Publications by authors named "Hui Kong"

224 Publications

The structure of blood-tumor barrier and distribution of chemotherapeutic drugs in non-small cell lung cancer brain metastases.

Cancer Cell Int 2021 Oct 24;21(1):556. Epub 2021 Oct 24.

Department of Respiratory and Critical Care Medicine, The First Affiliated Hospital of Nanjing Medical University, Nanjing, 210029, Jiangsu, People's Republic of China.

Background: Brain metastasis is an important cause of increased mortality in patients with non-small cell lung cancer (NSCLC). In brain metastasis, the blood-brain barrier (BBB) is frequently impaired, forming blood-tumor barrier (BTB). The efficacy of chemotherapy is usually very poor. However, the characteristics of BTB and the impacts of BTB on chemotherapeutic drug delivery remain unclear. The present study investigated the structure of BTB, as well as the distribution of routine clinical chemotherapeutic drugs in both brain and peripheral tumors.

Methods: Bioluminescent image was used to monitor the tumor load after intracranial injection of lung cancer Lewis cells in mice. The permeability of BBB and BTB was measured by fluorescent tracers of evans blue and fluorescein sodium. Transmission electron microscopy (TEM), immunohistochemistry and immunofluorescence were performed to analyze structural differences between BBB and BTB. The concentrations of chemotherapeutic drugs (gemcitabine, paclitaxel and pemetrexed) in tissues were assayed by liquid chromatography with tandem mass spectrometry (LC-MS/MS).

Results: Brain metastases exhibited increased BTB permeability compared with normal BBB detected by fluorescence tracers. TEM showed abnormal blood vessels, damaged endothelial cells, thick basement membranes, impaired intercellular endothelial tight junctions, as well as increased fenestrae and pinocytotic vesicles in metastatic lesions. Immunohistochemistry and immunofluorescence revealed that astrocytes were distributed surrounded the blood vessels both in normal brain and the tumor border, but no astrocytes were found in the inner metastatic lesions. By LC-MS/MS analysis, gemcitabine showed higher permeability in brain metastases.

Conclusions: Brain metastases of lung cancer disrupted the structure of BBB, and this disruption was heterogeneous. Chemotherapeutic drugs can cross the BTB of brain metastases of lung cancer but have difficulty crossing the normal BBB. Among the three commonly used chemotherapy drugs, gemcitabine has the highest distribution in brain metastases. The permeability of chemotherapeutic agents is related to their molecular weight and liposolubility.
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http://dx.doi.org/10.1186/s12935-021-02263-6DOI Listing
October 2021

Assembly of a Hexameric Cluster of Polyoxomolybdotriphosphonate Builts from [Zn(HO){TeMoO}{N(CHPO)}] Subunits and Its Optical and Catalytic Properties.

Inorg Chem 2021 Oct 4;60(20):15759-15767. Epub 2021 Oct 4.

Henan Key Laboratory of Polyoxometalate Chemistry, College of Chemistry and Chemical Engineering, Henan University, Kaifeng, Henan 475004, People's Republic of China.

The crown-shaped organotriphosphonate-modified 36-molybdenum cluster (NH)NaH[Zn(HO)TeMoO{N(CHPO)}]·23HO () has been synthesized, which is the largest zinc-containing organophosphonate-based polyoxometalate to date. Compound was prepared in buffer solution (pH 5.5) with heptamolybdate and amino trimethylene phosphonic acid (ATMP) as the organic ligand. The polyanion constructed from a hexmeric assembly of [Zn(HO){TeMoO}{N(CHPO)}] subunits has been fully investigated by a few characterization methods. In this work, we discovered that exhibited reversible photochromism and it changed from white to reddish brown upon UV irradiation. In addition, compound , as a catalyst, can oxidize sulfides to sulfoxides, showing a high yield/conversion and a good selectivity.
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http://dx.doi.org/10.1021/acs.inorgchem.1c02417DOI Listing
October 2021

Organophosphonate-Functionalized Telluromolybdate Containing a [TeMoO] Building Block and Its Catalytic Efficiency for Knoevenagel Condensation.

Inorg Chem 2021 Oct 17;60(19):14872-14879. Epub 2021 Sep 17.

Henan Key Laboratory of Polyoxometalate Chemistry, College of Chemistry and Chemical Engineering, Henan University, Kaifeng, Henan 475004, China.

A novel organodiphosphonate-based telluromolybdate cluster, (NH)NaH[TeMoO(CoMoOL)]·11HO [; L = (OP)C(O)(CH)NH], has been successfully synthesized by a simple one-pot aqueous reaction. Intriguingly, the [TeMoO] subunit with tetrahedral geometry of TeO is observed in the organophosphonate-functionalized polyoxometalates for the first time. Compound was prepared in a buffer solution (pH = 5.5) with alendronic acid (Ale) and (NH)MoO·4HO as raw materials. The polyanion [TeMoO(CoMoOL)] was constructed from four {MoOL} subunits encapsulating an interesting Te-Mo heterometal subunit [TeMoO] through four CoO octahedra and has been fully characterized by routine techniques. In addition, compound , as a heterogeneous catalyst, shows good conversion (92%) and high selectivity (99%) for Knoevenagel condensation reaction.
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http://dx.doi.org/10.1021/acs.inorgchem.1c02235DOI Listing
October 2021

The neuroprotective effect of pretreatment with carbon dots from Crinis Carbonisatus (carbonized human hair) against cerebral ischemia reperfusion injury.

J Nanobiotechnology 2021 Aug 28;19(1):257. Epub 2021 Aug 28.

Center of Scientific Experiment, Beijing University of Chinese Medicine, 11 Beisanhuandong Road, Chaoyang District, Beijing, 100029, China.

Background: Cerebral infarction and cerebral hemorrhage, also known as "stroke", is one of the leading cause of death. At present, there is no real specific medicine for stroke. Crinis Carbonisatus (named Xue-yu-tan in Chinese), produced from carbonized hair of healthy human, and has been widely applied to relieve pain and treat epilepsy, stroke and other diseases in China for thousands of years.

Results: In this work, a new species of carbon dots derived from Crinis Carbonisatus (CrCi-CDs) were separated and identified. And the neuroprotective effect of carbon dots from CrCi were evaluated using the middle cerebral artery occlusion (MCAO) model. Neurological deficit score and infarction volume was assessed, evans blue content of ischemic hemispheres was measured, the concentrations of inflammatory factors, tumor necrosis factor-α (TNF-α), interleukin-6 (IL-6) in the cortex were measured, and the levels of neurotransmitters in the brain were determined. Preconditioning of CrCi-CDs significantly reduced ischemic lesion volume and blood-brain-barrier (BBB) permeability, improved neurologic deficits, decreased the level of TNF-α and IL-6 in MCAO rats, inhibited excitatory neurotransmitters aspartate (Asp) and glutamate (Glu), and increased the level of 5-hydroxytryptamine (5-HT). The RNA-Sequencing results reveal that further potential mechanisms behind the activities may be related to the anti-inflammation effects and inhibition of neuroexcitatory toxicity.

Conclusion: CrCi-CDs performs neuroprotective effect on cerebral ischemia and reperfusion injury, and the mechanisms may correlate with its anti-inflammatory action, which suggested that CrCi-CDs have potential value in clinical therapy on the acute apoplexy cases in combination with thrombolytic drugs.
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http://dx.doi.org/10.1186/s12951-021-00908-2DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8399708PMC
August 2021

Upregulation of thioredoxin contributes to inhibiting diabetic hearing impairment.

Diabetes Res Clin Pract 2021 Sep 25;179:109025. Epub 2021 Aug 25.

Department of Otorhinolaryngology of the Second Hospital, Dalian Medical University, Dalian 116023, LiaoNing Province, China. Electronic address:

Aims: Hair cell reduction was related to diabetes-induced hearing loss. Oxidative stress, endoplasmic reticulum stress, and autophagy participate in this process. Thioredoxin (Trx) is a protein with many biological functions which can regulate them. In this study, aiming to clarify protective effect of Trx on diabetic hearing loss and to identify an early potential therapeutic target for diabetic hearing impairment in the future.

Methods: Trx transgenic (Tg) mice were used to establish a diabetic model by intraperitoneally injecting streptozotocin (STZ) and with/without SF or PX12 treatment. Succinate dehydrogenase (SDH) staining was used to evaluate the loss of hair cells. The relative expression of related proteins and genes was detected using western blotting and qRT-PCR.

Results: In vivo, loss of outer hair cells was observed. However, it can be delayed Trx overexpression. Moreover, the expression of PGC-1α, bcl-2 and LC3 was increased in Tg(+)-DM mice compared with Tg(-)-DM mice. The expression of ASK1, Txnip, GRP78, CHOP and p62 was decreased in Tg(+)-DM mice compared with Tg(-)-DM mice.

Conclusions: Upregulation of Trx protects diabetes-induced cochlear hair cells reduction. The underlying mechanisms were related to the regulation of ER stress through ASK1 and the mitochondrial pathway or autophagy via Txnip.
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http://dx.doi.org/10.1016/j.diabres.2021.109025DOI Listing
September 2021

Risk factors for carbapenem-resistant infection and associated clinical outcomes.

Am J Transl Res 2021 15;13(6):7276-7281. Epub 2021 Jun 15.

Department of Respiratory Medicine, The First Affiliated Hospital of Nanjing Medical University Nanjing 210029, Jiangsu, China.

Objective: To evaluate the risk factors and clinical outcomes of carbapenem-resistant () (CRKP) infection.

Materials And Methods: A case-control study was performed from January 2017 to September 2017. The risk factors and clinical outcomes of CRKP cases (n = 91) were compared with those of the controls infected with carbapenem-susceptible (CSKP) (n = 91). Antibiotic susceptibility was determined using Etest while the type of bacteria was identified by Vitek 2.

Results: CRKP infection was associated with prior use of carbapenems, β-lactam antibiotics, tigecycline, and hormones; complications with cerebrovascular lesions; chronic obstructive pulmonary disease; as well as prolonged hospitalization. Multivariable analysis showed that the use of carbapenem independently correlates with carbapenem resistance in the multivariable analysis. Carbapenem resistance, mechanical ventilation, tracheotomy, deep vein cannulation, indwelling urinary tract catheter, ICU treatment, and high Acute Physiology, Age, Chronic Health Evaluation II (APACHE II) scores were related to in-hospital mortality.

Conclusion: CRKP is a widely spread pathogen associated with high in-hospital mortality. Minimizing the use of antimicrobials, specifically the carbapenems, may be effective to reduce CRKP infection.
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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8290679PMC
June 2021

Gastroprotective effects of derived carbon dots on ethanol-induced gastric ulcers in rats.

Nanomedicine (Lond) 2021 08 15;16(19):1657-1671. Epub 2021 Jul 15.

School of Basic Medical Sciences, Beijing University of Chinese Medicine, Beijing, 100029, China.

To evaluate the gastroprotective effects of carbon dots (NRN-CDs) on ethanol-induced gastric ulcers in rats. NRN-CDs synthesized and characterized by transmission electron microscopy, ultraviolet, fluorescence and Fourier transform infrared spectroscopy, x-ray photoelectron spectroscopy, x-ray diffraction and zeta potential analyzer. Their gastroprotective effects toward ethanol-induced gastric ulcers were evaluated in male Sprague-Dawley rats. NRN-CDs showed an average diameter of 2.33 ± 0.42 nm and a lattice spacing of 0.29 nm. Pretreatment with NRN-CDs significantly decreased the ulcer index and attenuated the severity of gastric mucosal damage, indicating that NRN-CDs exerted potent gastric protective effect. Moreover, the gastroprotection effect was related to the regulation of oxidative stress and inflammatory factors. NRN-CDs could be developed as a potential drug for the treatment of gastric ulcers.
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http://dx.doi.org/10.2217/nnm-2020-0472DOI Listing
August 2021

Abnormal Coagulation Function of Patients With COVID-19 Is Significantly Related to Hypocalcemia and Severe Inflammation.

Front Med (Lausanne) 2021 16;8:638194. Epub 2021 Jun 16.

Department of Respiratory Medicine, The First Affiliated Hospital of Nanjing Medical University, Nanjing, China.

This study aimed to detect, analyze, and correlate the clinical characteristics, blood coagulation functions, blood calcium levels, and inflammatory factors in patients with mild and severe COVID-19 infections. The enrolled COVID-19 infected patients were from Wuhan Jin Yin-tan Hospital (17 cases, Wuhan, China), Suzhou Infectious Disease Hospital (87 cases, Suzhou, China), and Xuzhou Infectious Disease Hospital (14 cases, Xuzhou, China). After admission, basic information was collected; X-ray and chest CT images were obtained; and data from routine blood tests, liver and kidney function, myocardial enzymes, electrolytes, blood coagulation function, (erythrocyte sedimentation rate) ESR, C-reactive protein (CRP), IL-6, procalcitonin (PCT), calcitonin, and other laboratory tests were obtained. The patients were grouped according to the clinical classification method based on the pneumonia diagnosis and treatment plan for new coronavirus infection (trial version 7) in China. The measurements from mild (56 cases) and severe cases (51 cases) were compared and analyzed. Most COVID-19 patients presented with fever. Chest X-ray and CT images showed multiple patchy and ground glass opacities in the lungs of COVID 19 infected patients, especially in patients with severe cases. Compared with patients with mild infection, patients with severe infection were older ( = 0.023) and had a significant increase in AST and BUN. The levels of CK, LDH, CK-MB, proBNP, and Myo in patients with severe COVID-19 infection were also increased significantly compared to those in patients with mild cases. Patients with severe COVID-19 infections presented coagulation dysfunction and increased D-dimer and fibrin degradation product (FDP) levels. Severe COVID-19 patients had low serum calcium ion (Ca) concentrations and high calcitonin and PCT levels and exhibited serious systemic inflammation. Ca in COVID-19 patients was significantly negatively correlated with PCT, calcitonin, D-dimer, PFDP, ESR, CRP and IL-6. D-dimer in COVID-19 patients was a significantly positively correlated with CRP and IL-6. In conclusion, patients with severe COVID-19 infection presented significant metabolic dysfunction and abnormal blood coagulation, a sharp increase in inflammatory factors and calcitonin and procalcitonin levels, and a significant decrease in Ca. Decreased Ca and coagulation dysfunction in COVID-19 patients were significantly correlated with each other and with inflammatory factors.
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http://dx.doi.org/10.3389/fmed.2021.638194DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8242574PMC
June 2021

Combining Lactate Dehydrogenase and Fibrinogen: Potential Factors to Predict Therapeutic Efficacy and Prognosis of Patients with Small-Cell Lung Cancer.

Cancer Manag Res 2021 31;13:4299-4307. Epub 2021 May 31.

Department of Respiratory and Critical Care Medicine, The First Affiliated Hospital of Nanjing Medical University, Nanjing, 210029, People's Republic of China.

Background: A growing interest exists in identifying reliable and low-cost biomarkers or factors that could predict the therapeutic response, prognosis, recurrence, and survival in small-cell lung cancer (SCLC). This study aimed to investigate the better predictors of chemotherapy efficacy and prognosis in patients with SCLC receiving first-line chemotherapy and radiotherapy.

Materials And Methods: This study retrospectively retrieved the medical records of patients with SCLC treated with first-line platinum-based chemotherapy and radiotherapy from January 2016 to June 2019 in the First Affiliated Hospital of Nanjing Medical University. Plasma biochemical parameters, clinical features, and overall survival (OS) time were collected. The independent effects of plasma parameters on patient survival were assessed by conducting univariate and multivariate Cox regression analyses. The optimal cut-off values of independent risk factors in the ROC curve and Kaplan-Meier survival analysis were determined using MedCalc software.

Results: Statistically significant differences in lactate dehydrogenase (LDH) and fibrinogen (Fbg) were found between the complete remission + partial remission group and the non-responders, which consisted of stable-disease and progressive-disease groups, after first-line chemotherapy. Multivariate Cox regression analysis showed that LDH and Fbg were independent risk factors in predicting PFS (LDH HR: 1.013, 95% CI: 1.002-1.030, = 0.037; Fbg HR: 1.622, 95% CI: 1.094-2.526, = 0.017) and OS (LDH HR: 1.021, 95% CI: 1.008-1.034, = 0.001; Fbg HR: 2.168, 95% CI: 1.324-3.550, = 0.002). The AUC of LDH and Fbg was 0.77 and 0.745, respectively. The cut-off value of LDH and Fbg in predicting OS was 263 U/L and 4.03 g/L. When these two data were combined, the AUC reached 0.832, better than that of LDH and Fbg alone. The objective response rate (ORR) and OS were significantly different among these three different groups according to the addition of the assigned value ( < 0.05).

Conclusion: Combined retreatment serum LDH and Fbg levels may be a better potential biomarker for predicting the clinical efficacy of chemotherapy and the prognosis of individuals with SCLC. Combining these two parameters could improve prediction efficacy.
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http://dx.doi.org/10.2147/CMAR.S300153DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8178611PMC
May 2021

Gonadal antioxidant responses to seawater acidification and hypoxia in the marine mussel Mytilus coruscus.

Environ Sci Pollut Res Int 2021 Oct 26;28(38):53847-53856. Epub 2021 May 26.

International Research Center for Marine Biosciences, Shanghai Ocean University, Ministry of Science and Technology, Shanghai, China.

This study investigated the combined effects of seawater acidification and hypoxia on the antioxidant response in gonads of the thick shell mussel Mytilus coruscus. Mussels were collected along the Shengsi Island, East China Sea, where oxygen and pH fluctuations frequently occur in summer. Mussels were exposed to three pH (8.1, 7.7, and 7.3) and two dissolved oxygen (DO) levels (6 and 2 mg L) for 21 days followed by a 10-day recovery period (pH 8.1 and DO 6 mg L). Gonad surface area (GSA) and activity of superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GPX), glutathione (GSH), glutathione S-transferase (GST), and malondialdehyde (MDA) in gonad were measured at days 21 and 31. Complex and enzyme-specific responses were observed after the 21-day exposure period. Overall, PCA analysis revealed a stronger effect of pH than DO. Integrated biomarker response (IBR) analysis demonstrated that low pH and DO decreased mussel's antioxidant system and increased oxidative damage with potential consequences for gonad development. Mussels exposed to low pH and DO were only partly able to recover a normal enzymatic activity after 10-day recovery period. This suggests that mussels exposed to short-term pH and DO fluctuations event in the field may suffer lasting negative impacts.
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http://dx.doi.org/10.1007/s11356-021-14584-0DOI Listing
October 2021

An increased risk of lung cancer in combined pulmonary fibrosis and emphysema patients with usual interstitial pneumonia compared with patients with idiopathic pulmonary fibrosis alone: a systematic review and meta-analysis.

Ther Adv Respir Dis 2021 Jan-Dec;15:17534666211017050

Department of Respiratory and Critical Care Medicine, The First Affiliated Hospital of Nanjing Medical University, 300 Guangzhou Road, Nanjing, Jiangsu Province 210029, P. R. China.

Background: Lung cancer is an important complication of combined pulmonary fibrosis and emphysema (CPFE). Whether the risk of lung cancer is higher in CPFE patients with usual interstitial pneumonia (UIP) than those with idiopathic pulmonary fibrosis (IPF) alone, remains controversial. We conducted this systematic review and meta-analysis to evaluate the prevalence of lung cancer in CPFE patients with UIP compared with IPF patients.

Methods: We searched the PubMed, Embase, and Cochrane databases for studies that focused on the incidence of lung cancer in CPFE/UIP and IPF groups. We used a fixed-effects model to analyze the odds ratios (ORs) with 95% confidence intervals (CIs) according to data heterogeneity. The cumulative effects based on the publication year and sample size were assessed by cumulative meta-analysis.

Results: A total of nine studies with 933 patients, including 374 CPFE patients with UIP, fulfilled the inclusion criteria. Overall, CPFE patients with UIP have a higher risk of lung cancer than those with IPF alone (OR = 2.69; 95% CI: 1.78-4.05). There were increased risks of lung cancer in CPFE/UIP patients with the presence of emphysema (OR = 2.93; 95% CI: 1.79-4.79) or emphysema in ⩾10% of the lung volume (OR = 2.22; 95% CI: 1.06-4.68).

Conclusions: Our systematic review and meta-analysis indicated a significantly higher prevalence of lung cancer in CPFE patients with UIP than in patients with IPF alone.
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http://dx.doi.org/10.1177/17534666211017050DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8141990PMC
May 2021

Fasudil Dichloroacetate Alleviates SU5416/Hypoxia-Induced Pulmonary Arterial Hypertension by Ameliorating Dysfunction of Pulmonary Arterial Smooth Muscle Cells.

Drug Des Devel Ther 2021 22;15:1653-1666. Epub 2021 Apr 22.

Department of Respiratory and Critical Care Medicine, The First Affiliated Hospital of Nanjing Medical University, Nanjing, Jiangsu, 210029, People's Republic of China.

Background: Pulmonary arterial hypertension (PAH) is an incurable disease that urgently needs therapeutic approaches. Based on the therapeutic effects of fasudil and dichloroacetate (DCA) on PAH, we aimed to explore the effects and potential mechanism of a new salt, fasudil dichloroacetate (FDCA), in a SU5416 plus hypoxia (SuHx)-induced rat model of PAH.

Methods: The rat model of PAH was established by a single subcutaneous injection of SU5416 (20 mg/kg) followed by hypoxia (10% O) exposure for 3 weeks. FDCA (15, 45, or 135 mg/kg i.g. daily) or the positive control, bosentan (100 mg/kg i.g. daily), were administered from the first day after SU5416 injection. After 3-week hypoxia, hemodynamic parameters, and histological changes of the pulmonary arterial vessels and right ventricle (RV) were assessed. Additionally, in vitro, the effects of FDCA (50 μM), compared with equimolar doses of fasudil, DCA, or fasudil+DCA, on the proliferation, migration, and contraction of human pulmonary arterial smooth muscle cell (PASMC) under hypoxia (1% O) were evaluated.

Results: FDCA dose-dependently attenuated SuHx-induced PAH, with significant reductions in RV systolic pressure, pulmonary artery wall thickness, pulmonary vessel muscularization, perivascular fibrosis, as well as RV hypertrophy and fibrosis. In vitro, FDCA inhibited hypoxia-induced PASMC proliferation, migration, and contraction to a greater degree than fasudil or DCA alone by restoring mitochondrial function, reducing intracellular Ca, and inhibiting calcium/calmodulin-dependent kinase (Ca/CaMK) activity as well as Rho-kinase activity.

Conclusion: FDCA ameliorates hypoxia-induced PASMC dysfunction by inhibiting both Ca/CaMK and Rho-kinase signaling pathways, as well as maintaining mitochondrial homeostasis, thus alleviating SuHx-induced PAH.
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http://dx.doi.org/10.2147/DDDT.S297500DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8076841PMC
April 2021

Edible and highly biocompatible nanodots from natural plants for the treatment of stress gastric ulcers.

Nanoscale 2021 Apr 7;13(14):6809-6818. Epub 2021 Apr 7.

School of Life Sciences, Beijing University of Chinese Medicine, Beijing, 100029, China.

The incidence rate of stress ulcers has increased in recent years, with an increase in life pressure, unavoidable trauma and other factors. The therapy of acute stress ulcers has always been an important challenge. Carbon dots (CDs) have been reported to show excellent biological activities, but research on the stress ulcer curative effect of CDs is unprecedented. Here, we prepared a series of semi-carbonized nanodots (SCNDs) from natural plants or herbs as precursors and the as prepared SCNDs were later proved to be effective in the treatment and inhibition of stress gastric ulcers in a rat model. One kind of SCND from edible and medicinal plants, charred Atractylodes macrocephala (SCNDs-1), is demonstrated in detail for its strong anti-stress gastric ulcer effect with inhibition up to 90% and shows extremely high biocompatibility and ultra-low toxicity. These SCNDs lead to the reduction of inflammatory factors and oxidative stress, and the protection of the gastric mucosa. The SCNDs also reduce the excessive neuroendocrine response caused by stress, regulate the energy metabolism and the structure of intestinal flora, improve the damage to the body caused by the stress state, thus alleviating the occurrence of stress-induced gastric ulcers. This work provides new insights into the preparation of carbon nanomaterials from natural plants through a semi-carbonization process and opens new ways to apply bio-active and bio-safe SCNDs in the modern pharmaceutical field.
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http://dx.doi.org/10.1039/d1nr01099aDOI Listing
April 2021

Green Phellodendri Chinensis Cortex-based carbon dots for ameliorating imiquimod-induced psoriasis-like inflammation in mice.

J Nanobiotechnology 2021 Apr 15;19(1):105. Epub 2021 Apr 15.

School of Traditional Chinese Medicine, Beijing University of Chinese Medicine, Beijing, 100029, China.

Background: Carbon dots (CDs) with multifaceted advantages have provided hope for development brand-new nanodrug for treating thorny diseases. This study developed a green and simple calcination method to prepare novel CDs as promising drug for psoriasis treatment. The as-prepared CDs using Phellodendri Chinensis Cortex (PCC) as sole precursor were characterized by a series of methods, mainly including electron microscopy, optical technology and X-ray photoelectron spectroscopy (XPS).

Results: Results displayed that fluorescence (Quantum yield = 5.63%) and nontoxic PCC-based CDs (PCC-CDs) with abundant chemical groups exhibited solubility and tiny sizes at average of (1.93 ± 0.53) nm, which may be beneficial for its inherent biological activity. Moreover, by using the typical imiquimod (IMQ)-induced psoriasis-like skin mouse model, we firstly demonstrated the pronounced anti-psoriasis activity of as-prepared PCC-CDs on ameliorating the appearance, psoriasis area and severity index (PASI) scores as well as histopathological morphology of both back skin tissues and right ears in IMQ-induced mouse. Further potential mechanisms behind the anti-psoriasis activities may be related to suppress M1 polarization and relatively promote M2 polarization of macrophage both in vitro and in vivo.

Conclusion: These results suggested that PCC-CDs have potential to be an anti-psoriasis candidate for clinical applications to treat psoriasis, which not only provided an evidence for further broadening the biological application of CDs, but also provided a potential hope for application nanodrugs to treat thorny diseases.
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http://dx.doi.org/10.1186/s12951-021-00847-yDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8048166PMC
April 2021

Protective Effects of Radix Sophorae Flavescentis Carbonisata-Based Carbon Dots Against Ethanol-Induced Acute Gastric Ulcer in Rats: Anti-Inflammatory and Antioxidant Activities.

Int J Nanomedicine 2021 26;16:2461-2475. Epub 2021 Mar 26.

School of Traditional Chinese Medicine, Beijing University of Chinese Medicine, Beijing, 100029, People's Republic of China.

Aim: To explore the effects of Radix Sophorae Flavescentis carbonisata-based carbon dots (RSFC-CDs) on an ethanol-induced acute gastric ulcer rat model.

Methods: The structure, optical properties, functional groups and elemental composition of RSFC-CDs synthesized by one-step pyrolysis were characterized. The gastric protective effects of RSFC-CDs were evaluated and confirmed by applying a rat model of ethanol-induced acute gastric ulcers. The underlying mechanisms were investigated through the nuclear factor-kappa B (NF-κB) signalling pathway and oxidative stress.

Results: RSFC-CDs with a diameter ranging from 2-3 nm mainly showed gastric protective effects by reducing the levels of NF-κB, tumour necrosis factor-α (TNF-α), interleukin (IL)-6, superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GSH-Px), glutathione (GSH), malondialdehyde (MDA) and inducible nitric oxide synthase (iNOS) to inhibit ethanol-induced inflammation and oxidative stress.

Conclusion: RSFC-CDs have anti-inflammatory and anti-oxidative effects, making them promising for application in ethanol-induced gastric injury.
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http://dx.doi.org/10.2147/IJN.S289515DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8009542PMC
April 2021

Novel Carbon Dots Derived from and Their Anti-Gastric Ulcer Effect.

Molecules 2021 Mar 10;26(6). Epub 2021 Mar 10.

Center of Scientific Experiment, Beijing University of Chinese Medicine, Beijing 100029, China.

(GRR) is one of the commonly used traditional Chinese medicines in clinical practice, which has been applied to treat digestive system diseases for hundreds of years. GRR is preferred for anti-gastric ulcer, however, the main active compounds are still unknown. In this study, GRR was used as precursor to synthesize carbon dots (CDs) by a environment-friendly one-step pyrolysis process. GRR-CDs were characterized by using transmission electron microscopy, high-resolution TEM, fourier transform infrared, ultraviolet-visible and fluorescence spectroscopy, X-ray photoelectron spectroscopy, X-ray diffraction and high-performance liquid chromatography. In addition, cellular toxicity of GRR-CDs was studied by using CCK-8 in RAW264.7 cells, and the anti-gastric ulcer activity was evaluated and confirmed using mice model of acute alcoholic gastric ulcer. The experiment confirmed that GRR-CDs were the spherical structure with a large number of active groups on the surface and their particle size ranged from 2 to 10 nm. GRR-CDs had no toxicity to RAW264.7 cells at concentration of 19.5 to 5000 μg/mL and could reduce the oxidative damage of gastric mucosa and tissues caused by alcohol, as demonstrated by restoring expression of malondialdehyde, superoxide dismutase and nitric oxide in serum and tissue of mice. The results indicated the explicit anti-ulcer activity of GRR-CDs, which provided a new insights for the research on effective material basis of GRR.
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http://dx.doi.org/10.3390/molecules26061512DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8000522PMC
March 2021

Ocean acidification but not hypoxia alters the gonad performance in the thick shell mussel Mytilus coruscus.

Mar Pollut Bull 2021 Jun 29;167:112282. Epub 2021 Mar 29.

International Research Center for Marine Biosciences, Ministry of Science and Technology, Shanghai Ocean University, Shanghai 201306, China; Key Laboratory of Exploration and Utilization of Aquatic Genetic Resources, Ministry of Education, Shanghai Ocean University, Shanghai 201306, China. Electronic address:

Ocean acidification and hypoxia have become increasingly severe in coastal areas, and their co-occurrence poses emerging threats to coastal ecosystems. Here, we investigated the combined effects of ocean acidification and hypoxia on the reproductive capacity of the thick-shelled mussel Mytilus coruscus. Our results demonstrated low pH but not low oxygen induced decreased gonadosomatic index (GSI) in mussels. Male mussels had a lower level of sex steroids (estradiol, testosterone, and progesterone) when kept at low pH. Expression of genes related to reproduction were also impacted by low pH with a downregulation of genes involved in gonad development in males (β-catenin and Wnt-7b involved in males) and an upregulation of testosterone synthesis inhibition-related gene (Wnt-4) in females. Overall, our results suggest that ocean acidification has an impact on the gonadal development through an alternation of gene expression and level of steroids while hypoxia had no significant effect.
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http://dx.doi.org/10.1016/j.marpolbul.2021.112282DOI Listing
June 2021

Water-Soluble Carbon Dots in Cigarette Mainstream Smoke: Their Properties and the Behavioural, Neuroendocrinological, and Neurotransmitter Changes They Induce in Mice.

Int J Nanomedicine 2021 16;16:2203-2217. Epub 2021 Mar 16.

Beijing Institute of Traditional Chinese Medicine, Beijing University of Chinese Medicine, Beijing, 100029, People's Republic of China.

Background: It is well known that smoking is harmful to health; however, it can also ameliorate anxiety. To date, it is unclear whether any nanoparticles found in cigarette mainstream smoke (CS) contribute to this effect.

Aim: The aim of this study was to assess the particle composition of CS to identify novel anti-anxiety components.

Methods: Carbon dots (CDs) from CS (CS-CDs) were characterised using high-resolution transmission electron microscopy, Fourier-transform infrared, ultraviolet, fluorescence, X-ray photoelectron spectroscopy, X-ray diffraction and high-performance liquid chromatography. The anti-anxiety effects of CS-CDs in mouse models were evaluated and confirmed with the elevated plus maze and open-field tests.

Results: The quantum yield of CS-CDs was 13.74%, with a composition of C, O, and N. In addition, the surface groups contained O-H, C-H, C=O, C-N, N-H, C-O-C, and COO- bonds. Acute toxicity testing revealed that CS-CDs had low in vitro and in vivo toxicity within a certain concentration range. The results of the elevated plus maze and open-field tests showed that CS-CDs had a significant anti-anxiety effect and a certain sedative effect in mice. The mechanism of these effects may be related to the decrease in glutamate levels and promotion of norepinephrine production in the mouse brain, and the decrease in dopamine in mouse serum due to CS-CDs.

Conclusion: CS-CDs may have anti-anxiety and certain sedative effects. This study provides a new perspective for a more comprehensive understanding of the components, properties, and functions of CS. Furthermore, it offers a novel target for the development of smoking cessation treatments, such as nicotine replacement therapy.
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http://dx.doi.org/10.2147/IJN.S291670DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7982445PMC
April 2021

Ethylparaben induces subconjunctival fibrosis via the Wnt/β-catenin signaling pathway.

Exp Ther Med 2021 Apr 28;21(4):295. Epub 2021 Jan 28.

Department of Ophthalmology, Zoucheng People's Hospital, Zoucheng, Shandong 273500, P.R. China.

The aim of the present study was to explore the etiology of subconjunctival fibrosis (SCF) induced by ethylparaben, the most prevalent preservative in Chinese eye drops. Ethylparaben was administered to the left eyes of male Sprague-Dawley rats in the experimental group twice daily for 1 month, whereas the control group received PBS. Experimental group rats displayed a mild promotion in density of fibroblasts and a tighter deposition of collagen in the bulbar subepithelial connective tissue compared with the control group. Furthermore, the present findings revealed that extracellular matrix expression was promoted in murine bulbar conjunctival tissues in the experimental group. In primary conjunctival fibroblasts, expression of ECM triggered by ethylparaben was suppressed by XAV-939. Furthermore, stimulation of the Wnt/β-catenin axis triggered by ethylparaben was impaired by XAV-939. In conclusion, SCF triggered by ethylparaben results from extra ECM generation of conjunctival fibroblasts via the Wnt/β-catenin axis.
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http://dx.doi.org/10.3892/etm.2021.9726DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7885073PMC
April 2021

A 3D Silverton-Type Polyoxomolybdate Based on {PrMoO}: Synthesis, Structure, Photoluminescence and Magnetic Properties.

Front Chem 2021 19;9:615595. Epub 2021 Feb 19.

Henan Key Laboratory of Polyoxometalate Chemistry, Institute of Molecular and Crystal Engineering, College of Chemistry and Chemical Engineering, Henan University, Kaifeng, China.

A three-dimensional (3D) Silverton-type polyoxomolybdate (POMo) with the formula of NH{Mn[PrMoO]}·18HO (1) was successfully isolated and well characterized by single crystal X-ray diffraction, X-ray powder diffraction pattern, infrared spectrum, thermogravimetric and elemental analyses. The inorganic building block {PrMoO} has formed 3D frameworks the {MnO} linker. The excitation of compound 1 in solid state at 375 nm displays red emission. Moreover, variable temperature magnetic susceptibility measurements indicate that the magnetic behavior in compound 1 is dominated by antiferromagnetic interactions.
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http://dx.doi.org/10.3389/fchem.2021.615595DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7935544PMC
February 2021

Ecological risk assessment of trace metals in sediments under reducing conditions based on isotopically exchangeable pool.

J Environ Sci Health A Tox Hazard Subst Environ Eng 2021 24;56(2):171-180. Epub 2020 Dec 24.

School of Environment and Architecture, University of Shanghai for Science and Technology, Shanghai, China.

Determination of potential mobility of toxic trace metals in sediments under changing redox condition is important in ecological risk assessment. Current methods are limited in risk prediction in such dynamic environment. In this study, we have discussed the general disagreement from widely used methods (sediment quality guideline (SQGs), potential ecological risk index (PERI), risk assessment code (RAC) using BCR fraction information). In addition, the stable isotopic dilution method (IDM) was also modified to quantify metal lability in a microcosm experiment mimicking river bank sediment turning into anaerobic. The isotopically exchangeable Cd, Cu, Pb, and Zn quantified by IDM ( ) was used in the RAC to reveal the trend of risk during this process. Strong risks from Cd are suggested by the PERI and RAC as a result of high toxicity and mobility of the element, while SQGs suggests medium risk for Cu, Pb, and Zn in certain samples. The disagreement between the results of RAC assessed by metal lability ( ) and by BCR metal fractionation reflects the effect of sediment properties and source of metal contamination. The RAC based on the non-residual fractions is likely to overestimate the potential risk for most metals even there is a significant change in sediment Eh. The RAC assessed by reveals that the variability in risk in response to the reducing Eh is not consistent. Large fluctuation in for Cd (28.5%, 49.5%), Pb (27.6%, 18.2%), and Cu (14.4%, 24.7%) can shift the risks to a higher level in certain range of Eh in two sediments. In sediment with lower contents of metal binding phases (e.g. mineral oxides, organic matters), the release of metals can be more significant, thus higher ecological risk in changing redox condition.
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http://dx.doi.org/10.1080/10934529.2020.1857154DOI Listing
February 2021

Carbon dots from Artemisiae Argyi Folium Carbonisata: strengthening the anti-frostbite ability.

Artif Cells Nanomed Biotechnol 2021 Dec;49(1):11-19

Beijing Key Laboratory, School of Traditional Chinese Medicine, Beijing University of Chinese Medicine, Beijing, China.

In this study, novel carbon dots (CDs) were discovered and separated from (AAFC) aqueous extract. AAFC-CDs were characterised by a series of methods, mainly including electron microscopy, optical technology and X-ray photoelectron spectroscopy (XPS). Results displayed that AAFC-CDs with a quantum yield (QY) around 0.19% had a size distribution between 6.0 and 10.0 nm and possessed a nearly spherical shape, with a lattice spacing of 0.369 nm. In mice, AAFC-CDs reduced the tissue damage, ear frostbite, and body stiffness caused by cold, and provided energy by increasing the use of blood glucose. The mechanism may be by decreasing concentration of IL-1βk, TNF-α and reducing the rise in blood glucose levels caused by frostbite. This study is the first to indicate that CDs may be the active constituent of AAFC against frostbite, suggesting their potential for clinical applications.
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http://dx.doi.org/10.1080/21691401.2020.1862134DOI Listing
December 2021

Thermodynamic Analysis of Methylcyclohexane Dehydrogenation and Solar Energy Storage via Solar-Driven Hydrogen Permeation Membrane Reactor.

Membranes (Basel) 2020 Nov 27;10(12). Epub 2020 Nov 27.

MOE Key Laboratory of Hydrodynamic Transients, School of Power and Mechanical Engineering, Wuhan University, Wuhan 430072, China.

A novel methylcyclohexane (MCH) dehydrogenation system driven by solar energy with a hydrogen permeation membrane (HPM) reactor is proposed in this study. It is a promising method, via this novel system, to generate pure hydrogen and store intermittent solar energy. In this research, the thermodynamic analysis of MCH dehydrogenation via the HPM reactor was conducted based on numerical simulation. The conversion rates and thermodynamic efficiencies under different temperatures (150-350 °C), permeate pressures from 0.001 to 0.5 bar, and solar irradiation in the four seasons were studied and analyzed. Under a hydrogen partial pressure difference, HPM can separate hydrogen and shift the reaction equilibrium forward for a higher conversion rate of MCH, which can reach nearly 99.7% in this system. The first-law of thermodynamic efficiency, the solar-to-fuel efficiency, and the exergy efficiency are up to 95.58%, 38.65%, and 94.22%, respectively. This study exhibits the feasibility and potential of MCH dehydrogenation via the HPM reactor driven by solar energy and provides a novel approach for solar energy storage.
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http://dx.doi.org/10.3390/membranes10120374DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7760799PMC
November 2020

Antioxidant responses of the mussel Mytilus coruscus co-exposed to ocean acidification, hypoxia and warming.

Mar Pollut Bull 2021 Jan 27;162:111869. Epub 2020 Nov 27.

International Research Center for Marine Biosciences, Ministry of Science and Technology, Shanghai Ocean University, Shanghai 201306, China; Key Laboratory of Exploration and Utilization of Aquatic Genetic Resources, Ministry of Education, Shanghai Ocean University, Shanghai 201306, China. Electronic address:

In the present study, the combined effects of pH, dissolved oxygen (DO) and temperature levels on the antioxidant responses of the mussel Mytilus coruscus were evaluated. Mussels were exposed to two pH (8.1, 7.7-acidification), two DO (6 mg L, 2 mg L-hypoxia) and two temperature levels (20 °C, 30 °C-warming) for 30 days. SOD, CAT, MDA, GPx, GSH, GST, TAOC, AKP, ACP, GPT, AST levels were measured in the gills of mussels. All tested biochemical parameters were altered by these three environmental stressors. Values for all the test parameters except GSH first increased and then decreased at various experimental treatments during days 15 and 30 as a result of acidification, hypoxia and warming. GSH content always increased with decreased pH, decreased DO and increased temperature. PCA showed a positive correlation among all the measured biochemical indexes. IBR results showed that M. coruscus were adversely affected by reduced pH, low DO and elevated temperature.
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http://dx.doi.org/10.1016/j.marpolbul.2020.111869DOI Listing
January 2021

Carbon Dots from Carbonisata: Hepatoprotective Effect.

Int J Nanomedicine 2020 17;15:9049-9059. Epub 2020 Nov 17.

School of Traditional Chinese Medicine, Beijing University of Chinese Medicine, Beijing 100029, People's Republic of China.

Introduction: The charcoal processed product of (PRA), PRA Carbonisata (PRAC), has long been used for its hepatoprotective effects. However, the material basis and mechanism of action of PRAC remain unclear.

Aim: To explore the hepatoprotective effects of Carbonisata-derived carbon dots (PRAC-CDs).

Methods: PRAC-CDs were characterized using transmission electron microscopy, high-resolution transmission electron microscopy, ultraviolet, fluorescence, Fourier transform infrared and X-ray photoelectron spectroscopy, X-ray diffraction, and high-performance liquid chromatography. The hepatoprotective effect of PRAC-CDs was evaluated and confirmed using the classic carbon tetrachloride acute liver injury model.

Results: PRAC-CDs averaged 1.0-2.4 nm in size and exhibited a quantum yield of 5.34% at a maximum excitation wavelength of 320 nm and emission at 411 nm. PRAC-CDs can reduce the ALT and AST levels of mice with carbon tetrachloride-induced acute liver injury and have a mitigating effect on the rise in TBA and TBIL. More interestingly, PRAC-CDs can significantly reduce MDA and increase SOD levels, demonstrating that PRAC-CDs can improve the body's ability to scavenge oxygen free radicals and inhibit free radical-induced liver cell lipid peroxidation, thereby preventing liver cell damage.

Conclusion: These results demonstrate the remarkable hepatoprotective effects of PRAC-CDs against carbon tetrachloride-induced acute liver injury, which provide new insights into potential biomedical and healthcare applications of CDs.
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http://dx.doi.org/10.2147/IJN.S281976DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7680119PMC
December 2020

Non-precious-metal catalysts for alkaline water electrolysis: operando characterizations, theoretical calculations, and recent advances.

Chem Soc Rev 2020 Dec 3;49(24):9154-9196. Epub 2020 Nov 3.

Department of Chemistry, Seoul National University, Seoul, South Korea.

Recent years have witnessed an upsurge in the development of non-precious catalysts (NPCs) for alkaline water electrolysis (AWE), especially with the strides made in experimental and computational techniques. In this contribution, the most recent advances in NPCs for AWE were systematically reviewed, emphasizing the application of in situ/operando experimental methods and density functional theory (DFT) calculations in their understanding and development. First, we briefly introduced the fundamentals of the anode and cathode reaction for AWE, i.e., the oxygen evolution reaction (OER) and the hydrogen evolution reaction (HER), respectively. Next, the most popular in situ/operando approaches for characterizing AWE catalysts, including hard and soft XAS, ambient-pressure XPS, liquid and identical location TEM, electrochemical mass spectrometry, and Raman spectroscopy were thoroughly summarized. Subsequently, we carefully discussed the principles, computational methods, applications, and combinations of DFT with machine learning for modeling NPCs and predicting the alkaline OER and HER. With the improved understanding of the structure-property-performance relationship of NPCs for AWE, we proceeded to overview their current development, summarising state-of-the-art design strategies to boost their activity. In addition, advances in various extensively investigated NPCs for AWE were evaluated. By conveying these methods, progress, insights, and perspectives, this review will contribute to a better understanding and rational development of non-precious AWE electrocatalysts for hydrogen production.
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http://dx.doi.org/10.1039/d0cs00575dDOI Listing
December 2020

Carcinoembryonic Antigen: A Potential Biomarker to Evaluate the Severity and Prognosis of COVID-19.

Front Med (Lausanne) 2020 6;7:579543. Epub 2020 Oct 6.

Department of General Surgery, The First Affiliated Hospital of Nanjing Medical University, Nanjing, China.

Corona Virus Disease 2019 (COVID-19) has become a serious pandemic disease worldwide. Identification of biomarkers to predict severity and prognosis is urgently needed for early medical intervention due to high mortality of critical cases with COVID-19. This retrospective study aimed to indicate the values of carcinoembryonic antigen (CEA) in evaluating the severity and prognosis of COVID-19. We included 46 death cases from intensive care unit and 68 discharged cases from ordinary units with confirmed COVID-19 of Wuhan Jin Yin-tan Hospital from January 1 to March 22, 2020. Laboratory and radiologic data were analyzed retrospectively. All patients were followed up until April 10, 2020. COVID-19 patients in the death group had significantly higher CEA levels (ng/ml) than discharged group (14.80 ± 14.20 vs. 3.80 ± 2.43, < 0.001). The risk of COVID-19 death increased 1.317 times for each additional 1 ng/ml CEA level (OR = 1.317, 95% CI: 1.099-1.579). The standardized and weighted receiver operating characteristic curve (ROC) analysis adjusted to age, sex, and ferritin levels suggested that the area under the curve (AUC) of the serum CEA levels was 0.808 in discrimination between death cases and discharged cases with COVID-19 ( < 0.001). We found mortality of COVID-19 is associated with elevated CEA levels increased (HR = 1.023, 95% CI: 1.005-1.042), as well as age (HR = 1.050, 95% CI: 1.016-1.086) and ferritin levels (HR = 1.001, 95% CI: 1.001-1.002) by survival analysis of Cox regression model. Among discharged patients, CEA levels were significant lower in moderate cases compared to the severe and critical cases ( = 0.005; OR = 0.488, 95% CI: 0.294-0.808) from binary logistic regression analysis. The AUC of CEA levels was 0.79 in distinguishing moderate cases from discharged COVID-19 patients by standardized and weighted ROC analysis ( < 0.001). A positive correlation between CEA levels and CT scores existed in discharged patients (Correlation Coefficient: 0.687; < 0.001). Elevated CEA levels increased the risk of death from COVID-19 and CEA levels were related to CT scores of the discharged patients positively.
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http://dx.doi.org/10.3389/fmed.2020.579543DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7573292PMC
October 2020

Seroepidemiology of TORCH antibodies in the reproductive-aged women in China.

Eur J Obstet Gynecol Reprod Biol 2020 Nov 7;254:114-118. Epub 2020 Sep 7.

Virology Laboratory, Shengjing Hospital of China Medical University, Shenyang, 110004, PR China. Electronic address:

TORCH, the acronym of Toxoplasma gondii (TOX), others, rubella virus (RUV), cytomegalovirus (CMV) and herpes simplex virus (HSV), is a major contributor to congenital infection. National population-based study on the seroepidemiology of TORCH in women is yet lacking, and it is still obscure whether TORCH infection in the women was associated with adverse pregnancy outcomes. A total of 48,406 asymptomatic women from eight hospitals in China which covered the most areas of mainland China were enrolled in this study, and 26,400 were simultaneously subjected to 7 detection tests for TORCH specific antibodies. Chemiluminescent immunoassay was performed to detect TORCH Immunoglobulin M (IgM) and/or Immunoglobulin G (IgG) antibodies, and IgG avidities of TOX and CMV IgM and IgG positive serum samples. The overall IgG prevalence of TOX, RUV, CMV and HSV-(1 + 2) in the reproductive-aged women was 1.71 %, 81.97 %, 95.09 % and 90.15 % respectively. The corresponding IgM prevalence of TOX, RUV and CMV was 0.30 %, 0.89 % and 0.52 %. Moreover, the rates of primary TOX and CMV infections were at least 0.08 % (21/26,400) and 0.03 % (7/26,400) in the studied population. The distributions of TORCH positive women in various age, season and region groups were different (P < 0.05). The CMV IgM-positive rate was higher in the pregnant women than those in non-pregnant women (P < 0.05). The higher past infection rates of RUV, CMV and HSV in women with bad obstetric history (BOH) imply that TORCH infections are associated with BOH. These data suggest that TORCH infections in the prenatal women, especially with BOH, are worthwhile to be screened by detections of specific IgG and IgM antibodies, and even IgG avidities.
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http://dx.doi.org/10.1016/j.ejogrb.2020.09.010DOI Listing
November 2020

[Prenatal genetic diagnosis of a partial 21 trisomy fetus with nasal bone dysplasia].

Zhonghua Yi Xue Yi Chuan Xue Za Zhi 2020 Oct;37(10):1172-1175

Women and Children's Hospital Affiliated to Xiamen University, Central Laboratory of Xiamen Maternal and Child Health Care Hospital, Xiamen, Fujian 361003, China.

Objective: To explore the nature of chromosomal abnormality in a fetus with nasal bone dysplasia and clarify its clinical effect.

Methods: Fetal chromosome karyotype was analyzed by G-banding. Single nucleotide polymorphism array (SNP-array) was used to detect the chromosomal copy number variations, and fluorescence in situ hybridization (FISH) was used to verify the result.

Results: Fetal karyotype analysis showed an unknown chromosomal fragment in 21q21 region. SNP-array discovered a 7.5 Mb duplication in the 21q22.12q22.3 region. FISH confirmed that the unknown fragment was derived from a 21q22.12q22.3 duplication.

Conclusion: Combined use of karyotype analysis, SNP-array and FISH has clarified the nature of chromosomal abnormality in a fetus with nasal bone dysplasia, which has enabled more accurate prenatal diagnosis and genetic counseling.
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http://dx.doi.org/10.3760/cma.j.cn511374-20190605-00282DOI Listing
October 2020

Dentin Sialophosphoprotein Deletion Leads to Femoral Head Cartilage Attenuation and Subchondral Bone Ill-mineralization.

J Histochem Cytochem 2020 10 14;68(10):703-718. Epub 2020 Sep 14.

Department of Oral Pathology, School and Hospital of Stomatology, China Medical University, Shenyang, China.

Dentin sialophosphoprotein (DSPP), which expresses and synthesizes in odontoblasts of dental pulp, is a critical protein for normal teeth mineralization. Originally, DSPP was identified as a dentin-specific protein. In 2010, DSPP was also found in femoral head cartilage, and it is still unclear what roles DSPP play in femoral head cartilage formation, growth, and maintenance. To reveal biological functions of DSPP in the femoral head cartilage, we examined null mice compared with wild-type (WT) mice to observe DSPP expression as well as localization in WT mice and to uncover differences of femoral head cartilage, bone morphology, and structure between these two kinds of mice. Expression data demonstrated that DSPP had heterogeneous fragments, expressed in each layer of femoral head cartilage and subchondral bone of WT mice. null mice exhibited a significant reduction in the thickness of femoral head cartilage, with decreases in the amount of proliferating cartilage cells and increases in apoptotic cells. In addition, the subchondral bone mineralization decreased, and the expressions of vessel markers (vascular endothelial growth factor [VEGF] and CD31), osteoblast markers (Osterix and dentin matrix protein 1 [DMP1]), osteocyte marker (sclerostin [SOST]), and osteoclast marker (tartrate-resistant acid phosphatase [TRAP]) were remarkably altered. These indicate that DSPP deletion can affect the proliferation of cartilage cells in the femoral head cartilage and endochondral ossification in subchondral bone. Our data clearly demonstrate that DSPP plays essential roles in the femoral head cartilage growth and maintenance and subchondral biomineralization.
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http://dx.doi.org/10.1369/0022155420960403DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7534101PMC
October 2020
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