Publications by authors named "Hui Wei"

520 Publications

Evaluation of pretreatment telomere length as a prognostic marker in intermediate-risk acute myeloid leukemia.

Int J Lab Hematol 2021 Jul 21. Epub 2021 Jul 21.

State Key Laboratory of Experimental Hematology, National Clinical Research Center for Blood Diseases, Institute of Hematology & Blood Diseases Hospital, Chinese Academy of Medical Sciences & Peking Union Medical College, Tianjin, China.

Introduction: The current framework for risk stratification is still insufficient for highly heterogeneous intermediate-risk acute myeloid leukemia (IRC-AML), which lacks specific genomic abnormalities.

Methods: In order to incorporate novel biomarkers to refine current risk stratification strategies for patients with this subtype, we investigated pretreatment telomere length (TL), which is essential for maintaining genomic stability, in 204 adults with de novo AML (non-acute promyelocytic leukemia).

Results: We found that TL measured at diagnosis did not decrease with advancing age in 204 patients with AML (R  = 0.001, P = .695). A multivariate analysis demonstrated that short TL was independently associated with an inferior relapse-free survival (hazard ratio [HR] 3.08, 95% confidence interval [CI] 1.48-6.41, P = .003); event-free survival (HR 2.14, 95% CI 1.12-4.08, P = .021); and overall survival (HR 2.26, 95% CI 1.09-4.67, P = .028) in IRC-AML patients. In addition, IRC-AML patients with short TL also exhibited an increased cumulative incidence of hematologic relapse (HR 2.32, 95% CI 1.08-5.26, P = .032).

Conclusion: Short TL is an independent prognostic factor for poor prognosis in patients with IRC-AML and may represent a novel mechanism that links genomic stability and disease progression.
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http://dx.doi.org/10.1111/ijlh.13665DOI Listing
July 2021

Plant Secondary Metabolites with an Overview of .

Int J Mol Sci 2021 Jun 26;22(13). Epub 2021 Jun 26.

Co-Innovation Center for Sustainable Forestry in Southern China, Key Laboratory of Forest Genetics & Biotechnology, Ministry of Education, College of Biology and the Environment, Nanjing Forestry University, Nanjing 210037, China.

trees meet continuous difficulties from the environment through their life cycle. To warrant their durability and generation, trees exhibit various types of defenses, including the production of secondary metabolites. Syntheses derived from the shikimate-phenylpropanoid pathway are a varied and plentiful class of secondary metabolites manufactured in . Amongst other main classes of secondary metabolites in are fatty acid and terpenoid-derivatives. Many of the secondary metabolites made by trees have been functionally described. Any others have been associated with particular ecological or biological processes, such as resistance against pests and microbial pathogens or acclimatization to abiotic stresses. Still, the functions of many secondary metabolites are incompletely understood. Furthermore, many secondary metabolites have therapeutic effects, leading to more studies of secondary metabolites and their biosynthesis. This paper reviews the biosynthetic pathways and therapeutic impacts of secondary metabolites in using a genomics approach. Compared with bacteria, fewer known pathways produce secondary metabolites in despite having had its genome sequenced.
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http://dx.doi.org/10.3390/ijms22136890DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8268465PMC
June 2021

Effects of Vegetation Restoration on Soil Erosion on the Loess Plateau: A Case Study in the Ansai Watershed.

Int J Environ Res Public Health 2021 06 10;18(12). Epub 2021 Jun 10.

State Key Laboratory of Earth Surface Processes and Resource Ecology, Faculty of Geographical Science, Beijing Normal University, Beijing 100875, China.

Large-scale vegetation restoration greatly changed the soil erosion environment in the Loess Plateau since the implementation of the "Grain for Green Project" (GGP) in 1999. Evaluating the effects of vegetation restoration on soil erosion is significant to local soil and water conservation and vegetation construction. Taking the Ansai Watershed as the case area, this study calculated the soil erosion modulus from 2000 to 2015 under the initial and current scenarios of vegetation restoration, using the Chinese Soil Loess Equation (CSLE), based on rainfall and soil data, remote sensing images and socio-economic data. The effect of vegetation restoration on soil erosion was evaluated by comparing the average annual soil erosion modulus under two scenarios among 16 years. The results showed: (1) vegetation restoration significantly changed the local land use, characterized by the conversion of farmland to grassland, arboreal land, and shrub land. From 2000 to 2015, the area of arboreal land, shrub land, and grassland increased from 19.46 km, 19.43 km, and 719.49 km to 99.26 km, 75.97 km, and 1084.24 km; while the farmland area decreased from 547.90 km to 34.35 km; (2) the average annual soil erosion modulus from 2000 to 2015 under the initial and current scenarios of vegetation restoration was 114.44 t/(hm²·a) and 78.42 t/(hm²·a), respectively, with an average annual reduction of 4.81 × 10 t of soil erosion amount thanks to the vegetation restoration; (3) the dominant soil erosion intensity changed from "severe and light erosion" to "moderate and light erosion", vegetation restoration greatly improved the soil erosion environment in the study area; (4) areas with increased erosion and decreased erosion were alternately distributed, accounting for 48% and 52% of the total land area, and mainly distributed in the northwest and southeast of the watershed, respectively. Irrational land use changes in local areas (such as the conversion of farmland and grassland into construction land, etc.) and the ineffective implementation of vegetation restoration are the main reasons leading to the existence of areas with increased erosion.
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http://dx.doi.org/10.3390/ijerph18126266DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8296048PMC
June 2021

High-contrast OPCPA front end in high-power petawatt laser facility based on the ps-OPCPA seed system.

Opt Express 2021 May;29(11):15980-15994

A high-energy, high-beam-quality, high-contrast picosecond optical parametric chirped-pulse amplification (ps-OPCPA) laser system was demonstrated. The pulse from a femtosecond oscillator was stretched to 4 ps, after which it was amplified from 140 pJ to 600 µJ by an 8 ps/6 mJ pump laser in two non-collinear OPCPA stages. The total gain was >10, and the root mean square of the energy stability of the laser system was 1.6% in 10 h. The contrasts of the solid and fiber mode-locked femtosecond oscillator-seeded ps-OPCPA systems were compared, and a signal-to-noise ratio of >10 was achieved. Using this system, the contrast of the front end in high-power picosecond petawatt laser facility was improved by ∼40 dB to >10, beyond ∼200 ps ahead of the main pulse with an output level of 60 mJ.
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http://dx.doi.org/10.1364/OE.425420DOI Listing
May 2021

Gene Deletions and Prognostic Values in B-Linage Acute Lymphoblastic Leukemia.

Front Oncol 2021 2;11:677034. Epub 2021 Jun 2.

State Key Laboratory of Experimental Hematology, National Clinical Research Center for Blood Disease, Institute of Hematology and Blood Diseases Hospital, Chinese Academy of Medical Sciences, Tianjin, China.

Although pediatric-like treatment regimen has remarkably improved the survival rates of adults with acute lymphoblastic leukemia (ALL), the outcome of some adult patients is still poor owing to adverse genetic features. These molecular abnormalities, especially gene deletions, may be considered for the prognosis assessment for adult patients with ALL. In this study, using multiplex ligation-dependent probe amplification (MLPA) method, gene deletions were analyzed in from 211 adult B-ALL patients treated in our center. The data showed that 68.2% (144/211) adult B-ALL patients carried gene deletions, and the frequency is much higher in PhB-ALL patients. gene deletion is the most common gene deletion in adult B-ALL, followed by deletion. In PhB-ALL patients, the overall survival of patients with gene deletions is inferior to that of patients without any gene deletions. More obviously, patients with or deletion had a worse prognosis, whereas, allogeneic hematopoietic stem cell transplantation could improve OS in patients with deletion, but not in patients with CDKN2A/B deletion. Moreover, the outcome of PhB-ALL patients with double deletion of and may be much worse than that of patients with or alone. Minimal residual disease (MRD) was also analyzed together with gene deletions and demonstrated that gene deletions have a negative impact on survival only in MRD positive PhB-ALL patients. In conclusion, gene deletions are closely related with the prognosis of adult PhB-ALL patients.
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http://dx.doi.org/10.3389/fonc.2021.677034DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8206559PMC
June 2021

MicroRNA profiles in five pairs of early gastric cancer tissues and adjacent non-cancerous tissues.

Oncol Lett 2021 Aug 7;22(2):595. Epub 2021 Jun 7.

Department of Gastroenterology, The First Hospital of Lanzhou University, Lanzhou, Gansu 730000, P.R. China.

Approximately half of the world's gastric cancer cases and deaths occur in China. In addition, the incidence and mortality rates of gastric cancer in Gansu province in China are much higher than the average nationwide levels. The present study investigated microRNA (miRNA/miR) profiles in early gastric cancer (EGC) without specific symptoms. miRNA expression levels in five pairs of EGC tissues and adjacent non-cancerous mucosa tissues of patients from Gansu province in China were analyzed using a miRNA microarray. A total of 47 differentially expressed miRNAs (DEMs) were identified. Subsequently, mRNA expression profiles of three pairs of cancer tissues and adjacent non-cancerous tissues from 3 Asian patients with stage I or stage II gastric cancer (stage I/II; American Joint Committee on Cancer classification, Eighth Edition) were obtained from The Cancer Genome Atlas database, and differentially expressed genes (DEGs) were identified. The target genes of DEMs were filtered from the DEGs using the miRDB database and a miRNA-gene network was constructed. The functions of DEMs were evaluated using the tool for annotations of human miRNAs database, and via Gene Ontology analysis, Kyoto Encyclopedia of Genes and Genomes analysis and Gene Set Enrichment Analysis of the target genes. Finally, survival analyses of DEMs, which were in the miRNA-gene network, was performed. The results suggested that a number of miRNAs, including hsa-let-7a-5p, hsa-miR-27a-3p, hsa-miR-126-5p and hsa-miR-424-5p, may serve critical roles in EGC. The present study could provide a basis for the identification of EGC screening biomarkers. Furthermore, the present study may provide a basis for the exploration of the cause of the high incidence of gastric cancer in Gansu province from the perspective of miRNAs.
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http://dx.doi.org/10.3892/ol.2021.12856DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8200934PMC
August 2021

A global-scale screening of non-native aquatic organisms to identify potentially invasive species under current and future climate conditions.

Sci Total Environ 2021 Sep 20;788:147868. Epub 2021 May 20.

Animal and Plant Sciences Group, Edinburgh Napier University, Sighthill, Edinburgh EH11 4BN, UK.

The threat posed by invasive non-native species worldwide requires a global approach to identify which introduced species are likely to pose an elevated risk of impact to native species and ecosystems. To inform policy, stakeholders and management decisions on global threats to aquatic ecosystems, 195 assessors representing 120 risk assessment areas across all six inhabited continents screened 819 non-native species from 15 groups of aquatic organisms (freshwater, brackish, marine plants and animals) using the Aquatic Species Invasiveness Screening Kit. This multi-lingual decision-support tool for the risk screening of aquatic organisms provides assessors with risk scores for a species under current and future climate change conditions that, following a statistically based calibration, permits the accurate classification of species into high-, medium- and low-risk categories under current and predicted climate conditions. The 1730 screenings undertaken encompassed wide geographical areas (regions, political entities, parts thereof, water bodies, river basins, lake drainage basins, and marine regions), which permitted thresholds to be identified for almost all aquatic organismal groups screened as well as for tropical, temperate and continental climate classes, and for tropical and temperate marine ecoregions. In total, 33 species were identified as posing a 'very high risk' of being or becoming invasive, and the scores of several of these species under current climate increased under future climate conditions, primarily due to their wide thermal tolerances. The risk thresholds determined for taxonomic groups and climate zones provide a basis against which area-specific or climate-based calibrated thresholds may be interpreted. In turn, the risk rankings help decision-makers identify which species require an immediate 'rapid' management action (e.g. eradication, control) to avoid or mitigate adverse impacts, which require a full risk assessment, and which are to be restricted or banned with regard to importation and/or sale as ornamental or aquarium/fishery enhancement.
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http://dx.doi.org/10.1016/j.scitotenv.2021.147868DOI Listing
September 2021

A Higher Dose of Dasatinib May Increase the Possibility of Crossing the Blood-Brain Barrier in the Treatment of Patients With Philadelphia Chromosome-Positive Acute Lymphoblastic Leukemia.

Clin Ther 2021 Jun 10. Epub 2021 Jun 10.

State Key Laboratory of Experimental Hematology, National Clinical Research Center for Blood Diseases, Institute of Hematology & Blood Diseases Hospital, Chinese Academy of Medical Sciences & Peking Union Medical College, Tianjin, China. Electronic address:

Purpose: Dasatinib is a second-generation tyrosine kinase inhibitor with higher central nervous system (CNS) penetration compared with imatinib and nilotinib in in vitro studies. However, limited clinical data are available regarding the dosage and CNS penetration of dasatinib. The purpose of this study was to investigate the actual ability of dasatinib to cross the blood-brain barrier in patients with Philadelphia chromosome-positive acute lymphoblastic leukemia (Ph ALL).

Methods: Plasma and cerebrospinal fluid (CSF) samples collected from Ph ALL patients treated with dasatinib were analyzed by using an LC-MS/MS assay.

Findings: Orally administered dasatinib 100 mg once daily was well absorbed by the patient but penetrated poorly into the CSF. The use of a higher drug dosage (140 mg/d) may increase systemic drug exposure and enhance the penetration of dasatinib into the CSF.

Implications: Based on this study, the use of a higher dosage of dasatinib (140 mg/d) is recommended in patients at high risk of CNS relapse or patients who need treatment for CNS leukemia. ClinicalTrials.gov identifier: NCT02523976.
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http://dx.doi.org/10.1016/j.clinthera.2021.05.009DOI Listing
June 2021

Design of nanozymes for inflammatory bowel disease therapy.

Sci China Life Sci 2021 Jun 8. Epub 2021 Jun 8.

Department of Biomedical Engineering, College of Engineering and Applied Sciences, Nanjing National Laboratory of Microstructures, Jiangsu Key Laboratory of Artificial Functional Materials, Nanjing University, Nanjing, 210023, China.

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http://dx.doi.org/10.1007/s11427-020-1937-6DOI Listing
June 2021

Synthesis-temperature-regulated multi-enzyme-mimicking activities of ceria nanozymes.

J Mater Chem B 2021 Jun 7. Epub 2021 Jun 7.

Department of Biomedical Engineering, College of Engineering and Applied Sciences, Nanjing National Laboratory of Microstructures, Jiangsu Key Laboratory of Artificial Functional Materials, Chemistry and Biomedicine Innovation Center (ChemBIC), Nanjing University, Nanjing, Jiangsu 210023, China. and State Key Laboratory of Analytical Chemistry for Life Science and State Key Laboratory of Coordination Chemistry, School of Chemistry and Chemical Engineering, Nanjing University, Nanjing, Jiangsu 210023, China.

Ceria (CeO2) nanozymes have drawn much attention in recent years due to their unique physiochemical properties and excellent biocompatibility. It is therefore very important to establish a simple and robust guideline to regulate CeO2 with desired multi-enzyme-mimicking activities that are ideal for practical bioapplications. In this work, the multi-enzyme-mimicking activities of CeO2 were regulated in a facile manner by a wet-chemical method with different synthesis temperatures. Interestingly, a distinct response in multi-enzyme-mimicking activities of CeO2 was observed towards different synthesis temperatures. And the regulation was ascribed to the comprehensive effect of the oxygen species, size, and self-restoring abilities of CeO2. This study demonstrates that high-performance CeO2 can be rationally designed by a specific synthesis temperature, and the guidelines from radar chart analysis established here can advance the biomedical applications of ceria-based nanozymes.
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http://dx.doi.org/10.1039/d1tb00964hDOI Listing
June 2021

TGFβ1 in Cancer-Associated Fibroblasts Is Associated With Progression and Radiosensitivity in Small-Cell Lung Cancer.

Front Cell Dev Biol 2021 20;9:667645. Epub 2021 May 20.

Department of Radiotherapy, Tianjin Medical University Cancer Institute and Hospital, Tianjin, China.

Objective: Small-cell lung cancer (SCLC) is aggressive, with early metastasis. Cytokines secreted by cancer-associated fibroblasts (CAFs) within various tumors influences these features, but the function in particular of TGFβ1 (transforming growth factor beta 1) is controversial and unknown in SCLC. This study explored the influence of TGFβ1 in CAFs on the development, immune microenvironment, and radiotherapy sensitivity of SCLC.

Methods: SCLC specimens were collected from 90 patients who had received no treatment before surgery. Tumor and tumor stroma were subjected to multiplex immunohistochemistry to quantitate TGFβ1 and other immune factors in CAFs. Cell proliferation and flow cytometry apoptosis assays were used to investigate associations between TGFβ1 and proliferation and radiotherapy sensitivity. The immune factors in tumors were detected by immunohistochemistry and (mice).

Results: TGFβ1 levels on CAFs lower or higher than the median were found, respectively, in 52.2 and 47.8% of patients; overall survival of patients with TGFβ1-high levels (53.9 mo) was significantly longer than that of the TGFβ1-low group (26.9 mo; = 0.037). The univariate and multivariate analyses indicated that a TGFβ1-high level was an independent predictor of increased survival time. TGFβ1-high levels in CAFs were associated with inhibition of growth, proliferation, antitumor immunity, and enhanced radiotherapeutic sensitivity and tumor immunity of tumor. TGFβ1-low levels promoted tumor cell growth and radiotherapy sensitivity and .

Conclusion: High levels of TGFβ1 in CAFs were associated with longer overall survival in patients with SCLC and enhanced radiotherapy sensitivity.
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http://dx.doi.org/10.3389/fcell.2021.667645DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8172974PMC
May 2021

Genome-Wide and Comprehensive Analysis of the Multiple Stress-Related CAF1 (CCR4-Associated Factor 1) Family and Its Expression in Poplar.

Plants (Basel) 2021 May 14;10(5). Epub 2021 May 14.

Co-Innovation Center for Sustainable Forestry in Southern China, Key Laboratory of Forest Genetics & Biotechnology, Ministry of Education, College of Biology and the Environment, Nanjing Forestry University, Nanjing 210037, China.

Poplar is one of the most widely used tree in afforestation projects. However, it is susceptible to abiotic and biotic stress. CCR4-associated factor 1 () is a major member of CCR4-NOT, and it is mainly involved in transcriptional regulation and mRNA degradation in eukaryotes. However, there are no studies on the molecular phylogeny and expression of the gene in poplar. In this study, a total of 19 genes were identified in the genome. Phylogenetic analysis of the gene family was performed with two closely related species ( and ) to investigate the evolution of the gene. The tissue expression of the gene showed that 19 genes were present in different tissues of poplar. Additionally, the analysis of the expression of the gene showed that the family was up-regulated to various degrees under biotic and abiotic stresses and participated in the poplar stress response. The results of our study provide a deeper understanding of the structure and function of the gene and may contribute to our understanding of the molecular basis of stress tolerance in poplar.
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http://dx.doi.org/10.3390/plants10050981DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8155972PMC
May 2021

PPAR Attenuates Interleukin-1-Induced Cell Apoptosis by Inhibiting NOX2/ROS/p38MAPK Activation in Osteoarthritis Chondrocytes.

Oxid Med Cell Longev 2021 4;2021:5551338. Epub 2021 May 4.

Department of Orthopedics, The Affiliated Changzhou No.2 People's Hospital of Nanjing Medical University, Changzhou 213003, China.

Introduction: Reactive oxygen species (ROS) induced by extracellular cytokines trigger the expression of inflammatory mediators in osteoarthritis (OA) chondrocyte. Peroxisome proliferator-activated receptor gamma (PPAR) exerts an anti-inflammatory effect. The aim of this study was to elucidate the role of PPAR in interleukin-1- (IL-1-) induced cyclooxygenase-2 (COX-2) and prostaglandin E (PGE) expression through ROS generation in OA chondrocytes.

Methods: IL-1-induced ROS generation and chondrocyte apoptosis were determined by flow cytometry. Contents of NADPH oxidase (NOX), caspase-3, and caspase-9 were evaluated by biochemical detection. The involvement of NOX2 and mitogen-activated protein kinases (MAPKs) in IL-1-induced COX-2 and PGE2 expression was investigated using pharmacologic inhibitors and further analyzed by western blotting. Activation of PPAR was performed by using a pharmacologic agonist and was analyzed by western blotting.

Results: IL-1-induced COX-2 and PGE expression was mediated through NOX2 activation/ROS production, which could be attenuated by N-acetylcysteine (NAC; a scavenger of ROS), GW1929 (PPAR agonist), DPI (diphenyleneiodonium chloride, NOX2 inhibitor), SB203580 (p38MAPK inhibitor), PD98059 (extracellular signal-regulated kinase, ERK inhibitor), and SP600125 (c-Jun N-terminal kinase, JNK inhibitor). ROS activated p38MAPK to enter the nucleus, which was attenuated by PPAR.

Conclusion: In OA chondrocytes, IL-1 induced COX-2 and PGE expression via activation of NOX2, which led to ROS production and MAPK activation. The activation of PPAR exerted protective roles in the pathogenesis of OA.
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http://dx.doi.org/10.1155/2021/5551338DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8112933PMC
May 2021

Influences of temperature and moisture on abiotic and biotic soil CO emission from a subtropical forest.

Carbon Balance Manag 2021 May 25;16(1):18. Epub 2021 May 25.

College of Natural Resources and Environment, South China Agricultural University, Guangzhou, 510483, China.

Background: Soil CO efflux is considered to mainly derive from biotic activities, while potential contribution of abiotic processes has been mostly neglected especially in productive ecosystems with highly active soil biota. We collected a subtropical forest soil to sterilize for incubation under different temperature (20 and 30 °C) and moisture regimes (30%, 60 and 90% of water holding capacity), aiming to quantify contribution of abiotic and biotic soil CO emission under changing environment scenarios.

Main Findings: Results showed that abiotic processes accounted for a considerable proportion (15.6-60.0%) of CO emission in such a biologically active soil under different temperature and moisture conditions, and the abiotic soil CO emission was very likely to derive from degradation of soil organic carbon via thermal degradation and oxidation of reactive oxygen species. Furthermore, compared with biotically driving decomposition processes, abiotic soil CO emission was less sensitive to changes in temperature and moisture, causing reductions in proportion of the abiotic to total soil CO emission as temperature and moisture increased.

Conclusions: These observations highlight that abiotic soil CO emission is unneglectable even in productive ecosystems with high biological activities, and different responses of the abiotic and biotic processes to environmental changes could increase the uncertainty in predicting carbon cycling.
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http://dx.doi.org/10.1186/s13021-021-00181-8DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8152076PMC
May 2021

A Computational Model of Working Memory Based on Spike-Timing-Dependent Plasticity.

Front Comput Neurosci 2021 21;15:630999. Epub 2021 Apr 21.

Laboratory of Cognitive Model and Algorithm, Shanghai Key Laboratory of Data Science, Department of Computer Science, Fudan University, Shanghai, China.

Working memory is closely involved in various cognitive activities, but its neural mechanism is still under exploration. The mainstream view has long been that persistent activity is the neural basis of working memory, but recent experiments have observed that activity-silent memory can also be correctly recalled. The underlying mechanism of activity-silent memory is considered to be an alternative scheme that rejects the theory of persistent activity. We propose a working memory model based on spike-timing-dependent plasticity (STDP). Different from models based on spike-rate coding, our model adopts temporal patterns of action potentials to represent information, so it can flexibly encode new memory representation. The model can work in both persistent and silent states, i.e., it is compatible with both of these seemingly conflicting neural mechanisms. We conducted a simulation experiment, and the results are similar to the real experimental results, which suggests that our model is plausible in biology.
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http://dx.doi.org/10.3389/fncom.2021.630999DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8096998PMC
April 2021

Sex-based differences in fecal short-chain fatty acid and gut microbiota in irritable bowel syndrome patients.

J Dig Dis 2021 May;22(5):246-255

Department of Gastroenterology, Peking University Third Hospital, Beijing, China.

Objective: To explore alterations in fecal short-chain fatty acids (SCFA) and gut microbiota in patients with diarrhea-predominant irritable bowel disease (IBS-D) and their relationships with clinical manifestations.

Methods: We recruited 162 patients with IBS-D and 66 healthy controls (HC). Their manifestations and psychological status were evaluated using the IBS severity scoring system and the Hospital Anxiety and Depression Scale (HADS). Colorectal visceral sensitivity was evaluated using a barostat. Systemic inflammation was evaluated using plasma cytokine levels. Fecal SCFA were quantified using ultra-performance liquid chromatography-tandem mass spectrometry, and fecal microbiota communities were analyzed using 16S rRNA sequencing.

Results: More men presented with IBS-D than women in our patient cohort. Patients with IBS-D had more severe manifestations, higher HADS score, and a higher rate of previous infectious enteritis than HC. Notably, female patients had significantly higher HADS scores than male patients. Male patients had significantly higher levels of plasma interleukin (IL)-12, fecal propionate and colorectal visceral sensitivity than male HC, while no differences were observed between female patients and female HC. Fecal acetate, butyrate and valerate correlated with the initial visceral sensory threshold, stressors, and IL-10 and IL-12 levels. The propionate-producing Prevotella 9 genus was significantly increased in male patients and positively correlated with fecal propionate.

Conclusion: Distinct sex-based differences in clinical manifestations, fecal SCFA and microbiota richness are found in Chinese patients with IBS-D, which may be used to diagnose dysbiosis in these patients.
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http://dx.doi.org/10.1111/1751-2980.12988DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8252430PMC
May 2021

Phase Tailoring of Ruddlesden-Popper Perovskite at Fixed Large Spacer Cation Ratio.

Small 2021 Apr 4:e2100560. Epub 2021 Apr 4.

Joint Key Laboratory of the Ministry of Education, Institute of Applied Physics and Materials Engineering, University of Macau, Avenida da Universidade, Taipa, Macao, 999078, China.

Ruddlesden-Popper (RP) metal halide perovskites are considered as promising optoelectronic materials due to their good environmental stability and desirable optoelectronic properties. However, the phase composition and ordering in the deposited film, with a fixed ratio of large organic spacer cation in the precursor solution, are hard to be further tailored for specific optoelectronic applications. Herein, it is shown that even with a fixed spacer cation ratio, the phase composition and ordering can still be largely regulated by utilizing different crystallization kinetics of various cations with the inorganic octahedral lead halide. By using two different short cations to compete with the large spacer cation, the phase composition can be continuously tailored from thin multiple quantum wells (MQWs) dominated to 3D perovskite dominated. The phase ordering can be reversed from small n phases' prior to large n phases' prior near the substrate. Finally, with the same amount of large spacer cation protection, the perovskite can be tailored for both high-performance electroluminescence and photovoltaics with favorable energetic landscape for the corresponding desired first-order excitonic recombination and second-order free electron-hole recombination, respectively. This exploration substantially contributes to the understanding of precise phase engineering in RP perovskite and may provide a new insight into the design of multiple functional devices.
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http://dx.doi.org/10.1002/smll.202100560DOI Listing
April 2021

Reduced pests, improved grain quality and greater total income: benefits of intercropping rice with Pontederia cordata.

J Sci Food Agric 2021 Apr 4. Epub 2021 Apr 4.

Guangdong Provincial Key Laboratory of Eco-Circular Agriculture, South China Agricultural University, Guangzhou, P. R. China.

Background: Intercropping, which is growing two or more different crops in the same field simultaneously, is an effective traditional agricultural practice for productivity, resource utilization, and pest control. However, study on intercropping in paddy fields is limited. So in this study, field experiments of 2 years/four seasons (early and late seasons in 2016 and 2017) were conducted to examine the effects of rice-Pontederia cordata intercropping on rice plant growth, pest control, yield, income, and grain quality.

Results: We found rice-P. cordata intercropping significantly decreased the occurrence of rice diseases and pests, with a 22.0-45.9% reduction in sheath blight and a 33.8-34.4% reduction in leaf folders. The mean land equivalent ratio (LER) (1.09) result indicates that intercropping rice and P. cordata generated positive yield effects. In addition, due to the economic profit from the replacement stripe of P. cordata in the rice paddy field, intercropping rice with P. cordata could greatly enhance farmer income. The average total income of rice intercropped with P. cordata was 2.5-fold higher than that of rice monoculture. Furthermore, intercropping significantly improved grain quality compared with the rice monoculture. It significantly increased the milled rice rate and whole milled rice rate by 11.2% and 12.8%, respectively, but decreased the chalky rice rate by 30.9-39.8% and chalkiness degree by 32.2%.

Conclusions: We suggest that rice-P. cordata intercropping provides an environmentally effective way to control rice diseases and pests, results in higher overall productivity and total income, and improves grain quality. © 2021 Society of Chemical Industry.
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http://dx.doi.org/10.1002/jsfa.11243DOI Listing
April 2021

Exsolution of Noble-Metal Nanoparticles on Perovskites as Enhanced Peroxidase Mimics for Bioanalysis.

Anal Chem 2021 04 2;93(14):5954-5962. Epub 2021 Apr 2.

Department of Biomedical Engineering, College of Engineering and Applied Sciences, Nanjing National Laboratory of Microstructures, Jiangsu Key Laboratory of Artificial Functional Materials, Nanjing University, Nanjing, Jiangsu 210023, China.

Various transition-metal oxide (TMO)-based nanomaterials have been explored as peroxidase mimics. However, the moderate peroxidase-like activity of TMOs limited their widespread use. Decorating highly active noble-metal nanozymes on the surface of TMOs can not only enhance the peroxidase-like activity of TMOs but also prevent the small-sized metal nanoparticles (NPs) from aggregation. Herein, exsolution of noble-metal NPs (i.e., Ir and Ru) from A-site-deficient perovskite oxides (i.e., chemical formula LaBB'O, B = Mn/Fe, B' = Ir/Ru) under a reducing atmosphere was achieved for preparing noble-metal NPs/perovskite composites. The exsolved NPs were socketed on the surface of parent perovskite oxides, which significantly enhanced the stability of metal NPs. In addition, the peroxidase-like activity of perovskite oxides increased remarkably after NPs egress. We then used the optimized Ir/LMIO with high stability and excellent peroxidase-like activity to develop a colorimetric assay for the determination of alkaline phosphatase (ALP). Benefiting from the remarkable peroxidase-like activity of Ir/LMIO, the sensing platform exhibited a wide linear range. The practical application of the colorimetric sensing method was demonstrated by detecting the ALP in serum samples. This work not only provides new insights into the synthesis of highly active peroxidase-like nanozymes but expands their applications for constructing a high-performance biosensing platform.
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http://dx.doi.org/10.1021/acs.analchem.1c00721DOI Listing
April 2021

Stabilizing black-phase formamidinium perovskite formation at room temperature and high humidity.

Science 2021 03;371(6536):1359-1364

Key Laboratory of Flexible Electronics (KLoFE) and Institute of Advanced Materials (IAM), Nanjing Tech University, 30 South Puzhu Road, Nanjing 211816, Jiangsu, China.

The stabilization of black-phase formamidinium lead iodide (α-FAPbI) perovskite under various environmental conditions is considered necessary for solar cells. However, challenges remain regarding the temperature sensitivity of α-FAPbI and the requirements for strict humidity control in its processing. Here we report the synthesis of stable α-FAPbI, regardless of humidity and temperature, based on a vertically aligned lead iodide thin film grown from an ionic liquid, methylamine formate. The vertically grown structure has numerous nanometer-scale ion channels that facilitate the permeation of formamidinium iodide into the lead iodide thin films for fast and robust transformation to α-FAPbI A solar cell with a power-conversion efficiency of 24.1% was achieved. The unencapsulated cells retain 80 and 90% of their initial efficiencies for 500 hours at 85°C and continuous light stress, respectively.
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http://dx.doi.org/10.1126/science.abf7652DOI Listing
March 2021

Three-year-period nitrogen additions did not alter soil organic carbon content and lability in soil aggregates in a tropical forest.

Environ Sci Pollut Res Int 2021 Jul 15;28(28):37793-37803. Epub 2021 Mar 15.

Key Laboratory of Vegetation Restoration and Management of Degraded Ecosystems, South China Botanical Garden, Chinese Academy of Sciences, Guangzhou, 510650, China.

Soil immobilizes a considerable proportion of carbon (C) as organic matter in terrestrial ecosystems and is thus critical to stabilize the global climate system. Atmospheric nitrogen (N) deposition could influence soil C storage and stabilization, but how N deposition changes soil organic C (SOC) fractions and lability remains elusive. We investigated the effects of 3-year-period N inputs on SOC fractions and lability along three soil depths (0-10, 10-20, and 20-40 cm) in a tropical forest of southern China. Results showed that N additions did not significantly change contents of SOC fractions and the C lability, either in bulk or aggregate-based soils at any of the three depths, and it showed no significant interaction with soil aggregate or soil depth. The SOC content was 43.7 ± 1.5, 18.2 ± 1.0, and 10.7 ± 0.4 mg g at the three soil layers downwards, with the non-readily oxidizable SOC (NROC) contributing over 70% while the remaining SOC consisting of readily oxidizable SOC at each soil layer. Moreover, contents of SOC and NROC were consistently higher in small soil aggregates, but the C decrement with increasing size of soil aggregates declined along soil profile downwards. This scenario suggests that physical protection of the small soil aggregate is limited, but its greater specific surface area could obviously contribute to the SOC pattern among soil aggregates. These results indicate that the highly developed forests could be resistant to short-term N deposition, even with a high load, to maintain its SOC stabilization.
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http://dx.doi.org/10.1007/s11356-021-13466-9DOI Listing
July 2021

Auer rods in mixed phenotype acute leukemia, T/myeloid: A report of three cases.

Leuk Res Rep 2021 9;15:100236. Epub 2021 Feb 9.

State Key Laboratory of Experimental Hematology and National Clinical Research Center for Blood Diseases, Institute of Hematology and Blood Disease Hospital, Chinese Academy of Medical Sciences, Peking Union Medical College, Tianjin, China.

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http://dx.doi.org/10.1016/j.lrr.2021.100236DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7902533PMC
February 2021

Iron incorporation both intra- and extra-cellularly improves the yield and saccharification of switchgrass (Panicum virgatum L.) biomass.

Biotechnol Biofuels 2021 Mar 4;14(1):55. Epub 2021 Mar 4.

Biosciences Center, National Renewable Energy Laboratory, Golden, CO, 80401, USA.

Background: Pretreatments are commonly used to facilitate the deconstruction of lignocellulosic biomass to its component sugars and aromatics. Previously, we showed that iron ions can be used as co-catalysts to reduce the severity of dilute acid pretreatment of biomass. Transgenic iron-accumulating Arabidopsis and rice plants exhibited higher iron content in grains, increased biomass yield, and importantly, enhanced sugar release from the biomass.

Results: In this study, we used intracellular ferritin (FerIN) alone and in combination with an improved version of cell wall-bound carbohydrate-binding module fused iron-binding peptide (IBPex) specifically targeting switchgrass, a bioenergy crop species. The FerIN switchgrass improved by 15% in height and 65% in yield, whereas the FerIN/IBPex transgenics showed enhancement up to 30% in height and 115% in yield. The FerIN and FerIN/IBPex switchgrass had 27% and 51% higher in planta iron accumulation than the empty vector (EV) control, respectively, under normal growth conditions. Improved pretreatability was observed in FerIN switchgrass (~ 14% more glucose release than the EV), and the FerIN/IBPex plants showed further enhancement in glucose release up to 24%.

Conclusions: We conclude that this iron-accumulating strategy can be transferred from model plants and applied to bioenergy crops, such as switchgrass. The intra- and extra-cellular iron incorporation approach improves biomass pretreatability and digestibility, providing upgraded feedstocks for the production of biofuels and bioproducts.
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http://dx.doi.org/10.1186/s13068-021-01891-4DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7931346PMC
March 2021

Inorganic Enzyme Mimics.

Chembiochem 2021 May 4;22(9):1496-1498. Epub 2021 Mar 4.

Department of Biomedical Engineering, College of Engineering and Applied Sciences, Nanjing National Laboratory of Microstructures, Jiangsu Key Laboratory of Artificial Functional Materials, Nanjing University, Nanjing, Jiangsu, 210023, P. R. China.

Enzyme mimics (or artificial enzymes) have emerged as valuable alternatives to natural enzymes since the pioneering work of Ronald Breslow. They have numerous advantages over natural enzymes, such as high stability, low cost, and tailorable activity. Among varieties of materials explored to mimic enzymes, the inorganic ones, including inorganic complexes and nanomaterials, have attracted increasing interest over the last decade and have the potential to address the current challenges in energy, environment, health, etc.
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http://dx.doi.org/10.1002/cbic.202100049DOI Listing
May 2021

Activities of daily living measurement after ischemic stroke: Rasch analysis of the modified Barthel Index.

Medicine (Baltimore) 2021 Mar;100(9):e24926

Nursing Department, the Second Affiliated Hospital of Zhejiang University School of Medicine, Hangzhou 310009, China.

Abstract: In patients with ischemic stroke, activities of daily living were used as an outcome indicator, and correct assessment is very important. We sought to examine the reliability and validity of the modified Barthel Index as an evaluation tool of activities of daily living in ischemic stroke patients by applying the Rasch analysis.We used a prospectively collected cohort of ischemic stroke patients in the department of neurology. Rasch analysis was used for evaluating the reliability and validity of the modified Barthel Index.A total of 231 patients were included in the analysis. The average of modified Barthel Index was 36.2 ± 17.8. The modified Barthel Index had high reliability of 0.88. There were no extremely mismatched items, and considered unidimensional, but the Point-Measure of bowels and bladder were 0.27, extremely lower than other items. The scale was stable in different sex and age, but had notable differential item functioning in muscle strength of the limbs. Rating categories were not functioning adequately in items. The item difficulty and patient ability were not matched, with a difference of 1.17 logics. 29.4% patients, no easy items could match their ability.The modified Barthel Index had high reliability but a relatively bad matching degree between item difficulty and patient ability. It still needs further improvement to reflect the activities of daily living in ischemic stroke patients.
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http://dx.doi.org/10.1097/MD.0000000000024926DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7939171PMC
March 2021

Increased Expression of Colonic Mucosal Melatonin in Patients with Irritable Bowel Syndrome Correlated with Gut Dysbiosis.

Genomics Proteomics Bioinformatics 2021 Feb 17. Epub 2021 Feb 17.

Department of Gastroenterology, Peking University Third Hospital, Beijing 100191, China. Electronic address:

Dysregulation of the gut microbiota/gut hormone axis contributes to the pathogenesis of irritable bowel syndrome (IBS). Melatonin plays a beneficial role in gut motility and immunity. However, altered expression of local mucosal melatonin in IBS and its relationship with the gut microbiota remain unclear. Therefore, we aimed to detect the colonic melatonin levels and microbiota profiles in patients with diarrhea-predominant IBS (IBS-D) and explore their relationship in germ-free (GF) rats and BON-1 cells. Thirty-two IBS-D patients and twenty-eight healthy controls (HCs) were recruited. Fecal specimens from IBS-D patients and HCs were separately transplanted into GF rats by gavage. The levels of colon mucosal melatonin were assessed by immunohistochemical methods, and fecal microbiota communities were analyzed using 16S rDNA sequencing. The effect of butyrate on melatonin synthesis in BON-1 cells was evaluated by ELISA. Melatonin levels were significantly increased and negatively correlated with visceral hypersensitivity in IBS-D patients. GF rats inoculated with fecal microbiota from IBS-D patients had high colonic melatonin levels. Butyrate-producing Clostridium cluster XIVa species, such as Roseburia species and Lachnospira species, were positively related to colonic mucosal melatonin expression. Butyrate significantly increased melatonin secretion in BON-1 cells. Increased melatonin expression may be an adaptive protective mechanism in the development of IBS-D. Moreover, some Clostridium cluster XIVa species could increase melatonin expression via butyrate production. Modulation of the gut hormone/gut microbiota axis offers a promising target of interest for IBS in the future.
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http://dx.doi.org/10.1016/j.gpb.2020.06.013DOI Listing
February 2021

Downregulation of IRAK3 by miR-33b-3p relieves chondrocyte inflammation and apoptosis in an in vitro osteoarthritis model.

Biosci Biotechnol Biochem 2021 Feb;85(3):545-552

Department of Orthopedics, The Affiliated Changzhou No. 2 People's Hospital of Nanjing Medical University, Changzhou, Jiangsu, China.

Interleukin-1 receptor-associated kinase-3 (IRAK3) has a distinctive role in regulating inflammation. However, the functional role of IRAK3 and regulatory mechanism underlying the pathogenesis of osteoarthritis (OA) remain unclear. Here, we first found that IRAK3 was upregulated, while miR-33b-3p was downregulated in the cartilage of OA patients and IL-1β-induced CHON-001 cells. IRAK3 was confirmed as the direct target of miR-33b-3p and negatively regulated by miR-33b-3p. There was an inverse correlation between IRAK3 mRNA expression and miR-33b-3p expression in OA cartilage tissues. The in vitro functional experiments showed that miR-33b-3p overexpression caused a remarkable increase in viability, a significant decrease in inflammatory mediators (IL-1β and TNF-α), and apoptosis in IL-1β-induced CHON-001 cells. Importantly, IRAK3 knockdown imitated, while overexpression reversed the effects of miR-33b-3p on IL-1β-induced inflammation and apoptosis in CHON-001 cells. Collectively, miR-33b-3p significantly alleviated IL-1β-induced inflammation and apoptosis by downregulating IRAK3, which may serve as a promising target for OA.
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http://dx.doi.org/10.1093/bbb/zbaa105DOI Listing
February 2021

Optimized clinical application of minimal residual disease in acute myeloid leukemia with RUNX1-RUNX1T1.

Exp Hematol 2021 Apr 29;96:63-72.e3. Epub 2021 Jan 29.

State Key Laboratory of Experimental Hematology, Institute of Hematology and Blood Diseases Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Tianjin, China; National Clinical Research Center for Blood Disease, Tianjin, China; Leukemia Center, Institute of Hematology and Blood Diseases Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Tianjin, China. Electronic address:

Minimal residual disease (MRD) levels monitored by polymerase chain reaction are associated with outcomes in acute myeloid leukemia with RUNX1-RUNX1T1. The objectives of our study were to quantitatively compare the predictive value of MRD reduction and absolute copies and assess the influence of other prognostic factors on MRD. A total of 224 consecutive patients with RUNX1-RUNX1T1 aged ≤55 years were included in the MRD study. Patients received different induction regimens including conventional- or intermediate-dose cytarabine plus low-dose daunorubicin and omacetaxine mepesuccinate or daunorubicin at 60 mg/m/day on days 1-3. As continuous variables, both MRD reduction and absolute MRD level were significantly associated with cumulative incidence of relapse (CIR; hazard ratio [HR] = 1.610, 95% confidence interval [CI]: 1.370-1.890, p < 0.001, and HR = 1.170, 95% CI: 1.120-1.230, p < 0.001, respectively). For the CIR, the area under the curves (AUCs) of MRD reduction and absolute MRD level after the first consolidation chemotherapy were 0.629 and 0.629, respectively. Intermediate-dose cytarabine induction (HR = 0.494; p = 0.039 for CIR, HR, 0.451; p = 0.014 for RFS, and HR, 0.262; p = 0.006 for OS) remained significantly associated with outcomes after adjusting for MRD reduction after the first consolidation therapy (HR = 1.456, p < 0.001, for CIR; HR = 1.467, p = 0.001, for relapse-free survival; and HR = 1.468, p = 0.014, for overall survival) in multivariate analyses. In conclusion, the prognostic significance of MRD after the first consolidation therapy was influenced by the induction regimen in acute myeloid leukemia with RUNX1-RUNX1T1.
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http://dx.doi.org/10.1016/j.exphem.2021.01.007DOI Listing
April 2021

In vitro measurement of superoxide dismutase-like nanozyme activity: a comparative study.

Analyst 2021 Mar 1;146(6):1872-1879. Epub 2021 Feb 1.

Department of Biomedical Engineering, College of Engineering and Applied Sciences, Nanjing University, Nanjing, Jiangsu 210023, China.

Analyzing the SOD-like activity of nanozymes in vitro is of great importance for identifying new nanozymes and predicting their potential biological effects in vivo. However, false negative or positive results occasionally occur due to the mismatch between the detection methods and nanozymes. Here, five typical SOD-like nanozymes, including CeO, MnO, Prussian blue (PB), PCN222-Mn, and Pt NPs, have been used to evaluate the sensitivity and accuracy of several commonly used in vitro detection methods. By systematically analyzing the detection results, several precautions have been taken. (1) The hydroethidine (HE) probe could be disturbed by the nanozyme with oxidative ability. (2) The nitro blue tetrazolium (NBT) probe has a moderate sensitivity due to the poor water solubility of its reduced product. (3) The water-soluble tetrazolium salt (WST)-8 probe has a higher sensitivity than both NBT and iodonitrotetrazolium chloride (INT). (4) The detection system using the irradiation of riboflavin to produce ˙O might be interfered by the nanozyme with photosensibility. (5) Both the quality of DMPO and incubation time are important factors for electron paramagnetic resonance (EPR) measurement. This study will be useful for choosing more suitable in vitro detection methods of SOD-like activity for nanozymes in the future.
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http://dx.doi.org/10.1039/d0an02164dDOI Listing
March 2021

Correction to: Clinical characteristics of 14 COVID-19 deaths in Tianmen, China: a single-center retrospective study.

BMC Infect Dis 2021 Jan 27;21(1):119. Epub 2021 Jan 27.

Division of Rheumatology, The First People's Hospital of Tianmen, Tianmen, Hubei, China.

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http://dx.doi.org/10.1186/s12879-021-05835-zDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7839287PMC
January 2021