Publications by authors named "Hui Han"

490 Publications

High Expression of PKM2 Was Associated with the Poor Prognosis of Acute Leukemia.

Cancer Manag Res 2021 13;13:7851-7858. Epub 2021 Oct 13.

Department of Laboratory Medicine, Sun Yat-sen University Affiliated Zhongshan Hospital, Zhongshan, Guangdong Province, People's Republic of China.

Purpose: To explore the clinical significance of plasma pyruvate kinase M2 (PKM2) in assessing the incidence and prognosis of acute leukemia.

Methods: Plasma samples from 56 acute myeloid leukemia (AML) patients, 40 acute lymphoblastic leukemia (ALL) patients, and 66 plasma samples from healthy individuals were collected. The level of plasma PKM2 was detected by enzyme-linked immunosorbent assay. The clinical significance of PKM2 in acute leukemia was assessed by analyzing receiver operating characteristic and survival curves.

Results: The plasma levels of PKM2 in AML or ALL patients were significantly higher than those in healthy individuals, respectively. PKM2 can be used as a potential diagnostic index with the AUC of 0.827 for AML and 0.837 for ALL. The level of plasma PKM2 in ALL patients with a BCR/ABL-positive genotype was significantly higher than that in patients with a BCR/ABL-negative genotype (p<0.05). The event-free survival and the overall survival of acute leukemia patients with higher PKM2 expression was worse than those with lower PKM2 expression.

Conclusion: This study showed that higher levels of PKM2 was negatively correlated with the prognosis of acute leukemia. Therefore, PKM2 can be used as a potential index to assess the incidence and prognosis of acute leukemia.
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http://dx.doi.org/10.2147/CMAR.S331076DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8520821PMC
October 2021

Dihydroquercetin supplement alleviates colonic inflammation potentially through improved gut microbiota community in mice.

Food Funct 2021 Oct 21. Epub 2021 Oct 21.

State Key Laboratory of Animal Nutrition, Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing 100193, China.

The purpose of the current study was to investigate the effect of dietary dihydroquercetin (DHQ) supplementation on dextran sodium sulfate (DSS)-induced colitis in mice. Mice were given DHQ supplementation (3 g kg) throughout the study, starting 14 days prior to DSS treatment for 1 week followed by 2 days without DSS. The results showed that dietary DHQ supplementation restored DSS-induced disease activity index (DAI), colon length and histopathology scores of the colon tissue. Additionally, supplementation with DHQ reduced the pro-inflammatory cytokine levels, and enhanced the level of IL-10 in the serum. qPCR results indicated that DHQ supplementation significantly downregulated , , and , and upregulated gene mRNA expression. Western blot results proved that DHQ supplementation upregulated ZO-1 and occludin levels. Using amplicon sequencing technology, 16S rRNA sequencing results showed that DHQ supplementation increased the fecal / ratio and the relative abundance of and , and decreased the relative abundance of . Additionally, DHQ supplementation restored the decreased fecal acetic acid and butyric acid concentrations in DSS-induced colitis mice. Besides, Spearman's correlation analysis showed that was positively correlated with the butyric acid level and was positively correlated with the mRNA expression of and . Both and showed a negative correlation with the mRNA expression of , , and , and was positively correlated with . In summary, it was found that DHQ supplementation alleviated DSS-induced colitis which may be potentially associated with altered fecal microbiota communities in mice.
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http://dx.doi.org/10.1039/d1fo01422fDOI Listing
October 2021

Aerenchyma formation in the root of leaf-vegetable sweet potato: programmed cell death initiated by ethylene-mediated H O accumulation.

Physiol Plant 2021 Oct 21. Epub 2021 Oct 21.

Research Center of Crop Stresses Resistance Technologies/ Engineering Research Centre of Ecology and Agricultural Use of Wetland, Ministry of Education, Yangtze University, Jingzhou, China.

Sweet potato, commonly planted in Southeast Asia and South America with abundant rainfall, often suffers from waterlogging. The aerenchyma formation in roots is an effective way for plants to facilitate gas exchange. In the present study, tolerant and sensitive varieties, respectively designated NC1 and C211, were evaluated under water oxygen content at 2.0 mg·L (hypoxia treatment) and 8.0 mg·L (control). The results showed that NC1 variety has a relatively higher root growth rate under low oxygen condition. In NC1 plants, aerenchyma was observed in the mid-section of the main adventitious root and spread to the proximal and distal ends, forming a complete channel in the cortex. However, in C211 plants, the aerenchyma occurred relatively later and could not turn into a whole channel. Ethylene synthesis (ACS1, ACS4, ACS5, etc.) and signal transduction (ETR1, ERS1, EIN2, etc.) related genes were upregulated in the NC1 plants and led to changes in the reactive oxygen species (ROS) related genes (RBOHA, SOD, CAT, etc.) and enzyme activities. It was found that programmed cell death (PCD) was induced by H O accumulation. A regulatory model of lysigenous aerenchyma formation in the root of sweet potato was constructed. Our study enriches the understanding of the mechanisms of the aerenchyma formation in plants. This article is protected by copyright. All rights reserved.
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http://dx.doi.org/10.1111/ppl.13587DOI Listing
October 2021

λ-carrageenan exacerbates Citrobacter rodentium-induced infectious colitis in mice by targeting gut microbiota and intestinal barrier integrity.

Pharmacol Res 2021 Oct 16;174:105940. Epub 2021 Oct 16.

Department of Laboratory Medicine, Ningbo Medical Center Lihuili Hospital, Ningbo University, Ningbo 315040, China. Electronic address:

For nearly half a century, the scientific community has been unable to agree upon the safety profile of carrageenan (CGN), a ubiquitous food additive. Little is known about the mechanisms by which consumption of CGN aggravates the etiopathogenesis of murine colitis. However, analyses of gut microbiota and intestinal barrier integrity have provided a breakthrough in explaining the synergistic effect of CGN upon colitis. In Citrobacter rodentium-induced infectious murine colitis, inflammation and the clinical severity of gut tissue were aggravated in the presence of λ-CGN. Using fecal transplantation and germ-free mice experiments, we evaluated the role of intestinal microbiota on the pro-inflammatory effect of λ-CGN. Mice with high dietary λ-CGN consumption showed altered colonic microbiota composition that resulted in degradation of the colonic mucus layer, a raised fecal LPS level, and a decrease in the presence of bacterially derived short-chain fatty acids (SCFAs). Mucus layer defects and altered fecal LPS and SCFA levels could be reproduced in germ-free mice by fecal transplantation from CGN-H-fed mice, but not from germ-free CGN-H-fed mice. Our results confirm that λ-CGN may create an environment that favors inflammation by altering gut microbiota composition and gut bacterial metabolism. The present study provides evidence that the "gut microbiota-barrier axis" could be an alternative target for ameliorating the colitis promoting effect of λ-CGN.
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http://dx.doi.org/10.1016/j.phrs.2021.105940DOI Listing
October 2021

Butyric and Citric Acids and Their Salts in Poultry Nutrition: Effects on Gut Health and Intestinal Microbiota.

Int J Mol Sci 2021 Sep 27;22(19). Epub 2021 Sep 27.

State Key Laboratory of Animal Nutrition, Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing 100193, China.

Intestinal dysfunction of farm animals, such as intestinal inflammation and altered gut microbiota, is the critical problem affecting animal welfare, performance and farm profitability. China has prohibited the use of antibiotics to improve feed efficiency and growth performance for farm animals, including poultry, in 2020. With the advantages of maintaining gut homeostasis, enhancing digestion, and absorption and modulating gut microbiota, organic acids are regarded as promising antibiotic alternatives. Butyric and citric acids as presentative organic acids positively impact growth performance, welfare, and intestinal health of livestock mainly by reducing pathogenic bacteria and maintaining the gastrointestinal tract (GIT) pH. This review summarizes the discovery of butyric acid (BA), citric acid (CA) and their salt forms, molecular structure and properties, metabolism, biological functions and their applications in poultry nutrition. The research findings about BA, CA and their salts on rats, pigs and humans are also briefly reviewed. Therefore, this review will fill the knowledge gaps of the scientific community and may be of great interest for poultry nutritionists, researchers and feed manufacturers about these two weak organic acids and their effects on intestinal health and gut microbiota community, with the hope of providing safe, healthy and nutrient-rich poultry products to consumers.
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http://dx.doi.org/10.3390/ijms221910392DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8508690PMC
September 2021

Circular RNA circSNX6 promotes sunitinib resistance in renal cell carcinoma through the miR-1184/GPCPD1/ lysophosphatidic acid axis.

Cancer Lett 2021 Oct 7;523:121-134. Epub 2021 Oct 7.

Department of Oncology, First Affiliated Hospital, Sun Yat-sen University, Guangzhou, China. Electronic address:

Sunitinib resistance is a major challenge in systemic therapy for renal cell carcinoma (RCC). The role of circular RNAs (circRNAs) in regulating sunitinib resistance of RCC is largely unknown. We established sunitinib-resistant RCC cell lines in vivo. Through RNA-sequencing, we identified circSNX6, whose expression is upregulated in sunitinib-resistant cells compared with their parental cells. High circSNX6 expression was correlated with sunitinib resistance and worse oncologic outcomes in a cohort of 81 RCC patients. In vitro and in vivo experiments confirmed that circSNX6 could promote sunitinib resistance in RCC. circSNX6 acts as a molecular "sponge" to relieve the suppressive effect of microRNA (miR)-1184 on its target gene, glycerophosphocholine phosphodiesterase 1 (GPCPD1), which increases intracellular lysophosphatidic acid (LPA) levels and, ultimately, promotes sunitinib resistance in RCC cells. Our findings demonstrated that the circSNX6/miR-1184/GPCPD1 axis had a critical role in regulation of intracellular LPA levels and sunitinib resistance in RCC; they also provide a novel prognostic indicator and promising therapeutic targets.
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http://dx.doi.org/10.1016/j.canlet.2021.10.003DOI Listing
October 2021

Three new species of the genus Hampson, 1893 (Lepidoptera, Erebidae, Boletobiinae) from the Xizang Autonomous Region, China with an updated list of the world species.

Zookeys 2021 15;1060:17-32. Epub 2021 Sep 15.

Laboratory of Entomology, Federal Scientific Center of the East Asia Terrestrial Biodiversity, Far Eastern Branch, Russian Academy of Sciences, Vladivostok-22, 690022, Russia.

Three new species of the genus Hampson, 1893: , and are described from Motuo (= Medog) County of the Xizang Autonomous Region (= Tibet), China. The imagines as well as the male genitalia are illustrated. A checklist of the 45 species of the genus in the world fauna is presented, including recently and presently described species.
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http://dx.doi.org/10.3897/zookeys.1060.67674DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8458265PMC
September 2021

Deep Inguinal Lymph Node Metastases Can Predict Pelvic Lymph Node Metastases and Prognosis in Penile Squamous Cell Carcinoma.

Front Oncol 2021 15;11:715799. Epub 2021 Sep 15.

Department of Urology, Sun Yat-sen University Cancer Center, Guangzhou, China.

Objectives: To evaluate the relationship between deep inguinal lymph node metastasis (ILNM) and pelvic lymph node metastasis (PLNM) and explore the prognostic value of deep ILNM in penile squamous cell carcinoma (PSCC).

Materials And Methods: The records of 189 patients with ILNM treated for PSCC were analysed retrospectively. Logistic regression models were used to test for predictors of PLNM. Cox regression was performed in univariable and multivariable analyses of cancer-specific survival (CSS). CSS was compared using Kaplan-Meier analyses and log rank tests.

Results: PLNM were observed in 53 cases (28.0%). According to logistic regression models, only deep ILNM (OR 9.72, p<0.001) and number (≥3) of metastatic inguinal lymph nodes (ILNs) (OR 2.36, p=0.03) were independent predictors of PLNM. The incidences of PLNM were 18% and 19% with negative deep ILNM and extranodal extension (ENE); and 76% and 42% with positive deep ILNM and ENE, respectively. The accuracy of deep ILNM, ENE, bilateral involvement and number (≥3) of ILNMs for predicting PLNM were 81.0%, 65.6%, 63.5% and 67.2%, respectively. The CSS was significantly different in patients with positive and negative deep ILNM (median 1.7 years not reached, p<0.01). Patients who presented with deep ILNM had worse CSS (median 3.8 years not reached, p<0.01) in those with negative PLNs.

Conclusions: Deep ILNM is the most accurate factor for predicting PLNM in PSCC according to our data. We recommend that patients with deep ILNM should be referred for pelvic lymph node dissection. Involvement of deep ILNs indicates poor prognosis. We propose that patients with metastases of deep ILNs may be staged as pN3.
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http://dx.doi.org/10.3389/fonc.2021.715799DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8479104PMC
September 2021

Prevalence of Falls Among the Rural Elderly - Three PLADs of Western China, 2017-2018.

Authors:
Tong Xu Hui Han

China CDC Wkly 2020 Nov;2(46):877-880

National Center for Women and Children's Health, Chinese Center for Disease Control and Prevention, Beijing, China.

What Is Already Known About This Topic?: As population aging becomes serious in China, the elderly health problems stand out prominently. Prevention of falls of the elderly has become an important subject in China's public health.

What Is Added By This Report?: The prevalence of falls among rural elderly in western China was 9.6%. The highest prevalence was registered among the groups of female, aged 70 and over, or Salar ethnicities, or with visual deficiency and chronic diseases, of which 33.0% fell subjectively due to their poor body balance, and 65.8% fell objectively due to slippery floor or ground obstacles.

What Are The Implications For Public Health Practice?: Considering the health status of the elderly in the western China and the prevalent fall-related risk factors, health education in respect of falls prevention should be performed. Moreover, the home environment of the elderly should be checked for potential safety hazards and improved if necessary, and medical and health resources should be rationally allocated to target population in order to avoid any reoccurrence of falls injury and thus relieving the burdens upon individuals, families and the society.
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http://dx.doi.org/10.46234/ccdcw2020.239DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8393126PMC
November 2020

Two-Week Prevalence of Disease Among the Rural Elderly - 6 Provinces, China, 2018-2019.

Authors:
Tong Xu Hui Han

China CDC Wkly 2020 Sep;2(38):736-739

National Center for Women and Children's Health, Chinese Center for Disease Control and Prevention, Beijing, China.

As population aging becomes serious in China, the elderly health problems stand out prominently. The 2-week prevalence of the elderly is rising year by year, but it has been rarely studied for the rural seniors in the central and western China.



The 2-week prevalence rate of rural elderly in the central and western China is 28.5%, and it varies among different ethnic groups. The top prevalence is chronic diseases, and the severity of diseases is higher in female and high age group (80-year-old and over) people.



Considering the health status, health awareness and ethnic differences of the elderly in the central and western China, medical and health resources should be rationally allocated to prevent and treat chronic diseases and support differentiated health services. This is of great significance for the development of health service plan.
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http://dx.doi.org/10.46234/ccdcw2020.184DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8422219PMC
September 2020

High-Dose versus Standard-Dose Tigecycline Treatment of Secondary Bloodstream Infections Caused by Extensively Drug-Resistant : An Observational Cohort Study.

Infect Drug Resist 2021 18;14:3837-3848. Epub 2021 Sep 18.

Shandong Provincial Clinical Research Center for Emergency and Critical Care Medicine, Institute of Emergency and Critical Care Medicine of Shandong University, Chest Pain Center, Qilu Hospital of Shandong University, Jinan, People's Republic of China.

Background: Extensively drug-resistant (XDR-AB) infections have become difficult to treat and are associated with a high mortality rate. Tigecycline is one of the most effective agents used to treat XDR-AB infections, but data from treating bloodstream infection (BSI) in standard dose do not look promising, because of its low plasma concentration. Secondary BSI with primary infection source may indicate tigecycline treatment with a higher dose. Currently, little is known about the application of high-dose tigecycline among patients with secondary BSI caused by XDR-AB. We aimed to investigate the outcomes for high-dose (HD) tigecycline treatment versus standard-dose (SD) treatment of these patients.

Methods: An observational cohort study was conducted at four university affiliated hospitals in mainland China. Adult inpatients who were confirmed as having secondary BSI caused by XDR-AB and received definitive tigecycline treatment were consecutively included. Patients who were treated with 50 mg every 12 h were defined as the SD group, and a twice dose was defined as the HD group.

Results: Of the enrolled patients, 63 received SD and 88 received HD tigecycline treatment. Patients in the two groups had similar with regard to baseline clinical conditions. The 30-day survival was affected by the source of the primary infection. Survival was significantly better in patients with non-pulmonary-infection-related BSI than in patients with pulmonary-infection-related BSI. Multivariate Cox regression confirmed that HD had a protective effect only observed in patients with non-pneumonia-related BSI.

Conclusion: A tigecycline dose that is twice its standard dose is better for the treatment of XDR-AB infection only in BSI associated with non-pulmonary infection.
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http://dx.doi.org/10.2147/IDR.S322803DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8457649PMC
September 2021

The Integrated "Multiomics" Landscape at Peak Injury and Resolution From Alcohol-Associated Liver Disease.

Hepatol Commun 2021 Aug 28. Epub 2021 Aug 28.

Department of Pathology, University of Illinois at Chicago, Chicago, IL, USA.

Alcohol-associated liver disease (ALD) is a significant clinical problem for which the most effective therapy is alcohol abstinence. The two aims of this study were, first, to identify the liver transcriptome, fecal microbiome, and portal serum metabolome at peak injury and during early and late resolution from ALD; and second, to integrate their interactions and understand better the pathogenesis of ALD. To provoke alcohol-induced liver injury, female and male wild-type mice were fed the control or ethanol Lieber-DeCarli diets for 6 weeks. To study early and late resolution, alcohol was withdrawn from the diet and mice were sacrificed after 3 and 14 days, respectively. At peak injury, there was increased signal transducer and activator of transcription (Stat3), Rho-GTPases, Tec kinase and glycoprotein VI (Gp6), and decreased peroxisome proliferator-activated receptor signaling. During resolution from ALD, there was up-regulation of vitamin D receptor/retinoid X receptor, toll-like receptor, p38 and Stat3, and down-regulation of liver X receptor signaling. Females showed significant changes in catabolic pathways, whereas males increased cellular stress, injury, and immune-response pathways that decreased during resolution. The bacterial genus Alistipes and the metabolite dipeptide glycyl-L-leucine increased at peak but decreased during resolution from ALD in both genders. Hepatic induction of mitogen-activated protein kinase (Map3k1) correlated with changes in the microbiome and metabolome at peak but was restored during ALD resolution. Inhibition of MAP3K1 protected from ALD in mice. Conclusion: Alcohol abstinence restores the liver transcriptome, fecal microbiome, and portal serum metabolome in a gender-specific manner. Integration of multiomics data identified Map3k1 as a key gene driving pathogenesis and resolution from ALD.
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http://dx.doi.org/10.1002/hep4.1793DOI Listing
August 2021

Single-Cell Transcriptome Sequencing and Proteomics Reveal Neonatal Ileum Dynamic Developmental Potentials.

mSystems 2021 Sep 21:e0072521. Epub 2021 Sep 21.

State Key Laboratory of Animal Nutrition, Institute of Animal Sciences, Chinese Academy of Agricultural Sciences, Beijing, People's Republic of China.

The neonatal period is a crucial time during development of the mammalian small intestine. Moreover, neonatal development and maturation of the small intestine are exceptionally important for early growth, successful weaning, and postweaning growth and development, in order to achieve species-specific milestones. Although several publications recently characterized intestinal epithelial cell diversity at the single-cell level, it remains unclear how differentiation and molecular interactions take place between types and subtypes of epithelial cells during the neonatal period. A single-cell RNA sequencing (scRNA-seq) survey of 40,186 ileal epithelial cells and proteomics analysis of ileal samples at 6 time points in the swine neonatal period were performed. The results revealed previously unknown developmental changes: specific increases in undifferentiated cells, unique enterocyte differentiation, and time-dependent reduction in secretory cells. Moreover, we observed specific transcriptional factors, ligand-receptor pairs, G protein-coupled receptors, transforming growth factor β, bone morphogenetic protein signaling pathways, and gut mucosal microbiota playing vital roles in ileal development during the neonatal window. This work offers new comprehensive information regarding ileal development throughout the neonatal period. Reference to this data set may assist in the creation of novel interventions for inflammation-, metabolism-, and proliferation-related gut pathologies. We found previously unknown neonatal ileum developmental potentials: specific increases in undifferentiated cells, unique enterocyte differentiation, and time dependent reduction in secretory cells. Specific transcriptional factors (TFs), ligand-receptor pairs, G protein-coupled receptors, transforming growth factor β, bone morphogenetic protein signaling pathways, and the gut mucosal microbiota are involved in this process. Our results may assist in the creation of novel interventions for inflammation-, metabolism-, and proliferation-related gut pathologies.
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http://dx.doi.org/10.1128/mSystems.00725-21DOI Listing
September 2021

METTL3-mediated mA mRNA modification promotes esophageal cancer initiation and progression via Notch signaling pathway.

Mol Ther Nucleic Acids 2021 Dec 21;26:333-346. Epub 2021 Jul 21.

Center for Translational Medicine, Precision Medicine Institute, The First Affiliated Hospital, Sun Yat-sen University, Guangzhou 510080, China.

Esophageal cancer is a lethal malignancy with a high mortality rate, while the molecular mechanisms underlying esophageal cancer pathogenesis are still poorly understood. Here, we found that the N6-methyladenosine (mA) methyltransferase-like 3 (METTL3) is significantly upregulated in esophageal squamous cell carcinoma (ESCC) and associated with poor patient prognosis. Depletion of METTL3 results in decreased ESCC growth and progression and . We further established ESCC initiation and progression models using conditional knockout mouse and revealed that 3METTL3-mediated mA modification promotes ESCC initiation and progression . Moreover, using METTL3 overexpression ESCC cell model and conditional knockin mouse model, we demonstrated the critical function of METTL3 in promoting ESCC tumorigenesis and . Mechanistically, METTL3-catalyzed mA modification promotes NOTCH1 expression and the activation of the Notch signaling pathway. Forced activation of Notch signaling pathway successfully rescues the growth, migration, and invasion capacities of METTL3-depleted ESCC cells. Our data uncovered important mechanistical insights underlying ESCC tumorigenesis and provided molecular basis for the development of novel strategies for ESCC diagnosis and treatment.
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http://dx.doi.org/10.1016/j.omtn.2021.07.007DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8416973PMC
December 2021

The Matrisome Genes From Hepatitis B-Related Hepatocellular Carcinoma Unveiled.

Hepatol Commun 2021 Sep 16;5(9):1571-1585. Epub 2021 Jun 16.

Department of Pathology, University of Illinois at Chicago, Chicago, IL, USA.

Chronic hepatitis B virus (HBV) infection changes the composition of the extracellular matrix (ECM) and enables the onset and progression of hepatocellular carcinoma (HCC). The ensemble of ECM proteins and associated factors is a major component of the tumor microenvironment. Our aim was to unveil the matrisome genes from HBV-related HCC. Transcriptomic and clinical profiles from 444 patients with HBV-related HCC were retrieved from the Gene Expression Omnibus (GEO) and Cancer Genome Atlas (TCGA) repositories. Matrisome genes associated with HBV-related hepatocarcinogenesis, matrisome gene modules, HCC subgroups, and liver-specific matrisome genes were systematically analyzed, followed by identification of their biological function and clinical relevance. Eighty matrisome genes, functionally enriched in immune response, ECM remodeling, or cancer-related pathways, were identified as associated with HBV-related HCC, which could robustly discriminate HBV-related HCC tumor from nontumor samples. Subsequently, four significant matrisome gene modules were identified as showing functional homogeneity linked to cell cycle activity. Two subgroups of patients with HBV-related HCC were classified based on the highly correlated matrisome genes. The high-expression subgroup (15.0% in the TCGA cohort and 17.9% in the GEO cohort) exhibited favorable clinical prognosis, activated metabolic activity, exhausted cell cycle, strong immune infiltration, and lower tumor purity. Four liver-specific matrisome genes (F9, HPX [hemopexin], IGFALS [insulin-like growth-factor-binding protein, acid labile subunit], and PLG [plasminogen]) were identified as involved in HBV-related HCC progression and prognosis. Conclusion: This study identified the expression and function of matrisome genes from HBV-related hepatocarcinogenesis, providing major insight to understand HBV-related HCC and develop potential therapeutic opportunities.
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http://dx.doi.org/10.1002/hep4.1741DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8435279PMC
September 2021

[Effect of early acupuncture on cognitive function in patients with vascular dementia after cerebral infarction].

Zhongguo Zhen Jiu 2021 Sep;41(9):979-83

Department of Encephalopathy, Beichen Hospital of TCM Affiliate to Tianjin University of TCM, Tianjin 300400, China.

Objective: To observe the clinical efficacy of early acupuncture for vascular dementia (VD) after cerebral infarction, and explore its possible mechanism.

Methods: A total of 120 patients with VD after cerebral infarction were randomized into an acupuncture combined with medication group (60 cases, 1 case dropped off) and a western medication group (60 cases, 1 case dropped off). In the western medication group, oxiracetam capsules were given orally, 2 capsules each time, 3 times a day. On the basis of the treatment as the western medication group, acupuncture was applied at Baihui (GV 20), Sishencong (EXHN 1), Zhongwan (CV 12), Wailing (ST 26), Xiawan (CV 10), Qihai (CV 6), Guanyuan (CV 4), etc. in the acupuncture combined with medication group, 30 min each time, once a day, 5 days a week. The treatment was given 8 weeks in both groups. Before and after treatment,the scores of minimental state examination (MMSE), Alzheimer's disease assessment scale cognitive part (ADASCog), clock drawing test (CDT), Barthel index were observed, blood flow velocity of middle cerebral artery (MCA) was detected, and the clinical efficacy was evaluated in the two groups.

Results: The total effective rate was 89.8% (53/59) in the acupuncture combined with medication group, which was superior to 76.3% (45/59) in the western medication group (<0.01). Compared before treatment, the subitem scores and total scores of MMSE, ADASCog score, CDT score and Barthel index score after treatment were improved in the two groups (<0.01, <0.05), and the scores in the acupuncture combined with medication group were superior to those in the western medication group (<0.05, <0.01). After treatment, the blood flow velocity of bilateral MCA was increased in the acupuncture combined with medication group (<0.05), which was faster than the western medication group (<0.05).

Conclusion: Early acupuncture could improve cognitive function and activities of daily living in patients with VD after cerebral infarction, its mechanism may be related to improving the blood flow velocity of MCA, promoting blood circulation, and improving cerebral perfusion.
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http://dx.doi.org/10.13703/j.0255-2930.20210117-k0008DOI Listing
September 2021

Responses of rhizosphere bacterial communities, their functions and their network interactions to Cd stress under phytostabilization by Miscanthus spp.

Environ Pollut 2021 Oct 28;287:117663. Epub 2021 Jun 28.

School of Life Science and Agricultural Engineering, Nanyang Normal University, Nanyang, 473061, China. Electronic address:

Miscanthus has good tolerance to heavy metals (HMs) and has received increasing attention in studies of HM-contaminated soil remediation. In this study, four Miscanthus cultivars (M. lutarioriparius Xiangnadi NO4, M. sinensis Xiangmang NO1, M. lutarioriparius × M. sinensis hybrid Xiangzamang NO1, and M. floridulus Wujiemang NO1) that grow in China were studied. Their tolerance and enrichment abilities in soils containing 50 mg kg cadmium (Cd) and the structure and function of their rhizosphere bacterial communities during the remediation process were analyzed. The results exhibiting a tolerance index (TI) higher than 75 in roots and the aboveground parts (TI > 60, indicating highly tolerant plants) indicated that all four Miscanthus cultivars were tolerant to high Cd concentrations. Moreover, Cd was mainly enriched in roots, the translocation ability from roots to aboveground parts was weak, and the four cultivars exhibited phytostabilization ability in Cd-contaminated soils. High-throughput sequencing (HTS) analysis showed that the Miscanthus rhizosphere bacterial community comprised 33 phyla and 446 genera, including plant growth-promoting rhizobacteria (PGPRs), such as Bacillus, Sphingomonas, and Mesorhizobium. The addition of Cd affected the Miscanthus rhizosphere bacterial community and reduced community diversity. Phylogenetic molecular ecological networks (pMENs) indicated that Cd addition reduced interactions between Miscanthus rhizosphere bacteria and thereby led to a simpler network structure, increased the number of negative-correlation links, enhanced the competition between rhizosphere bacterial species, reduced the number of key bacteria, and changed the composition of those bacteria. PICRUSt functional predictive analysis indicated that Cd stress reduced soil bacterial functions in the Miscanthus rhizosphere. The results of this study provide a basis for the remediation of Cd-contaminated soils by Miscanthus and provide a reference for the subsequent regulation of Miscanthus remediation efficiency by PGPRs or key bacteria.
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http://dx.doi.org/10.1016/j.envpol.2021.117663DOI Listing
October 2021

Free-breathing multitasking multi-echo MRI for whole-liver water-specific T , proton density fat fraction, and quantification.

Magn Reson Med 2021 Aug 21. Epub 2021 Aug 21.

Biomedical Imaging Research Institute, Cedars-Sinai Medical Center, Los Angeles, California, USA.

Purpose: To develop a 3D multitasking multi-echo (MT-ME) technique for the comprehensive characterization of liver tissues with 5-min free-breathing acquisition; whole-liver coverage; a spatial resolution of 1.5 × 1.5 × 6 mm ; and simultaneous quantification of T , water-specific T (T ), proton density fat fraction (PDFF), and .

Methods: Six-echo bipolar spoiled gradient echo readouts following inversion recovery preparation was performed to generate T , water/fat, and contrast. MR multitasking was used to reconstruct the MT-ME images with 3 spatial dimensions: 1 T recovery dimension, 1 multi-echo dimension, and 1 respiratory dimension. A basis function-based approach was developed for T quantification, followed by the estimation of and T -corrected PDFF. The intrasession repeatability and agreement against references of MT-ME measurements were tested on a phantom and 15 clinically healthy subjects. In addition, 4 patients with confirmed liver diseases were recruited, and the agreement between MT-ME measurements and references was assessed.

Results: MT-ME produced high-quality, coregistered T , T , PDFF, and maps with good intrasession repeatability and substantial agreement with references on phantom and human studies. The intra-class coefficients of T , T , PDFF, and from the repeat MT-ME measurements on clinically healthy subjects were 0.989, 0.990, 0.999, and 0.988, respectively. The intra-class coefficients of T , PDFF, and between the MT-ME and reference measurements were 0.924, 0.987, and 0.975 in healthy subjects and 0.980, 0.999, and 0.998 in patients. The T was independent to PDFF (R = -0.029, P = .904).

Conclusion: The proposed MT-ME technique quantifies T , T , PDFF, and simultaneously and is clinically promising for the comprehensive characterization of liver tissue properties.
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http://dx.doi.org/10.1002/mrm.28970DOI Listing
August 2021

Intestinal dysbiosis in nonalcoholic fatty liver disease (NAFLD): focusing on the gut-liver axis.

Crit Rev Food Sci Nutr 2021 Aug 18:1-18. Epub 2021 Aug 18.

State Key Laboratory of Animal Nutrition, Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing, China.

Nonalcoholic fatty liver disease (NAFLD) is one of the most common chronic liver disorders in humans, partly because it is closely related to metabolic disorders of the liver with increasing prevalence. NAFLD begins with hepatic lipid accumulation, which may cause inflammation and eventually lead to fibrosis in the liver. Numerous studies have demonstrated the close relationship between gut dysfunction (especially the gut microbiota and its metabolites) and the occurrence and progression of NAFLD. The bidirectional communication between the gut and liver, named the gut-liver axis, is mainly mediated by the metabolites derived from both the liver and gut through the biliary tract, portal vein, and systemic circulation. Herein, we review the effects of the gut-liver axis on the pathogenesis of NAFLD. We also comprehensively describe the potential molecular mechanisms from the perspective of the role of liver-derived metabolites and gut-related components in hepatic metabolism and inflammation and gut health, respectively. The study provides insights into the mechanisms underlying current summarizations that support the intricate interactions between a disordered gut and NAFLD and can provide novel strategies to lessen the prevalence and consequence of NAFLD.
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http://dx.doi.org/10.1080/10408398.2021.1966738DOI Listing
August 2021

Oseltamivir Improved Thrombocytopenia During Veno-Arterial Extracorporeal Membrane Oxygenation in Adults With Refractory Cardiac Failure: A Single-Center Retrospective Real-World Study.

Front Cardiovasc Med 2021 26;8:645867. Epub 2021 Jul 26.

Qilu Hospital, Shandong University, Jinan, China.

Severe thrombocytopenia is a common complication of extracorporeal membrane oxygenation (ECMO). Oseltamivir can be used to treat infection-associated thrombocytopenia. To evaluate the effect of oseltamivir on attenuating severe thrombocytopenia during ECMO. This was a single-center real-world study in critically ill patients supported with venous-arterial extracorporeal membrane oxygenation (VA-ECMO). Patients suspected or confirmed with influenza received oseltamivir according to the Chinese guidelines. Thrombocytopenia and survival were compared between the oseltamivir-treated and untreated group. The factors associated with survival were analyzed by multivariable Cox analysis. A total of 82 patients were included. All patients developed thrombocytopenia after initiating VA-ECMO. Twenty-three patients received oseltamivir (O group), and 59 did not use oseltamivir (O group). During the first 8 days after VA-ECMO initiation, the platelet count in the O group was higher than that in the O group (all < 0.05). The patients in the O group had a higher median nadir platelet count (77,000/μl, 6,000-169,000/μl) compared with the O group (49,000/μl, 2,000-168,000/μl; = 0.04). A nadir platelet count of <50,000/μl was seen in 26% of the patients in the O group, compared with 53% in the O group ( = 0.031). No significant difference in survival from cardiac failure was seen between the O and O group (48 vs. 56%, = 0.508). The Sequential Organ Failure Assessment (SOFA) score on initiation of VA-ECMO were independently associated with survival (OR = 1.12, 95% confidence interval (95% CI): 1.02-1.22, = 0.015). Oseltamivir could ameliorate VA-ECMO-related thrombocytopenia. These findings suggested the prophylactic potential of oseltamivir on severe thrombocytopenia associated with the initiation of VA-ECMO.
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http://dx.doi.org/10.3389/fcvm.2021.645867DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8349981PMC
July 2021

METTL1/WDR4-mediated mG tRNA modifications and mG codon usage promote mRNA translation and lung cancer progression.

Mol Ther 2021 Aug 8. Epub 2021 Aug 8.

Center for Translational Medicine, Institute of Precision Medicine, The First Affiliated Hospital, Sun Yat-sen University, Guangzhou 510080, China; State Key Laboratory of Oncology in South China, Sun Yat-sen University Cancer Center, Guangzhou 510060, China. Electronic address:

Mis-regulated epigenetic modifications in RNAs are associated with human cancers. The transfer RNAs (tRNAs) are the most heavily modified RNA species in cells; however, little is known about the functions of tRNA modifications in cancers. In this study, we uncovered that the expression levels of tRNA N-methylguanosine (mG) methyltransferase complex components methyltransferase-like 1 (METTL1) and WD repeat domain 4 (WDR4) are significantly elevated in human lung cancer samples and negatively associated with patient prognosis. Impaired mG tRNA modification upon METTL1/WDR4 depletion resulted in decreased cell proliferation, colony formation, cell invasion, and impaired tumorigenic capacities of lung cancer cells in vitro and in vivo. Moreover, gain-of-function and mutagenesis experiments revealed that METTL1 promoted lung cancer growth and invasion through regulation of mG tRNA modifications. Profiling of tRNA methylation and mRNA translation revealed that highly translated mRNAs have higher frequencies of mG tRNA-decoded codons, and knockdown of METTL1 resulted in decreased translation of mRNAs with higher frequencies of mG tRNA codons, suggesting that tRNA modifications and codon usage play an essential function in mRNA translation regulation. Our data uncovered novel insights on mRNA translation regulation through tRNA modifications and the corresponding mRNA codon compositions in lung cancer, providing a new molecular basis underlying lung cancer progression.
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http://dx.doi.org/10.1016/j.ymthe.2021.08.005DOI Listing
August 2021

The lncRNA H19/miR-541-3p/Wnt/β-catenin axis plays a vital role in melatonin-mediated osteogenic differentiation of bone marrow mesenchymal stem cells.

Aging (Albany NY) 2021 07 26;13(14):18257-18273. Epub 2021 Jul 26.

Department of Oral and Maxillofacial Surgery, Jinan Stomatology Hospital, Jinan 250001, Shandong Province, China.

Implant dentures become the first choice for denture restoration in patients with tooth loss. However, oral implants often fail in osteoporosis (OP) patients. Melatonin (MT) induces osteogenic differentiation of bone mesenchymal stem cells (BMSCs), suggesting its therapeutic potential in OP treatment. Long non-coding RNA H19 induces osteogenic differentiation of BMSCs, while its regulatory mechanism in MT-involved osteogenic and adipogenic differentiation of BMSCs remains elusive. Ovariectomized (OVX) rat was used to construct an OP model, and bone quality was assessed. Meanwhile, the expression of H19, miR-541-3p, MT and adiponectin (APN) was examined by quantitative reverse transcription-PCR (qRT-PCR) or ELISA. The adipogenic and osteogenic differentiation of BMSCs were determined by oil red O staining and alizarin red S staining, respectively. The targeting relationships between H19, miR-541-3p and APN mRNA were predicted by bioinformatics and confirmed by RNA immunoprecipitation and dual-luciferase reporter assay. The results showed that MT, H19 and APN were down-regulated, while miR-541-3p was up-regulated in the OVX rat model. At the cellular level, MT reduced adipogenic differentiation, heightened osteogenic differentiation of BMSCs, and activated Wnt/β-catenin pathway, which were reversed by the MT2 selective inhibitor 4-P-PDOT. Overexpressing H19 facilitated the osteogenic differentiation and inhibited the adipogenic differentiation of BMSCs mediated by MT, while H19 knockdown or overexpressing miR-541-3p had the opposite effect. Moreover, H19 functioned as a competitive endogenous RNA and sponged miR-541-3p, and miR-541-3p targeted APN. Overall, MT modulates the osteogenic and adipogenic differentiation of BMSCs by mediating H19/miR-541-3p/APN axis, providing a new reference for the targeted therapy of OP.
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http://dx.doi.org/10.18632/aging.203267DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8351702PMC
July 2021

[Shifts in Rhizosphere Bacterial Community Structure, Co-occurrence Network, and Function of Following Cadmium Exposure].

Huan Jing Ke Xue 2021 Aug;42(8):3997-4004

School of Life Science and Agricultural Engineering, Nanyang Normal University, Nanyang 473061, China.

As a representative of second-generation bioenergy plants, has received increasing attention in the studies of heavy metal (HM)-contaminated soil remediation. Currently, few studies have examined the effects of using to remediate HM-contaminated soils on the composition and function of microbial communities. In this study, the cultivar was examined for its tolerance and enrichment abilities when grown in soils containing 100 mg ·kg of cadmium (Cd). The structure, function, and co-occurrence network of their rhizosphere bacterial communities were analyzed during the remediation process. MiSeq sequencing showed that the rhizosphere bacterial community comprised 32 phyla and 425 genera, including plant growth-promoting rhizobacteria (PGPR), such as , , , and . The addition of Cd affected the rhizosphere bacterial community and reduced community diversity. Phylogenetic molecular ecological networks indicated that Cd addition reduced the interactions between rhizosphere bacteria to generate a simpler network structure, increased the number of negative-correlation links, enhanced the competition between rhizosphere bacterial species, and changed the composition of key bacteria. PICRUSt functional predictive analysis indicated that Cd stress reduced soil bacterial functions in the rhizosphere. The results of this study provide a reference for the subsequent regulation of efficient remediation by PGPRs or key bacteria.
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http://dx.doi.org/10.13227/j.hjkx.202011198DOI Listing
August 2021

Dual flip-angle IR-FLASH with spin history mapping for B1+ corrected T1 mapping: Application to T1 cardiovascular magnetic resonance multitasking.

Magn Reson Med 2021 Jul 26. Epub 2021 Jul 26.

Biomedical Imaging Research Institute, Cedars-Sinai Medical Center, Los Angeles, California, USA.

Purpose: To develop a single-scan method for -corrected T mapping and apply it for free-breathing (FB) cardiac MR multitasking without electrocardiogram (ECG) triggering.

Methods: One dual flip-angle (2FA) inversion recovery (IR)-FLASH scan provides two observations of (apparent T ) corresponding to two distinct combinations of the nominal FA α and . Spatiotemporally coregistered T and spin history maps are obtained by fitting the 2FA signal model. T estimate accuracy and repeatability for single flip-angle (1FA) and 2FA IR-FLASH sequence MR multitasking were evaluated at 3T. A T phantom was first imaged on the scanner table, then on two human subjects' thoraxes in both breath-hold (BH) and FB conditions. IR-turbo spin echo (IR-TSE) static phantom T measurements served as reference. In 10 healthy subjects, myocardial T was evaluated with ECG-free, FB multitasking sequences alongside ECG-triggered BH MOLLI.

Results: For phantom-on-table T estimates, 2FA agreed better with IR-TSE (intraclass correlation coefficient [ICC] = 0.996, mean error ± SD = -1.6% ± 1.9%) than did 1FA (ICC = 0.922; mean error ± SD = -4.3% ± 12%). For phantom-on-thorax, 2FA was more repeatable and robust to respiration than 1FA (coefficient of variation [CoV] = 1.2% 2FA, = 11.3% 1FA). In vivo, in intrasession T repeatability, 2FA (septal CoV = 2.4%, six-segment CoV = 4.4%) outperformed 1FA (septal CoV = 3.1%, six-segment CoV = 5.5%). In six-segment T homogeneity, 2FA (CoV = 7.9%) also outperformed 1FA (CoV = 11.1%).

Conclusion: The 2FA IR-FLASH improves T estimate accuracy and repeatability over 1FA IR-FLASH, and enables single-scan -corrected T mapping without BHs or ECG when used with MR multitasking.
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http://dx.doi.org/10.1002/mrm.28935DOI Listing
July 2021

From gut microbiota to host appetite: gut microbiota-derived metabolites as key regulators.

Microbiome 2021 07 20;9(1):162. Epub 2021 Jul 20.

State Key Laboratory of Animal Nutrition, Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing, 100193, China.

Feelings of hunger and satiety are the key determinants for maintaining the life of humans and animals. Disturbed appetite control may disrupt the metabolic health of the host and cause various metabolic disorders. A variety of factors have been implicated in appetite control, including gut microbiota, which develop the intricate interactions to manipulate the metabolic requirements and hedonic feelings. Gut microbial metabolites and components act as appetite-related signaling molecules to regulate appetite-related hormone secretion and the immune system, or act directly on hypothalamic neurons. Herein, we summarize the effects of gut microbiota on host appetite and consider the potential molecular mechanisms. Furthermore, we propose that the manipulation of gut microbiota represents a clinical therapeutic potential for lessening the development and consequence of appetite-related disorders. Video abstract.
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http://dx.doi.org/10.1186/s40168-021-01093-yDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8293578PMC
July 2021

Assessment of intramyocardial hemorrhage with dark-blood T2*-weighted cardiovascular magnetic resonance.

J Cardiovasc Magn Reson 2021 07 15;23(1):88. Epub 2021 Jul 15.

Department of Biomedical Sciences, Cedars-Sinai Medical Center, Biomedical Imaging Research Institute, PACT Bldg - Suite 400, 8700 Beverly Blvd, Los Angeles, CA, USA.

Background: Intramyocardial hemorrhage (IMH) within myocardial infarction (MI) is associated with major adverse cardiovascular events. Bright-blood T2*-based cardiovascular magnetic resonance (CMR) has emerged as the reference standard for non-invasive IMH detection. Despite this, the dark-blood T2*-based CMR is becoming interchangeably used with bright-blood T2*-weighted CMR in both clinical and preclinical settings for IMH detection. To date however, the relative merits of dark-blood T2*-weighted with respect to bright-blood T2*-weighted CMR for IMH characterization has not been studied. We investigated the diagnostic capacity of dark-blood T2*-weighted CMR against bright-blood T2*-weighted CMR for IMH characterization in clinical and preclinical settings.

Materials And Methods: Hemorrhagic MI patients (n = 20) and canines (n = 11) were imaged in the acute and chronic phases at 1.5 and 3 T with dark- and bright-blood T2*-weighted CMR. Imaging characteristics (Relative signal-to-noise (SNR), Relative contrast-to-noise (CNR), IMH Extent) and diagnostic performance (sensitivity, specificity, accuracy, area-under-the-curve, and inter-observer variability) of dark-blood T2*-weighted CMR for IMH characterization were assessed relative to bright-blood T2*-weighted CMR.

Results: At both clinical and preclinical settings, compared to bright-blood T2*-weighted CMR, dark-blood T2*-weighted images had significantly lower SNR, CNR and reduced IMH extent (all p < 0.05). Dark-blood T2*-weighted CMR also demonstrated weaker sensitivity, specificity, accuracy, and inter-observer variability compared to bright-blood T2*-weighted CMR (all p < 0.05). These observations were consistent across infarct age and imaging field strengths.

Conclusion: While IMH can be visible on dark-blood T2*-weighted CMR, the overall conspicuity of IMH is significantly reduced compared to that observed in bright-blood T2*-weighted images, across infarct age in clinical and preclinical settings at 1.5 and 3 T. Hence, bright-blood T2*-weighted CMR would be preferable for clinical use since dark-blood T2*-weighted CMR carries the potential to misclassify hemorrhagic MIs as non-hemorrhagic MIs.
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http://dx.doi.org/10.1186/s12968-021-00787-4DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8281666PMC
July 2021

Development and Validation of a Nomogram for the Prediction of Inguinal Lymph Node Metastasis Extranodal Extension in Penile Cancer.

Front Oncol 2021 17;11:675565. Epub 2021 Jun 17.

Department of Urology, Sun Yat-sen University Cancer Center, Guangzhou, China.

Purpose: To determine whether a clinicopathologic and laboratory-based nomogram is capable of predicting the risk of lymph node extranodal extension (ENE) in patients with penile cancer.

Materials And Methods: From June 2006 to January 2021, 234 patients who underwent bilateral inguinal lymph node dissection (ILND) surgery were included in the analysis. A Lasso regression model was utilized to select the most useful predictive features from among 46 laboratory variables. Then, a logistic regression analysis was used to develop the prediction model. Calibration curves, concordance index (C-index) and Areas under the receiver-operating characteristic curves (AUCs) were performed to evaluate the performance of the nomogram. We also investigated model fit using changes in Akaike Information Criteria (AICs). Decision curve analyses (DCAs) were applied to assess the clinical usefulness of this nomograms. Its internal validation was confirmed.

Results: Among the 234 patients, 53 were confirmed to have ENE. The platelet-lymphocyte ratio (PLR) and Squamous cell carcinoma antigen (SCC-Ag) were significantly associated with ENE (P<0.05). The individualized prediction nomogram, including the PLR, SCC-Ag, lymphovascular invasion (LVI), and pathologic tumor stage(pT-stage), showed good discrimination, with a C-index of 0.817 (95% CI, 0.745 to 0.890) and good calibration. Clinical-laboratory nomogram (AIC, 180.034) become the best-fitting model. DCA findings revealed that the clinical-laboratory nomogram was more clinically useful than the pT-stage or tumor grade.

Conclusions: This study presents a clinicopathologic and laboratory-based nomogram that incorporates PLR, SCC-Ag, lymphovascular invasion (LVI), and pT-stage, which can be conveniently utilized to facilitate the individualized prediction of lymph node metastasis ENE in patients with penile cancer.
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http://dx.doi.org/10.3389/fonc.2021.675565DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8247463PMC
June 2021

Pelvic Lymph Node Dissection in Penile Cancer With Inguinal Lymph Node Extranodal Extension: A Multicenter Experience.

Front Surg 2021 15;8:644273. Epub 2021 Jun 15.

Department of Urology, Sun Yat-sen University Cancer Center, Guangzhou, China.

The aim of this study is to determine the necessary extent of penile lymph node dissection (PLND) in penile cancer patients with inguinal lymph node extracapsular extension (ILN-ENE). Penile cancer patients who underwent PLND in 15 centers from January 2006 to April 2020 were retrospectively analyzed. PLND was performed in patients with ILN-ENE. Sixty-two patients with ILN-ENE were included in the analysis. A total of 51.6% (32/62) of the patients were confirmed to have pelvic lymph node metastasis (PLNM), and 31.3% (10/32) of patients were confirmed to have multiple PLNMs. Of the patients with metastases, 59.4% (19/32) had bilateral inguinal lymph node metastasis (ILNM). According to the anatomical structure, 71.9% (23/32) of the patients had PLNM in the external iliac region, and 56.2% (18/32) had PLNM in the obturator region. Among those with oligo-PLNM, 65.1% (28/43) of the patients had PLNM in the external iliac region and 38.9% (15/43) had PLNM in the obturator region. A significant overall survival difference was observed between patients with the bilateral ILNM and unilateral ILNM (36-month: 21.2 vs. 53.7%, respectively = ). Patients with bilateral ILNM had relatively poor metastasis-free survival compared with unilateral ILNM (36-month: 33.0 vs. 13.9%, respectively = ). The external iliac and obturator region were the most commonly affected regions in patients with ILN-ENE, and these regions were the only affected regions in patients with oligo-PLNM. Patients with bilateral ILNM had a high risk of PLNM and worse survival.
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http://dx.doi.org/10.3389/fsurg.2021.644273DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8239133PMC
June 2021

Dynamics of Diversity and Abundance of Sulfonamide Resistant Bacteria in a Silt Loam Soil Fertilized by Compost.

Antibiotics (Basel) 2021 Jun 11;10(6). Epub 2021 Jun 11.

College of Resources and Environmental Science, Beijing Key Laboratory of Biodiversity and Organic Farming, China Agricultural University, Yuanmingyuan West Road No.2, Haidian District, Beijing 100193, China.

Although composting is effective in deactivating antibiotic substances in manure, the influence of compost fertilization on the occurrence and dissemination of antibiotic resistance in arable soils remains to be controversial. Herein, the abundance and diversity of two sulfonamide resistance genes ( and ) in soil fertilized by compost spiked with two concentrations of sulfadiazine (1 and 10 mg kg) were studied intensively by qPCR and high throughput sequencing based on a two-month microcosm experiment. The concentration of sulfadiazine decreased rapidly after spiking from 25% at Day 1 to less than 2.7% at Day 60. Relative abundance of both and were significantly higher in soil amended with compost than the non-amended control at Day 1 and slightly decreased with incubation time except for in the S10 treatment. Soil bacterial communities were transiently shifted by compost fertilization regardless of the presence of sulfadiazine. Relative abundance of genera in three hubs positively interlinked with and were significantly higher in compost treated soil than the control at Day 1, 7 and 21, but not at Day 60. High throughput sequencing analyses revealed that most detected (>67% in relative abundance) and genotypes sharing >99% similarity with those found in gammaproteobacterial pathogens frequently were commonly present in compost and soil. These results indicated that compost fertilization might increase the abundance rather than diversity of sulfadiazine-resistant populations in soil, which may be facilitated by the presence of sulfadiazine.
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http://dx.doi.org/10.3390/antibiotics10060699DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8230599PMC
June 2021

Role of polystyrene microplastics in sunlight-mediated transformation of silver in aquatic environments: Mechanisms, kinetics and toxicity.

J Hazard Mater 2021 10 21;419:126429. Epub 2021 Jun 21.

Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan 430072, China; China University of Chinese Academy of Sciences, Beijing 100049, China. Electronic address:

Sunlight-oxidative ageing is a common and critical process for microplastics (MPs) in aquatic environments. O, O, and •OH generation has been widely proven in this process, which can alter metal speciation based on its reduction and oxidation potential. Herein, chemical speciation of Ag mediated by polystyrene (PS) MPs was determined under simulated sunlight irradiation. The O generation on the PS MPs surfaces is the vital factor for Ag reduction, regardless of acid or base conditions. The O and •OH are dominant factors, and O played a more important role than •OH for its higher formation amount, causing oxidative dissolution of newly formed Ag nanoparticles (NPs). The Ag NPs can hetero-aggregate with PS MPs through electrostatic interactions with O-containing groups (C-O, C-OH and CO), and co-precipitate from the water phase. This hetero-aggregation can stabilize Ag NPs by inhibiting Ag NPs surface photooxidation and suppressing Ag release. Transformation of Ag species (from Ag to Ag NPs) mediated by sunlight with PS MPs significantly suppressed acute toxicity of Ag to Escherichia coli, Selenastrum capricornutum, Daphnia magna and zebrafish. This study emphasized that PS MPs play an important role in the speciation, migration and toxicity of Ag in freshwater environments.
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http://dx.doi.org/10.1016/j.jhazmat.2021.126429DOI Listing
October 2021
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