Publications by authors named "Hui Zeng"

603 Publications

Multivariate Synergistic Flexible Metal-Organic Frameworks with Superproton Conductivity for Direct Methanol Fuel Cells.

Angew Chem Int Ed Engl 2021 Oct 14. Epub 2021 Oct 14.

Jilin University, College of Chemistry, CHINA.

Improving proton conductivity and fabricating viable metal-organic frameworks (MOFs) based proton exchange membranes (PEMs) are central issues exploiting electrolyte MOFs. We aim to design multivariate flexibility synergistic strategy to achieve Flexible MOFs (FMOFs) with high conductivity at a wide range of humidity. In situ powder X-ray diffraction (PXRD)  and temperature-dependent Fourier transform infrared spectra (FT-IR) prove the synergistic self-adaption between dynamic torsion of alkyl sulfonic acid and dynamic breathing of FMOF, forming a continuous hydrogen-bonding networks to maintain high conductivity. Based on the convincing proton conductivity, we construct a series of long-term durable MOF-based PEMs that serve as a bridge between MOF and fuel cell. Consequently, the membrane electrode assembly (MEA) of the flexible PMNS1-40 exhibits a maximum single-cell power density of 34.76 mW/cm 2  and hopefully opens doors to evaluate the practical application of proton-conducting MOFs in direct methanol fuel cells.
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http://dx.doi.org/10.1002/anie.202112922DOI Listing
October 2021

Morphological and metabolic changes in an aged strain of Agaricus bisporus As2796.

Appl Microbiol Biotechnol 2021 Oct 1;105(20):7997-8007. Epub 2021 Oct 1.

Institute of Edible Mushroom, Fujian Academy of Agricultural Sciences, National-Local Joint Engineering Research Center for Breeding and Cultivation of Featured Edible Mushroom, No. 104, Pudang, Jin'an District, Fujian Province, 350011, Fuzhou, China.

Agaricus bisporus is the most widely cultivated edible mushroom in the world. Strain quality has an important influence on the yield of A. bisporus, with strains that exhibit aging being a common problem during cultivation. However, little is known about the aging mechanisms of A. bisporus strain. In this study, the normal A. bisporus As2796 strain was compared to the aging A. bisporus As2796Y strain (which was previously discovered during cultivation). In the aging As2796Y mycelia, the mycelial growth rate and fruiting body yield were decreased and the chitin level and cell wall thickness were increased. Additionally, intracellular vacuoles increased, there was cytoplasmic shrinkage, and the sterol level which stabilizes the cell membrane decreased, which led to cytoplasmic outflow and the exudation of a large amount of yellow water from the mycelia. Additionally, there was increased electrolyte leakage. Furthermore, gas chromatography-mass spectrometry (GC/MS) was used to profile the metabolic changes in the aging As2796Y mycelia compared to the normal As2796 mycelia. A total of 52 differential metabolites were identified (75% were downregulated and 25% were upregulated in As2796Y). The reduction of many metabolites decreased the mycelial viability and the ability to maintain cell stability. Overall, this study is the first to report on the morphologic and metabolic changes in aged A. bisporus mycelia, which will aid future research on the mechanisms underlying A. bisporus mycelial aging.Key points• Aging of Agaricus bisporus strains will greatly reduce the fruiting body yield.• Aging of Agaricus bisporus strains can significantly change the cell structure of mycelia.• Many metabolites in the mycelium of aging spawn As2796Y significantly changed.
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http://dx.doi.org/10.1007/s00253-021-11526-1DOI Listing
October 2021

Comment on "Recent global decline of CO fertilization effects on vegetation photosynthesis".

Science 2021 09 23;373(6562):eabg5673. Epub 2021 Sep 23.

Peking University Shenzhen Graduate School, Peking University, Shenzhen 518055, Guangdong, China.

[Figure: see text].
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http://dx.doi.org/10.1126/science.abg5673DOI Listing
September 2021

Multi-component exercise training improves the physical and cognitive function of the elderly with mild cognitive impairment: a six-month randomized controlled trial.

Ann Palliat Med 2021 Aug;10(8):8919-8929

Clinical Nursing Teaching and Research Section, The Second Xiangya Hospital, Central South University, Changsha, China.

Background: This study explored the effects of multi-component exercise training on the physical and cognitive function of the elderly with mild cognitive impairment (MCI).

Methods: A total of 90 older adults with MCI were chosen from screened volunteers and randomly assigned into 2 groups (n=45), and 84 completed the 6-month follow-up. Participants in the control group received general community health instruction, while the multi-component exercise training performed on the other group. The multi-component exercise training performed on these participants was designed to suit Chinese elderly based on advice from the nursing specialist and sports medicine specialist after the preliminary experiment. The Chinese version Mini-Physical Performance Test (CM-PPT), the Mini-Mental State Examination (MMSE), and the Montreal Cognitive Assessment (MoCA) scores were examined by repeated measures analysis of variance to evaluate the physical and cognitive function of adults with MCI before and at 3 and 6 months after the intervention. The follow-up data collectors were blinded to group allocation. P<0.05 was considered statistically significant.

Results: (I) The average score of CM-PPT was increased from 11.36±2.69 to 11.88±2.40 and 12.83±2.19 in 3 and 6 months respectively after intervention, while control group was decreased from 10.79±2.73 to 10.24±2.62 in 3 months and 9.21±2.09 in 6 months. CM-PPT scores with the main intervention effect and the interaction between intervention and time were both statistically significant (P<0.05), indicating that the physical function of participants with MCI were improved after intervention. (II) The average score of MoCA was increased from 21.52±2.05 to 23.48±1.47 (3 months) and 25.19±1.29 (6 months) after intervention, while control group was decreased from 21.14.79±1.97 to 20.21±1.88 and 19.45±2.00 in 3 and 6 months. The score of MMSE showed the same trend with the score of MoCA. The MoCA score with main intervention effect and the MMSE and MoCA scores with the effect of time, the MMSE and MoCA scores with the interaction between the intervention and time were all statistically significant (P<0.05), showing that the cognitive function of participants with MCI was improved by the intervention.

Conclusions: Multi-component exercise training could significantly improve physical function and cognitive function of the elderly with MCI.

Trial Registration: Chinese Clinical Trial Registry ChiCTR2100049350.
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http://dx.doi.org/10.21037/apm-21-1809DOI Listing
August 2021

The C-terminal loop of guanosine deaminase is essential to catalysis.

Chem Commun (Camb) 2021 Sep 23;57(76):9748-9751. Epub 2021 Sep 23.

MOE Key Laboratory of Gene Function and Regulation, State Key Laboratory for Biocontrol, School of Life Sciences, Sun Yat-Sen University, Guangzhou, Guangdong, 510006, People's Republic of China.

Guanosine deaminase (GSDA) in plants specifically deaminates (de)guanosine to produce xanthosine with high specificity, which is further converted to xanthine, a key intermediate in purine metabolism and nitrogen recycling. We solved GSDA's structures from in the free and ligand-bound forms at high resolutions. Unlike GDA, the enzyme employs a single-proton shuttle mechanism for catalysis and both the substrate and enzyme undergo structural rearrangements. The last fragment of the enzyme loops back and seals the active site, and the substrate rotates during the reaction, both essential to deamination. We further identified more substrates that could be employed by the enzyme and compare it with other deaminases to reveal the recognition differences of specific substrates. Our studies provide insight into this important enzyme involved in purine metabolism and will potentially aid in the development of deaminase-based gene-editing tools.
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http://dx.doi.org/10.1039/d1cc03042fDOI Listing
September 2021

The fabrication of a highly efficient hydrogel based on a functionalized double network loaded with magnesium ion and BMP2 for bone defect synergistic treatment.

Mater Sci Eng C Mater Biol Appl 2021 Sep 30;128:112347. Epub 2021 Jul 30.

Department of Bone & Joint Surgery, Peking University Shenzhen Hospital, Shenzhen 518036, PR China; National & Local Joint Engineering Research Center of Orthopaedic Biomaterials, Peking University Shenzhen Hospital, Shenzhen 518036, PR China. Electronic address:

Although the use of bioactive ions and proteins are crucial for bone defect repair, delivering them in a stable and controlled manner remains challenging. To achieve controlled delivery of osteogenic active factor, we developed a novel double network (DN) hydrogel capable of co-delivering Mg ions and BMP2 in a controlled localized manner. This DN hydrogel was composed of poly (acrylamide) and chitosan, in which the poly (acrylamide) was cross-linked via covalent bond and the chitosan was grafted using bisphosphonate (BP) to form metal coordination bonds with Mg ions. Due to this dynamic dissociation and re-association of the "BP-Mg" coordination bond, it was possible to deliver Mg ions in a stable and controlled manner. Additionally, the obtained DN hydrogel exhibited an effective tensile strength (0.62 MPa), perfect stretchability (973% fracture strain), and good creep and recovery properties due to the dynamic cross-linking effect of "BP-Mg". Additionally, the hydrogel could synergistically promote the proliferation and differentiation of mouse embryo osteoblast precursor cells (MC3T3-E1 cells) in vitro via the BMP2/Wnt pathway. In the skull defect rat model, this positive delivery government of Mg ions and BMP2 synergistically accelerated bone regeneration. In conclusion, this dynamic cross-linked hydrogel containing Mg ions established a new platform for the sustained release of osteogenesis factor and accelerated the bone regeneration process.
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http://dx.doi.org/10.1016/j.msec.2021.112347DOI Listing
September 2021

Fatigue and dynamic biodegradation behavior of additively manufactured Mg scaffolds.

Acta Biomater 2021 Aug 29. Epub 2021 Aug 29.

National Engineering Research Center of Light Alloy Net Forming & State Key Laboratory of Metal Matrix Composite, School of Materials Science and Engineering, Shanghai Jiao Tong University, Shanghai 200240, China. Electronic address:

Additive manufacturing (AM) has enabled the fabrication of biodegradable porous metals to satisfy the desired characteristics for orthopedic applications. The geometrical design on AM biodegradable metallic scaffolds has been found to offer a favorable opportunity to regulate their mechanical and degradation performance in previous studies, however mostly confined to static responses. In this study, we presented the effect of the geometrical design on the dynamic responses of AM Mg scaffolds for the first time. Three different types of porous structures, based on various unit cells (i.e., biomimetic, diamond, and sheet-based gyroid), were established and then subjected to selective laser melting (SLM) process using group-developed Mg-Nd-Zn-Zr alloy (JDBM) powders. The topology after dynamic electropolishing, dynamic compressive properties, and dynamic biodegradation behavior of the AM Mg scaffolds were comprehensively evaluated. It was found that dynamic electropolishing effectively removed the excessive adhered powders on the surfaces and resulted in similar geometrical deviations amongst the AM Mg scaffolds, independent of their porous structures. The geometrical design significantly affected the compressive fatigue properties of the AM Mg scaffolds, of which the sheeted-based gyroid structure demonstrated a superior fatigue endurance limit of 0.85 at 10 cycles. Furthermore, in vitro dynamic immersion behaviors of the AM Mg scaffolds revealed a decent dependence on local architectures, where the sheeted-based gyroid scaffold experienced the lowest structural loss with a relatively uniform degradation mode. The obtained results indicate that the geometrical design could provide a promising strategy to develop desirable bone substitutes for the treatment of critical-size load-bearing defects. STATEMENT OF SIGNIFICANCE: Additive manufacturing (AM) has provided unprecedented opportunities to fabricate geometrically complex biodegradable scaffolds where the topological design becomes a key determinant on comprehensive performance. In this paper, we fabricate 3 AM biodegradable Mg scaffolds (i.e., biomimetic, diamond, and sheet-based gyroid) and report the effect of the geometrical design on the dynamic responses of AM Mg scaffolds for the first time. The results revealed that the sheeted-based gyroid scaffold exhibited the best combination of superior compressive fatigue properties and relatively uniform dynamic biodegradation mode, suggesting that the regulation of the porous structures could be an effective approach for the optimization of AM Mg scaffolds as to satisfy clinical requirements in orthopedic applications.
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http://dx.doi.org/10.1016/j.actbio.2021.08.040DOI Listing
August 2021

A deep learning-machine learning fusion approach for the classification of benign, malignant, and intermediate bone tumors.

Eur Radiol 2021 Aug 25. Epub 2021 Aug 25.

Department of Radiology, Nanfang Hospital, Southern Medical University, 1838 Guangzhou Avenue North, Baiyun District, Guangzhou, Guangdong Province, People's Republic of China.

Objectives: To build and validate deep learning and machine learning fusion models to classify benign, malignant, and intermediate bone tumors based on patient clinical characteristics and conventional radiographs of the lesion.

Methods: In this retrospective study, data were collected with pathologically confirmed diagnoses of bone tumors between 2012 and 2019. Deep learning and machine learning fusion models were built to classify tumors as benign, malignant, or intermediate using conventional radiographs of the lesion and potentially relevant clinical data. Five radiologists compared diagnostic performance with and without the model. Diagnostic performance was evaluated using the area under the curve (AUC).

Results: A total of 643 patients' (median age, 21 years; interquartile range, 12-38 years; 244 women) 982 radiographs were included. In the test set, the binary category classification task, the radiological model of classification for benign/not benign, malignant/nonmalignant, and intermediate/not intermediate had AUCs of 0.846, 0.827, and 0.820, respectively; the fusion models had an AUC of 0.898, 0.894, and 0.865, respectively. In the three-category classification task, the radiological model achieved a macro average AUC of 0.813, and the fusion model had a macro average AUC of 0.872. In the observation test, the mean macro average AUC of all radiologists was 0.819. With the three-category classification fusion model support, the macro AUC improved by 0.026.

Conclusion: We built, validated, and tested deep learning and machine learning models that classified bone tumors at a level comparable with that of senior radiologists. Model assistance may somewhat help radiologists' differential diagnoses of bone tumors.

Key Points: • The deep learning model can be used to classify benign, malignant, and intermediate bone tumors. • The machine learning model fusing information from radiographs and clinical characteristics can improve the classification capacity for bone tumors. • The diagnostic performance of the fusion model is comparable with that of senior radiologists and is potentially useful as a complement to radiologists in a bone tumor differential diagnosis.
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http://dx.doi.org/10.1007/s00330-021-08195-zDOI Listing
August 2021

The diagnostic value of chest X-ray in coronavirus disease 2019: A comparative study of X-ray and CT.

Sci Prog 2021 Jul-Sep;104(3):368504211016204

Radiology Department, Nanfang Hospital, Southern Medical University, Guangzhou, Guangdong, China.

As the coronavirus disease 2019 (COVID-19) epidemic spreads around the world, the demand for imaging examinations increases accordingly. The value of conventional chest radiography (CCR) remains unclear. In this study, we aimed to investigate the diagnostic value of CCR in the detection of COVID-19 through a comparative analysis of CCR and CT. This study included 49 patients with 52 CT images and chest radiographs of pathogen-confirmed COVID-19 cases and COVID-19-suspected cases that were found to be negative (non-COVID-19). The performance of CCR in detecting COVID-19 was compared to CT imaging. The major signatures that allowed for differentiation between COVID-19 and non-COVID-19 cases were also evaluated. Approximately 75% (39/52) of images had positive findings on the chest x-ray examinations, while 80.7% (42/52) had positive chest CT scans. The COVID-19 group accounted for 88.4% (23/26) of positive chest X-ray examinations and 96.1% (25/26) of positive chest CT scans. The sensitivity, specificity, and accuracy of CCR for abnormal shadows were 88%, 80%, and 87%, respectively, for all patients. For the COVID-19 group, the accuracy of CCR was 92%. The primary signature on CCR was flocculent shadows in both groups. The shadows were primarily in the bi-pulmonary, which was significantly different from non-COVID-19 patients ( = 0.008). The major CT finding of COVID-19 patients was ground-glass opacities in both lungs, while in non-COVID-19 patients, consolidations combined with ground-glass opacities were more common in one lung than both lungs ( = 0.0001). CCR showed excellent performance in detecting abnormal shadows in patients with confirmed COVID-19. However, it has limited value in differentiating COVID-19 patients from non-COVID-19 patients. Through the typical epidemiological history, laboratory examinations, and clinical symptoms, combined with the distributive characteristics of shadows, CCR may be useful to identify patients with possible COVID-19. This will allow for the rapid identification and quarantine of patients.
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http://dx.doi.org/10.1177/00368504211016204DOI Listing
September 2021

Environmental decomposition and remodeled phytotoxicity of framework-based nanomaterials.

J Hazard Mater 2021 Aug 9;422:126846. Epub 2021 Aug 9.

Key Laboratory of Pollution Processes and Environmental Criteria (Ministry of Education)/Tianjin Key Laboratory of Environmental Remediation and Pollution Control, College of Environmental Science and Engineering, Nankai University, 30080 Tianjin, China.

Zeolitic imidazole frameworks (ZIFs) have attracted a considerable amount of attention for use in environmental applications (e.g., pollutant adsorption and photocatalysis in water treatments). The environmental stability and toxicity of ZIFs are key prerequisites for their practical applications, but information about these factors is largely lacking. The present work finds that pristine ZIFs (ZIF-8 and ZIF-67) photodegrade from frame structures into two-dimensional nanosheets and are oxidized to zinc carbonate (ZIF-8) and CoO (ZIF-67) under visible-light irradiation. The photoinduced electrons, holes and free radicals promote dissolution of the metal cores and organic ligands, leading to collapse of the frame structure. The photodegradation of ZIF-8 alleviates developmental inhibition, oxidative stress, plasmolysis, and photosynthetic toxicity, while the photodegradation of ZIF-67 aggravates nanotoxicity. The integration of metabolomics and transcriptomics analysis reveals that unsaturated fatty acid biosynthesis and metal ion-binding transcription contribute to the altered toxicity of ZIF photodegradation. These findings highlight the roles of photodegradation in structural transformation and alteration of the toxicity of ZIFs, alarming the study of pristine metal-organic frameworks (MOFs).
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http://dx.doi.org/10.1016/j.jhazmat.2021.126846DOI Listing
August 2021

Regulatory effects of Lactobacillus fermented black barley on intestinal microbiota of NAFLD rats.

Food Res Int 2021 09 10;147:110467. Epub 2021 Jun 10.

Department of Food Science and Engineer, School of Agriculture and Biology, Shanghai Jiao Tong University, Shanghai 200240, China. Electronic address:

Gut microbiota dysbiosis and oxidative stress may play important roles in the progression of nonalcoholic fatty liver disease (NAFLD). Fermented foods contain probiotics and other bioactive components that may improve gastrointestinal health and provide other health benefits. Here, we investigated the effect of Lactobacillus-fermented black barley on NAFLD rats. Adult Sprague Dawley rats were randomized into four groups: normal chow diet (NC), high-fat diet (HF), HF + fermented black barley treatment (HB) and HF + Lactobacillus treatment (HL). The rats in the HB and HL groups were continuously administered fermented black barley or Lactobacillus, respectively, for 12 weeks (1 mL/100 g·BW, containing 1 × 10 CFU/mL Lactobacillus). Compared with the HF treatment, HB treatment effectively inhibited the increase in body weight, liver and abdominal fat indexes and hepatic lipids (p < 0.01), increased hepatic SOD activity (p < 0.05), decreased thiobarbituric acid reactive substances (TBARSs) (p < 0.01) and improved liver function. Moreover, Lactobacillus-fermented black barley exhibited regulatory effect on high-fat diet-induced intestinal microbiota dysbiosis by increasing the relative microbiota abundance and diversity, increasing the relative abundance of Bacteroidetes, decreasing the Firmicutes/Bacteroidetes ratio, increasing the abundances of some intestinal probiotics (such as Akkermansia and Lactococcus), and influencing some of the fecal metabolites related to hormones and lipid metabolism. The supplementation of fermented cereal food might be a new effective and safe preventive dietary strategy against NAFLD.
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http://dx.doi.org/10.1016/j.foodres.2021.110467DOI Listing
September 2021

The Crystal Structure of the Chemosensory Protein CSP8.

Insects 2021 Jul 1;12(7). Epub 2021 Jul 1.

MOE Key Laboratory of Gene Function and Regulation, State Key Laboratory for Biocontrol, School of Life Sciences, Sun Yat-Sen University, Guangzhou 510006, China.

F. is a generalist herbivore and one of the most important economic pests feeding on about 300 host plants in many Asian countries. Specific insect behaviors can be stimulated after recognizing chemicals in the external environment through conserved chemosensory proteins (CSPs) in chemoreceptive organs, which are critical components of the olfactory systems. To explore its structural basis for ligand-recognizing capability, we solved the 2.3 Å crystal structure of the apoprotein of CSP8 (SlCSP8). The SlCSP8 protein displays a conserved spherical shape with a negatively charged surface. Our binding assays showed that SlCSP8 bound several candidate ligands with differential affinities, with rhodojaponin III being the most tightly bound ligand. Our crystallographic and biochemical studies provide important insight into the molecular recognition mechanism of the sensory protein SlCSP8 and the CSP family in general, and they suggest that CSP8 is critical for insects to identify rhodojaponin III, which may aid in the CSP-based rational drug design in the future.
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http://dx.doi.org/10.3390/insects12070602DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8305471PMC
July 2021

Can the delayed phase of quantitative contrast-enhanced mammography improve the diagnostic performance on breast masses?

Quant Imaging Med Surg 2021 Aug;11(8):3684-3697

Department of Ultrasonic Medicine, Nanfang Hospital, Southern Medical University, Guangzhou, China.

Background: Contrast-enhanced mammography (CEM) is an imaging tool for breast cancer detection. Most quantitative analyses of CEM involve two phases, and it is unknown whether an added delayed phase can improve its diagnostic performance compared to dynamic contrast-enhanced magnetic resonance imaging (DCE-MRI). This study aimed to evaluate whether the delayed phase improves the diagnostic performance of CEM in distinguishing malignant and benign masses.

Methods: This prospective study enrolled 111 women with 111 pathologically confirmed breast masses. CEM was performed after the injection of contrast agent between 2-3 minutes (T1, early phase), 4-5 minutes (T2, second phase), and 7-9 minutes (T3, delayed phase). The quantitative enhanced gray value of lesions (LGV) and the lesion to background grey value ratio (LBR) were measured within each phase's corresponding region of interest (ROI). Based on their changes, the kinetic enhancement pattern was assessed among the three phases, and the diagnostic performance was subsequently measured.

Results: The LGV and LBR of malignant masses were significantly greater than those of benign lesions. The diagnostic performance of LGV and LBR at the delayed phase was consistent with that of the second phase but poorer than that of the early phase. The sensitivity of LGV + LGV + LGV was less than that of LGV + LGV (86.5% 95.1%) with a similar area under the curve (AUC), specificity, positive-predictive value (PPV), negative-predictive value (NPV), and accuracy. The sensitivity of LBR + LBR + LBR increased by 19.6%, and specificity decreased by 20.7% compared with LBR + LBR. The LGV + LGV + LGV + kinetic enhancement (T1-T3) had the lowest sensitivity (67.0%), but the highest specificity (75.8%), and the sensitivity of LBR + LBR + LBR + kinetic enhancement (T1-T3) was higher than that of LBR + LBR + kinetic enhancement (T1-T2) (90.2% 63.4%, respectively).

Conclusions: The addition of a delayed CEM phase for breast cancer diagnosis yielded limited performance improvement. The quantitative analysis combined with enhancement patterns between the two consecutive phases has great potential to distinguish between malignant and benign lesions.
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http://dx.doi.org/10.21037/qims-20-1092DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8245946PMC
August 2021

Human Hyaluronidase PH20 Potentiates the Antitumor Activities of Mesothelin-Specific CAR-T Cells Against Gastric Cancer.

Front Immunol 2021 13;12:660488. Epub 2021 Jul 13.

Center for Cell Regeneration and Biotherapy, Guangzhou Institutes of Biomedicine and Health, Chinese Academy of Sciences, Guangzhou, China.

T cell infiltration into tumors is essential for successful immunotherapy against solid tumors. Herein, we found that the expression of hyaluronic acid synthases (HAS) was negatively correlated with patient survival in multiple types of solid tumors including gastric cancer. HA impeded anti-tumor activities of anti-mesothelin (MSLN) chimeric antigen receptor T cells (CAR-T cells) against gastric cancer cells by restricting CAR-T cell mobility . We then constructed a secreted form of the human hyaluronidase PH20 (termed sPH20-IgG2) by replacing the PH20 signal peptide with a tPA signal peptide and attached with IgG2 Fc fragments. We found that overexpression of sPH20-IgG2 promoted CAR-T cell transmigration through an HA-containing matrix but did not affect the cytotoxicity or cytokine secretion of the CAR-T cells. In BGC823 and MKN28 gastric cancer cell xenografts, sPH20-IgG2 promoted anti-mesothelin CAR-T cell infiltration into tumors. Furthermore, mice infused with sPH20-IgG2 overexpressing anti-MSLN CAR-T cells had smaller tumors than mice injected with anti-MSLN CAR-T cells. Thus, we demonstrated that sPH20-IgG2 can enhance the antitumor activity of CAR-T cells against solid tumors by promoting CAR-T cell infiltration.
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http://dx.doi.org/10.3389/fimmu.2021.660488DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8313856PMC
October 2021

Achieved blood pressure post-acute kidney injury and risk of adverse outcomes after AKI: A prospective parallel cohort study.

BMC Nephrol 2021 Jul 29;22(1):270. Epub 2021 Jul 29.

Division of Nephrology, University of California, San Francisco School of Medicine, San Francisco, CA, USA.

Background: There has recently been considerable interest in better understanding how blood pressure should be managed after an episode of hospitalized AKI, but there are scant data regarding the associations between blood pressure measured after AKI and subsequent adverse outcomes. We hypothesized that among AKI survivors, higher blood pressure measured three months after hospital discharge would be associated with worse outcomes. We also hypothesized these associations between blood pressure and outcomes would be similar among those who survived non-AKI hospitalizations.

Methods: We quantified how systolic blood pressure (SBP) observed three months after hospital discharge was associated with risks of subsequent hospitalized AKI, loss of kidney function, mortality, and heart failure events among 769 patients in the prospective ASSESS-AKI cohort study who had hospitalized AKI. We repeated this analysis among the 769 matched non-AKI ASSESS-AKI enrollees. We then formally tested for AKI interaction in the full cohort of 1538 patients to determine if these associations differed among those who did and did not experience AKI during the index hospitalization.

Results: Among 769 patients with AKI, 42 % had subsequent AKI, 13 % had loss of kidney function, 27 % died, and 18 % had heart failure events. SBP 3 months post-hospitalization did not have a stepwise association with the risk of subsequent AKI, loss of kidney function, mortality, or heart failure events. Among the 769 without AKI, there was also no stepwise association with these risks. In formal interaction testing using the full cohort of 1538 patients, hospitalized AKI did not modify the association between post-discharge SBP and subsequent risks of adverse clinical outcomes.

Conclusions: Contrary to our first hypothesis, we did not observe that higher stepwise blood pressure measured three months after hospital discharge with AKI was associated with worse outcomes. Our data were consistent with our second hypothesis that the association between blood pressure measured three months after hospital discharge and outcomes among AKI survivors is similar to that observed among those who survived non-AKI hospitalizations.
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http://dx.doi.org/10.1186/s12882-021-02480-1DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8320241PMC
July 2021

Updated description of (Mycenaceae, Agaricales), including three new species with brightly coloured pilei from Yunnan Province, southwest China.

MycoKeys 2021 14;81:139-164. Epub 2021 Jul 14.

Shandong Key Laboratory of Edible Mushroom Technology, School of Agriculture, Ludong University, Yantai 264025, China Ludong University Yantai China.

An updated description of the genus , combining macro- and micromorphological characters that elaborate on the original generic characterisation, is presented. is characterised by a brightly coloured pileus, all tissues inamyloid and pileipellis covered with simple to branched excrescences. Previously, nine species were known globally, of which three species were accepted in China. Three newly-recognised species classified in the genus are here formally described from Yunnan Province: , and The new species are characterised by a yellow, orange, pink or red pileus, fusiform cheilocystidia and pleurocystidia, non-smooth pileipellis, stipitipellis smooth or with cylindrical ornamentation, caulocystidia fusiform or subglobose, if present and all tissues inamyloid. Morphological descriptions, photographs, line drawings and comparisons with closely-related taxa are presented for the new species. A phylogenetic analysis of sequence data for the rDNA internal transcribed spacer region and nuclear large ribosomal subunit (ITS + nLSU) supported that is resolved as monophyletic and also supported the taxonomic recognition of the new species. A key to the 12 species of is also provided.
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http://dx.doi.org/10.3897/mycokeys.81.67773DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8295246PMC
July 2021

Allosteric drug transport mechanism of multidrug transporter AcrB.

Nat Commun 2021 06 29;12(1):3889. Epub 2021 Jun 29.

Institute of Biochemistry, Goethe-University Frankfurt, Frankfurt am Main, Germany.

Gram-negative bacteria maintain an intrinsic resistance mechanism against entry of noxious compounds by utilizing highly efficient efflux pumps. The E. coli AcrAB-TolC drug efflux pump contains the inner membrane H/drug antiporter AcrB comprising three functionally interdependent protomers, cycling consecutively through the loose (L), tight (T) and open (O) state during cooperative catalysis. Here, we present 13 X-ray structures of AcrB in intermediate states of the transport cycle. Structure-based mutational analysis combined with drug susceptibility assays indicate that drugs are guided through dedicated transport channels toward the drug binding pockets. A co-structure obtained in the combined presence of erythromycin, linezolid, oxacillin and fusidic acid shows binding of fusidic acid deeply inside the T protomer transmembrane domain. Thiol cross-link substrate protection assays indicate that this transmembrane domain-binding site can also accommodate oxacillin or novobiocin but not erythromycin or linezolid. AcrB-mediated drug transport is suggested to be allosterically modulated in presence of multiple drugs.
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http://dx.doi.org/10.1038/s41467-021-24151-3DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8242077PMC
June 2021

Human ACE2-Functionalized Gold "Virus-Trap" Nanostructures for Accurate Capture of SARS-CoV-2 and Single-Virus SERS Detection.

Nanomicro Lett 2021 Apr 13;13(1):109. Epub 2021 Apr 13.

State Key Laboratory of Oncogenes and Related Genes, Shanghai Cancer Institute, Renji Hospital, Shanghai Jiaotong University School of Medicine, Shanghai, 200032, People's Republic of China.

The current COVID-19 pandemic urges the extremely sensitive and prompt detection of SARS-CoV-2 virus. Here, we present a Human Angiotensin-converting-enzyme 2 (ACE2)-functionalized gold "virus traps" nanostructure as an extremely sensitive SERS biosensor, to selectively capture and rapidly detect S-protein expressed coronavirus, such as the current SARS-CoV-2 in the contaminated water, down to the single-virus level. Such a SERS sensor features extraordinary 10-fold virus enrichment originating from high-affinity of ACE2 with S protein as well as "virus-traps" composed of oblique gold nanoneedles, and 10-fold enhancement of Raman signals originating from multi-component SERS effects. Furthermore, the identification standard of virus signals is established by machine-learning and identification techniques, resulting in an especially low detection limit of 80 copies mL for the simulated contaminated water by SARS-CoV-2 virus with complex circumstance as short as 5 min, which is of great significance for achieving real-time monitoring and early warning of coronavirus. Moreover, here-developed method can be used to establish the identification standard for future unknown coronavirus, and immediately enable extremely sensitive and rapid detection of novel virus.
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http://dx.doi.org/10.1007/s40820-021-00620-8DOI Listing
April 2021

Associations between female lung cancer risk and sex steroid hormones: a systematic review and meta-analysis of the worldwide epidemiological evidence on endogenous and exogenous sex steroid hormones.

BMC Cancer 2021 Jun 10;21(1):690. Epub 2021 Jun 10.

Department of Thoracic Surgery, National Cancer Center/National Clinical Research Center for Cancer/Cancer Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, 17 Panjiayuan Nanli, Chaoyang District, Beijing, 100021, China.

Background: Published findings suggest sex differences in lung cancer risk and a potential role for sex steroid hormones. Our aim was to perform a meta-analysis to investigate the effects of sex steroid hormone exposure specifically on the risk of lung cancer in women.

Methods: The PubMed, MEDLINE, Web of Science, and EMBASE databases were searched. The pooled odds ratios (ORs) and 95% confidence intervals (95% CIs) for female lung cancer risk associated with sex steroid hormones were calculated overall and by study design, publication year, population, and smoking status. Sensitivity analysis, publication bias, and subgroup analysis were performed.

Results: Forty-eight studies published between 1987 and 2019 were included in the study with a total of 31,592 female lung cancer cases and 1,416,320 subjects without lung cancer. Overall, higher levels of sex steroid hormones, both endogenous (OR: 0.92, 95% CI: 0.87-0.98) and exogenous (OR: 0.86, 95% CI: 0.80-0.93), significantly decreased the risk of female lung cancer by 10% (OR: 0.90, 95% CI: 0.86-0.95). The risk of lung cancer decreased more significantly with a higher level of sex steroid hormones in non-smoking women (OR: 0.88, 95% CI: 0.78-0.99) than in smoking women (OR: 0.98, 95% CI: 0.77-1.03), especially in Asia women (OR: 0.84, 95% CI: 0.74-0.96).

Conclusions: Our meta-analysis reveals an association between higher levels of sex steroid hormone exposure and the decreased risk of female lung cancer. Surveillance of sex steroid hormones might be used for identifying populations at high risk for lung cancer, especially among non-smoking women.
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http://dx.doi.org/10.1186/s12885-021-08437-9DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8194027PMC
June 2021

Low-intensity pulsed ultrasound promotes osteoarthritic cartilage regeneration by BMSC-derived exosomes via modulating the NF-κB signaling pathway.

Int Immunopharmacol 2021 Aug 5;97:107824. Epub 2021 Jun 5.

Department of Rehabilitation Medicine, Shunde Hospital of Southern Medical University, Foshan 528000, China; Department of Rehabilitation Medicine, Nanfang Hospital of Southern Medical University, Guangzhou 510000, China. Electronic address:

Osteoarthritis is the most common disabling joint disease throughout the world, and the effect of therapy on its course is still unsatisfactory in clinical practice. Recent studies have shown that mesenchymal stem cell (MSC)-derived exosomes can promote cartilage repair and regeneration in osteoarthritis, indicating that these exosomes could be a novel and promising strategy for treating osteoarthritis. This study investigated whether low-intensity pulsed ultrasound (LIPUS) enhances the effects of bone marrow MSC (BMSC)-derived exosomes on cartilage regeneration in osteoarthritis and examined the underlying mechanism. Our results revealed that BMSC-derived exosomes display the typical morphological features of exosomes. LIPUS-mediated BMSC-derived exosomes promoted cartilage regeneration, increased chondrocyte proliferation and extracellular matrix synthesis, suppressed inflammation, and inhibited the interleukin (IL)-1β-induced activation of the nuclear factor kappa B (NF-κB) pathway. In brief, LIPUS enhances the promoting effects of BMSC-derived exosomes on osteoarthritic cartilage regeneration, mainly by strengthening the inhibition of inflammation and further enhancing chondrocyte proliferation and cartilage matrix synthesis. The underlying mechanism could be related to the inhibition of the IL-1β-induced activation of the NF-κB pathway.
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http://dx.doi.org/10.1016/j.intimp.2021.107824DOI Listing
August 2021

Distinct durability of IgM/IgG antibody responses in COVID-19 patients with differing severity.

Sci China Life Sci 2021 Jun 2. Epub 2021 Jun 2.

CAS Key Laboratory of Pathogenic Microbiology and Immunology, Institute of Microbiology, Center for Influenza Research and Early-warning (CASCIRE), CAS-TWAS Center of Excellence for Emerging Infectious Diseases (CEEID), Chinese Academy of Sciences, Beijing, 100101, China.

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http://dx.doi.org/10.1007/s11427-020-1947-yDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8176871PMC
June 2021

Remodeling of the tumor microenvironment using an engineered oncolytic vaccinia virus improves PD-L1 inhibition outcomes.

Biosci Rep 2021 Jun;41(6)

Molecular Diagnosis Laboratory, Zhejiang Provincial People's Hospital, People's Hospital of Hangzhou Medical College, Hangzhou, China.

Immune checkpoint inhibitor (ICI) immunotherapies have vastly improved therapeutic outcomes for patients with certain cancer types, but these responses only manifest in a small percentage of all cancer patients. The goal of the present study was to improve checkpoint therapy efficacy by utilizing an engineered vaccinia virus to improve the trafficking of lymphocytes to the tumor, given that such lymphocyte trafficking is positively correlated with patient checkpoint inhibitor response rates. We developed an oncolytic vaccinia virus (OVV) platform expressing manganese superoxide dismutase (MnSOD) for use as both a monotherapy and together with anti-PD-L1. Intratumoral OVV-MnSOD injection in immunocompetent mice resulted in inflammation within poorly immunogenic tumors, thereby facilitating marked tumor regression. OVV-MnSOD administration together with anti-PD-L1 further improved antitumor therapy outcomes in models in which these monotherapy approaches were ineffective. Overall, our results emphasize the value of further studying these therapeutic approaches in patients with minimally or non-inflammatory tumors.
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http://dx.doi.org/10.1042/BSR20204186DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8193643PMC
June 2021

Identification of A-to-I RNA editing profiles and their clinical relevance in lung adenocarcinoma.

Sci China Life Sci 2021 May 27. Epub 2021 May 27.

Department of Epidemiology, Center for Global Health, School of Public Health, Nanjing Medical University, Nanjing, 211166, China.

Adenosine-to-inosine (A-to-I) RNA editing is a widespread posttranscriptional modification that has been shown to play an important role in tumorigenesis. Here, we evaluated a total of 19,316 RNA editing sites in the tissues of 80 lung adenocarcinoma (LUAD) patients from our Nanjing Lung Cancer Cohort (NJLCC) and 486 LUAD patients from the TCGA database. The global RNA editing level was significantly increased in tumor tissues and was highly heterogeneous across patients. The high RNA editing level in tumors was attributed to both RNA (ADAR1 expression) and DNA alterations (mutation load). Consensus clustering on RNA editing sites revealed a new molecular subtype (EC3) that was associated with the poorest prognosis of LUAD patients. Importantly, the new classification was independent of classic molecular subtypes based on gene expression or DNA methylation. We further proposed a simplified model including eight RNA editing sites to accurately distinguish the EC3 subtype in our patients. The model was further validated in the TCGA dataset and had an area under the curve (AUC) of the receiver operating characteristic curve of 0.93 (95%CI: 0.91-0.95). In addition, we found that LUAD cell lines with the EC3 subtype were sensitive to four chemotherapy drugs. These findings highlighted the importance of RNA editing events in the tumorigenesis of LUAD and provided insight into the application of RNA editing in the molecular subtyping and clinical treatment of cancer.
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http://dx.doi.org/10.1007/s11427-020-1928-0DOI Listing
May 2021

Deep Insight Into Long Non-coding RNA and mRNA Transcriptome Profiling in HepG2 Cells Expressing Genotype IV Swine Hepatitis E Virus ORF3.

Front Vet Sci 2021 29;8:625609. Epub 2021 Apr 29.

College of Veterinary Medicine, Southwest University, Chongqing, China.

Swine hepatitis E (swine HE) is a new type of zoonotic infectious disease caused by the swine hepatitis E virus (swine HEV). Open reading frame 3 (ORF3) is an important virulent protein of swine HEV, but its function still is mainly unclear. In this study, we generated adenoviruses ADV4-ORF3 and ADV4 negative control (ADV4-NC), which successfully mediated overexpression of enhanced green fluorescent protein (EGFP)-ORF3 and EGFP, respectively, in HepG2 cells. High-throughput sequencing was used to screen for differentially expressed long non-coding RNAs (lncRNAs) and messenger RNAs (mRNAs). The -target genes of lncRNAs were predicted, functional enrichment (Gene Ontology [GO] and Kyoto Encyclopedia of Genes and Genomes [KEGG]) was performed, and 12 lncRNAs with statistically significant different expressions ( ≤ 0.05 and ≤ 1) were selected for further quantitative real-time reverse transcription (qRT-PCR) validation. In HepG2 cells, we identified 62 significantly differentially expressed genes (DEGs) (6,564 transcripts) and 319 lncRNAs (124 known lncRNAs and 195 novel lncRNAs) that were affected by ORF3, which were involved in systemic lupus erythematosus, infection, signaling pathways pluripotency regulation of stem cells, the peroxisome proliferator-activated receptor (PPAR) signaling pathway, and platinum drug resistance pathways. -target gene prediction identified 45 lncRNAs corresponding to candidate mRNAs, among which eight were validated by qRT-PCR: (two transcripts), , and (3 transcripts). Our results revealed that the lncRNA profile in host cells affected by ORF3, swine HEV ORF3, might affect the pentose and glucuronate interconversions and mediate the formation of obstructive jaundice by influencing bile secretion, which will help to determine the function of ORF3 and the infection mechanism and treatment of swine HE.
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http://dx.doi.org/10.3389/fvets.2021.625609DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8116512PMC
April 2021

First record of the rare genus (Psathyrellaceae, Agaricales) from China with description of two new species.

MycoKeys 2021 23;79:119-128. Epub 2021 Apr 23.

Jiangxi Key Laboratory for Conservation and Utilization of Fungal Resource; Key Laboratory of State Forestry Administration on Forest Ecosystem Protection and Restoration of Poyang Lake Watershed, Jiangxi Agricultural University, Nanchang, Jiangxi 330045, China Jiangxi Agricultural University Nanchang China.

is a rare genus that comprises two species and that has previously been reported only from Europe and North America. The present study expands the geographical scope of the genus by describing two new species - and - from subtropical China. The new species are supported by morphological characteristics and phylogenetic analyses (ITS, LSU and ). The new species have very similar morphological characteristics and are 98% similar in their ITS region. However, has two types of long gills at the same time, rarely fusiform pleurocystidia with rostrum. Detailed descriptions, colour photos, illustrations and a key to related species are presented in this paper.
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http://dx.doi.org/10.3897/mycokeys.79.63700DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8087613PMC
April 2021

Early and consecutive RT-PCR tests with both oropharyngeal swabs and sputum could improve testing yield for patients with COVID-19: An observation cohort study in China.

Int J Infect Dis 2021 Jun 28;107:242-246. Epub 2021 Apr 28.

Beijing Ditan Hospital, Capital Medical University, Beijing 100102, China; Beijing Key Laboratory of Emerging Infectious Diseases, Beijing 100102, China. Electronic address:

Objective: The real-time polymerase chain reaction (RT-PCR) test is recommended for the diagnosis of COVID-19 and provides a powerful tool to identify new infections and facilitate contact tracing. In fact, as the prevalence of COVID-19 decreases, this RT-PCR testing remains as the main preventive measure to avoid rebound. However, inconsistent results can lead to misdiagnoses in the clinic. These inconsistencies are due to the variability in (1) the collection times of biological samples post infection, and (2) sampling procedures.

Methods: We applied the Kaplan-Meier method and multivariate logistic regression on RT-PCR results from 258 confirmed patients with COVID-19 to evaluate the factors associated with negative conversion. We also estimated the proportion (%) of negative conversion among patients who had tested twice or more, and compared the proportions arising from oropharyngeal swabs, sputum, and combined double testing, respectively.

Main Results: The proportion of negative conversion was 6.7% on day 4, 16.4% on day 7, 41.0% at 2 weeks, and 61.0% at 3 weeks post-admission. We also found that 34.1% and 60.3% of subjects had at least one negative RT-PCR result on days 7 and 14 after the onset of symptoms, respectively. The proportion of negative conversions following sputum testing was higher than that from oropharyngeal swabs in the early stages but this declined after the onset of symptoms.

Conclusion: In the absence of effective treatments or vaccines, efficient testing strategies are critical if we are to control the COVID-19 epidemic. According to this study, early, consecutive and combined double testing, will be the key to identify infected patients, particularly for asymptomatic and mild symptomatic cases. These strategies will minimize misdiagnosis and the ineffective isolation of infected patients.
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http://dx.doi.org/10.1016/j.ijid.2021.04.076DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8079261PMC
June 2021

Sequential Superassembly of Nanofiber Arrays to Carbonaceous Ordered Mesoporous Nanowires and Their Heterostructure Membranes for Osmotic Energy Conversion.

J Am Chem Soc 2021 05 30;143(18):6922-6932. Epub 2021 Apr 30.

Department of Chemistry, Shanghai Key Lab of Molecular Catalysis and Innovative Materials, Collaborative Innovation Center of Chemistry for Energy Materials, Fudan University, Shanghai 200438, P. R. China.

The capture of sustainable energy from a salinity gradient, in particular, using renewable biomass-derived functional materials, has attracted significant attention. In order to convert osmotic energy to electricity, many membrane materials with nanofluidic channels have been developed. However, the high cost, complex preparation process, and low output power density still restrict the practical application of traditional membranes. Herein, we report the synthesis of highly flexible and mechanically robust nanofiber-arrays-based carbonaceous ordered mesoporous nanowires (CMWs) through a simple and straightforward soft-templating hydrothermal carbonization approach. This sequential superassembly strategy shows a high yield and great versatility in controlling the dimensions of CMWs with the aspect ratio changes from about 3 to 39. Furthermore, these CMWs can be used as novel building blocks to construct functional hybrid membranes on macroporous alumina. This nanofluidic membrane with asymmetric geometry and charge polarity exhibits low resistance and high-performance energy conversion. This work opens a solution-based route for the one-pot preparation of CMWs and functional heterostructure membranes for various applications.
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http://dx.doi.org/10.1021/jacs.1c00547DOI Listing
May 2021

The Mechanism of Facultative Intracellular Parasitism of .

Int J Mol Sci 2021 Apr 1;22(7). Epub 2021 Apr 1.

College of Veterinary Medicine, Southwest University, Chongqing 402460, China.

Brucellosis is a highly prevalent zoonotic disease characterized by abortion and reproductive dysfunction in pregnant animals. Although the mortality rate of Brucellosis is low, it is harmful to human health, and also seriously affects the development of animal husbandry, tourism and international trade. Brucellosis is caused by , which is a facultative intracellular parasitic bacteria. It mainly forms -containing vacuoles (BCV) in the host cell to avoid the combination with lysosome (Lys), so as to avoid the elimination of it by the host immune system. not only has the ability to resist the phagocytic bactericidal effect, but also can make the host cells form a microenvironment which is conducive to its survival, reproduction and replication, and survive in the host cells for a long time, which eventually leads to the formation of chronic persistent infection. can proliferate and replicate in cells, evade host immune response and induce persistent infection, which are difficult problems in the treatment and prevention of Brucellosis. Therefore, the paper provides a preliminary overview of the facultative intracellular parasitic and immune escape mechanisms of , which provides a theoretical basis for the later study on the pathogenesis of .
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http://dx.doi.org/10.3390/ijms22073673DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8036852PMC
April 2021

Downregulation of SELENBP1 enhances oral squamous cell carcinoma chemoresistance through KEAP1-NRF2 signaling.

Cancer Chemother Pharmacol 2021 08 27;88(2):223-233. Epub 2021 Apr 27.

School of Stomatology, Xi'an Medical University, Xi'an, Shaanxi, 710021, China.

Purpose: Limited value is achieved in systemic chemotherapy for oral squamous cell carcinoma (OSCC), due to cancer cell resistance against cytotoxic agents. Tumor suppressor activities of selenium-binding protein 1 (SELENBP1) have been shown in multiple human cancers except for OSCC. The aim of this study is to clarify the biological functions and potential mechanism of SELENBP1 in OSCC.

Methods: SELENBP1 expression and its clinical significance in OSCC were analyzed from The Cancer Genome Atlas (TCGA) database. Quantitative polymerase chain reaction (qPCR) or western blot was applied to determine SELENBP1, NRF2 and KEAP1 mRNA or protein levels. Sulforhodamine B assay (SRB) was performed to examine the cytotoxic effects of 5-fluorouracil (5-FU) and cisplatin on OSCC cells. Luciferase reporter assay and chromatin immunoprecipitation (ChIP) assay were conducted to investigate the role of SELENBP1 in KEAP1 transcription.

Results: SELENBP1 downregulation is positively correlated with a poor prognosis for OSCC patients. SELENBP1 knockdown enhances resistance of OSCC cells to 5-FU and cisplatin, while SENENBP1 overexpression displays the opposite effects. Mechanistically, SELENBP1 reduces NRF2 protein levels by promoting its polyubiquitination and degradation. SELENBP1 induces KEAP1 transcription by binding to KEAP1 promoter. Downregulation of SELENBP1 is induced by miR-4786-3p binding to the 3' untranslated region (UTR) of SELENBP1.

Conclusion: SENENBP1 is identified as a novel protective biomarker for OSCC patients. Targeting at the miR-4786-3p-SELENBP1-KEAP1-NRF2 signaling axis may enhance the efficacy of chemotherapy for OSCC.
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http://dx.doi.org/10.1007/s00280-021-04284-4DOI Listing
August 2021

lncRNA PCAT6 facilitates cell proliferation and invasion via regulating the miR-326/hnRNPA2B1 axis in liver cancer.

Oncol Lett 2021 Jun 13;21(6):471. Epub 2021 Apr 13.

Department of Intervention, The Cancer Hospital of the University of Chinese Academy of Sciences, Zhejiang Cancer Hospital, Institute of Basic Medicine and Cancer, Chinese Academy of Sciences, Hangzhou, Zhejiang 310022, P.R. China.

Liver cancer is one of the most common malignant human tumors with the highest morbidity and mortality rates of all cancer types in China. Evidence suggests that long non-coding RNA prostate cancer-associated transcript 6 (PCAT6) plays an essential role in tumor progression. However, the roles and mechanism of PCAT6 in liver cancer remain unclear. The present study showed that the expression of PCAT6 and heterogeneous nuclear ribonucleoprotein A2B1 (hnRNPA2B1) was upregulated in liver cancer tissues compared with non-cancerous tissues and were associated with poor overall survival time, whereas microRNA (miR)-326 expression was downregulated. Moreover, knockdown of PCAT6 significantly inhibited the proliferation and invasion of liver cancer cells and . A dual-luciferase reporter gene assay demonstrated that PCAT6 could bind to miR-326 and that hnRNPA2B1 was a direct target gene of miR-326. Mechanistically, silenced PCAT6 suppressed the malignant phenotype of liver cancer cells through upregulating the inhibitory effect of miR-326 on hnRNPA2B1 expression. Taken together, these data demonstrated that knockdown of PCAT6 inhibited liver cancer progression through regulation of the miR-326/hnRNPA2B1 axis, suggesting that PCAT6 functions as an oncogene and may be a useful biomarker for the future diagnosis and treatment of liver cancer.
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http://dx.doi.org/10.3892/ol.2021.12732DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8063269PMC
June 2021
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