Publications by authors named "Hui Song"

453 Publications

Generation and Characterization of Mouse Models of C3 Glomerulonephritis With CFI D288G and P467S Mutations.

Front Physiol 2021 3;12:649801. Epub 2021 Jun 3.

National Clinical Research Center for Kidney Diseases, Affiliated Jinling Hospital, Medical School of Nanjing University, Nanjing, China.

C3 glomerulopathy (C3GP) is a disease entity caused by abnormality of the complement alternative pathway (AP) and characterized by C3 deposition in glomeruli. Many variations or mutations of complement factors are believed to underlie the susceptibility to C3GP, but there is a lack of experimental evidence. We have recently reported a patient with C3 glomerulonephritis (C3GN) and compound heterozygosity of two novel variations in the complement factor (CFI). Here, we generated a mouse model to mimic the CFI variations for studying pathogenicity of CFI variations in C3GN development. We used the CRISPR/Cas9 system to make mutant mouse lines that carried D288G and P467S mutations in CFI, respectively, and crossed them to generate mice with compound heterozygosity of CFI D288G and P467S. The mice were all normal in either SPF (specific pathogen free) or regular environment. When treated with lipopolysaccharides (LPS), a bacterial endotoxin that mimics infection and sepsis, the mice developed albuminuria, kidney function impairment, and C3 glomerular deposition at levels comparable with the wild-type mice. The mice with other genotypes concerning CFI D288G and P467S were also tested in parallel. Unexpectedly, we found that the D288G homozygotes all developed severe mesangial deposition of C3 in the LPS model, indicating that CFI D288G variation was involved in the C3 deposition, a key feature of C3GN. The mouse lines generated in the present study can be used to further study the role of CFI variations in C3GN development; in addition, they may be used to screen and test infections and environmental factors capable of triggering C3GN.
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http://dx.doi.org/10.3389/fphys.2021.649801DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8209374PMC
June 2021

A mesoporous non-precious metal boride system: synthesis of mesoporous cobalt boride by strictly controlled chemical reduction.

Chem Sci 2019 Nov 15;11(3):791-796. Epub 2019 Nov 15.

School of Chemical Engineering, Australian Institute for Bioengineering and Nanotechnology (AIBN), The University of Queensland Brisbane Queensland 4072 Australia

Generating high surface area mesoporous transition metal boride is interesting because the incorporation of boron atoms generates lattice distortions that lead to the formation of amorphous metal boride with unique properties in catalysis. Here we report the first synthesis of mesoporous cobalt boron amorphous alloy colloidal particles using a soft template-directed assembly approach. Dual reducing agents are used to precisely control the chemical reduction process of mesoporous cobalt boron nanospheres. The Earth-abundance of cobalt boride combined with the high surface area and mesoporous nanoarchitecture enables solar-energy efficient photothermal conversion of CO into CO compared to non-porous cobalt boron alloys and commercial cobalt catalysts.
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http://dx.doi.org/10.1039/c9sc04498aDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8145993PMC
November 2019

Complete chloroplast genome sequence of .

Mitochondrial DNA B Resour 2021 May 31;6(7):1814-1815. Epub 2021 May 31.

CAS Key laboratory of Mountain Ecological Restoration and Bioresource Utilization & Ecological Restoration and Biodiversity Conservation Key Laboratory of Sichuan Province, Chengdu Institute of Biology, Chinese Academy of Sciences, Chengdu, China.

The complete chloroplast genome of was reconstructed by reference-based assembly using Illumina paired-end data. The assembled plastome is 153,234 base pairs (bp) in length, including a pair of inverted repeat regions (IRs) of 22,662 bp each, a large single-copy region (LSC) of 89,059 bp and a small single-copy region (SSC) of 19,651 bp. A total of 115 genes were predicted from the chloroplast genome, including 74 protein coding genes, 37 tRNA genes and 4 rRNA genes. The overall GC content of chloroplast genome was 37.7%. Phylogenetic analysis with several reported chloroplast genomes showed that is closely clustered with . The complete chloroplast genome of provides new insight into the evolutionary and genomic studies of Convolvulaceae.
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http://dx.doi.org/10.1080/23802359.2021.1915202DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8168749PMC
May 2021

Chemical characterization of PM emitted from China IV and China V light-duty vehicles in China.

Sci Total Environ 2021 Aug 18;783:147101. Epub 2021 Apr 18.

Xi'an Environmental Protection Bureau, Xi'an 710054, China.

This study reported the emission factors (EFs) and detailed chemical compositions of PM collected from China IV and China V light-duty vehicles (LDVs) through dynamometer test. The China IV LDVs containing 4 gasoline vehicles (GVs) and 4 natural gas vehicles (NGVs) had port fuel injection (PFI) engines, while the China V LDVs included 2 GVs with PFI engines and 2 GVs with gasoline direct injection (GDI) engines. The average EFs of PM were 1.90 ± 0.70 mg km, 1.44 ± 0.29 mg km, and 0.56 ± 0.05 mg km for China IV GVs, China IV NGVs, and China V GVs, respectively. PM profiles of LDVs were characterized by abundant carbon species (60.59-68.58%) with low amounts of water soluble ions (WSIs, 6.96-16.37%) and elements (5.20-7.53%). In general, the EFs of PM constituents including organic carbon (OC), elemental carbon (EC), WSIs, and elements were reduced obviously by strengthening emission standards from China IV to China V. While the contributions of most WSIs and elements to PM increased as vehicle technology improved. Furthermore, the EFs of PM components from China IV LDVs also decreased when shifting fuels from gasoline to natural gas. While the fractions of OC, WSIs and most elements in PM increased due to the highest reduction rate of EC mass. For China V LDVs, GDI vehicles emitted less OC but more EC compared to PFI vehicles, and the EFs of most WSIs and elements also increased. Overall, GDI vehicles exhibited lower fractions OC and WSIs but higher contents of EC and elements in PM. Besides, PM and its chemical species were heavily dependent on vehicle's driving patterns. The average EFs of PM components under aggressive driving pattern increased significantly compared to those under moderate driving pattern.
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http://dx.doi.org/10.1016/j.scitotenv.2021.147101DOI Listing
August 2021

Comparison of the visual performance of iris-fixated phakic lens and implantable collamer lens to correct high myopia.

BMC Ophthalmol 2021 Jun 2;21(1):244. Epub 2021 Jun 2.

Tianjin Eye Hospital, Tianjin Key Lab of Ophthalmology and Visual Science, Nankai University Affiliated Eye Hospital, Clinical College of Ophthalmology Tianjin Medical University, Tianjin Eye Institute, No. 4 Gansu Road, Heping District, Tianjin, 300020, China.

Background: To compare visual performance between the iris-fixated phakic intraocular len (pIOL) and implantable collamer len (ICL) to correct high myopia.

Methods: Twenty-four eyes underwent iris-fixated pIOL implantation and 24 eyes underwent ICL implantation. At the 6-month follow-up, the best-corrected visual acuity (BCVA) and uncorrected distance visual acuity (UDVA) were compared between the iris-fixated pIOL and ICL groups. The objective scatter index (OSI), modulation transfer function (MTF) cutoff, and ocular aberrations were performed to evaluate postoperative visual quality between the two groups.

Results: No significant difference was found in UDVA, BCVA, and spherical equivalent between the iris-fixated pIOL and ICL groups (P > 0.05). Six months after surgery, the following values were significantly higher in the ICL group than in the iris-fixated pIOL group: MTF cutoff, strehl ratio and optical quality analysis system values at contrasts of 9 %, 20 %, and 100 % (P < 0.01). The OSI in the iris-fixated pIOL group was higher than in the ICL group 6 months after surgery (P < 0.01). All high-order aberrations were slightly more severe in the iris-fixated pIOL group than in the ICL group 6 months after surgery, although only trefoil (P = 0.023) differed significantly in this regard.

Conclusions: Both iris-fixated lenses and ICLs can provide good visual acuity. ICLs confer better visual performance in MTF-associated parameters and induce less intraocular light scattering than iris-fixated pIOLs.
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http://dx.doi.org/10.1186/s12886-021-01995-3DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8173970PMC
June 2021

Clinical Efficacy of Patch in Patients With Symptomatic Knee Osteoarthritis: A Multicenter Non-Inferiority Randomized Controlled Clinical Trial.

Front Pharmacol 2021 7;12:646310. Epub 2021 May 7.

Department of Rheumatology, Guang'anmen Hospital, China Academy of Chinese Medical Sciences, Beijing, China.

Osteoarthritis (OA) is imposing substantial burdens on individuals and society with the aging population. Cortex Daphnes patch is widely used for symptomatic knee OA in China with a satisfying clinical efficacy; however, there is scant clinical evidence supporting its use. To evaluate its efficacy, we conducted a multicenter, non-inferiority, randomized, parallel-group study comparing Cortex Daphnes patch with topical nonsteroidal anti-inflammatory drugs in patients with knee OA (NCT02770950). A total of 264 symptomatic knee OA patients were treated with Cortex Daphnes or indomethacin cataplasms applied to affected sites once daily for 2 weeks. The primary outcome was improvement in knee pain on walking as assessed using a visual analog scale (VAS). The non-inferiority margin based on the full analysis population was set as -5 mm on the pain VAS. The secondary outcomes were changes of the Western Ontario and McMaster Universities Osteoarthritis Index (WOMAC) total score, WOMAC scores for pain, function and stiffness, the 36-item Short Form Health Survey (SF-36), and global assessment of knees by the patients. Responder rates for pain VAS, WOMAC total score, and WOMAC pain were also included in the secondary outcomes. The Cortex Daphnes patch was non-inferior to indomethacin cataplasms for the primary outcome with a group difference (Cortex Daphnes patch-indomethacin cataplasm) of 2.1 mm (95% confidence interval: 2.1-6.4); similar results were found in the per-protocol population. For all other outcomes, no significant differences were found in the full analysis set or in the per-protocol analysis set, except the responder rates for WOMAC pain was higher in the Cortex Daphnes patch group than in the indomethacin cataplasm group (78.4 vs. 64.7%, p = 0.022) in the per-protocol analysis set. Overall, 28.8% patients in the Cortex Daphnes patch group and 9.8% in the indomethacin cataplasm group reported treatment-related adverse events, the vast majority of which were mild-to-moderate skin irritation, resulting in only 3.8 and 0.8% of patients dropping out, respectively. The Cortex Daphnes patch, which provides satisfactory analgesic efficacy and enhances the physical function of the knee, as well as improving quality of life, may be a promising alternative to knee OA.
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http://dx.doi.org/10.3389/fphar.2021.646310DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8137983PMC
May 2021

[Interaction of IL-6 gene with tumor necrosis factor-α and white blood cell in metabolic syndrome].

Wei Sheng Yan Jiu 2021 Mar;50(2):261-273

School of Public Health, Ningxia Medical University, Yinchuan 750004, China.

Objective: To investigate the associations between the polymorphisms and interaction of the interleukin 6(IL-6) genes at-634 C/G, -174 G/C, -1363 G/T loci, as well as the interactions between the three loci and tumor necrosis factor(TNF-α), and peripheral blood leukocytes(white blood cell, WBC) with metabolic syndrome(MS).

Methods: Using the case-control research method, according to the inclusion and exclusion criteria, from March 2015 to March 2016 from the General Hospital of Ningxia Medical University and Wuzhong City People's Hospital, 376 unrelated cases and 408 control groups were selected. We conducted questionnaire surveys(including general conditions, disease and medication history, family history, etc. ), physical examinations(including height, weight and calculation of body mass index(BMI), waist circumference, hip circumference and calculation of waist-to-hip ratio(WHR), SBP, DBP, etc. ), blood collection and testing(including WBC count, serum TNF-α level and biochemical indicators TG, TC, HDL-C, LDL-C, FPG, UA, AST, ALT, etc. ), and the polymorphism detection of IL-6 gene at-634 C/G, -174 G/C, -1363 G/T sites. The SNPstats online software was used to analyze the relationship between the polymorphisms of IL-6 gene and MS. The SHEsis online software was used to analyze the linkage disequilibrium(LD) of the IL-6 three-site and the relationship between haplotype and MS. GMDR 0. 7 software was used to analyze the interactions among the three sites of IL-6 gene, and one between the three sites and TNF-α and WBC, respectively.

Results: Before and after adjustment of sex, age and nationality, the polymorphism at the 3 position of IL-6 gene was not related to the onset of MS in different genetic models(both P> 0. 05). There was a linkage disequilibrium between the three loci of IL-6 gene, but the haploids formed by these three loci were not associated with MS susceptibility(all P> 0. 05). There was no interaction among the three sites, but the two-factor, three-factor, and four-factor interaction models consisting of the three sites and TNF-α were all statistically significant(all P<0. 001) and the replacement tests were all P<0. 001, and were all associated with MS occurrence. The two-factor, three-factor, and four-factor interaction models consisting of the three sites and WBC were all P<0. 01, and the replacement tests were all P<0. 05. The differences were statistically significant, which was related to the onset of MS.

Conclusion: The IL-6 gene-634 C/G, -174 G/C, and-1363 G/T loci polymorphism may not be significantly associated with the prevalence of MS. Interactions between the three sites and TNF-α and WBC levels can significantly increase the risk of MS.
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http://dx.doi.org/10.19813/j.cnki.weishengyanjiu.2021.02.016DOI Listing
March 2021

CUL4B facilitates HBV replication by promoting HBx stabilization.

Cancer Biol Med 2021 May 9. Epub 2021 May 9.

Ministry of Education Key Laboratory of Experimental Teratology and Institute of Molecular Medicine and Genetics, School of Basic Medical Sciences, Shandong University, Jinan 250012, China.

Objective: Hepatitis B virus (HBV) infection is a major public health problem worldwide. However, the regulatory mechanisms underlying HBV replication remain unclear. Cullin 4B-RING ubiquitin E3 ligase (CRL4B) is involved in regulating diverse physiological and pathophysiological processes. In our study, we aimed to explain the role of CUL4B in HBV infection.

Methods: transgenic mice or conditional knockout mice, as well as liver cell lines with CUL4B overexpression or knockdown, were used to assess the role of CUL4B in HBV replication. Immunoprecipitation assays and immunofluorescence staining were performed to study the interaction between CUL4B and HBx. Cycloheximide chase assays and ubiquitination assays were performed to evaluate the half-life and the ubiquitination status of HBx.

Results: The hydrodynamics-based hepatitis B model in transgenic or conditional knockout mice indicated that CUL4B promoted HBV replication ( < 0.05). Moreover, the overexpression or knockdown system in human liver cell lines validated that CUL4B increased HBV replication in an HBx-dependent manner. Importantly, immunoprecipitation assays and immunofluorescence staining showed an interaction between CUL4B and HBx. Furthermore, CUL4B upregulated HBx protein levels by inhibiting HBx ubiquitination and proteasomal degradation ( < 0.05). Finally, a positive correlation between CUL4B expression and HBV pgRNA level was observed in liver tissues from HBV-positive patients and HBV transgenic mice.

Conclusions: CUL4B enhances HBV replication by interacting with HBx and disrupting its ubiquitin-dependent proteasomal degradation. CUL4B may therefore be a potential target for anti-HBV therapy.
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http://dx.doi.org/10.20892/j.issn.2095-3941.2020.0468DOI Listing
May 2021

Galectin-9 Targets NLRP3 for Autophagic Degradation to Limit Inflammation.

J Immunol 2021 Jun 7;206(11):2692-2699. Epub 2021 May 7.

Department of Immunology, Key Laboratory for Experimental Teratology of the Chinese Ministry of Education, School of Basic Medical Science, Cheeloo College of Medicine, Shandong University, Jinan, Shandong, China;

NOD-, LRR-, and pyrin domain-containing protein 3 (NLRP3) inflammasome has been implicated in a variety of inflammatory disorders, and its activation should be tightly controlled to avoid detrimental effects. NLRP3 protein expression is considered as the rate-limiting step for NLRP3 inflammasome activation. In this study, we show that galectin-9 (encoded by ) attenuated NLRP3 inflammasome activation by promoting the protein degradation of NLRP3 in primary peritoneal macrophages of C57BL/6J mice. deficiency enhances NLRP3 inflammasome activation and promotes NLRP3-dependent inflammation in C57BL/6J mice in vivo. Mechanistically, galectin-9 interacts with NLRP3, promotes the formation of NLRP3/p62 (an autophagic cargo receptor, also known as SQSTM1) complex, and thus facilitates p62-dependent autophagic degradation of NLRP3 in primary peritoneal macrophages of C57BL/6J mice and HEK293T cells. Therefore, we identify galectin-9 as an "eat-me" signal for selective autophagy of NLRP3 and uncover the potential roles of galectins in controlling host protein degradation. Furthermore, our work suggests galectin-9 as a priming therapeutic target for the diseases caused by improper NLRP3 inflammasome activation.
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http://dx.doi.org/10.4049/jimmunol.2001404DOI Listing
June 2021

Carbon Monoxide Releasing Molecule-3 Enhances Osteogenic Differentiation of Human Periodontal Ligament Stem Cells by Carbon Monoxide Release.

Drug Des Devel Ther 2021 22;15:1691-1704. Epub 2021 Apr 22.

Department of VIP Center, School and Hospital of Stomatology, Cheeloo College of Medicine, Shandong University & Shandong Key Laboratory of Oral Tissue Regeneration & Shandong Engineering Laboratory for Dental Materials and Oral Tissue Regeneration, Jinan, 250012, Shandong, People's Republic of China.

Purpose: Limited intrinsic regeneration capacity following bone destruction remains a significant medical problem. Multiple regulatory effects of carbon monoxide releasing molecule-3 (CORM-3) have been reported. The aim of this study was to investigate the effect of CORM-3 on the osteogenic differentiation of human periodontal ligament stem cells (hPDLSCs) during osteogenesis.

Patients And Methods: hPDLSCs obtained from healthy periodontal ligament tissues were cultured and identified with specific surface antigens by flow cytometry. Effect of CORM-3 on the proliferation of hPDLSCs was determined by CCK-8 assay. Alizarin red staining and alkaline phosphatase (ALP) activity were used to assess the osteogenic differentiation of hPDLSCs. Real-time quantitative polymerase chain reaction (RT-qPCR) and Western blot analysis were used to detect the expression of the indicated genes. Critical-sized skull defect was made in Balb/c-nude mice, microcomputed tomography (Micro-CT) and Masson trichrome staining were used to assess the new bone regeneration in mice.

Results: CORM-3 (400 μmol/l) significantly promoted the proliferation of hPDLSCs. CORM-3 pretreatment not only notably enhanced the mRNA and protein expression of osteo-specific marker OPN, Runx2 and ALP, but also increased mineral deposition and ALP activity by the release of CO on day 3, 7 and 14 (P<0.05). Degassed CORM-3 did not show the same effect as CORM-3. In animal model, application of CORM-3 with hPDLSCs transplantation highly increased new bone formation in skull defect region.

Conclusion: CORM-3 promoted osteogenic differentiation of hPDLSCs, and increased hPDLSCs-induced new bone formation in mice with critical-sized skull defect, which suggests an efficient and promising strategy in the treatment of disease with bone defect.
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http://dx.doi.org/10.2147/DDDT.S300356DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8075314PMC
April 2021

Chemogenetic stimulation of proprioceptors remodels lumbar interneuron excitability and promotes motor recovery after SCI.

Mol Ther 2021 Apr 23. Epub 2021 Apr 23.

Department of Orthopedics, The Second Affiliated Hospital of Xi'an Jiaotong University, Xi'an, Shaanxi 710004, China; Department of Orthopedics, Xi'an International Medical Center, Xi'an, Shaanxi 710075, China. Electronic address:

Motor recovery after severe spinal cord injury (SCI) is limited due to the disruption of direct descending commands. Despite the absence of brain-derived descending inputs, sensory afferents below injury sites remain intact. Among them, proprioception acts as an important sensory source to modulate local spinal circuits and determine motor outputs. Yet, it remains unclear whether enhancing proprioceptive inputs promotes motor recovery after severe SCI. Here, we first established a viral system to selectively target lumbar proprioceptive neurons and then introduced the excitatory Gq-coupled Designer Receptors Exclusively Activated by Designer Drugs (DREADD) virus into proprioceptors to achieve specific activation of lumbar proprioceptive neurons upon CNO administration. We demonstrated that chronic activation of lumbar proprioceptive neurons promoted the recovery of hindlimb stepping ability in a bilateral hemisection SCI mouse model. We further revealed that chemogenetic proprioceptive stimulation led to coordinated activation of proprioception-receptive spinal interneurons and facilitated transmission of supraspinal commands to lumbar motor neurons, without affecting the regrowth of proprioceptive afferents or brain-derived descending axons. Moreover, application of 4-aminopyridine-3-methanol (4-AP-MeOH) that enhances nerve conductance further improved the transmission of supraspinal inputs and motor recovery in proprioception-stimulated mice. Our study demonstrates that proprioception-based combinatorial modality may be a promising strategy to restore the motor function after severe SCI.
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http://dx.doi.org/10.1016/j.ymthe.2021.04.023DOI Listing
April 2021

Analysis of the microRNA and mRNA expression profile of ricin toxin-treated RAW264.7 cells reveals that miR-155-3p suppresses cell inflammation by targeting GAB2.

Toxicol Lett 2021 Sep 15;347:67-77. Epub 2021 Apr 15.

College of Life Science, Jilin Agricultural University, Changchun, 130118, PR China. Electronic address:

Ricin toxin (RT) is one of the most lethal toxins derived from the seed of castor beans. In addition to its main toxic mechanism of inhibiting the synthesis of cellular proteins, RT can induce the production of inflammatory cytokines. MicroRNAs (miRNAs) play a key role in regulating both innate and adaptive immunity. To elucidate the regulation of miRNAs in RT-induced inflammation injury, the RNA high-throughput sequencing (RNA-Seq) technology was used to analyze the expression profile of miRNAs and mRNAs in RT-treated RAW264.7 cells. Results showed that a total of 323 mRNAs and 19 miRNAs differentially expressed after RT treated. Meanwhile, 713 miRNA-mRNA interaction pairs were identified by bioinformatics analysis. KEGG (Kyoto Encyclopedia of Genes and Genomes) pathway analysis showed that those interaction pairs were mainly involved in JAK-STAT, T cell receptor, and MAPK signaling pathways. Moreover, we further predicted and determined the targeting relationship between miR-155-3p and GAB2 through TargetScan and dual-luciferase reporter assay. Mechanically, overexpression of miR-155-3p can reduce the secretion of TNF-α in RAW264.7 cells, revealing a possible mechanism of miR-155-3p regulating RT-induced inflammatory injury. This study provides a new perspective for clarifying the mechanism of RT-induced inflammatory injury and reveals the potential role of miRNAs in innate immune regulation.
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http://dx.doi.org/10.1016/j.toxlet.2021.04.011DOI Listing
September 2021

Visfatin Regulates Inflammatory Mediators in Mouse Intestinal Mucosa Through Toll-Like Receptors Signaling Under Lipopolysaccharide Stress.

Arch Immunol Ther Exp (Warsz) 2021 Apr 15;69(1):11. Epub 2021 Apr 15.

College of Animal Science and Veterinary Medicine, Huazhong Agricultural University, Wuhan, 430070, China.

Visfatin is a multifunctional protein involved in inflammatory immune stress. The aim of current study was to explore the role of visfatin in lipopolysaccharide (LPS)-induced intestinal mucosal inflammation and to confirm its cellular effect in inflammatory immune response through silencing of Toll-like receptors (TLRs). We divided Kunming mice into three groups: Saline group, LPS group, and LPS + visfatin group and performed hematoxylin and eosin staining, immunohistochemistry, quantitative polymerase chain reaction, Western blot, enzyme linked immunosorbent assay and RNA-seq analysis. Pretreatment of visfatin improves LPS-stimulated reduction of tight junction protein 1 (ZO-1) and secretory immunoglobulin A, inhibits overexpression of Claudin-1 and vascular endothelial growth factor, and reduces intestinal mucosal damage and inflammation. RNA-seq analysis of cellular transcriptomes indicated that visfatin is involved in down-regulation of mRNA level of TLR4 as well as attenuation of protein levels of TLR8 and nucleotide-binding oligomerization domain-containing protein 2, revealing that visfatin could reduce intestinal mucosal inflammation through TLR signaling pathway in mice ileum. In RAW264.7 cells, the genes silencing of Toll/IL-1R family, such as TLR4, TLR2, and IL-1R1, was accompanied by decreased expressions of inflammatory factors (TNF-α, IL-1β, IL-6 and MCP-1) along with lower cellular visfatin levels. Hence, visfatin maintains the intestinal mucosal barrier structure and attenuates the intestinal mucosal inflammation through the TLR signaling pathway. Likewise, the Toll/IL-1R family regulates the release of visfatin, which can participate in the inflammatory reaction through the regulation of inflammatory factors.
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http://dx.doi.org/10.1007/s00005-021-00611-yDOI Listing
April 2021

Combining 1H-NMR-based metabonomics and network pharmacology to dissect the mechanism of antidepression effect of Milletia speciosa Champ on mouse with chronic unpredictable mild stress-induced depression.

J Pharm Pharmacol 2021 Jun;73(7):881-892

Pharmaceutical College, Guangxi Medical University, Nanning, China.

Objectives: Milletia speciosa Champ (MS), a traditional Chinese medicine, has the abilities of antistress, antifatigue, anti-oxidation and so on. In our previous study, MS was found to antidepression while the underlying mechanism of which needs further elucidation.

Methods: Here, a proton nuclear magnetic resonance (1H-NMR)-based metabonomics combined network pharmacology research approach was performed to investigate the antidepressive mechanism of MS act on mouse with chronic unpredictable mild stress-induced depression.

Key Findings: Results showed that MS could alleviate the ethology of depression (including sucrose preference degree, crossing lattice numbers and stand-up times) and disordered biochemical parameters (5-hydroxytryptamine, norepinephrine and brain-derived neurotrophic factor). Metabonomics study and network pharmacology analysis showed that MS might improve depression through synergistically regulating five targets including Maoa, Maob, Ache, Ido1 and Comt, and three metabolic pathways such as tryptophan metabolism, synthesis of neurotransmitter and phospholipid metabolism.

Conclusions: This study for the first time preliminary clarified the potential antidepressive mechanism of MS and provided theoretical basis for developing MS into novel effective antidepressant.
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http://dx.doi.org/10.1093/jpp/rgaa010DOI Listing
June 2021

Biomechanical study of Tile C3 pelvic fracture fixation using an anterior internal system combined with sacroiliac screws.

J Orthop Surg Res 2021 Mar 27;16(1):225. Epub 2021 Mar 27.

Orthopedic Trauma, University of Chinese Academy of Sciences Shenzhen Hospital, Guangdong, Shenzhen, People's Republic of China.

Background: How to perform minimally-invasive surgery on Tile C pelvic fractures is very difficult, and it is also a hot topic in orthopedic trauma research. We applied minimally-invasive treatment using an anterior internal fixator combined with sacroiliac screws.

Objectives: To compare the biomechanical properties of different fixation models in pelvic facture specimens, using an internal fixation system or a steel plate combined with sacroiliac screws.

Methods: Sixteen fresh adult cadaver pelvic specimens were randomly separated into four groups named A, B, C, and D. The four groups were respectively stabilized using a two-screwed, three-screwed, or four-screwed anterior internal fixator or a steel plate with sacroiliac screws. All models were tested in both standing and sitting positions. Vertical loads of 600 N were applied increasingly. Shifts of bilateral sacroiliac joints and pubis rupture were measured.

Results: The shifts in sacroiliac joints and pubis rupture in the standing position were all less than 3.5 mm, and the shifts in the sitting position were all less than 1 mm. In the standing position, the results of shifts in the sacroiliac joints were group C < group D < group B < group A. For comparisons between A:B and C:D, P > 0.05. For comparisons between A, B:C, and D, P < 0.05. The results of shifts in pubis ruptures were group D < group C < group B < group A. In the comparison between C:D, P > 0.05; for comparisons between A:B, A:C, A:D, B:C, and B:D, P < 0.05. In the sitting posture, the results of shifts in the sacroiliac joints were group C < group D < group B < group A, and the shifts in the pubis ruptures were group D < group C < roup B < group A. For comparison between C:D, P > 0.05. For comparisons between A:B, A:C, A:D, B:C, and B:D, P < 0.05.

Conclusion: Use of an anterior internal fixator combined with sacroiliac screws effectively stabilized Tile C3 pelvic fractures. The stability of specimens increased as the number of screws in the internal fixator increased.
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http://dx.doi.org/10.1186/s13018-021-02348-yDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8004429PMC
March 2021

Carbon Monoxide-Releasing Molecule-3 Regulates the Polarization of Lipopolysaccharide-Induced Macrophages.

Inflammation 2021 Mar 22. Epub 2021 Mar 22.

Department of Vip Center, School and Hospital of Stomatology & Shandong Provincial Key Laboratory of Oral Tissue Regeneration & Shandong Engineering Laboratory for Dental Materials and Oral Tissue Regeneration, Cheeloo College of Medicine, Shandong University, No.44-1 Wenhua Road West, Jinan, 250012, Shandong, China.

Macrophages show two main phenotypes, the M1-type (pro-inflammatory) and the M2-type (anti-inflammatory). The purpose of this research was to investigate the regulatory effect of carbon monoxide releasing molecule-3 (CORM-3) on LPS-induced macrophage polarization. LPS-induced RAW264.7 cells were exposed to CORM-3 for 24 h. Polarization of cells was checked by flow cytometry; expression of M1 or M2 macrophage-related factors and NF-κB signaling factors was examined by RT-PCR, ELISA, and Western blot. Male C57 mice were divided into three groups: normal group; periodontitis group, where experimental periodontitis was established in mice; LPS+CORM-3 group, where mice with experimental periodontitis were treated with CORM-3. Polarization of macrophages and the expression of M1 or M2 macrophage-related factors were detected by immunofluorescence, ELISA, and RT-PCR. CORM-3 significantly reduced M1 macrophage proportion, but increased M2 proportion in LPS-stimulated cells. Accordingly, CORM-3 significantly suppressed the expression of M1 macrophage-related TNF-α, iNOS, IL-1β, and IL-6, but promoted M2-related IL-10 and Arg-1. The expression of p-p65, p-p50, and p-IκB induced with LPS was inhibited by CORM-3. In vivo experiments indicated that CORM-3 induced more M2 macrophages in periodontal tissues in mice with experimental periodontitis. The expression of M1 macrophage-related factor in periodontitis was inhibited, but the expression of M2-related factors was increased by CORM-3. CORM-3 inhibits macrophage polarization to pro-inflammatory M1-type and promotes to anti-inflammatory M2-type, which provides scientific basis for the application of CORM-3 in the treatment of periodontitis.
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http://dx.doi.org/10.1007/s10753-021-01450-xDOI Listing
March 2021

[Effectiveness of proximal femoral nail anti-rotation for high plane intertrochanteric femur fractures].

Zhongguo Xiu Fu Chong Jian Wai Ke Za Zhi 2021 Mar;35(3):307-311

Department of Orthopedics, Yangpu Hospital, Tongji University, Shanghai, 200090, P.R.China.

Objective: To evaluate the effectiveness of proximal femoral nail anti-rotation (PFNA) in treatment of high plane intertrochanteric femur fractures.

Methods: A retrospective analysis was performed on 33 patients who underwent closed reduction and PFNA fixation for high plane intertrochanteric femur fracture between January 2016 and June 2019. There were 12 males and 21 females with an average age of 75.1 years (mean, 47-89 years). The fractures were caused by falling from height in 21 cases, by traffic accident in 7 cases, and by other injuries in 5 cases. Fractures were classified as type A in 14 cases and type B in 19 cases according to self-defined fracture classification criteria; and as type 31-A1.2 in 14 cases and as type 31-A2.2 in 19 cases according to AO/Orthopedic Trauma Association (AO/OTA) classification criteria. The time from injury to operation was 2-5 days (mean, 2.7 days). The operation time, intraoperative blood loss, hospital stay, fracture reduction quality, fracture healing time, internal fixation failure, and Parker-Palmer score were recorded.

Results: The operation time was 40-75 minutes (mean, 55 minutes). The intraoperative blood loss was 50-150 mL (mean, 64 mL). The hospital stay was 5-15 days (mean, 8.7 days). All incisions healed by first intention. Twenty-eight patients were followed up 12-18 months with an average of 13.6 months. The fracture reduction quality was rated as excellent in 9 cases (32.1%), good in 17 cases (60.7%), and poor in 2 cases (7.1%) by Chang's criteria. Parker-Palmer score was 6-9 (mean, 7.9) at last follow-up.

Conclusion: High plane intertrochanteric femur fracture is a special type of intertrochanteric fracture, which can be diagnosed by imaging examination. PFNA fixation can achieve satisfactory results and prevent the occurrence of internal fixation failure effectively.
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http://dx.doi.org/10.7507/1002-1892.202007114DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8171748PMC
March 2021

Comprehensive analysis of coding sequence architecture features and gene expression in .

Physiol Mol Biol Plants 2021 Feb 18;27(2):213-222. Epub 2021 Feb 18.

Grassland Agri-Husbandry Research Center, College of Grassland Science, Qingdao Agricultural University, Qingdao, China.

Coding sequence (CDS) architecture affects gene expression levels in organisms. Codon optimization can increase the gene expression level. Therefore, understanding codon usage patterns has important implications for research on genetic engineering and exogenous gene expression. To date, the codon usage patterns of many model plants have been analyzed. However, the relationship between CDS architecture and gene expression in remains poorly understood. According to the results of genome sequencing, has many resistant genes that can be used to improve the cultivated peanut. In this study, bioinformatic approaches were used to estimate CDS architectures, including frequency of the optimal codon (Fop), polypeptide length and GC contents at the first (GC1), second (GC2) and third (GC3) codon positions. In addition, RNA-seq datasets were downloaded from PeanutBase. The relationships between gene expression and CDS architecture were assessed both under normal growth as well as nematode and drought stress conditions. A total of 26 codons with high frequency were identified, which preferentially ended with A or T in CDSs under the above-mentioned three conditions. A similar CDS architecture was found in differentially expressed genes (DEGs) under nematode and drought stresses. The GC1 content differed between DEGs and non-differentially expressed genes (NDEGs) under both drought and nematode stresses. The expression levels of DEGs were affected by different CDS architectures compared with NDEGs under drought stress. In addition, no correlation was found between differential gene expression and CDS architecture neither under nematode nor under drought stress. These results aid the understanding of gene expression in .
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http://dx.doi.org/10.1007/s12298-021-00938-yDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7907404PMC
February 2021

Emission characteristics and ozone formation potentials of gaseous pollutants from in-use methanol-, CNG- and gasoline-fueled vehicles.

Environ Monit Assess 2021 Mar 6;193(4):164. Epub 2021 Mar 6.

School of Water and Environment, Chang'an University, Xi'an, 710064, China.

Alternative-fueled vehicles have been introduced to solve the problem of the energy crisis and address air pollution. However, typical pollutants (e.g., methane and methanol) are emitted through combustion of the alternative fuel. In this study, the concentrations of regulated pollutants (CO, NO) and unregulated pollutants (CH, methanol, formaldehyde, and 8 NMHC species) in the exhaust from methanol, CNG, and gasoline-fueled vehicles (MV, NGV, and GV) were measured systematically on a chassis dynamometer during an in-use vehicle driving cycle. The emission factors of these gaseous pollutants were calculated, and the ozone formation potential (OFP) of each ozone precursor measured in this work was evaluated with the MIR scale. The results showed that NO and NMHC species exhausted from the MV and NGV decreased significantly than that from the GV. However, the unburned pollutants exhausted from MV and NGV warrant attention. For the OFPs, CO was the largest contributor for all tested vehicles. Formaldehyde was ranked the second for the MV and NGV. Among the tested vehicles, the OFPs of NGV were the lowest. The results are helpful in quantitating analysis of the vehicle emissions and evaluating the impacts of alternative-fueled vehicles on atmospheric environment.
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http://dx.doi.org/10.1007/s10661-021-08951-0DOI Listing
March 2021

RNF39 mediates K48-linked ubiquitination of DDX3X and inhibits RLR-dependent antiviral immunity.

Sci Adv 2021 Mar 5;7(10). Epub 2021 Mar 5.

Department of Immunology and Key Laboratory for Experimental Teratology of the Chinese Ministry of Education, School of Basic Medical Science, Shandong University, Jinan, Shandong, China.

Retinoic acid-inducible gene-I (RIG-I)-like receptors (RLRs) are major cytosolic RNA sensors and play crucial roles in initiating antiviral innate immunity. Furthermore, RLRs have been implicated in multiple autoimmune disorders. Thus, RLR activation should be tightly controlled to avoid detrimental effects. "DEAD-box RNA helicase 3, X-linked" (DDX3X) is a key adaptor in RLR signaling, but its regulatory mechanisms remain unknown. Here, we show that the E3 ubiquitin ligase RNF39 inhibits RLR pathways through mediating K48-linked ubiquitination and proteasomal degradation of DDX3X. Concordantly, deficiency enhances RNA virus-triggered innate immune responses and attenuates viral replication. Thus, our results uncover a previously unknown mechanism for the control of DDX3X activity and suggest RNF39 as a priming intervention target for diseases caused by aberrant RLR activation.
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http://dx.doi.org/10.1126/sciadv.abe5877DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7935364PMC
March 2021

IL-17B/IL-17RB signaling cascade contributes to self-renewal and tumorigenesis of cancer stem cells by regulating Beclin-1 ubiquitination.

Oncogene 2021 Mar 1;40(12):2200-2216. Epub 2021 Mar 1.

Department of Laboratory Medicine, Affiliated Hospital of Jining Medical University, Jining Medical University, Jining, Shandong, PR China.

Cancer stem cells (CSCs) are characterized by robust self-renewal and tumorigenesis and are responsible for metastasis, drug resistance, and angiogenesis. However, the molecular mechanisms for the regulation of CSC homeostasis are incompletely understood. This study demonstrated that the interleukin-17 (IL-17)B/IL-17RB signaling cascade promotes the self-renewal and tumorigenesis of CSCs by inducing Beclin-1 ubiquitination. We found that IL-17RB expression was significantly upregulated in spheroid cells and Lgr5-positive cells from the same tumor tissues of patients with gastric cancer (GC), which was closely correlated with the degree of cancer cell differentiation. Recombinant IL-17B (rIL-17B) promoted the sphere-formation ability of CSCs in vitro and enhanced tumor growth and metastasis in vivo. Interestingly, IL-17B induced autophagosome formation and cleavage-mediated transformation of LC3 in CSCs and 293T cells. Furthermore, inhibition of autophagy activation by ATG7 knockdown reversed rIL-17B-induced self-renewal of GC cells. In addition, we showed that IL-17B also promoted K63-mediated ubiquitination of Beclin-1 by mediating the binding of tumor necrosis factor receptor-associated factor 6 to Beclin-1. Silencing IL-17RB expression abrogated the effects of IL-17B on Beclin-1 ubiquitination and autophagy activation in GC cells. Finally, we showed that IL-17B level in the serum of GC patients was positively correlated with IL-17RB expression in GC tissues, and IL-17B could induce IL-17RB expression in GC cells. Overall, the results elucidate the novel functions of IL-17B for CSCs and suggest that the intervention of the IL-17B/IL-17RB signaling pathway may provide new therapeutic targets for the treatment of cancer.
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http://dx.doi.org/10.1038/s41388-021-01699-4DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7994204PMC
March 2021

MiR-29a Alleviates High Glucose-induced Inflammation and Mitochondrial Dysfunction via Modulation of IL-6/STAT3 in Diabetic Cataracts.

Curr Eye Res 2021 Feb 21:1-8. Epub 2021 Feb 21.

Tianjin Eye Hospital, Tianjin Key Lab of Ophthalmology and Visual Science, Tianjin Eye Institute, Clinical College of Ophthalmology Tianjin Medical University, Tianjin, China.

: This study was designed to reveal the role of miR-29a in high glucose-induced cellular injury through the modulation of IL-6/STAT3 in diabetic cataracts.: The expression of miR-29a and STAT3 in the lens capsules of patients with or without diabetes was determined by RT-PCR. The levels of the IL-6 proinflammatory cytokine in the aqueous humor were detected by ELISA. HLE B-3 cells were cultured in normal glucose (NG; 5 mM) or high glucose (HG; 40 mM). After transfection with miR-29a, si-STAT3, or a negative control vector, the levels of IL-6 and STAT3 were detected. A CCK-8 assay was used to determine cell viability. We used flow cytometry to assess changes in reactive oxygen species (ROS) production, mitochondrial membrane potential (MMP), and apoptosis induced by oxidative stress. Western blotting was used to determine the expression of the oxidative injury markers superoxide dismutase (SOD) and malondialdehyde (MDA) and the apoptosis markers Bcl-2 and Bax.: Reduced miR-29a, increased STAT3 expression, and IL-6 release were demonstrated in the lens capsules and aqueous humor of patients with diabetes. The stimulation of apoptosis and the loss of MMP induced by HG were attenuated by transfection with a miR-29a mimic and si-STAT3. ROS production, increased MDA content, decreased SOD activity, and upregulation of the apoptotic proteins Bcl-2/Bax were also partially alleviated by miR-29a overexpression, which shows their roles in oxidative injury. Furthermore, transfection with a STAT3 overexpression vector reversed the effects of miR-29a.: In conclusion, miR-29a mitigated HG-induced oxidative injury and exerted protective effects via IL-6/STAT3 signaling. Thus, miR-29a may be a potential therapeutic agent for diabetic cataracts.
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http://dx.doi.org/10.1080/02713683.2021.1887272DOI Listing
February 2021

Oxocrebanine: A Novel Dual Topoisomerase inhibitor, Suppressed the Proliferation of Breast Cancer Cells MCF-7 by Inducing DNA Damage and Mitotic Arrest.

Phytomedicine 2021 Apr 10;84:153504. Epub 2021 Feb 10.

Key Laboratory of Tropical Translational Medicine of Ministry of Education, Hainan Key Laboratory for Research and Development of Tropical Herbs, School of Pharmacy, Hainan Medical University, Haikou, 57199, China. Electronic address:

Background: DNA topoisomerase (Topo) inhibition plays key role in breast cancer treatment. Stephania hainanensis H. S. Lo et Y. Tsoong (S. hainanensis), a Li nationality plant that has abundant aporphine alkaloids, can inhibit Topo.

Purpose: To identify a dual Topo inhibitor, a deep and systematic study of active aporphine alkaloids in S. hainanensis and their mechanisms of inhibiting breast cancer proliferation and Topo activity are essential.

Study Design: This study aimed to assess the anti-breast cancer and Topo inhibitory activities of oxocrebanine and explore the underlying mechanisms.

Methods: The growth inhibitory activities of 12 compounds in S. hainanensis were screened by MTT assay in MCF-7, SGC-7901, HepG-2 cells, and compared with the effects on human normal mammary epithelial MCF-10A cells as non cancer control cells. The Topo inhibitory activity was assessed by DNA relaxation and unwinding assays, kDNA decatenation assay and western blot. Cell cycle and autophagy analyses were carried out with flow cytometry and staining. Acridine orange staining and α-tubulin morphology were observed by fluorescence microscopy. Western blot was used to examine microtubule assembly dynamics and the expression levels of key proteins associated with DNA damage, autophagy and mitotic arrest.

Results: Oxocrebanine was the anti-breast cancer active alkaloid in S. hainanensis. It exhibited the best inhibitory effect on MCF-7 cells with an IC of 16.66 μmol/l, and had only weak effect on the proliferation of MCF-10A cells. Oxocrebanine inhibited Topo I and II α in a cell-free system and in MCF-7 cells. The DNA unwinding assay suggested that oxocrebanine intercalated with DNA as a catalytic inhibitor. Oxocrebanine regulated the levels of Topo I and IIα and DNA damage-related proteins. Oxocrebanine led to the mitotic arrest, and these effects occurred through both p53-dependent and p53-independent pathways. Oxocrebanine induced autophagy, abnormal α-tubulin morphology and stimulated enhanced microtubule dynamics.

Conclusion: Oxocrebanine was the anti-breast cancer active aporphine alkaloid in S. hainanensis. Oxocrebanine was a Topo I/IIα dual inhibitor, catalytic inhibitor and DNA intercalator. Oxocrebanine caused DNA damage, autophagy, and mitotic arrest in MCF-7 cells. Oxocrebanine also disrupted tubulin polymerization. Accordingly, oxocrebanine held a great potential for development as a novel dual Topo inhibitor for effective breast cancer treatment.
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http://dx.doi.org/10.1016/j.phymed.2021.153504DOI Listing
April 2021

miR-29b restrains cholangiocarcinoma progression by relieving DNMT3B-mediated repression of CDKN2B expression.

Aging (Albany NY) 2021 02 17;13(4):6055-6065. Epub 2021 Feb 17.

Department of Hepatobiliary Surgery, The Hospital Affiliated to Guizhou Medical University, Guiyang, Guizhou, P. R. of China.

Numerous studies have reported the important role of microRNAs (miRNAs) in human cancers. Although abnormal miR-29b expression has been linked to tumorigenesis in several cancers, its role in cholangiocarcinoma remains largely unknown. We found that miR-29b expression is frequently downregulated in human cholangiocarcinoma QBC939 cells and in clinical tumor samples. In cholangiocarcinoma patients, low miR-29b expression predicts poor overall survival. Overexpression of miR-29b in QBC939 cells inhibited proliferation, induced G1 phase cycle arrest, and promoted apoptosis. Methylation-specific PCR (MSP) analysis revealed a decreased methylation imprint at the promoter of the cell cycle inhibitor gene CDKN2B in cells overexpressing miR-29b. After identifying the DNA methyltransferase DNMT3B as a putative miR-29b target, luciferase reporter assays confirmed a suppressive effect of miR-29b on DNMT3B expression. Accordingly, we detected an inverse correlation between miR-29b and DNMT3B expression in clinical cholangiocarcinoma specimens. In QBC939 cells, DNMT3B overexpression promoted proliferation and inhibited apoptosis. DNMT3B silencing, in turn, led to increased CDKN2B expression. We also observed significant growth arrest in subcutaneous tumors formed in nude mice by QBC939 cells overexpressing miR-29b. These findings suggest miR-29b functions as a tumor suppressor in cholangiocarcinoma by relieving DNMT3B-mediated repression of CDKN2B expression.
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http://dx.doi.org/10.18632/aging.202549DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7950249PMC
February 2021

Ambient sunlight-driven photothermal methanol dehydrogenation for syngas production with 32.9 % solar-to-hydrogen conversion efficiency.

iScience 2021 Feb 9;24(2):102056. Epub 2021 Jan 9.

International Center for Materials Nanoarchitectonics (WPI-MANA), National Institute for Materials Science (NIMS), 1-1 Namiki, Tsukuba, Ibaraki 305-0044, Japan.

Methanol dehydrogenation is an efficient way to produce syngas with high quality. The current efficiency of sunlight-driven methanol dehydrogenation is poor, which is limited by the lack of excellent catalysts and effective methods to convert sunlight into chemicals. Here, we show that atomically substitutional Pt-doped in CeO nanosheets (Pt-CeO) exhibit excellent methanol dehydrogenation activity with 500-hr level catalytic stability, 11 times higher than that of Pt nanoparticles/CeO. Further, we introduce a photothermal conversion device to heat Pt-CeO up to 299°C under 1 sun irradiation owning to efficient full sunlight absorption and low heat dissipation, thus achieving an extraordinarily high methanol dehydrogenation performance with a 481.1 mmol g h of H production rate and a high solar-to-hydrogen (STH) efficiency of 32.9%. Our method represents another progress for ambient sunlight-driven stable and active methanol dehydrogenation technology.
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http://dx.doi.org/10.1016/j.isci.2021.102056DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7841357PMC
February 2021

Efficacy and safety assessment of severe COVID-19 patients with Chinese medicine: A retrospective case series study at early stage of the COVID-19 epidemic in Wuhan, China.

J Ethnopharmacol 2021 Sep 30;277:113888. Epub 2021 Jan 30.

China Academy of Chinese Medical Sciences, Beijing, China. Electronic address:

Ethnopharmacological Relevance: The coronavirus disease 2019 (COVID-19) has formed a global pandemic since late 2019. Benefitting from the application experience of Chinese Medicine (CM) for influenza and SARS, CM has been used to save patients at the early stage of COVID-19 outbreak in China.

Aim Of The Study: In order to evaluate the efficacy and safety of CM, and compare with Western Medicine (WM) for COVID-19, we conducted a retrospective case series study based on the patients in Wuhan Jinyintan Hospital, Wuhan, China.

Methods: The inclusion and exclusion criteria of data extraction were set for this retrospective study. All patients who were admitted by the Wuhan Jinyintan Hospital between January 17th and February 25th 2020 were considered. In addition, patients enrolled met the severe defined by the guidelines released by the National Health Commission of the People's Republic of China. In these cases included in the study, CM or WM treatment was selected according to the wishes of the patients at the beginning of hospitalization. The patients in CM group were treated with Huashi Baidu granule (137 g po, bid) combined with the injections of Xiyanping (100 mg iv, bid), Xuebijing (100 ml iv, bid) and Shenmai (60 ml iv, qd) according to the syndrome of epidemic toxin blocking the lung in the theory of Traditional Chinese Medicine. The WM group received antiviral therapy (including abidor capsule 0.2 g po, tid; Lopinavir-Ritonavir tablets, 500 mg po, bid), antibiotics (such as cefoperazone 2 g iv, bid; moxifloxacin hydrochloride tablets, 0.4 g po, qd) or corticosteroid therapy (such as methylprednisolone succinate sodium 40 mg iv, qd; prednisone, 30 mg po, qd). In addition, patients in both groups received routine supportive treatment, including oxygen inhalation, symptomatic therapy, and/or human intravenous immunoglobulin, and/or serum albumin, and treatment for underlying diseases. The clinical outcomes were evaluated based on changes related with clinical manifestations, computer tomography (CT) scan images, and laboratory examinations before and after the treatment.

Results: 55 severe COVID-19 patients, with 23 in CM group and 32 in WM group, were included for analyzed. There was no case of death, being transferred to ICU, or receiving invasive mechanical ventilation in two groups during hospitalization. The median time of SARS-CoV-2 RNA clearance in CM and WM group were 12 days and 15.5 days respectively, the ratio of nucleic acid negative conversion of CM group at different follow-up time points was significantly higher than that of WM group (HR: 2.281, P = 0.018). Further, the chest CT imaging showed more widely lung lesion opacity absorbed in the CM group. The high sensitivity C-reactive protein and serum ferritin decreased significantly in the CM group (P<0.05). There was no significant difference in adverse events in terms of liver function and renal function between the two groups.

Conclusion: Based on this retrospective analysis from Wuhan Jinyintan Hospital, CM has better effects in SARS-CoV-2 RNA clearance, promoting lung lesion opacity absorbed and reducing inflammation in severe COVID-19 patients, which is effective and safe therapy for treating severe COVID-19 and reducing mortality.
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http://dx.doi.org/10.1016/j.jep.2021.113888DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7847283PMC
September 2021

Joint optic disc and cup segmentation based on densely connected depthwise separable convolution deep network.

BMC Med Imaging 2021 01 28;21(1):14. Epub 2021 Jan 28.

South China Normal University, Guangzhou, 510006, China.

Background: Glaucoma is an eye disease that causes vision loss and even blindness. The cup to disc ratio (CDR) is an important indicator for glaucoma screening and diagnosis. Accurate segmentation for the optic disc and cup helps obtain CDR. Although many deep learning-based methods have been proposed to segment the disc and cup for fundus image, achieving highly accurate segmentation performance is still a great challenge due to the heavy overlap between the optic disc and cup.

Methods: In this paper, we propose a two-stage method where the optic disc is firstly located and then the optic disc and cup are segmented jointly according to the interesting areas. Also, we consider the joint optic disc and cup segmentation task as a multi-category semantic segmentation task for which a deep learning-based model named DDSC-Net (densely connected depthwise separable convolution network) is proposed. Specifically, we employ depthwise separable convolutional layer and image pyramid input to form a deeper and wider network to improve segmentation performance. Finally, we evaluate our method on two publicly available datasets, Drishti-GS and REFUGE dataset.

Results: The experiment results show that the proposed method outperforms state-of-the-art methods, such as pOSAL, GL-Net, M-Net and Stack-U-Net in terms of disc coefficients, with the scores of 0.9780 (optic disc) and 0.9123 (optic cup) on the DRISHTI-GS dataset, and the scores of 0.9601 (optic disc) and 0.8903 (optic cup) on the REFUGE dataset. Particularly, in the more challenging optic cup segmentation task, our method outperforms GL-Net by 0.7[Formula: see text] in terms of disc coefficients on the Drishti-GS dataset and outperforms pOSAL by 0.79[Formula: see text] on the REFUGE dataset, respectively.

Conclusions: The promising segmentation performances reveal that our method has the potential in assisting the screening and diagnosis of glaucoma.
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http://dx.doi.org/10.1186/s12880-020-00528-6DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7842021PMC
January 2021

Environmental management strategy in response to COVID-19 in China: Based on text mining of government open information.

Sci Total Environ 2021 May 15;769:145158. Epub 2021 Jan 15.

School of Environmental Science and Engineering, Shandong University, Qingdao, Shandong 266237, China.

Coronavirus disease 2019 (COVID-19) is a global pandemic and a major health emergency. In the process of fighting against COVID-19, the China Ministry of Ecology and Environment (MEE) responded quickly and set up a working group as soon as possible. This article uses text mining to retrospectively analyze the government's public information on the website of MEE during the epidemic, sort out the timeline of MEE's response to COVID-19. We find that MEE's work during the COVID-19 pandemic is focused on medical waste and wastewater treatment, environment emergency monitoring, pollution prevention, and other environmental management for supporting economic recovery. It drafted three main medical waste management plans, an emergency environmental monitoring plan, and formulated "two lists" - a Positive checklist for Environmental impact assessment (EIA) approval and a positive checklist for supervision and enforcement, to promote the resumption of work and production. 2020 is the final year of China's "three years of fighting pollution prevention and control". In the case of the sudden COVID-19 epidemic, the Chinese environment department has ensured that the quality of the ecological environment has not been affected by the epidemic prevention and control while ensuring the smooth progress of the fight against pollution. China's medical waste disposal capacity has also been greatly improved during this epidemic. The review of China's environmental management strategy in response to COVID-19 can provide a reference for countries in the world that are still in the critical period of epidemic control; it can provide action guidelines for the ecological environment system to respond to sudden pandemic events in the future.
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http://dx.doi.org/10.1016/j.scitotenv.2021.145158DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7837070PMC
May 2021

CRISPR/Cas9-mediated grna gene knockout leads to neurodevelopmental defects and motor behavior changes in zebrafish.

J Neurochem 2021 05 15;157(3):520-531. Epub 2021 Feb 15.

Laboratory of Gene Therapy, Department of Biochemistry, College of Life Sciences, Shaanxi Normal University, Xi'an, Shaanxi, P.R. China.

Progranulin (PGRN) is a secreted glycoprotein with multiple biological functions in early embryogenesis, anti-inflammation, and neurodegeneration. A good model for the functional study of PGRN is the zebrafish with knockdown or knockout of grn, the gene encoding PGRN. Morpholino oligonucleotides (MOs) and zinc finger nucleases have been used to generate zebrafish grn models, yet they have shown inconsistent phenotypes due to either the neurotoxicity of the MOs or possible genetic compensation responses during gene editing. In this study, we generated stable grna (one of the major grn homologues of zebrafish) knockout zebrafish by using CRISPR/Cas9-mediated genome editing. A grna sgRNA was designed to target the similar repeated sequence shared by exon 13, exon 15, and exon 19 in zebrafish. The F1 generation with the frameshift mutation of + 4 bp (the addition of 4 bp to exon15), which causes a premature termination, was obtained and subjected to morphological and behavioral evaluation. The grna knockout zebrafish showed neurodevelopmental defects, including spinal motor neurons with shorter axons, decreased sensory hair cells, thinning of the outer nuclear layer and thickening of the inner nuclear layer of the retina, decreased expression of rhodopsin in the cone cells, and motor behavior changes. Moreover, the phenotypes of grna knockout zebrafish could be rescued with the Tol2 system carrying the grna gene. The grna knockout zebrafish model generated in this study provides a useful tool to study PGRN function and has potential for high-throughput drug screening for disease therapy.
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http://dx.doi.org/10.1111/jnc.15307DOI Listing
May 2021

Evaluation of a New IOL Power Calculator in Cataract Patients with Normal and Long Axial Lengths.

Semin Ophthalmol 2020 Nov 17;35(7-8):370-376. Epub 2021 Jan 17.

Cataract Department, Clinical College of Ophthalmology, Tianjin Medical University , Tianjin, China.

: To evaluate the accuracy of Ophtha Top and consistency between Ophtha Top and IOLMaster 500 in intraocular lens refractive power calculation among cataract patients with normal and long axial lengths. : This study included cataract patients scheduled for phacoemulsification and IOL implantation surgery. The IOL power was calculated using Ophtha Top and IOLMaster 500 (integrated with SRK/T, Hoffer Q, Holladay 1 formula). The accuracy of IOL power calculation between Ophtha Top and IOLMaster 500 was compared. Bland-Altman plots were also used to assess agreement between Ophtha Top and IOLMaster 500. : Ninety-four patients (94 eyes) were included. The mean values of the arithmetic and absolute prediction errors of Ophtha Top were -0.22 ± 0.62 D and 0.52 ± 0.40 D for whole sample. Absolute refractive error showed no significant difference between Ophtha Top and IOLMaster 500 using 3 traditional formulas in eyes with normal and long axial lengths. In normal eyes, mean and medium absolute error of Ophtha Top was 0.49D and 0.48D, which were comparable to that of IOLMaster 500 (Hoffer Q:0.47D; 0.40D & Holladay 1: 0.48D; 0.37D). Similar trend was found in long eyes (Ophtha Top:0.58 D & IOLMaster using SRK/T:0.53D). : Ophtha Top based on real ray-tracing method could provide predictable outcomes in all eyes, which was comparable to outcomes from IOLMaster 500 using SRK/T, Hoffer Q, Hollday 1 formula. Ophtha Top would be a promising alternative choice for IOL power calculation.
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http://dx.doi.org/10.1080/08820538.2020.1871033DOI Listing
November 2020