monolaurate (GML) in an emulsion-template approach. The rheological, texture, microstructure, and oil-retention properties of the obtained oleogels were analyzed. Results showed that the soy oil-based oleogel prepared with 6 wt% GML exhibited high oil loss, low-hardness, and needle-like morphology compared to the soy-oil/SPI-based oleogel. On the other hand, soy oil-based /SPI-based oleogels structured by 3 or 6 wt% GML presented moderate thermal-stability and lowest oil loss than those prepared without GML. Furthermore, SPI-based oleogel containing 6 wt% GML showed highest free fatty acids release (62.07%) with significantly improved elastic modulus and apparent viscosity. Additionally, the obtained oleogels displayed the occurrence of van der Waals interactions and intermolecular hydrogen bonds, presenting enhanced thermal stability. These results contribute to a better understanding of oleogelation-based emulsions for formulating trans-free and low-saturated foodstuffs with desired physical and functional properties.
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http://dx.doi.org/10.1016/j.foodchem.2022.133767DOI Listing
July 2022

A retrospective cohort analysis of the Yale pediatric genomics discovery program.

Am J Med Genet A 2022 Jul 28. Epub 2022 Jul 28.

Pediatric Genomics Discovery Program, Department of Pediatrics, Yale University School of Medicine, New Haven, Connecticut, USA.

The Pediatric Genomics Discovery Program (PGDP) at Yale uses next-generation sequencing (NGS) and translational research to evaluate complex patients with a wide range of phenotypes suspected to have rare genetic diseases. We conducted a retrospective cohort analysis of 356 PGDP probands evaluated between June 2015 and July 2020, querying our database for participant demographics, clinical characteristics, NGS results, and diagnostic and research findings. The three most common phenotypes among the entire studied cohort (n = 356) were immune system abnormalities (n = 105, 29%), syndromic or multisystem disease (n = 103, 29%), and cardiovascular system abnormalities (n = 62, 17%). Of 216 patients with final classifications, 77 (36%) received new diagnoses and 139 (64%) were undiagnosed; the remaining 140 patients were still actively being investigated. Monogenetic diagnoses were found in 67 (89%); the largest group had variants in known disease genes but with new contributions such as novel variants (n = 31, 40%) or expanded phenotypes (n = 14, 18%). Finally, five PGDP diagnoses (8%) were suggestive of novel gene-to-phenotype relationships. A broad range of patients can benefit from single subject studies combining NGS and functional molecular analyses. All pediatric providers should consider further genetics evaluations for patients lacking precise molecular diagnoses.
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http://dx.doi.org/10.1002/ajmg.a.62918DOI Listing
July 2022

Molecular Detection of Zoonotic and Veterinary Pathogenic Bacteria in Pet Dogs and Their Parasitizing Ticks in Junggar Basin, North-Western China.

Front Vet Sci 2022 8;9:895140. Epub 2022 Jul 8.

State International Joint Research Center for Animal Health Breeding, College of Animal Science and Technology, Shihezi University, Shihezi, China.

Despite the recognized epidemiological importance of ticks as vectors for pathogens that cause numerous zoonotic and veterinary diseases, data regarding the pathogens of pet dogs and their parasitic ticks in the Junggar Basin are scarce. In this study, a total of 178 blood samples and 436 parasitic ticks were collected from pet dogs in Junggar Basin, Xinjiang Uygur Autonomous Region (XUAR), north-western China. All ticks were identified as sensu stricto (s.s.) according to morphological and molecular characteristics. s.s. ticks were collected from pet dogs in China for the first time. Seven tick-borne pathogens, such as R. barbariae, spp., , and , were detected from ticks, whereas the first five bacteria were detected from blood samples of dogs. spp. was the most predominant pathogen in both blood samples and ticks of pet dogs, with the detection rates of 16.29 and 16.74%, respectively. Moreover, 17 ticks and 1 blood sample were co-infected with two pathogens, and 1 tick was co-infected with three pathogens. This study provided molecular evidence for the occurrence of spp., spp., spp., and spp. circulating in pet dogs and their parasitic ticks in Junggar Basin, north-western China. These findings extend our knowledge of the tick-borne pathogens in pet dogs and their parasitic ticks in Central Asia; therefore, further research on these pathogens and their role in human and animal diseases is required.
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http://dx.doi.org/10.3389/fvets.2022.895140DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9311330PMC
July 2022

Platelet-rich plasma pretreatment protects anterior cruciate ligament fibroblasts correlated with PI3K-Akt-mTOR pathway under hypoxia condition.

J Orthop Translat 2022 May 10;34:102-112. Epub 2022 Mar 10.

Department of Orthopaedics and Traumatology, The Chinese University of Hong Kong, Hong Kong, China.

Background: /Objective: Biological factors such as platelet-rich plasma (PRP) combined with anterior cruciate ligament (ACL) primary repair technology are used to treat ACL injury. However, the protective mechanism of PRP for ACL fibroblasts under hypoxia condition is still unknown. The aim of this study was to investigate the protective effect of PRP on ACL fibroblasts under hypoxia condition and illustrate the mechanism of PRP regulating the ACL fibroblasts under hypoxia condition.

Methods: The cells were divided into three groups: control group, hypoxia group and PRP pretreatment group. Lethal dose (LD) 50 for hypoxia induction time and the maximum efficacy of PRP concentration were confirmed by CCK-8 assay. The ability of cell apoptosis, cell proliferation, and cell migration were tested by flow cytometry, scratch assay and transwell assay, respectively. Extracellular matrix (ECM) synthesis and hypoxia-inducible factor 1α (HIF-1α) were identified by immunofluorescence staining, Masson's staining and transmission electron microscope analysis. Inflammatory cell infiltration was assessed by hematoxylin and eosin staining as well as immunofluorescence staining. Western blot analysis and real-time PCR were performed to assess the associated gene and protein expression, respectively. The ratio of phosphorylated/total PI3K, Akt and mTOR were also assessed by western blot analysis.

Results: ① LD 50 of hypoxia was 48 ​h and the maximum efficacy of PRP concentration was 600 ​× ​10/L. ② ANNEXIN V-FITC/PI flow cytometry showed that the hypoxia condition significantly increased the apoptosis of cells (P ​< ​0.001) whereas PRP pretreatment significantly decreased the apoptosis of cells under hypoxia (P ​< ​0.001). The expressions of gene and protein of Bax, Bcl-2, cleaved-caspase 3 were consistent with the results of flow cytometric analysis. ③ Cell cycle analysis for flow cytometry showed the inhibitory effect of hypoxia and promotive effect of PRP pretreatment. ④ Immunofluorescence staining (HIF-1α, collagen I and III) showed the positive effect of hypoxia and negative effect of PRP on these parameters. Real-time PCR showed that type I and III collagen were 2.1 folds and 2.5 folds higher after 48 ​h hypoxia induction compared to the control group. PRP pretreatment significantly reduced the type I and III collagen mRNA expression of the hypoxia induced ACL fibroblasts to 78.5% and 77.7% at 48 ​h compared to hypoxia group (P ​< ​0.001), respectively.⑤ Cell migration assay showed that hypoxia condition significantly restrained cell migration compared with the control group. PRP could alleviate the inhibitory effect of hypoxia on fibroblasts. ⑥ Western blot analysis showed the ratio of phosphorylated/total PI3K, Akt and mTOR in hypoxia group increased to 31%, 20% and 44/% compared to control group, respectively. ⑦ The results of in vivo analysis was in accordance with the results of in vitro analysis.

Conclusion: PRP can protect ACL fibroblasts via decreasing apoptosis and increasing cell viability, cell migration and cell proliferation under hypoxia condition. And such PRP protective effect was correlated with PI3K/Akt/mTOR pathway.

The Translational Potential Of This Article: PRP can be used to treat patients with ACL tear by injection under arthroscopy or ultrasound guiding.
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http://dx.doi.org/10.1016/j.jot.2022.02.002DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9283994PMC
May 2022

Self-Supply Oxygen ROS Reactor via Fenton-like Reaction and Modulating Glutathione for Amplified Cancer Therapy Effect.

Nanomaterials (Basel) 2022 Jul 21;12(14). Epub 2022 Jul 21.

Key Laboratory for Photonic and Electronic Bandgap Materials, Ministry of Education, College of Chemistry & Chemical Engineering, Harbin Normal University, Harbin 150025, China.

Reactive oxygen species (ROS) are highly reactive oxidant molecules that can kill cancer cells through irreversible damage to biomacromolecules. ROS-mediated cancer therapies, such as chemodynamic (CDT) and photodynamic therapy (PDT), are often limited by the hypoxia tumor microenvironment (TME) with high glutathione (GSH) level. This paper reported the preparation, characterization, in vitro and in vivo antitumor bioactivity of a meso-tetra(4-carboxyphenyl)porphine (TCPP)-based therapeutic nanoplatform (CMMFTP) to overcome the limitations of TME. Using Cu as the central ion and TCPP as the ligand, the 2D metal-organic framework Cu-TCPP was synthesized by the solvothermal method, then CMMFTP was prepared by modifying MnO, folic acid (FA), triphenylphosphine (TPP), and poly (allylamine hydrochloride) (PAH) on the surface of Cu-TCPP MOFs. CMMFTP was designed as a self-oxygenating ROS nanoreactor based on the PDT process of TCPP MOFs and the CDT process by Cu(II) and MnO components (mainly through Fenton-like reaction). The in vitro assay suggested CMMFTP caused a 96% lethality rate against Hela cells (MTT analysis) in specific response to TME stimulation. Moreover, the Cu(II) and MnO in CMMFTP efficiently depleted the glutathione (80%) in tumor cells and consequently amplified ROS levels to improve CDT/PDT effects. The FA-induced tumor targeting and TPP-induced mitochondria targeting further enhanced the antitumor activity. Therefore, the nanoreactor based on dual targeting and self-oxygenation-enhanced ROS mechanism provided a new strategy for cancer therapy.
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http://dx.doi.org/10.3390/nano12142509DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9319594PMC
July 2022

Purification and Identification of Flavonoid Molecules from Waste Extracts and Evaluation of Antioxidant, Antiproliferative and Antimicrobial Activities.

Molecules 2022 Jul 8;27(14). Epub 2022 Jul 8.

Shanghai Key Laboratory of Functional Materials Chemistry, School of Chemistry and Molecular Engineering, East China University of Science and Technology, Shanghai 200237, China.

is widely used in the essential oil industry and generates large amounts of waste annually. The purpose of this research is the recycling of bioactive flavonoids from rose waste biomass to develop high-value products. Resin screening and adsorption/desorption dynamic analysis showed that HP20 resin was suitable to purify the flavonoids from waste extracts. Under the optimal enrichment process, the product had a 10.7-fold higher purity of flavonoids with a satisfactory recovery of 82.02%. In total, 14 flavonoids were identified in the sample after purification by UHPLC-QTOF-MS. Moreover, the DPPH and ABTS assays revealed that the flavonoids-purified extracts exhibited higher antioxidant activities than the crude extracts. Meanwhile, the purified extracts presented stronger antiproliferative activity against HepG2, Caco-2, MCF-7 and A549 cell lines. The bacteriostatic effects of the purified extracts against four bacteria (, , , )) and yeast ()) were stronger compared with the crude extracts. It was concluded that flavonoids-enriched extracts from waste had the potential to be applied in functional food and pharmaceutical industries.
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http://dx.doi.org/10.3390/molecules27144379DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9323010PMC
July 2022
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