Publications by authors named "Hui Song"

529 Publications

Sulforaphane alleviates LPS-induced inflammatory injury in ARPE-19 cells by repressing the PWRN2/NF-kB pathway.

Immunopharmacol Immunotoxicol 2022 Jun 29:1-9. Epub 2022 Jun 29.

Eye Center, The Central Hospital of Enshi Tujia and Miao Autonomous Prefecture, Enshi, P.R. China.

Background: Age-related macular degeneration (AMD) is the leading cause of blindness in the elderly population and its pathogenesis has been associated with inflammatory damage to retinal pigment epithelial (RPE) cells. Here, we explored the ability of sulforaphane to protect ARPE-19 cells from lipopolysaccharide (LPS)-induced inflammatory injury and elucidated the underlying molecular mechanism.

Methods: Cell viability, apoptosis, inflammation, PWRN2 expression, nuclear transcription factor-kappa B (NF-kB) activity, and the interaction between PWRN2 and the IkBa protein were assessed in RPE cells under- or over-expressing PWRN2 that had been treated with LPS and sulforaphane.

Results: Overexpression of PWRN2 in LPS-treated cells promoted NF-kB activation by interacting with IkBa, thus reducing cell viability. In contrast, PWRN2 downregulation repressed LPS-induced NF-kB activation and apoptosis in RPE cells. Similarly, sulforaphane downregulated PWRN2 and inhibited NF-kB activation in a concentration-dependent manner. Conversely, PWRN2 overexpression or NF-kB upregulation weakened the anti-inflammatory effects of sulforaphane.

Conclusion: Our results suggest that sulforaphane protects RPE cells from LPS-induced inflammatory injury by suppressing the PWRN2/NF-kB pathway.
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http://dx.doi.org/10.1080/08923973.2022.2090954DOI Listing
June 2022

De novo genes in Arachis hypogaea cv. Tifrunner: systematic identification, molecular evolution, and potential contributions to cultivated peanut.

Plant J 2022 Jun 24. Epub 2022 Jun 24.

College of Agronomy, Qingdao Agricultural University, Qingdao, China.

De novo genes are derived from non-coding sequences, and they can play essential roles in organisms. Cultivated peanut (Arachis hypogaea) is a major oil and protein crop derived from a cross between A. duranensis and A. ipaensis. However, few de novo genes have been documented in Arachis. Here, we identified 381 de novo genes in A. hypogaea cv. Tifrunner based on comparison with five closely related Arachis species. There are distinct differences in gene expression patterns and gene structures between conserved and de novo genes. The identified de novo genes originated from ancestral sequence regions associated with metabolic and biosynthetic processes, and they were subsequently integrated into existing regulatory networks. De novo paralogs and homoeologs were identified in A. hypogaea cv. Tifrunner. De novo paralogs and homoeologs with conserved expression have mismatching cis-acting elements under normal growth conditions. De novo genes potentially have pluripotent functions in responses to biotic stresses as well as in growth and development based on quantitative trait loci data. This work provides a foundation for future research examining gene birth process and gene function in Arachis and related taxa.
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http://dx.doi.org/10.1111/tpj.15875DOI Listing
June 2022

Enhanced Silk Fibroin/Sericin Composite Film: Preparation, Mechanical Properties and Mineralization Activity.

Polymers (Basel) 2022 Jun 17;14(12). Epub 2022 Jun 17.

College of Textile and Clothing Engineering, Soochow University, Suzhou 215123, China.

The periosteum plays an important role in bone formation and reconstruction. One of the reasons for the high failure rate of bone transplantation is the absence of the periosteum. Silk fibroin (SF) and silk sericin (SS) have excellent biocompatibility and physicochemical properties, which have amazing application prospects in bone tissue engineering, but lacked mechanical properties. We developed a series of SF/SS composite films with improved mechanical properties using boiling water degumming, which caused little damage to SF molecular chains to retain larger molecules. The Fourier transform infrared spectroscopy and X-ray diffraction results showed that there were more β-sheets in SF/SS films than in NaCO degummed SF film, resulting in significantly improved breaking strength and toughness of the composite films, which were increased by approximately 1.3 and 1.7 times, respectively. The mineralization results showed that the hydroxyapatite (HAp) deposition rate on SF/SS composite films was faster than that on SF film. The SF/SS composite films effectively regulated the nucleation, growth and aggregation of HAp-like minerals, and the presence of SS accelerated the early mineralization of SF-based materials. These composite films may be promising biomaterials in the repair and regeneration of periosteum.
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http://dx.doi.org/10.3390/polym14122466DOI Listing
June 2022

Accuracy of two orthodontic mini-implant templates in the infrazygomatic crest zone: a prospective cohort study.

BMC Oral Health 2022 Jun 24;22(1):252. Epub 2022 Jun 24.

Department of Stomatology, Beijing Friendship Hospital, Capital Medical University, No. 95 Yong'an Road, Xicheng District, Beijing, China.

Background: In the clinic, most computer-aided design and manufacturing orthodontic mini-implant guides are suitable for the position between the tooth roots, and few templates are designed and used for the infrazygomatic crest zone. In this study, we took into account the structure of the infrazygomatic crest and 3D printing technology, developed two kinds of templates, and evaluated their clinical effects.

Methods: Seventeen patients who accepted 30 mini-implant insertions in the infrazygomatic crest were selected. According to different implantation methods, three groups were divided. In Groups A and B, the mini-implants were positioned with an A-type or B-type template designed by EXOCAD software. In Group C, the mini-implants were inserted by an experienced orthodontist without any guides. We simulate the bucco-palatal, mesio-distal, and vertical head positions in the Segma implant guide software and measure the deviation from the virtual design position of the mini-implant. The linear deviation of the mini-implant tip and cap and the angular deviation of the long axis of the mini-implant in the bucco-palatal direction, mesio-distal direction, and vertical direction were also measured. The results were statistically analysed by SPSS software.

Results: The deviations of Group A and Group C's miniscrew cap in the bucco-palatal direction, Group A and Group B, Group A and Group C's miniscrew tip in the mesio-distal direction, and Group B and Group C's miniscrew tip and cap in the vertical direction were statistically significant (P < 0.05). There was a significant difference in the deviations of Group A and Group C's miniscrew tip and cap in the vertical direction (P < 0.01).

Conclusions: In the vertical direction, the accuracy of implantation with the template is higher than that of the traditional method without the template to avoid piercing the maxillary sinus mucosa in the infrazygomatic crest zone.
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http://dx.doi.org/10.1186/s12903-022-02285-0DOI Listing
June 2022

Genes Improve Drought Tolerance in .

Front Plant Sci 2022 26;13:910408. Epub 2022 May 26.

Grassland Agri-Husbandry Research Center, College of Grassland Science, Qingdao Agricultural University, Qingdao, China.

WRKY transcription factor participates in plant growth and development and response to biotic and abiotic stresses. , a turfgrass, has high drought tolerance, yet little is known about response to drought stress in . In this study, RNA-seq identified five , including , , , , and , which were upregulated under drought stress. Orthologous relationships between and were determined to predict the regulatory networks of the five AdWRKYs based on AtWRKYs. Additionally, protein-protein interactions were predicted using differentially expressed proteins from RNA-seq. The quantitative real-time PCR (qRT-PCR) results showed that was upregulated, while , , and were downregulated at different time-points under drought stress. The predicted regulatory networks showed that AdWRKY40 activates , , and expression under drought stress. Besides, AdWRKY56 regulated under drought stress. Aradu.YIQ80 (NAC019) interacted with AdWRKY40, AdWRKY42, AdWRKY56, and AdWRKY64, while Aradu.Z5H58 (NAC055) interacted with AdWRKY42 and AdWRKY64 under drought stress. This study used to assess function and regulatory networks, providing a basis for understanding drought tolerance in .
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http://dx.doi.org/10.3389/fpls.2022.910408DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9199494PMC
May 2022

Identification of safe channels for screws in the anterior pelvic ring fixation system.

J Orthop Surg Res 2022 Jun 11;17(1):312. Epub 2022 Jun 11.

The Third Affiliated Hospital, Southern Medical University, Guangzhou, Guangdong, People's Republic of China.

Background: Minimally invasive surgery for pelvic fracture using anterior ring internal fixator system is increasing gradually, and the way to insert the fixation screws in the fixation system is the key technical points of the method. However, there have been few studies on insertion of fixation screws for the anterior pelvic ring internal fixator system.

Objective: To identify safe channels for fixation screws in the anterior pelvic fixator system and provide the anatomical basis for insertion of fixation screws in clinical operation.

Methods: Screw insertion was simulated into a total of 40 pelvic finite element models as well as 16 fresh pelvic specimens, and the channel parameters were measured.

Results: Finite elements (male, female) include: screws in ilium: length 114.4 ± 4.1 and 107.6 ± 8.3 mm, respectively; diameter 11.7 ± 0.5 and 10.0 ± 0.6 mm, distance between screw and anterior inferior iliac spine: 5.5 ± 1.0 and 5.6 ± 1.0 mm, angle of coronal plane 55.8° ± 2.4° and 50.6° ± 3.1°, angle of sagittal plane 26.6° ± 1.0° and 24.5° ± 1.9° and angle of horizontal plane 64.9 ± 3.7 and 58.1 ± 3.1; screws in pubis: length 47.0 ± 2.0 and 39.8 ± 3.9 mm, diameter 7.1 ± 0.4 and 6.1 ± 0.4 mm. Specimens (male, female) include: distance between screw and anterior inferior iliac spine: 5.5 ± 0.5 and 5.6 ± 0.7 mm, angle of coronal plane 55.9° ± 1.3° and 50.7° ± 1.5°, angle of sagittal plane 26.7° ± 0.5° and 24.1° ± 0.9° and angle of horizontal plane 64.8° ± 0.6° and 58.8° ± 0.8°. In the comparison between female and male in each group, differences in distances between screws and anterior inferior iliac spine and median line of symphysis pubis (P > 0.05) were not statistically significant; differences in the remaining parameters were statistically significant (P < 0.05).

Conclusions: If surgeons paid attention to sex differences, select screws of appropriate diameter and length and hold the insertion position and direction, screws in the anterior pelvic ring fixation system could be safely inserted.
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http://dx.doi.org/10.1186/s13018-022-03191-5DOI Listing
June 2022

High-Throughput Sequencing to Investigate lncRNA-circRNA-miRNA-mRNA Networks Underlying the Effects of Beta-Amyloid Peptide and Senescence on Astrocytes.

Front Genet 2022 12;13:868856. Epub 2022 May 12.

Key Laboratory of Endemic and Ethnic Diseases, Ministry of Education and Key Laboratory of Medical Molecular Biology of Guizhou Province, Guizhou Medical University, Guiyang, China.

Astrocytes are widely distributed in the central nervous system and play an essential role in the function of neuronal cells. Associations between astrocytes and Alzheimer's disease (AD) have been noted, and recent work has implicated circular RNA (circRNA) and long non-coding RNA (lncRNA) in the development of AD. However, few reports have investigated which lncRNA and circRNA are involved in the influence of amyloid beta (Aβ) and senescence on astrocytes. This study therefore examines changes at the transcriptome level to explore the effects of Aβ and senescence on astrocytes. Primary cultured astrocytes were treated with Aβ and cultured for 90 days , and high-throughput sequencing was performed to identify differentially expressed RNAs. Gene Ontology and Kyoto Encyclopedia of Genes and Genomes enrichment analyses revealed that differentially expressed genes were associated with the focal adhesion signaling pathway, extracellular matrix receptor signaling pathway, and the extracellular matrix. The protein-protein interaction network was then constructed, and 103 hub genes were screened out; most of these were strongly associated with the expression of the extracellular matrix, extracellular matrix receptor signaling pathway, and focal adhesion. Two competing endogenous RNA networks were constructed based on the selected hub gene and differential RNAs, and we identified multiple competing endogenous RNA regulatory axes that were involved in the effects of Aβ and senescence on astrocytes. This is the first study to explore the molecular regulation mechanism of Aβ and senescence on primary astrocytes from the perspective of the whole transcriptome. In uncovering the signaling pathways and biological processes involved in the effects of Aβ and senescence on astrocytes, this work provides novel insights into the pathogenesis of AD at the level of competing endogenous RNA network regulation.
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http://dx.doi.org/10.3389/fgene.2022.868856DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9133720PMC
May 2022

Interaction between gut microbiota dysbiosis and lung infection as gut-lung axis caused by Streptococcus suis in mouse model.

Microbiol Res 2022 Aug 25;261:127047. Epub 2022 Apr 25.

College of Animal Science and Veterinary Medicine, Huazhong Agricultural University, Wuhan 430070, China. Electronic address:

Streptococcus suis (S. suis) is an important zoonotic pathogen threatening the global pig farming industry. It causes respiratory and digestive tract infections simultaneously in pigs. The balanced gut microbiota not only affects the local mucosal immune response but also involves the regulation of the immune status of the distant lung tissues that is termed as "gut-lung" axis. Whether S. suis affects the gut during lung infection and how does the intestinal microbial disturbance play role in the development of lung infection during S. suis exposure is not clear yet. Therefore, in the current study, we constructed the animal model using six-week-old mice (N = 48) divided into four groups with S. suis serotype 2 (SS2)-induced lung infection and the antibiotic treated gut microbiota dysbiosis. By means of various techniques (like HE staining, RT-qPCR, Western Blot and ELISA and viability detection) we explored that S. suis can concurrently cause intestinal tissue damage and inflammation after lung infection. Moreover, gut microbiota dysbiosis changes the balance of Th1/Th2 cells that aggravates lung injury during the infection. Thus, "gut-lung" axis of the communication between the gut microbiota and lung infection was established through the spleen and blood. In addition, intestinal dysbacteriosis can affect alveolar macrophage activity for a long time and the balance of gut microbiota plays an important role in lung infection caused by S. suis. Hence, this study clarified the pulmonary infection caused by SS2 from the perspective of the intestinal microbiota providing novel theoretical basis for the treatment of related lung diseases.
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http://dx.doi.org/10.1016/j.micres.2022.127047DOI Listing
August 2022

Clinical Efficacy of Acupuncture for the Treatment of Rheumatoid Arthritis: Meta-Analysis of Randomized Clinical Trials.

Evid Based Complement Alternat Med 2022 30;2022:5264977. Epub 2022 Apr 30.

Department of Rheumatology, Beijing Jishuitan Hospital, Beijing 100035, China.

Objective: Acupuncture has been used by rehabilitation specialists as an adjunctive treatment for the symptomatic treatment of rheumatoid arthritis (RA). This meta-analysis aims to evaluate the efficacy of acupuncture in treating patients with RA.

Methods: A comprehensive search was conducted in CBM, CNKI, PubMed, CENTRAL, Web of Science, and Embase from their inception up to March 2022. All randomized controlled trials (RCTs) without the language restriction, concerning the patients with RA treated with acupuncture, were included. Two reviewers independently assessed the risk of bias with the Cochrane Risk of Bias Assessment tool. Weight mean difference (MD) and 95% CI were calculated, and data were pooled with random effects model.

Results: A total of eleven RCTs involving 796 patients with RA met the established inclusion criteria. This systematic review indicated the efficacy of acupuncture as an adjunctive treatment for patients with RA. Invasive acupuncture could reduce significantly in pain (MD = -1.00, 95% CI: -1.96 to -0.05, =0.040), health assessment questionnaire (HAQ, MD = -0.20, 95% CI: -0.30 to -0.11, < 0.001), physician global assessment (PhGA, MD = -0.98, 95% CI: -1.23 to -0.72, < 0.001), tender joint count (TJC, MD = -1.24, 95% CI: -2.11 to -0.37, =0.005), C-reactive protein (CRP, MD = -1.81, 95% CI: -3.32 to -0.29, =0.019), and erythrocyte sedimentation rate (ESR, MD = -3.03, 95% CI: -5.80 to -0.26, =0.032), while compared to control group. Laser acupuncture could reduce HAQ (MD = -0.15, 95% CI: -0.28 to -0.01, =0.034), the RA quality of life questionnaire (RAQoL, MD = -2.32, 95% CI: -4.40 to -0.25, =0.028), CRP (MD = -35.24, 95% CI: -36.49 to -33.99, < 0.001), and interleukin-6 (IL-6, MD = -29.63, 95% CI: -49.34 to -9.92, =0.003), while compared to control group. No adverse events associated with acupuncture were reported.

Conclusion: Available evidence suggests that acupuncture is beneficial for relieving pain and ameliorating quality of life and health index in patients with RA; thereby, it should be available as an adjunctive nonpharmacological treatment in rehabilitation programmes.
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http://dx.doi.org/10.1155/2022/5264977DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9078778PMC
April 2022

Conjunctive Analyses of Bulk Segregant Analysis Sequencing and Bulk Segregant RNA Sequencing to Identify Candidate Genes Controlling Spikelet Sterility of Foxtail Millet.

Front Plant Sci 2022 14;13:842336. Epub 2022 Apr 14.

State Key Laboratory of Crop Stress Biology for Arid Areas, College of Agronomy, Northwest A&F University, Xianyang, China.

Foxtail millet has gradually become a model gramineous C crop owing to its short growth period and small genome. Research on the development of its spikelets is not only directly related to the yield and economic value of foxtail millet but also can provide a reference for studying the fertility of other C crops. In this study, a hybrid population containing 200 offspring was constructed from the Xinong8852 and An15 parental lines, and two extreme trait populations were constructed from the F2 generation for analysis of the spikelet sterility. The F2 population conformed to a 3:1 Mendelian segregation ratio, and it was thus concluded that this trait is likely controlled by a single recessive gene. Bulk segregant analysis sequencing (BSA-Seq) was used to determine the candidate regions and candidate genes related to the development of foxtail millet spikelets. Additionally, the functional analysis of differentially expressed genes in populations with different traits was conducted by bulk segregant RNA sequencing (BSR-Seq). Finally, conjunctive analysis of BSA-Seq and BSR-Seq results, combined with biological information analysis, revealed six genes on chromosome VII that were ultimately identified as candidate genes controlling foxtail millet spikelet development. This study provides a new reference for research on foxtail millet sterility and lays a solid foundation for the examination of fertility in other gramineous crops.
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http://dx.doi.org/10.3389/fpls.2022.842336DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9047506PMC
April 2022

Development and Validation of a Modeling and Calibration Method for Diesel-Like Multistage Combustion Based On a Modified Multi-Wiebe Function.

ACS Omega 2022 Apr 1;7(14):11756-11769. Epub 2022 Apr 1.

School of Energy and Power Engineering, Dalian University of Technology, Dalian, Liaoning 116024, China.

The stringent regulations of fuel consumption and exhaust emission require further refinement of the control strategy for diesel engines. In the future, the prediction of the in-cylinder combustion process will become necessary to achieve a more dedicated control performance. Hence, a more precise model able to run in a real-time application is required to predict the nature of multiphase Diesel combustion. This paper presents a modified multi-Wiebe function with a concise parameter structure, which is governed by the center point of the combustion process and the form factor of each stage. The modified function captures the typical characteristics of the measured heat release rate and avoids the ambiguous determination of several parameters, therefore improving the calibration efficiency. A novel calibration method called "backward-stepwise recursion" is introduced that decomposes the nature of the measured heat release rate and fits the function from the tail stage to the precombustion stage. This method is suitable for large-quantity diesel fuel combustion and dual-fuel combustion cases in which the adjacent combustion stages superimpose one another. The proposed method is applied in the measured heat release rate of a single-cylinder prototype diesel engine from 15% to 100% load conditions. The modified multi-Wiebe function suggests good accordance in heat release prediction at all the load conditions, which demonstrates its ability to be embedded in the control unit for crank-angle-resolved real-time combustion prediction.
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http://dx.doi.org/10.1021/acsomega.1c06858DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9016855PMC
April 2022

Platelets fine-tune effector responses of naïve CD4 T cells via platelet factor 4-regulated transforming growth factor β signaling.

Cell Mol Life Sci 2022 Apr 18;79(5):247. Epub 2022 Apr 18.

Department of Medicine-Solna, Cardiovascular Medicine Unit, J8:20, Karolinska Institute, Karolinska University Hospital-Solna, 171 76, Stockholm, Sweden.

Background And Aim: Platelets are an able regulator of CD4 T cell immunity. Herein, the mechanisms underlying platelet-regulated effector responses of naïve CD4 T (Tn) cells were investigated.

Methods: Platelet-Tn cell co-cultures of human cells, genetically modified murine models, and high-throughput bioinformatic analyses were combined to elucidate molecular mechanisms of platelet-dependent regulation.

Results: Platelets exerted sophisticated regulation on effector responses of type 1, 2, and 17 T helper (Th1/Th2/Th17) and regulatory T (Treg) cells, in time-, concentration-, and organ-dependent manners and with close cooperation of transforming growth factor β (TGFβ) and platelet factor 4 (PF4). PF4 at low concentrations reinforced TGFβ signaling by heteromerizing with type III TGFβ receptor (TGFBRIII), and subsequently enhanced TGFBRII expression and TGFβ signaling. High-concentration PF4 had, however, opposite effects by directly binding to TGFBRII, blocking TGFβ-TGFBRII ligation, and thus inhibiting TGFβ signaling. Furthermore, platelet depletion markedly hampered Treg and Th17 responses in the spleen but not in the lymph nodes, blockade of platelet-Tn cell contact diminished platelet effects, while spleen injection of PF4-immobilized microparticles in PF4-deficient mice mimicked platelet effects, suggesting the importance of direct platelet-Tn contact and platelet-bound PF4 for the optimal regulatory effects by platelets.

Conclusion: Platelets exert context-dependent regulations on effector responses of Tn cells via PF4-TGFβ duet, suggesting new possibilities of platelet-targeted interventions of T cell immunity.
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http://dx.doi.org/10.1007/s00018-022-04279-1DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9016031PMC
April 2022

High-quality chromosome-scale de novo assembly of the Paspalum notatum 'Flugge' genome.

BMC Genomics 2022 Apr 11;23(1):293. Epub 2022 Apr 11.

College of Grassland Science, Qingdao Agricultural University, Qingdao, 266109, China.

Background: Paspalum notatum 'Flugge' is a diploid with 20 chromosomes (2n = 20) multi-purpose subtropical herb native to South America and has a high ecological significance. It is currently widely planted in tropical and subtropical regions. Despite the gene pool of P. notatum 'Flugge' being unearthed to a large extent in the past decade, no details about the genomic information of relevant species in Paspalum have been reported. In this study, the complete genome information of P. notatum was established and annotated through sequencing and de novo assembly of its genome.

Results: The latest PacBio third-generation HiFi assembly and sequencing revealed that the genome size of P. notatum 'Flugge' is 541 M. The assembly result is the higher index among the genomes of the gramineous family published so far, with a contig N50 = 52Mbp, scaffold N50 = 49Mbp, and BUSCOs = 98.1%, accounting for 98.5% of the estimated genome. Genome annotation revealed 36,511 high-confidence gene models, thus providing an important resource for future molecular breeding and evolutionary research. A comparison of the genome annotation results of P. notatum 'Flugge' with other closely related species revealed that it had a close relationship with Zea mays but not close compared to Brachypodium distachyon, Setaria viridis, Oryza sativa, Puccinellia tenuiflora, Echinochloa crusgalli. An analysis of the expansion and contraction of gene families suggested that P. notatum 'Flugge' contains gene families associated with environmental resistance, increased reproductive ability, and molecular evolution, which explained its excellent agronomic traits.

Conclusion: This study is the first to report the high-quality chromosome-scale-based genome of P. notatum 'Flugge' assembled using the latest PacBio third-generation HiFi sequencing reads. The study provides an excellent genetic resource bank for gramineous crops and invaluable perspectives regarding the evolution of gramineous plants.
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http://dx.doi.org/10.1186/s12864-022-08489-6DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9004155PMC
April 2022

Development of candidate gene-based markers and map-based cloning of a dominant rust resistance gene in cultivated groundnut (Arachis hypogaea L.).

Gene 2022 Jun 4;827:146474. Epub 2022 Apr 4.

National Centre for Biological Sciences, Tata Institute of Fundamental Research, Bellary Road, Bangalore 560065, Karnataka, India. Electronic address:

A dominant rust resistance gene, VG 9514-Rgene was isolated through map-based cloning. Sequence analysis revealed non-synonymous mutations in the TIR, NBS and LRR region of the R-protein. Candidate gene-based markers from these SNPs revealed complete co-segregation of the isolated VG 9514-Rgene with rust resistance in a RIL population and confirmed their map position in between FRS 72 and SSR_GO340445 markers in arahy03 chromosome. Blastp search of VG 9514-Rprotein detected Arahy.T6DCA5 with >80.0% identity that localized at 142,544,745.0.142,549,184 in arahy03 chromosome. K/K calculation revealed that VG 9514-Rgene had undergone positive selection compared to four homologous genes in the groundnut genome. Homology based structure modelling of this R-protein revealed a typical consensus three-dimensional folding of TIR-NBS-LRR protein. Non-synonymous mutations in susceptible version of R-protein were mapped and found E268Q mutation in hhGRExE motif, Y309F in RNBS-A motif and I579T in MHD motif of NB-ARC domain are probable candidates for loss of function.
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http://dx.doi.org/10.1016/j.gene.2022.146474DOI Listing
June 2022

Five-year clinical outcomes of rigid iris-fixated phakic intraocular lens in northern Chinese.

Int Ophthalmol 2022 Apr 5. Epub 2022 Apr 5.

Tianjin Key Lab of Ophthalmology and Visual Science, Tianjin Eye Institute, Tianjin Eye Hospital, Nankai University Affiliated Eye Hospital, Clinical College of Ophthalmology Tianjin Medical University, No. 4 Gansu Road, Heping District, Tianjin, 300020, China.

Purpose: To investigate the 5-year clinic outcomes of rigid iris-fixated pIOL on the visual performance, complications and intraocular light scattering in northern Chinese.

Methods: Thirty eyes implanted with iris-fixated phakic IOLs (pIOLs group) and 34 eyes with high myopia (myopia group) were involved in this study. At preoperatively, 6 months, 1, 3, and 5 years postoperatively, the uncorrected visual acuity (UCVA), best-spectacle corrected visual acuity (BSCVA), endothelial cell density (ECD), and intraocular pressure (IOP) were measured in pIOLs group. The objective scatter index (OSI), modulation transfer function cut off (MTF cut off), and Strehl ratio were measured by Optical Quality Analysis System and a pseudophakic dysphotopsia questionnaire (PDQ) was used to evaluate the subjects' satisfaction in pIOLs and myopia groups.

Results: At 5 years postoperatively, an UCVA of 20/20 or better was found in 43.33% of eyes in pIOLs group. At 6 months, 1, 3, and 5 years postoperatively, the mean ECD decrease were 1.29% ± 0.45%, 2.59% ± 1.30%, 6.67% ± 2.26%, and 10.80% ± 3.48%. The value of OSI in pIOLs group was significantly higher than that in myopia group (P < 0.001). The PDQ results showed that the subjects in myopia group complained less with intolerance of bright lights than those in pIOLs group. The values of Strehl ratio and MTF cut off in pIOLs group were significantly lower than that in myopia group (P < 0.001).

Conclusions: Iris-fixated pIOL induce more intraocular light scattering. A significant decrease in ECD was observed at 5 years postoperatively. An annual evaluation of ECD is necessary for patients undergoing pIOL implantation.
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http://dx.doi.org/10.1007/s10792-022-02303-8DOI Listing
April 2022

Dissection of valine-glutamine genes and their responses to drought stress in Arachis hypogaea cv. Tifrunner.

Funct Integr Genomics 2022 Apr 2. Epub 2022 Apr 2.

Grassland Agri-husbandry Research Center, College of Grassland Science, Qingdao Agricultural University, Qingdao, China.

Valine-glutamine sequences (VQs) interact with WRKY transcription factors (TFs), forming VQ-WRKY protein complexes crucial for plant development and response to environmental changes. Cultivated peanut (Arachis hypogaea) is a tetraploid from A. duranensis and A. ipaensis cross. The Arachis spp. WRKY TFs have been identified, but Arachis VQs are largely unknown. This study identified VQs in A. duranensis, A. ipaensis, A. monticola, A. hypogaea cv. Fuhuasheng, A. hypogaea cv. Shitouqi, and A. hypogaea cv. Tifrunner. The study analyzed the homologous relationships between VQs in these Arachis spp. The VQ drought-tolerant genes were detected and VQ-WRKY interactions were determined in A. hypogaea cv. Tifrunner. The results showed that tetraploid Arachis spp. retained duplicated VQs, but lost ancestral VQs after allopolyploidization. The number of VQs in A. monticola, A. hypogaea cv. Fuhuasheng, and A. hypogaea cv. Shitouqi increased relative to their diploid ancestors. RNA-seq and quantitative real-time PCR experiments confirmed that three AhTVQs tolerate drought stress in A. hypogaea cv. Tifrunner. However, evidence of VQ-WRKY interaction for drought stress response is lacking in A. hypogaea cv. Tifrunner. Nevertheless, this study identified VQ-WRKY interactions, which possibly have multiple functions in A. hypogaea cv. Tifrunner. Altogether, this study dissected Arachis VQs, providing insights into Arachis VQ evolution and drought function.
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http://dx.doi.org/10.1007/s10142-022-00847-7DOI Listing
April 2022

An inactivating mutation in the vacuolar arginine exporter gene Vae results in culture degeneration in the fungus Metarhizium robertsii.

Environ Microbiol 2022 Mar 30. Epub 2022 Mar 30.

MOE Key Laboratory of Biosystems Homeostasis & Protection, Institute of Microbiology, College of Life Science, Zhejiang University, Hangzhou, China.

Culture degeneration usually results in great commercial losses in the economically important filamentous fungi, but the genetic causes of the degeneration remain elusive. In the fungus Metarhizium robertsii, we found that deletion of the vacuolar arginine exporter gene Vae caused culture degeneration. Compared to the WT strain, the mutant showed increased apoptosis, reactive oxygen species (ROS) level and mitochondrial membrane potential collapse, reduced conidial yield and abnormal lipid droplet formation. The extent of the degeneration in the mutant gradually increased over the successive subculturing, which eventually became irreversible; compared to the third subculture of the mutant, the seventh subculture showed a lower conidial yield and pathogenicity to insects, stronger apoptosis, higher ROS level and a smaller number of conidial lipid droplets. Incorporation of the genomic clone of Vae could not restore the WT phenotypes in the seventh subculture, but could in the third one. Loss-of-function in Vae resulted in vacuolar arginine accumulation and reduction in the cytosolic arginine. This downregulated the expression of the regulator CAG9 of G protein signalling pathway, which accounted for most of the phenotypic changes associated with the degeneration of the mutant. We identified a deleterious mutation that causes culture degeneration in a filamentous fugus.
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http://dx.doi.org/10.1111/1462-2920.15982DOI Listing
March 2022

MNB1 gene is involved in regulating the iron-deficiency stress response in Arabidopsis thaliana.

BMC Plant Biol 2022 Mar 28;22(1):151. Epub 2022 Mar 28.

School of Food and Biological Engineering, Hefei University of Technology, Hefei, 230009, China.

Background: Iron (Fe) is an essential mineral element that involves in many biological processes important for most plants growth and development. Fe-deficiency induces a complex series of responses in plants, involving physiological and developmental changes, to increase Fe uptake from soil. However, the molecular mechanism involved in plant Fe-deficiency is not well understood.

Results: Here, we found that the MNB1 (mannose-binding-lectin 1) gene is involved in the regulation of Fe-deficiency stress response in Arabidopsis thaliana. The expression abundance of MNB1 was inhibited by Fe-deficiency stress. Knockout of MNB1 led to enhanced Fe accumulation and tolerance, whereas the MNB1-overexpressing plants were sensitive to Fe-deficiency stress. Under conditions of normal and Fe-deficiency, lower HO concentrations were detected in mnb1 mutant plants compared to wild type. On the contrary, higher HO concentrations were found in MNB1-overexpressing plants, which was negatively correlated with malondialdehyde (MDA) levels. Furthermore, in mnb1 mutants, the transcription level of the Fe uptake- and translocation-related genes, FIT, IRT1, FRO2, ZIF, FRD3, NAS4, PYE and MYB72, were considerably elevated during Fe-deficiency stress, resulting in enhanced Fe uptake and translocation, thereby increasing Fe accumulation.

Conclusions: Together, our findings show that the MNB1 gene negatively controls the Fe-deficiency response in Arabidopsis via modulating reactive oxygen species (ROS) levels and the ROS-mediated signaling pathway, thereby affecting the expression of Fe uptake- and translocation-related genes.
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http://dx.doi.org/10.1186/s12870-022-03553-5DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8961904PMC
March 2022

Metabolic response of Lactobacillus acidophilus exposed to amoxicillin.

J Antibiot (Tokyo) 2022 May 24;75(5):268-281. Epub 2022 Mar 24.

Pharmaceutical College, Guangxi Medical University, Nanning, 530021, China.

Drug-induced diarrhea is a common adverse drug reaction, especially the one caused by the widespread use of antibiotics. The reduction of probiotics is one reason for intestinal disorders induced by an oral antibiotic. However, the intrinsic mechanism of drug-induced diarrhea is still unknown. In this study, we used metabolomics methods to explore the effects of the classic oral antibiotic, amoxicillin, on the growth and metabolism of Lactobacillus acidophilus, while scanning electron microscopy (SEM) and 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assays were employed to evaluate changes in cell activity and morphology. The results showed that cell viability gradually decreased, while the degree of cell wall rupture increased, with increasing amoxicillin concentrations. A non-targeted metabolomics analysis identified 13 potential biomarkers associated with 9 metabolic pathways. The data showed that arginine and proline metabolism, nicotinate and nicotinamide metabolism, pyrimidine metabolism, glycine, serine and threonine metabolism, beta-alanine metabolism, glycerolipid metabolism, tryptophan metabolism, steroid hormone biosynthesis, and histidine metabolism may be involved in the different effects exerted by amoxicillin on L. acidophilus. This study provides potential targets for screening probiotics regulators and lays a theoretical foundation for the elucidation of their mechanisms.
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http://dx.doi.org/10.1038/s41429-022-00518-6DOI Listing
May 2022

Promoter Hypomethylation of TGFBR3 as a Risk Factor of Alzheimer's Disease: An Integrated Epigenomic-Transcriptomic Analysis.

Front Cell Dev Biol 2021 2;9:825729. Epub 2022 Mar 2.

Key Laboratory of Endemic and Ethnic Diseases, Ministry of Education and Key Laboratory of Medical Molecular Biology of Guizhou Province, School of Basic Medical Sciences, Guizhou Medical University, Guiyang, China.

Alzheimer's disease (AD) is characterized by the abnormal deposition of amyloid-β (Aβ) plaques and tau tangles in the brain and accompanied with cognitive impairment. However, the fundamental cause of this disease remains elusive. To elucidate the molecular processes related to AD, we carried out an integrated analysis utilizing gene expression microarrays (GSE36980 and GSE5281) and DNA methylation microarray (GSE66351) in temporal cortex of AD patients from the Gene Expression Omnibus (GEO) database. We totally discovered 409 aberrantly methylated and differentially expressed genes. These dysregulated genes were significantly enriched in biological processes including cell part morphogenesis, chemical synaptic transmission and regulation of Aβ formation. Through convergent functional genomic (CFG) analysis, expression cross-validation and clinicopathological correlation analysis, higher TGFBR3 level was observed in AD and positively correlated with Aβ accumulation. Meanwhile, the promoter methylation level of TGFBR3 was reduced in AD and negatively associated with Aβ level and advanced Braak stage. Mechanically, TGFBR3 might promote Aβ production by enhancing β- and γ-secretase activities. Further investigation revealed that TGFBR3 may exert its functions via Synaptic vesicle cycle, Calcium signaling pathway and MAPK signal pathway by regulating hub genes GNB1, GNG3, CDC5L, DYNC1H1 and FBXW7. Overall, our findings highlighted TGFBR3 as an AD risk gene and might be used as a diagnostic biomarker and therapeutic target for AD treatment.
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http://dx.doi.org/10.3389/fcell.2021.825729DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8924075PMC
March 2022

Aronia melanocarpa (Michx.) Elliott. attenuates dextran sulfate sodium-induced Inflammatory Bowel Disease via regulation of inflammation-related signaling pathways and modulation of the gut microbiota.

J Ethnopharmacol 2022 Jun 16;292:115190. Epub 2022 Mar 16.

School of Life Science, Jilin Agricultural University, Jilin, PR China; Engineering Research Center of Chinese Ministry of Education for Edible and Medicinal Fungi, Changchun, PR China. Electronic address:

Ethnopharmacological Relevance: Aronia melanocarpa (Michx.) Elliott. Is one of the most functional berries usually used in the preparation of juice and jams, but it has revealed its ethnopharmacological properties due to their richness in biologically active molecules with pharmaceutical and physiological effects.

Aims Of The Study: The aim of this study was to assess the antioxidant and anti-inflammatory effects of Aronia melanocarpa ethanol-extract as well as the possible mechanisms of action involved and the modulation of gut microbiota in Dextran Sulfate Sodium (DSS)-induced Inflammatory bowel disease in mice.

Materials And Methods: Inflammatory bowel disease (IBD) were induced by DSS in drinking water for 7 days to evaluate the properties of A. melanocarpa ethanol-extract (AME) on the intestinal microflora. AME was administered orally to DSS-induced IBD mice for 21 days. Clinical, inflammatory, histopathological parameters, and different mRNA and proteins involved in its possible mechanism of action were determined as well as gut microbiota analysis via 16S high throughput sequencing.

Results: AME improved clinical symptoms and regulated histopathological parameters, pro- and anti-inflammatory cytokines and oxidative stress factors as well as mRNA and protein expressions of transcription factors involved in maintaining the intestinal barrier integrity. In addition, AME also reversed the DSS-induced intestinal dysbiosis effects promoting the production of cecal short chain fatty acids linked to signaling pathways inhibiting IBD.

Conclusion: AME improved intestinal lesions induced by DSS suggesting that A. melanocarpa berries could have significant therapeutic potential against IBD due to their antioxidant and anti-inflammatory capacities as well as their ability to restore the gut microbiota balance.
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http://dx.doi.org/10.1016/j.jep.2022.115190DOI Listing
June 2022

Fabrication of Bi-BiOCl plasmon photocatalysts for removal of aqueous emerging contaminants under visible light.

J Environ Sci (China) 2022 Aug 11;118:87-100. Epub 2022 Jan 11.

School of Construction and Environment Engineering, Shenzhen Polytechnic, Shenzhen 518055, China.

Photocatalytic oxidation of emerging contaminants (ECs) in water has recently gained extensive attentions. In this study, bismuth oxychloride-based plasmon photocatalysts (Bi-BiOCl) exhibiting high performance were successfully developed by reducing Bi on the surface of BiOCl. Consequently, the photocatalysts were used to remove ECs from water. The effects of developmental process and Bi metal plasmon resonance on the photoelectric performances of Bi-BiOCl were investigated through a series of characterizations. The UV-vis diffuse reflection and photoluminescence spectra revealed that the light absorption range of the photocatalyst gradually increased and the electron recombination rate gradually decreased with the introduction of Bi metals. The optimal removal rates of ciprofloxacin and tetrabromobisphenol A by Bi-BiOCl were 93.8% and 96.4%; the respective reaction rate constants were 5.48 and 4.93 times higher than that of BiOCl. The mechanism study indicated that main reactants in the photocatalytic system were •O radicals and photogenerated holes, and the existence of oxygen vacancies and Bi metals promoted electron transfer in photocatalyst. In conclusion, this research produces a novel, green, highly efficient, and stable visible light photocatalyst for the removal of ECs from water.
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http://dx.doi.org/10.1016/j.jes.2021.08.026DOI Listing
August 2022

Practice and experience of regional medical center entrance linkage and closed-loop management under the wartime situation of the COVID-19 in China.

Ann Transl Med 2022 Jan;10(2):112

Clinical Medical College, Yangzhou University, Yangzhou, China.

On 28 July 2021, the first indigenous case of novel coronavirus pneumonia (COVID-19) emerged in Yangzhou, marking the beginning of a public health crisis caused by the new coronavirus pneumonia. It is a significant challenge for hospitals to carry out prevention and control measures to ensure the safety of medical professionals and patients when facing the changes in an epidemic situation. Subei People's Hospital, as one of the first group of "Grade III-class A" hospitals in Jiangsu Province and the Yangzhou Regional Medical Centre, responded quickly and scientifically to prevent and control the disease. A closed-loop management system was implemented at the hospital entrance (consisting of the outpatient clinic, emergency clinic, fever clinic, and buffer ward) and an epidemic prevention and control group was established with the assistance of multiple departments. This group optimized the pre-screening and triage system, standardized the fever clinic consultation process, and improved the construction of an information-based prevention and control network so that patients were detected, diagnosed, isolated, and treated early. The emergency management capability was improved to achieve zero missed consultations of patients attending for COVID-19 and to effectively maintain medical order during this critical period. This current report systematically summarizes the operational practices and the effectiveness achieved by implementation of the entrance closed-loop management in the hospital and analyzed the key operational issues for future reference by medical institutions and management departments.
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http://dx.doi.org/10.21037/atm-22-61DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8848449PMC
January 2022

Glutathione-Sensitive Mesoporous Organosilica-Coated Gold Nanorods as Drug Delivery System for Photothermal Therapy-Enhanced Precise Chemotherapy.

Front Chem 2022 25;10:842682. Epub 2022 Feb 25.

Department of Clinical Laboratory, Shanghai Gongli Hospital, The Second Military Medical University, Pudong New Area, Shanghai, China.

The combination of photothermal therapy (PTT) and chemotherapy can remarkably improve the permeability of the cell membrane and reduce the concentration of chemotherapy agents that not only kill the tumor cells effectively but also have adverse effects on normal tissues. It is of great meaning to construct nanomaterials that could be simultaneously applied for tumor eradication with PTT and chemotherapy. In this work, we developed a novel gold nanorod coated with mesoporous organosilica nanoparticles (oMSN-GNR), which presented as an optimal photothermal contrast agent. Moreover, after doxorubicin loading (oMSN-GNR-DOX), the organosilica shell exhibited biodegradable properties under high glutathione in the tumor microenvironment, resulting in massively releasing doxorubicin to kill tumor cells. More importantly, the hyperthermia effect of GNR cores under near-infrared light provided promising opportunities for localized photothermal ablation . Therefore, the combination of precise chemotherapy and highly effective PTT successfully inhibited tumor growth in liver tumor-bearing mice. This versatile synergistic therapy with local heating and chemotherapeutics precise release opens up the potential clinical application of PTT and chemotherapy therapeutics for malignant tumor eradication.
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http://dx.doi.org/10.3389/fchem.2022.842682DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8914165PMC
February 2022

Correlation Analyses on Physical and Mechanical Parameters of Concrete in Marine Environments.

Materials (Basel) 2022 Feb 28;15(5). Epub 2022 Feb 28.

Hubei Key Laboratory of Engineering Structural Analysis and Safety Assessment, Department of Mechanics, School of Aerospace Engineering, Huazhong University of Science and Technology, Wuhan 430074, China.

This work explored the correlation among the physical and mechanical parameters of concrete in marine environments during corrosion. Concrete materials with varied water-cement ratios (/) were soaked in four kinds of simulated seawater, including a compound solution of sodium chloride (NaCl) and magnesium sulfate (MgSO), a solution of MgSO, a solution of sodium sulfate (NaSO) and clear water. The macroscopic physical and mechanical parameters, such as compressive strength, tensile strength, mass change and ultrasonic wave velocity, were measured. The damage mechanism of corrosion products and microstructures was analyzed using microscopic approaches including scanning electron microscopy (SEM), X-ray diffraction (XRD), etc. Linear correlation was carried out on the abovementioned physical parameters, and the results revealed that, during corrosion, the correlation between compressive and tensile strength and mass (excluding NaSO solution) is positive (mostly highly correlated), while that with wave velocity is poor (mostly moderately correlated).
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http://dx.doi.org/10.3390/ma15051812DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8912119PMC
February 2022

Repetitive Blood Sampling from the Subclavian Vein of Conscious Rat.

J Vis Exp 2022 02 9(180). Epub 2022 Feb 9.

Guangdong Lewwin Pharmaceutical Research Institute Co., Ltd., Guangdong Provincial Key Laboratory of Drug Non-Clinical Evaluation and Research, TCM Non-clinic Evaluation Branch of National Engineering Research Center for Modernization of Traditional Chinese Medicine, Guangdong Engineering Research Center for Innovative Drug Evaluation and Research;

Rats are widely used in pharmacokinetics (PK) and toxicokinetic (TK) studies that need to collect a certain amount of blood at specific time points to detect drug exposure. The rat blood sampling method determines the quality of the plasma and further affects the precision of the test results. The subclavian vein blood collection method described in this protocol collects blood samples repeatedly in the conscious state of animals to meet the needs of PK and TK tests. The skills of restraint handling and appropriate procedure of needle incision ensure the success rate of blood collection. It is easy to operate while ensuring the quality of plasma and at the same time catering to animal welfare. However, this method requires skilled operation, and an improper one may cause animal weakness, pain, lameness, and even mortality. The current method has been used in the testing facility for a 4-week oral toxicity study in Sprague Dawley (SD) rats with TK. The maximum amount of blood collected within 24 h did not exceed 20% of the animal's total blood. The animals' body weight was more than 200 g for males and females. The data showed that the animals' bodyweight increased steadily every week, and the clinical observation was normal after repetitive sample collection.
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http://dx.doi.org/10.3791/63439DOI Listing
February 2022

Glucocorticoids Inhibit EGFR Signaling Activation in Podocytes in Anti-GBM Crescentic Glomerulonephritis.

Front Med (Lausanne) 2022 10;9:697443. Epub 2022 Feb 10.

National Clinical Research Center for Kidney Diseases, Jingling Hospital, Nanjing University School of Medicine, Nanjing, China.

Glucocorticoids are commonly used to treat anti-GBM crescentic glomerulonephritis, however, the mechanism underlying its therapeutic effectiveness is not completely understood. Since podocyte EGFR/STAT3 signaling is known to mediate the development of anti-GBM glomerulonephritis, we investigated the effect of glucocorticoids on EGFR/STAT3 signaling in podocytes. We found that the levels of phosphorylated (activated) EGFR and STAT3 in podocytes were markedly elevated in anti-GBM patients without glucocorticoids treatment, but were normalized in patients with glucocorticoids treatment. In a rat model of anti-GBM glomerulonephritis, glucocorticoids treatment significantly attenuated the proteinuria, crescent formation, parietal epithelial cell (PEC) activation and proliferation, accompanied by elimination of podocyte EGFR/STAT3 signaling activation. In cultured podocytes, glucocorticoids were found to inhibit HB-EGF-induced EGFR and STAT3 activation. The conditioned medium from podocytes treated with HB-EGF in the absence but not presence of glucocorticoids was capable of activating Notch signaling (which is known to be involved in PEC proliferation and crescent formation) and enhancing proliferative activity in primary PECs, suggesting that glucocorticoids prevent podocytes from producing secreted factors that cause PEC proliferation and crescent formation. Furthermore, we found that glucocorticoids can downregulate the expression of EGFR ligands, EGF and HB-EGF, while upregulate the expression of EGFR inhibitor, Gene 33, explaining how glucocorticoids suppress EGFR signaling. Taken together, glucocorticoids exert therapeutic effect on anti-GBM crescentic glomerulonephritis through inhibiting podocyte EGFR/STAT3 signaling and the downstream pathway that leads to PEC proliferation and crescent formation.
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http://dx.doi.org/10.3389/fmed.2022.697443DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8866651PMC
February 2022

Calcar fracture gapping: a reliable predictor of anteromedial cortical support failure after cephalomedullary nailing for pertrochanteric femur fractures.

BMC Musculoskelet Disord 2022 Feb 24;23(1):175. Epub 2022 Feb 24.

Department of Orthopaedic Surgery, Yangpu Hospital, School of Medicine, Tongji University, Shanghai, 200090, China.

Background: Maintaining anteromedial cortical support is essential for controlling sliding and decreasing postoperative implant-related complications. However, adequate fracture reduction with cortical support in immediate postoperative fluoroscopy is not invariable in postoperative follow-ups. This study was conducted to investigate the risk factors leading to anteromedial cortical support failure in follow up for pertrochanteric femur fractures treated with cephalomedullary nails.

Methods: This retrospective study enrolled 159 patients with pertrochanteric fractures (AO/OTA- 31A1 and 31A2) that fixed with cephalomedullary nails. All patients were evaluated as adequate fracture reduction in immediate postoperative fluoroscopy before leaving the operation theater. The patients were separated into two groups based on the condition of the anteromedial cortex in the postoperative 3D CT with full-range observation: those with calcar support maintained in Group 1 and those with calcar support lost in Group 2. Demographic information, fracture classification, TAD (tip-apex distance), Cal-TAD, Parker ratio, NSA (neck-shaft angle), reduction quality score, and calcar fracture gapping were collected and compared. Logistic regression analysis was conducted to explore the risk factors leading to anteromedial cortex change.

Results: Anteromedial cortical support failure was noted in 46 cases (29%). There was no significant difference between the two groups concerning age, sex, side injury, TAD, Cal-TAD, Parker ratio, or NSA. There was a significant difference in the AO/OTA fracture classification in univariate analysis but no difference in the multivariable analysis. The reduction quality score, calcar fracture gapping in the AP (anteroposterior), and lateral views were significantly associated with anteromedial cortical support failure in follow-up after cephalomedullary nailing in the multivariable analysis. The threshold value of calcar fracture gapping for the risk of loss was 4.2 mm in the AP and 3.8 mm in the lateral fluoroscopies. Mechanical complications (lateral sliding and varus) were frequently observed in the negative anteromedial cortical support group.

Conclusions: Good reduction quality was a protective factor, and larger calcar fracture gapping in the AP and lateral views were risk factors leading to the postoperative loss of anteromedial cortical support. Therefore, we should pay close attention to fracture reduction and minimize the calcar fracture gap during surgery.
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http://dx.doi.org/10.1186/s12891-021-04873-7DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8876129PMC
February 2022

Comparative transcriptome analysis provides insights into grain filling commonalities and differences between foxtail millet [ (L.) P. Beauv.] varieties with different panicle types.

PeerJ 2022 18;10:e12968. Epub 2022 Feb 18.

Anyang Academy of Agriculture Sciences, Anyang, Henan, China.

Grain filling affects grain weight and quality and is among the most critical factors in determining the yield and quality of cereal crops. Though hybrids have larger panicles and numerous spikelets with a larger sink capacity than conventional varieties, data on the grain filling commonalities and differences between foxtail millet varieties with different panicle types remain sparse. In this study, we found that "Zhang Gu 13" (ZG, large panicle) exhibits a significantly higher panicle weight than "Yu Gu 18" (YG, conventional panicle) at the early stage of grain filling, but the weight of YG increased rapidly and gradually overtook ZG during the middle stages. A temporal expression pattern analysis demonstrated that the genes involved in photosynthesis, metabolic pathways, and phenylpropanoid biosynthesis were downregulated, while those related to peroxisome function, purine metabolism, and zeatin biosynthesis were upregulated during grain filling in both varieties. A total of 6,832 differentially expressed genes (DEGs) were identified in both varieties, with the majority identified at the early and late stages. A Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis further revealed that the upregulated DEGs in YG were associated with gibberellin (GA) biosynthesis, ATP-binding cassette (ABC) transporters, and plant hormone signal transduction. Photosynthesis-related DEGs, such as photosystem and antenna proteins, were significantly upregulated in ZG. This study provides preliminary insights into the differences in gene expression and molecular mechanisms of grain filling between ZG and YG in the North China summer-sowing region.
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http://dx.doi.org/10.7717/peerj.12968DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8860066PMC
February 2022

Integrated comparison of the mRNAome in cartilage, synovium, and macrophages in osteoarthritis.

Authors:
Siming Gao Hui Song

Z Rheumatol 2022 Feb 17. Epub 2022 Feb 17.

Department of Rheumatology, Beijing Jishuitan Hospital, No. 31, Xin Jie Kou East Street, Xicheng District, 100035, Beijing, China.

The precise molecular mechanisms associated with osteoarthritis (OA), the most common musculoskeletal disorder, are poorly understood. There are currently no effective treatments to prevent the initiation and progression of the disease. In recent years, the development of mRNAome has made it possible to identify new mechanisms and therapeutic targets. However, the differentially expressed genes screened by different microarrays are not completely the same. In order to avoid this shortcoming, we integrate the different genes from different tissues and data sets, and select the commonly expressed genes for further studies.
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http://dx.doi.org/10.1007/s00393-022-01171-yDOI Listing
February 2022
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