Publications by authors named "Hui Yu"

1,249 Publications

  • Page 1 of 1

VEGF121 Mediates Post-Hypoxia Cardioprotective Effects Via CaSR and Mitochondria-Dependent Protease Pathway.

Arq Bras Cardiol 2021 Sep;117(3):476-483

The First People's Hospital of Guiyang, Guiyang - China.

Background: Cardiovascular disease is the major cause of death worldwide. Hypoxia-mediated apoptosis in cardiomyocytes is a major cause of cardiovascular disorders. Treatment with vascular endothelial growth factor (VEGF) protein has been tested but operational difficulties have limited its use. However, with the advancements of gene therapy, interest has risen in VEGF-based gene therapy in cardiovascular disorders. However, the precise mechanism by which VEGF replenishment rescues post-hypoxia damage in cardiomyocytes is not known.

Objectives: To investigate the effect of post-hypoxia VEGF121 expression using neonatal rat cardiomyocytes.

Methods: Cardiomyocytes isolated from neonatal rats were used to establish an in vitro model of hypoxia-induced cardiac injury. The effect of VEGF overexpression, alone or in combination with small-molecule inhibitors targeting calcium channel, calcium sensitive receptors (CaSR), and calpain on cell growth and proliferation on hypoxia-induced cardiomyocyte injury were determined using an MTT assay, TUNEL staining, Annexin V/PI staining, lactate dehydrogenase and caspase activity. For statistical analysis, a value of P<0.05 was considered to be significant.

Results: The effect of VEGF121 was found to be mediated by CaSR and calpain but was not dependent on calcium channels.

Conclusions: Our findings, even though using an in vitro setting, lay the foundation for future validation and pre-clinical testing of VEGF-based gene therapy in cardiovascular diseases.
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http://dx.doi.org/10.36660/abc.20190902DOI Listing
September 2021

DR-MIL: deep represented multiple instance learning distinguishes COVID-19 from community-acquired pneumonia in CT images.

Comput Methods Programs Biomed 2021 Sep 9;211:106406. Epub 2021 Sep 9.

Department of Radiology, The Affiliated Hospital of Guizhou Medical University, Guiyang, China. Electronic address:

Background And Objective: Given that the novel coronavirus disease 2019 (COVID-19) has become a pandemic, a method to accurately distinguish COVID-19 from community-acquired pneumonia (CAP) is urgently needed. However, the spatial uncertainty and morphological diversity of COVID-19 lesions in the lungs, and subtle differences with respect to CAP, make differential diagnosis non-trivial.

Methods: We propose a deep represented multiple instance learning (DR-MIL) method to fulfill this task. A 3D volumetric CT scan of one patient is treated as one bag and ten CT slices are selected as the initial instances. For each instance, deep features are extracted from the pre-trained ResNet-50 with fine-tuning and represented as one deep represented instance score (DRIS). Each bag with a DRIS for each initial instance is then input into a citation k-nearest neighbor search to generate the final prediction. A total of 141 COVID-19 and 100 CAP CT scans were used. The performance of DR-MIL is compared with other potential strategies and state-of-the-art models.

Results: DR-MIL displayed an accuracy of 95% and an area under curve of 0.943, which were superior to those observed for comparable methods. COVID-19 and CAP exhibited significant differences in both the DRIS and the spatial pattern of lesions (p<0.001). As a means of content-based image retrieval, DR-MIL can identify images used as key instances, references, and citers for visual interpretation.

Conclusions: DR-MIL can effectively represent the deep characteristics of COVID-19 lesions in CT images and accurately distinguish COVID-19 from CAP in a weakly supervised manner. The resulting DRIS is a useful supplement to visual interpretation of the spatial pattern of lesions when screening for COVID-19.
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http://dx.doi.org/10.1016/j.cmpb.2021.106406DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8426140PMC
September 2021

PM promotes Drp1-mediated mitophagy to induce hepatic stellate cell activation and hepatic fibrosis via regulating miR-411.

Exp Cell Res 2021 Sep 8;407(2):112828. Epub 2021 Sep 8.

Department of Pediatrics, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, 430022, PR China. Electronic address:

Background: Particulate matter≤ 2.5 μm (PM) is a type of environmental agent associated with air pollution, which induces hepatic fibrosis. However, the function and mechanism of PM on hepatic stellate cell (HSC) proliferation and fibrosis remain largely unknown.

Methods: Human HSC line (LX-2) and murine HSCs were exposed to various doses of PM. microRNA (miR)-411 expression was detected via quantitative reverse transcription polymerase chain reaction (qRT-PCR). Cell proliferation, fibrosis, mitochondrial dynamics dysfunction and mitophagy were determined via cell counting kit-8 (CCK-8), qRT-PCR, Western blotting and immunofluorescence.

Results: PM facilitated HSC proliferation and fibrosis via increasing the levels of ACTA2, Collagen 1, TIMP1 and TGF-β1. PM reduced miR-411 expression, and contributed to mitochondrial dynamics dysfunction via increasing Drp1 and decreasing OPA1, TOM20 and PGC-1α levels. PM promoted mitophagy by upregulating the levels of Beclin-1, LC3II/I, PINK1 and Parkin. miR-411 overexpression or autophagy blockage using 3-methyladenine (3-MA) relieved PM-mediated cell proliferation and fibrosis-associated factor expression in HSCs. Drp1 was targeted by miR-411. miR-411 mitigated PM-induced mitophagy via targeting Drp1. Drp1 overexpression abolished the inhibitory role of miR-411 in cell proliferation and fibrosis-associated factor levels in HSCs.

Conclusion: PM induced HSC activation and fibrosis via promoting Drp1-mediated mitophagy by decreasing miR-411, thereby causing liver fibrosis.
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http://dx.doi.org/10.1016/j.yexcr.2021.112828DOI Listing
September 2021

The Synergistic Anti-Tumor Activity of EZH2 Inhibitor SHR2554 and HDAC Inhibitor Chidamide through ORC1 Reduction of DNA Replication Process in Diffuse Large B Cell Lymphoma.

Cancers (Basel) 2021 Aug 24;13(17). Epub 2021 Aug 24.

Key Laboratory of Carcinogenesis and Translational Research (Ministry of Education), Department of Lymphoma, Peking University Cancer Hospital & Institute, No. 52 Fucheng Road, Haidian District, Beijing 100142, China.

Background: Upregulation of H3K27me3 induced by EZH2 overexpression or somatic heterozygous mutations were implicated in lymphomagenesis. It has been demonstrated that several EZH2-target agents have notable therapeutic effects in EZH2-mutant B-cell lymphoma patients. Here we present a novel highly selective EZH2 inhibitor SHR2554 and possible combination strategy in diffuse large B-cell lymphoma (DLBCL).

Methods: Cell proliferation, cell cycle and apoptosis were analyzed by CellTiter-Glo Luminescent Cell Viability Assay and flow cytometry. Western Blot was used to detect the expression of related proteins. The gene expression profiling post combination treatment was analyzed by RNA-Seq. Finally, CDX and PDX models were used to evaluate the synergistic anti-tumor effects of the combination treatment in vivo.

Results: The novel EZH2 inhibitor SHR2554 inhibited proliferation and induced G1 phase arrest in EZH2-mutant DLBCL cell lines. The combination of EZH2 inhibitor SHR2554 with histone deacetylase (HDAC) inhibitor chidamide (hereafter referred to as HBI8000) exerted synergistic anti-proliferative activity in vitro and in vivo. Gene expression profile analysis revealed dramatic inhibition of the DNA replication process in combined treatment.

Conclusions: SHR2554, a potent, highly selective small molecule inhibitor of EZH2, inhibited EZH2-mutant DLBCL more significantly in vitro and in vivo. The combination of HDAC inhibitor HBI8000 with EZH2 inhibitor SHR2554 exhibited dramatic anti-tumor activity in both mutant and wild-type DLBCL, which may become a potential therapeutic modality for the treatment of DLBCL patients.
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http://dx.doi.org/10.3390/cancers13174249DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8428225PMC
August 2021

Effect of river-lake connectivity on heavy metal diffusion and source identification of heavy metals in the middle and lower reaches of the Yangtze River.

J Hazard Mater 2021 08 20;416:125818. Epub 2021 Apr 20.

National Engineering Laboratory for Lake Pollution Control and Ecological Restoration, Institute of Lake Ecology and Environment, Chinese Research Academy of Environmental Sciences, Beijing 100012, China; State Environmental Protection Key Laboratory for Lake Pollution Control, Chinese Research Academy of Environmental Sciences, Beijing 100012, China. Electronic address:

Metal pollution poses a significant threat to ecological security and human health. Current research on the causes, sources and distribution of metal pollution in the Yangtze River plain is lacking. This study investigated the accumulation, risk, distribution, and sources of heavy metals in 62 lakes along the Yangtze River, and analyzed the relationship between river-lake connectivity, economic structure, population and metal diffusion. The mean concentrations of Cr, Cu, Hg, Zn, Cd, Pb and As in the surface sediments of these lakes were 90.8, 60.1, 0.06, 102, 0.89, 42.7, and 6.01 mg/kg, respectively. Most (99%) of the lake sediments were contaminated with Cd, and the lakes in the middle reach and southern bank of the Yangtze River had a higher ecological risk. Cr originated from the natural environment, whereas Zn, Cu, Pb, Cd and As were affected by human activities. The lakes disconnected from the Yangtze River had higher concentrations of Cu, Zn, Pb and As, while the lakes connected to the river had higher concentrations of Cd and Cr. This comprehensive analysis determined the pollution characteristics of heavy metals, illustrated the causes of non-point pollution in the Yangtze River plain, and showed that soil-water erosion is important in metal diffusion.
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http://dx.doi.org/10.1016/j.jhazmat.2021.125818DOI Listing
August 2021

Current Understanding of Osteoimmunology in Certain Osteoimmune Diseases.

Front Cell Dev Biol 2021 17;9:698068. Epub 2021 Aug 17.

State Key Laboratory of Oral Diseases and National Clinical Research Center for Oral Diseases, West China Hospital of Stomatology, Sichuan University, Chengdu, China.

The skeletal system and immune system seem to be two independent systems. However, there in fact are extensive and multiple crosstalk between them. The concept of osteoimmunology was created to describe those interdisciplinary events, but it has been constantly updated over time. In this review, we summarize the interactions between the skeletal and immune systems in the co-development of the two systems and the progress of certain typical bone abnormalities and bone regeneration on the cellular and molecular levels according to the mainstream novel study. At the end of the review, we also highlighted the possibility of extending the research scope of osteoimmunology to other systemic diseases. In conclusion, we propose that osteoimmunology is a promising perspective to uncover the mechanism of related diseases; meanwhile, a study from the point of view of osteoimmunology may also provide innovative ideas and resolutions to achieve the balance of internal homeostasis.
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http://dx.doi.org/10.3389/fcell.2021.698068DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8416088PMC
August 2021

Mitigation of urbanization effects on aquatic ecosystems by synchronous ecological restoration.

Water Res 2021 Aug 21;204:117587. Epub 2021 Aug 21.

Donghu Experimental Station of Lake Ecosystems, State Key Laboratory of Freshwater Ecology and Biotechnology of China, Institute of Hydrobiology, Chinese Academy of Sciences, No.7 Donghu South Road, Wuhan 430072, PR China; State Key laboratory of Marine Resource Utilization in South China Sea, Hainan University, Haikou, PR China. Electronic address:

Ecosystem degradation and biodiversity loss have been caused by economic booms in developing countries over recent decades. In response, ecosystem restoration projects have been advanced in some countries but the effectiveness of different approaches and indicators at large spatio-temporal scales (i.e., whole catchments) remains poorly understood. This study assessed the effectiveness of a diverse array of 440 aquatic restoration projects including wastewater treatment, constructed wetlands, plant/algae salvage and dredging of contaminated sediments implemented and maintained from 2007 to 2017 across more than 2000 km of the northwest Taihu basin (Yixing, China). Synchronized investigations of water quality and invertebrate communities were conducted before and after restoration. Our analysis showed that even though there was rapid urbanization at this time, nutrient concentrations (NH-N, TN, TP) and biological indices of benthic invertebrate (taxonomic richness, Shannon diversity, sensitive taxon density) improved significantly across most of the study area. Improvements were associated with the type of restoration project, with projects targeting pollution-sources leading to the clearest ecosystem responses compared with those remediating pollution sinks. However, in some locations, the recovery of biotic communities appears to lag behind nutrients (e.g., nitrogen and phosphorus), likely reflecting long-distance re-colonization routes for invertebrates given the level of pre-restoration degradation of the catchment. Overall, the study suggests that ecological damage caused by recent rapid economic development in China could potentially be mitigated by massive restoration investments synchronized across whole catchments, although these effects could be expected to be enhanced if urbanization rates were reduced at the same time.
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http://dx.doi.org/10.1016/j.watres.2021.117587DOI Listing
August 2021

Intelligent localization and quantitative evaluation of anterior talofibular ligament injury using magnetic resonance imaging of ankle.

BMC Med Imaging 2021 Aug 28;21(1):130. Epub 2021 Aug 28.

Radiology Department, Tianjin Hospital, 406 Jiefangnan Road, Hexi District, Tianjin, 300210, China.

Background: There is a high incidence of injury to the lateral ligament of the ankle in daily living and sports activities. The anterior talofibular ligament (ATFL) is the most frequent types of ankle injuries. It is of great clinical significance to achieve intelligent localization and injury evaluation of ATFL due to its vulnerability.

Methods: According to the specific characteristics of bones in different slices, the key slice was extracted by image segmentation and characteristic analysis. Then, the talus and fibula in the key slice were segmented by distance regularized level set evolution (DRLSE), and the curvature of their contour pixels was calculated to find useful feature points including the neck of talus, the inner edge of fibula, and the outer edge of fibula. ATFL area can be located using these feature points so as to quantify its first-order gray features and second-order texture features. Support vector machine (SVM) was performed for evaluation of ATFL injury.

Results: Data were collected retrospectively from 158 patients who underwent MRI, and were divided into normal (68) and tear (90) group. The positioning accuracy and Dice coefficient were used to measure the performance of ATFL localization, and the mean values are 87.7% and 77.1%, respectively, which is helpful for the following feature extraction. SVM gave a good prediction ability with accuracy of 93.8%, sensitivity of 88.9%, specificity of 100%, precision of 100%, and F1 score of 94.2% in the test set.

Conclusion: Experimental results indicate that the proposed method is reliable in diagnosing ATFL injury. This study may provide a potentially viable method for aided clinical diagnoses of some ligament injury.
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http://dx.doi.org/10.1186/s12880-021-00660-xDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8403355PMC
August 2021

Hemodynamic changes of anesthesia, pneumoperitoneum, and head-down tilt during laparoscopic surgery in elderly patients.

Ann Transl Med 2021 Jul;9(14):1177

The MOH Key Laboratory of Geriatrics, Beijing Hospital, National Center of Gerontology, Institute of Geriatric Medicine, Chinese Academy of Medical Sciences, Beijing, China.

Background: The hemodynamic variations of cardiac and cerebral blood monitoring during pneumoperitoneum and head-down tilt position in general anesthetized elderly patients remain unresolved. We evaluated the time course of cerebral tissue oxygen saturation (SctO) and cardiac output (CO) and investigated how the changes in hemodynamic values during the surgery would affect cerebral perfusion in elderly patients.

Methods: In this prospective observational study of 47 elderly patients (≥65 years old, American Society of Anesthesiologists Physical status I to III) undergoing laparoscopic colorectal radical resection with head-down position, SctO by near-infrared spectroscopy and arterial pressure-based cardiac output (APCO), Cardiac index (CI), stroke volume (SV), and SV index (SVI) according to FloTrac/Vigileo were measured at 9 time points. Heart rate (HR), mean arterial blood pressure (MAP), end-tidal carbon dioxide (ETCO), bispectral index (BIS), central venous pressure (CVP), and ventilator settings were recorded. Results are reported as medians [95% confidence interval (CI)].

Results: Heart Rate (HR), systolic blood pressure (SBP), diastolic blood pressure (DBP), MAP, CO, CI, SV, SVI, and SctO before incision decreased significantly compared with the waking state (P<0.05). SBP, CO, CI, SV, and SVI before incision decreased significantly compared with induction and intubation (P<0.05). SBP, DBP, MAP, and CVP increased significantly after pneumoperitoneum and head-down tilt, and then decreased during the following hour. CO and SVI decreased, while CI and SV increased after pneumoperitoneum and head-down tilt. CO, CI, SV and SVI decreased at the following 20, 40, and 60 minutes respectively. SctO increased after pneumoperitoneum and head-down tilt and remained stable during the following hour. CVP decreased while CO, CI, SV, and SVI increased significantly at the end of pneumoperitoneum and head-down tilt (P<0.05). HR and MAP increased significantly at the end of surgery compared to at the end of pneumoperitoneum and head-down tilt (P<0.05). CI was associated with SctO as indicated by a Pearson r of 0.035 (P<0.05).

Conclusions: Anesthesia, pneumoperitoneum, and head-down tilt affect cardiac function and cerebral perfusion in elderly patients. cardiac index independently affects elderly patients' cerebral blood flow.
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http://dx.doi.org/10.21037/atm-21-3407DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8350669PMC
July 2021

Associations of FSHR and LHCGR gene variants with ovarian reserve and clinical pregnancy rates.

Reprod Biomed Online 2021 Jun 29. Epub 2021 Jun 29.

Department of Laboratory Medicine, Sichuan Provincial People's Hospital, University of Electronic Science and Technology of China, Chengdu, China. Electronic address:

Research Question: Are there any associations between the variants of FSHR c.2039 G>A (p. Ser680Asn, rs6166) and LHCGR c.935A>G (p. Asn312Ser, rs2293275) and ovarian reserve, ovarian response, clinical pregnancy rate and POSEIDON group?

Design: A total of 210 infertile women were enrolled in this prospective study. The gene variants were analysed by the Sanger method. The clinical parameters were analysed based on genotypes.

Results: The frequency of heterozygous and homozygous G allele for FSHR c.2039 G>A in the low prognosis group was significantly higher than that in other response groups (P = 0.034); there was no significant association between LHCGR c.935 A>G and ovarian response. Moreover, the serum anti-Müllerian hormone (AMH) concentration, antral follicle count (AFC), oocytes retrieved, metaphase II (MII) oocytes and two-pronuclear (2PN) oocytes in patients with AG genotype for FSHR c.2039 G>A were significantly lower than those with AA genotype. The serum LH concentrations and clinical pregnancy rate of fresh embryo transfer in patients with GG genotype for LHCGR c.935 A>G were significantly higher than that of the AG genotype. In POSEIDON analysis, the low prognosis women with AA genotype for FSHR c.2039 G>A were more likely to appear in subgroup 1 (P = 0.038).

Conclusion: The FSHR c.2039 G>A variant has a significant beneficial influence on ovarian reserve and ovarian response. The LHCGR c.935 A>G variant is associated with increased clinical pregnancy rate of fresh embryo transfer in infertile women. In addition, the low prognosis women with AA genotype for FSHR c.2039 G>A tend to show better ovarian reserve and prognosis.
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http://dx.doi.org/10.1016/j.rbmo.2021.06.016DOI Listing
June 2021

Multi-omics network characterization reveals novel microRNA biomarkers and mechanisms for diagnosis and subtyping of kidney transplant rejection.

J Transl Med 2021 08 13;19(1):346. Epub 2021 Aug 13.

Institutes for Systems Genetics, Frontiers Science Center for Disease-Related Molecular Network, West China Hospital, Sichuan University, Chengdu, 610212, China.

Background: Kidney transplantation is an optimal method for treatment of end-stage kidney failure. However, kidney transplant rejection (KTR) is commonly observed to have negative effects on allograft function. MicroRNAs (miRNAs) are small non-coding RNAs with regulatory role in KTR genesis, the identification of miRNA biomarkers for accurate diagnosis and subtyping of KTR is therefore of clinical significance for active intervention and personalized therapy.

Methods: In this study, an integrative bioinformatics model was developed based on multi-omics network characterization for miRNA biomarker discovery in KTR. Compared with existed methods, the topological importance of miRNA targets was prioritized based on cross-level miRNA-mRNA and protein-protein interaction network analyses. The biomarker potential of identified miRNAs was computationally validated and explored by receiver-operating characteristic (ROC) evaluation and integrated "miRNA-gene-pathway" pathogenic survey.

Results: Three miRNAs, i.e., miR-145-5p, miR-155-5p, and miR-23b-3p, were screened as putative biomarkers for KTR monitoring. Among them, miR-155-5p was a previously reported signature in KTR, whereas the remaining two were novel candidates both for KTR diagnosis and subtyping. The ROC analysis convinced the power of identified miRNAs as single and combined biomarkers for KTR prediction in kidney tissue and blood samples. Functional analyses, including the latent crosstalk among HLA-related genes, immune signaling pathways and identified miRNAs, provided new insights of these miRNAs in KTR pathogenesis.

Conclusions: A network-based bioinformatics approach was proposed and applied to identify candidate miRNA biomarkers for KTR study. Biological and clinical validations are further needed for translational applications of the findings.
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http://dx.doi.org/10.1186/s12967-021-03025-8DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8361655PMC
August 2021

Relapsed/refractory classical Hodgkin lymphoma effectively treated with low-dose decitabine plus tislelizumab: A case report.

World J Clin Cases 2021 Jul;9(21):6041-6048

Department of Lymphoma, Key Laboratory of Carcinogenesis and Translational Research (Ministry of Education), Peking University Cancer Hospital & Institute, Beijing 100142, China.

Background: Academic studies have proved that anti-programmed death-1 (PD-1) monoclonal antibodies demonstrated remarkable activity in relapsed/refractory classical Hodgkin lymphoma (cHL). However, most patients ultimately experienced failure or resistance. It is urgent and necessary to develop a novel strategy for relapsed/refractory cHL. The aim of this case report is to evaluate the combination approach of low-dose decitabine plus a PD-1 inhibitor in relapsed/ refractory cHL patients with prior PD-1 inhibitor exposure.

Case Summary: The patient was a 27-year-old man who complained of enlarged right-sided cervical lymph nodes and progressive pain aggravation of the right shoulder over the past 3 mo before admission. Histological analysis of lymph node biopsy was suggestive of cHL. The patient experienced failure of eight lines of therapy, including multiple cycles of chemotherapy, PD-1 blockade, and anti-CD47 antibody therapy. Contrast-enhanced CT showed that the tumors of the chest and abdomen significantly shrunk or disappeared after three cycles of treatment with decitabine plus tislelizumab. The patient had been followed for 11.5 mo until March 2, 2021, and no progressive enlargement of the tumor was observed.

Conclusion: The strategy of combining low-dose decitabine with tislelizumab could reverse the resistance to PD-1 inhibitors in patients with heavily pretreated relapsed/ refractory cHL. The therapeutic effect of this strategy needs to be further assessed.
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http://dx.doi.org/10.12998/wjcc.v9.i21.6041DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8316945PMC
July 2021

RNA editing affects cis-regulatory elements and predicts adverse cancer survival.

Cancer Med 2021 Sep 28;10(17):6114-6127. Epub 2021 Jul 28.

Guizhou Provincial People's Hospital, Guiyang, China.

Background: RNA editing exerts critical impacts on numerous biological processes and thus are implicated in crucial human phenotypes, including tumorigenesis and prognosis. While previous studies have analyzed aggregate RNA editing activity at the sample level and associated it with overall cancer survival, there is not yet a large-scale disease-specific survival study to examine genome-wide RNA editing sites' prognostic value taking into account the host gene expression and clinical variables.

Methods: In this study, we solved comprehensive Cox proportional models of disease-specific survival on individual RNA-editing sites plus host gene expression and critical demographic covariates. This allowed us to interrogate the prognostic value of a large number of RNA-editing sites at single-nucleotide resolution.

Results: As a result, we identified 402 gene-proximal RNA-editing sites that generally predict adverse cancer survival. For example, an RNA-editing site residing in ZNF264 indicates poor survival of uterine corpus endometrial carcinoma, with a hazard ratio of 2.13 and an adjusted p-value of 4.07 × 10 . Some of these prognostic RNA-editing sites mediate the binding of RNA binding proteins and microRNAs, thus propagating their impacts to extensive regulatory targets.

Conclusions: In conclusion, RNA editing affects cis-regulatory elements and predicts adverse cancer survival.
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http://dx.doi.org/10.1002/cam4.4146DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8419749PMC
September 2021

Integration of Colloidal Quantum Dots with Photonic Structures for Optoelectronic and Optical Devices.

Adv Sci (Weinh) 2021 Sep 28;8(18):e2101560. Epub 2021 Jul 28.

Department of Electronic Engineering, The Chinese University of Hong Kong, Shatin, New Territories, Hong Kong SAR, China.

Colloidal quantum dot (QD), a solution-processable nanoscale optoelectronic building block with well-controlled light absorption and emission properties, has emerged as a promising material system capable of interacting with various photonic structures. Integrated QD/photonic structures have been successfully realized in many optical and optoelectronic devices, enabling enhanced performance and/or new functionalities. In this review, the recent advances in this research area are summarized. In particular, the use of four typical photonic structures, namely, diffraction gratings, resonance cavities, plasmonic structures, and photonic crystals, in modulating the light absorption (e.g., for solar cells and photodetectors) or light emission (e.g., for color converters, lasers, and light emitting diodes) properties of QD-based devices is discussed. A brief overview of QD-based passive devices for on-chip photonic circuit integration is also presented to provide a holistic view on future opportunities for QD/photonic structure-integrated optoelectronic systems.
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http://dx.doi.org/10.1002/advs.202101560DOI Listing
September 2021

Mussel-Inspired Design of a Self-Adhesive Agent for Durable Moisture Management and Bacterial Inhibition on PET Fabric.

Adv Mater 2021 Sep 23;33(35):e2100140. Epub 2021 Jul 23.

Research Centre of Smart Wearable Technology, Institute of Textiles and Clothing, The Hong Kong Polytechnic University, Hong Kong SAR, 999077, China.

Functional textiles with advanced moisture management can enhance human comfort and physiological health. However, conventional wet finishing processes used for textiles are usually highly polluting and exhibit poor fastness. Inspired by the strong underwater adhesion properties of mussels based on cation-π interaction, a novel superhydrophilic polymeric molecule with strong cohesion and adhesion property is designed on a poly(ethylene terephthalate) (PET) fabric. The cation-π hydrophilic agent (CPHA) can efficiently transform the hydrophobic PET fabric to a superhydrophilic one, and its superhydrophilicity can withstand 150 home laundry cycles. In addition, the cationic moieties in the CPHA self-adhere to the PET fabric without any finishing auxiliary that would cause pollution. Due to its strong adhesion, CPHA can be applied to one side of the PET fabric via spray coating and curing to form a Janus hydrophobic/superhydrophilic fabric capable of diode-like one-way sweat transportation (with forward transportation capability of 1115% and backward transportation capability of -1509%). Moreover, the Janus fabric inhibits bacterial growth and invasion, while simultaneously preserving the inner ecological healthy balance of the skin's microflora. This work opens up a pathway to develop adhesives in textile wet processing for more diverse, smarter applications, e.g., quick-dry sportswear, protective suits, or air-conditioning fabrics.
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http://dx.doi.org/10.1002/adma.202100140DOI Listing
September 2021

Efficacy and safety of PD-L1 inhibitors versus PD-1 inhibitors in first-line treatment with chemotherapy for extensive-stage small-cell lung cancer.

Cancer Immunol Immunother 2021 Jul 23. Epub 2021 Jul 23.

State Key Laboratory of Oncology in South China, Guangzhou, China.

Objectives: Programmed cell death-ligand 1 inhibitors plus chemotherapy (PD-L1 + Chemo) have achieved substantial progress in extensive-stage small-cell lung cancer (ES-SCLC). However, evidence about programmed cell death 1 inhibitors plus chemotherapy (PD-1 + Chemo) in SCLC is relatively lacking. Whether PD-1 inhibitors differ from PD-L1 inhibitors in their clinical outcomes remains controversial.

Materials And Methods: We performed a meta-analysis to compare efficacy and safety of PD-L1 + Chemo vs PD-1 + Chemo in ES-SCLC by searching PubMed, Embase, the Cochrane Library, and major oncology conferences. We examined overall survival (OS) as the primary outcome. Secondary outcomes included progression-free survival (PFS), objective response rate (ORR), and treatment-related adverse events (AEs).

Results: We included four randomized trials (IMpower133, CASPIAN, KEYNOTE-604, and EA5161) with a total of 1553 patients. Direct comparison showed that PD-L1 + Chemo (PFS: hazard ratio [HR] 0.79; OS: HR 0.75) and PD-1 + Chemo (PFS: HR 0.72; OS: HR 0.77) significantly prolonged survival time compared with chemotherapy alone. But PD-L1 + Chemo (relative risk [RR]: 1.07) and PD-1 + Chemo (RR: 1.13) were not superior to chemotherapy alone in terms of ORR. Indirect comparison showed no significant difference in clinical efficacy between PD-L1 + Chemo and PD-1 + Chemo (OS: HR 0.99; PFS: HR 1.10; ORR: RR 0.95). We further stratified patients according to subgroups in terms of OS. In the subgroup of patients with brain metastasis, PD-L1 + Chemo tended to prolong OS (HR: 0.61, 0.28 to 1.32). There were no significant differences between PD-L1 + Chemo and PD-1 + Chemo regarding safety analyses. However, PD-L1 + Chemo exhibited a better safety profile in reducing the risk of treatment discontinuation due to AEs (RR: 0.43, 0.19 to 0.95) and pneumonia (pneumonia of any grade, RR: 0.59, 0.24 to 1.42; pneumonia of grade ≥ 3, RR: 0.37, 0.10 to 1.39).

Conclusions: PD-L1 + Chemo and PD-1 + Chemo provided a significant survival benefit relative to chemotherapy alone for ES-SCLC. The efficacy and safety of PD-L1 + Chemo and PD-1 + Chemo were similar based on current evidence.
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http://dx.doi.org/10.1007/s00262-021-03017-zDOI Listing
July 2021

Nivolumab Plus Ipilimumab vs Nivolumab for Previously Treated Patients With Stage IV Squamous Cell Lung Cancer: The Lung-MAP S1400I Phase 3 Randomized Clinical Trial.

JAMA Oncol 2021 Sep;7(9):1368-1377

Yale Cancer Center, New Haven, Connecticut.

Importance: Nivolumab plus ipilimumab is superior to platinum-based chemotherapy in treatment-naive advanced non-small cell lung cancer (NSCLC). Nivolumab is superior to docetaxel in advanced pretreated NSCLC.

Objective: To determine whether the addition of ipilimumab to nivolumab improves survival in patients with advanced, pretreated, immunotherapy-naive squamous (Sq) NSCLC.

Design, Setting, And Participants: The Lung Cancer Master Protocol (Lung-MAP) S1400I phase 3, open-label randomized clinical trial was conducted from December 18, 2015, to April 23, 2018, randomizing patients in a 1:1 ratio to nivolumab alone or combined with ipilimumab. The median follow-up in surviving patients was 29.5 months. The trial was conducted through the National Clinical Trials Network and included patients with advanced immunotherapy-naive SqNSCLC and a Zubrod score of 0 (asymptomatic) to 1 (symptomatic but completely ambulatory) with disease progression after standard platinum-based chemotherapy. Randomization was stratified by sex and number of prior therapies (1 vs 2 or more). Data were analyzed from May 3, 2018, to February 1, 2021.

Interventions: Nivolumab, 3 mg/kg intravenously every 2 weeks, with or without ipilimumab, 1 mg/kg intravenously every 6 weeks, until disease progression or intolerable toxic effects.

Main Outcomes And Measures: The primary end point was overall survival (OS). Secondary end points included investigator-assessed progression-free survival (IA-PFS) and response per Response Evaluation Criteria in Solid Tumors (RECIST) guidelines, version 1.1.

Results: Of 275 enrolled patients, 252 (mean age, 67.5 years [range 41.8-90.3 years]; 169 men [67%]; 206 White patients [82%]) were deemed eligible (125 randomized to nivolumab/ipilimumab and 127 to nivolumab). The study was closed for futility at a planned interim analysis. Overall survival was not significantly different between the groups (hazard ratio [HR], 0.87; 95% CI, 0.66-1.16; P = .34). Median survival was 10 months (95% CI, 8.0-14.4 months) in the nivolumab/ipilimumab group and 11 months (95% CI, 8.6-13.7 months) in the nivolumab group. The IA-PFS HR was 0.80 (95% CI, 0.61-1.03; P = .09); median IA-PFS was 3.8 months (95% CI, 2.7-4.4 months) in the nivolumab/ipilimumab group and 2.9 months (95% CI, 1.8-4.0 months) in the nivolumab alone group. Response rates were 18% (95% CI, 12%-25%) with nivolumab/ipilimumab and 17% (95% CI, 10%-23%) with nivolumab. Median response duration was 28.4 months (95% CI, 4.9 months to not reached) with nivolumab/ipilimumab and 9.7 months with nivolumab (95% CI, 4.2-23.1 months). Grade 3 or higher treatment-related adverse events occurred in 49 of 124 patients (39.5%) who received nivolumab/ipilimumab and in 41 of 123 (33.3%) who received nivolumab alone. Toxic effects led to discontinuation in 31 of 124 patients (25%) on nivolumab/ipilimumab and in 19 of 123 (15%) on nivolumab.

Conclusions And Relevance: In this phase 3 randomized clinical trial, ipilimumab added to nivolumab did not improve outcomes in patients with advanced, pretreated, immune checkpoint inhibitor-naive SqNSCLC.

Trial Registration: ClinicalTrials.gov Identifier: NCT02785952.
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http://dx.doi.org/10.1001/jamaoncol.2021.2209DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8283667PMC
September 2021

Management of systemic risk factors ahead of dental implant therapy: A beard well lathered is half shaved.

J Leukoc Biol 2021 09 7;110(3):591-604. Epub 2021 Jul 7.

State Key Laboratory of Oral Diseases and National Clinical Research Center for Oral Diseases, West China Hospital of Stomatology, Sichuan University, Chengdu, China.

As the most successful therapy for missing teeth, dental implant has become increasingly prevalent around the world. A lot of papers have reported diverse local risk factors affecting the success and survival rate of dental implants, either for a short or a long period. However, there are also many types of systemic disorders or relatively administrated medicine that may jeopardize the security and success of dental implant treatment. Additionally, the coronavirus disease 2019 pandemic also poses a challenge to dental implant clinicians. Some of these risk factors are clinically common but to some extent unfamiliar to dentists, thus optimal measurements are often lacking when they occur in dental clinics. In this review, we analyze potential systemic risk factors that may affect the success rate of dental implants. Some of them may affect bone mineral density or enhance the likelihood of local infection, thus impeding osseointegration. Others may even systemically increase the risk of the surgery and threaten patients' life. In order to help novices receive high-risk patients who need to get dental implant treatment in a more reasonable way, we accordingly review recent research results and clinical experiments to discuss promising precautions, such as stopping drugs that impact bone mineral density or the operation, and addressing any perturbations on vital signs.
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http://dx.doi.org/10.1002/JLB.6MR0621-760RRDOI Listing
September 2021

Characterization, Sources and Excessive Cancer Risk of PM-Bound Polycyclic Aromatic Hydrocarbons in Different Green Spaces in Lin'an, Hangzhou, China.

Bull Environ Contam Toxicol 2021 Sep 6;107(3):519-529. Epub 2021 Jul 6.

College of Landscape Architecture, Zhejiang Agriculture and Forestry University, Hangzhou, 311300, Zhejiang, China.

PM samples were collected from residential, commercial, plaza and public green spaces in Lin'an, Hangzhou, in spring (March and April) and winter (February and December) in 2017. PAHs were detected by gas chromatography-mass spectrometry (GC-MS), and their sources were identified using the diagnostic ratio (DR) and principal component analysis-multiple linear regression (PCA-MLR). The average PAH concentration in winter was 1.3 times that in spring (p < 0.01). The PAH concentrations in the green spaces decreased as commercial > residential > plaza > public green space (p < 0.05). The sources of PAHs were vehicle emissions and coal combustion pollution transported by northern Chinese air masses. Slightly higher excessive cancer risks were determined in the commercial and residential green spaces than in the plaza and public green spaces. Green coverage, pedestrian volume, traffic flow and building density greatly influenced the decrease in the PAH concentration in the green spaces. Among the 4 types of green spaces, public green space had the most ecological benefits and should be fully utilized in urban green space planning to improve public health in urban spaces.
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http://dx.doi.org/10.1007/s00128-021-03304-6DOI Listing
September 2021

Low miR-1231 expression predicts poor prognosis in non-small-cell lung cancer and accelerates cell proliferation, migration and invasion.

Biomark Med 2021 Aug 5;15(11):831-840. Epub 2021 Jul 5.

Department of Pulmonary Medicine, Affiliated Hospital of Jiangsu University, Zhenjiang, 212001, China.

miRNAs have been found to be involved in the tumor progression. This study aimed to assess the prognostic significance and biological function of miRNA-1231 (miR-1231) in non-small-cell lung cancer (NSCLC). Expression of miR-1231 was measured by using quantitative real-time PCR. The prognosis value of miR-1231 was evaluated by Kaplan-Meier survival curves and Cox regression analysis. The biological function of miR-1231 was further studied. Expression of miR-1231 in NSCLC patients and NSCLC cell lines were decreased. MiR-1231 was an independent prognostic biomarker. Overexpression of miR-1231 inhibited NSCLC cell proliferation, migration and invasion. Downregulated expression of miR-1231 serves as a prognostic biomarker of NSCLC and may be a potential therapeutic target.
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http://dx.doi.org/10.2217/bmm-2020-0313DOI Listing
August 2021

Geometric Modulation of Local CO Flux in [email protected] O Nanoreactors for Steering the CO RR Pathway toward High-Efficacy Methane Production.

Adv Mater 2021 Aug 5;33(32):e2101741. Epub 2021 Jul 5.

Soochow Institute of Energy and Material Innovations, College of Energy, Jiangsu Key Laboratory for Advanced Carbon Materials and Wearable Energy Technologies, Soochow University, Suzhou, 215006, China.

The electroreduction of carbon dioxide (CO RR) to CH stands as one of the promising paths for resourceful CO utilization in meeting the imminent "carbon-neutral" goal of the near future. Yet, limited success has been witnessed in the development of high-efficiency catalysts imparting satisfactory methane selectivity at a commercially viable current density. Herein, a unique category of CO RR catalysts is fabricated with the yolk-shell nanocell structure, comprising an Ag core and a Cu O shell that resembles the tandem nanoreactor. By fixing the Ag core and tuning the Cu O envelope size, the CO flux arriving at the oxide-derived Cu shell can be regulated, which further modulates the *CO coverage and *H adsorption at the Cu surface, consequently steering the CO RR pathway. Density functional theory simulations show that lower CO coverage favors methane formation via stabilizing the intermediate *CHO. As a result, the best catalyst in the flow cell shows a high CH Faraday efficiency of 74 ± 2% and partial current density of 178 ± 5 mA cm at -1.2 V , ranking above the state-of-the-art catalysts reported today for methane production. These findings mark the significance of precision synthesis in tailoring the catalyst geometry for achieving desired CO RR performance.
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http://dx.doi.org/10.1002/adma.202101741DOI Listing
August 2021

Identification of novel genomic regions associated with nine mineral elements in Chinese winter wheat grain.

BMC Plant Biol 2021 Jul 1;21(1):311. Epub 2021 Jul 1.

State Key Laboratory of Crop Biology, Key Laboratory of Crop Biology of Shandong Province, Group of Wheat Quality and Molecular Breeding, College of Agronomy, Shandong Agricultural University, Tai'an, Shandong, 271000, P.R. China.

Background: Mineral elements are important for maintaining good human health besides heavy metals. Mining genes that control mineral elements are paramount for improving their accumulation in the wheat grain. Although previous studies have reported some loci for beneficial trace elements, they have mainly focused on Zn and Fe content. However, little information is available regarding the genetic loci differences in dissecting synchronous accumulation of multiple mineral elements in wheat grains, including beneficial and heavy elements. Therefore, a genome-wide association study (GWAS) was conducted on 205 wheat accessions with 24,355 single nucleotide polymorphisms (SNPs) to identify important loci and candidate genes for controlling Ca, Fe, Zn, Se, Cu, Mn, Cd, As, and Pb accumulation in wheat grains.

Results: A total of 101 marker-trait associations (MTAs) (P < 10) loci affecting the content of nine mineral elements was identified on chromosomes 1B, 1D, 2A, 2B, 3A, 3B, 3D, 4A, 4B, 5A, 5B, 5D, 6B, 7A, 7B, and 7D. Among these, 17 major MTAs loci for the nine mineral elements were located, and four MTAs loci (P < 10) were found on chromosomes 1B, 6B, 7B, and 7D. Eight multi-effect MTAs loci were detected that are responsible for the control of more than one trait, mainly distributed on chromosomes 3B, 7B, and 5A. Furthermore, sixteen candidate genes controlling Ca, Fe, Zn, Se, Cd, and Pb were predicted, whose functions were primarily related to ion binding, including metals, Fe, Ca, Cu, Mg, and Zn, ATP binding, ATPase activity, DNA binding, RNA binding, and protein kinase activity.

Conclusions: Our study indicated the existence of gene interactions among mineral elements based on multi-effect MTAs loci and candidate genes. Meanwhile this study provided new insights into the genetic control of mineral element concentrations, and the important loci and genes identified may contribute to the rapid development of beneficial mineral elements and a reduced content of harmful heavy metals in wheat grain.
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http://dx.doi.org/10.1186/s12870-021-03105-3DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8252321PMC
July 2021

Characterization of Tumor-Associated Macrophages and the Immune Microenvironment in Limited-Stage Neuroendocrine-High and -Low Small Cell Lung Cancer.

Biology (Basel) 2021 Jun 4;10(6). Epub 2021 Jun 4.

Department of Tumor Biology, National Korányi Institute of Pulmonology, Piheno ut 1, 1121 Budapest, Hungary.

This study aims to characterize tumor-infiltrating macrophages (TAMs), myeloid-derived suppressor cells (MDSC), and the related molecular milieu regulating anti-tumor immunity in limited-stage neuroendocrine (NE)-high and NE-low small cell lung cancer. Primary tumors and matched lymph node (LN) metastases of 32 resected, early-stage SCLC patients were analyzed by immunohistochemistry (IHC) with antibodies against pan-macrophage marker CD68, M2-macrophage marker CD163, and MDSC marker CD33. Area-adjusted cell counting on TMAs showed that TAMs are the most abundant cell type in the TME, and their number in tumor nests exceeds the number of CD3 + T-cells (64% vs. 38% in NE-low and 71% vs. 18% in NE-high). Furthermore, the ratio of CD163-expressing M2-polarized TAMs in tumor nests was significantly higher in NE-low vs. NE-high tumors (70% vs. 31%). TAM density shows a strong positive correlation with CD45 and CD3 in tumor nests, but not in the stroma. fGSEA analysis on a targeted RNAseq oncological panel of 2560 genes showed that NE-high tumors exhibited increased enrichment in pathways related to cell proliferation, whereas in NE-low tumors, immune response pathways were significantly upregulated. Interestingly, we identified a subset of NE-high tumors representing an immune-oasis phenotype, but with a different gene expression profile compared to NE-low tumors. In contrast, we found that a limited subgroup of NE-low tumors is immune-deserted and express distinct cellular pathways from NE-high tumors. Furthermore, we identified potential molecular targets based on our expression data in NE-low and immune-oasis tumor subsets, including CD70, ANXA1, ITGB6, TP63, IFI27, YBX3 and CXCR2.
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http://dx.doi.org/10.3390/biology10060502DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8228874PMC
June 2021

Temporal dynamics of teleost populations during the Pleistocene: a report from publicly available genome data.

BMC Genomics 2021 Jun 30;22(1):490. Epub 2021 Jun 30.

Shenzhen Key Lab of Marine Genomics, Guangdong Provincial Key Lab of Molecular Breeding in Marine Economic Animals, BGI Academy of Marine Sciences, BGI Marine, BGI, Shenzhen, Guangdong, China.

Background: Global climate oscillation, as a selection dynamic, is an ecologically important element resulting in global biodiversity. During the glacial geological periods, most organisms suffered detrimental selection pressures (such as food shortage and habitat loss) and went through population declines. However, during the mild interglacial periods, many species re-flourished. These temporal dynamics of effective population sizes (N) provide essential information for understanding and predicting evolutionary outcomes during historical and ongoing global climate changes.

Results: Using high-quality genome assemblies and corresponding sequencing data, we applied the Pairwise Sequentially Markovian Coalescent (PSMC) method to quantify N changes of twelve representative teleost species from approximately 10 million years ago (mya) to 10 thousand years ago (kya). These results revealed multiple rounds of population contraction and expansion in most of the examined teleost species during the Neogene and the Quaternary periods. We observed that 83% (10/12) of the examined teleosts had experienced a drastic decline in N before the last glacial period (LGP, 110-12 kya), slightly earlier than the reported pattern of N changes in 38 avian species. In comparison with the peaks, almost all of the examined teleosts maintained long-term lower N values during the last few million years. This is consistent with increasingly dramatic glaciation during this period.

Conclusion: In summary, these findings provide a more comprehensive understanding of the historical N changes in teleosts. Results presented here could lead to the development of appropriate strategies to protect species in light of ongoing global climate changes.
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http://dx.doi.org/10.1186/s12864-021-07816-7DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8247217PMC
June 2021

Gastric emptying of preoperative drinks is slower in adults with chronic energy deficiency: A 2 hour cross-over study among Chinese.

Asia Pac J Clin Nutr 2021 Jun;30(2):206-212

Department of Nutrition, General Hospital of Southern Theater Command, PLA, Guangzhou, China. Email:

Background And Objectives: To investigate the effects of oral preoperative regimens on gastric emptying time in relation to BMI in Chinese adults.

Methods And Study Design: The enrolled 56 adults were divided into three groups (normal-weight, underweight, and overweight) and completed a regimen of two drinks after a 2-week interval. After drinking a carbohydrate regimen (CD, 50 g carbohydrates) or a carbohydrate glutamine regimen (CGD, 44 g carbohydrates and 6 g glutamine) labelled with 99mTc-DTPA (99mTc-diethylenetriaminepentaacetic acid), gastric emptying times T50 and T90 were measured using a curve derived from scintigraphic images.

Results: T50 and T90 had no significant difference between the CD and CGD regimens. T50 was significantly delayed in the underweight participants (BMI <18.5 kg/m2, as Chronic Energy Deficiency, CED) compared with the normal-weight participants after drinking CD (p=0.003) or CGD (p=0.002), as well as T90 after CD (p=0.019). There was no difference in glucose concentrations between the three groups. There are negative correlations between body weight and gastric emptying time T50 (r=-0.461, p=0.016) or T90 (r=-0.553, p=0. 003) after drinking CD, as well as T50 (r=-0.553, p=0.003) after drinking CGD.

Conclusions: Underweight adults should be careful to take oral preoperative regimens 2 hours before surgery and consider reducing the volume because of a slower gastric emptying rate.
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http://dx.doi.org/10.6133/apjcn.202106_30(2).0004DOI Listing
June 2021

Predictive value of computed tomography with coronal reconstruction in right hemicolectomy with complete mesocolic excision for right colon cancers: a retrospective study.

World J Surg Oncol 2021 Jun 28;19(1):189. Epub 2021 Jun 28.

Department of Gastrointestinal Surgical Oncology, Fujian Cancer Hospital & Fujian Medical University Cancer Hospital, 420 Fuma Street, Jinan, Fuzhou, Fujian, 350014, P.R. China.

Background: Understanding the vascular anatomy is critical for performing central vascular ligation (CVL) in right hemicolectomy with complete mesocolic excision (CME). This study aimed to investigate the predictive value of multi-slice spiral computed tomography (MSCT) with coronal reconstruction in right hemicolectomy with CME.

Methods: This is a retrospective descriptive study. Eighty patients with right colon cancer who underwent right hemicolectomy from December 2015 to January 2020 were included. The intraoperative reports (including imaging data) and MSCT images with coronal reconstruction were analysed and compared. The detection rates of the ileocolic vein (ICV) and ileocolic artery (ICA) roots and the accuracy in predicting their anatomical relationship were analysed. The detection rate and accuracy in predicting the location of the gastrocolic trunk of Henle (GTH), middle colic artery (MCA) and middle colic vein (MCV) were analysed. The distance from the ICV root to the GTH root (ICV-GTH distance) was measured and analysed. The maximum distance from the left side of the superior mesenteric artery (SMA) to the right side of the superior mesenteric vein (SMV), named the 'lsSMA-rsSMV distance', was also measured and analysed.

Results: In seventy-four (92.5%) patients, both the ICV and ICA roots were located; their anatomical relationship was determined by MSCT, and the accuracy of the prediction was 97.2% (72/74). The GTH was located by MSCT in 75 (93.7%) patients, and the accuracy of the prediction was 97.33% (73/75). The MCA was located by MSCT in 47 (58.75%) patients, and the accuracy was 78.72% (37/47). The MCV was located by MSCT in 51 (63.75%) patients, and the accuracy of the prediction was 84.31% (43/51). The ICV-GTH distance was measured in 73 (91.2%) patients, and the mean distance was 4.28 ± 2.5 cm. The lsSMA-rsSMV distance was measured in 76 (95%) patients, and the mean distance was 2.21 ± 0.6 cm.

Conclusions: With its satisfactory accuracy in predicting and visualising the information of key anatomical sites, MSCT with coronary reconstruction has some predictive value in CME with CVL in right hemicolectomy.
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http://dx.doi.org/10.1186/s12957-021-02307-1DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8240319PMC
June 2021

Single-Ion Conducting Double-Network Hydrogel Electrolytes for Long Cycling Zinc-Ion Batteries.

ACS Appl Mater Interfaces 2021 Jul 24;13(26):30594-30602. Epub 2021 Jun 24.

Nano Center, Institute of Textiles & Clothing, Hong Kong Polytechnic University, Hong Kong, China.

As one of the promising alternatives of lithium-ion batteries, zinc-ion batteries (ZIBs) have received growing interest from researchers due to their good safety, eco-friendliness, and low cost. Nevertheless, aqueous ZIBs are still a step away from practical applications due to the nonuniform deposition of Zn and parasitic side reactions, which cause capacity fading and even short circuit. To tackle these problems, here we introduce a single-Zn-ion conducting hydrogel electrolyte (SIHE), P(ICZn-AAm), synthesized with iota carrageenan (IC) and acrylamide (AAm). The SIHE manifests single Zn conductivity via the abundant sulfates fixed on the IC polymer backbone, delivering a high Zn transference number of 0.93. It also exhibits outstanding ionic conductivity of 2.15 × 10 S cm at room temperature. The enhanced compatibility at the electrode-electrolyte interface was verified by the stable Zn striping/plating performance along with a homogenous and smooth Zn deposition layer. It is also found that the passivation of the Zn anode can be effectively prohibited due to the lack of free anions in the electrolyte. The practical performance of the SIHE is further investigated with Zn-VO batteries, which showed a stable capacity of 271.6 mA h g over 150 cycles at 2 C and 127.5 mA h g over 500 cycles at 5 C.
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http://dx.doi.org/10.1021/acsami.1c05941DOI Listing
July 2021

A Robust and Rapid Candidate Gene Mapping Pipeline Based on M2 Populations.

Front Plant Sci 2021 2;12:681816. Epub 2021 Jun 2.

Key Laboratory of Soybean Molecular Design Breeding, Northeast Institute of Geography and Agroecology, The Innovative Academy of Seed Design, Chinese Academy of Sciences, Changchun, China.

The whole-genome sequencing-based bulked segregant analysis (WGS-BSA) has facilitated the mapping candidate causal variations for cloning target plant genes. Here, we report an improved WGS-BSA method termed as M2-seq to expedite the mapping candidate mutant loci by studying just M generation. It is an efficient mutant gene mapping tool, rapid, and comparable to the previously reported approaches, such as Mutmap and Mutmap+ that require studying M or advanced selfed generations. In M2-seq, background variations among the M populations can be removed efficiently without knowledge of the variations of the wild-type progenitor plant. Furthermore, the use of absolute delta single-nucleotide polymorphism (SNP) index values can effectively remove the background variation caused by repulsion phase linkages of adjacent mutant alleles; and thereby facilitating the identification of the causal mutation in target genes. Here, we demonstrated the application of M2-seq in successfully mapping the genomic regions harboring causal mutations for mutant phenotypes among 10 independent M populations of soybean. The mapping candidate mutant genes just in M generation with the aid of the M2-seq method should be particularly useful in expediting gene cloning especially among the plant species with long generation time.
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http://dx.doi.org/10.3389/fpls.2021.681816DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8207192PMC
June 2021

Sn modified nanoporous Ge for improved lithium storage performance.

J Colloid Interface Sci 2021 Nov 9;602:563-572. Epub 2021 Jun 9.

School of Materials Science and Engineering, Hebei University of Technology, Tianjin 300401, China; Key Laboratory for New Type of Functional Materials in Hebei Province, Hebei University of Technology, Tianjin 300401, China; Research Institute of Foundry, Hebei University of Technology, Tianjin 300401, China. Electronic address:

Although high-capacity germanium (Ge) has been regarded as the promising anode material for lithium ion batteries (LIBs), its actual performance is far from expectation because of low electrical conductivity and rapid capacity decay during cycling. In this work, Sn modified nanoporous Ge materials with different Ge/Sn atomic ratios in precursors were synthesized by a simple melt-spinning and dealloying strategy. As the anodes of LIBs, Sn modified nanoporous Ge materials display improved cycling stability compared with Sn-free nanoporous Ge, revealing a potential role of Sn in improving electrochemical properties of Ge-based anodes. In particular, Sn modified nanoporous Ge with Ge/Sn atomic ratio of 3:1 presents the best Li storage performance among measured electrodes, delivering a reversible capacity of 974 mA h g after 500 cycles at 200 mA g. It is found that the introduction of appropriate amount of Sn can not only regulate the nanoporous structure of Ge to better alleviate volume expansion, but also improves the conductivity and activity of the electrode material. This improvement is demonstrated by density functional theory calculations. The study uncovers a route to improve Li storage properties by rationally modify Ge-based anodes with Sn, which may facilitate the development of high-performance LIBs.
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http://dx.doi.org/10.1016/j.jcis.2021.06.046DOI Listing
November 2021

Dual-step irradiation strategy to sequentially destroy singlet oxygen-responsive polymeric micelles and boost photodynamic cancer therapy.

Biomaterials 2021 08 11;275:120959. Epub 2021 Jun 11.

Key Laboratory of Biomedical Polymers of Ministry of Education, Department of Chemistry, Wuhan University, Wuhan, 430072, China. Electronic address:

Nanotechnology provides a powerful tool to overcome many disadvantages of small-molecule photosensitizers for photodynamic cancer therapy, such as hydrophobicity, rapid blood clearance, low accumulation in tumor tissue and low cell penetration, etc. The occurrence of quench in photosensitizer-loaded nanoparticle greatly downregulates the ability to generate singlet oxygen with light irradiation. Stimuli-responsive nanocarriers can improve the efficacy of PDT to a certain extent. However, insufficient release of photosensitizer from either endogenous or exogenous stimuli responsive nanocarriers in the short period of light irradiation restricts full usage of the photosensitizer delivered into cancer cells. We here report a dual-step light irradiation strategy to enhance the efficacy of cancer PDT. Ce6 as a photosensitizer is loaded in singlet oxygen-sensitive micelles (Ce6-M) via self-assembly of amphiphilic polymer mPEG-TK-C. After co-incubation of Ce6-M with cancer cells or i.v. injection of Ce6-M, cancer cells or tumor tissues are irradiated with light for a short time to trigger Ce6 release, and 2 h later, re-irradiated for relatively long time. The sufficient release of Ce6 in the period between twice light irradiation significantly improves the generation of singlet oxygen, leading to more efficient cancer therapeutic effects of dual-step irradiation than that of single-step irradiation for the same total irradiation time.
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http://dx.doi.org/10.1016/j.biomaterials.2021.120959DOI Listing
August 2021
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