Publications by authors named "Hui Su"

220 Publications

Clinical significance of combined detection of anti-Mullerian hormone and follicular output rate in women of late reproductive age.

Am J Transl Res 2021 15;13(6):6270-6278. Epub 2021 Jun 15.

Department of Reproductive Medicine, Hengshui People's Hospital Hengshui 053000, Hebei Province, China.

Objective: This study was designed to explore the clinical significance of anti-Mullerian hormone (AMH) combined with follicular output rate (FORT) in women of late reproductive age.

Methods: A total of 258 women (age range: 35-45 years old) who underwent pre-pregnancy examination in our hospital were collected as the research group (RG), among whom 184 were treated with fertilization-embryo transfer (IVF-ET). Concurrently, 126 women aged 24-30 years who came to our hospital for pre-pregnancy examination were enrolled as the control group (CG). AMH and FORT were detected and compared between the two groups to analyze the clinical significance of the two in women of late reproductive age.

Results: Compared with the CG, AMH was decreased statistically in the RG (P<0.05). AMH was statistically higher in the regular menstrual group than in the menstrual disorder group (P<0.05), and FORT was statistically higher in the pregnancy group in comparison with the non-pregnancy group (P<0.05). AMH decreased with age (P<0.05), while FORT did not correlate with any notable difference among the three subgroups (P>0.05). High, medium and low AMH groups showed no significant difference in the number of retrieved oocytes and transplantable embryos, as well as FORT (P<0.05). A lower AMH level, was correlated with fewer number of retrieved oocytes and transplantable embryos, and higher the FORT level. Significant differences were present among the high, middle and low FORT groups regarding the number of retrieved oocytes and transplantable embryos, the clinical pregnancy rate and AMH level (P<0.05). The lower the level of FORT was, the less the number of retrieved oocytes and transplantable embryos was, the lower clinical pregnancy rate was, and the higher the AMH level was.

Conclusions: AMH decreases gradually in women with an increase of age, and FORT can effectively predict pregnancy outcome. AMH detection combined FORT is of great significance in predicting the ovarian reserve function in women of late reproductive age.
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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8290787PMC
June 2021

The feasibility of family-centred early intervention for children with disabilities in mainland China: Practitioners' perceptions.

Child Care Health Dev 2021 Jul 15. Epub 2021 Jul 15.

Division of Vocational Education, Shenzhen Yuanping Special School, Shenzhen, China.

Background: Family-centred practice (FCP) has become a recommended practice for early intervention services for children with disabilities in many countries. However, its feasibility in Chinese context has been unclear. This study is the first to explore the perceptions of early intervention service practitioners about the implementation of FCP in mainland China.

Methods: Focus groups were employed to collect data from 37 early intervention practitioners who attended a workshop about FCP in Wuhan, China and two officers from the provincial disabled persons' federation. The data were analysed thematically.

Results: Four themes were identified: (a) family-centred early intervention is possible, (b) traditional concepts are not friendly towards FCP, (c) parents do not collaborate and (d) financing and personal resources are not sufficient to implement FCP. Chinese practitioners agreed with the philosophies of FCP; however, there was concern that widespread implementation may meet conceptual and practical challenges.

Conclusions: The results highlighted practitioners were optimistic and keen for FCP implementation in the Chinese context, but to do so across China may still be some way in the future.
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http://dx.doi.org/10.1111/cch.12898DOI Listing
July 2021

Normal Values of High-resolution Manometry Parameters With Provocative Maneuvers.

J Neurogastroenterol Motil 2021 Jul;27(3):354-362

Division of Gastroenterology and Hepatology, Department of Medicine, Feinberg School of Medicine, Northwestern University, Chicago, IL, USA.

Background/aims: Incorporation of complementary and provocative test swallows to the high-resolution manometry (HRM) protocol offers potential to address limitations posed by HRM protocols that involve only a single swallow type. The aim of this study is to describe normal findings of a comprehensive HRM testing protocol performed on healthy asymptomatic volunteers.

Methods: Thirty healthy asymptomatic volunteers completed HRM with 5-mL liquid swallows in the supine position. They also completed 5-mL liquid swallows in the upright position, viscous swallows, solid test swallows, multiple rapid swallows, and a rapid drink challenge. HRM studies were analyzed via Chicago classification version 3.0.

Results: The median (5th-95th percentiles) for integrated relaxation pressure (IRP) on supine swallows was 11 (4-16) mmHg; IRP was lower than supine on upright liquid 9 (0-17) mmHg, viscous 6 (0-15) mmHg, solid 9 (1-19) mmHg, multiple rapid swallows 3 (0-12) mmHg, and rapid drink challenge 5 (-3-12) mmHg; < 0.005. While an "elevated" IRP value was observed on 1 to 2 test maneuvers in 8/30 (27%) subjects, all 30 subjects had an IRP value < 12 mmHg on at least one of the test maneuvers.

Conclusions: Normal values and findings from a comprehensive HRM testing protocol are reported based on evaluation of 30 healthy asymptomatic volunteers. Isolated "abnormalities" of IRP and contractile parameters were observed in the majority (80%) of these asymptomatic subjects, while all subjects also had normal features observed. Thus, the definition of "normal" should be recalibrated to focus on the entirety of the study and not individual metrics.
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http://dx.doi.org/10.5056/jnm20118DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8266507PMC
July 2021

Exploration of the effect of mixed probiotics on microbiota of allergic asthma mice.

Cell Immunol 2021 Jun 24;367:104399. Epub 2021 Jun 24.

Department of Pediatrics, Xijing Hospital, the Fourth Military Medical University, Xi'an 710032, China. Electronic address:

Co-relation between allergic asthma and microbiota varying with diet has been extensively investigated, implicating that oral supplement of alternative pharmaceuticals are potential for asthma control. Probiotics are of great concern due to its beneficial effects on the host, whereas the potential mechanisms and the optimal dose need to be further explored. In the present study, three different doses of mixed strains were given orally to mouse model of allergic asthma induced by ovalbumin (OVA). Continuous administration of mixed probiotics could alleviate OVA-induced allergic inflammation through reducing costimulatory molecules on the surface of dendritic cells (DCs) and increasing mucosal gut-primed Tregs induced by mesenteric CD103DCs. Mixed probiotics-induced protective effect relates to gut microbiota instead of lung microbes. Microbial diversity and Bacteroidetes/Firmicutes (B/F) ratio are augmented upon probiotic strains. Interestingly, treatment with mixed strain lead to an increased levels of genus Lactobacillus. Lactobacillus genus-Operational Taxonomic Unit (OUT) analysis indicated that OTU9 may be the dominant strain from mixed probiotics providing protective effect. The low dose seems to be the best in the whole study. Our results provide new evidence for probiotic application in allergic diseases and support the idea that targeted gut microbiota will be an effective approach for allergic airway diseases.
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http://dx.doi.org/10.1016/j.cellimm.2021.104399DOI Listing
June 2021

Electrochemical activation of C-H by electron-deficient WC nanocrystals for simultaneous alkoxylation and hydrogen evolution.

Nat Commun 2021 Jun 23;12(1):3882. Epub 2021 Jun 23.

School of Chemistry and Chemical Engineering, Shanghai Jiao Tong University, Shanghai, China.

The activation of C-H bonds is a central challenge in organic chemistry and usually a key step for the retro-synthesis of functional natural products due to the high chemical stability of C-H bonds. Electrochemical methods are a powerful alternative for C-H activation, but this approach usually requires high overpotential and homogeneous mediators. Here, we design electron-deficient WC nanocrystal-based electrodes to boost the heterogeneous activation of C-H bonds under mild conditions via an additive-free, purely heterogeneous electrocatalytic strategy. The electron density of WC nanocrystals is tuned by constructing Schottky heterojunctions with nitrogen-doped carbon support to facilitate the preadsorption and activation of benzylic C-H bonds of ethylbenzene on the WC surface, enabling a high turnover frequency (18.8 h) at a comparably low work potential (2 V versus SCE). The pronounced electron deficiency of the WC nanocatalysts substantially facilitates the direct deprotonation process to ensure electrode durability without self-oxidation. The efficient oxidation process also boosts the balancing hydrogen production from as-formed protons on the cathode by a factor of 10 compared to an inert reference electrode. The whole process meets the requirements of atomic economy and electric energy utilization in terms of sustainable chemical synthesis.
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http://dx.doi.org/10.1038/s41467-021-24203-8DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8222219PMC
June 2021

C1q/TNF-related protein-9 ameliorates hypoxia-induced pulmonary hypertension by regulating secretion of endothelin-1 and nitric oxide mediated by AMPK in rats.

Sci Rep 2021 May 31;11(1):11372. Epub 2021 May 31.

Department of Pediatrics, Xijing Hospital, Fourth Military Medical University, 127 Changlexi Road, Xi'an, 710032, China.

Injury/dysfunction of the endothelium of pulmonary arteries contributes to hypoxia-induced pulmonary hypertension (HPH). We investigated whether C1q/tumor necrosis factor-related protein-9 (CTRP9), a newly identified cardiovascular agent, has protective roles in the development of HPH. HPH was induced in adult male rats by chronic hypobaric hypoxia. CTRP9 overexpression by adeno-associated virus (AAV)-CTRP9 transfection attenuated the increases in right ventricular systolic pressure, right ventricular hypertrophy index, and pulmonary arterial remodeling of rats under hypoxia. Importantly, CTRP9 overexpression improved endothelium-dependent vasodilation in pulmonary arterioles in HPH rats. CTRP9 overexpression enhanced expression of phosphorylated 5'-adenosine monophosphate-activated protein kinase (p-AMPK) and phosphorylated endothelial nitric oxide synthase (p-eNOS), and reduced phosphorylated extracellular signal-regulated protein kinase (p-ERK1/2) expression in pulmonary microvascular endothelial cells (PMVECs) of HPH rats. In cultured PMVECs, CTRP9 not only preserved the decrease of AMPK and eNOS phosphorylation level and nitric oxide (NO) production induced by hypoxia, but also blocked the increase in hypoxia-induced ERK1/2 phosphorylation level and endothelin (ET)-1 production. Furthermore, the effects of CTRP9 were interrupted by inhibitors or knockdown of AMPK. CTRP9 enhances NO production and reduces ET-1 production by regulating AMPK activation. CTRP9 could be a target for HPH.
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http://dx.doi.org/10.1038/s41598-021-90779-2DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8166879PMC
May 2021

Effects of the Vertebral Artery Ostium/Subclavian Artery Angle on In-Stent Restenosis after Vertebral Artery Ostium Stenting.

Biomed Res Int 2021 27;2021:5527988. Epub 2021 Apr 27.

Department of Neurology, Chinese PLA General Hospital, First Medical Center, Beijing, China.

Methods: Between January 2016 and October 2018, sixty-four consecutive patients who underwent a total of 66 stenting procedures were screened for symptomatic and asymptomatic atherosclerotic VAOS. Of these patients, 57 had complete follow-up data. The baseline patient demographics and morphological features of the VAO were recorded. Potential factors influencing ISR, including conventional cerebrovascular disease risk factors, were assessed, together with outcome events including recurrent transient ischemic attack (TIA), stroke, and vascular-related mortality.

Results: The average follow-up period was 13.2 ± 4.6 months. Technical success was achieved in all interventions. The degree of stenosis was reduced from 77.2 ± 6.1% to 13.7 ± 8.9% after the procedure. ISR was detected in eight treated vessels (14.0%) and occlusion in two (5.3%) arteries. Of the 57 patients, one had an ischemic stroke and 5 had TIAs. The angle of the VAO at the subclavian artery was associated with the risk of restenosis (preoperative, = 0.04; postoperative, = 0.02).

Conclusions: Stenting is a feasible and effective treatment for VAOS. The angle of the VAO at the subclavian artery may contribute to the development of ISR.
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http://dx.doi.org/10.1155/2021/5527988DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8101481PMC
June 2021

Phylogenetic characteristics of HIV among female cross-border travelers in Yunnan province between 2003 and 2012.

J Med Virol 2021 Aug 24;93(8):5173-5176. Epub 2021 May 24.

Clinical lab, Care Center for International Travel Health in Yunnan, Kunming, Yunnan, China.

Sexual transmission is currently the main mode of transmission of the human immunodeficiency virus (HIV). In this study, 181 HIV-infected female cross-border travelers entering Yunnan province were recruited between 2003 and 2012. HIV RNAs were extracted from their frozen serum and gag-pol gene sequences were obtained for phylogenetic and recombination analyses. In total, 131 gag-pol gene sequences were obtained successfully, at a rate of 72.4%. The most prevalent subtypes were CRF01_AE, followed by CRF08_BC, subtypes B and C. The other four subjects were classified as undefined subtypes and other recombinants. The subtype distribution of intravenous drug users was significantly different from that of sexually transmitted infections and unknown groups. The genetic distances of subtype B, C, and CRF01_AE strains were all close to the reference sequences from Yunnan province and Southeast Asian countries. Gene diversity and cocirculation of multiple subtypes were observed in female cross-border travelers, and CRF01_AE was the dominant epidemic subtype. The advantages of these subtype preferences for sexual transmission were obvious in HIV infection and transmission among this population. Our findings also suggest that close attention should be given to the HIV infection status of the female migrant population. In addition, a description of their epidemic characteristics is significant for the surveillance and prevention of acquired immunodeficiency syndrome in the Yunnan province.
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http://dx.doi.org/10.1002/jmv.27079DOI Listing
August 2021

Low Serum LH Levels During Ovarian Stimulation With GnRH Antagonist Protocol Decrease the Live Birth Rate After Fresh Embryo Transfers but Have No Impact in Freeze-All Cycles.

Front Endocrinol (Lausanne) 2021 23;12:640047. Epub 2021 Apr 23.

Medical Center for Human Reproduction, Beijing Chao-Yang Hospital, Capital Medical University, Beijing, China.

Objective: To explore the association between serum LH levels and the cumulative live birth rate (CLBR) within one complete cycle, and the impact of serum LH levels on the live birth rate (LBR) after the initial embryo transfer (ET) considering different ET strategies (fresh or freeze-all).

Design: A retrospective cohort study.

Setting: University-affiliated reproductive center.

Patients: 1480 normogonadotrophic women who underwent COS with GnRH antagonist protocol for the first IVF/ICSI attempt.

Interventions: The sample was stratified into low and higher LH groups according to serum LH peak levels of <4 (Group A) and ≥4 IU/L (Group B) during COS. Patients were also sub-grouped into conventional fresh/frozen ET cycles and freeze-all cycles.

Main Outcome Measures: The LBR after the initial embryo transfer and the CLBR within one complete cycle.

Secondary Outcome Measures: The numbers of day-3 high-quality embryos, the numbers of embryos available, and the other pregnancy outcomes after the initial ET.

Results: In the whole cohort, the CLBRs decreased significantly in the low (63.1% vs. 68.3%, =.034) LH group compared to the higher LH group. Subgroup analysis revealed that patients with low LH levels had lower LBR after fresh ET (38.0% vs. 51.5%, =.005) but comparable LBR after the first frozen-thawed ET (FET) in freeze-all cycles (49.8% vs. 51.8%, =.517) than patients with higher LH peak levels. Likewise, patients with low LH levels had lower CLBR for conventional fresh/frozen ET cycles (54.8% vs. 66.1%, =.015) but comparable CLBR for the freeze-all cycles (66.8% vs. 69.2%, =.414) than those with higher LH levels. Following confounder adjustment, multivariable regression analyses showed that low LH level was an independent risk factor for the CLBR in the whole cohort (odds ratio (OR): 0.756, 95% confidence interval (CI): 0.604-0.965, =.014) and in patients who underwent the conventional ET strategy (OR: 0.596, 95% CI: 0.408-0.917, =.017). Moreover, the adverse impact of low LH levels on LBRs maintained statistically significant after fresh transfers (OR: 0.532, 95% CI: 0.353-0.800, =.002) but not after the first FETs in freeze-all cycles (OR: 0.918, 95% CI: 0.711-1.183,=.508).

Conclusions: In comparison with higher LH levels, low LH levels decrease the CLBRs per oocyte retrieval cycle for normogonadotrophic women who underwent COS using GnRH antagonists. This discrepancy may arise due to the significant detrimental effect of low LH levels on the LBRs after fresh embryo transfers.
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http://dx.doi.org/10.3389/fendo.2021.640047DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8104121PMC
April 2021

H. pylori is related to NAFLD but only in female: A Cross-sectional Study.

Int J Med Sci 2021 2;18(11):2303-2311. Epub 2021 Apr 2.

Beijing Shijitan Hospital, Capital Medical University, Beijing, China.

Recently, an increasing number of studies have focused on the extragastrointestinal effects of Helicobacter pylori (H. pylori), including metabolic syndrome, fatty liver, and rheumatic and skin diseases. Nonalcoholic fatty liver disease (NAFLD) is a common chronic disease worldwide that conveys a heavy economic burden on patients and society. The aim of this study was to investigate the relationship between H. pylori and NAFLD and to identify potential influencing factors. We conducted a cross-sectional study of individuals who had undergone regular physical examinations at the Beijing Shijitan Hospital Health Examination Center from July to October 2018. We evaluated the associations between NAFLD and NAFLD with H. pylori infection and related serum markers using multiple linear regression and logistic regression. There were significant relationships between H. pylori infection status and NAFLD in females (=0.034) but not in males (=0.795) according to Fisher's exact test. The association persisted after further adjustment for metabolic variables, gastrin factors, and liver enzymes. Waist-to-Hip Ratio, Body Mass Index, triglycerides, High-density lipoprotein cholesterol, glucose, uric acid, alkaline phosphatase, and Alanine aminotransferase are related to NAFLD after adjusting for age or interaction between biochemical indexes. H. pylori infection is related to NAFLD in female patients. The relationship between H. pylori and NAFLD may be mediated by markers of lipid metabolism and glycometabolism.
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http://dx.doi.org/10.7150/ijms.50748DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8100637PMC
April 2021

microRNA-210 and microRNA-3570 Negatively Regulate NF-κB-Mediated Inflammatory Responses by Targeting RIPK2 in Teleost Fish.

Front Immunol 2021 31;12:617753. Epub 2021 Mar 31.

Laboratory of Fish Molecular Immunology, College of Fisheries and Life Science, Shanghai Ocean University, Shanghai, China.

Pathogen infection can cause the production of inflammatory cytokines, which are key mediators that cause the host's innate immune response. Therefore, proper regulation of immune genes associated with inflammation is essential for immune response. Among them, microRNAs (miRNAs) as gene regulator have been widely reported to be involved in the innate immune response of mammals. However, the regulatory network in which miRNAs are involved in the development of inflammation is largely unknown in lower vertebrates. Here, we identified two miRNAs from miiuy croaker (), miR-210 and miR-3570, which play a negative regulatory role in host antibacterial immunity. We found that the expressions of miR-210 and miR-3570 were significantly upregulated under the stimulation of Gram-negative bacterium and LPS (lipopolysaccharide). Induced miR-210 and miR-3570 inhibit inflammatory cytokine production by targeting RIPK2, thereby avoiding excessive inflammation. In particular, we found that miR-210 and miR-3570 negatively regulate antimicrobial immunity by regulating the RIPK2-mediated NF-κB signaling pathway. The collective results indicated that both miRNAs are used as negative feedback regulators to regulate RIPK2-mediated NF-κB signaling pathway and thus play a regulatory role in bacteria-induced inflammatory response.
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http://dx.doi.org/10.3389/fimmu.2021.617753DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8044448PMC
June 2021

A Research on the Macroscopic and Mesoscopic Parameters of Concrete Based on an Experimental Design Method.

Materials (Basel) 2021 Mar 26;14(7). Epub 2021 Mar 26.

School of Water Conservancy and Hydroelectric Power, Hebei University of Engineering, Handan 056038, Hebei, China.

Concrete is a composite material that has complex mechanical properties. The mechanical properties of each of its components are different at the mesoscopic scale. Studying the relationship between the macroscopic and mesoscopic parameters of concrete can help better understand its mechanical properties at these levels. When using the discrete element method to model the macro-mesoscopic parameters of concrete, their calibration is the first challenge. This paper proposes a numerical model of concrete using the particle discrete element software particle flow code (PFC). The mesoscopic parameters required by the model need to be set within a certain range for an orthogonal experimental design. We used the proposed model to perform numerical simulations as well as response surface design and analysis. This involved fitting a set of mapping relationships between the macro-micro parameters of concrete. An optimization model was established in the MATLAB environment. The program used to calibrate the mesoscopic parameters of concrete was written using the genetic algorithm, and its macro-micro parameters were inverted. The following three conclusions can be drawn from the orthogonal test: First, the tensile strength and shear strength of the parallel bond between the particles of mortar had a significant influence on the peak compressive strength of concrete, whereas the influence of the other parameters was not significant. Second, the elastic modulus of the parallel bonding between particles of mortar, their stiffness ratio and friction coefficient, and the elastic modulus and stiffness ratio of contact bonding in the interfacial transition zone had a significant influence on the elastic modulus, whereas the influence of the other parameters was not significant. Third, the elastic modulus, stiffness ratio, and friction coefficient of the particles of mortar as well as the ratio of the contact adhesive stiffness in their interfacial transition zone had a significant influence on Poisson's ratio, whereas the influence of the other parameters was not significant. The fitting effect of the response surface design was good.
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http://dx.doi.org/10.3390/ma14071627DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8036469PMC
March 2021

Combined immunotherapy and targeted treatment for primary alveolar soft part sarcoma of the lung: case report and literature review.

Invest New Drugs 2021 Mar 25. Epub 2021 Mar 25.

Department of Oncology, Liaocheng People's Hospital, Liaocheng, 252000, Shandong Province, China.

Primary acinar soft part sarcoma of the lung (ASPS) is a rare malignancy with unique cellular structure and clinical and genetic characteristics. Most patients do not exhibit clear clinical symptoms, with only a few developing respiratory symptoms. The typical histological characteristics are acinoid or organ-like structures. Immunofluorescence in situ hybridization suggests a rearrangement of the transcription factor E3 gene. Patients respond poorly to chemotherapy and are, thus, primarily treated with surgery and targeted therapy. We report herein a unique case of primary alveolar soft part sarcoma of the lung. The patient was a 24-year-old man with metastases to multiple organs, such as the brain, lungs, pancreas, and liver. The craniocerebral lesions attained partial remission after whole-brain radiotherapy and targeted combined immunotherapy, and other distant metastases completely disappeared after targeted combined immunotherapy (anlotinib and camrelizumab), indicating significant treatment efficacy. Anlotinib is an oral multi-target tyrosine kinase inhibitor (TKI) that exerts its anti-tumor effects by acting on various kinases. Camrelizumab is a humanized immunoglobulin G4 monoclonal antibody that can target PD-1 to block the interaction between PD-L1 and programmed death ligand 2, ultimately causing an anti-tumor effect. This is the first report of successful use of anlotinib combined with camrelizumab in the treatment of advanced primary ASPS. The treatment benefit provides preliminary evidence that targeted therapy, combined with immunotherapy, may be a safe and effective approach to treat primary pulmonary ASPS patients, thus warranting further investigation.
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http://dx.doi.org/10.1007/s10637-021-01105-6DOI Listing
March 2021

Highly Selective Separation Intermediate-Size Anionic Pollutants from Smaller and Larger Analogs via Thermodynamically and Kinetically Cooperative-Controlled Crystallization.

Adv Sci (Weinh) 2021 Mar 1;8(6):2003243. Epub 2021 Feb 1.

School of Materials Science & Engineering Beijing Institute of Technology Beijing 100081 P. R. China.

Selective separation of organic species, particularly that of intermediate-size ones from their analogs, remains challenging because of their similar structures and properties. Here, a novel strategy is presented, cooperatively (thermodynamically and kinetically) controlled crystallization for the highly selective separation of intermediate-size anionic pollutants from their analogs in water through one-pot construction of cationic metal-organic frameworks (CMOFs) with higher stabilities and faster crystallization, which are based on the target anions as charge-balancing anions. 4,4'-azo-triazole and Cu are chosen as suitable ligand and metal ion for CMOF construction because they can form stronger intermolecular interaction with -toluenesulfonate anion (Ts-) compared to its analogs. For this combination, a condition is established, under which the crystallization rate of a Ts--based CMOF is remarkably high while those of analog-based CMOFs are almost zero. As a result, the faster crystallization and higher stability cooperatively endow the cationic framework with a close-to-100% selectivity for Ts- over its analogs in two-component mixtures, and this preference is retained in a practical mixture containing more than seven competing (analogs and inorganic) anions. The nature of the free Ts- anion in the cationic framework also allows the resultant CMOF to be recyclable via anion exchange.
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http://dx.doi.org/10.1002/advs.202003243DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7967070PMC
March 2021

Effects of intradialytic resistance exercises on physical performance, nutrient intake and quality of life among haemodialysis people: A systematic review and meta-analysis.

Nurs Open 2021 03 30;8(2):529-538. Epub 2019 Sep 30.

Institute of Longhua Clinical Medicine, Shanghai University of Traditional Chinese Medicine, Shanghai, China.

Objectives: The aim of this systematic reviews was to synthesize the current studies for the effectiveness of intradialytic resistance exercises with usual care on HD people.

Design: Meta-analysis of randomized controlled studies.

Methods: A systematic search of seven electronic databases, including PubMed, EMBASE, the Cochrane Library, Web of Science, WanFang, China National Knowledge Infrastructure (CNKI) and SINOMED, was systematically searched up to May 2018. The reference lists of previously reported systematic review were also checked. Pooled analysis was used to determine effection of intradialytic resistance exercises for haemodialysis people. Physical performance, nutrient intake and quality of life were explored, by comparing the association between effect sizes.

Results: Fourteen studies of 594 people were included. Compared with control groups, intradialytic resistance exercises significantly improve physical performance included 6-min walk test, sit-to-stand 30 and grip strength. However, no significant improvements were found in nutrient intakes such as dietary protein intake and quality of life.
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http://dx.doi.org/10.1002/nop2.274DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7877133PMC
March 2021

Long-circulating Doxorubicin and Schizandrin A Liposome with Drug-resistant Liver Cancer Activity: Preparation, Characterization, and Pharmacokinetic.

J Liposome Res 2021 Feb 8:1-50. Epub 2021 Feb 8.

College of Pharmaceutical, Heilongjiang University of Chinese Medicine, Harbin, Heilongjiang, China.

The selectivity of chemotherapeutic agents for liver cancer is poor. When they kill tumor cells, they produce serious adverse reactions in the whole body and multidrug resistance (MDR) is also a major hurdle in liver cancer chemotherapy. Combination therapy is a useful method for overcoming MDR and reducing toxic and side effects. In this study, we developed a long-circulating codelivery system, in which Doxorubicin (DOX) and Schizandrin A (SchA) are combined against MCF-7/ADR cells. The DOX-SchA long-circulating liposome (DOX-SchA-Lip) were prepared using ammonium sulfate gradient method. The two drugs were co-encapsulated into the Distearoyl phosphatidylethanolamine -polyethylene glycol(DSPE-mPEG2000) liposome and the liposome had an average particle size of (100 ± 3.5) nm and zeta electrical potential of (-31.3 ± 0.5) mV. The average encapsulation rate of DOX was 97.98% and that of SchA was 86.94%. DOX in liposome had good sustained-release effect. The results showed that DOX-SchA-Lip could significantly prolong the half-life (T) of the DOX and SchA, increase their circulation time in vivo, improve its bioavailability and reduce their side effects. Liposome can effectively induce early apoptosis of HepG2/ADR cells and the cell cycle was blocked in S-phase by DOX-SchA-Lip in a dose-dependent manner. The IC of compound liposome to HepG2 and HepG2/ADR were 0.55 μmol/L and 1.38 μmol/L respectively, which could significantly reverse the resistance of HepG2/ADR and the reversion multiple was 30.28. It was verified that DOX-SchA-Lip can effectively kill tumor cells and reverse MDR.
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http://dx.doi.org/10.1080/08982104.2021.1884093DOI Listing
February 2021

Circular RNA circPIKfyve acts as a sponge of miR-21-3p to enhance antiviral immunity through regulating MAVS in teleost fish.

J Virol 2021 Feb 3. Epub 2021 Feb 3.

Laboratory of Fish Molecular Immunology, College of Fisheries and Life Science, Shanghai Ocean University, Shanghai, China

Circular RNAs (circRNAs) are a class of widespread and diverse covalently closed circular endogenous RNAs that exert crucial functions in regulating gene expression in mammals. However, the function and regulation mechanism of circRNAs in lower vertebrates are still unknown. Here, we discovered a novel circRNA derived from PIKfyve, named circPIKfyve, that is related to the antiviral responses in teleost fish. The results showed that circPIKfyve plays essential roles in host antiviral immunity and inhibition of SCRV replication. Moreover, we also found that the antiviral effect inhibited by miR-21-3p could be reversed with the addition of circPIKfyve. In mechanism, our data revealed that circPIKfyve is a competitive endogenous RNA (ceRNA) of MAVS by sponging miR-21-3p, leading to activation of NF-κB/IRF3 pathway, which then enhance the innate antiviral responses. In addition, we firstly found that RNA binding protein QKI is involved in the formation and regulation of circPIKfyve. Our results provided a strong basis that circRNAs to play a regulatory role in antiviral immune responses in teleost fish. Here, we identified a novel circRNA, namely, circPIKfyve, that can act as a key regulator of the innate immune response in teleost fish. circPIKfyve acts as a molecular sponge by competitive adsorbing of miR-21-3p, thereby increasing the abundance of MAVS and activating the downstream NF-κB/IRF3 pathway to enhance the antiviral response. In addition, this study was the first to find that QKI protein is involved in regulating the formation of circPIKfyve in fish. The overall results of this study suggest that circPIKfyve plays an active regulatory role in the antiviral immune response of teleost fish.
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http://dx.doi.org/10.1128/JVI.02296-20DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8103680PMC
February 2021

Improve the performance of independent component analysis by mapping the spectrum to an orthogonal space.

Spectrochim Acta A Mol Biomol Spectrosc 2021 Apr 19;251:119467. Epub 2021 Jan 19.

School of Chemical Engineering, The University of Queensland, St Lucia, QLD 4072, Australia. Electronic address:

Independent Component Analysis (ICA) has attracted chemists recently, for its charm can separate the independent signals from a mixed system and does not need prior knowledge. However, its dissatisfactory performance for the chemical measured signal is still blocking the practicability. Thus, this paper summarized the ICA processing path from the establishment of rectangular coordinates in linear space to the determination of the corresponding relation between the coordinate system and real components. The primary cause of the deviation between the ICA results and the chemical measurements is that the measuring signal was subject to uncertainty. Besides, uncertainty made the deviation of source signal from the statistical independence assumption, or in other words, it appeared to be nonorthogonal. For this key, it proposed to map the measured value to the high-order derivative space, use the derivative to narrow the peak width, reduce the influence of uncertainty, and improve the separation performance of ICA to chemical measurement signal, such as the spectrum. Actual cases of this paper showed that when up to 6th order, the separating results had been perfect for IR spectra, and even for homologs isomers.
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http://dx.doi.org/10.1016/j.saa.2021.119467DOI Listing
April 2021

Progress on the GntR family transcription regulators in bacteria.

Yi Chuan 2021 Jan;43(1):66-73

College of Life Science and Technology, Guangxi University, Nanning 530004, China.

In bacteria, GntR family transcription regulators are the widespread family of transcription factors. Members of this family consist of two functional domains, a conserved N-terminal DNA-binding domain that contains a typical helix-turn-helix (HTH) motif and a C-terminal effector-binding or oligomerization domain. Usually, the amino acid sequences of N-terminal DNA-binding domains are highly conserved, but differ in the C-terminal effector-binding or oligomerization domains. In the past several decades, many GntR family transcription regulators have been characterized in a number of bacteria. These regulators control a variety of cellular processes such as cell motility, glucose metabolism, bacterial resistance, pathogenesis and virulence. In this review, we summarized the discovery, C-terminal domains, biological function and regulation mode of GntR family transcription regulators. This review will help researchers to obtain more knowledge about the functions and mechanisms of the GntR family transcriptional regulatory factors.
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http://dx.doi.org/10.16288/j.yczz.20-245DOI Listing
January 2021

Subarachnoid hemorrhage caused by spontaneous intracranial hypotension: two rare cases report.

Int J Neurosci 2021 Feb 8:1-5. Epub 2021 Feb 8.

Department of Neurology, The First Medical Centre, Chinese PLA General Hospital, Beijing, China.

Background: Spontaneous intracranial hypotension (SIH) combined with subarachnoid hemorrhage (SAH) has rarely been reported. Herein, we report two patients with SIH who suffered from diffuse non-aneurysmal SAH and expanded the symptom spectrum of SIH.

Case Presentation: (1) A 55-year-old male was diagnosed with SIH based on orthostatic headache and diffuse pachymeningeal enhancement on brain MRI. One more month later, his headache was exacerbated, and brain CT showed diffuse SAH. Lumber puncture showed bloody cerebrospinal fluid (CSF) with a low CSF pressure of 20 mmHO after a 30 mL intrathecal injection of saline. The patient was treated with a lumbar epidural blood patch and recovered. (2) A 41-year-old male presented with orthostatic headache and nuchal pain. The brain CT scan confirmed the diagnosis of SAH. Brain MRI revealed diffuse dural thickening and bilateral frontoparietal subdural fluid collection. Lumber puncture showed bloody CSF with low CSF pressure. Then, an epidural blood patch was performed with satisfactory results.

Conclusion: Dilation and rupture of intracranial venous structures might play significant roles in SIH combined with SAH. We should be alert to SIH patients who develop a new persistent severe headache without relief after lying down or a suddenly changed state of consciousness.
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http://dx.doi.org/10.1080/00207454.2021.1881094DOI Listing
February 2021

Comprehensive analysis of lncRNAs N-methyladenosine modification in colorectal cancer.

Aging (Albany NY) 2021 01 20;13(3):4182-4198. Epub 2021 Jan 20.

The Gastroenterology Tumor and Microenvironment Laboratory, Department of Gastroenterology, The First Affiliated Hospital of Chengdu Medical College, Chengdu Medical College, Chengdu 610041, Sichuan, PR China.

Background: Long non-coding RNAs (lncRNAs) and their N6-methyladenosine (M6A) modifications are involved in cancer occurrence and development.

Methods: lncRNA M6A modification in colorectal cancer (CRC) was comprehensively analyzed for the first time.

Results: M6A levels of lnRNAs in CRC tissues were higher than those in tumor-adjacent normal tissues. A total of 8,332 M6A peaks were detected in 6,690 lncRNAs in CRC tissues. Approximately 91% of the modified lncRNAs had unique M6A modification peaks. A total of 383 lncRNAs were differentially methylated in CRC, of which 48.24% had a length of 1-1,000 bp. Most of these were located on chromosomes 1, 2, 7, 11, 16 and 19; 42.3% were within a sense-overlapping exon. RNA sequencing identified 163 differentially expressed lncRNAs in CRC. GO and KEGG analyses revealed that genes near differentially-methylated or -expressed lncRNAs were associated with CRC occurrence and development. Methylation was positively correlated with lncRNA expression levels in CRC and tumor-adjacent normal tissues. More unmethylated than M6A methylated lncRNA molecules were detected. A competing endogenous RNA (ceRNA) and lncRNA-mRNA expression-regulation network revealed a regulatory relationship between lncRNAs, microRNAs (miRNAs), and mRNAs.

Conclusions: The findings may help improve our understanding of lncRNA function in colorectal cancer.
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http://dx.doi.org/10.18632/aging.202383DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7906145PMC
January 2021

Targeted deletion of HAI-1 increases prostasin proteolysis but decreases matriptase proteolysis in human keratinocytes.

Hum Cell 2021 May 24;34(3):771-784. Epub 2021 Jan 24.

Lombardi Comprehensive Cancer Center, Department of Oncology, Georgetown University, W412 Research Building 3970 Reservoir Road NW, Washington, DC, 20057, USA.

Epidermal differentiation and barrier function require well-controlled matriptase and prostasin proteolysis, in which the Kunitz-type serine protease inhibitor HAI-1 represents the primary enzymatic inhibitor for both proteases. HAI-1, however, also functions as a chaperone-like protein necessary for normal matriptase synthesis and intracellular trafficking. Furthermore, other protease inhibitors, such as antithrombin and HAI-2, can also inhibit matriptase and prostasin in solution or in keratinocytes. It remains unclear, therefore, whether aberrant increases in matriptase and prostasin enzymatic activity would be the consequence of targeted deletion of HAI-1 and so subsequently contribute to the epidermal defects observed in HAI-1 knockout mice. The impact of HAI-1 deficiency on matriptase and prostasin proteolysis was, here, investigated in HaCaT human keratinocytes. Our results show that HAI-1 deficiency causes an increase in prostasin proteolysis via increased protein expression and zymogen activation. It remains unclear, however, whether HAI-1 deficiency increases "net" prostasin enzymatic activity because all of the activated prostasin was detected in complexes with HAI-2, suggesting that prostasin enzymatic activity is still under tight control in HAI-1-deficient keratinocytes. Matriptase proteolysis is, however, unexpectedly suppressed by HAI-1 deficiency, as manifested by decreases in zymogen activation, shedding of active matriptase, and matriptase-dependent prostasin zymogen activation. This suppressed proteolysis results mainly from the reduced ability of HAI-1-deficient HaCaT cells to activate matriptase and the rapid inhibition of nascent active matriptase by HAI-2 and other yet-to-be-identified protease inhibitors. Our study provides novel insights with opposite impacts by HAI-1 deficiency on matriptase versus prostasin proteolysis in keratinocytes.
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http://dx.doi.org/10.1007/s13577-021-00488-1DOI Listing
May 2021

A Highly Conserved Circular RNA circRasGEF1B Enhances Antiviral Immunity by Regulating miR-21-3p/MITA Pathway in Lower Vertebrates.

J Virol 2021 Jan 13. Epub 2021 Jan 13.

Laboratory of Fish Molecular Immunology, College of Fisheries and Life Science, Shanghai Ocean University, Shanghai, China

Circular RNAs (circRNAs) represent a class of widespread, diverse, and covalently closed circRNAs that function as microRNA (miRNA) sponges and crucial regulators of gene expression in mammals. However, the regulation and function of circRNAs in lower vertebrates are still unknown. Here, we first discover a highly conserved circRNA termed circRasGEF1B, which displays a high conservation from mammals to fish and serves as key regulator in eliciting antiviral immunity in teleost fish. Results indicate that circRasGEF1B was highly expressed in -infected tissues and cells. Functionally, miR-21-3p could inhibit cellular antiviral responses significantly, whereas circRasGEF1B counteract the effects of miR-21-3p. In mechanism, the results demonstrate that circRasGEF1B acts as a competing endogenous RNA (ceRNA) of miR-21-3p to relieve the repressive effect of miR-21-3p on its target MITA, then enhance the innate antiviral responses. Our results not only provide a novel insight into the functions of circRNAs in lower vertebrates, but broaden our understanding of circRNAs in viral infection. (SCRV) is a typical fish RNA rhabdovirus, which is one of the most significant viral pathogens in teleost fish and can cause severe hemorrhagic septicemia in freshwater and marine fishes. Here, we discovered a highly conserved circRNAs called circRasGEF1B, which acts as a key regulator for innate antiviral responses upon SCRV infection. circRasGEF1B acts as an endogenous sponge of miR-21-3p that downregulates miR-21-3p expression levels. circRasGEF1B is able to bind to miR-21-3p directly and regulates MITA expression. To our knowledge, this report is the first to characterize circRNA-miRNA regulatory networks that exist in lower vertebrates.
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http://dx.doi.org/10.1128/JVI.02145-20DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8092700PMC
January 2021

Application of arterial spin labeling and susceptibility weighted imaging in the diagnosis of ischemic cerebrovascular diseases.

Int J Clin Exp Pathol 2020 1;13(12):3052-3059. Epub 2020 Dec 1.

Department of Medical Imaging, Shandong Qianfoshan Hospital, Cheeloo College of Medicine, Shandong University Jinan 250012, China.

Objective: To investigate the application value of arterial spin labeling (ASL) and susceptibility weighted imaging (SWI) in the diagnosis of acute ischemic cerebrovascular disease (CVDs).

Methods: A total of 124 patients who received fluid attenuated inversion recovery (FLAIR), diffusion weighted imaging (DWI), ASL, time of flight magnetic resonance angiography (TOF-MRA) and SWI scan sequentially were included in this study. The area of the abnormal perfusion region was compared with that of the restricted diffusion region. The cerebral blood flow (CBF) value and apparent diffusion coefficient (ADC) value were compared in ischemic penumbra (IP), infarct core and mirror region. The susceptibility vessel sign (SVS) detection rate was compared with the major vessel severe stenosis or occlusion rate as revealed by MRA. A receiver operating characteristic curve (ROC) was used to analyze the value of SVS as revealed by SWI.

Results: In total, 124 cases were included in this study, and 77 cases showed acute cerebral infarction. Among the 77 cases, 59 cases showed an IP. There were significant differences in ADC and CBF values between the infarct core and mirror region (P < 0.01). There was no significant difference in ADC value between IP and mirror region (P = 0.176), but there was significant difference in CBF value between IP and mirror region (P < 0.01). There was no significant difference in SVS detection rate compared with the vessel severe stenosis or occlusion rate in MRA (P = 0.111). Based on the MRA standards, the area under curve (AUC) of ROC for the SVS as revealed by SWI was 0.86 (95% CI: 0.753-0.962).

Conclusions: ASL combined with DWI contributed to IP evaluation of acute cerebral infarction. SWI showed higher diagnostic value for intravascular thrombus in acute cerebral infarction.
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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7791372PMC
December 2020

Synthesis and characterization of porous CaCO microspheres templated by yeast cells and the application as pH value-sensitive anticancer drug carrier.

Colloids Surf B Biointerfaces 2021 Mar 26;199:111545. Epub 2020 Dec 26.

Department of Biomedical Engineering, Research Center for Nano-Biomaterials & Regenerative Medicine, College of Biomedical Engineering, Taiyuan University of Technology, Taiyuan, Shanxi, 030024, China.

Using yeast as organic template and PDDA/PSS with opposite charge as polyelectrolyte, CaCO was deposited on yeast cells by Layer-by-Layer self-assembly method, and then porous calcium carbonate hybrid microspheres (CaCO-HMPs) were prepared by calcination. The CaCO-HMPs were characterized by FT-IR, XRD, SEM and TG. It was found that the prepared CaCO-HMPs were nearly spherical, with visible pores on the surface, small particle size uniformity (d = 3 μ m) and good dispersion. Doxorubicin hydrochloride (DOX) was used as the model drug to study drug loading and release properties of CaCO-HMPs. Then, the drug loading, DOX release under different pH conditions, and the degradation of CaCO-HMPs under different pH conditions were investigated. The drug release test results showed that the DOX-loaded microspheres released more drugs (99 %) at pH = 4.8 than pH = 7. It indicated that the CaCO-HMPs were pH sensitive. The cytotoxicity of DOX-loaded microspheres was also studied. It was found that CaCO-HMPs had good biocompatibility. In addition, compared with DOX group, cytotoxicity test results showed that the DOX-loaded microspheres had the same efficacy but sustained drug release for up to 120 h. Therefore, the CaCO-HMP microspheres have good application prospects as anticancer drug carriers.
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http://dx.doi.org/10.1016/j.colsurfb.2020.111545DOI Listing
March 2021

Unveiling the Electrooxidation of Urea: Intramolecular Coupling of the N-N Bond.

Angew Chem Int Ed Engl 2021 03 22;60(13):7297-7307. Epub 2021 Feb 22.

State Key Laboratory of Chem/Bio-Sensing and Chemometrics, College of Chemistry and Chemical Engineering, Hunan University, Changsha, Hunan, 410082, P. R. China.

The nitrogenous nucleophile electrooxidation reaction (NOR) plays a vital role in the degradation and transformation of available nitrogen. Focusing on the NOR mediated by the β-Ni(OH) electrode, we decipher the transformation mechanism of the nitrogenous nucleophile. For the two-step NOR, proton-coupled electron transfer (PCET) is the bridge between electrocatalytic dehydrogenation from β-Ni(OH) to β-Ni(OH)O, and the spontaneous nucleophile dehydrogenative oxidation reaction. This theory can give a good explanation for hydrazine and primary amine oxidation reactions, but is insufficient for the urea oxidation reaction (UOR). Through operando tracing of bond rupture and formation processes during the UOR, as well as theoretical calculations, we propose a possible UOR mechanism whereby intramolecular coupling of the N-N bond, accompanied by PCET, hydration and rearrangement processes, results in high performance and ca. 100 % N selectivity. These discoveries clarify the evolution of nitrogenous molecules during the NOR, and they elucidate fundamental aspects of electrocatalysis involving nitrogen-containing species.
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http://dx.doi.org/10.1002/anie.202015773DOI Listing
March 2021

Scutellarin Reduce the Homocysteine Level and Alleviate Liver Injury in Type 2 Diabetes Model.

Front Pharmacol 2020 11;11:538407. Epub 2020 Dec 11.

Department of Geriatrics, Gan Mei Hospital, The First People Hospital of Kunming City, Kunming, China.

Scutellarin (SCU) is an active ingredient extracted from . Its main physiological functions are anti-inflammatory and antioxidant. In this study, we established a STZ-induced model of type 2 diabetes (T2DM) and a homocysteine (Hcy)-induced apoptosis model of LO2 to investigate whether SCU can alleviate liver damage by regulating Hcy in type 2 diabetes. Biochemical analysis indicated that SCU could improve the lipid metabolism disorder and liver function in diabetic rats by downregulating the levels of triglycerides (TG), cholesterol (CHO), low-density lipoprotein (LDL), alanine transaminase (ALT) and aspartate transaminase (AST), and by upregulating the level of high-density lipoprotein (HDL). Interestingly, SCU also could down-regulate the levels of Hcy and insulin and enhance the ability of type 2 diabetic rats to regulate blood glucose. Mechanistically, our results indicated that SCU may control the level of Hcy through regulating the levels of β-Cystathionase (CBS), γ-Cystathionase (CSE) and 5,10-methylenetetrahydrofolate (MTHFR) in liver tissue, and up-regulate folic acid, VitB and VitB levels in serum. Furthermore, SCU inhibits apoptosis in the liver of T2DM rats and in cultured LO2 cells treated with Hcy. Together, our findings suggest that SCU may alleviate the liver injury thorough downregulating the level of Hcy in T2DM rats.
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http://dx.doi.org/10.3389/fphar.2020.538407DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7759645PMC
December 2020

Designed electron-deficient gold nanoparticles for a room-temperature C-C coupling reaction.

Chem Commun (Camb) 2021 Jan;57(6):741-744

School of Chemistry and Chemical Engineering, Shanghai Jiao Tong University, Shanghai 200240, P. R. China.

Stille cross-coupling reactions catalysed by an ideal catalyst combining the high activity of homogeneous catalysts and the reusability of heterogeneous catalysts are of great interest for C-C bond formation, which is a widely used reaction in fine chemistry. Despite great effort to increase the utilization ratio of surface metal atoms, the activity of heterogeneous catalysts under mild conditions remains unsatisfactory. Herein, we design a proof-of-concept strategy to trigger the room-temperature activity of heterogeneous Au catalysts by decreasing the electron density at the interface of a rationally designed Schottky heterojunction of Au metals and boron-doped carbons. The electron-deficient Au nanoparticles formed as a result of the rectifying contact with boron-doped carbons facilitate the autocleavage of C-Br bonds for highly efficient C-C coupling reactions of alkylbromides and allylstannanes with a TOF value of 5199 h-1 at room temperature, surpassing that of the state-of-the-art homogeneous catalyst.
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http://dx.doi.org/10.1039/d0cc06764dDOI Listing
January 2021

Diagnostic value of novel retroflexion colonoscopy in the right colon: A randomized controlled trial.

World J Gastrointest Oncol 2020 Nov;12(11):1336-1345

Department of Gastroenterology, Beijing Shijitan Hospital, Capital Medical University, Beijing 100038, China.

Background: Colonoscopy is the accepted gold standard for the detection of colorectal cancer. However, colonoscopy is less effective in preventing colon cancer in the right side compared with the left side.

Aim: To investigate the feasibility of a novel type of retroflexion colonoscope, EC-3490Ti colonoscope, for detection of proximal colon lesions.

Methods: In this prospective trial, we recruited patients who underwent colonoscopy for screening or surveillance. When the endoscopists could not grasp the whole observation of the right-side colon mucosa in the forward view (FV), insertion and withdrawal were repeatedly performed in the FV group with the EC38-i10F colonoscope while retroflexion was performed in the retroflexed view (RV) group with the EC-3490Ti colonoscope. Adenoma detection rate, the total number of adenomas per positive participant, the success rate of retroflexion, and endoscope withdrawal time were recorded and compared.

Results: The total adenoma detection rate (39.3% 37.7%, = 0.646) did not show any significant difference between the two groups. However, the polyp detection rate (59.6% 51.0%, = 0.002), adenoma detection rate in the right colon (21.6% 14.4%, = 0.012), and the total number of adenomas per positive participant (2.1 1.7, = 0.011) reached statistical significance. Retroflexion was achieved in 91.7% of our cohort. Compared with the FV group, the withdrawal time was significantly prolonged in the RV group (586.1 ± 124.4 s 508.8 ± 129.6 s, < 0.001). In contrast, the proportion of additional ancillary pressure decreased (27.4% 45.7%, < 0.001), and the visual analog scale pain scores did not increase (2.7 ± 1.4 2.8 ± 1.4, = 0.377).

Conclusion: Retroflexion in the proximal colon could be performed successfully and safely with the EC-3490Ti colonoscope. This maneuver could detect more adenomas effectively.
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http://dx.doi.org/10.4251/wjgo.v12.i11.1336DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7667455PMC
November 2020
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