Publications by authors named "Hui Wu"

1,300 Publications

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Agricultural water and land resources allocation considering carbon sink/source and water scarcity/degradation footprint.

Sci Total Environ 2021 Nov 30:152058. Epub 2021 Nov 30.

Center for Agricultural Water Research in China, China Agricultural University, Beijing 100083, China.

There is an urgent need for scientific management of agricultural water and land resources to cope with global warming and water shortages. Therefore, a stochastic multi-objective non-linear programming model was established under the society-economy-ecology framework in this study, which is capable of (1) considering the carbon sink function of farmland vegetation and the carbon emissions produced from the input of production materials; (2) dealing with the potential impact of agricultural water use on ecology by the indexes of water scarcity and degradation footprint (3) weighing the conflicts and contradictions among different developing targets with economic benefits, and water productivity as well as ecological considerations; (4) obtaining the planting structure, irrigation water allocation schemes and irrigation schedules. In order to verify the applicability and effectiveness of the model, it was applied to Huangyang Irrigation District in Shiyang River Basin, northwest China. After optimization, the net carbon sink increased by 2.55 × 10 t of which fertilizer contributed nearly 50% of carbon emission, while the water footprint reduced by 0.48 × 10 m. To analyze the impact of the different allocation schemes of water and land resources on the ecological, economic and social subsystems as well as their interaction, coupling coordination degree (CCD) models were introduced to evaluate status quo and the optimization results of different models. The results showed that, compared with the single-objective models and the status quo, the proposed model has improved the value of CCD from 0.428 to 0.674. The proposed model can promote the harmonious and sustainable development of agricultural production and is equally applicable to agricultural management systems in the regions with similar conditions.
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http://dx.doi.org/10.1016/j.scitotenv.2021.152058DOI Listing
November 2021

The transmission of drug-resistant strains of HIV in heterosexual populations based on genetic sequences.

PLoS One 2021 1;16(12):e0259023. Epub 2021 Dec 1.

Department of Mathematics, Shanghai University, Shanghai, China.

Background: China's National Free Antiretroviral Treatment Program (NFATP) has substantially reduced morbidity and HIV/AIDS incidence since 2003. However, HIV resistance to antiretroviral drugs (ARVs) has been a major challenge for the current treatment of HIV/AIDS in China.

Methods: In the current study, we established a nested dynamic model to predict the multi-drug resistance dynamics of HIV among the heterosexual population and evaluated the impact of intervention measures on the transmission of drug resistance. We obtained an effective reproductive number [Formula: see text] from each sub-model held at different stages of the dynamic model. Meanwhile, we applied Bayesian phylogenetic methods to infer the weighted average effective reproductive number [Formula: see text] from four HIV subtypes that sampled from 912 HIV-positive patients in China. It is an original and innovative method by fitting [Formula: see text] to [Formula: see text] by Markov Chain Monte Carlo (MCMC) to generate unknown parameters in [Formula: see text].

Results: By analyzing the HIV gene sequences, we inferred that the most recent common ancestor of CRF01AE, CRF07BC, CRF08BC, and CRFBC dated from 1994, 1990, 1993 and 1990, respectively. The weighted average effective reproductive number [Formula: see text] dropped from 1.95 in 1994 to 1.73 in 2018. Considering different interventions, we used a macro dynamic model to predict the trend of HIV resistance. The results show that the number of new infections and total drug resistance under the baseline parameter (S1) are 253,422 and 213,250 in 2025, respectively. Comparing with the numbers under the target treatment rate (S2), they were 219,717 and 236,890, respectively. However, under the ideal treatment target (S3, the treatment rate reaches 90% and the treatment success rate reaches 90%), the number of new infections shows a declining trend and will decrease to 46,559 by 2025. Compared with S1 and S2, the total number of resistance also decreased to 160,899 in 2025.

Conclusion: With the promotion of NFATP in China, HIV resistance to ARVs is inevitable. The strategy of increasing the treatment rate would not only ineffectively curb the epidemic, but also deteriorate drug resistance issue. Whereas, a combination of intervention strategies (the treatment rate reaches 90% and the treatment success rate reaches 90%) can greatly reduce both infection and drug resistance rate than applying one strategy alone.
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http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0259023PLOS
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8635345PMC
December 2021

Preoperative Predictors of Prolonged Hospital Stay in Accelerated Rehabilitation for Patients Undergoing Orthopedic Surgery.

Bioinorg Chem Appl 2021 19;2021:7832216. Epub 2021 Nov 19.

Department of Orthopedics, Hunan Provincial People's Hospital (The First-affiliated Hospital of Hunan Normal University), Changsha 410005, China.

In this study, we performed a retrospective and prospective study of preoperative predictors of the length of stay (LOS) in three groups of surgical patients and conducted a clinical retrospective study of the current research status of preoperative predictors of LOS prolongation in three groups of patients under ERAS (enhanced recovery after surgery) mode, such as patient characteristics and comorbidities. Information such as patients' exercise preferences, exercise time, frequency and duration, footwear, location of knee osteoarthritis, whether there is a past history of knee injury, and smoking and drinking history was collected, and the research data of 312 patients undergoing the three operations were analyzed by SPSS. Meniscal injury-knee arthroscopy sample included a total of 104 people. Surgical sample for anterior cruciate ligament reconstruction included a total of 100 subjects. Knee osteoarthritis-knee replacement surgery sample included 148 people who were divided into two groups in a ratio of 1 : 1: one group used Mailuo Shutong pills during hospitalization (intervention group) and the other group did not (control group). The research conclusions are as follows. . (1) Samples from different causes of injury showed significant differences for all injured sites. (2) Samples with different smoking and drinking histories all showed significant differences for the causes of injury. (3) Exercise hobby, exercise frequency, duration of each exercise and duration of exercise, and warm-up time before exercise all showed positive correlation. . (4) Samples from different causes of injury showed significant differences for all the injured sites. (5) Age has a significant negative influence on the wearing of shoes at ordinary times. (6) Exercise hobby: the warm-up time before exercise had a significant negative influence on the injured area. (7) Two groups of analysis items of exercise frequency, exercise duration and exercise duration, preexercise warm-up time, and exercise hobby were typically positively correlated. . (8) There was a significant difference of 0.01 between the hospitalization days of the intervention group and the control group ( < 0.01), and the hospitalization days of the intervention group were significantly lower than those of the control group. These results indicated that Mailuo Shutong pills were of great significance for the treatment of orthopedic patients during the operation period in that it could effectively shorten the hospital stay of all orthopedic patients and strengthen the accelerated rehabilitation. (9) There was a significant positive correlation between the history of knee joint surgery and the use time of Mailuo Shutong pills. (10) There was a markable positive correlation between occupation and sports hobbies, sports time, frequency and duration, and footwear. There was a significant negative correlation between occupation and preexercise warm-up. (11) Exercise time, frequency, and duration have significant positive influence on BMI.
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http://dx.doi.org/10.1155/2021/7832216DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8626200PMC
November 2021

Highly efficient scavenging of Cr(VI) by two-dimensional titanium carbide nanosheets: kinetics, isotherms and thermodynamics analysis.

Water Sci Technol 2021 Nov;84(9):2446-2456

College of Chemistry and Chemical Engineering, Shaoxing University, Shaoxing, Zhejiang 312000, China E-mail:

In this study, two-dimensional (2D) MXene material (TiCT) was employed to investigate its potentials toward the Cr(VI) removal in aqueous system by batch experiments. Characterization techniques such as SEM-EDS, HRTEM, XRD, FI-TR and XPS were used to analyze the structure and interaction of TiCT before and after Cr(VI) adsorption. The results indicated that the layered structure of TiCT had unique surface functional properties and abundant active sites, such as -OH, Ti-O, C = O, which exhibited high adsorption capacity for Cr(VI) removal. The Cr(VI) adsorption capacity by TiCT decreased with the increase of pH, and its maximum value can reach 169.8 mg/g at pH = 2.0. The adsorption kinetic was well-explained by a pseudo-second-order kinetic, indicating that chemical interaction played a dominant role in the adsorption of Cr(VI) on TiCT. Meanwhile, the isotherm data was calculated to conform to the Freundlich isotherm model. Thermodynamic analysis indicated that the adsorption process of Cr(VI) on TiCT was a spontaneous endothermic process. These experimental results revealed that TiCT had tremendous potential in heavy metals adsorption from aqueous solutions.
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http://dx.doi.org/10.2166/wst.2021.434DOI Listing
November 2021

Establishment of the reference intervals of lymphocyte subsets for healthy Chinese Han adults and its influencing factors.

Ann Transl Med 2021 Oct;9(19):1495

Department of Laboratory Medicine, Zhongshan Hospital, Fudan University, Shanghai, China.

Background: Cellular immune monitoring is becoming more critical in the clinic, but its application has not yet become sufficiently widespread. One reason may be the different reference intervals among clinical laboratories due to several factors. Percentage and number of lymphocyte subsets are standard indicators of cellular immune detection. The present study aimed to establish standardized reference intervals of lymphocyte subsets in the healthy Chinese Han adult population and examine such influencing factors as age, gender, region, and measurement instruments.

Methods: A total of 496 healthy Chinese Han people aged 18-59 years from 3 China Mainland regions (north, east, and south) were enrolled. The sample of each center was simultaneously examined by three flow cytometers (FACSCantoII, FACSLyric, and FACSCalibur). A single-platform flow cytometry-based absolute count technique was used to quantify the percentage and number of each lymphocyte subset. The flow cytometry results were analyzed by variance analysis and Z test to determine the influence of age, gender, and instruments on lymphocyte subsets.

Results: Multi-center, age-specific, and gender-specific reference intervals of healthy Chinese Han adults' lymphocyte subsets were established. There was no statistical difference in the results from the three flow cytometers. Gender affected the results of CD4 (%) and the absolute count of CD3CD16CD56, where CD4 (%) was higher in women, and the absolute count of CD3CD16CD56 was higher in men. Age mainly affected the CD4/CD8 ratio, which was statistically higher in groups aged over 40 years; the percentage and number of CD3CD19 were more elevated in age groups below 30 years; however, the difference was not statistically significant.

Conclusions: This study established the reference intervals of lymphocyte subsets for healthy Chinese Han adult populations under the standardized methods. This study was the first nationwide study in China to use a flow cytometry-based single-platform method to establish the reference intervals of lymphocyte subsets of the healthy Chinese Han adult population. Gender and age were shown to influence the results of lymphocyte subsets.
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http://dx.doi.org/10.21037/atm-21-4031DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8573445PMC
October 2021

In Silico Functional Prediction and Expression Analysis of C2H2 Zinc-Finger Family Transcription Factor Revealed Regulatory Role of in Maize Growth.

Front Genet 2021 5;12:770427. Epub 2021 Nov 5.

Faculty of Agriculture, Forestry and Food Engineering, Yibin University, Yibin, China.

The C2H2-zinc finger proteins (ZFP) comprise a large family of transcription factors with various functions in biological processes. In maize, the function regulation of C2H2- zine finger (ZF) genes are poorly understood. We conducted an evolution analysis and functional prediction of the maize C2H2-ZF gene family. Furthermore, the gene has been cloned and sequenced for further favorable allelic variation discovery. The phylogenetic analysis of the C2H2-ZF domain indicated that the position and sequence of the C2H2-ZF domain of the poly-zinc finger gene are relatively conserved during evolution, and the C2H2-ZF domain with the same position is highly conserved. The expression analysis of the C2H2-ZF gene family in 11 tissues at different growth stages of B73 inbred lines showed that genes with multiple transcripts were endowed with more functions. The expression analysis of the C2H2-ZF gene in P1 and P2 inbred lines under drought conditions showed that the C2H2-ZF genes were mainly subjected to negative regulation under drought stress. Functional prediction indicated that the maize C2H2-ZF gene is mainly involved in reproduction and development, especially concerning the formation of important agronomic traits in maize yield. Furthermore, sequencing and correlation analysis of the gene indicated that this gene was significantly associated with the SDW-NAP and TDW-NAP. The analysis of the relationship between maize C2H2-ZF genes and C2H2-ZF genes with known functions indicated that the functions of some C2H2-ZF genes are relatively conservative, and the functions of homologous genes in different species are similar.
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http://dx.doi.org/10.3389/fgene.2021.770427DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8602080PMC
November 2021

Study on the effect of CYP2C19 genetic polymorphism and plasma concentration on clopidogrel resistance.

Pak J Pharm Sci 2021 Sep;34(5):1685-1691

Department of Pharmacology, The First Affiliated Hospital of Kunming Medical University, Yunnan, China.

At present, the mechanism for clopidogrel resistance (CR) is incompletely understood. Here, we aimed to analyze of the association of plasma concentration of clopidogrel active metabolites (CAM) and CYP2C19 genetic polymorphism with CR. We assigned 77 patients to receive CLP at a loading dose of 300mg on day 1, followed by 75mg per day from day 2 to day 6. Three peripheral venous blood samples were collected for analysis. Our results showed that plasma concentration of CAM in extensive metabolizers (EMs) group (2.48(1.31, 5.67) ng/mL) was higher than that in intermediate metabolizers (IMs) group (1.44(1.18,3.55) ng/mL) and that in poor metabolizers (PMs) (1.18(1.12,1.33) ng/mL) group was the lowest (H=14.58, P=0.001). Besides, the incidence of CR in EMs group(11.1%) was lower than that in IMs group (20.0%) and that in IMs group was lower than that in PMs group (45.5%) (χ=6.344, P=0.042). In addition, our findings confirmed that the incidence of chest tightness in IMs group (40.0%) and PMs group (50.0%) was higher than that in EMs group (9.1%) (P=0.015). Over the follow-up period, it was found that CYP2C19 and plasma concentration of CAM were related to the incidence of chest tightness. Our findings indicated that in addition to CYP2C19, plasma concentration of CAM may be an important factor in predicting CR.
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September 2021

Multiparameter MRI Radiomics Model Predicts Preoperative Peritoneal Carcinomatosis in Ovarian Cancer.

Front Oncol 2021 21;11:765652. Epub 2021 Oct 21.

Department of Radiology, Inner Mongolia International Hospital, Hohhot, China.

Objectives: To evaluate the predictive value of radiomics features based on multiparameter magnetic resonance imaging (MP-MRI) for peritoneal carcinomatosis (PC) in patients with ovarian cancer (OC).

Methods: A total of 86 patients with epithelial OC were included in this retrospective study. All patients underwent FS-T2WI, DWI, and DCE-MRI scans, followed by total hysterectomy plus omentectomy. Quantitative imaging features were extracted from preoperative FS-T2WI, DWI, and DCE-MRI images, and feature screening was performed using a minimum redundancy maximum correlation (mRMR) and least absolute shrinkage selection operator (LASSO) methods. Four radiomics models were constructed based on three MRI sequences. Then, combined with radiomics characteristics and clinicopathological risk factors, a multi-factor Logistic regression method was used to construct a radiomics nomogram, and the performance of the radiomics nomogram was evaluated by receiver operating characteristic curve (ROC) curve, calibration curve, and decision curve analysis.

Results: The radiomics model from the MP-MRI combined sequence showed a higher area under the curve (AUC) than the model from FS-T2WI, DWI, and DCE-MRI alone (0.846 . 0.762, 0.830, 0.807, respectively). The radiomics nomogram (AUC=0.902) constructed by combining radiomics characteristics and clinicopathological risk factors showed a better diagnostic effect than the clinical model (AUC=0.858) and the radiomics model (AUC=0.846). The decision curve analysis shows that the radiomics nomogram has good clinical application value, and the calibration curve also proves that it has good stability.

Conclusion: Radiomics nomogram based on MP-MRI combined sequence showed good predictive accuracy for PC in patients with OC. This tool can be used to identify peritoneal carcinomatosis in OC patients before surgery.
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http://dx.doi.org/10.3389/fonc.2021.765652DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8591658PMC
October 2021

cFLIP Alleviates Myocardial Ischemia-Reperfusion Injury by Inhibiting Endoplasmic Reticulum Stress.

Cardiovasc Drugs Ther 2021 Nov 12. Epub 2021 Nov 12.

Institute of Cardiovascular Disease, China Three Gorges University, Yichang, 443003, China.

Purpose: Endoplasmic reticulum stress (ERS) plays a crucial role in myocardial ischemia-reperfusion injury (MIRI). Cellular FLICE-inhibitory protein (cFLIP) is an essential regulator of apoptosis and plays a major role in regulating ERS. The present study aimed to investigate the effects of long isoform cFLIP (cFLIP) on endogenous apoptosis and the mechanism of ERS in MIRI.

Methods: The cFLIP recombinant adenovirus vector was used to infect H9c2 cells and Sprague-Dawley (SD) rats. After infection for 72 h, ischemia was induced for 30 min, and reperfusion was then performed for 2 h to establish the MIRI model in SD rats. H9c2 cells were hypoxic for 4 h and then reoxygenated for 12 h to simulate ischemia/reperfusion (I/R) injury. Model parameters were evaluated by assessing cardiomyocyte viability, cell death (apoptosis), and ERS-related protein expression. In addition, tunicamycin (TM), an ERS agonist, was also added to the medium for pretreatment. Coimmunoprecipitation (Co-IP) of cFLIP and p38 MAPK protein was performed.

Results: cFLIP expression was decreased in I/R injury and hypoxia/reoxygenation (H/R) injury, and cFLIP overexpression reduced myocardial infarction in vivo and increased the viability of H9c2 cells in vitro. I/R and H/R upregulated the protein expression of GRP78, IRE-1, and PERK to induce ERS and apoptosis. Interestingly, overexpression of cFLIP significantly inhibited ERS and subsequent apoptosis, which was reversed by an agonist of ERS. Moreover, Co-IP showed that cFLIP attenuated ERS and was associated with inhibiting the activation of p38 protein.

Conclusion: The expression of cFLIP is significantly downregulated in MIRI, and it is accompanied by excessive ERS and apoptosis. Upregulated cFLIP suppresses ERS to reduce myocardial apoptosis, which is associated with inhibiting the activity of p38 MAPK. Therefore, cFLIP may be a potential intervention target for MIRI.
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http://dx.doi.org/10.1007/s10557-021-07280-1DOI Listing
November 2021

Spatial distribution and environmental impact factors of soil selenium in Hainan Island, China.

Sci Total Environ 2021 Oct 28:151329. Epub 2021 Oct 28.

Key Laboratory of Geochemical Exploration, Ministry of Nature Resources, Langfang 065000, China; Institute of Geophysical and Geochemical Exploration, Chinese Academy of Geological Sciences, Langfang 065000, China.

Selenium is an essential micronutrient for the human body, given its various health benefits. However, Se deficiency is widespread globally, and dietary adjustment is a feasible way to supplement people's Se daily intake. The Multi-purpose Regional Geochemical Survey (NMPRGS) conducted in Hainan Island found an abundance of Se-rich soil. These Se resources have been utilized to grow naturally Se-rich produce. However, insufficient research has been conducted into the spatial distribution and enrichment of soil Se in Hainan Island. This paper analysed the effect of the environmental impact factors (parent rock, precipitation, etc.) on soil Se, using data from the NMPRGS database. The results showed that, in comparison to the baseline value of Chinese soil, the enrichment degree of Se in the topsoil of Hainan Island was higher, but its distribution was uneven. The parent rock, precipitation, soil type, and soil characteristics all affect the concentration and spatial distribution of regional soil Se. Geographically weighted regression showed that Iodine, pH, SOC, and TFeO have a non-stationarity spatial relationship with Se. There was a significant correlation between soil Se and Chemical alteration index (CIA) in granite areas, while CIA was also related to mean annual precipitation (MAP). The concentration enrichment factor values of Se show that the external input of Se in high MAP areas is significantly higher than that in low MAP areas. Based on these results, three important environmental impact factors on soil Se enrichment at the regional scale in Hainan Island were defined: 1) Se-rich rocks; 2) precipitation; 3) SOC. These results can provide guidance for the planning and layout of Se-rich agriculture.
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http://dx.doi.org/10.1016/j.scitotenv.2021.151329DOI Listing
October 2021

Reprogramming the metabolism of PCC 6803 by regulating the plastoquinone biosynthesis.

Synth Syst Biotechnol 2021 Dec 23;6(4):351-359. Epub 2021 Oct 23.

State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, Shanghai, 200237, PR China.

Cyanobacteria can utilize CO or even N to produce a variety of high value-added products efficiently. Plastoquinone (PQ) is an important electron carrier in both of the photosynthetic and respiratory electron transport chain. Although the content of PQ, as well as their redox state, have an important effect on physiology and metabolism, there are relatively few studies on the synthesis of PQ and its related metabolic regulation mechanism in photosynthetic microorganisms. In this study, the strategies of overexpression of Geranyl diphosphate: 4-hydroxybenzoate geranyltransferase () and addition of 4-hydroxybenzoate (4-HB) as the quinone ring precursor were adopted to regulate the biosynthesis of PQ in PCC 6803. Combined with the analysis the photosystem activity, respiration rate and metabolic components, we found the changes of intracellular PQ reprogrammed the metabolism of PCC 6803. The results showed that the overexpression of reduced PQ content dramatically, by 22.18%. Interestingly, both of the photosynthesis and respiration rate were enhanced. In addition, the intracellular lipid and protein contents were significantly increased. Whereas, the addition of low concentrations of 4-HB enhanced the biosynthesis of PQ, and the intracellular PQ contents were increased by 14.76%-70.86% in different conditions. Addition of 4-HB can regulate the photosystem efficiency and respiration and reprogram the metabolism of PCC 6803 efficiently. In a word, regulating the PQ biosynthesis provided a novel idea for promoting the reprogramming the physiology and metabolism of .
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http://dx.doi.org/10.1016/j.synbio.2021.10.004DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8554343PMC
December 2021

The influence of workplace incivility on the fatigue of female nurses:the mediating effect of engagement.

J Nurs Manag 2021 Nov 8. Epub 2021 Nov 8.

School of Public Health, China Medical University, Shenyang, China.

Aims: To identify the associations of workplace incivility, engagement and fatigue among Chinese female nurses, and further explore whether engagement can play a mediating role.

Background: Nurses are at a high risk of fatigue. However, no research has been done to examine the associations among nurses' workplace incivility, engagement and fatigue.

Methods: The cross-sectional study was conducted in Jiangsu Province, China. Self-administered questionnaires were distributed to 1,200 female nurses, including The Fatigue Scale, Workplace Incivility Scale, Gallup Workplace Questionnaire and demographic variables. Valid responses were obtained from 1,035 (86.3%) of participants. Hierarchical multiple regression analysis was performed to examine the associations among workplace incivility, engagement and fatigue.

Results: The mean fatigue score was 6.54±3.07. Workplace incivility and engagement were related to fatigue (P<0.01). Engagement partly mediated the association between workplace incivility and fatigue (a*b =0.086, bias-corrected 95% CI: 0.059, 0.116; P<0.01), and the proportion of the mediating effect accounted for by engagement was 33.0%.

Conclusion: Chinese female nurses suffered from high level of fatigue. The improvement of female nurses' engagement may be helpful to alleviate the impact of workplace incivility on fatigue.

Implications For Nursing Management: Managers should reduce workplace incivility of female nurses by promoting engagement to reduce fatigue.
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http://dx.doi.org/10.1111/jonm.13507DOI Listing
November 2021

The ZO-1 protein Polychaetoid as an upstream regulator of the Hippo pathway in Drosophila.

PLoS Genet 2021 Nov 8;17(11):e1009894. Epub 2021 Nov 8.

Integrative Biomedical and Diagnostic Sciences Department, School of Dentistry, Oregon Health and Science University, Portland, Oregon, United States of America.

The generation of a diversity of photoreceptor (PR) subtypes with different spectral sensitivities is essential for color vision in animals. In the Drosophila eye, the Hippo pathway has been implicated in blue- and green-sensitive PR subtype fate specification. Specifically, Hippo pathway activation promotes green-sensitive PR fate at the expense of blue-sensitive PRs. Here, using a sensitized triple heterozygote-based genetic screening approach, we report the identification of the single Drosophila zonula occludens-1 (ZO-1) protein Polychaetoid (Pyd) as a new regulator of the Hippo pathway during the blue- and green-sensitive PR subtype binary fate choice. We demonstrate that Pyd acts upstream of the core components and the upstream regulator Pez in the Hippo pathway. Furthermore, We found that Pyd represses the activity of Su(dx), a E3 ligase that negatively regulates Pez and can physically interact with Pyd, during PR subtype fate specification. Together, our results identify a new mechanism underlying the Hippo signaling pathway in post-mitotic neuronal fate specification.
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http://dx.doi.org/10.1371/journal.pgen.1009894DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8610254PMC
November 2021

The Association Between Depressive Symptoms and Insomnia in College Students in Qinghai Province: The Mediating Effect of Rumination.

Front Psychiatry 2021 20;12:751411. Epub 2021 Oct 20.

Department of Psychiatry, Nanfang Hospital, Southern Medical University, Guangzhou, China.

This study investigates the mediating effect of rumination on the associations between depressive symptoms and insomnia. This is a cross-sectional study. Insomnia Severity Index (ISI), Ruminant Response Scale (RRS) and Beck Depression Inventory (BDI) were determined in 12,178 college students in Qinghai province by a questionnaire network platform. The prevalence of insomnia was 38.6% in the participants. Insomnia symptoms [interquartile range: 6 (3, 9)], depressive symptoms [interquartile range: 5 (1, 9)], and rumination [interquartile range: 22 (20, 26)] were positively correlated ( = 0.25-0.46, < 0.01). Mediation effect analysis showed that the depressive symptoms affected insomnia directly and indirectly. The direct effect and the indirect effect through rumination account for 92.4 and 7.6% of the total effect, respectively. The study shows that insomnia, depressive symptoms, and rumination are related constructs in college students in Qinghai province. It demonstrates the direct effects and the rumination-mediated indirect effects between depressive symptoms and insomnia; the direct effects seem to be dominant.
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http://dx.doi.org/10.3389/fpsyt.2021.751411DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8563788PMC
October 2021

Structure and diversity of fungal communities in long-term copper-contaminated agricultural soil.

Sci Total Environ 2022 Feb 28;806(Pt 3):151302. Epub 2021 Oct 28.

State Key Laboratory of Urban and Regional Ecology, Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences, Beijing 100085, China; University of Chinese Academy of Sciences, Beijing 100049, China.

Copper (Cu) contamination threatens the stability of soil ecosystems. As important moderators of biochemical processes and soil remediation, the fungal community in contaminated soils has attracted much research interest. In this study, soil fungal diversity and community composition under long-term Cu contamination were investigated based on high-throughput sequencing. The co-occurrence networks were also constructed to display the co-occurrence patterns of the soil fungal community. The results showed that the richness and Chao1 index both significantly increased at 50 mg kg Cu and then significantly decreased at 1600 and 3200 mg kg Cu. Soil fungal diversity was significantly and positively correlated with plant dry weight. Specific tolerant taxa under different Cu contamination gradients were illustrated by linear discriminant analysis effect size (LEfSe). Soil Cu concentration and shoot dry weight were the strongest driving factors influencing fungal composition. The relative abundance of arbuscular mycorrhizal fungi increased first and then declined along with elevating Cu concentrations via FUNGuild analysis. The interactions among fungi were enhanced under light and moderate Cu contamination but weakened under heavy Cu contamination by random matrix theory (RMT)-based molecular ecological network analysis. Penicillium, identified as a keystone taxon in Cu-contaminated soils, had the function of removing heavy metals and detoxification, which might be vital to trigger the resistance of the fungal community to Cu contamination. The results may facilitate the identification of Cu pollution indicators and the development of in situ bioremediation technology for contaminated cultivated fields.
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http://dx.doi.org/10.1016/j.scitotenv.2021.151302DOI Listing
February 2022

Polysaccharide Alleviates Intestinal Inflammation by Promoting Small Extracellular Vesicle Packaging of miR-433-3p.

J Agric Food Chem 2021 Nov 5;69(45):13510-13523. Epub 2021 Nov 5.

Guangdong Provincial Key Laboratory of Lingnan Specialty Food Science and Technology, Guangzhou City, Guangdong 510642, China.

polysaccharide (DOP) attenuates inflammatory bowel disease (IBD), but its role in regulating cross-talk between intestinal epithelial cells (IEC) and macrophages against IBD is unclear. This study aimed to investigate DOP protective effects on the intestinal inflammatory response through regulation by miRNA in small extracellular vesicles (sEVs). Our results show that DOP interfered with the secretion of small extracellular vesicles (DIEs) by IEC, which reduced the levels of inflammatory mediators. Increased miR-433-3p expression in DIEs was identified as an important protector against intestinal inflammation. DOP regulated the loading of miR-433-3p by hnRNPA2B1 into the intestinal sEV to increase the abundance of miR-433-3p. DIEs delivered miR-433-3p to lipopolysaccharide-induced macrophages and targeted the MAPK8 gene, leading to inhibition of the MAPK signaling pathway and reduced production of inflammatory cytokines. One protective mechanism of DOP is mediated by intestinal sEV containing miR-433-3p, which is a potential therapeutic agent for the prevention of inflammatory factor accumulation from excessive intestinal macrophage activity and for restoring homeostasis in the intestinal microenvironment.
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http://dx.doi.org/10.1021/acs.jafc.1c05134DOI Listing
November 2021

Differences in Diet and Gut Microbiota Between Lactating and Non-lactating Asian Particolored Bats (): Implication for a Connection Between Diet and Gut Microbiota.

Front Microbiol 2021 12;12:735122. Epub 2021 Oct 12.

College of Life Science, Jilin Agricultural University, Changchun, China.

In mammals, lactation is considered the most energetically costly phase for females. To meet nutritional and energy demands, lactating females usually change feeding patterns by eating food that is higher in protein and calories. Their gut microbes respond accordingly to help adapt to the changes in diet. In this study, we examined differences in diet and gut microbial composition between lactating and non-lactating Asian particolored bats () using COI and 16S amplicon sequencing. When compared with non-lactating bats, we found that the diversity and composition of lactating bats' diets differed; the proportion of Diptera increased and Coleoptera and Orthoptera decreased significantly. This could be attributed to the easy availability and high protein content of Diptera. Comparative analysis of the gut microbiota of lactating and non-lactating females showed that although the diversity of gut microbiota did not change, the relative abundance of specific gut microbiota associated with a particular diet did change. For example, when the consumption of Coleoptera decreased in lactating bats, the relative abundance of Lactobacillaceae was also reduced. Lactobacillaceae are thought to be involved in the digestion of Coleopteran exoskeletons. This study suggests that during lactation, Asian particolored bats eat a diet that yields higher levels of protein, and at the same time, the abundance of specific gut microbes change to help their hosts adapt to these changes in diet.
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http://dx.doi.org/10.3389/fmicb.2021.735122DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8546350PMC
October 2021

Design and Preparation of "corn-like" [email protected] Assembly for Improvement of Effective Internalization.

Int J Nanomedicine 2021 19;16:7091-7102. Epub 2021 Oct 19.

College of Food Science and Technology, Northwest University, Xi'an, Shaanxi, 710069, People's Republic of China.

Purpose: Superparamagnetic iron oxide nanoparticles (SPIONs) have exhibited preeminent diagnosis and treatment performances, but their low internalization severely limits predesigned functions. The low cell internalization is now an urgent bottleneck problem for almost all nanomaterials. To achieve more internalization of SPIONS, recombinant M13 phage was designed for targeted delivery and smart release.

Methods: M13 phages were designed to co-express exogenous SPARC binding peptide (SBP) and cathepsin B cleavage peptide (DFK), formed recombinant DFK-SBP-M13. 3.37± 0.06 nm of SPIONs were modified by 3, 4-dihydroxyhydrocinnamic acid (DHCA) to gain 10.80 ± 0.21 nm of DHCA-coated SPIONs, i.e., [email protected] Upon adjusting the proportions of [email protected] and DFK-SBP-M13, the multi-carboxyl SPIONs assembled onto recombinant M13 phages via covalent bonding. The assemblies were co-cultured with MDA-MB-231 cells to interpret their internalization and smart release.

Results: The "corn-like" [email protected] (261.47±3.30 nm) assemblies have not been reported previously. The assembly was stable, dispersible, superparamagnetic and biocompatible. After co-cultivation with MDA-MB-231 cells, the [email protected] assemblies quickly bond to the cell surface and are internalized. The enrichment rate of [email protected] assembly was 13.9 times higher than free SPIONs at 0.5 h, and intracellular Fe content was 3.6 times higher at 1 h. Furthermore, the DFK peptides favored cathepsin B to cleave SPIONs from the M13 templates resulting in release of SPIONs inside cells.

Conclusion: The novel [email protected] assembly can rapidly deliver SPIONs to the targeted sites and enabled smart release. The combination of genetic recombination and nanotechnology is beneficial for designing and optimizing some new nanomaterials with special functions to achieve wider applications.
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http://dx.doi.org/10.2147/IJN.S325282DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8541766PMC
November 2021

The Chinese Society of Clinical Oncology (CSCO) clinical guidelines for the diagnosis and treatment of nasopharyngeal carcinoma.

Cancer Commun (Lond) 2021 Nov 26;41(11):1195-1227. Epub 2021 Oct 26.

Department of Radiation Oncology, The First Affiliated Hospital of Guangxi Medical University, Nanning, Guangxi, 530000, P. R. China.

Nasopharyngeal carcinoma (NPC) is a malignant epithelial tumor originating in the nasopharynx and has a high incidence in Southeast Asia and North Africa. To develop these comprehensive guidelines for the diagnosis and management of NPC, the Chinese Society of Clinical Oncology (CSCO) arranged a multi-disciplinary team comprising of experts from all sub-specialties of NPC to write, discuss, and revise the guidelines. Based on the findings of evidence-based medicine in China and abroad, domestic experts have iteratively developed these guidelines to provide proper management of NPC. Overall, the guidelines describe the screening, clinical and pathological diagnosis, staging and risk assessment, therapies, and follow-up of NPC, which aim to improve the management of NPC.
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http://dx.doi.org/10.1002/cac2.12218DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8626602PMC
November 2021

Transcriptome and Metabolome Analysis Revealed the Freezing Resistance Mechanism in 60-Year-Old Overwintering .

Biology (Basel) 2021 Oct 3;10(10). Epub 2021 Oct 3.

Agricultural Big-Data Research Center and College of Plant Protection, Shandong Agricultural University, Taian 271018, China.

Freezing stress in winter is the biggest obstacle to the survival of in mid-latitude and high-latitude areas, which has a great impact on the yield, quality, and even life of every year. In this study, transcriptome and metabolome were used to clarify the freezing resistance mechanism of 60-year-old natural overwintering under freezing stress. Next, 3880 DEGs and 353 DAMs were obtained. The enrichment analysis showed that pathways of MAPK and ABA played a key role in the signal transduction of freezing stress, and in the ABA pathway promoted stomatal closure. Then, the water holding capacity and the freezing resistance of were improved. The pathway analysis showed that DEGs and DAMs were significantly enriched and up-regulated in the three-related pathways of phenylpropanoid biosynthesis, flavone and flavonol biosynthesis, and flavonoid biosynthesis. In addition, the carbohydrate and fatty acid synthesis pathways also had a significant enrichment, and the synthesis of these substances facilitated the freezing resistance. These results are of great significance to elucidate the freezing resistance mechanism and the freezing resistance breeding of .
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http://dx.doi.org/10.3390/biology10100996DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8533452PMC
October 2021

Data Quality Improvement and Internal Data Audit of the Chinese Neonatal Network Data Collection System.

Front Pediatr 2021 4;9:711200. Epub 2021 Oct 4.

Division of Neonatology, The Children's Hospital of Zhejiang University School of Medicine, Hangzhou, China.

The Chinese Neonatal Network (CHNN) is a nationwide neonatal network that aims to improve clinical neonatal care quality and short- and long-term health outcomes of infants. This study aims to assess the quality of the Chinese Neonatal Network database by conducting an internal audit of data extraction. A data audit was performed by independently replicating the data collection and entry process in all 58 tertiary neonatal intensive care units (NICU) participating in the CHNN. Eighty-eight data elements selected for re-abstraction were classified into three categories (critical, important, less important), and agreement rates for original and re-abstracted data were predefined. Three to five records were randomly selected at each site for re-abstraction, including one short- (0-7 days), two medium- (8-28 days), and two long-stay (more than 28 days) cases. Agreement rates for each data item were calculated for individual NICUs and across the network, respectively. A total of 283 cases and 24,904 data fields were re-abstracted. The agreement rates for original and re-abstracted data elements were 96.1% overall, and 97.2, 94.3, and 96.6% for critical, important, and less important data elements, respectively. Individual site variation for discrepancies ranged between 0.0 and 18.4% for all collected data elements. The completeness, precision, and quality of data in the CHNN database are high, providing assurance for multipurpose use, including health service evaluation, quality improvement, clinical trials, and other research.
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http://dx.doi.org/10.3389/fped.2021.711200DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8522580PMC
October 2021

Identification of a novel IRF8 homozygous mutation causing neutrophilia, monocytopenia and fatal infection in a female neonate.

Infect Genet Evol 2021 Oct 16;96:105121. Epub 2021 Oct 16.

Department of Neonatology, First Hospital of Jilin University, Changchun 130021, China. Electronic address:

Inborn errors of immunity (IEIs) result from mutations in genes involved in host immune defense and immune regulation. Herein, we report the identification of a novel IRF8 mutation in a neonate with an IEI. DNA samples from both the neonate and her parents were subjected to DNA sequencing, and the immune status of the patient was assessed. We identified a mutation (c.331C > T, p. Arg111*) in the interferon regulatory factor 8 (IRF8) gene that manifested as sever dysfunctional neutrophilia (96.53 × 10/l) and monocytopenia (0.02 × 10/l). The patient's CD3 T cell and CD8 T cell counts were decreased. Her levels of IFN-γ were low even during severe infection. The mRNA expression levels of IRF8 were lower than normal. Her clinical manifestations included a recurrent and progressively fatal infection. Since IRF8 plays a key role in the differentiation and development of immune cells, we suspected that the novel mutation (c.331C > T, p. Arg111*) may be consistent with a severe loss of IRF8 function and result in a failure of immune cells to differentiate and maturation, and lead to a severe infection with early onset.
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http://dx.doi.org/10.1016/j.meegid.2021.105121DOI Listing
October 2021

Rotary Valve-Assisted Fluidic System Coupling with CRISPR/Cas12a for Fully Integrated Nucleic Acid Detection.

ACS Sens 2021 Nov 19;6(11):4048-4056. Epub 2021 Oct 19.

College of Biosystems Engineering and Food Science, Zhejiang University, Hangzhou 310058, China.

Of late, many nucleic acid analysis platforms have been established, but there is still room for constructing integrated nucleic acid detection systems with high nucleic acid extraction efficiency, low detection cost, and convenient operation. In this work, a simple rotary valve-assisted fluidic chip coupling with CRISPR/Cas12a was established to achieve fully integrated nucleic acid detection. All of the detection reagents were prestored on the fluidic chip. With the aid of the rotary valve and syringe, the liquid flow and stirring can be precisely controlled. The nucleic acid extraction, loop-mediated isothermal amplification (LAMP) reaction, and CRISPR detection could be completed in 80 min. A clean reservoir and an air reservoir on the fluidic chip were designed to effectively remove the remaining ethanol. With as the targets, the detection sensitivity of the fluidic chip could reach 3.1 × 10 copies of target DNA per reaction. A positive sample could be sensitively detected by CRISPR/Cas12a to produce a green fluorescent signal, while a negative sample generated no fluorescent signal. Further, the fluidic chip was successfully applied for detection of spiked shrimp samples, which showed the same detection sensitivity. A great feasibility for real-sample detection was showed by the fluidic chip. The proposed detection platform did not need expensive centrifugal instruments or pumps, which displayed its potential to become a powerful tool for food safety analysis and clinical diagnostics, especially in the resource-limited areas.
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http://dx.doi.org/10.1021/acssensors.1c01468DOI Listing
November 2021

The Intraoperative Median Effective Dose of Oxytocin for Preventing Uterine Atony in Parturients with a Prior History of Caesarean Delivery.

Clin Drug Investig 2021 Dec 16;41(12):1047-1053. Epub 2021 Oct 16.

Department of Anesthesia, Women's Hospital, Zhejiang University School of Medicine, Xueshi Road 1, Hangzhou, 310006, China.

Background And Objective: While oxytocin is commonly used for the prevention of uterine atony, its pharmacology may be affected by a prior history of caesarean delivery. The objective of this study was to determine the 50% effective dose (ED) of bolus oxytocin after caesarean delivery in parturients with and without prior caesarean delivery.

Methods: This was a parallel-group, double-blind, dose-response study using Dixon's up-and-down sequential allocation method to estimate the ED of bolus-administered oxytocin in parturients having caesarean delivery under combined spinal-epidural anaesthesia (CSE). Twenty-seven parturients with a history of prior caesarean delivery (With-PCD group) and 26 parturients with no such history (Without-PCD group) were enrolled. Oxytocin was administered as an intravenous bolus at a starting dose of 0.5 units, which was then increased or decreased by 0.25 units at a time. Uterine tone was assessed by the obstetrician as either 'adequate' or 'inadequate' 3 min after delivery of the fetus. Adverse effects, administration of additional uterotonic agents, and estimated blood loss were recorded.

Results: The ED of oxytocin was greater in the With-PCD group than in the Without-PCD group (0.95 units [95% CI 0.82-1.08] vs. 0.55 units [95% CI 0.38-0.73], P < 0.001). The overall incidence of adverse effects was higher in the With-PCD group than in the Without-PCD group (33.3% vs. 7.7%, P = 0.02).

Conclusion: The initial bolus dose of oxytocin needed to prevent uterine atony was higher in parturients with prior caesarean delivery than in parturients without prior caesarean delivery. Uterine scarring may contribute to the increased oxytocin requirements of the former group.

Trial Registration Number: ChiCTR1900023474; investigator: Wei CN; date of registration: 30 May 2019.
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http://dx.doi.org/10.1007/s40261-021-01090-xDOI Listing
December 2021

Psychosocial and psychological interventions for relapse prevention in schizophrenia: a systematic review and network meta-analysis.

Lancet Psychiatry 2021 11 12;8(11):969-980. Epub 2021 Oct 12.

Department of Psychiatry and Psychotherapy, School of Medicine, Technical University of Munich, Munich, Germany. Electronic address:

Background: Many psychosocial and psychological interventions are used in patients with schizophrenia, but their comparative efficacy in the prevention of relapse is not known. We aimed to evaluate the efficacy, acceptability, and tolerability of psychosocial and psychological interventions for relapse prevention in schizophrenia.

Methods: To conduct this systematic review and network meta-analysis we searched for published and unpublished randomised controlled trials that investigated psychosocial or psychological interventions aimed at preventing relapse in patients with schizophrenia. We searched EMBASE, MEDLINE, PsycINFO, BIOSIS, Cochrane Library, WHO International Clinical Trials Registry Platform, and ClinicalTrials.gov up to Jan 20, 2020, and searched PubMed up to April 14, 2020. We included open and masked studies done in adults with schizophrenia or related disorders. We excluded studies in which all patients were acutely ill, had a concomitant medical or psychiatric disorder, or were prodromal or "at risk of psychosis". Study selection and data extraction were done by two reviewers independently based on published and unpublished reports, and by contacting study authors. Data were extracted about efficacy, tolerability, and acceptability of the interventions; potential effect moderators; and study quality and characteristics. The primary outcome was relapse measured with operationalised criteria or psychiatric hospital admissions. We did random-effects network meta-analysis to calculate odds ratios (ORs) or standardised mean differences (SMDs) with 95% CIs. The study protocol was registered with PROSPERO, CRD42019147884.

Findings: We identified 27 765 studies through the database search and 330 through references of previous reviews and studies. We screened 28 000 records after duplicates were removed. 24 406 records were excluded by title and abstract screening and 3594 full-text articles were assessed for eligibility. 3350 articles were then excluded for a variety of reasons, and 244 full-text articles corresponding to 85 studies were included in the qualitative synthesis. Of these, 72 studies with 10 364 participants (3939 females and 5716 males with sex indicated) were included in the network meta-analysis. The randomised controlled trials included compared 20 psychological interventions given mainly as add-on to antipsychotics. Ethnicity data were not available. Family interventions (OR 0·35, 95% CI 0·24-0·52), relapse prevention programmes (OR 0·33, 0·14-0·79), cognitive behavioural therapy (OR 0·45, 0·27-0·75), family psychoeducation (OR 0·56, 0·39-0·82), integrated interventions (OR 0·62, 0·44-0·87), and patient psychoeducation (OR 0·63, 0·42-0·94) reduced relapse more than treatment as usual at 1 year. The confidence in the estimates ranged from moderate to very low. We found no indication of publication bias.

Interpretation: We found robust benefits in reducing the risk of relapse for family interventions, family psychoeducation, and cognitive behavioral therapy. These treatments should be the first psychosocial interventions to be considered in the long-term treatment for patients with schizophrenia.

Funding: German Ministry for Education and Research.
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http://dx.doi.org/10.1016/S2215-0366(21)00243-1DOI Listing
November 2021

Effects of enzymatic hydrolysis on physicochemical property and antioxidant activity of mulberry ( Roxb.) leaf protein.

Food Sci Nutr 2021 Oct 25;9(10):5379-5390. Epub 2021 Aug 25.

Department of Food Biotechnology Research Institute of Food Science and Technology (RIFST) Mashhad Iran.

To improve the antioxidant efficiency of mulberry leaf protein (MLP), alcalase, protamex, papain, flavourzyme, neutrase, and trypsin were used to hydrolyze MLP. The yield of soluble peptides, secondary structures, molecular weight distributions, and antioxidant activities of MLP hydrolysates (MLPHs) were investigated. Results showed that the native MLP was rich in the fraction above 6.5 kDa and was mainly composed of β-sheets, while MLPHs were abundant in the fractions of 0.3-0.6 kDa and 0.6-6.5 kDa and were mainly composed of disordered coils and β-folds. Limited hydrolysis of MLP could lead to better antioxidant activity than extensive hydrolysis. After enzymatic hydrolysis, the content of total sugar and total phenol in MLP increased significantly. MLP hydrolysates prepared with neutrase, alcalase, and protamex were preferable to other enzymes. Meanwhile, an enzyme to substrate level of 1% and a hydrolysis time of 2 hr were the optimum conditions to obtain higher antioxidant hydrolysates using neutrase.
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http://dx.doi.org/10.1002/fsn3.2474DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8498073PMC
October 2021

Evaluation and validation of a novel 10-color flow cytometer.

J Clin Lab Anal 2021 Nov 12;35(11):e23834. Epub 2021 Oct 12.

Department of Laboratory Medicine, Zhongshan Hospital, Fudan University, Shanghai, China.

Background: Flow cytometry is a powerful technique that provides information regarding cell properties. In this study, we evaluated the analytical performance of a new flow cytometer, the 10-color BD FACSLyric , which could help doctors obtain reliable test results prior to clinical research.

Methods: We used Sphero Rainbow Calibration Particles and the Sphero Nano Fluorescent Particle Size Standard Kit to validate the fluorescence sensitivity and linearity. The Beckman Coulter IMMUNO-TROL Cell was used as the quality control to evaluate the accuracy and reproducibility of surface markers detected by the flow cytometer. Furthermore, BD Calibrate APC Beads and CS&T Research Beads were applied to calculate the carry-over contamination rate and assess the instrument stability.

Results: A linear regression equation between the molecules of equivalent soluble fluorochrome and fluorescence detection limit showed a good linear fit (R  > 0.99). The minimum bead size detected by side scatter was 0.22 μm. The coefficient of variation percentage of each fluorescence channel was below 2%, and the carry-over contamination rate of the cytometer was under 0.2%. After running the BD FACSLyric cytometer continuously for 8 h, the median fluorescence index of particles remained close to that at the time of cytometer startup.

Conclusions: The 10-color BD FACSLyric cytometer showed good performance in the evaluation performed in this study and may be trusted to provide accurate results for clinical research.
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http://dx.doi.org/10.1002/jcla.23834DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8605138PMC
November 2021

Cardioprotective Effect of Stem-Leaf Saponins From on Mice With Sleep Derivation by Inhibiting Abnormal Autophagy Through PI3K/Akt/mTOR Pathway.

Front Cardiovasc Med 2021 16;8:694219. Epub 2021 Sep 16.

Shanghai Key Laboratory of Compound Chinese Medicines, The Ministry of Education (MOE) Key Laboratory for Standardization of Chinese Medicines, The State Administration of TCM (SATCM) Key Laboratory for New Resources and Quality Evaluation of Chinese Medicine, Institute of Chinese Materia Medica, Shanghai University of Traditional Chinese Medicine, Shanghai, China.

Sleep deprivation (SD) may lead to serious myocardial injury in cardiovascular diseases. Saponins extracted from the roots of , a traditional Chinese medicine beneficial to blood circulation and hemostasis, are the main bioactive components exerting cardiovascular protection in the treatment of heart disorders, such as arrhythmia, ischemia and reperfusion injury, and cardiac hypertrophy. This study aimed to explore the protective effect of stem-leaf saponins from (SLSP) on myocardial injury in SD mice. SD was induced by a modified multi-platform method. Cardiac morphological changes were assessed by hematoxylin and eosin (H&E) staining. Heart rate and ejection fraction were detected by specific instruments. Serum levels of atrial natriuretic peptide (ANP) and lactate dehydrogenase (LDH) were measured with biochemical kits. Transmission electron microscopy (TEM), immunofluorescent, and Western blotting analysis were used to observe the process and pathway of autophagy and apoptosis in heart tissue of SD mice. , rat H9c2 cells pretreated with rapamycin and the effect of SLSP were explored by acridine orange staining, transient transfection, flow cytometry, and Western blotting analysis. SLSP prevented myocardial injury, such as morphological damage, accumulation of autophagosomes in heart tissue, abnormal high heart rate, serum ANP, and serum LDH induced by SD. In addition, it reversed the expressions of proteins involved in the autophagy and apoptosis and activated PI3K/Akt/mTOR signaling pathway that is disturbed by SD. On H9c2 cells induced by rapamycin, SLSP could markedly resume the abnormal autophagy and apoptosis. Collectively, SLSP attenuated excessive autophagy and apoptosis in myocardial cells in heart tissue induced by SD, which might be acted through activating PI3K/Akt/mTOR signaling pathway.
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http://dx.doi.org/10.3389/fcvm.2021.694219DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8483245PMC
September 2021

Predictors of prolonged grief disorder in Chinese elderly shidu parents: The role of perceived stigma and perceived stress.

Asia Pac Psychiatry 2021 Sep 30:e12489. Epub 2021 Sep 30.

Department of Social Medicine, College of Health Management, China Medical University, Shenyang, China.

Introduction: Parents who have lost their only child and over the age of 60 are known as elderly shidu parents. This study aimed to investigate predictors of prolonged grief disorder (PGD) among Chinese elderly shidu parents.

Methods: Two hundred and ninety-eight elderly shidu parents from Shenyang were included in this cross-sectional study. Participants completed a questionnaire including the Prolonged Grief questionnaire-13 (PG-13), the PTSD (post-traumatic stress disorder) Checklist for DSM-5 (PCL-5), the Center for Epidemiologic Studies Depression Scales (CES-D), the Self-Rating Anxiety Scale (SAS), and the Perceived Stress Scale-10 (PSS-10). Multiple linear regression was used to examine socio-demographic, bereavement-related and perceived stress associated with PGD symptoms.

Results: The prevalence of PGD among Chinese elderly shidu parents was 13.8%. Among the 41 PGD patients, 29, 39, 36 patients had comorbidity of PTSD, depressive symptoms and anxiety, respectively. Perceived stress, higher educational level, lower annual household income, perceived stigma, violent death of the child, and being in debt were associated with severer PGD symptoms.

Discussion: The present study revealed the prevalence of PGD and the important role of PGD in diagnosing other mental disorders in Chinese elderly shidu parents. Providing financial help, eliminating social stigma, and stress should be included in PGD prevention among Chinese elderly shidu parents.
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http://dx.doi.org/10.1111/appy.12489DOI Listing
September 2021

Molecular mechanisms of inhibiting glucosyltransferases for biofilm formation in Streptococcus mutans.

Int J Oral Sci 2021 09 30;13(1):30. Epub 2021 Sep 30.

State Key Laboratory of Oral Diseases, National Clinical Research Center for Oral Diseases and Department of Pediatric Dentistry, West China Hospital of Stomatology, Sichuan University, Chengdu, China.

Glucosyltransferases (Gtfs) play critical roles in the etiology and pathogenesis of Streptococcus mutans (S. mutans)- mediated dental caries including early childhood caries. Gtfs enhance the biofilm formation and promotes colonization of cariogenic bacteria by generating biofilm extracellular polysaccharides (EPSs), the key virulence property in the cariogenic process. Therefore, Gtfs have become an appealing target for effective therapeutic interventions that inhibit cariogenic biofilms. Importantly, targeting Gtfs selectively impairs the S. mutans virulence without affecting S. mutans existence or the existence of other species in the oral cavity. Over the past decade, numerous Gtfs inhibitory molecules have been identified, mainly including natural and synthetic compounds and their derivatives, antibodies, and metal ions. These therapeutic agents exert their inhibitory role in inhibiting the expression gtf genes and the activities and secretion of Gtfs enzymes with a wide range of sensitivity and effectiveness. Understanding molecular mechanisms of inhibiting Gtfs will contribute to instructing drug combination strategies, which is more effective for inhibiting Gtfs than one drug or class of drugs. This review highlights our current understanding of Gtfs activities and their potential utility, and discusses challenges and opportunities for future exploration of Gtfs as a therapeutic target.
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http://dx.doi.org/10.1038/s41368-021-00137-1DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8481554PMC
September 2021
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