Publications by authors named "Hui Xu"

2,835 Publications

  • Page 1 of 1

miR-548 predicts clinical prognosis and functions as a tumor promoter in gastric cancer.

Clin Exp Med 2022 Aug 17. Epub 2022 Aug 17.

Department of Digestive Internal Medicine, Seventh People's Hospital of Shanghai University of TCM, 358 Gaoqiao Datong Road, Shanghai, 200137, China.

Numerous pieces of evidence have demonstrated the functional role of miR-548 in various cancers. The expression and function of miR-548 in gastric cancer were investigated in the present study. A total of 123 gastric cancer patients were included and provided paired gastric cancer tissues and matched normal tissues. RT-qPCR was used to detect the expression of miR-548. CCK8 assay was used to evaluate cell proliferation, and Transwell assay was applied to assess cell migration and invasion. The clinical significance of miR-548 was estimated by a series of statistical analyses. miR-548 was found to be upregulated in gastric cancer, which was associated with the lymph node metastasis and TNM stage of patients. Patients with relatively high miR-548 expression possessed bad survival. miR-548 was identified as a prognostic indicator of gastric cancer. miR-548 was also found to promote the proliferation, migration, and invasion of gastric cancer. Upregulated miR-548 was involved in the progression of gastric cancer and predicted the prognosis of patients. Inhibition of miR-548 might be a novel therapeutic strategy for gastric cancer.
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http://dx.doi.org/10.1007/s10238-022-00870-9DOI Listing
August 2022

FOXN3 expression regulated by miR-299-5p inhibiting the proliferation, migration and invasion of oral squamous cell carcinoma cells.

Protein Pept Lett 2022 Aug 16. Epub 2022 Aug 16.

Department of Maxillofacial Surgery, People\'s Hospital of Xinjiang Uygur Autonomous Region, Urumqi 830001, Xinjiang, China.

Background: Oral squamous cell carcinoma (OSCC) is one of the commonest malignancies of the oral cavity. FOXN3 is a tumor suppressor that represses the progression of many tumors. Nonetheless, its role in OSCC has not been elucidated. This work is performed to probe the role and dysregulation mechanism of FOXN3 in OSCC.

Method: FOXN3 mRNA and miR-299-5p expressions were quantified by quantitative real-time polymerase chain reaction (qRT-PCR); 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay was employed to detect OSCC cell growth; Transwell experiment was conducted to detect cell migration and invasion; dual-luciferase reporter experiment and bioinformatics were adopted to analyze the relationship between miR-299-5p and FOXN3; Western blot was implemented to detect FOXN3 protein expression.

Result: FOXN3 expression was remarkably down-modulated, and miR-299-5p expression was markedly up-modulated in OSCC tissues and cell lines compared with paracancerous tissues and normal oral epithelial cell line. FOXN3 overexpression impeded OSCC cell growth, migration and invasion. FOXN3 was proven to be a downstream target of miR-299-5p, and miR-299-5p mimics enhanced OSCC cell growth, migration and invasion. Moreover, FOXN3 overexpression partially reversed the promoting effects of miR-299-5p mimics on OSCC cell growth, migration and invasion.

Conclusion: FOXN3 expression is remarkably down-modulated in OSCC tissues and cell lines, and miR-299-5p targets FOXN3 to facilitate OSCC cell growth, migration and invasion. These results imply that miR-299-5p/FOXN3 axis may be a potential target for OSCC treatment.
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http://dx.doi.org/10.2174/0929866529666220816143538DOI Listing
August 2022

The value of interleukin-27 for differentiating tuberculous pleural effusion from pneumonic effusion in children.

Front Pediatr 2022 28;10:948862. Epub 2022 Jul 28.

Department of Respiratory Medicine, Beijing Children's Hospital, Capital Medical University, National Center for Children's Health, Beijing, China.

Objectives: The early diagnosis of tuberculous pleural effusion (TPE) is challenging due to the difficulty of isolating , and pleural biomarkers are an optional choice. Recent studies showed that interleukin-27 (IL-27) appears to be a new accurate biomarker for TPE in adults and no related studies were reported in children. In this study, we aimed to evaluate the potential value of IL-27 in pediatric tuberculous pleurisy by detecting its levels in pleural fluid and serum.

Methods: A total of 48 children with TPE and 64 children with severe (MP) pneumonic effusion (SMPPE) were enrolled in this study. IL-27 concentrations were measured in serum and pleural fluid. The diagnostic yield of IL-27 was evaluated using receiver operating characteristic (ROC) curves.

Results: The level of p-IL-27 in TPE showed statistically no significant difference when compared with SMPPE ( > 0.05). However, pleural fluid IL-27 (p-IL-27) / serum IL-27 (s-IL-27) ratio in TPE were significantly much higher than those in SMPPE ( < 0.05). By the analysis of the ROC curves, the diagnostic sensitivity and specificity of the p-IL-27/s-IL-27 ratio were 100% and 48.44%, respectively (cutoff value of 1.0280). The area under the ROC curve for p-IL-27/s-IL-27 was 0.7295.

Conclusion: Pleural fluid IL-27 alone was not accurate in distinguishing pediatric TPE from SMPPE, which was different from the diagnostic value of IL-27 in adult studies due to the different disease spectra between children and adults. Our results implied that the p-IL-27/s-IL-27 ratio had a potential value in distinguishing TPE from SMPPE. However, the specificity of IL-27 was relatively lower and it is necessary to find a more specific marker in tuberculous pleurisy of children.
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http://dx.doi.org/10.3389/fped.2022.948862DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9367962PMC
July 2022

Expanded CD1cCD163 DC3 Population in Synovial Tissues Is Associated with Disease Progression of Osteoarthritis.

J Immunol Res 2022 1;2022:9634073. Epub 2022 Aug 1.

Shanghai University of Traditional Chinese Medicine, Shanghai, China.

The mechanisms underlying osteoarthritis (OA) have recently been hypothesized to involve a dysfunctional immune system. In this study, we collected synovium, synovial fluid (SF), and peripheral blood from 21 patients. Mononuclear cells were characterized using FCM. H&E staining and mIHC histological assessment of synovium were performed. Cytokine levels in the SF were measured using ELISA. We observed similar frequencies of immune cells in the synovium and SF, which were enriched in DCs. Notably, CD1cCD163 DC3s were expanded in the synovium and SF. Furthermore, we found that DC3s were primarily located within the ectopic lymphoid-like structure (ELLS) in close proximity to CD8 T cells. Finally, the level of TNF- and IL12p70 in the SF correlated with the severity of OA. These data suggest that OA is an immune system-related disease and that DC3s may play an active role in OA progression by promoting ELLS formation and inflammatory responses.
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http://dx.doi.org/10.1155/2022/9634073DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9359855PMC
August 2022

Excessive Oxalic Acid Secreted by Inhibits the Growth of Mycelia during Its Saprophytic Process.

Cells 2022 Aug 5;11(15). Epub 2022 Aug 5.

College of Horticulture, Shenyang Agricultural University, Shenyang 110866, China.

is an edible and medicinal mushroom in Asia commercially cultivated on substrates containing pine sawdust. Its slow mycelial growth rate greatly increases the cultivation cycle. In this study, we mainly studied the role of oxalic acid (OA) secreted by in its saprophytic process. Our results show that crystals observed on the mycelial surface contained calcium oxalate monohydrate (COM) and calcium oxalate dihydrate (COD) according to X-ray diffraction (XRD). Vegetative mycelia secreted large amounts of OA during extended culture periods. However, high concentrations of OA decreased the mycelial growth rate significantly. Moreover, the degradation of lignocellulose was significantly inhibited under high concentrations of OA. These changes could be attributed to the significantly decreased activities of lignocellulose-degrading enzymes. In conclusion, by establishing a link between OA secretion by the mycelium and the slow growth rate of its saprophytic process, this work provides fundamental information for shortening the cultivation cycle of .
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http://dx.doi.org/10.3390/cells11152423DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9368360PMC
August 2022

Effects of soil moisture, net radiation, and atmospheric vapor pressure deficit on surface evaporation fraction at a semi-arid grass site.

Sci Total Environ 2022 Aug 6;849:157890. Epub 2022 Aug 6.

Key Laboratory of Geospatial Technology for the Middle and Lower Yellow River Regions (Henan University), Ministry of Education, Kaifeng, China.

Surface energy partitioning is one of the most important aspects of the land-atmosphere coupling. The objective of this study is to examine how soil moisture (SM) and atmospheric conditions (net radiation, Rn and vapor pressure deficit, VPD) affect surface evaporation fraction (EF, determined by LE/(LE + H), where LE and H are latent and sensible heat flux, respectively) with measurements at a semi-arid grass site in China during the mid-growing season, 2020. The three factors (SM, Rn, and VPD) were divided into different levels, and then their effects on EF were investigated qualitatively using a combinatorial stratification method and quantificationally using a path analysis. Generally, the results indicated that the effect of one factor of SM, Rn and VPD on EF was influenced by the other two factors. EF tended to increase with increasing SM. Increased VPD (Rn) enhanced (weakened) the SM-EF relationship. When soil was dry, EF tended to decrease with increasing VPD; when soil was wet, EF initially levelled off and then decreased with increasing VPD. Increased Rn enhanced (weakened) the positive (negative) effect of VPD on EF when soil was wet (dry). In terms of Rn effect, EF tended to decrease as Rn increases. Further, path analysis suggested that SM, Rn, and VPD not only directly affected EF, but also indirectly affected EF, mainly through canopy conductance (Gs) and temperature difference between land surface and air (∆T). The direct effect of SM accounted for >50 % of its total effect on EF, while the total effects of Rn and VPD on EF were dominated by their indirect effects. These observational evidences may have implications for improving representation of land-atmosphere coupling in atmospheric general circulation models over the semi-arid regions covered by grass.
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http://dx.doi.org/10.1016/j.scitotenv.2022.157890DOI Listing
August 2022

Synergetic Insulation and Induction Effects Selectively Optimize Multiresonance Thermally Activated Delayed Fluorescence.

Research (Wash D C) 2022 2;2022:9838120. Epub 2022 Jun 2.

Key Laboratory of Functional Inorganic Material Chemistry, Ministry of Education, School of Chemistry and Materials, Heilongjiang University, China.

Multiresonance (MR) emitters featuring narrowband emissions and theoretically 100% exciton harvesting are great potential for organic light-emitting diode (OLED) applications. However, how to functionalize MR molecules without scarifying emission color purity is still a key challenge. Herein, we report a feasible strategy for selective optimization of MR molecules, which is demonstrated by a blue MR emitter substituted with a diphenylphosphine oxide (DPPO) group. Compared to its DPPO-free parent molecule, preserves narrowband feature with full widths at half maximum (FWHM) values of 28 nm in film and 32 nm in OLEDs and achieves 40% increased photoluminescence (92%) and electroluminescence quantum efficiencies (28%). It is showed that insulation effect of P=O effectively confines the singlet excited state on MR core to keep emission color purity, and its induction effect enhances singlet radiation and triplet-to-singlet conversion. This synergism for selective optimization is based on rational linkage between MR core and functional groups.
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http://dx.doi.org/10.34133/2022/9838120DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9275084PMC
June 2022

Engineering Ag nanoparticles and amorphous MoO on three dimensional N-doped carbon networks for enhanced lithium storage performance.

J Colloid Interface Sci 2022 Jul 30;628(Pt A):308-317. Epub 2022 Jul 30.

School of Chemistry & Materials Engineering, Fuyang Normal University, Fuyang 236037, PR China. Electronic address:

Molybdenum-based oxides have been widely investigated as promising material for lithium ion batteries owing to their unique physical and chemical properties as well as the large specific capacities. However, the fast capacity fading and poor cyclability originated from the large volume expansion and the sluggish electrode kinetics still inhibit their practical application. Herein, Ag nanoparticles combined with amorphous MoO-in-plane nanoconfined on three dimensional N-doped porous carbon networks (3DNC) are designed and synthesized through salt-template strategy accompanied by annealing treatment and hydrothermal method (3DNC-MoO-Ag). The synergistic effect of Ag nanoparticles and amorphous MoO can inhibit the "dead volume" and aggregation of the electrode, accommodate the volume change, accelerate the diffusion kinetics during the lithium ion intercalation and de-intercalation processes. As a result, the designed 3DNC-MoO-Ag delivers prominent cycling stability (834 mAh g after 100 cycles at 200 mA g) and excellent rate performance (419 mAh g after 70 cycles at 5000 mA g). Even at 5000 mA g, a specific capacity as high as 369 mAh g can be achieved after 500 cycles.
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http://dx.doi.org/10.1016/j.jcis.2022.07.171DOI Listing
July 2022

Inhibition of osteoclastogenesis by interleukin-33 administration in the periodontal ligament under mechanical loading.

J Periodontal Res 2022 Aug 5. Epub 2022 Aug 5.

State Key Laboratory of Oral Diseases & National Clinical Research Center for Oral Diseases, Department of Orthodontics, West China School & Hospital of Stomatology, Sichuan University, Chengdu, China.

Background And Objectives: The molecular mechanisms mediating external root resorption are poorly understood. Interleukin-33 (IL-33) expression increased remarkably in the periodontal ligament (PDL) under orthodontic loading. The IL-33-driven responses are delicately cell type- and tissue context-dependent. It is unknown how IL-33 act on osteoclastogenesis in the context of root surface. This study aimed to investigate the effect of IL-33 on osteoclastogenesis in the PDL under mechanical loading.

Materials And Methods: C57BL/6J mice were treated with injections of phosphate buffer saline (PBS) or recombinant mouse IL-33 (rmIL-33, 6 μl, 30 μg/ml), and subjected to models of orthodontic tooth movement. Tartrated resistant acid phosphates (TRAP)-positive cells and IL-33 expressions were examined in the PDL. IL-33 release from human PDL cells (hPDLCs) was detected by ELISA. Cementoblast-like (OCCM-30) cells were cultured in the presence of rmIL-33 to examine the release of osteoclast-regulatory proteins. The effects of rmIL-33 on osteoclastogenesis were examined in vitro in cultures of bone marrow macrophages (BMMs) and in BMMs-OCCM-30 cocultures. Expressions of osteoclast-specific or -related genes and proteins were investigated in BMMs-OCCM-30 cocultures treated with or without rmIL-33, in the presence or absence of granulocyte-macrophage colony-stimulating factor (GM-CSF) neutralizing antibody.

Results: Interleukin-33 expressions were upregulated in the PDL under orthodontic loading. Static compressive force enhanced expression and release of IL-33 from hPDLCs. Administration of rmIL-33 resulted in reduced number of TRAP-positive cells in the PDL, and inhibited osteoclast differentiation from BMMs in vitro. OCCM-30 cells had varied osteoprotegerin (OPG) / receptor activator for nuclear factor-κB ligand (RANKL) secretion and increased release of GM-CSF under rmIL-33 stimulation. Treatment with rmIL-33 in BMMs-OCCM-30 cocultures resulted in inhibited differentiation and decreased activity of osteoclasts, and these effects were partially reversed by GM-CSF neutralizing antibody.

Conclusions: Interleukin-33 inhibits osteoclastogenesis in the PDL under orthodontic loading. The anti-osteoclastogenic effects were mediated partly by directly affecting osteoclast precursors and partly by cementoblast-mediated release of GM-CSF.
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http://dx.doi.org/10.1111/jre.13039DOI Listing
August 2022

Downregulation of TRIM33 Promotes Survival and Epithelial-Mesenchymal Transition in Gastric Cancer.

Technol Cancer Res Treat 2022 Jan-Dec;21:15330338221114505

Department of Pharmaceutical Sciences, Biomanufacturing Research Institute and Technological Enterprise (BRITE), North Carolina Central University, Durham, NC, USA.

Among all malignancies worldwide, gastric cancer is the fifth most common cancer with the third highest mortality rate. One of the main reasons for the low survival rate is the recurrence and metastasis that occurs in many patients after surgery. Numerous studies have shown that abnormal TRIM33 expression is associated with the progression of malignant tumors. TRIM33 can function either as a tumor suppressor or tumor promoter in different cancers. Our data showed that TRIM33 was highly expressed in stomach cancer, and in human gastric cancer tissues, low expression of TRIM33 was associated with poor prognosis in patients with gastric cancer. To clarify the function of TRIM33 in survival and epithelial-mesenchymal transition in gastric cancer cells, we investigated the effect of TRIM33 knockdown in several gastric cancer cell lines. Downregulation of TRIM33 in BGC-823 and SGC-7901 cells enhanced the proliferation, colony formation, and migratory ability of these gastric cancer cells. It also promoted epithelial-mesenchymal transition; transfection of cells with siRNA targeting TRIM33 led to the upregulation of vimentin and N-Cadherin expression, and downregulation of E-Cadherin expression. Meanwhile, the transforming growth factor beta pathway was activated: levels of transforming growth factor beta were elevated and the expressions of p-Smad2, Smad2, Smad3, and Smad4 were activated. To confirm the role of TRIM33 in vivo, a xenograft model was established in nude mice. Immunohistochemical analysis identified that the protein levels of TRIM33, p-Smad2, Smad2, Smad3, Smad4, vimentin, and N-Cadherin were increased, and E-Cadherin levels were decreased, in xenograft tumors from the si-TRIM33 group. Taken together, these results suggest that TRIM33 may be a potential marker for the diagnosis and prognosis of gastric cancer. Furthermore, it may also serve as a novel target for gastric cancer treatment.
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http://dx.doi.org/10.1177/15330338221114505DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9358585PMC
August 2022

The Establishment of a Mouse Model for Degenerative Kyphoscoliosis Based on Senescence-Accelerated Mouse Prone 8.

Oxid Med Cell Longev 2022 20;2022:7378403. Epub 2022 Jul 20.

Division of Spine Surgery, Department of Orthopedic Surgery, Nanjing Drum Tower Hospital, The Affiliated Hospital of Nanjing University Medical School, China.

Objective: Degenerative kyphoscoliosis (DKS) is a complex spinal deformity associated with degeneration of bones, muscles, discs, and facet joints. The aim of this study was to establish an animal model of degenerative scoliosis that recapitulates key pathological features of DKS and to validate the degenerative changes in senescence-accelerated mouse prone 8 (SAMP8) mice.

Methods: Thirty male mice were divided into 2 groups: 10 bipedal C57BL/6J mice were used as the control group, and 20 bipedal SAMP8 mice were used as the experimental group. Mice were bipedalized under general anesthesia. The incidence of scoliosis and bone quality was determined using radiographs and in vivo micro-CT images 4, 8, and 12 weeks after surgery, respectively. Histomorphological studies of muscle samples were performed after sacrifice at 12 weeks after surgery.

Results: On the 12th week, the incidence rates of kyphosis in C57BL/6J and SAMP8 groups were 50% and 100%, respectively. Overall, the incidence and angle of kyphosis were significantly higher in the bipedal SAMP8 group compared to the C57BL/6J group (44.7°± 6.2° vs. 84.3°± 10.3°, P<0.001). Based on 3D reconstruction of the entire spine, degeneration of the intervertebral disc was observed in bipedal SAMP8 mice, including the reduction of disc height and the formation of vertebral osteophytes. The bone volume ratio (BV/TV) was significantly suppressed in the bipedal SAMP8 group compared with the bipedal C57BL/6J group. In addition, HE staining and Mason staining of the paraspinal muscle tissue showed chronic inflammation and fibrosis in the muscles of the bipedal SAMP8 group.

Conclusions: The SAMP8 mouse model can be taken as a clinically relevant model of DKS, and accelerated aging of the musculoskeletal system promotes the development of kyphosis.
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http://dx.doi.org/10.1155/2022/7378403DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9329026PMC
August 2022

Does completion of radical hysterectomy improve oncological outcomes of women with clinical early-stage cervical cancer and intraoperative detection of nodal involvement?: protocol for a systematic review and meta-analysis.

BMJ Open 2022 07 29;12(7):e056848. Epub 2022 Jul 29.

Department of Obstetrics and Gynecology, Enshi Clinical College of Wuhan University, Enshi, Hubei, China.

Introduction: The management of women with clinical early-stage cervical cancer and lymph node involvement detected intraoperatively is heterogeneous and controversial. This paper presents the protocol of a systematic review and meta-analysis regarding the management of this specific population of patients. This proposed study aims to answer the question: does completion of radical hysterectomy improve the oncological outcomes of women with clinical early-stage cervical cancer and intraoperatively detected nodal involvement?

Methods And Analysis: This protocol is drafted following the Preferred Reporting Items for Systematic Reviews and Meta-Analyses Protocols guidelines, and the proposed study will be conducted in accordance with the standard guidelines of 'Preferred Reporting Items for Systematic Reviews and Meta-Analyses' and 'Meta-analysis of Observational Studies in Epidemiology reporting guideline'. Comprehensive literature searches will be performed in PubMed, Embase, Scopus, and Web of Science. The screening of the eligible studies, the extraction of data of interest, and the quality assessment of the included studies will all be independently performed by different members of our team. The primary outcome of this proposed study will be comparing the risk of recurrence or death from cervical cancer and the risk of all-cause death in patients with two different treatments (completion of radical hysterectomy or abandonment of radical hysterectomy); the secondary outcome of this proposed study will be comparing the risk of the grade 3/4 toxicities associated with the two types of management. Given the clinical heterogeneity among the included studies, data on outcomes will be pooled by random-effects models. Heterogeneity will be evaluated using the statistic. The risk of bias for the included studies will be evaluated using the Newcastle-Ottawa Scale or the Cochrane collaboration's tool. The grade of evidence will be evaluated by two independent members of our team using the Grading of Recommendations, Assessment, Development and Evaluations approach.

Ethics And Dissemination: Ethical approval is not required because there will no primary data collected. The findings of this proposed study will be published in an international peer-reviewed journal.

Prospero Registration Number: CRD42021273527.
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http://dx.doi.org/10.1136/bmjopen-2021-056848DOI Listing
July 2022

Preparation of resistant glucan by high pressure processing (HPP) and enzymatic methods increases indigestibility.

Int J Biol Macromol 2022 Jul 26;219:150-158. Epub 2022 Jul 26.

Qilu University of Technology (Shandong Academy of Sciences), Shandong Food Ferment Industry Research & Design Institute, Jinan 250000, China. Electronic address:

This study describes a new method for producing high-quality resistant glucan by characterizing the structural mechanism of indigestibility. The structures and properties of resistant glucans were characterized before and after in vitro simulated digestion. The results demonstrated that high-pressure processing (HPP) led to the complete disappearance of crystal peaks and increased the efficiency of the two enzymes (α-amylase and transglucosidase). Moreover, α-1,6 and β-linkages were abundant in the connecting parts of the long and branching chains in the resulting resistant glucans, thus hindering the ability of digestive enzymes to hydrolyze short chains with a degree of polymerization (DP) ≤ 6. In addition, transglucosidase activity led to a higher proportion of short chains (DP 3-6), further promoting indigestibility. We demonstrated that, without rectification and decolorization, the dietary fiber content was >75 %, and the degree of branching increased to 50.9 %, indicating higher indigestibility than that resistant glucans produced by traditional methods.
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http://dx.doi.org/10.1016/j.ijbiomac.2022.07.173DOI Listing
July 2022

Prognostic signature for hepatocellular carcinoma based on 4 pyroptosis-related genes.

BMC Med Genomics 2022 07 28;15(1):166. Epub 2022 Jul 28.

Department of Infectious Diseases and Key Laboratory of Liver Regenerative Medicine of Jiangxi Province, The First Affiliated Hospital of Nanchang University, Nanchang, 330006, Jiangxi, China.

Background: Hepatocellular carcinoma (HCC) is a cancer with a poor prognosis. Many recent studies have suggested that pyroptosis is important in tumour progression. However, the role of pyroptosis-related genes (PRGs) in HCC remains unclear.

Materials And Methods: We identified differentially expressed PRGs in tumours versus normal tissues. Through univariate, LASSO, and multivariate Cox regression analyses, a prognostic PRG signature was established. The signature effectiveness was evaluated by time-dependent receiver operating characteristic (t-ROC) curve and Kaplan-Meier (KM) survival analysis. The signature was validated in the ICGC (LIRI-JP) cohort. In addition, single-sample gene enrichment analysis (ssGSEA) showed the infiltration of major immune cell types and the activity of common immune pathways in different subgroups.

Results: Twenty-nine pyroptosis-related DEGs from The Cancer Genome Atlas-Liver Hepatocellular Carcinoma (TCGA-LIHC) dataset were detected, and four genes (CTSV, CXCL8, MKI67 and PRF1) among them were selected to construct a prognostic signature. Then, the patients were divided into high- and low-risk groups. The pyroptosis-related signature was significantly associated with overall survival (OS). In addition, the patients in the high-risk group had lower levels of immune infiltration.

Conclusion: The prognostic signature for HCC based on 4 pyroptosis-related genes has reliable prognostic and predictive value for HCC patients.
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http://dx.doi.org/10.1186/s12920-022-01322-9DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9336086PMC
July 2022

Sox11b regulates the migration and fate determination of Müller glia-derived progenitors during retina regeneration in zebrafish.

Neural Regen Res 2023 Feb;18(2):445-450

Key Lab of Neuroregeneration of Jiangsu and Ministry of Education, Co-innovation Center of Neuroregeneration, NMPA Key Laboratory for Research and Evaluation of Tissue Engineering Technology Products, Nantong University, Nantong, Jiangsu Province, China.

The transcription factor Sox11 plays important roles in retinal neurogenesis during vertebrate eye development. However, its function in retina regeneration remains elusive. Here we report that Sox11b, a zebrafish Sox11 homolog, regulates the migration and fate determination of Müller glia-derived progenitors (MGPCs) in an adult zebrafish model of mechanical retinal injury. Following a stab injury, the expression of Sox11b was induced in proliferating MGPCs in the retina. Sox11b knockdown did not affect MGPC formation at 4 days post-injury, although the nuclear morphology and subsequent radial migration of MGPCs were altered. At 7 days post-injury, Sox11b knockdown resulted in an increased proportion of MGPCs in the inner retina and a decreased proportion of MGPCs in the outer nuclear layer, compared with controls. Furthermore, Sox11b knockdown led to reduced photoreceptor regeneration, while it increased the numbers of newborn amacrines and retinal ganglion cells. Finally, quantitative polymerase chain reaction analysis revealed that Sox11b regulated the expression of Notch signaling components in the retina, and Notch inhibition partially recapitulated the Sox11b knockdown phenotype, indicating that Notch signaling functions downstream of Sox11b. Our findings imply that Sox11b plays key roles in MGPC migration and fate determination during retina regeneration in zebrafish, which may have critical implications for future explorations of retinal repair in mammals.
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http://dx.doi.org/10.4103/1673-5374.346550DOI Listing
February 2023

Overview of Piperine: Bioactivities, Total Synthesis, Structural Modification, and Structure-Activity Relationships.

Mini Rev Med Chem 2022 Jul 26. Epub 2022 Jul 26.

School of Marine Sciences, Ningbo University, Ningbo 315211, Zhejiang Province, P. R. China.

Natural products are an invaluable source for the discovery of drug and pesticide candidates. Piperine, a simple and pungent alkaloid, is isolated from several plants of Piperaceae. Piperine and its derivatives displayed a wide range of biological properties, such as antitumor activity, anti-inflammatory activity, antioxidant activity, neuroprotective activity, insecticidal activity, etc. In recent years, lots of works focused on the biological activities, mechanisms of action, total synthesis, and structural modifications of piperine and its derivatives have been conducted. To the best of our knowledge, however, few review articles related to the biological activities, mechanisms of action, total synthesis, and structural modifications of piperine and its derivatives have been reported up to date. Therefore, this review summarizes the research advances (from 2014 to 2020) of piperine and its derivatives regarding bioactivity, mechanisms of action, total synthesis, and structural modifications. Meanwhile, the structure-activity relationships of piperine and its derivatives are also discussed.
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http://dx.doi.org/10.2174/1389557522666220726121012DOI Listing
July 2022

The Relationship Between the Fibrinogen to Albumin Ratio and Early Outcomes in Patients with Acute Pontine Infarction.

Clin Appl Thromb Hemost 2022 Jan-Dec;28:10760296211067260

The Affiliated Fuyang People's Hospital of Anhui Medical University, Fuyang, China.

Background And Purpose: Previous studies have indicated that fibrinogen and low serum albumin levels are associated with poor outcomes of acute ischemic stroke. The role of the fibrinogen-to-albumin ratio (FAR) as a novel inflammatory and thrombotic biomarker in acute ischemic stroke is unclear. This study aims to investigate the relationship between the FAR and 3-month outcomes of acute pontine infarction. : Patients with acute pontine infarction were consecutively included. All patients were followed up at 3 months after onset, and the 3-month outcome was evaluated using modified Rankin Scale (mRS) scores. A score of 0 to 2 was defined as a good outcome, and a score ≥ 3 was defined as a poor outcome. Receiver operating curve (ROC) analysis was used to calculate the optimal cutoff values for patients with acute pontine infarction. Then, a binary logistic regression model was used to evaluate the risk factors for a poor outcome after acute pontine infarction. A total of 264 patients with acute pontine infarction were included. Eighty (30.3%) patients were included in the poor outcome group. The optimal cutoff value of the FAR for predicting the 3-month outcome of acute pontine infarction was 8.199. The FAR was independently associated with a poor outcome at 3 months in patients with acute pontine infarction (odds ratio [OR] = 1.293, 95% confidence interval [CI]: 1.150-1.453). We found that a high FAR predicted poor 3-month outcomes in patients with acute pontine infarction.
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http://dx.doi.org/10.1177/10760296211067260DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8733351PMC
July 2022

Investigating the Role of Dahuang in Hepatoma Treatment Using Network Pharmacology, Molecular Docking, and Survival Analysis.

Biomed Res Int 2022 15;2022:5975223. Epub 2022 Jul 15.

Department of Nephrology, Mianyang Central Hospital, School of Medicine, University of Electronic Science and Technology of China, Mianyang 621000, China.

Hepatoma is one of the most common malignant tumors. The incidence rate is high in developing countries, and China has the most significant number of cases. Dahuang is a classic traditional antitumor drug commonly used in China and has also been applied to treat hepatoma. However, the potential mechanism of Dahuang in treating hepatoma is not clear. Therefore, this study is aimed at elucidating the possible molecular mechanism and key targets of Dahuang using methods of network pharmacology, molecular docking, and survival analysis. Firstly, the active ingredients and key targets of Dahuang were analyzed through public databases, and then the drug-ingredient-target-disease network diagram of Dahuang against hepatoma was constructed. Five main active components and five core targets were determined according to the enrichment degree. Enrichment analysis demonstrated that Dahuang treated hepatoma through the multiple pathways in cancer. Additionally, molecular docking predicted that aloe-emodin and PIK3CG depicted the best binding energy. Survival analysis indicated that a high/ESR1 gene expression had a relatively good prognosis for patients with hepatoma ( < 0.05). In conclusion, the current study results demonstrated that Dahuang could treat hepatoma through a variety of active ingredients, targets, and multiantitumor pathways. Moreover, it effectively improved the prognosis of hepatoma patients. ESR1 is the potential key gene that is beneficial for the survival of hepatoma patients. Also, aloe-emodin and beta-sitosterol are the two main active crucial ingredients for hepatoma treatment. The study also provided some functional bases and references for the development of new drugs, target mining, and experimental animal research of hepatoma in the future.
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http://dx.doi.org/10.1155/2022/5975223DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9307382PMC
July 2022

Genetic spectrum of Chinese children with cystic fibrosis: comprehensive data analysis from the main referral centre in China.

J Med Genet 2022 Jul 20. Epub 2022 Jul 20.

Department II of Respiratory Medicine, National Clinical Research Center for Respiratory Diseases, Beijing Children's Hospital, Capital Medical University, National Center for Children's Health, Beijing, People's Republic of China

Background And Objectives: Cystic fibrosis (CF) is a heterogeneous disease with a diverse genetic spectrum among populations. Few patients with CF of Chinese origin have been reported worldwide. The objective of this study is to characterise the genotypic features of CF in Chinese children.

Methods: We recruited and characterised the genetic manifestations of 103 Chinese children with CF in Beijing Children's Hospital from 2010 to 2022. Whole-exome sequencing were performed to define the genotypes. Meanwhile, other 99 genetically confirmed patients with Chinese origin described in 45 references were also summarised.

Results: 158 different variants including 23 novel observations were identified after sequencing. The majority of variants (82.3%) in Chinese have been observed only once or twice. 43.7% of the variants were only identified in patients of Chinese origin. The c.2909G>A(p.Gly970Asp), c.1766+5G>T and c.1657C>T(p.Arg553X) were the most frequent variants among Chinese patients, with allele frequency of 12.1%, 5.4% and 3.6%, respectively. The first two variants both showed significant Chinese ethnic tendency, while the latter one most likely came from Europeans for historical reasons. They also demonstrated significant differences in geographical distribution. c.1521_1523delCTT(p.F508del) was rarely observed in patients of pure Chinese origin, with an allele frequency of 1.8%. Two de novo variants (c.960dupA[p.Ser321IlefsX43] and c.2491-2A>G) and two deep-intronic variants (c.3718-2477C>T and c.3874-4522A>G) were identified, which were also quite rare among Chinese.

Conclusions: The genetic spectrum of CF in Chinese is unique and quite different from that observed in Caucasians. The geographical distributions of the most frequent variants were reported for the first time.
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http://dx.doi.org/10.1136/jmg-2022-108501DOI Listing
July 2022

Construction, Pesticidal Activities, Control Effects, and Detoxification Enzyme Activities of Osthole Ester/Amide Derivatives.

J Agric Food Chem 2022 Aug 20;70(30):9337-9345. Epub 2022 Jul 20.

College of Plant Protection, Northwest A&F University, Yangling, Shaanxi 712100, China.

Pesticide research and development has entered an era of safety, efficiency, and environmental friendliness. Discovery of effective active products directly or indirectly from plant secondary metabolites as pesticide candidates has been one of the current research focuses. Herein, two series of new ester and amide derivatives were prepared by structural modifications of a natural coumarin-type product osthole at its C-4' position. Their structures were characterized by IR, mp, H NMR, and HRMS. Confirmation of steric configuration of seven compounds was based on single-crystal analysis. Against Boisduval (Acari: Tetranychidae), (2')-3'-ethoxycarbonylosthole () and (2')-3'-()hexyloxycarbonylosthole () exhibited 3.2 and 3.1 times acaricidal activity of osthole, and particularly, they also showed 2.4 and 2.2 times control efficiency on the 5th day of osthole. Against Van der Goot (Homoptera: Aphididae), (2')-3'-(-CF)benzyloxycarbonylosthole (), (2')-3'-benzylaminocarbonylosthole (), and (2')-3'-phenylethylaminocarbonylosthole () showed 1.9-2.1-fold aphicidal activity of osthole. Furthermore, the changes in two detoxification enzyme [carboxylesterase (CarE) and glutathione -transferase (GST)] activities over time in treated were investigated. These results can pave the foundation for future design and preparation of osthole derivatives as botanical agrochemicals.
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http://dx.doi.org/10.1021/acs.jafc.2c02534DOI Listing
August 2022

Combination effect of azithromycin with TCM preparation Xiyanping injection against Klebsiella pneumoniae infection in rats.

Phytomedicine 2022 Sep 10;104:154332. Epub 2022 Jul 10.

School of Pharmacy, Collaborative Innovation Center of Advanced Drug Delivery System and Biotech Drugs in Universities of Shandong, Key Laboratory of Molecular Pharmacology and Drug Evaluation, Yantai University, Yantai 264005, China. Electronic address:

Background: Klebsiella pneumoniae is known as one of the most principal opportunistic human pathogens. Although antibiotics such as the first-line agent azithromycin (AZM) usually are efficient for the treatment of K. pneumonia-related infections, growing threat from antibiotic resistance has become a major challenge. Various preparations based on traditional Chinese medicine (TCM) clinical experience have been developed to help combat such a global public health threat, including Xiyanping injection (XYP) that is made from the natural product andrographolide with potent heat-clearing and toxin-resolving functions.

Purpose: The present study aimed to demonstrate the therapeutic potential, as well as the action of mechanism of AZM in combination with XYP against K. pneumonia infection in rats.

Methods: Pneumonia model of K. pneumoniae infection in rats was established and subjected to various treatments. The lung histopathological lesions were evaluated. ELISA and Griess techniques were used to determine the level of crucial cytokines. The protein expressions of MAPKs and NF-κB pathways were analyzed by Western blotting.

Results: The combination in vivo could significantly inhibit the proliferation of K. pneumoniae in lung, improve the pathological changes of lung and reduce inflammatory factors in lung homogenate and bronchoalveolar lavage fluid, mainly by inactivating MAPKs and NF-κB signaling pathways. Combination therapy caused one-fold increase in apparent distribution volume of AZM in rats after multiple dosing, along with a significant increase of AZM level in lungs but obvious decrease in livers.

Conclusion: The combination therapy of AZM and XYP showed increased antibacterial and anti-inflammatory properties, indicating that it might be used to treat K. pneumoniae infection.
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http://dx.doi.org/10.1016/j.phymed.2022.154332DOI Listing
September 2022

Avoiding Unnecessary Systematic Biopsy in Clinically Significant Prostate Cancer: Comparison Between MRI-Based Radiomics Model and PI-RADS Category.

J Magn Reson Imaging 2022 Jul 19. Epub 2022 Jul 19.

Department of Radiology, West China Hospital, Sichuan University, Chengdu, China.

Background: MRI-targeted biopsy (MRTB) improves the clinically significant prostate cancer (csPCa) detection rate with fewer biopsy cores in men with suspected PCa. However, whether concurrent systematic biopsy (SB) can be avoided in patients undergoing MRTB remains unclear.

Purpose: To evaluate the potential value of MRI-based radiomics models in avoiding unnecessary SB in biopsy-naïve patients.

Study Type: Retrospective.

Population: A total of 226 patients (mean age 66.6 ± 9.02 years) with suspicion of PCa (PI-RADS score ≥ 3) and received combined cognitive MRTB with SB were retrospectively recruited and randomly divided into training (N = 180) and test (N = 46) cohorts at an 8:2 ratio.

Field Strength/sequence: A 3.0 T, biparametric MRI (bpMRI) including T2-weighted imaging (T2WI) and apparent diffusion coefficient (ADC) map.

Assessment: The whole prostate gland (PG) and the index lesion (IL) were delineated. Three radiomics models of bpMRI , bpMRI , and bpMRI were constructed, respectively, and the performance of each radiomics model was compared with that of PI-RADS assessment.

Statistical Tests: The least absolute shrinkage and selection operator (LASSO) regression method was used to select texture features. The area under the curve (AUC) and decision curve analysis were used to estimate the models.

Results: The bpMRI radiomics model exhibited good discrimination, calibration, and net benefits, which would reduce the SB biopsy in 71.2% and 71.4% of men with PI-RADS ≥ 5 lesions in the training and test cohorts, respectively.

Data Conclusion: A bpMRI radiomics model may outperform PI-RADS category in help reducing unnecessary SB in biopsy-naïve patients.

Evidence Level: 3 TECHNICAL EFFICACY: Stage 6.
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http://dx.doi.org/10.1002/jmri.28333DOI Listing
July 2022

SCG2: A Prognostic Marker That Pinpoints Chemotherapy and Immunotherapy in Colorectal Cancer.

Front Immunol 2022 1;13:873871. Epub 2022 Jul 1.

Department of Interventional Radiology, The First Affiliated Hospital of Zhengzhou University, Zhengzhou, China.

Background: Fluorouracil (FU)-based chemotherapy regimens are indispensable in the comprehensive treatment of colorectal cancer (CRC). However, the heterogeneity of treated individuals and the severe adverse effects of chemotherapy results in limited overall benefit.

Methods: Firstly, Weighted gene co-expression network analysis (WGCNA) identified modules tightly associated with chemotherapy response. Then, the in-house cohort and prognostic cohorts from TCGA and GEO were subjected to Cox proportional hazards model and survival analysis to ascertain the predictable function of SCG2 on the prognosis of CRC patients. Finally, we performed experiments, functional analysis, somatic mutation, and copy number variation research to explore the biological characteristics of SCG2.

Results: We identified red and green as the modules most associated with chemotherapy response, in which SCG2 was considered a risky factor with higher expression predicting poorer prognosis. SCG2 expression in the APC non-mutation group was remarkably higher than in the mutation group. The mutation frequencies of amplified genes differed significantly between different SCG2 expression subgroups. Besides, CRC cell lines with SCG2 knockdown have reduced invasive, proliferative, and proliferative capacity. We discovered that the SCG2 high expression subgroup was the immune hot type and considered more suitable for immunotherapy.

Conclusion: This study demonstrates the clinical significance and biological characteristics of SCG2, which could serve as a promising biomarker to identify patients who may benefit from chemotherapy and immunotherapy.
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http://dx.doi.org/10.3389/fimmu.2022.873871DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9283651PMC
July 2022

G-wire-based self-quenched fluorescence probe combining with target-activated isothermal cascade amplification for ultrasensitive microRNA detection.

Spectrochim Acta A Mol Biomol Spectrosc 2022 Nov 9;281:121605. Epub 2022 Jul 9.

Huzhou Key Laboratory of Medical and Environmental Applications Technologies, School of Life Sciences, Huzhou University, Zhejiang 313000, PR China.

Herein, we reported the G-wire-based self-quenched fluorescence probe and its application in ultrasensitive microRNA (miRNA) detection by combining with target-activated isothermal cascade amplification. The terminal-single-fluorescein (FAM)-labeled G-rich oligonucletides self-assembled into G-wire nanostructures (G-wires) with K and Mg. Thereafter, the G-wires brought terminal-labeled FAM into close proximity, as a result, the self-quenched signal probe formed. Besides, when there was the target miRNA, target-activated isothermal cascade amplification converted miRNA into the copious trigger DNA. After hybridization between trigger DNA and the self-quenched probe, the G-wires were splited and forced the apart of proximate FAM, and then the self-quenched probe displayed an "on" mechanism. Therefore, the approach gave a limit of detection (LOM) of 0.82 aM to miRNA-21 and could be implemented within a wide linear range of 2 aM to 2 nM. This approach was able to distinguish the single-mismatched miRNA-21, which was selective and sensitive in detecting human spiked serum samples.
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http://dx.doi.org/10.1016/j.saa.2022.121605DOI Listing
November 2022

Functional brain changes in Parkinson's disease: a whole brain ALE study.

Neurol Sci 2022 Jul 15. Epub 2022 Jul 15.

Department of Neurology, Affiliated ZhongDa Hospital, School of Medicine, Southeast University, No. 87 Dingjiaqiao Road, Nanjing, Jiangsu, 210009, China.

Background: Resting-state functional magnetic resonance imaging (rs-fMRI) was widely used as an effective tool in the diagnosis of neurodegenerative diseases. However, prior rs-fMRI studies reported inconsistent results for comparison between Parkinson's disease (PD) and healthy controls (HC).

Methods: We searched studies published before December 2021 in databases (PubMed, Web of Science, and Google Scholar). An activation likelihood estimation (ALE) meta-analysis was made for functional changes in PD.

Results: The study finally included 25 studies (including 973 PD patients and 766 HC). PD patients showed reduced amplitude of low frequency fluctuations (ALFF) in the left superior temporal gyrus (STG), the left superior frontal gyrus (SFG), the left medial frontal gyrus (MFG), the left precuneus (PCUN), and the right lentiform nucleus, compared to HC. PD patients showed increased ALFF in the right SFG, the left superior parietal lobule (SPL), the left STG, the right fusiform gyrus, the left inferior temporal gyrus (ITG), and the right parahippocampal gyrus (PHG), compared to HC. PD patients showed reduced regional homogeneity (ReHo) in the right declive, the right MFG, the left culmen, and the left thalamus, compared to HC. PD patients showed increased ReHo in the right SFG, compared to HC. Additionally, PD patients showed reduced functional connectivity (FC) in the right posterior cingulate (PCG), compared to HC.

Conclusions: The present ALE analysis has confirmed functional deficits in motor-, emotion-, and cognition-related regions in PD. Deficits in these regions in rs-fMRI studies could play a role in early diagnosis of PD.
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http://dx.doi.org/10.1007/s10072-022-06272-9DOI Listing
July 2022

Benchmarking Single-Cell mRNA-Sequencing Technologies Uncovers Differences in Sensitivity and Reproducibility in Cell Types With Low RNA Content.

J Biomol Tech 2021 12;32(4)

Takara Bio USA, Inc., San Jose, California, USA.

Single-cell RNA sequencing (scRNA-seq) has the ability to classify each cell and determine the transcriptomic profile of specific cell types and cells of a given disease state; however, sensitivity of the gene count for each cell can be a critical component to the success of a single-cell study. The recently introduced SMART-Seq Single Cell PLUS Kit (SSsc PLUS) claims to provide higher sensitivity and reproducibility versus popular methods for the sequencing analysis of single cells. Here, the cDNA-generation component of the kit, SMART-Seq Single Cell Kit (SSsc), was compared with the popular homebrew protocol, Smart-seq2, and its update, Smart-seq3. The SMART-Seq Library Prep Kit from SSsc PLUS was benchmarked against a commonly used scRNA-seq library preparation method, Illumina Nextera XT. Finally, the SSsc chemistry was tested in both full and fractional volumes on 2 popular liquid-handler devices to investigate whether the high sensitivity was maintained in miniaturization. We demonstrate that SSsc PLUS outperforms these other full-length methods in convenience, sensitivity, gene identification, and reproducibility while also offering full compatibility with automation platforms.
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http://dx.doi.org/10.7171/3fc1f5fe.dbeabb2aDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9258451PMC
December 2021

KDM4 inhibitor SD49-7 attenuates leukemia stem cell KDM4A/MDM2/p21 axis.

Theranostics 2022 21;12(11):4922-4934. Epub 2022 Jun 21.

State Key Laboratory of Experimental Hematology, National Clinical Research Center for Blood Diseases, Haihe Laboratory of Cell Ecosystem, PUMC Department of Stem Cell and Regenerative Medicine, CAMS Key Laboratory of Gene Therapy for Blood Diseases, Institute of Hematology & Blood Diseases Hospital, Chinese Academy of Medical Sciences & Peking Union Medical College, Tianjin 300020, China.

Traditional treatments for leukemia fail to address stem cell drug resistance characterized by epigenetic mediators such as histone lysine-specific demethylase 4 (KDM4). The KDM4 family, which acts as epigenetic regulators inducing histone demethylation during the development and progression of leukemia, lacks specific molecular inhibitors. The KDM4 inhibitor, SD49-7, was synthesized and purified based on acyl hydrazone Schiff base. The interaction between SD49-7 and KDM4s was monitored by surface plasma resonance (SPR). and biological function experiments were performed to analyze apoptosis, colony-formation, proliferation, differentiation, and cell cycle in cell sub-lines and mice. Molecular mechanisms were demonstrated by RNA-seq, ChIP-seq, RT-qPCR and Western blotting. We found significantly high expression levels in several human leukemia subtypes. The knockdown of KDM4s inhibited leukemogenesis in the MLL-AF9 leukemia mouse model but did not affect the survival of normal human hematopoietic cells. We identified SD49-7 as a selective KDM4 inhibitor that impaired the progression of leukemia stem cells (LSCs) . SD49-7 suppressed leukemia development in the mouse model and patient-derived xenograft model of leukemia. Depletion of KDM4s activated the apoptosis signaling pathway by suppressing expression modulating H3K9me3 levels on the promoter region. Our study demonstrates a unique KDM4 inhibitor for LSCs to overcome the resistance to traditional treatment and offers KDM4 inhibition as a promising strategy for resistant leukemia therapy.
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http://dx.doi.org/10.7150/thno.71460DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9274755PMC
June 2022

Downregulation of lncRNA ASMTL-AS1 in Epithelial Ovarian Cancer Correlates with Worse Prognosis and Cancer Progression.

Horm Metab Res 2022 Jul 14;54(7):481-488. Epub 2022 Jul 14.

Department of Obstetrics and Gynecology, The Central Hospital of Enshi Tujia and Miao Autonomous Prefecture, Enshi, China.

Given the characters of "Silent killer", epithelial ovarian cancer (EOC) usually suffered late diagnosis and poor prognosis. Therefore, this study aimed to explore the prognostic significance of ASMTL-AS1 in EOC and investigated the effect of lncRNA ASMTL-AS1 dysregulation on tumor cellular function. ASMTL-AS1 expression was analyzed in 133 EOC tissues and five kinds of cell lines by RT-qPCR. The expression of ASMTL-AS1 was tested for correlation with clinical data using the chi-square test and clinical follow-up using Kaplan-Meier method with log-rank test. Further, the prognostic parameters in predicting EOC overall survival were assessed by using multivariate Cox proportional hazards analysis. In vitro assays, including MTT assay and transwell assay, were conducted using EOC cell lines with overexpression of ASMTL-AS1. In tumorous tissues and cell lines, ASMTL-AS1 was lowly expressed compared with normal ones. This downregulation was associated with the advanced FIGO stage, positive ascites cytology, and lymph node. In particular, low levels of ASMTL-AS1 were revealed to have a high prognostic impact on EOC. ASMTL-AS1 overexpression strongly decreased cell proliferation, migration, and invasion in vitro partly by moderating miR-1228-3p. This study demonstrates a significant role for lowly expressed ASMTL-AS1 in EOC allowing for the prediction of prognosis for EOC. Considering that ASMTL-AS1 is strongly involved in cell growth and invasion, ASMTL-AS1 may be a promising marker for EOC prognosis and therapy.
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http://dx.doi.org/10.1055/a-1872-0546DOI Listing
July 2022

ALOX12: A Novel Insight in Bevacizumab Response, Immunotherapy Effect, and Prognosis of Colorectal Cancer.

Front Immunol 2022 27;13:910582. Epub 2022 Jun 27.

Department of Interventional Radiology, The First Affiliated Hospital of Zhengzhou University, Zhengzhou, China.

Colorectal cancer is a highly malignant cancer with poor prognosis and mortality rates. As the first biological agent approved for metastatic colorectal cancer (mCRC), bevacizumab was confirmed to exhibit good performance when combined with chemotherapy and immunotherapy. However, the efficacy of both bevacizumab and immunotherapy is highly heterogeneous across CRC patients with different stages. Thus, exploring a novel biomarker to comprehensively assess the prognosis and bevacizumab and immunotherapy response of CRC is of great significance. In our study, weighted gene co-expression network analysis (WGCNA) and the receiver operating characteristic (ROC) curves were employed to identify bevacizumab-related genes. After verification in four public cohorts and our internal cohort, ALOX12 was identified as a key gene related to bevacizumab response. Prognostic analysis and experiments further demonstrated that ALOX12 was closely associated with the prognosis, tumor proliferation, invasion, and metastasis. Multi-omics data analysis based on mutation and copy number variation (CNV) revealed that RYR3 drove the expression of ALOX12 and the deletion of 17p12 inhibited ALOX12 expression, respectively. Moreover, we interrogated the relationship between ALOX12 and immune cells and checkpoints. The results exhibited that high ALOX12 expression predicted a higher immune infiltration and better immunotherapy response, which was further validated in Tumor Immune Dysfunction and Exclusion (TIDE) and Subclass Mapping (SubMap) methods. Above all, our study provides a stable biomarker for clinical protocol optimization, prognostic assessment, precise treatment, and individualized treatment of CRC.
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http://dx.doi.org/10.3389/fimmu.2022.910582DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9271859PMC
July 2022

The Influence of Educational Psychology on Modern Art Design Entrepreneurship Education in Colleges.

Front Psychol 2022 27;13:843484. Epub 2022 Jun 27.

School of Business, Wenzhou University, Wenzhou, China.

The purpose of this study is to analyze the relationship between psychology and modern art design education in colleges, and to provide the basis for improving the quality of art education in colleges. Based on the relevant theory, the characteristics of educational psychology and art psychology and the correlation between them were analyzed from the angle of art education. According to the characteristics of college students' psychological development, the significance of art psychology-related courses was analyzed. Through the form of questionnaire, a total of 200 students (400 students in total) were randomly selected from Xi'an Academy of Fine Arts (professional art college) and Xi'an University of Science and Technology (comprehensive university), respectively. A total of 400 questionnaires were issued, and 382 valid questionnaires were recovered. The results show that almost all students in two colleges think pedagogy and educational psychology are the theoretical basis for art teachers, while less than one-third of students in Xi'an University of Science and Technology think that art psychology is the theoretical basis for art teachers. The difference between the students' aesthetic and life values in the two colleges is significant ( < 0.01). In the 15 directions of life values, there are significant differences in five directions: spirit-material ( < 0.05), enrichment-emptiness ( < 0.05), enthusiasm-apathy ( < 0.05), hope-despair ( < 0.01), and dedication-acceptance ( < 0.01). It shows that the psychological and values of college students are changeable, and the educators should pay attention to the education in the related fields of psychology. This exploration was conducted based on educational psychology, which is of great significance for improving the educational level of art psychology in colleges and enable students to form correct aesthetic standards and life values.
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http://dx.doi.org/10.3389/fpsyg.2022.843484DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9271969PMC
June 2022
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