Publications by authors named "Hui He"

577 Publications

Reconfiguration of Functional Dynamics in Cortico-Thalamo-Cerebellar Circuit in Schizophrenia Following High-Frequency Repeated Transcranial Magnetic Stimulation.

Front Hum Neurosci 2022 25;16:928315. Epub 2022 Jul 25.

The Clinical Hospital of Chengdu Brain Science Institute, MOE Key Lab for Neuroinformation, School of Life Sciences and Technology, University of Electronic Science and Technology of China, Chengdu, China.

Schizophrenia is a serious mental illness characterized by a disconnection between brain regions. Transcranial magnetic stimulation is a non-invasive brain intervention technique that can be used as a new and safe treatment option for patients with schizophrenia with drug-refractory symptoms, such as negative symptoms and cognitive impairment. However, the therapeutic effects of transcranial magnetic stimulation remain unclear and would be investigated using non-invasive tools, such as functional connectivity (FC). A longitudinal design was adopted to investigate the alteration in FC dynamics using a dynamic functional connectivity (dFC) approach in patients with schizophrenia following high-frequency repeated transcranial magnetic stimulation (rTMS) with the target at the left dorsolateral prefrontal cortex (DLPFC). Two groups of schizophrenia inpatients were recruited. One group received a 4-week high-frequency rTMS together with antipsychotic drugs (TSZ, = 27), while the other group only received antipsychotic drugs (DSZ, = 26). Resting-state functional magnetic resonance imaging (fMRI) and psychiatric symptoms were obtained from the patients with schizophrenia twice at baseline () and after 4-week treatment (). The dynamics was evaluated using voxel- and region-wise FC temporal variability resulting from fMRI data. The pattern classification technique was used to verify the clinical application value of FC temporal variability. For the voxel-wise FC temporary variability, the repeated measures ANCOVA analysis showed significant treatment × time interaction effects on the FC temporary variability between the left DLPFC and several regions, including the thalamus, cerebellum, precuneus, and precentral gyrus, which are mainly located within the cortico-thalamo-cerebellar circuit (CTCC). For the ROI-wise FC temporary variability, our results found a significant interaction effect on the FC among CTCC. rTMS intervention led to a reduced FC temporary variability. In addition, higher alteration in FC temporal variability between left DLPFC and right posterior parietal thalamus predicted a higher remission ratio of negative symptom scores, indicating that the decrease of FC temporal variability between the brain regions was associated with the remission of schizophrenia severity. The support vector regression (SVR) results suggested that the baseline pattern of FC temporary variability between the regions in CTCC could predict the efficacy of high-frequency rTMS intervention on negative symptoms in schizophrenia. These findings confirm the potential relationship between the reduction in whole-brain functional dynamics induced by high-frequency rTMS and the improvement in psychiatric scores, suggesting that high-frequency rTMS affects psychiatric symptoms by coordinating the heterogeneity of activity between the brain regions. Future studies would examine the clinical utility of using functional dynamics patterns between specific brain regions as a biomarker to predict the treatment response of high-frequency rTMS.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.3389/fnhum.2022.928315DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9359206PMC
July 2022

Virioplankton assemblages from challenger deep, the deepest place in the oceans.

iScience 2022 Aug 27;25(8):104680. Epub 2022 Jun 27.

College of Marine Life Sciences, Institute of Evolution and Marine Biodiversity, Frontiers Science Center for Deep Ocean Multispheres and Earth System, Key Lab of Polar Oceanography and Global Ocean Change, Ocean University of China, Qingdao 266003, China.

Hadal ocean biosphere, that is, the deepest part of the world's oceans, harbors a unique microbial community, suggesting a potential uncovered co-occurring virioplankton assemblage. Herein, we reveal the unique virioplankton assemblages of the Challenger Deep, comprising 95,813 non-redundant viral contigs from the surface to the hadal zone. Almost all of the dominant viral contigs in the hadal zone were unclassified, potentially related to Alteromonadales and Oceanospirillales. 2,586 viral auxiliary metabolic genes from 132 different KEGG orthologous groups were mainly related to the carbon, nitrogen, sulfur, and arsenic metabolism. Lysogenic viral production and integrase genes were augmented in the hadal zone, suggesting the prevalence of viral lysogenic life strategy. Abundant rve genes in the hadal zone, which function as transposase in the caudoviruses, further suggest the prevalence of viral-mediated horizontal gene transfer. This study provides fundamental insights into the virioplankton assemblages of the hadal zone, reinforcing the necessity of incorporating virioplankton into the hadal biogeochemical cycles.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.isci.2022.104680DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9356048PMC
August 2022

Cyst stem cell lineage eIF5 non-autonomously prevents testicular germ cell tumor formation via eIF1A/eIF2γ-mediated pre-initiation complex.

Stem Cell Res Ther 2022 07 26;13(1):351. Epub 2022 Jul 26.

Institute of Reproductive Medicine, Medical School, Nantong University, Nantong, 226001, China.

Background: Stem cell niche maintains stem cell population identity and is essential for the homeostasis of self-renewal and differentiation in Drosophila testes. However, the mechanisms of CySC lineage signals-mediated soma-germline communications in response to external stimuli are unclear.

Methods: Pre-initiation complex functions were evaluated by UAS-Gal4-mediated cell effects. RNA sequencing was conducted in NC and eIF5 siRNA-treated cells. Genetic interaction analysis was used to indicate the relationships between eIF5 and eIF1A/eIF2γ in Drosophila testes.

Results: Here, we demonstrated that in CySCs, translation initiation factor eIF5 mediates cyst cell differentiation and the non-autonomously affected germ cell differentiation process. CySCs lacking eIF5 displayed unbalanced cell proliferation and apoptosis, forming testicular germ cell tumors (TGCTs) during spermatogenesis. eIF5 transcriptional regulation network analysis identified multiple metabolic processes and several key factors that might be involved in germ cell differentiation and TGCT formation. Importantly, knockdown of eIF1A and eIF2γ, key components of pre-initiation complex, mimicked the phenotype of knocking down eIF5 in the stem cell niche of Drosophila testes. Genetic interaction analysis indicated that eIF5 was sufficient to rescue the phenotype of tumorlike structures induced by down-regulating eIF1A or eIF2γ in CySCs.

Conclusions: These findings demonstrated that CySC lineage eIF5, together with eIF1A or eIF2γ, mediates soma-germline communications for the stem cell niche homeostasis in Drosophila testes, providing new insights for the prevention of TGCTs.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1186/s13287-022-03025-5DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9327282PMC
July 2022

Effects of black carbon aerosol on air quality and vertical meteorological factors in early summer in Beijing.

Sci Total Environ 2022 Jul 21;847:157529. Epub 2022 Jul 21.

School of Earth and Space Sciences, University of Science and Technology of China, Hefei, China.

Black carbon (BC) aerosols have effects on the atmospheric thermal vertical structure due to its radiation absorption characteristics, hereby influencing the boundary layer characteristics and pollutant diffusion. This study focuses on the BC effects under different atmospheric conditions on air quality and vertical meteorological conditions. Four days flight observation combined with surface wind profiler radar data were used to investigate the vertical profiles of BC and wind speed over Beijing urban area in early summer. The vertical profiles of BC concentration and wind speed in the boundary layer had a negative correlation, both having abrupt changes near the boundary layer height under stagnant weather conditions. The chemical transport model showed the increase of BC under stagnant conditions could cause aggravation of the stability of the boundary layer, thereby increasing the accumulation of pollutants. In particular, BC leads to the changes in the temperature profile, which will modify relative humidity and indirectly lead to the changes in the vertical profile of aerosol optical properties. However, if the early accumulation of BC was absent under more turbulent conditions, the effects of BC on air quality and meteorological conditions were limited.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.scitotenv.2022.157529DOI Listing
July 2022

Characterization and Genomic Analysis of ssDNA Vibriophage vB_VpaM_PG19 within , Representing a Novel Viral Genus.

Microbiol Spectr 2022 Jul 6:e0058522. Epub 2022 Jul 6.

College of Marine Life Sciences, Frontiers Science Center for Deep Ocean Multispheres and Earth System, Institute of Evolution and Marine Biodiversity, Ocean University of Chinagrid.4422.0, Qingdao, China.

Vibrio parahaemolyticus, a widespread marine bacterium, is responsible for a variety of diseases in marine organisms. Consumption of raw or undercooked seafood contaminated with V. parahaemolyticus is also known to cause acute gastroenteritis in humans. While numerous dsDNA vibriophages have been isolated so far, there have been few studies of vibriophages belonging to the ssDNA family. In this study, a novel ssDNA phage, vB_VpaM_PG19 infecting V. parahaemolyticus, with a 5,572 bp ssDNA genome with a G+C content of 41.31% and encoded eight open reading frames, was isolated. Genome-wide phylogenetic analysis of the total phage isolates in the GenBank database revealed that vB_VpaM_PG19 was only related to the recently deposited vibriophage vB_VpP_WS1. The genome-wide average nucleotide homology of the two phages was 89.67%. The phylogenetic tree and network analysis showed that vB_VpaM_PG19 was different from other members of the family and might represent a novel viral genus, together with vibriophage vB_VpP_WS1, named . One-step growth curves showed that vB_VpaM_PG19 has a short incubation period, suggesting its potential as an antimicrobial agent for pathogenic V. parahaemolyticus. Vibriophage vB_VpaM_PG19 was distant from other isolated microviruses in the phylogenetic tree and network analysis and represents a novel microviral genus, named . Our report describes the genomic and phylogenetic features of vB_VpaM_PG19 and provides a potential antimicrobial candidate for pathogenic V. parahaemolyticus.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1128/spectrum.00585-22DOI Listing
July 2022

EEG Evidence of Altered Functional Connectivity and Microstate in Children Orphaned by HIV/AIDS.

Front Psychiatry 2022 29;13:898716. Epub 2022 Jun 29.

Department of Health Promotion, Education, and Behavior, University of South Carolina, Columbia, SC, United States.

Children orphaned by HIV/AIDS ("AIDS orphans") suffer numerous early-life adverse events which have a long-lasting effect on brain function. Although previous studies found altered electroencephalography (EEG) oscillation during resting state in children orphaned by HIV/AIDS, data are limited regarding the alterations in connectivity and microstate. The current study aimed to investigate the functional connectivity (FC) and microstate in children orphaned by HIV/AIDS with resting-state EEG data. Data were recorded from 63 children orphaned by HIV/AIDS and 65 non-orphan controls during a close-eyes resting state. The differences in phase-locking value (PLV) of global average FC and temporal dynamics of microstate were compared between groups. For functional connectivity, children orphaned by HIV/AIDS showed decreased connectivity in alpha, beta, theta, and delta band compared with non-orphan controls. For microstate, EEG results demonstrated that children orphaned by HIV/AIDS show increased duration and coverage of microstate C, decreased occurrence and coverage of microstate B, and decreased occurrence of microstate D than non-orphan controls. These findings suggest that the microstate and functional connectivity has altered in children orphaned by HIV/AIDS compared with non-orphan controls and provide additional evidence that early life stress (ELS) would alter the structure and function of the brain and increase the risk of psychiatric disorders.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.3389/fpsyt.2022.898716DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9277056PMC
June 2022

Using honeypots to model botnet attacks on the internet of medical things.

Comput Electr Eng 2022 Sep 8;102:108212. Epub 2022 Jul 8.

Department of Computer Science and Technology, Harbin Institute of Technology, Shenzhen, China.

Corona Virus Disease 2019 (COVID-19) has led to an increase in attacks targeting widespread smart devices. A vulnerable device can join multiple botnets simultaneously or sequentially. When different attack patterns are mixed with attack records, the security analyst produces an inaccurate report. There are numerous studies on botnet detection, but there is no publicly available solution to classify attack patterns based on the control periods. To fill this gap, we propose a novel data-driven method based on an intuitive hypothesis: bots tend to show time-related attack patterns within the same botnet control period. We deploy 462 honeypots in 22 countries to capture real-world attack activities and propose an algorithm to identify control periods. Experiments have demonstrated our method's efficacy. Besides, we present eight interesting findings that will help the security community better understand and fight botnet attacks now and in the future.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.compeleceng.2022.108212DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9264116PMC
September 2022

SAT2 regulates Sertoli cell-germline interactions via STIM1-mediated ROS/WNT/β-catenin signaling pathway.

Cell Biol Int 2022 Jul 12. Epub 2022 Jul 12.

State Key Laboratory of Reproductive Medicine, Center for Reproduction and Genetics, Suzhou Municipal Hospital, The Affiliated Suzhou Hospital of Nanjing Medical University, Gusu School, Nanjing Medical University, Suzhou, China.

As the main component of seminiferous tubules, Sertoli cells are in close contact with germ cells and generate niche signals, which exhibit pivotal functions in spermatogenesis and male fertility. However, the regulatory mechanisms of Sertoli cell-germline interactions (SGIs) in the testes of neonatal mice (NM) remain largely unclear. Previously, we identified spermidine/spermine N1-acetyl transferase 2 (SAT2) and stromal interaction molecule 1 (STIM1) to be potential regulators of testicular cord formation via comparative proteomics analysis. Here, we demonstrated a novel role of SAT2 for SGIs during testicular development in NM. Testicular explants lacking SAT2 affected the mislocation, but not the quantity, of Sertoli cells, which led to maintenance defects in spermatogonial stem cells (SSCs). Interestingly, SAT2 was essential for the migration of TM4 cells, a Sertoli cell line. Mechanistically, SAT2 was able to bind STIM1, repress its expression, and regulate homeostasis of a reactive oxygen species/wingless type (WNT)/β-catenin pathway in NM testes. Collectively, our study identified that SAT2 was able to regulate SGIs via a STIM1-mediated WNT signaling pathway.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1002/cbin.11857DOI Listing
July 2022

Effect of Exercise Training on Body Composition and Inflammatory Cytokine Levels in Overweight and Obese Individuals: A Systematic Review and Network Meta-Analysis.

Front Immunol 2022 23;13:921085. Epub 2022 Jun 23.

China Institute of Sport and Health Science, Beijing Sport University, Beijing, China.

Objective: This study aimed to compare and rank the effectiveness of aerobic exercise (AE), resistance training (RT), combined aerobic and resistance training (CT), and high-intensity interval training (HIIT) on body composition and inflammatory cytokine levels in overweight and obese individuals by using network meta-analysis (NMA).

Methods: We searched the PubMed, Cochrane, Embase, Web of Science, and EBSCO databases to identify randomized controlled trials investigating the effects of exercise training on inflammatory cytokines in overweight and obese patients. The retrieval period was from inception to November 2021. Two reviewers independently screened the retrieved articles, extracted the pertinent data, and assessed the risk of bias of the included studies; then, they used Stata 16.0 and Review Manager 5.3 to perform an NMA.

Results: A total of 38 studies involving 1317 patients were included in this study. The results of the NMA indicated that AE had the greatest effect on weight loss (SUCRA=78.3; SMD=-0.51, 95% CI: -0.70, -0.33); CT had the greatest effect on reducing body mass index (SUCRA=70.7; SMD=-0.46, 95% CI: -0.81, -0.10), waist circumference (SUCRA=93.4; SMD=-1.86, 95% CI: -2.80, -0.93), percentage body fat (SUCRA=79.6; SMD=-1.38, 95% CI: -2.29, -0.48), interleukin-6 level (SUCRA=86.4; SMD=-1.98, 95% CI: -3.87, -0.09), and tumor necrosis factor-α level (SUCRA=79.4; SMD=-2.08, 95% CI: -3.75, -0.42); AE (SMD=0.51, 95% CI: -1.68, 2.69), RT (SMD=0.15, 95% CI: -3.01, 3.32), CT (SMD=1.78, 95% CI: -1.35, 4.92), and HIIT (SMD=2.29, 95% CI: -1.27, 5.86) did not significantly increase the adiponectin level.

Conclusion: The current results suggest that CT is the best exercise modality for improving body composition and inflammatory status in overweight and obese individuals. More rigorous randomized control trials are needed for further validation.

Systematic Review Registration: https://www.crd.york.ac.uk/prospero/, identifier CRD42022303165.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.3389/fimmu.2022.921085DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9260601PMC
July 2022

Changes of Sensory Quality, Flavor-Related Metabolites and Gene Expression in Peach Fruit Treated by Controlled Atmosphere (CA) under Cold Storage.

Int J Mol Sci 2022 Jun 27;23(13). Epub 2022 Jun 27.

Laboratory of Fruit Quality Biology Zhejiang Provincial Key/Laboratory of Horticultural Plant Integrative Biology, Zhejiang University, Hangzhou 310058, China.

Controlled atmosphere (CA) has been used to alleviate chilling injury (CI) of horticultural crops caused by cold storage. However, the effects of CA treatment on peach fruit sensory quality and flavor-related chemicals suffering from CI remain largely unknown. Here, we stored peach fruit under CA with 5% O and 10% CO at 0 °C up to 28 d followed by a subsequent 3 d shelf-life at 20 °C (28S3). CA significantly reduced flesh browning and improved sensory quality at 28S3. Though total volatiles declined during extended cold storage, CA accumulated higher content of volatile esters and lactones than control at 28S3. A total of 14 volatiles were positively correlated with consumer acceptability, mainly including three C6 compounds, three esters and four lactones derived from the fatty acid lipoxygenase (LOX) pathway. Correspondingly, the expression levels of genes including , hyperoxide lyase and alcohol acyltransferase were positively correlated with the change of esters and lactones. CA elevated the sucrose content and the degree of fatty acids unsaturation under cold storage, which gave us clues to clarify the mechanism of resistance to cold stress. The results suggested that CA treatment improved sensory quality by alleviating CI of peach fruits under cold storage.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.3390/ijms23137141DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9266655PMC
June 2022

Construction of Metabolic Molecular Classification and Immune Characteristics for the Prognosis Prediction of Ovarian Cancer.

J Immunol Res 2022 25;2022:2359349. Epub 2022 Jun 25.

Department of Reproductive Medicine, The First Affiliated Hospital of Harbin Medical University, Harbin City, Heilongjiang Province, China 150001.

Background: Ovarian cancer (OC) is a malignant tumor that seriously threatens women's health. Molecular classification based on metabolic genes can reflect the deeper characteristics of ovarian cancer and provide support for prognostic evaluation and the guidance of individualized treatment.

Method: The metabolic subtypes were determined by consensus clustering and CDF. We used the ssGSEA method to calculate the IFN score of each patient. The CIBERSORT method was used to evaluate the score distribution and differential expression of 22 immune cells, and LDA was applied to establish a subtype classification feature index. The Kaplan-Meier and ROC curves were generated to validate the prognostic performance of metabolic subtypes in different cohorts. WGCNA was used to screen the coexpression modules associated with metabolic genes.

Results: We obtained three metabolic subtypes (MC1, MC2, and MC3). MC2 had the best prognosis, and MC1 and MC3 had poor prognoses. Consistently, MC2 subtype had higher T cell lytic activity and lower angiogenesis, IFN, T cell dysfunction, and rejection scores. TIDE analysis showed that MC2 patients were more likely to benefit from immunotherapy; MC1 patients were more sensitive to immune checkpoint inhibitors and traditional chemotherapy drugs. The multiclass AUCs based on the RNASeq and GSE cohorts were 0.93 and 0.84, respectively. Finally, we screened 11 potential gene markers related to the metabolic characteristic index that could be used to indicate the prognosis of OC.

Conclusion: Molecular subtypes related to metabolism are crucial to comprehensively understand the molecular pathological characteristics related to metabolism for OC development, explore reliable markers for prognosis, improve the OC staging system, and guide personalized treatment.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1155/2022/2359349DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9253871PMC
July 2022

Efficient self-attention mechanism and structural distilling model for Alzheimer's disease diagnosis.

Comput Biol Med 2022 Aug 17;147:105737. Epub 2022 Jun 17.

The Key Laboratory for NeuroInformation of Ministry of Education, High-Field Magnetic Resonance Brain Imaging Key Laboratory of Sichuan Province, Center for Information in Medicine, University of Electronic Science and Technology of China, Chengdu, 610054, China.

Structural magnetic resonance imaging (sMRI) is commonly used for the identification of Alzheimer's disease because of its keen insight into atrophy-induced changes in brain structure. Current mainstream convolutional neural network-based deep learning methods ignore the long-term dependencies between voxels; thus, it is challenging to learn the global features of sMRI data. In this study, an advanced deep learning architecture called Brain Informer (BraInf) was developed based on an efficient self-attention mechanism. The proposed model integrates representation learning, feature distilling, and classifier modeling into a unified framework. First, the proposed model uses a multihead ProbSparse self-attention block for representation learning. This self-attention mechanism selects the first ⌊lnN⌋ elements that can represent the overall features from the perspective of probability sparsity, which significantly reduces computational cost. Subsequently, a structural distilling block is proposed that applies the concept of patch merging to the distilling operation. The block reduces the size of the three-dimensional tensor and further lowers the memory cost while preserving the original data as much as possible. Thus, there was a significant improvement in the space complexity. Finally, the feature vector was projected into the classification target space for disease prediction. The effectiveness of the proposed model was validated using the Alzheimer's Disease Neuroimaging Initiative dataset. The model achieved 97.97% and 91.89% accuracy on Alzheimer's disease and mild cognitive impairment classification tasks, respectively. The experimental results also demonstrate that the proposed framework outperforms several state-of-the-art methods.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.compbiomed.2022.105737DOI Listing
August 2022

Modulation of oxidative stress and gut microbiota by selenium-containing peptides from Cardamine enshiensis and structural-based characterization.

Food Chem 2022 Nov 21;395:133547. Epub 2022 Jun 21.

Enshi Tujia & Miao Autonomous Prefecture Academy of Agricultural Sciences, Enshi 445000, China. Electronic address:

The antioxidant properties of Se-containing peptides from Cardamine enshiensis (SeCPPs) and their impact on gut microbiota were studied in d-galactose (d-gal)- injected mice and antibiotic-treated mice. The structures of SeCPPs were identified by UPLC-Q-Extractive Orbitrap MS. In d-gal ageing mice, SeCPPs were associated with significantly decreased acetyl cholinesterase (AchE) activity, malondialdehyde (MDA) content, increased glutathione peroxidase (GSH-Px) activity, downregulated tumour necrosis factor-α (TNF-α), interleukin-6 (IL-6) and interleukin-1β (IL-1β) levels (p < 0.05), and improved memory. In antibiotic-treated mice, SeCPPs were associated with reduced Proteobacteria and evaluated Akkermansia abundances (p < 0.01). Eighty-five Se-containing peptides were identified in SeCPPs. Peptides such as RV-SeM-I, RA-SeM-T and R-SeC-K showed low binding energy with 1,1-diphenyl-2-picrylhydrazyl (DPPH), and their binding affinities were confirmed by molecular docking. Overall, compared with NaSeO and SeMet, SeCPPs showed superior antioxidant activity via their association with higher antioxidant enzyme activity, scavenging free radical properties and gut microbiome modulation.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.foodchem.2022.133547DOI Listing
November 2022

Investigation of the Disparities in Ultrasound Imaging Features of miR-323, miR-409-3p, and VEGF Expression Scales in Different Clinicopathological Features of Prostate Carcinoma and Their Correlation with Prognosis.

Biomed Res Int 2022 18;2022:5053204. Epub 2022 Jun 18.

Emergency Ultrasound Department of the First Hospital of Shanxi Medical University, Taiyuan 030001, China.

Prostate carcinoma (PC) is a disease of the male genitourinary system and a relatively common malignant tumor. In order to investigate the disparities in the expression of microRNA-323 (miR-323), microRNA-409-3p (miR-409-3p), and vascular endothelial growth factor (VEGF) in prostate carcinoma with different clinicopathological features and analyze their correlation with prognosis. Thirty-two sufferers with prostate carcinoma and forty-three sufferers with benign prostatic hyperplasia are included. The results show that the detection of miR-323, miR-409-3p, and VEGF scales can provide reference value for clinical guidance of prostate carcinoma prognosis.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1155/2022/5053204DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9233573PMC
June 2022

Amino acids imprinted ZIF-8s for the highly efficient and selective adsorption of antioxidant peptides from silkworm pupa protein.

Food Res Int 2022 Jul 26;157:111406. Epub 2022 May 26.

MOE Key Laboratory of New Processing Technology for Nonferrous Metals and Materials, Guangxi Key Laboratory of Processing for Non-ferrous Metals and Featured Materials, School of Chemistry and Chemical Engineering, Guangxi University, Nanning 530004, China; Guangxi Bossco Environmental Protection Technology Co., Ltd, Nanning 530007, China. Electronic address:

Antioxidant peptides from edible protein sources have attracted great attention due to their high activity, easy adsorption and low toxicity. The purpose of this study was to develop a new MOF material with specific recognition and capture ability to achieve the aim of highly efficient and selective antioxidant peptides from silkworm pupa protein hydrolysate-N (SPPH-N). Firstly, Histidine (His) widely presented in antioxidant peptides was used to in-suit construct the modified ZIF-8 material (ZIF-His) with imprinted defective mesopores, based on "molecular imprinting strategy". Then, it was applied to extract antioxidant peptides from SPPH-N. ZIF-His exhibited high adsorption uptake for antioxidant peptides and especially presented specific adsorption property for peptides contained His, tryptophan, phenylalanine and tyrosine. Three peptides with the highest antioxidant activity (FKVPNMY, AVNMVPFPR and VNMVPFPR) from ZIF-His eluent were identified by Nano-LC-MS/MS. These peptides were synthesized and presented the excellent free radicals scavenging activity and the Ferric reducing power.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.foodres.2022.111406DOI Listing
July 2022

Variations in Marine Bacterial and Archaeal Communities during an Green Tide in Coastal Qingdao Areas.

Microorganisms 2022 Jun 13;10(6). Epub 2022 Jun 13.

College of Marine Life Sciences, Institute of Evolution and Marine Biodiversity, Frontiers Science Center for Deep Ocean Multispheres and Earth System, Ocean University of China, Qingdao 266003, China.

Green tides caused by occur annually in the Yellow Sea, potentially influencing the marine microorganisms. Here, we focused on the variations in marine bacterial and archaeal communities during an green tide in coastal Qingdao areas with Illumina high-throughput sequencing analysis. Our results revealed that the diversity and structure of bacterial and archaeal communities, as well as the organization and structure of microbial co-occurrence networks, varied during the green tide. The decline phase may be favorable to the bacterial and archaeal diversity and richness. The bacterial community, as well as the archaeal community, showed clear variations between the outbreak and decline phases. A simpler and less connected microbial co-occurrence network was observed during the outbreak phase compared with the decline phase. and separately dominated the bacterial community during the outbreak and decline phase, and () dominated the archaeal community during the green tide. Combined with microbial co-occurrence network analysis, , and may be important organisms during the green tide. Temperature, chlorophyll content and salinity may have an important impact on the variations in bacterial and archaeal communities during the green tide.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.3390/microorganisms10061204DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9228619PMC
June 2022

Human midbrain dopaminergic neuronal differentiation markers predict cell therapy outcomes in a Parkinson's disease model.

J Clin Invest 2022 Jul;132(14)

Institute of Neuroscience, Key Laboratory of Primate Neurobiology, CAS Center for Excellence in Brain Science and Intelligence Technology, Chinese Academy of Sciences, Shanghai, China.

Human pluripotent stem cell-based (hPSC-based) replacement therapy holds great promise for the treatment of Parkinson's disease (PD). However, the heterogeneity of hPSC-derived donor cells and the low yield of midbrain dopaminergic (mDA) neurons after transplantation hinder its broad clinical application. Here, we have characterized the single-cell molecular landscape during mDA neuron differentiation. We found that this process recapitulated the development of multiple but adjacent fetal brain regions including the ventral midbrain, the isthmus, and the ventral hindbrain, resulting in a heterogenous donor cell population. We reconstructed the differentiation trajectory of the mDA lineage and identified calsyntenin 2 (CLSTN2) and protein tyrosine phosphatase receptor type O (PTPRO) as specific surface markers of mDA progenitors, which were predictive of mDA neuron differentiation and could facilitate high enrichment of mDA neurons (up to 80%) following progenitor cell sorting and transplantation. Marker-sorted progenitors exhibited higher therapeutic potency in correcting motor deficits of PD mice. Different marker-sorted grafts had a strikingly consistent cellular composition, in which mDA neurons were enriched, while off-target neuron types were mostly depleted, suggesting stable graft outcomes. Our study provides a better understanding of cellular heterogeneity during mDA neuron differentiation and establishes a strategy to generate highly purified donor cells to achieve stable and predictable therapeutic outcomes, raising the prospect of hPSC-based PD cell replacement therapies.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1172/JCI156768DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9282930PMC
July 2022

Isolation and Complete Sequence of One Novel Marine Bacteriophage PHS21 Infecting Pseudoalteromonas marina.

Curr Microbiol 2022 Jun 7;79(7):213. Epub 2022 Jun 7.

College of Marine Life Sciences, Institute of Evolution and Marine Biodiversity, Frontiers Science Center for Deep Ocean Multispheres and Earth System, Ocean University of China, Qingdao, People's Republic of China.

PHS21 against Pseudoalteromonas is isolated from Qingdao offshore seawater. The phage was characterized and identified by morphological examination, stability, whole genome sequencing, and bioinformatics analysis. Morphological analysis of PHS21 by transmission electron microscopy shows that belonged to the Siphoviridae family. PHS21 showed strong tolerance with a wide range of temperatures and pH. One-step growth assay indicates that the latent period is about 48 min and the burst size is approximately 218 PFU/cell (plaque forming unit/cell). Its complete genomic sequence is 35,802-bp long with 50 putative open reading frames. Phage PHS21 and PHS3 displayed a very close evolutionary relationship; however, having different DNA packaging proteins indicates that they may have already evolved distinct ways to package DNA in host cells. This study provides the detailed description and genomic characterization of a novel Pseudoalteromonas phage.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1007/s00284-022-02886-6DOI Listing
June 2022

Molecularly imprinted polymers outperform lectin counterparts and enable more precise cancer diagnosis.

Chem Sci 2022 Apr 17;13(16):4589-4597. Epub 2022 Mar 17.

State Key Laboratory of Analytical Chemistry for Life Science, School of Chemistry and Chemical Engineering, Nanjing University 163 Xianlin Avenue Nanjing 210023 China +86-25-8968-5639.

Accurately analysing the particular glycosylation status of protein biomarkers is of significant importance in the precise, early diagnosis of cancer. Existing methods mainly rely on the use of antibodies and lectins. However, due to the macroscopic and microscopic heterogeneity of glycans, precise analysis of glycosylation status still remains a challenge. Molecularly imprinted polymers (MIPs), as a synthetic alternative to antibodies or lectins, may provide new solutions but have not yet been explored. Herein, we report an appealing strategy called triple MIP-based plasmonic immunosandwich assay (triMIP-PISA) for precise cancer diagnosis in terms of the relative glycosylation expression of glycoprotein biomarkers. As proof of the principle, alpha fetoprotein (AFP), which has been used as a clinical biomarker for early detection of hepatocellular carcinoma (HCC), as well as its agglutinin (LCA)-reactive fraction (AFP-L3), which is mainly composed of core-fucosylated glycans, were used as two target proteoforms to test in this study. Using two MIPs that can specifically recognize the peptide sequence of AFP as well as a fucose-imprinted MIP that can specifically recognize the AFP-L3 fraction, facile simultaneous plasmon-enhanced Raman detection of AFP and AFP-L3 in serum was achieved, which allowed HCC patients to be distinguished from healthy individuals. Due to the excellent recognition properties of the MIPs that are comparable to those of antibodies and superior to those of lectins, our triMIP-PISA method exhibited improved precision as compared with an antibody plus lectin-based immunofluorescence assay. Thus, this strategy opened a new avenue towards the precise diagnosis of cancer.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1039/d2sc01093cDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9020343PMC
April 2022

Acidic microenvironment triggered assembly of activatable three-arm aptamer nanoclaw for contrast-enhanced imaging and tumor growth inhibition .

Theranostics 2022 24;12(7):3474-3487. Epub 2022 Apr 24.

State Key Laboratory of Chemo/Biosensing and Chemometrics, College of Biology, College of Chemistry and Chemical Engineering, Hunan University, Key Laboratory for Bio-Nanotechnology and Molecular Engineering of Hunan Province, Changsha 410082, China.

Rationale: Static assembled multivalent DNA nanotheranostics system have encountered some bottleneck problems in cancer imaging and therapy, such as poor penetration and high immunogenicity. Herein, we proposed an acidic tumor microenvironment triggered assembly of activatable multivalent nanodevice, called "three-arm aptamer nanoclaw" (TA-aptNC), assembled from three pH-responsive aptamer-decorated DNA monomers (pH-aptDMs) to facilitate their functions of imaging and therapy.

Methods: The activated TA-aptNC was constructed by acidic microenvironment triggered assembly of three pH-aptDMs. Designer pH-aptDM was established based on the combination of a pH-responsive i-motif switch and an assembly module with a cell membrane anchoring aptamer ligand. Acidic microenvironment-triggered the assembly of the TA-aptNC was characterized by electrophoresis and atomic force microscopic (AFM). The binding affinity and stability of the TA-aptNC, comparing the monovalent pH-aptDM, were studied via the flow cytometry and nuclease resistance assays. Acidic microenvironment-activated contrast-enhanced tumor imaging and significantly antitumor efficiency were evaluated and . : At physiological pH environment, the pH-aptDMs with excellent tissue permeability exited as inactivated and monodispersed small monomer. When encountering acidic microenvironment at the tumor site, pH-responsive i-motif switch liberated from the pH-aptDMs, and the three unconstrained DNA modules (DM, DM and DM) subsequently assembled into the TA-aptNC. Compared with monovalent pH-aptDMs, the spontaneously formed activatable TA-aptNC afforded 2-fold enhanced binding ability via the multivalent effect, which further facilitated the selective tumor cell uptake capability, thus enabling a contrast-enhanced tumor imaging and significantly antitumor efficiency without systemic toxicity. : The proposed strategy offers valuable insight into excavating an endogenous stimuli-triggered assembly of multivalent nanodevice for accurate diagnosis and efficient tumor therapy.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.7150/thno.72028DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9065194PMC
April 2022

Oxidized high-density lipoprotein promotes CD36 palmitoylation and increases lipid uptake in macrophages.

J Biol Chem 2022 06 29;298(6):102000. Epub 2022 Apr 29.

Department of Biochemistry and Molecular Biology, School of Basic Medical Science, Guizhou Medical University, Guiyang, Guizhou, China. Electronic address:

Oxidized high-density lipoprotein (oxHDL) reduces the ability of cells to mediate reverse cholesterol transport and also shows atherogenic properties. Palmitoylation of cluster of differentiation 36 (CD36), an important receptor mediating lipoprotein uptake, is required for fatty acid endocytosis. However, the relationship between oxHDL and CD36 has not been described in mechanistic detail. Here, we demonstrate using acyl-biotin exchange analysis that oxHDL activates CD36 by increasing CD36 palmitoylation, which promotes efficient uptake in macrophages. This modification increased CD36 incorporation into plasma lipid rafts and activated downstream signaling mediators, such as Lyn, Fyn, and c-Jun N-terminal kinase, which elicited enhanced oxHDL uptake and foam cell formation. Furthermore, blocking CD36 palmitoylation with the pharmacological inhibitor 2-bromopalmitate decreased cell surface translocation and lowered oxHDL uptake in oxHDL-treated macrophages. We verified these results by transfecting oxHDL-induced macrophages with vectors expressing wildtype or mutant CD36 (mCD36) in which the cytoplasmic palmitoylated cysteine residues were replaced. We show that cells containing mCD36 exhibited less palmitoylated CD36, disrupted plasma membrane trafficking, and reduced protein stability. Moreover, in ApoECD36 mice, lipid accumulation at the aortic root in mice receiving the mCD36 vector was decreased, suggesting that CD36 palmitoylation is responsible for lipid uptake in vivo. Finally, our data indicated that palmitoylation of CD36 was dependent on DHHC6 (Asp-His-His-Cys) acyltransferase and its cofactor selenoprotein K, which increased the CD36/caveolin-1 interaction and membrane targeting in cells exposed to oxHDL. Altogether, our study uncovers a causal link between oxHDL and CD36 palmitoylation and provides insight into foam cell formation and atherogenesis.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.jbc.2022.102000DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9144050PMC
June 2022

A comparative study on the coordination of diglycolamide isomers with Nd(iii): extraction, third phase formation, structure, and computational studies.

RSC Adv 2021 Aug 19;11(45):27969-27977. Epub 2021 Aug 19.

School of Chemistry and Chemical Engineering, Key Laboratory of Cluster Science of Ministry of Education, Beijing Institute of Technology Beijing 100081 China.

A novel asymmetric diglycolamide ,-dimethyl-','-dioctyl diglycolamide (L) was synthesized. The Nd(iii) extraction behavior from HNO and loading capability of the solution of L in 40/60 (v/v)% -octanol/kerosene were studied. Analyses by the slope method, ESI-MS, and FT-IR indicated that, similar to the previously studied isomer ligand ,'-dimethyl-,'-dioctyl diglycolamide (L), 1 : 3 Nd(iii)/L complexes formed. Under the same experimental conditions, the distribution ratio and limiting organic concentration of L towards Nd(iii) were smaller than those of L, but the critical aqueous concentration of L was larger, which implies that L exhibited poorer extraction and loading capabilities towards Nd(iii) than L, and L has a tendency to be less likely to form the third phase. The quasi-relativistic density functional theory (DFT) calculation was performed to provide some explanations for the differences in their extraction behaviors. The electrostatic potential of the ligands indicated that the electron-donating ability of the amide O atoms in L displayed certain differences compared with L. This inhomogeneity in L affected the interaction between L and Nd(iii), as supported by QTAIM and bonding nature analysis, and it seemed to reflect in the extraction performance towards Nd(iii).
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1039/d1ra04222jDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9037995PMC
August 2021

Synthesis of luminescent thorium-based metal-organic frameworks with 1,2,4,5-tetrakis(4-carboxyphenyl)benzene.

RSC Adv 2021 May 12;11(28):17431-17436. Epub 2021 May 12.

China Institute of Atomic Energy, Department of Radiochemistry Beijing 102413 China

Three new thorium-based MOFs based on 1,2,4,5-tetrakis(4-carboxyphenyl)benzene (H4TCPB) were obtained under a similar reaction system (metal salt, ligand, solvent, and acid are the same). Th(iv) forms the central unit of the MOFs in mononuclear and binuclear clusters, respectively. All the MOFs show blue ligand-based luminescence under an ultraviolet environment. This is the first time that multiple thorium-based MOFs with luminescence have been found with the same ligand.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1039/d1ra01742jDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9033178PMC
May 2021

The Added Value of Virtual Unenhanced Images Obtained From Dual-energy CT Urography in the Detection and Measurement of Urinary Stone.

Urology 2022 Aug 22;166:118-125. Epub 2022 Apr 22.

Department of Radiology, the First Affiliated Hospital of Xi'an Jiaotong University, Xi'an, Shaanxi province, PR China. Electronic address:

Objective: To evaluate the detection and quantification of urinary stones using virtual unenhanced images (VUE) at different phases and slice thickness in contrast-enhanced dual-energy CT Urography (DECTU) in comparison with true unenhanced images (TUE).

Methods: One hundred and twelve urinary stone patients who required triphasic DECTU were analyzed. Data were reconstructed as the followings: TUE images with 1.25 mm thickness (TUE portal venous phase VUE images with 1.25 and 5-mm thickness (VUE(VP)_1.25 mm and VUE(VP)_5 mm) and excretory phase VUE images with 1.25 and 5-mm thickness (VUE(EP)_1.25mm and VUE(EP)_5mm). The. The stones were divided into large (≥5 mm) and small stones. The detection rate, size and CT value of stones were assessed by 2 radiologists and statistically compared among the above groups.

Results: Two hundred and thirty urinary stones (163 large and 67 small stones) were detected on TUE_1.25 mm images. For large stones, the detection rate on VUE(VP)_1.25 mm, VUE(VP)_5 mm, VUE(EP)_1.25 mm and VUE(EP)_5 mm was 100%, 96.9%, 85.9%, and 80.4%; while for small stones, the rate was 77.6%, 37.3%, 46.3%, and 23.9%, respectively. VUE(VP) images significantly improved the stone detection rate compared with VUE(EP) images at both slice thicknesses. In general, VUE images identified stones with smaller sizes and lower Hounsfield units, but thinner slice thickness images reduced the inaccuracy. Inter-reader agreement of the stone detection revealed a k value range from 0.85 to 0.94 for TUE and VUE images.

Conclusion: Large stones (≥5 mm) can reliably be detected on thin section VUE(VP) images with 33% radiation dose reduction. However, for small stones TUE remains superior. Stone size is underestimated on VUE images.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.urology.2022.02.029DOI Listing
August 2022

Screening and bioavailability evaluation of anti-oxidative selenium-containing peptides from soybeans based on specific structures.

Food Funct 2022 May 10;13(9):5252-5261. Epub 2022 May 10.

College of Food Science and Technology, Huazhong Agricultural University, Wuhan 430070, China.

Our previous study has evaluated the antioxidant capacity and identified the sequences of soybean selenium-containing peptides. Herein, pharmacophore screening, gastrointestinal simulation and pharmacokinetics were performed to predict the potentials of selenium-containing peptides in terms of antioxidant activity, safety and bioavailability. A pharmacophore model with 6 structure features was constructed for virtual screening to determine the potential activities of 85 selenium sequences from soybean peptides. Strong reversing effects ( < 0.05) of the targeted sequences were observed in tumor necrosis factor-α (TNF-α)-induced inflammatory cytokines and adhesion factors burst in EA·hy926/Caco-2 co-culture cell models. Ser-Phe-Gln-SeMet (SFQSeM), a promising peptide selected from both virtual screening and cell models, was proved to be stable in the gastrointestinal tract and could be transported across the Caco-2 monolayer the paracellular pathway. Additionally, SFQSeM showed a long residence time (89.42 ± 1.34 min) and half-life (81.60 ± 11.88 min) after consumption, and it induced lower liver alanine/aspartate transaminase (ALT/AST) and serum nitric oxide (NO) levels compared to NaSeO and SeMet ( < 0.05). The potency of SFQSeM against oxidative stress as well as its oral bioavailability and low risk highlight its potential utility as an effective Se nutritional supplement.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1039/d2fo00113fDOI Listing
May 2022

MiR-130a-3p regulates neural stem cell differentiation in vitro by targeting Acsl4.

J Cell Mol Med 2022 05 16;26(9):2717-2727. Epub 2022 Apr 16.

Department of Human Anatomy, Institute of Neurobiology, Nantong University, Nantong, China.

In the adult mammalian brain, neural stem cells (NSCs) are the precursor cells of neurons that contribute to nervous system development, regeneration, and repair. MicroRNAs (miRNAs) are small non-coding RNAs that regulate cell fate determination and differentiation by negatively regulating gene expression. Here, we identified a post-transcriptional mechanism, centred around miR-130a-3p that regulated NSC differentiation. Importantly, overexpressing miR-130a-3p promoted NSC differentiation into neurons, whereas inhibiting miR-130a-3p function reduced the number of neurons. Then, the quantitative PCR, Western blot and dual-luciferase reporter assays showed that miR-130a-3p negatively regulated acyl-CoA synthetase long-chain family member 4 (Acsl4) expression. Additionally, inhibition of Acsl4 promoted NSC differentiation into neurons, whereas silencing miR-130a-3p partially suppressed the neuronal differentiation induced by inhibiting Acsl4. Furthermore, overexpressing miR-130a-3p or inhibiting Acsl4 increased the levels of p-AKT, p-GSK-3β and PI3K. In conclusion, our results suggested that miR-130a-3p targeted Acsl4 to promote neuronal differentiation of NSCs via regulating the Akt/PI3K pathway. These findings may help to develop strategies for stem cell-mediated treatment for central nervous system diseases.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1111/jcmm.17285DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9077303PMC
May 2022

Study on the extraction of lanthanides by isomeric diglycolamide extractants: an experimental and theoretical study.

RSC Adv 2021 Dec 24;12(2):790-797. Epub 2021 Dec 24.

School of Chemistry and Chemical Engineering, Key Laboratory of Cluster Science of Ministry of Education, Beijing Institute of Technology Beijing 100081 China.

Two isomeric diglycolamide (DGA) extractants, ,'-dimethyl-,'-dioctyl diglycolamide (L) and ,-dimethyl-','-dioctyl diglycolamide (L), were used to perform a comparative study on the extraction performances towards several lanthanides by extraction experiments and theoretical calculations. The experimental results show that both L and L show a positive sequence on the extraction of lanthanides, and L exhibits the higher complex ability with these lanthanides than L, except for La and Ce. Slope analysis shows that 1:2 or 1:3 complexes are formed between the two ligands and the metal ions. The geometrical structures of the complexes were optimized in the gas phase by density functional theory (DFT) on the basis of complex compositions. The results of bond lengths, MBOs and topological analysis indicated that the electrostatic interaction between metal ions and two amide O atoms in the L ligand is not as homogeneous as in L, and this inhomogeneity is likely to be related to the poor extraction performance of L.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1039/d1ra07020gDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8978646PMC
December 2021

The Influence of Performance-Contingent Rewards on Proactive and Responsive Creativity: Dual-Path Mediating Effects of Work Motivation.

Front Psychol 2022 11;13:812298. Epub 2022 Mar 11.

Business School, Beijing Technology and Business University, Beijing, China.

Creativity has become prevalent in the routine work of knowledge employees in contemporary enterprises. From the perspective of ambidexterity, drawing upon expectancy theory and self-determination theory (SDT), the present study highlights the driver behind proactive and responsive creativity. Using two-stage longitudinal data collected from 373 knowledge employee-supervisor dyads in information and manufacturing companies in China, the results show that: (1) performance-contingent rewards have an inverted U-shaped influence on proactive creativity and a U-shaped influence on responsive creativity; (2) performance-contingent rewards have an inverted U-shaped influence on autonomous and controlled motivation; (3) autonomous motivation has a positive influence on proactive creativity, but controlled motivation has a negative influence on responsive creativity; (4) autonomous and controlled motivation play a partly mediating mechanism in the non-linear effects of performance-contingent rewards on proactive and responsive creativity, respectively. Managers should enhance the ambidextrous ability to deal with proactive and responsive creativity and establish an applied and dynamic policy of performance-contingent rewards intensity to drive ambidextrous creativity.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.3389/fpsyg.2022.812298DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8962671PMC
March 2022

TPX2 Serves as a Cancer Susceptibility Gene and Is Closely Associated with the Poor Prognosis of Endometrial Cancer.

Genet Res (Camb) 2022 16;2022:5401106. Epub 2022 Mar 16.

Clinical Laboratory Department, Benxi Central Hospital of China Medical University, Benxi, Liaoning 117022, China.

Background: Endometrial cancer (EC) is a common tumor of the genital tract that affects the female reproductive system but with only limited treatment options. We aimed to discover new prognostic biomarkers for EC.

Methods: We used mRNA-seq data to detect differentially expressed genes (DEGs) between EC and control tissues. Detailed clinicopathological information was collected, and changes in the mRNA and protein levels of hub DEGs were analyzed in EC. Copy number variation (CNV) was also evaluated for its association with the pathogenesis of EC. Gene set enrichment analysis (GSEA) was conducted to enrich significant pathways driven by the hub genes. Cox regression analysis was used to select variables to create a nomogram. The nomogram was calibrated by applying the concordance index (C-index), and net benefits of the nomogram at different threshold probabilities were quantified using decision curve analysis (DCA).

Results: Differential expression analysis identified 24 DEGs as potential risk factors for EC. Survival analysis revealed that TPX2 expression was related to worsening overall survival in patients with advanced EC. A high CNV was associated with the overexpression of TPX2; this suggested that modifications in the cell-cycle pathway might be crucial in the advancement of EC. Moreover, an individualized nomogram was developed for TPX2 incorporating clinical factors; this was also evaluated for its ability to predict EC. Calibration and DCA analyses confirmed the robustness and clinical usefulness of the nomogram.

Conclusion: We offer novel insights into the pathogenesis and molecular mechanisms of EC. The overexpression of TPX2 was related to a poorer prognosis and could serve as a biomarker for predicting prognostic outcomes in EC patients.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1155/2022/5401106DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8942693PMC
March 2022
-->