Publications by authors named "Hui Ding"

740 Publications

Studies on Chemical Characterization of Ginkgo Amillaria Oral Solution and Its Drug-Drug Interaction With Piceatannol 3'---D-Glucopyranoside for Injection.

Front Pharmacol 2022 19;13:932646. Epub 2022 Jul 19.

State Key Laboratory of Component-Based Chinese Medicine, Tianjin University of Traditional Chinese Medicine, Tianjin, China.

Ginkgo Amillaria oral solution (GAO) is commonly used for the treatment of cardiovascular and cerebrovascular diseases in China. Piceatannol-3'---D-glucopyranoside for injection (PGI) is mainly used for the prevention and treatment of ischemic cerebrovascular diseases. With the spread of cerebrovascular disease, the possibility of combining the two drugs has increased; however, there is no research on the drug-drug interaction (DDI) between these two medicines. In this paper, an ultrahigh-performance liquid chromatography/quadrupole-orbitrap mass spectrometry (UHPLC/Q-Orbitrap MS) method was established to characterize the chemical constituents of GAO first; 62 compounds were identified or tentatively identified based on their retention time (RT), MS, and MS/MS data. Nine main compounds were determined by ultrahigh-performance liquid chromatography/triple quadrupole mass spectrometry (UPLC-QQQ-MS). Furthermore, incubation with liver microsomes was fulfilled; the results showed that GAO had a significant inhibitory effect on UGT1A9 and UGT2B7 ( < 0.05), and PGI was mainly metabolized by UGT1A9. The identification results of metabolites of PGI showed that PGI mainly undergoes a phase II binding reaction mediated by UDP-glucuronosyltransferase (UGT) and sulfotransferase (SULT) . Therefore, pharmacokinetic studies were performed to investigate the DDI between GAO and PGI. The results showed that the AUC ( < 0.05) and T ( < 0.05) of PGI were significantly increased when administered together with GAO, whereas the CL was significantly decreased ( < 0.05). The exploration of and experiments showed that there was a DDI between GAO and PGI.
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http://dx.doi.org/10.3389/fphar.2022.932646DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9344054PMC
July 2022

Psychological coherence, inclusive leadership and implicit absenteeism in obstetrics and gynecology nurses: a multi-site survey.

BMC Psychiatry 2022 08 3;22(1):525. Epub 2022 Aug 3.

Department of Education, The First Affiliated Hospital of USTC, Division of Life Sciences and Medicine, University of Science and Technology of China, Hefei, Anhui, 230001, China.

Background: Implicit absenteeism is very common among clinical nurses. We aimed to evaluate the role of psychological coherence in the inclusive leadership and implicit absenteeism among obstetrics and gynecology nurses, to provide evidence to the clinical management of nurses.

Methods: Through the convenience sampling method, a survey of gynecology nurses in tertiary hospitals in 16 cities of Anhui Province, China was conducted using the General Information Questionnaire, the Stanford Implicit Absence Scale, the Inclusive Leadership Scale and the Sense of Coherence Scale. Statistical analysis was performed by SPSS 20.

Results: A total of 1080 nurses were included with an effective response rate of 93.5%. The average score of nurses' recessive absenteeism in this study was (16.8 ± 0.15). The average of inclusive leadership score was (34.25 ± 7.23). The average score of psychological coherence score of obstetrics and gynecology nurses was (55.79 ± 8.28). Pearson correlation analysis showed that there was a relationship between implicit absenteeism behavior, inclusive leadership, and the level of psychological coherence in obstetrics and gynecology nurses (all P < 0.05). Linear regression analysis indicated that psychological coherence played a partial mediating role between inclusive leadership and obstetrics and gynecology nurses' implicit absenteeism (all P < 0.05).

Conclusions: Obstetrics and gynecology nurses have serious recessive absenteeism with low sense of psychological coherence and inclusive leadership. Nursing managers should improve the psychological coherence through effective interventions, thereby reducing the incidence of implicit absenteeism.
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http://dx.doi.org/10.1186/s12888-022-04137-1DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9351111PMC
August 2022

Ultra high-performance liquid chromatography coupled to ion mobility quadrupole time of flight mass spectrometry profiling and unveiling the transformation of ginsenosides by the dual conditions of citric acid and high-pressure steaming.

Rapid Commun Mass Spectrom 2022 Jul 28:e9363. Epub 2022 Jul 28.

College of Pharmaceutical Engineering of Traditional Chinese Medicine, Tianjin University of Traditional Chinese Medicine No.10 Poyanghu Road, West Tuanbo New Town, Jinghai District, Tianjin, P. R. China.

Rationale: Many methods have been reported to produce rare ginsenosides, including heat treatment, acid hydrolysis, alkaline hydrolysis, enzymatic hydrolysis, and microbial transformation. However, the conversion of original ginsenosides to rare ginsenosides in the dual conditions of citric acid and high-pressure steam sterilization pot was rarely reported.

Methods: In this study, an UHPLC/IM-QTOF-MS method was developed for analysis of chemical transformation of PPT-type ginsenosides Rg , Re, PPD-type ginsenoside Rb and total ginsenosides in the dual conditions of citric acid and high-pressure steam sterilization pot. An internal ginsenoside database containing 126 known ginsenosides and 18 ginsenoside reference compounds was established to identify the transformation products and explore possible transformation pathways and mechanisms.

Results: A total of 54 ginsenosides have been preliminarily identified in the transformation products of PPD-type ginsenosides Rg , Re, PPD-type ginsenoside Rb and total ginsenosides, and the possible transformation pathways were as follows: Rg , Re→20(S)-Rh , 20(R)-Rh ; Rg , Re→20(S)-Rh , 20(R)-Rh →Rk , Rh , Rh ; Rb →gypenoside LXXV; Rb →20(S)-Rg , 20(R)-Rg →Rk , Rg ; Re→20(S)-Rg , 20(R)-Rg →20(S)-Rf , 20(R)-Rf , Rg , F .

Conclusions: The results elucidated the possible transformation pathways and mechanisms of ginsenosides in the dual conditions of citric acid and high-pressure steam sterilization pot, which was helpful to reveal the mechanisms of ginsenosides and enhanced safety and quality control of pharmaceuticals and nutraceuticals. Meanwhile, as a simple, efficient and practical method was developed for the production of rare ginsenosides, which has the potential to produce diverse rare ginsenosides on an industrial scale.
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http://dx.doi.org/10.1002/rcm.9363DOI Listing
July 2022

PD-L1 and the Clinical Outcomes of Ovarian Cancer: Meta-Analysis and Bioinformatical Analysis.

Asian Pac J Cancer Prev 2022 07 1;23(7):2285-2290. Epub 2022 Jul 1.

Department of Toxicology, Guilin Medical University, Guilin 541004, China.

Objective: A meta-analysis was performed to analyze the association between PD-L1 expression and overall survival (OS) in various tumors and to identify potential targets through biological information analysis.

Methods: the data were collected from PubMed and Cochrane library, the all analysis of our study were conducted by STATA software and online website.

Results: Ten articles (including 11 studies) that met all inclusion criteria were obtained. The combined HR showed that high PD-L1 expression was significantly associated with poor overall survival (HR = 1.84, 95% CI: 1.15-2.93). Pathway analysis revealed that the upregulated genes were primarily involed in biological processes, including nucleic acid transcription, biosynthesis and negative regulation of cell metabolism. The downregulated genes were primarily involed in the regulation of cell cycle, including chromosome separation and DNA metabolism. The top ten genes that were identified were hub genes (CDK1, CCNB1, CCNA2, KIF11, CDC20, UBE2C, NCAPG, AURKA, AURKB, CHEK1), which had significant function in cell differentiation and virus infection. The Kaplan-Meier survival curve indicated that CCNB1, KIF11, UBE2C, NCAPG, AURKA and CHEK1 were statistically significant (P<0.05).

Conclusion: PD-L1 was found to be a latent biomarker for predicting the prognostic value of cancer and also a therapeutic target.
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http://dx.doi.org/10.31557/APJCP.2022.23.7.2285DOI Listing
July 2022

Prognostic value of preoperative platelet-related parameters and plasma fibrinogen in patients with non-muscle invasive bladder cancer after transurethral resection of bladder tumor.

Future Oncol 2022 Aug 26;18(26):2933-2942. Epub 2022 Jul 26.

Institute of Urology, Lanzhou University Second Hospital, Key Laboratory of Gansu Province for Urological Diseases, Clinical Center of Gansu Province for Nephro-urology, Lanzhou University, Lanzhou, China.

To investigate the prognostic value of preoperative mean platelet volume (MPV), MPV/lymphocyte ratio (MPVLR), MPV/platelet count ratio and plasma fibrinogen in patients with non-muscle invasive bladder cancer (NMIBC) after transurethral resection of bladder tumor (TURBT). A total of 371 patients who underwent TURBT were enrolled. The main end points were disease-free survival (DFS) and overall survival (OS). MPVLR, tumor size, tumor number and pathological grade were independent risk factors for postoperative DFS. Age and pathological grade were independent risk factors for postoperative OS. MPVLR is an independent risk factor for DFS in NMIBC patients and could be used as a parameter to predict postoperative tumor recurrence in patients after TURBT.
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http://dx.doi.org/10.2217/fon-2022-0223DOI Listing
August 2022

[Effects of pre-moxibustion on expression of phosphorylated Tau protein and related protein kinases in hippocampal CA3 region of AD rats].

Zhen Ci Yan Jiu 2022 Jul;47(7):573-9

College of Acupuncture and Massage, Anhui University of Chinese Medicine, Hefei 230031.

Objective: To observe the effect of pre-moxibustion at "Baihui"(GV20), "Shenshu"(BL23) and "Zusanli"(ST36) on expression of Tau protein and related protein kinases as glycogen synthase kinase-3β (GSK-3β), etc. in the hippocampal CA3 region of Alzheimer's disease (AD) rats, so as to explore its mechanism underlying prevention and treatment of AD cognitive impairment.

Methods: Male SD rats were randomly divided into 4 groups: normal control, sham operation, model and pre-moxibustion,with 9 rats in each group. Rats of the pre-moxibustion group received moxibustion of GV20, BL23 and ST36 for 15 min, once a day, 6 days a week for 3 weeks. After completion of moxibustion, the AD model was reproduced by injection of amyloid beta-peptide 25-35(Aβ 25-35) aggregation solution 1 μL (5 μg/μL) into the bilateral hippocampus, rats of the sham operation group received injection of the same dose of normal saline into the hippocampus. The spatial learning-memory ability was detected using Morris water maze test, and changes of the ultrastructure of hippocampal neurons were observed using electron microscope, and those of histopathological changes of hippocampus tissue observed using hematoxylin eosin (H.E.) staining. The expression levels of hippocampal GSK-3β, p-Tau, CDK5 and Synapsin I proteins were detected by Western blot and immunohistochemistry, respectively.

Results: No significances were found between the normal control and sham groups in all the indexes (>0.05). Compared with the control group, the escape latency of place navigation test of Morris water maze test, expression of GSK-3β and CDK5 and the immunoactivity of GSK-3β, CDK5 and p-Tau were significantly increased (<0.01), and the residence time in the platform quadrant and the number of platform crossing of spatial prob test and the expression of Synapsin Ⅰ significantly reduced in the model group (<0.01). Following the intervention, the increase of escape latency, expression of GSK-3β and CDK5 and the immunoactivity of GSK-3β, CDK5 and p-Tau, and the decrease of residence time in the platform quadrant, number of platform crossing and the expression of Synapsin Ⅰ were reversed in the pre-moxibustion group (<0.05, <0.01). Outcomes of ultrastructure and histopathological observations respectively showed edema of hippocampal nerve cells at varying degrees, moderate edema of the cytoplasma, chromatin condensation at the edge of the nucleus, partial mitochondrial vacuole-like degeneration, fracture of tubular crest, edema and expansion of Golgi body, disappearance of polarity, fracture of the rough endoplasmic reticulum, degeneration of ribosome and partial myelin axon and reduced synaptic vesicles in the presynaptic capsule; and reduced number of neurons with shrank body, disappearance of nucleolus and blurred nuclear boundary and vacuole-like degeneration in some of them in the model group, which were relatively milder in the pre-moxibustion group.

Conclusion: Pre-moxibustion at GV20, BL23 and ST36 plays a role in slowing down the occurrence and development of cognitive impairment in AD rats, which may be related to its functions in inhibiting tau protein hyperphosphorylation and reducing the expression of some related protein kinases in the hippocampus.
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http://dx.doi.org/10.13702/j.1000-0607.20211346DOI Listing
July 2022

Prediction of Retail Price of Sporting Goods Based on LSTM Network.

Authors:
Hui Ding

Comput Intell Neurosci 2022 9;2022:4298235. Epub 2022 Jul 9.

School of Physical Education, Henan University of Science and Technology, Luoyang, Henan 471000, China.

Commodity prices play a unique role as a lever to regulate the economy. Price forecasting is an important part of macrodecision-making and micromanagement. Because there are many factors affecting the price of goods, price prediction has become a difficulty in research. According to the characteristics that price data are also affected by other factors except for time series, a multifactor LSTM price prediction method is proposed based on the long-term and short-term memory network (LSTM) deep learning algorithm. This method not only makes use of the memory of LSTM to historical data but also introduces the influence of external factors on price through the full connection layer, which provides a new idea for solving the problem of price prediction. Compared with BP neural network, the experimental results show that this method has higher accuracy and better stability. Analyze the commodity description and commodity price characteristics, find out the commodities similar to the target commodity, complete the commodity price data by using the historical price data of similar commodities, and establish the training set to verify the validity of the proposed method.
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http://dx.doi.org/10.1155/2022/4298235DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9288340PMC
July 2022

Effect of Thymoquinone on Renal Damage Induced by Hyperlipidemia in LDL Receptor-Deficient (LDL-R/) Mice.

Biomed Res Int 2022 6;2022:7709926. Epub 2022 Jul 6.

Department of Cardiology, Xi'an No. 3 Hospital, The Affiliated Hospital of Northwest University, Xi'an, Shaanxi 710018, China.

Hyperlipidemia is a well-established risk factor for kidney injury, which can lead to chronic kidney disease (CKD). Thymoquinone (TQ) is one of the most active ingredients in Nigella sativa seeds. It has various beneficial properties, including antioxidant and anti-inflammatory activities. TQ also exerts positive effects on doxorubicin- (DOX-) induced nephropathy and ischemia-reperfusion-induced kidney injury in rats. Therefore, in this study, we investigated the possible protective effects of TQ against kidney injury in low-density lipoprotein receptor-deficient (LDL-R/) mice. Eight-week-old male LDL-R/ mice were randomly divided into the following three groups: normal diet (ND group), high-fat diet (HFD group), and HFD combined with TQ (HFD+TQ group). The mice were fed the same diet for eight weeks. After eight weeks, we performed serological analysis of the mice in all three groups. We histologically analyzed the kidney tissue and also investigated the expression of proinflammatory cytokines in the kidney tissue. Metabolic characteristics, including total cholesterol (TC), low-density lipoprotein-cholesterol (LDL-C), and creatinine (CRE) levels, were lower in the LDL-R/ HFD+TQ mice than in the HFD mice. Periodic acid-Schiff (PAS) and Masson's trichrome staining revealed excessive lipid deposition and collagen accumulation in the kidneys of the LDL-R/ HFD mice, which were significantly reduced in the LDL-R/ HFD+TQ mice. Furthermore, macrophages and levels of proinflammatory cytokines were lower in the kidney tissues of the LDL-R/ HFD+TQ mice than in those of the LDL-R/ HFD mice. Moreover, profibrosis- and oxidative stress-related protein expression was lower in the kidney tissues of the LDL-R/ HFD+TQ mice than in those of the LDL-R/ HFD mice. These results indicate that TQ may be a potential therapeutic agent for kidney damage caused by hyperlipidemia.
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http://dx.doi.org/10.1155/2022/7709926DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9279052PMC
July 2022

Is Anti-Reflux Anastomosis an Advantage in an Orthotopic Ileal Neobladder? A Systematic Review and Meta-Analysis.

Urol Int 2022 Jul 14:1-14. Epub 2022 Jul 14.

Department of Urology, Lanzhou University Second Hospital, Institute of Urology, Institute of Gansu Nephro-Urological Clinical Center, Key Laboratory of Urological Disease of Gansu Province, Lanzhou, China.

Introduction: We conducted a systematic review and meta-analysis to assess the available literature regarding the postoperative effects of anti-reflux anastomosis and direct anastomosis in orthotopic ileal neobladder (ONB).

Methods: We searched PubMed, Embase, and the Cochrane Library in October 2021. We included 11 studies of patients with bladder cancer who underwent radical cystectomy and ONB as urinary diversion. Outcomes evaluated in this review were ureteroenteric anastomotic stricture (UEAS), vesicoureteral reflux, renal function (RFn) impairment, and pyelonephritis. All data were analyzed using Review Manager 5.4.4 and subgroup analyses were applied.

Results: A total of 11 studies were eligible for meta-analysis. The synthetic data suggested that anti-reflux anastomosis and direct anastomosis were comparable in terms of RFn impairment (odds ratio (OR) = 1.69; 95% confidence interval (CI): 0.18-15.6; p = 0.65, I2 = 69%) and pyelonephritis (OR = 1.13; 95% CI: 0.65-1.99; p = 0.66, I2 = 1%) without significant difference in each group statistically. The pooled study data showed a significantly higher incidence of UEAS (OR = 2.84; 95% CI: 1.75-4.61, p < 0.0001, I2 = 50%) and a lower incidence of vesicoureteral reflux (OR = 0.24; 95% CI: 0.10-0.59; p = 0.002, I2 = 75%) in anti-reflux anastomosis compared to direct anastomosis. In subgroup analysis, anti-reflux anastomosis was more likely to result in UEAS than direct anastomosis, especially when ureteral stent was removed within 14 days.

Conclusion: Although meta-analysis showed that overall incidence of vesicoureteral reflux was higher with direct anastomosis than anti-reflux anastomosis, the rate of vesicoureteral reflux was not directly related to impairment of RFn. The anti-reflux mechanism of ONB was positively associated with a higher incidence of significant UEAS compared to the direct approach, which can lead to kidney damage and an increased risk of secondary surgical procedures.
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http://dx.doi.org/10.1159/000525017DOI Listing
July 2022

Combined inhibition of BADSer99 phosphorylation and PARP ablates models of recurrent ovarian carcinoma.

Commun Med (Lond) 2022 2;2:82. Epub 2022 Jul 2.

Tsinghua Berkeley Shenzhen Institute, Tsinghua Shenzhen International Graduate School, Tsinghua University, Shenzhen, 518055 PR China.

Background: Poly (ADP-ribose) polymerase inhibitors (PARPis) have been approved for the treatment of recurrent epithelial ovarian cancer (EOC), regardless of status or homologous recombination repair deficiency. However, the low response of platinum-resistant EOC, the emergence of resistance in deficient cancer, and therapy-associated toxicities in patients limit the clinical utility of PARPis in recurrent EOC.

Methods: The association of phosphorylated (p) BADS99 with clinicopathological parameters and survival outcomes in an EOC cohort was assessed by immunohistochemistry. The therapeutic synergy, and mechanisms thereof, between a pBADS99 inhibitor and PARPis in EOC was determined in vitro and in vivo using cell line and patient-derived models.

Results: A positive correlation between pBADS99 in EOC with higher disease stage and poorer survival is observed. Increased pBADS99 in EOC cells is significantly associated with -deficiency and decreased Cisplatin or Olaparib sensitivity. Pharmacological inhibition of pBADS99 synergizes with PARPis to enhance PARPi IC and decreases survival, foci formation, and growth in ex vivo culture of EOC cells and patient-derived organoids (PDOs). Combined inhibition of pBADS99 and PARP in EOC cells or PDOs enhances DNA damage but impairs PARPi stimulated DNA repair with a consequent increase in apoptosis. Inhibition of BADS99 phosphorylation synergizes with Olaparib to suppress the xenograft growth of platinum-sensitive and resistant EOC. Combined pBADS99-PARP inhibition produces a complete response in a PDX derived from a patient with metastatic and chemoresistant EOC.

Conclusions: A rational and efficacious combination strategy involving combined inhibition of pBADS99 and PARP for the treatment of recurrent EOC is presented.
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http://dx.doi.org/10.1038/s43856-022-00142-3DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9250505PMC
July 2022

Functionalized Erythrocyte Membrane-Coated Nanoparticles for the Treatment of -Induced Sepsis.

Front Microbiol 2022 16;13:901979. Epub 2022 Jun 16.

Department of Laboratory Medicine, Wuxi Fifth People's Hospital Affiliated to Nantong University, Wuxi, China.

Sepsis is a systemic inflammatory response syndrome caused by infection, with high incidence and mortality. Therefore, it is necessary to carry out an effective anti-infection treatment. In this work, we designed and synthesized red blood cell (RBC) membrane-coated PLGA nanoparticles named γ3-RBCNPs, which target the highly expressed intercellular adhesion molecule-1 (ICAM-1) at the site of infection through the γ3 peptide on its surface and kill the through ciprofloxacin encapsulated in its core. In addition, the homogenous RBC membrane coated on the surface of the nanoparticles helps them avoid immune surveillance and prolong the circulation time of the drug in the body. We found that the γ3-RBCNPs target human umbilical vein endothelial cells (HUVECs) activated by TNF-α and the infected lung of mice in the sepsis model very well. evaluation suggested that γ3-RBCNPs have a low risk of acute hemolysis and are less likely to be engulfed by macrophages. evaluation showed that γ3-RBCNPs has a long half-life and good bio-safety. More importantly, we confirmed that γ3-RBCNPs have the good antibacterial and anti-infection ability and . Our research provides a new strategy for the nano-drug treatment of Klebsiella pneumoniae-induced sepsis.
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http://dx.doi.org/10.3389/fmicb.2022.901979DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9244542PMC
June 2022

Ultrafine cobalt molybdenum phosphide nanoparticles embedded in crosslinked nitrogen-doped carbon nanofiber as efficient bifunctional catalyst for overall water splitting.

J Colloid Interface Sci 2022 Nov 24;625:956-964. Epub 2022 Jun 24.

State Key Laboratory of Chemistry and Utilization of Carbon Based Energy Resources, Urumqi, 830017 Xinjiang, PR China; College of Chemistry, Xinjiang University, Urumqi, 830017 Xinjiang, PR China.

As a kind of high-performance and cost-efficient electrocatalyst in water splitting, the transition bimetal phosphides exhibit a promising prospect. Here, the composite of cobalt molybdenum phosphide nanoparticles embedded in crosslinked nitrogen-doped carbon nanofiber ([email protected]) has been synthesized via an electrospinning process and pyrolysis treatment. As an effective hydrogen evolution reaction (HER) and oxygen evolution reaction (OER) catalyst, the [email protected] only requires the overpotentials of 81 mV and 219 mV at the current densities of 10 mA cm and 20 mA cm, respectively. Moreover, the water electrolyzer with the [email protected] as the cathode and anode catalysts requires a cell voltage of 1.59 V to reach a current density of 10 mA cm. The [email protected] also achieves the excellent stability up to 24 h for HER, OER and overall water spitting in 1.0 M KOH. The excellent catalytic activity of the [email protected] is benefits from the synergistic effect between components and the crosslinked structure of carbon nanofiber. Thus, the research provides a promising method for preparing carbon-based TMPs materials towards electrocatalysis.
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http://dx.doi.org/10.1016/j.jcis.2022.06.093DOI Listing
November 2022

Characterization of a Novel Carbapenem-Resistant Klebsiella michiganensis Strain Coharboring the bla, bla, bla, qnrS, and aac(6')-Ib-cr Genes.

Curr Microbiol 2022 Jun 25;79(8):228. Epub 2022 Jun 25.

The Fifth Affiliated Hospital of Wenzhou Medical University, Lishui, Zhejiang, China.

Carbapenem-resistant Klebsiella michiganensis (CRKM) and Klebsiella oxytoca (CRKO) strains have occasionally been reported to cause severe infections. However, SIM-producing K. michiganensis strains have never been described. In this study, we phenotypically and genetically characterized 6 CRKM and CRKO strains isolated over the past 10 years at a Chinese tertiary hospital. All six strains were positive for the mCIM test, and five were positive for the MBL test. Carbapenemase-encoding genes (bla, bla, bla, bla, bla, bla, bla, and bla) and another 12 resistance genes were screened by PCR, and bla, bla, and bla were identified in five strains. However, the CRKM strain KM41, which was resistant to IPM and MEM with minimum inhibitory concentrations (MICs) of 4 µg/ml and 16 µg/ml, respectively, had positive mCIM and MBL results but lacked the eight carbapenemase-encoding genes. Whole-genome sequencing of the KM41 strain revealed more than 20 drug resistance genes; in particular, bla, bla, bla, qnrS, aac(6')-Ib-cr, aadA17, and aar-3 were found to be located in a single plasmid. To the best of our knowledge, this is the first description of a K. michiganensis strain coharboring bla, bla, bla, qnrS, and aac(6')-Ib-cr in China.
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http://dx.doi.org/10.1007/s00284-022-02920-7DOI Listing
June 2022

Effects of Mixed Decomposition of var. and Litter on Microbial Diversity.

Microorganisms 2022 May 28;10(6). Epub 2022 May 28.

Institute of Modern Agricultural Research, Dalian University, Dalian 116622, China.

var. is widely planted in China as a windbreak and sand fixation tree. To improve the current situation of large-scale declines of forested areas planted as var. monocultures, we investigated the biological and microbial effects of stand establishment using mixed tree species. The interactions during the mixed decomposition of the litter and leaves of different tree species are an important indicator in determining the relationships among species. In this experiment, a method of simulating the mixed decomposition of var. and litter under var. forest was used to determine the total C, total N, and total P contents in the leaf litter, and the microbial structures were determined by using Illumina MiSeq high-throughput sequencing. It was found that with samples with different proportions of var. and litters, the decomposition rate of var. × litter was significantly higher than that of the pure var. forest, and the microbial community and composition diversity of litter in a pure var. forest could be significantly improved. The possibility of using as a mixed tree species to address the declines of pure var. forest was verified to provide guidance for pure var. forests by introducing tree species with coordinated interspecific relationships and creating a mixed forest.
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http://dx.doi.org/10.3390/microorganisms10061117DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9229243PMC
May 2022

Vasa Is a Potential Germ Cell Marker in Leopard Coral Grouper ().

Genes (Basel) 2022 Jun 16;13(6). Epub 2022 Jun 16.

Key Laboratory of Marine Genetics and Breeding (Ministry of Education), College of Marine Life Sciences, Ocean University of China, Qingdao 266003, China.

Vasa (, DEAD box polypeptide 4), an extremely specific marker of germ cells in vivo, is an ATP-dependent RNA helicase that plays an essential role in germ cell development and gametogenesis. However, the expression and function information about this gene in groupers remains lacking. Here, homolog termed was isolated and identified as a putative germ cell marker in the leopard coral grouper, (). Results indicated that contained 17 exons in the genomic sequence and 9 conserved motifs of the DEAD-box protein by sequence analysis. The sequence comparison, phylogenetic analyses and synteny analyses showed that was homologous with other teleosts. Additionally, the expression of was significantly higher in gonads than in other tissues in adult individuals ( < 0.05). Further, the distribution of revealed that it was only expressed in the germ cells, such as spermatids, germline stem cells and oocytes at different stages, and could not be detected in the somatic cells of gonads. The current study verified that the gene is a valuable molecular marker of germ cells in leopard coral grouper, which potentially plays an important role in investigating the genesis and development of teleost germ cells.
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http://dx.doi.org/10.3390/genes13061077DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9222667PMC
June 2022

A deep learning-based hybrid artificial intelligence model for the detection and severity assessment of vitiligo lesions.

Ann Transl Med 2022 May;10(10):590

Department of Cosmetic Laser Surgery, Hospital for Skin Disease and Institute of Dermatology, Chinese Academy of Medical Sciences (CAMS) & Peking Union Medical College, Nanjing, China.

Background: We aimed to establish and validate a deep learning-based hybrid artificial intelligence (AI) model for the objective morphometric and colorimetric assessment of vitiligo lesions.

Methods: Two main datasets containing curated images of vitiligo lesions from Chinese patients (Fitzpatrick skin types III or IV) were established, including one with 2,720 images for lesion localization study and the other with 1,262 images for lesion segmentation study. Besides, an additional test set containing 145 images of vitiligo lesions from other Fitzpatrick skin types (I, II, or V) was also generated. A 3-stage hybrid model was constructed. YOLO v3 (You Only Look Once, v3) architecture was trained and validated to classify and localize vitiligo lesions, with sensitivity and error rate as primary performance outcomes. Then a segmentation study comparing 3 deep convolutional neural networks (DCNNs), Pyramid Scene Parsing Network (PSPNet), UNet, and UNet++, was carried out based on the Jaccard index (JI). The architecture with the best performance was integrated into the model. Three add-on metrics, namely VAreaA, VAreaR, and VColor were finally developed to measure absolute, relative size changes and pigmentation, respectively. Agreement between the AI model and dermatologist evaluators were assessed.

Results: The sensitivity of the YOLO v3 architecture to detect vitiligo lesions was 92.91% with an error rate of 14.98%. The UNet++ architecture outperformed the others in the segmentation study (JI, 0.79) and was integrated into the model. On the additional test set, however, the model achieved a lower detection sensitivity (72.41%) and a lower segmentation score (JI, 0.69). With respect to size changes, no difference was observed between the AI model, trained dermatologists (W=0.812, P<0.05), and Photoshop analysis (P=0.075, P=0.212 respectively), which all displayed good concordance.

Conclusions: We developed a novel, convenient, objective, and quantitative deep learning-based hybrid model which simultaneously evaluated both morphometric and colorimetric vitiligo lesions from patients with Fitzpatrick skin types III or IV, rendering it suitable for the assessment of severity of vitiligo lesions in Asians in both clinic and research scenarios. More work is also warranted for its use in other ethnic skin groups.
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http://dx.doi.org/10.21037/atm-22-1738DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9201159PMC
May 2022

Screening of periodontitis-related genes and immune cells based on an integrated bioinformatics analysis.

Ann Transl Med 2022 May;10(10):558

School of Stomatology, Youjiang Medical University for Nationalities, Baise, China.

Background: Periodontitis is a serious health issue; however, there are few reports on periodontitis and immune related. The study sought to analyze differences in gene expression between periodontitis patients before and after treatment using bioinformatics techniques.

Methods: The GSE6751 data set was screened for differentially expressed genes (DEGs) and immune-related genes using the Gene Expression Omnibus (GEO) database, a gene expression data analysis tool. Enrichment, protein-protein interaction (PPI) network construction, and immune cell correlation analyses were also performed on these genes.

Results: A total of 327 DEGs in periodontitis were identified, including 149 upregulated genes, 178 downregulated genes, and 27 immune-related genes. The immune-related DEGs were mainly involved in granulocyte chemotaxis, granulocyte migration, leukocyte chemotaxis, cytokine receptor binding, Toll-like receptor (TLR) binding, the phosphatidylinositol-3-kinase and protein kinase b signaling pathway, viral protein interactions with cytokines and cytokine receptors, the Ras-proximate-1 signaling pathway, the Ras signaling pathway, natural killer cell mediated cytotoxicity, and immune response related pathways. and were the key core genes in the constructed PPI network.

Conclusions: The pathogenesis of periodontitis is closely related to changes in the expression of genes, such as and , among which IFNG and TLR2 are potential therapeutic targets for periodontitis.
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http://dx.doi.org/10.21037/atm-22-1592DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9201185PMC
May 2022

Fabrication of uniform lignin nanoparticles with tunable size for potential wound healing application.

Int J Biol Macromol 2022 Aug 14;214:170-180. Epub 2022 Jun 14.

Liaoning Key Laboratory of Lignocellulose Chemistry and Biomaterials, Dalian Polytechnic University, Dalian, Liaoning 116034, China.

The construction of lignin nanoparticles (LNPs) with both lignin properties and nanomaterial properties through controlling the morphologies and structures of lignin is one of the effective ways to realize its application in the field of biomedicine. Firstly, the morphology and chemical structure of LNPs were studied in detailed. The results showed that the chemical structural characteristics of LNPs had not changed significantly and its morphology was more regular shape and narrower size distribution (50-350 nm). Besides, LNPs also exhibited excellent water dispersion stability and high negative zeta potential. Subsequently, LNPs as wound dressings had good antioxidant property, excellent adsorption capacity of protein, outstanding bactericidal activity and remarkable biocompatibility, suggesting that LNPs did not interfere with cell proliferation during wound healing. Finally, the in vivo results of mouse wounds further illustrated that treatment of wounded skin wounds with LNPs enhanced its effective healing. After 15 days, as compared with the untreated control and original lignin (OL) groups, the wounds treated of LNPs was completely closed and granulation tissue formation was advanced. Overall, this study can be a good method for high-value applications of LNPs, and highlighting the advantages of using lignin as medical adjuvant nanomaterials to accelerate wound healing.
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http://dx.doi.org/10.1016/j.ijbiomac.2022.06.066DOI Listing
August 2022

Temperature-insensitive polarimetric vibration sensor.

Opt Lett 2022 Jun;47(11):2714-2717

Vibration measurement is a frequent measurement requirement in a number of areas. Optical vibration sensors have many advantages over electrical counterparts. A common approach is to optically detect the vibration induced mechanical movement of a cantilever. Nevertheless, their practical applications are hindered by the cross-sensitivity of temperature and dynamic instability of the mechanical structure, which lead to unreliable vibration measurements. Here, we demonstrate a temperature insensitive vibration sensor that involves an enclosed suspended cantilever integrated with a readout fiber, providing in-line measurement of vibration. The cantilever is fabricated from a highly birefringent photonic crystal fiber by chemical etching and fused to a single-polarization fiber. Mechanical vibration induced periodic bending of the cantilever can significantly modify the state of polarization of the light that propagates along the photonic crystal fiber. The single-polarization fiber finally converts the state of polarization fluctuation into the change of output optical power. Therefore, the vibration could be demodulated by monitoring the output power of the proposed structure. Due to the special design of the structure, the polarization fluctuation induced by a variation of the ambient temperature can be significantly suppressed. The sensor has a linear response over the frequency range of 5 Hz to 5 kHz with a maximum signal-to-noise ratio of 60 dB and is nearly temperature independent.
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http://dx.doi.org/10.1364/OL.456245DOI Listing
June 2022

An efficient and sensitive method on the identification of unsaturated fatty acids in biosamples: Total lipid extract from bovine liver as a case study.

J Chromatogr A 2022 Jul 25;1675:463176. Epub 2022 May 25.

State Key Laboratory of Component-based Chinese Medicine, Tianjin University of Traditional Chinese Medicine, 10 Poyanghu Road, Jinghai district, Tianjin 301617, PR China; Tianjin Key Laboratory of TCM Chemistry and Analysis, Tianjin University of Traditional Chinese Medicine, 10 Poyanghu Road, Jinghai district, Tianjin 301617, PR China. Electronic address:

Unsaturated fatty acids (UFAs) are fatty acids with one or more carbon-carbon double bonds (C=C). They are building blocks of structurally complex lipids. According to the position of C=C bond in the UFAs, there are multiple isomers with different physiological activities. Therefore, an accurate understanding of UFAs is essential. Combining Paternò-Büchi (PB) reaction with tandem mass spectrometry (MS/MS) analysis has been successfully used to identify unsaturated lipids in bio-samples. However, there are still some challenges, such as low reactive rate and low MS response of PB products. In this study, 3-pyridinecarboxaldehyde (3-PYA) was selected as an efficient PB reagent for the first time. According to the nitrogen rule, introduction of nitrogen did not only make the target MS and MS/MS ions to be easily identified but also increase the ionization efficiency significantly. The developed method in this study demonstrated greater efficiency and sensitivity for the identification of UFAs in total lipid extracts from bovine liver compared with other commonly used PB reagents.
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http://dx.doi.org/10.1016/j.chroma.2022.463176DOI Listing
July 2022

Functional Ovarian Cysts in Artificial Frozen-Thawed Embryo Transfer Cycles With Depot Gonadotropin-Releasing Hormone Agonist.

Front Endocrinol (Lausanne) 2022 29;13:828993. Epub 2022 Apr 29.

Department of Reproductive Medicine, Women's Hospital of Nanjing Medical University, Nanjing Maternity and Child Health Care Hospital, Nanjing, China.

Objectives: To investigate the incidence of functional ovarian cysts, its influence on clinical rates, and proper management after depot gonadotropin-releasing hormone (GnRH) agonist pretreatment in artificial frozen-thawed embryo transfer cycles (AC-FET).

Methods: This retrospective cohort study involved 3375 AC-FET cycles with follicular-phase depot GnRH agonist administration between January 2017 and December 2020. Subjects were divided into a study group (cycles with cyst formation) and a control group (cycles without cyst formation). The study group was matched by propensity scoring matching with the control group at a ratio of 1:2. For patients with ovarian cyst formation, two major managements were used: a conservative approach (i.e., expectant treatment) and a drug approach (i.e., continued agonist administration). The primary outcome was live birth rate (LBR).

Results: The incidence of functional ovarian cysts following pituitary downregulation is 10.1% (341/3375). The study group exhibited a LBR similar to the control group (54.5% . 50.1%, adjusted odds ratio [aOR] 1.17, 95% confidence interval [CI] 0.88-1.56, 0.274). Patients with a lower body mass index and anti-Müllerian hormone, and a higher basal estradiol level were more susceptible to developing functional ovarian cysts. The LBR decreased after the drug approach compared with the conservative approach, but not significantly (aOR 0.63, 95% CI 0.35-1.14, 0.125). Following the conservative approach, cycles arrived at live births had a significantly shorter duration from the detection of functional cysts to the start of endometrium preparation (15.7 ± 5.1 days . 17.4 ± 5.3 days, = 0.009) and a significantly higher proportion of ovarian cysts on the initial day of exogenous hormone supplementation (51.4% . 30.3%, = 0.001). After controlling for all confounders, the differences remained statistically significant.

Conclusions: It is unnecessary to cancel cycles that experience functional ovarian cyst formation. Conservative management and further agonist suppression protocol had similar pregnancy rates. However, a conservative approach was recommended due to its lower cost and fewer side effects. Our findings support a shorter waiting period when choosing the conservative protocol.
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http://dx.doi.org/10.3389/fendo.2022.828993DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9102377PMC
May 2022

Computational Insight into Biotransformation Profiles of Organophosphorus Flame Retardants to Their Diester Metabolites by Cytochrome P450.

Molecules 2022 Apr 28;27(9). Epub 2022 Apr 28.

Institute of Ageing Research, School of Medicine, Hangzhou Normal University, Hangzhou 311121, China.

Biotransformation of organophosphorus flame retardants (OPFRs) mediated by cytochrome P450 enzymes (CYPs) has a potential correlation with their toxicological effects on humans. In this work, we employed five typical OPFRs including tris(1,3-dichloro-2-propyl) phosphate (TDCIPP), tris(1-chloro-2-propyl) phosphate (TCIPP), tri(2-chloroethyl) phosphate (TCEP), triethyl phosphate (TEP), and 2-ethylhexyl diphenyl phosphate (EHDPHP), and performed density functional theory (DFT) calculations to clarify the CYP-catalyzed biotransformation of five OPFRs to their diester metabolites. The DFT results show that the reaction mechanism consists of Cα-hydroxylation and O-dealkylation steps, and the biotransformation activities of five OPFRs may follow the order of TCEP ≈ TEP ≈ EHDPHP > TCIPP > TDCIPP. We further performed molecular dynamics (MD) simulations to unravel the binding interactions of five OPFRs in the CYP3A4 isoform. Binding mode analyses demonstrate that CYP3A4-mediated metabolism of TDCIPP, TCIPP, TCEP, and TEP can produce the diester metabolites, while EHDPHP metabolism may generate -hydroxyEHDPHP as the primary metabolite. Moreover, the EHDPHP and TDCIPP have higher binding potential to CYP3A4 than TCIPP, TCEP, and TEP. This work reports the biotransformation profiles and binding features of five OPFRs in CYP, which can provide meaningful clues for the further studies of the metabolic fates of OPFRs and toxicological effects associated with the relevant metabolites.
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http://dx.doi.org/10.3390/molecules27092799DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9102461PMC
April 2022

Hydroxyacid Oxidase 2 (HAO2) Inhibits the Tumorigenicity of Hepatocellular Carcinoma and Is Negatively Regulated by miR-615-5p.

J Immunol Res 2022 28;2022:5003930. Epub 2022 Apr 28.

Department of Gastroenterology, Zhengzhou University People's Hospital, Henan Provincial People's Hospital, Zhengzhou, Henan 450003, China.

Background: Hepatocellular carcinoma (HCC) is the sixth most common kind of cancer worldwide and the third leading cause of cancer mortality. Although a few studies have shown that hydroxyacid oxidase 2 (HAO2) may prevent HCC development, the molecular mechanism is unclear.

Methods: We examined the levels of HAO2 expression in 23 pairs of HCC/paracancerous tissues by quantitative real-time polymerase chain reaction (qRT-PCR) and evaluated HAO2's expression in The Cancer Genome Atlas (TCGA) database. Furthermore, we examined the biological activity of HAO2 utilizing cell-based functional assays. Additionally, we evaluated the relationship between miR-615-5p and HAO2 in Hep3B cells using a dual-luciferase reporter system and assessed the downstream regulatory mechanisms of miR-615-5p on HAO2. Finally, the nude mice tumor formation experiment was used to determine the impact of HAO2 on the tumorigenicity of HCC cells.

Results: HAO2 expression was considerably underexpression in HCC tissues and cells, and patients with low HAO2 expression had poorer disease-free survival. Inhibition of cell proliferation, migration, and invasion was observed when HAO2 was overexpressed. miR-615-5p had a negative relation with HAO2, and miR-615-5p restored HAO2's biological activity in HCC cells. Additionally, the tumor volume and weight were considerably reduced in the OV-HAO2 group compared to the OV-NC group.

Conclusion: HAO2 was found to be underexpressed in HCC tissues and cells, and HAO2 overexpression inhibited HCC cell motility, which was negatively regulated by miR-615-5p. Exogenous expression of HAO2 reduced the tumorigenicity of HCC cells in vivo in nude mice.
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http://dx.doi.org/10.1155/2022/5003930DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9071856PMC
May 2022

Organo-mineral complexes alter bacterial composition and induce carbon and nitrogen cycling in the rhizosphere.

Sci Total Environ 2022 Aug 4;836:155671. Epub 2022 May 4.

School of Environmental Science and Engineering, Tianjin University, Weijin Road, Tianjin 300072, China. Electronic address:

It is widely thought that organo-mineral complexes (OMCs) stabilize organic matter via mineral adsorption. Recent studies have demonstrated that root exudates can activate OMCs, but the influence of OMCs on plant rhizosphere, which is among the most active areas for microbes, has not been thoroughly researched. In this study, a pot experiment using Brassica napus was conducted to investigate the effects of OMCs on plant rhizosphere. The result showed that OMC addition significantly promoted the growth of B. napus compared to the prevalent fertilization (PF, chemical fertilizer + chicken compost) treatment. Specifically, OMC addition increased the relative abundance (RA) of nitrogen-fixing bacteria and the bacterial α-diversity, and the operational taxonomic unit (OTU) group with RA > 0.5% in the OMC-treated rhizosphere was the result of a deterministic assembly process with homogeneous selection. Gene abundance related to nitrogen cycling and the soil chemical analysis demonstrated that the OMC-altered bacterial community induced nitrogen fixation and converted nitrate to ammonium. The upregulated carbon sequestration pathway genes and the increased soil microbial biomass carbon (23.68%) demonstrated that the bacterial-induced carbon storage in the rhizosphere was activated. This study shows that the addition of OMCs can influence the biogeochemical carbon and nitrogen cycling via regulating microorganisms in the rhizosphere. The findings provide fresh insights into the effects of OMCs on the biogeochemical cycling of important elements and suggest a promising strategy for improving soil productivity.
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http://dx.doi.org/10.1016/j.scitotenv.2022.155671DOI Listing
August 2022

A miniature fiber-optic microphone based on plano-concave micro-interferometer.

Rev Sci Instrum 2022 Apr;93(4):045001

State Key Laboratory of Electrical Insulation and Power Equipment, School of Electrical Engineering, Xi'an Jiaotong University, Xi'an 710049, China.

The sensitive detection of sound waves is essential for a variety of applications. In this work, we propose a miniature diaphragm-free fiber-optic microphone based on a plano-concave optical micro-interferometer. A solid plano-concave micro-interferometer is formed at the end of a cleaved fiber by depositing a tiny volume of liquefied glass. Sound wave induced periodic variation of pressure can significantly modify the refractive index of the plano-concave glass due to the elasto-optic effect, and then, the phase difference between two interferometric beams will be remarkably changed accordingly. The interferometer finally converts the fluctuation of the phase difference into the change in the output optical power. Consequently, the sound wave can be demodulated by detecting the output power of the microphone. The experimental results show that the proposed microphone has the ability to detect sound waves in the whole audible range and almost omnidirectional. The noise-limited minimum detectable sound pressure is around 12 µPa/Hz. In addition, the human voice detection test shows that the performance of our microphone is competitive with the most advanced commercial device. The structure is stable without any movable mechanical parts, and the size is as small as 0.25 mm, which makes the proposed microphone an attractive alternative to the conventional one for sound wave detection.
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http://dx.doi.org/10.1063/5.0084559DOI Listing
April 2022

Design, synthesis and applications of NIR-emissive scaffolds of diketopyrrolopyrrole-aza-BODIPY hybrids.

Chem Commun (Camb) 2022 May 17;58(40):5996-5999. Epub 2022 May 17.

Key Laboratory of Functional Molecular Engineering of Guangdong Province, School of Chemistry and Chemical Engineering, South China University of Technology, Guangzhou, 510641, China.

Herein, we report a facile synthetic method towards diketopyrrolopyrrole-aza-BODIPY hybrids by a two-step strategy. The three resulting fluorophores (LAB-TH4 ∼ LAB-TH6) showed absorption maxima up to 637 nm with molar extinction coefficients () up to 32 300 M cm and emission maxima at 661 nm with a fluorescence quantum yield of 25.2% in solution. Due to good biocompatibility and strong ROS generation ability, they are useful fluorophores for bioimaging and photodynamic therapy (PDT). In addition, as a useful scaffold, a novel NIR-emissive conjugated polymer can be constructed by further bromination and Suzuki coupling polymerization.
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http://dx.doi.org/10.1039/d2cc00774fDOI Listing
May 2022

Differentially expressed mRNAs and their upstream miR-491-5p in patients with coronary atherosclerosis as well as the function of miR-491-5p in vascular smooth muscle cells.

Korean J Physiol Pharmacol 2022 May;26(3):183-193

Department of Cardiovascular Surgery, The Affiliated Huaian No. 1 People's Hospital of Nanjing Medical University, Huaian, Jiangsu 223300, China.

MicroRNAs (miRNAs) regulate gene expression and are biomarkers for coronary atherosclerosis (AS). A novel miRNA-mRNA regulation network of coronary AS still needs to be disclosed. The aim of this study was to analyze potential mRNAs in coronary AS patients and the role of their upstream miR-491-5p in vascular smooth muscle cells (VSMCs). We first confirmed top ten mRNAs according to the analysis from Gene Expression Omnibus database (GSE132651) and examined the expression levels of them in the plaques and serum from AS patients. Five mRNAs (UBE2G2, SLC16A3, POLR2C, PNO1, and AMDHD2) presented significantly abnormal expression in both plaques and serum from AS patients, compared with that in the control groups. Subsequently, they were predicted to be targeted by 11 miRNAs by bioinformatics analysis. Among all the potential upstream miRNAs, only miR-491-5p was abnormally expressed in the plaques and serum from AS patients. Notably, miR-491-5p overexpression inhibited viability and migration, and significantly increased the expression of contractile markers (α-SMA, calponin, SM22α, and smoothelin) in VSMCs. While silencing miR-491-5p promoted viability and migration, and significantly suppressed the expression of α-SMA, calponin, SM22α, and smoothelin. Overall, miR-491-5p targeted UBE2G2, SLC16A3, and PNO1 and regulated the dysfunctions in VSMCs.
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http://dx.doi.org/10.4196/kjpp.2022.26.3.183DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9046892PMC
May 2022

Miniature Fourier transform spectrometer based on a fiber-tip interferometer.

Opt Express 2022 Apr;30(9):15134-15148

The miniaturization of spectrometers have attracted much attention owning to the demand for portable or in situ spectral analysis in a wide variety of fields, but it is a great challenge to push them into practical applications due to high cost, complicated configuration, and sensitivity to external disturbance. We report on a miniature Fourier transform (FT) spectrometer based on fiber-tip Fizeau interferometer. Hand pulling or any other types of force can be used to drive optical path difference (OPD) scan. Interferences are monitored as a function of time by two photodetectors, one is used to detect the whole interferogram while the other to measure single-wavelength interferogram. In this design, the instantaneous interference intensity as well as OPD can be obtained in an accurate way so that the exact spatial interferogram of the incident spectrum can be worked out. Consequently, the incident spectrum can be retrieved by FT method. A resolution of 7.69 cm in the wavelength range of 1400 nm ∼ 1700 nm is achieved. Experimental results show that the performance of our device is comparable to the commercial benchtop spectrometer. Our device is independent of the complicated fabrication procedures, easy of usage, and cost effective. We envision that the proposed design will inspire a new concept for constructing simple and cheap spectrometers that is well suited for practical applications.
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http://dx.doi.org/10.1364/OE.456320DOI Listing
April 2022

A type II heterojunction α-FeO/g-CN for the heterogeneous photo-Fenton degradation of phenol.

RSC Adv 2022 Mar 16;12(14):8300-8309. Epub 2022 Mar 16.

National Engineering Research Center of Coal Preparation and Purification, China University of Mining and Technology Xuzhou 221000 Jiangsu China

The heterogeneous photo-Fenton reaction is an effective method of chemical oxidation to remove phenol in wastewater with environmental friendliness and sustainability. Herein, the composite α-FeO/g-CN, as a catalyst of the heterogeneous photo-Fenton reaction, has been synthesized by hydrothermal-calcination method using the abundant and low-cost FeCl·6HO and g-CN as raw materials. The influence of the annealing temperature during calcination was also investigated. The UV-Vis diffuse reflectance spectra of samples show that the composite α-FeO/g-CN possesses the widest light response range. Furthermore, the transient photocurrent response curves demonstrated the strongest intensity of α-FeO/g-CN. The annealed α-FeO/g-CN is indicative of the highest degradation efficiency in all samples due to the improvement of the charge transfer ability caused by the tight heterojunction structure. The results of the scavenger trapping experiments show that the hydroxyl radical was the main active species in degradation. Based on experimental results, a type II heterojunction should be built in the composite α-FeO/g-CN, driving the photoelectrons transfer and migration by internal electronic field. This work provides a facile and new method to synthesize α-FeO/g-CN as an effective heterogeneous photo-Fenton catalyst for environmental remediation.
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http://dx.doi.org/10.1039/d1ra09282kDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8984958PMC
March 2022

A Comparative Study on Sedation Efficacy between General and Regional Anesthesia with Dexmedetomidine in Patients under Maxillofacial Surgery.

Curr Drug Metab 2022 Apr 13. Epub 2022 Apr 13.

Department of Anesthesiology, Shaanxi Provincial People\'s Hospital, Xi\'an 710068, China.

Background And Objective: Securing the airway in the surgery of maxillofacial disorders and traumas is fundamental during the operation. The present study aims to investigate the beneficial sedative effects of dexmedetomidine (DEX) in patients who underwent maxillofacial surgery with regional anesthesia compare to general anesthesia.

Methods: Fifty patients, aged 20-45 years old, were randomly divided into two groups of regional anesthesia (RA) and general anesthesia (GA) (each n=25). The group RA received regional block with sedation (DEX: 1 µg/kg infused over 10 min followed by the maintenance dose of 0.5 µg/kg/h) and the group GA underwent general anesthesia (DEX: 0.1 µg/kg/min over 10 min followed by 0.4-0.7 µg/kg/h). Postoperative pain scores, anesthesia outcomes, hemodynamic parameters, the time of the post-anesthesia care unit (PACU) discharge and intra and postoperative complications were comparatively assessed in both groups.

Results: The baseline characteristics of the patients (age, gender, BMI and ASA physical status) showed no differences between two groups (P>0.05). Although the duration of surgery and recovery time showed no differences between the groups, duration of anesthesia and extubation time was remarkably lower in the RA group than the GA group (P<0.01). Administration of nerve blocks demonstrated less pain and longer sleep time in the postoperative phase as compared to the GA group. Heart rate and mean arterial blood pressure were significantly less in the RA group at the end of the loading dose of DEX and incision time (P<0.05). SpO2, respiration rate and Ramsay sedation scale did not exhibit any significant differences between the two groups at all-time points (P>0.05). No significant differences were observed with regard to the adverse events between the two groups (P>0.05).

Conclusions: Although our findings revealed that both methods are suitable and safe methods for maxillofacial surgery, the outcomes of anesthesia with regional block and sedation including less pain in the postoperative phase, shorter extubation time and earlier discharge from the PACU demonstrated that this method is more reliable for maxillofacial surgery. Further controlled studies are needed to compare the effectiveness and safety profiles of two RA and GA techniques and also to compare DEX with other anesthetic agents to achieve optimum outcomes in the maxillofacial surgeries.
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http://dx.doi.org/10.2174/1389200223666220413113412DOI Listing
April 2022
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