Publications by authors named "Hui Chen"

3,794 Publications

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Early risk factors for extrapulmonary organ injury in adult COVID-19 patients.

Ann Transl Med 2021 Apr;9(8):701

Department of Intensive Care Medicine, The First Affiliated Hospital of Soochow University, Suzhou, China.

Background: The novel 2019 coronavirus (COVID-19) has caused a global pandemic, and often leads to extrapulmonary organ injury. However, the risk factors for extrapulmonary organ injury are still unclear. We aim to explore the risk factors for extrapulmonary organ injury and the association between extrapulmonary organ injury and the prognosis in COVID-19 patients.

Methods: We implemented a single-center, retrospective, observational study, in which a total of 349 confirmed COVID-19 patients admitted to Tongji Hospital from January 25, 2020, to February 25, 2020, were enrolled. We collected demographic, clinical, laboratory, and treatment data from electronic medical records. Potential risk factors for extrapulmonary organ injury of COVID-19 patients were analyzed by a multivariable binary logistic model, and multivariable Cox proportional hazards regression model was used for survival analysis in the patients with extrapulmonary organ injury.

Results: The average age of the included patients was 61.73±14.64 years. In the final logistic model, variables including aged 60 or older [odds ratio (OR) 1.826, 95% confidence interval (CI): 1.060-3.142], acute respiratory distress syndrome (ARDS) (OR 2.748, 95% CI: 1.051-7.185), lymphocytes count lower than 1.1×10/L (OR 0.478, 95% CI: 0.240-0.949), level of interleukin-6 (IL-6) greater than 7 pg/mL (OR 1.664, 95% CI: 1.005-2.751) and D-Dimer greater than 0.5 μg/mL (OR 2.190, 95% CI: 1.176-4.084) were significantly associated with the extrapulmonary organ injury. Kaplan-Meier curve and log-rank test showed that the probabilities of survival for patients with extrapulmonary organ injury were significantly lower than those without extrapulmonary organ injury. Multivariate Cox proportional hazards model showed that only myocardial injury (P=0.000, HR: 5.068, 95% CI: 2.728-9.417) and circulatory system injury (P=0.000, HR: 4.076, 95% CI: 2.216-7.498) were the independent factors associated with COVID-19 patients' poor prognosis.

Conclusions: Older age, lymphocytopenia, high level of D-Dimer and IL-6, and the severity of lung injury were the high-risk factors of extrapulmonary organ injury in COVID-19 patients. Myocardial and circulatory system injury were the most important risk factors related to poor outcomes of COVID-19 patients. It may help clinicians to identify extrapulmonary organ injury early and initiate appropriate treatment.
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http://dx.doi.org/10.21037/atm-21-1561DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8106092PMC
April 2021

Visual Deprivation Retards the Maturation of Dendritic Fields and Receptive Fields of Mouse Retinal Ganglion Cells.

Front Cell Neurosci 2021 27;15:640421. Epub 2021 Apr 27.

Department of Ophthalmology and Visual Science, University of Utah School of Medicine, Salt Lake City, UT, United States.

It was well documented that both the size of the dendritic field and receptive field of retinal ganglion cells (RGCs) are developmentally regulated in the mammalian retina, and visual stimulation is required for the maturation of the dendritic and receptive fields of mouse RGCs. However, it is not clear whether the developmental changes of the RGC receptive field correlate with the dendritic field and whether visual stimulation regulates the maturation of the dendritic field and receptive field of RGCs in a correlated manner. The present work demonstrated that both the dendritic and receptive fields of RGCs continuously develop after eye opening. However, the correlation between the developmental changes in the receptive field size and the dendritic field varies among different RGC types. These results suggest a continuous change of synaptic converging of RGC synaptic inputs in an RGC type-dependent manner. Besides, light deprivation impairs both the development of dendritic and receptive fields.
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http://dx.doi.org/10.3389/fncel.2021.640421DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8111083PMC
April 2021

Surface-enhanced Raman scattering for mixing state characterization of individual fine particles during a haze episode in Beijing, China.

J Environ Sci (China) 2021 Jun 22;104:216-224. Epub 2020 Dec 22.

MOE Key Laboratory of Resources and Environmental System Optimization, College of Environmental Science and Engineering, North China Electric Power University, Beijing 102206, China; Guangdong Provincial Key Laboratory of Petrochemcial Pollution Processes and Control, School of Environmental Science and Engineering, Guangdong University of Petrochemical Technology, Maoming 525000, China.

The nondestructive characterization of the mixing state of individual fine particles using the traditional single particle analysis technique remains a challenge. In this study, fine particles were collected during haze events under different pollution levels from September 5 to 11 2017 in Beijing, China. A nondestructive surface-enhanced Raman scattering (SERS) technique was employed to investigate the morphology, chemical composition, and mixing state of the multiple components in the individual fine particles. Optical image and SERS spectral analysis results show that soot existing in the form of opaque material was predominant during clear periods (PM ≤ 75 µg/m). During polluted periods (PM > 75 µg/m), opaque particles mixed with transparent particles (nitrates and sulfates) were generally observed. Direct classical least squares analysis further identified the relative abundances of the three major components of the single particles: soot (69.18%), nitrates (28.71%), and sulfates (2.11%). A negative correlation was observed between the abundance of soot and the mass concentration of PM. Furthermore, mapping analysis revealed that on hazy days, PM existed as a core-shell structure with soot surrounded by nitrates and sulfates. This mixing state analysis method for individual PM particles provides information regarding chemical composition and haze formation mechanisms, and has the potential to facilitate the formulation of haze prevention and control policies.
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http://dx.doi.org/10.1016/j.jes.2020.12.008DOI Listing
June 2021

Interactions of functional microorganisms and their contributions to methane bioconversion to short-chain fatty acids.

Water Res 2021 Apr 26;199:117184. Epub 2021 Apr 26.

Advanced Water Management Centre, The University of Queensland, St Lucia, Brisbane, Queensland 4072, Australia. Electronic address:

Methane bioconversion to value-added liquid chemicals has been proposed as a promising solution to augment the petroleum-dominated chemical market. Recent investigations have reported that various electron acceptors (e.g., nitrite and nitrate) are available to drive methane bioconversion to short-chain fatty acids (SCFAs). However, little is known about effects of the rate electron acceptor supplied on liquid chemical production from methane. Herein, three independent membrane biofilm reactors (MBfRs) feeding with respective nitrate, nitrite, combined nitrate and nitrite were operated under high and low rate condition in succession, to study whether feeding rate of electron acceptors could impact the methane bioconversion to SCFAs and the associated microbiological features. Long-term operation showed that all tested electron acceptors with a high supply rate were favorable for methane bioconversion to SCFAs (990.9 mg Ld, 1695.7 mg Ld, and 2425.7 mg Ld), while under a low electron acceptor feeding rate, the SCFA production rate decreased to 8.9 mg Ld, 16.8 mg Ld, and 260.1 mg Ld, respectively. Microbial community characterization showed that the biofilm was predominated by Methanosarcina, Methanobacterium, Propionispora and Clostridium. On the basis of the known metabolism characteristics of these microorganisms, it was assumed that these methanogens and fermenters contributed jointly to methane bioconversion to SCFAs. The findings could be helpful to understand the role of electron acceptor rate in methane bioconversion to liquid chemicals.
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http://dx.doi.org/10.1016/j.watres.2021.117184DOI Listing
April 2021

Acoustic Droplet-Assisted Superhydrophilic-Superhydrophobic Microarray Platform for High-Throughput Screening of Patient-Derived Tumor Spheroids.

ACS Appl Mater Interfaces 2021 May 13. Epub 2021 May 13.

Key Laboratory of Artificial Micro/Nano-Structures, Ministry of Education School of Physics and Technology, Wuhan University, Wuhan 430072, China.

Cell-based high-throughput screening is a key step in the current disease-based research, drug development, and precision medicine. However, it is challenging to establish a rapid culture and screening platform for rare cells (patient-derived) due to the obvious differences between the traditional 2D cell model and the tumor microenvironment, as well as the lack of a low-consumption screening platform for low numbers of cells. Here, we developed an acoustic drop-assisted superhydrophilic-superhydrophobic microarray platform for the rapid culture and screening of a few cells. By employing hydrophilic and hydrophobic microarrays, we can automatically distribute the cell suspension into uniform droplets, and these cells can spontaneously form compact 3D cell spheroids within 36 h (similar to the microenvironment of tumors in vivo). By using the acoustic droplet ejection device, we can accurately inject a drug solution with a volume of ∼pL to ∼nL into the droplet, and the whole process can be completed within 20 ms (one print). By using three different cell lines (Caco-2, MCF-7, and HeLa) to optimize the platform, the culture and screening of five patients' colon cancer were subsequently realized. Using three conventional chemotherapeutics (5-fluorouracil, cetuximab, and panitumumab) of various concentrations, the best treatment was screened out and compared with the actual treatment effect of the patients, and the results were extremely similar. As a proof-of-concept application, we have proved that our platform can quickly cultivate patient samples and effectively screen the best treatment methods, highlighting its wide application in precision medicine, basic tumor research, and drug development.
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http://dx.doi.org/10.1021/acsami.1c06655DOI Listing
May 2021

Soybean residue based biochar prepared by ball milling assisted alkali activation to activate peroxydisulfate for the degradation of tetracycline.

J Colloid Interface Sci 2021 Apr 19;599:631-641. Epub 2021 Apr 19.

College of Environmental Science and Engineering, Hunan University, Lushan South Road, Yuelu District, Changsha 410082, PR China; Key Laboratory of Environmental Biology and Pollution Control (Hunan University), Ministry of Education, Lushan South Road, Yuelu District, Changsha 410082, PR China. Electronic address:

The advanced oxidation process (AOPs) has caused great concern in recent years. Among them, biochar has been widely studied as a catalyst for advanced oxidation process because of its low price and low environmental risk. In this study, a novel ball milling assisted KOH activation biochar (MKBC) was prepared and applied in peroxydisulfate (PDS) activation to degrade tetracycline hydrochloride (TC-H). In comparison with the oxidation (3.48%) by PDS alone and adsorption (36.19%) by MKBC alone, the removal rate of TC-H was increased to 84.15% in the MKBC/PDS system, indicating that MKBC can successfully activate PDS. Besides, the catalytic activity of the MKBC to activate PDS for the degradation of TC-H is 58.33% higher than that of pristine biochar (PBC). In addition, MKBC has outstanding stability that after three repeated experiments, the removal rate of TC-H by the MKBC/PDS system still remains 77.35%. Meanwhile, the mechanism was investigated that the singlet oxygen (O) seized the principal position in the degradation of TC-H in the PDS/MKBC system. This study explored a novel, solvent-free and economic method to propose this extraordinary biochar, which provided a new strategy for the future research of biochar.
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http://dx.doi.org/10.1016/j.jcis.2021.04.074DOI Listing
April 2021

Selenium nanoparticles ameliorate Brassica napus L. cadmium toxicity by inhibiting the respiratory burst and scavenging reactive oxygen species.

J Hazard Mater 2021 Apr 16;417:125900. Epub 2021 Apr 16.

Shandong Key Laboratory of Water Pollution Control and Resource Reuse, School of Environmental Science and Engineering, Shandong University, Qingdao 266237, China. Electronic address:

Cadmium (Cd) is a widely distributed soil contaminant which induces oxidative damage and is therefore toxic to plants. Although selenium oxyanions such as selenite (SeO) and selenate (SeO) can alleviate Cd stress to plants, it is not known whether selenium nanoparticles (SeNPs) are able to do the same. The present study demonstrated the positive impact of both SeNPs and SeO on Brassica napus L. growth under conditions of Cd stress. Underlying mechanisms were elucidated using an oxidative stress detection assay, whole-genome RNA sequencing, and RT-qPCR. Application of selenium, especially in the form of SeNPs, decreased Cd-induced reactive oxygen species production by inhibiting the expression of NADPH oxidases (BnaRBOHC, BnaRBOHD1, and BnaRBOHF1) and glycolate oxidase (BnaGLO), thereby decreasing oxidative protein and membrane lipid damage. In addition, SeNPs improved resistance to Cd stress by decreasing Cd accumulation, maintaining intracellular calcium homeostasis, promoting disulfide bond formation, and restoring the waxy outer layer of the leaf surface. Although both forms of selenium decreased Cd toxicity, the beneficial concentration range was more extensive for SeNPs than for SeO.
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http://dx.doi.org/10.1016/j.jhazmat.2021.125900DOI Listing
April 2021

Bifunctional Noble-Metal-Free Catalyst for the Selective Aerobic Oxidation-Knoevenagel One-Pot Reaction: Encapsulation of Polyoxometalates into an Alkylamine-Modified MIL-101 Framework.

ACS Appl Mater Interfaces 2021 May 11. Epub 2021 May 11.

COMOC-Center for Ordered Materials, Organometallics and Catalysis, Department of Chemistry, Ghent University, Krijgslaan 281, building S3, 9000 Ghent, Belgium.

One-pot reactions offer economic and environmental advantages. Therefore, the design and synthesis of multifunctional catalysts capable of catalyzing multistep organic transformations are highly important. Herein, an effective bifunctional heterogeneous catalyst is presented. For the first time, the encapsulation of HPMoVO (PMoV) polyoxometalate into the cages of an alkylamine-modified MIL-101 using an optimized double-solvent method is reported. The obtained PMoV@DETA-MIL-101 material displays a great catalytic performance (99% conversion of alcohols) for the selective aerobic oxidation-Knoevenagel one-pot reaction. To the best of our knowledge, this is one of the first reports on the usage of noble-metal-free catalysts for the aerobic oxidation-Knoevenagel one-pot reaction without the addition of additives. The catalyst is very stable and can be used for at least five cycles with no leaching of the active sites.
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http://dx.doi.org/10.1021/acsami.1c01621DOI Listing
May 2021

Diagnostic value of endometrial volume and flow parameters under 3D ultrasound acquisition in combination with serum CA125 in endometrial lesions.

Taiwan J Obstet Gynecol 2021 May;60(3):492-497

Department of Obstetrics and Gynecology, The Second Affiliated Hospital of Zhengzhou University, Zhengzhou 450014, China.

Objective: This study aims to discuss the differential diagnosis value of endometrial volume and flow parameters in combination with serum carbohydrate antigen 125 (CA125) in endometrial benign and malignant lesions.

Materials And Methods: The data of 250 patients with endometrial lesions were retrospectively analyzed. Carbohydrate antigen 125 (CA125) was determined before the operation. The morphology, hemodynamics, volume and flow parameters of the endometrium were measured by transvaginal three-dimensional-power Doppler angiography (3D-PDA). The endometrial volume (EV), 3D-PDA vascular index (VI), flow index (FI) and vascularization flow index (VFI) were calculated using the virtual organ computer-aided analysis software (VOCAL).

Results: According to the pathological results, 202 patients (80.8%) had benign endometrial lesions and 48 patients (19.2%) had endometrial cancer (EC). The endometrium of EC patients was thicker (15.64 ± 7.26 mm vs. 9.24 ± 5.06 mm, P < 0.001), the endometrial volume was larger (9.23 ± 4.08 ml vs. 2.26 ± 3.42 ml, P < 0.001), and the flow parameters VI, FI and VFI were higher, when compared to those of benign lesions (P < 0.001). The area under the receiver operating characteristic curve (AUROCC) of VI receptors was 0.86, while the AUC of endometrial thickness (ET) was only 0.66. Therefore, the best variable for distinguishing benign and malignant endometrial lesions was VI. The level of CA125 in the EC group significantly increased (40.57 ± 17.45 vs. 17.87 ± 7.64, P < 0.001), and the level of CA125 increased (P < 0.05) with the increase in clinical grade, degree of tumor differentiation, and pelvic lymph node metastasis (P < 0.05). However, the difference in myometrial invasion was not statistically significant (P > 0.05).

Conclusion: Transvaginal 3D-PDA can clearly show the morphological and hemodynamic characteristics of endometrial lesions, and assist in the detection of EC in combination with serum CA125. This may have important clinical application value.
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http://dx.doi.org/10.1016/j.tjog.2021.03.018DOI Listing
May 2021

Characteristics and Pregnancy Outcomes of Asymptomatic and Symptomatic Women with COVID-19: Lessons from Hospitals in Wuhan.

J Infect Dev Ctries 2021 04 30;15(4):463-469. Epub 2021 Apr 30.

Department of Obstetrics and Gynecology, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China.

Introduction: The objective of the study was to compare the clinical characteristics and pregnancy outcomes of asymptomatic and symptomatic pregnant women with confirmed COVID-19 in the third trimester.

Methodology: Forty-one patients were enrolled in this study from two COVID-19 designated hospitals in Wuhan. Patients underwent chest CT scans for screening and were divided into two groups based on pneumonia-related syndromes. The clinical characteristics and pregnancy outcomes were reviewed and compared.

Results: Among the sample of pregnant women infected with SARS-CoV-2 in the third trimester, there was no mortality or severe complications in the mothers nor newborns. Nearly 40% of the patients in the study were asymptomatic. The most common pneumonia-related symptom in symptomatic pregnant patients was cough. Asymptomatic patients had a significantly shorter duration of hospitalisation and a lower rate of positive RT-PCR testing compared with symptomatic patients. There was no statistically significant difference in antibody test results between asymptomatic and symptomatic patients during hospitalisation, while the positive rate of IgM antibody testing was significantly lower in asymptomatic patients during follow-up.

Conclusions: Clinical manifestation of pregnant women infected with SARS-CoV-2 were atypical and concealed. Screening of possible COVID-19 patients should be strengthened, through serial or combined testing of laboratory testing or radiological testing, before pregnant women are admitted to hospital.
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http://dx.doi.org/10.3855/jidc.14010DOI Listing
April 2021

Global, regional and national burden of anxiety disorders from 1990 to 2019: results from the Global Burden of Disease Study 2019.

Epidemiol Psychiatr Sci 2021 May 6;30:e36. Epub 2021 May 6.

Clinical Epidemiology Unit, Qilu Hospital of Shandong University, Jinan, China.

Aims: Anxiety disorders are widespread across the world. A systematic understanding of the disease burden, temporal trend and risk factors of anxiety disorders provides the essential foundation for targeted public policies on mental health at the national, regional, and global levels.

Methods: The estimation of anxiety disorders in the Global Burden of Disease Study 2019 using systematic review was conducted to describe incidence, prevalence and disability-adjusted life years (DALYs) in 204 countries and regions from 1990 to 2019. We calculated the estimated annual percentage change (EAPC) to quantify the temporal trends in anxiety disorders burden by sex, region and age over the past 30 years and analysed the impact of epidemiological and demographic changes on anxiety disorders.

Results: Globally, 45.82 [95% uncertainty interval (UI): 37.14, 55.62] million incident cases of anxiety disorders, 301.39 million (95% UI: 252.63, 356.00) prevalent cases and 28.68 (95% UI: 19.86, 39.32) million DALYs were estimated in 2019. Although the overall age-standardised burden rate of anxiety disorders remained stable over the past three decades, the latest absolute number of anxiety disorders increased by 50% from 1990. We observed huge disparities in both age-standardised burden rate and changing trend of anxiety disorders in sex, country and age. In 2019, 7.07% of the global DALYs due to anxiety disorders were attributable to bullying victimisation, mainly among the population aged 5-39 years, and the proportion increased in almost all countries and territories compared with 1990.

Conclusion: Anxiety disorder is still the most common mental illness in the world and has a striking impact on the global burden of disease. Controlling potential risk factors, such as bullying, establishing effective mental health knowledge dissemination and diversifying intervention strategies adapted to specific characteristics will reduce the burden of anxiety disorders.
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http://dx.doi.org/10.1017/S2045796021000275DOI Listing
May 2021

Revealing the Superiority of Fast Ion Conductor in Composite Electrolyte for Dendrite-Free Lithium-Metal Batteries.

ACS Appl Mater Interfaces 2021 May 4. Epub 2021 May 4.

School of Chemistry and Materials Science, Hunan Agricultural University, Changsha, Hunan 410128, China.

Composite electrolytes composed of a nanoceramic and polymer have been widely studied because of their high ionic conductivity, good Li-ion transference number, and excellent machinability, whereas the intrinsic reason for the improvement of performance is ambiguous. Herein, we have designed a functional polymer skeleton with different types of nanofiller to reveal the superiority of fast ion conductors in composite electrolyte. Three types of ceramics with different dielectric constants and Li-ion transfer ability were selected to prepare composite electrolytes, the composition, structure, and electrochemical performances of which were systematically investigated. It was found that the addition of fast ion conductive ceramics could provide a high Li-ion transference ability and decreased diffusion barrier because the additional pathways existed in the ceramic, which are revealed by experiment and density functional theory calculations. Benefiting from the superiority of fast ion conductor, Li-metal batteries with this advanced composite electrolyte exhibit an impressive cycling stability and enable a dendrite-free Li surface after cycling. Our work enriches the understanding of the function of fast ion conductors in composite electrolyte and guides the design for other high-performance composite electrolytes in rechargeable solid batteries.
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http://dx.doi.org/10.1021/acsami.1c04115DOI Listing
May 2021

Optimal Timing of Endoscopic Intervention After Extracorporeal Shock-Wave Lithotripsy in the Treatment of Chronic Calcified Pancreatitis.

Pancreas 2021 Apr;50(4):633-638

Department of Gastroenterology, the Second People's Hospital of Karamay, Karamay, Xinjiang Uygur Autonomous Region, China.

Objectives: The interval between extracorporeal shock-wave lithotripsy (ESWL) and endoscopic retrograde cholangiopancreatography (ERCP) may cause differences in cannulation and stone removal. This study was to investigate the optimal timing of ERCP after ESWL.

Methods: Patients with chronic calcified pancreatitis, who underwent ESWL and subsequent ERCP in Changhai Hospital from February 2012 to February 2015, were retrospectively analyzed. The interval between ESWL and ERCP was used to divide patients into groups A (<12 hours), B (12-36 hours), and C (>36 hours). Cannulation success, stone clearance, and post-ESWL/ERCP complications were compared.

Results: A total of 507 patients were enrolled. There were no significant differences regarding the successful cannulation and stone removal rates between the 3 groups. In patients without prior ERCP, the successful cannulation rates were 71.4%, 81.9%, and 90.9% (P = 0.004), and the successful clearance rates were 76.2%, 85.1%, and 90.9% (P = 0.031) for these 3 groups, respectively, showing significant differences. There were no differences in the successful cannulation and stone extraction rates for patients with prior ERCP.

Conclusions: The interval between ESWL and ERCP in chronic calcified pancreatitis patients with prior ERCP is not relevant, while delaying endoscopic intervention is recommended in those with native papilla.
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http://dx.doi.org/10.1097/MPA.0000000000001810DOI Listing
April 2021

Comprehensive Metabolomics Identified the Prominent Role of Glycerophospholipid Metabolism in Coronary Artery Disease Progression.

Front Mol Biosci 2021 14;8:632950. Epub 2021 Apr 14.

Guangdong Provincial People's Hospital, Guangdong Academy of Medical Sciences, School of Medicine, South China University of Technology, Guangzhou, China.

Coronary stenosis severity determines ischemic symptoms and adverse outcomes. The metabolomic analysis of human fluids can provide an insight into the pathogenesis of complex disease. Thus, this study aims to investigate the metabolomic and lipidomic biomarkers of coronary artery disease (CAD) severity and to develop diagnostic models for distinguishing individuals at an increased risk of atherosclerotic burden and plaque instability. Widely targeted metabolomic and lipidomic analyses of plasma in 1,435 CAD patients from three independent centers were performed. These patients were classified as stable coronary artery disease (SCAD), unstable angina (UA), and myocardial infarction (MI). Associations between CAD stages and metabolic conditions were assessed by multivariable-adjusted logistic regression. Furthermore, the least absolute shrinkage and selection operator logistic-based classifiers were used to identify biomarkers and to develop prediagnostic models for discriminating the diverse CAD stages. On the basis of weighted correlation network analysis, 10 co-clustering metabolite modules significantly ( < 0.05) changed at different CAD stages and showed apparent correlation with CAD severity indicators. Moreover, cross-comparisons within CAD patients characterized that a total of 72 and 88 metabolites/lipid species significantly associated with UA (vs. SCAD) and MI (vs. UA), respectively. The disturbed pathways included glycerophospholipid metabolism, and cysteine and methionine metabolism. Furthermore, models incorporating metabolic and lipidomic profiles with traditional risk factors were constructed. The combined model that incorporated 11 metabolites/lipid species and four traditional risk factors represented better discrimination of UA and MI (C-statistic = 0.823, 95% CI, 0.783-0.863) compared with the model involving risk factors alone (C-statistic = 0.758, 95% CI, 0.712-0.810). The combined model was successfully used in discriminating UA and MI patients ( < 0.001) in a three-center validation cohort. Differences in metabolic profiles of diverse CAD subtypes provided a new approach for the risk stratification of unstable plaque and the pathogenesis decipherment of CAD progression.
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http://dx.doi.org/10.3389/fmolb.2021.632950DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8080796PMC
April 2021

The Pharmacological Activity of the Wenjing Decoction in Recurrent Spontaneous Abortion.

Evid Based Complement Alternat Med 2021 13;2021:8861394. Epub 2021 Apr 13.

Department of Traditional Chinese Medicine, Sun Yat-sen Memorial Hospital, SunYat-sen University, Guangzhou 510120, Guangdong, China.

Background: Recurrent spontaneous abortion (RSA) is intractable infertility and can be ameliorated with the use of traditional Chinese medicine preparation, the Wenjing decoction. This study aimed to identify the therapeutic mechanism of Wenjing decoction on specific target proteins involved in RSA.

Methods: Wenjing decoction contains Wuzhuyu, Danggui, Chuanxiong, Guizhi, Shengjiang, Banxia, Gancao, Ejiao, Mudanpi, Chishao, Dangshen, and Maidong. Using TCMSP and BATMAN databases, we queried for active ingredients and predicted their target proteins by BATMAN. Using the edgeR package, we analyzed the differentially expressed genes (DEGs) in the GSE121950 database between control samples and RSA ( = 3). The interaction between DEGs and the predicted target proteins was identified by the Venn diagram. Using the Cytoscape software and clusterProfiler package, enrichment analysis was conducted for the intersected target proteins. Additionally, the protein-protein interaction (PPI) network and pharmacological network were generated using the Cytoscape software.

Results: In total, 31, 2, 7, 7, 5, 13, 93, 11, 29, and 21 active ingredients were identified from Wuzhuyu, Danggui, Chuanxiong, Guizhi, Shengjiang, Banxia, Gancao, Mudanpi, Chishao, and Dangshen, respectively. Additionally, 100 intersected target proteins were revealed by the Venn diagram. Moreover, 98 functional terms and 24 pathways (including C-type lectin receptor signaling pathway, chemokine signaling pathway, leukocyte transendothelial migration, fluid shear stress, and atherosclerosis, and AGE-RAGE signaling pathway in diabetic complications) were enriched. In the PPI network, 10 proteins involved in these five pathways were identified, namely, TNF- (tumor necrosis factor-), IL-10 (interleukin-10), TLR4 (Toll-like receptor 4), JUN (Jun proto-oncogene), IL-1B (interleukin-1-beta), CYBB (cytochrome b558 heavy chain gene), PTGS2 (prostaglandin-endoperoxide synthase 2), APOE (apolipoprotein E), SPI1 (salmonella pathogenicity island 1), and MPO (myeloperoxidase) which showed higher degrees.

Conclusion: The abovementioned genes and pathways might be involved in the pharmacological activity of Wenjing decoction in RSA.
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http://dx.doi.org/10.1155/2021/8861394DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8060116PMC
April 2021

Influence of Gene Polymorphism on Rivaroxaban Blood Concentration and Hemorrhagic Events in Patients With Atrial Fibrillation.

Front Pharmacol 2021 14;12:639854. Epub 2021 Apr 14.

Department of Cardiovascular Medicine, Beijing Hospital, National Center of Gerontology, Institute of Geriatric Medicine, Chinese Academy of Medical Sciences, Beijing, China.

Genetic data on the pharmacokinetics of rivaroxaban and identification of factors that affect its biotransformation, distribution, and excretion will allow for generation of algorithms for personalized use of this drug in patients with atrial fibrillation (AF). Here we tested the effects of (ATP-binding cassette subfamily B member 1) polymorphisms on the valley rivaroxaban blood concentration and on the frequency of hemorrhagic events in patients with AF and propose a personal anticoagulation therapy management protocol. This is a retrospective study. We enrolled Mongolian descent patients who met the criteria from May 2018 to August 2019 in Beijing and Fujian. Clinical data on gender, height, weight, liver and kidney functions, drug trough concentration, and drug dosage were collected; we recorded the bleeding events until 6 months after initiating the medication. single nucleotide polymorphisms including rs1128503, rs1045642, and rs4148738 were identified. After reaching the steady state of plasma concentration, the peripheral blood was collected to detect the trough rivaroxaban plasma concentrations before the next medication. We included 155 patients in this study including 81 men and 74 women, with an average age of 71.98 ± 10.72 years. The distribution of genotypes conformed to the Hardy-Weinberg equilibrium. Multiple comparisons between wild (TT) and mutant (CT and CC) genotypes at the rs1045642 locus showed no significant differences of rivaroxaban trough concentrations (TT vs. CT, = 0.586; TT vs. CC, = 0.802; and CT vs. CC, = 0.702). Multiple comparison between wild (TT) and mutant (CC) genotypes at the rs1128503 locus revealed a significant difference of rivaroxaban trough concentrations (TT vs. CC, = 0.0421). But wild (TT) vs mutant (CT) genotypes and mutant CT vs mutant CC genotypes at the rs1128503 locus showed no significant differences of rivaroxaban trough concentrations (TT vs. CT, = 0.0651; TT vs. CT, = 0.6127). Multiple comparisons between wild (GG) and mutant (AG and AA) genotypes at the rs4148738 locus showed no significant differences of rivaroxaban trough concentrations (GG vs. AG, = 0.341; GG vs. AA, = 0.612; AG vs. AA, = 0.649). There was no significant correlation between ABCB1 gene variation loci rs1045642, rs1128503, rs4148738 and bleeding events. rs1128503 locus variations are correlated with the serum concentration of rivaroxaban in patients of Mongolian descent. But no significant correlation between rs1128503 locus variations and bleeding events were obtained.
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http://dx.doi.org/10.3389/fphar.2021.639854DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8079976PMC
April 2021

Iron overload in the motor cortex induces neuronal ferroptosis following spinal cord injury.

Redox Biol 2021 Apr 22;43:101984. Epub 2021 Apr 22.

Department of Rehabilitation, Southwest Hospital, Third Military Medical University (Army Medical University), Chongqing, China. Electronic address:

Motor neuron death is supposed to result in primary motor cortex atrophy after spinal cord injury (SCI), which is relevant to poorer motor recovery for patients with SCI. However, the exact mechanisms of motor neuron death remain elusive. Here, we demonstrated that iron deposition in the motor cortex was significantly increased in both SCI patients and rats, which triggered the accumulation of lipid reactive oxygen species (ROS) and resulted in motor neuronal ferroptosis ultimately. While iron chelator, ROS inhibitor and ferroptosis inhibitor reduced iron overload-induced motor neuron death and promoted motor functional recovery. Further, we found that activated microglia in the motor cortex following SCI secreted abundant nitric oxide (NO), which regulated cellular iron homeostasis-related proteins to induce iron overload in motor neurons. Thus, we conclude that microglial activation induced iron overload in the motor cortex after SCI triggered motor neuronal ferroptosis and impeded motor functional recovery. These findings might provide novel therapeutic strategies for SCI.
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http://dx.doi.org/10.1016/j.redox.2021.101984DOI Listing
April 2021

Pharmacological targeting of NLRP3 deubiquitination for treatment of NLRP3-associated inflammatory diseases.

Sci Immunol 2021 Apr;6(58)

State Key Laboratory of Proteomics, Beijing Proteome Research Center, National Center for Protein Sciences (Beijing), Beijing Institute of Lifeomics, Beijing 102206, China.

Pharmacologically inhibiting nucleotide-binding domain and leucine-rich repeat-containing (NLR) family, pyrin domain-containing protein 3 (NLRP3) inflammasome activation results in potent therapeutic effects in a wide variety of preclinical inflammatory disease models. NLRP3 deubiquitination is essential for efficient NLRP3 inflammasome activity, but it remains unclear whether this process can be harnessed for therapeutic benefit. Here, we show that thiolutin (THL), an inhibitor of the JAB1/MPN/Mov34 (JAMM) domain-containing metalloprotease, blocks NLRP3 inflammasome activation by canonical, noncanonical, alternative, and transcription-independent pathways at nanomolar concentrations. In addition, THL potently inhibited the activation of multiple NLRP3 mutants linked with cryopyrin-associated periodic syndromes (CAPS). Treatment with THL alleviated NLRP3-related diseases in mouse models of lipopolysaccharide-induced sepsis, monosodium urate-induced peritonitis, experimental autoimmune encephalomyelitis, CAPS, and methionine-choline-deficient diet-induced nonalcoholic fatty liver disease. Mechanistic studies revealed that THL inhibits the BRCC3-containing isopeptidase complex (BRISC)-mediated NLRP3 deubiquitination and activation. In addition, we show that holomycin, a natural methyl derivative of THL, displays an even higher inhibitory activity against NLRP3 inflammasome than THL. Our study validates that posttranslational modification of NLRP3 can be pharmacologically targeted to prevent or treat NLRP3-associated inflammatory diseases. Future clinical development of derivatives of THL may provide new therapies for NLRP3-related diseases.
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http://dx.doi.org/10.1126/sciimmunol.abe2933DOI Listing
April 2021

Bioassay-guided isolation and characterization of antibacterial compound from HX-1 associated with Clam.

3 Biotech 2021 Apr 25;11(4):193. Epub 2021 Mar 25.

Jiangsu Key Laboratory of Marine Bioresources and Environment, Co-Innovation Center of Jiangsu Marine Bio-Industry Technology, Jiangsu Ocean University, Lianyungang, 222005 China.

This study aimed to identify a symbiotic fungus strain HX-1 with anti- activity and isolate and identify the active compound. The HX-1 strain was identified as according to the morphological characteristics and internal transcribed spacer (ITS) sequence analysis. The compound was isolated from the fermentation product of HX-1 strain through ethyl acetate extraction, silica gel and Sephadex LH-20 column chromatography, and semi-preparative HPLC techniques using an antibacterial-guided fractionation method. According to its physicochemical properties and spectral characteristics, the compound was identified as trypacidin having the same anti- activity as streptomycin sulfate, with the minimum inhibitory concentration of 31.25 µg/mL.
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http://dx.doi.org/10.1007/s13205-021-02754-3DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7994498PMC
April 2021

Risk factors for femoral neck fracture in elderly population.

Zhong Nan Da Xue Xue Bao Yi Xue Ban 2021 Mar;46(3):272-277

Department of Orthopedics, Northern Jiangsu People's Hospital, Yangzhou Jiangsu 225001, China.

Objectives: To explore the risk factors for femoral neck fracture in elderly population.

Methods: A total of 124 elderly patients (≥60 years old) in hospital for trauma were enrolled, including 71 patients (57%) with femoral neck fracture and 53 non-femoral neck fracture patients (43%). All patients' age, gender, body mass index (BMI), bone mineral density (BMD), thigh length and average circumference were collected. Single factor analysis and multivariate logistic regression analysis were performed to explore whether the above factors were risk factors for femoral neck fracture.

Results: Single factor analysis showed that the age, gender, BMI, BMD, thigh length, and average thigh circumference between the 2 groups were statistically different (all <0.05), and multi-factor logistic regression analysis showed that age, gender, BMI, BMD, thigh length, and average thigh circumference were influencing factors for femoral neck fracture in elderly population (all <0.05).

Conclusions: Older age, female, lower BMI index (low body weight), lower BMD (osteoporosis), longer thigh length, and lower average circumference are risk factors for femoral neck fracture in the elderly population.
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http://dx.doi.org/10.11817/j.issn.1672-7347.2021.190378DOI Listing
March 2021

Dynamic Compressive Strength Tests of Corroded SFRC Exposed to Drying-Wetting Cycles with a 37 mm Diameter SHPB.

Materials (Basel) 2021 Apr 27;14(9). Epub 2021 Apr 27.

College of Architecture and Civil Engineering, Wenzhou University, Wenzhou 325035, China.

This study focuses on the dynamic compression performance of corroded steel fiber-reinforced concrete (SFRC) exposed to drying-wetting chloride cycles by a 37 mm diameter split Hopkinson pressure bar (SHPB) system. Three steel fiber contents (0.5%, 1.0%, 2.0%, by volume) were incorporated into concrete, and samples were subjected to drying-wetting cycles for different corrosion durations (30 days, 60 days, 90 days) after 28 days age. The sample damage mode, stress-strain curve and the dynamic compression performance of corroded SFRC were compared with plain concrete. Through the experimental data, strain-rate effect, fiber reinforcement effect and the corrosion duration influence on the impact compression property of SFRC were identified. The dynamic increase factor results of these samples were compared with the existing models in previous published literature. An empirical dynamic increase factor profile characterization model considering fiber content, corrosion duration and strain-rate is proposed.
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http://dx.doi.org/10.3390/ma14092267DOI Listing
April 2021

Incidence of Gibelio Gill Hemorrhagic Disease Caused by CyHV-2 Infection Can Be Reduced by Vaccination with Polyhedra Incorporating Antigens.

Vaccines (Basel) 2021 Apr 16;9(4). Epub 2021 Apr 16.

School of Biology and Basic Medical Sciences, Soochow University, Suzhou 215123, China.

Encapsulation of antigens within protein microcrystals (polyhedra) is a promising approach for the stable delivery of vaccines. In this study, a vaccine was encapsulated into polyhedra against cyprinid herpesvirus II (CyHV-2). CyHV-2 typically infects gibel carp, gibelio, causing gill hemorrhagic disease. The vaccine was constructed using a codon-optimized sequence, D4ORF, comprising the ORF72 (region 1-186 nt), ORF66 (region 993-1197 nt), ORF81 (region 603-783 nt), and ORF82 (region 85-186 nt) genes of CyHV-2. The H1-D4ORF and D4ORF-VP3 sequences were, respectively, obtained by fusing the H1-helix sequence (region 1-90 nt) ofBombyx mori cypovirus(BmCPV) polyhedrin to the 5' terminal end of D4ORF and by fusing a partial sequence (1-279 nt) of the BmCPV VP3 gene to the 3' terminal end of D4ORF. Furthermore, BmNPV-H1-D4ORF-polh and BmNPV-D4ORF-VP3-polh recombinant nucleopolyhedroviruses (BmNPVs), belonging to the family Baculoviridae, and co-expressing BmCPV polyhedrin and H1-D4ORF or D4ORF-VP3, were constructed. H1-D4ORF and D4ORF-VP3 fusion proteins were confirmed to be encapsulated into recombinant cytoplasmic polyhedra by Western blotting. Degradation of vaccine proteins was assessed by SDS-PAGE, and the results showed that the encapsulated vaccine proteins in polyhedra could be protected from degradation. Furthermore, when gibel carp were vaccinated with the purified polyhedra from BmNPV-H1-D4ORF-polh and BmNPV-D4ORF-VP3-polh via injection, the antibody titers in the serum of the vaccinated fish reached 1:6400-1:12,800 at 3 weeks post-vaccination. Therelative percentage of survival of immunized gibel carp reached 64.71% and 58.82%, respectively, following challenge with CyHV-2. These results suggest that incorporating vaccine protein into BmCPV polyhedra may be a novel approach for developing aquaculture microencapsulated vaccines.
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http://dx.doi.org/10.3390/vaccines9040397DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8072653PMC
April 2021

Introspective False Negative Prediction for Black-Box Object Detectors in Autonomous Driving.

Sensors (Basel) 2021 Apr 16;21(8). Epub 2021 Apr 16.

School of Automotive Studies, Tongji University, Shanghai 201804, China.

Object detection plays a critical role in autonomous driving, but current state-of-the-art object detectors will inevitably fail in many driving scenes, which is unacceptable for safety-critical automated vehicles. Given the complexity of the real traffic scenarios, it is impractical to guarantee zero detection failure; thus, online failure prediction is of crucial importance to mitigate the risk of traffic accidents. Of all the failure cases, False Negative (FN) objects are most likely to cause catastrophic consequences, but little attention has been paid to the online FN prediction. In this paper, we propose a general introspection framework that can make online prediction of FN objects for black-box object detectors. In contrast to existing methods which rely on empirical assumptions or handcrafted features, we facilitate the FN feature extraction by an introspective FN predictor we designed in this framework. For this purpose, we extend the original concept of introspection to object-wise FN predictions, and propose a multi-branch cooperation mechanism to address the distinct foreground-background imbalance problem of FN objects. The effectiveness of the proposed framework is verified through extensive experiments and analysis, and the results show that our method successfully predicts the FN objects with 81.95% precision for 88.10% recall on the challenging KITTI Benchmark, and effectively improves object detection performance by taking FN predictions into consideration.
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http://dx.doi.org/10.3390/s21082819DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8073889PMC
April 2021

Synthesis and Bioactivities of Marine Pyran-Isoindolone Derivatives as Potential Antithrombotic Agents.

Mar Drugs 2021 Apr 15;19(4). Epub 2021 Apr 15.

College of Food Science and Technology, Shanghai Ocean University, Shanghai 201306, China.

2,5-Bis-[8-(4,8-dimethyl-nona-3,7-dienyl)-5,7-dihydroxy-8-methyl-3-keto-1,2,7,8-teraahydro-6-pyran[]isoindol-2-yl]-pentanoic acid (FGFC1) is a marine pyran-isoindolone derivative isolated from a rare marine microorganism FG216, which showed moderate antithrombotic(fibrinolytic) activity. To further enhance its antithrombotic effect, a series of new FGFC1 derivatives (-) were synthesized via chemical modification at C-2 and C-2' phenol groups moieties and C-1″ carboxyl group. Their fibrinolytic activities in vitro were evaluated. Among the derivatives, - and showed significant fibrinolytic activities with EC of 59.7, 87.1, 66.6, 82.8, and 42.3 μM, respectively, via enhancement of urokinase activity. Notably, derivative presented the most remarkable fibrinolytic activity (2.72-fold than that of FGFC1). Furthermore, the cytotoxicity of derivative was tested as well as expression of Fas/Apo-1 and IL-1 on HeLa cells. The results showed that, compared to FGFC1, derivative possessed moderate cytotoxicity and apoptotic effect on HeLa cells (statistical significance > 0.1), making a potential antithrombotic agent towards clinical application.
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http://dx.doi.org/10.3390/md19040218DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8071544PMC
April 2021

Clinical outcomes, quality of life, and costs evaluation of peritoneal dialysis management models in Shanghai Songjiang District: a multi-center and prospective cohort study.

Ren Fail 2021 Dec;43(1):754-765

Department of Nephrology, Shanghai East Hospital, Tongji University School of Medicine, Shanghai, China.

The new Family-Community-Hospital (FCH) three-level comprehensive management aimed to improve the efficiency and scale of peritoneal dialysis (PD) to meet the increased population of end-stage renal disease (ESRD). Our study focused on the clinical outcomes, quality of life, and costs evaluation of this model in a multi-center and prospective cohort study. A total of 190 ESRD patients who commenced PD at Shanghai Songjiang District were enrolled. According to different PD management models, patients were divided into the Family-Community-Hospital three-level management model ( = 90) and the conventional all-course central hospital management model ( = 100). The primary outcome was clinical outcomes of PD. The secondary outcomes were health-related quality of life (HRQOL) and medical costs evaluation. Compared to conventional management, community-based FCH management achieved a similar dialysis therapeutic effect, including dropout rate ( = 0.366), peritonitis rate ( = 0.965), patient survival ( = 0.441), and technique survival ( = 0.589). Follow-up data showed that similar levels of the renal and peritoneal functions, serum albumin, cholesterol and triglyceride, PTH, serum calcium, and phosphorus between the two groups (all  > 0.05). HRQOL survey showed that the FCH management model helped to improve the psychological status of PD patients, including social functioning ( = 0.006), role-emotional ( = 0.032), and mental health ( = 0.036). FCH management also reduced the hospitalization ( = 0.009) and outpatient visits ( = 0.001) and saved annual hospitalization costs ( = 0.005), outpatient costs ( = 0.026), and transport costs ( = 0.006). Compared with conventional management, community-based FCH management achieved similar outcomes, improved psychological health, reduced medical budgets, and thus had a good social prospect.
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http://dx.doi.org/10.1080/0886022X.2021.1918164DOI Listing
December 2021

Carbon Monoxide Releasing Molecule-3 Enhances Osteogenic Differentiation of Human Periodontal Ligament Stem Cells by Carbon Monoxide Release.

Drug Des Devel Ther 2021 22;15:1691-1704. Epub 2021 Apr 22.

Department of VIP Center, School and Hospital of Stomatology, Cheeloo College of Medicine, Shandong University & Shandong Key Laboratory of Oral Tissue Regeneration & Shandong Engineering Laboratory for Dental Materials and Oral Tissue Regeneration, Jinan, 250012, Shandong, People's Republic of China.

Purpose: Limited intrinsic regeneration capacity following bone destruction remains a significant medical problem. Multiple regulatory effects of carbon monoxide releasing molecule-3 (CORM-3) have been reported. The aim of this study was to investigate the effect of CORM-3 on the osteogenic differentiation of human periodontal ligament stem cells (hPDLSCs) during osteogenesis.

Patients And Methods: hPDLSCs obtained from healthy periodontal ligament tissues were cultured and identified with specific surface antigens by flow cytometry. Effect of CORM-3 on the proliferation of hPDLSCs was determined by CCK-8 assay. Alizarin red staining and alkaline phosphatase (ALP) activity were used to assess the osteogenic differentiation of hPDLSCs. Real-time quantitative polymerase chain reaction (RT-qPCR) and Western blot analysis were used to detect the expression of the indicated genes. Critical-sized skull defect was made in Balb/c-nude mice, microcomputed tomography (Micro-CT) and Masson trichrome staining were used to assess the new bone regeneration in mice.

Results: CORM-3 (400 μmol/l) significantly promoted the proliferation of hPDLSCs. CORM-3 pretreatment not only notably enhanced the mRNA and protein expression of osteo-specific marker OPN, Runx2 and ALP, but also increased mineral deposition and ALP activity by the release of CO on day 3, 7 and 14 (P<0.05). Degassed CORM-3 did not show the same effect as CORM-3. In animal model, application of CORM-3 with hPDLSCs transplantation highly increased new bone formation in skull defect region.

Conclusion: CORM-3 promoted osteogenic differentiation of hPDLSCs, and increased hPDLSCs-induced new bone formation in mice with critical-sized skull defect, which suggests an efficient and promising strategy in the treatment of disease with bone defect.
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http://dx.doi.org/10.2147/DDDT.S300356DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8075314PMC
April 2021

Transcriptomic responses predict the toxic effect of parental co-exposure to dibutyl phthalate and diisobutyl phthalate on the early development of zebrafish offspring.

Aquat Toxicol 2021 Jun 16;235:105838. Epub 2021 Apr 16.

School of the Chemistry and Chemical Engineering, Jiangsu University, Zhenjiang 212013, People's Republic of China.

Dibutyl phthalate (DBP) and diisobutyl phthalate (DiBP) have been reported to exhibit reproductive toxicity in vertebrates. However, the combined effect of DBP and DiBP on offspring of exposed parents remains unclear, especially for aquatic organisms such as fish. The aims of this study were to assess the effects of parental co-exposure to DBP and DiBP on early development of zebrafish offspring, and to explore the potential molecular mechanisms involved. The early developmental indicators and transcriptomic profiles of F1 larvae were examined after parental exposure to DBP, DiBP and their mixtures (Mix) for 30 days. Results showed that parental exposure to DBP and DiBP, alone or in combination, resulted in increased hatchability at 48 hpf and heart rate at 96 hpf, and increased the prevalence of malformations and mortality in F1 larvae. Generalized linear model (GLM) suggested an antagonistic interactive effect between DBP and DiBP on mortality and malformations of F1 larvae. The transcriptomic analysis revealed that the molecular mechanisms of parental co-exposure were different from those of either chemical alone. Disruption of molecular functions involved unfolded protein binding, E-box binding and photoreceptor activity in F1 larvae. These findings provide initial insights in the potential mechanism of action of parental co-exposure to DBP and DiBP.
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http://dx.doi.org/10.1016/j.aquatox.2021.105838DOI Listing
June 2021

Investigating mediated public engagement with science on the "science" subreddit: From the participants' perspective.

PLoS One 2021 28;16(4):e0249181. Epub 2021 Apr 28.

Information & Library Science, Luddy School of Informatics, Computing & Engineering, Indiana University, Bloomington, Indiana, United States of America.

While public engagement with science activities traditionally inhabits physical environments (i.e., museum exhibits), as the Internet becomes more ubiquitous, new types of public engagement with science mediated through information technologies have emerged. Instead of having scientific findings filtered through traditional mediators, scientists have begun to take advantage of social media in order to communicate directly with the general public. This paper focuses on technology mediated public engagement with science in an online environment, specifically the sub-Reddit called "r/science", on a popular platform, Reddit, in which we investigated the factors contributing to user engagement and perceived effects of science communication from the users' perspectives. The survey instrument including user engagement scales, perceived effects of science communication, and demographics were distributed among 2000 participants in the r/science Ask Me Anything (AMA) series. We analyzed 146 survey responses using descriptive statistics and ordinal logistic regression. The findings indicated that the participants were generally engaged compared to ones in other studies that used the same user engagement scales and perceived positive effects on science communication, except when it came to building trust. Furthermore, we found that time spent on this particular platform appeared to be the most important factor when it came to positive perceived effects of r/science AMAs. This type of mediated public engagement has been insufficiently investigated, most particularly in terms of the examination of participants' perspectives. This void is addressed in this study. The findings from the study will also be informative to similar platforms that support mediated public engagement with science.
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http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0249181PLOS
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8081246PMC
April 2021

Fast detection of with a novel fluorescent biosensor based on a FRET system between UCNPs and GO@FeO in urine specimens.

Anal Methods 2021 Apr 27. Epub 2021 Apr 27.

Clinical Laboratories, The First Affiliated Hospital of Chongqing Medical University, Chongqing, 400016, P. R. China.

Biosensors based on nanomaterials are becoming a research hotspot for the rapid detection of pathogenic bacteria. Herein, a "turn-on" fluorescent biosensor based on a FRET system was constructed for the fast detection of a representative pathogenic microorganism, namely, E. coli, which causes most urinary tract infections. This biosensor was constructed by utilizing synthesized UCNPs as fluorescent donors with stable luminescence performance in complex biological samples and GO@Fe3O4 as a receptor with both excellent adsorption ability and fluorescence quenching ability. A specific ssDNA selected as an aptamer which could recognize E. coli was immobilized on the UCNPs to form UCNP-Apt nanoprobes. The nanoprobes were adsorbed on the surface of GO@Fe3O4 through the π-stacking interactions between aptamers and GO. In the presence of E. coli, UCNP-Apt nanoprobes detached from GO@Fe3O4 due to the specific recognition of aptamers and bacteria, resulting in obvious fluorescence recovery, and the concentration of bacteria was positively correlated with the intensity of the fluorescence signal; such a "turn-on" signal output mode ensures excellent precision. In addition, the easy magnetic separation of GO@Fe3O4 simplifies the operation process, helping the sensor detect bacteria in 30 minutes with a linear range from 103 to 107 CFU mL-1 and a limit of detection of 467 CFU mL-1. Moreover, recovery test results also showed that the sensor has clinical application potential for the rapid detection of pathogenic microorganisms in complex biological samples.
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http://dx.doi.org/10.1039/d1ay00320hDOI Listing
April 2021

Association between different peritoneal dialysis catheter placement methods and short-term postoperative complications.

BMC Nephrol 2021 Apr 26;22(1):151. Epub 2021 Apr 26.

Department of Ultrasound, the Third Affiliated Hospital of Soochow University, 213003, Changzhou, China.

Background: Considering that current peritoneal dialysis has its own shortcomings, In this study, the Seldinger technique was modified to explore the relationship between different catheter placement methods of peritoneal dialysis and short-term postoperative complications.

Methods: We retrospectively analyzed the data of 157 patients who received peritoneal dialysis in the Department of Nephrology of our hospital from January 2017 to December 2019. According to different catheter placement methods, the patients were divided into three groups: 111 cases of open surgery technique, 23 cases of Seldinger technique, and 23 cases of modified Seldinger technique (ultrasound-guided Veress needle puncture). The general data, laboratory indexes, and abdominal infection and catheter-related complications within one month postoperatively were collected.

Results: There were 48 (31.0 %) cases of complications in 157 patients within one month postoperatively, which were mainly catheter-related complications (45 cases, 29.0 %). The incidence of catheter tip peritoneal drift (catheter migration) in the three groups was 27.3 %, 39.1 %, and 9.1 %, respectively, with no significant difference between groups (P = 0.069). Univariate logistic regression analysis showed that the systolic blood pressure, history of abdominal and pelvic surgery, creatinine, and modified Seldinger technique were possible impact factors of catheter migration (P < 0.10). After fully adjusting for confounding factors, Compared with the open surgery group, the modified Seldinger method group significantly reduced the risk of catheter migration with an OR of 0.161 (95 % confidence interval: 0.027-0.961, P = 0.045); However, the difference between the Seldinger method group and the open surgery group was not significant, with an OR of 1.061 (95 % confidence interval: 0.308-3.649, P = 0.926). Curve fitting showed that the average incidence of catheter migration in the three groups was 27.3 % (95% CI: 15.9-42.7 %), 28.5 % (95% CI: 10.7-56.9 %), and 5.7 % (95% CI: 1.0-27.0 %); the modified Seldinger method has the lowest average incidence of catheter migration.

Conclusions: Modified Seldinger technique can significantly reduce catheter-related short-term complications after peritoneal dialysis, and it is especially effective in reducing the incidence of catheter migration. Modified Seldinger technique is a safe and feasible method for the placement of a peritoneal dialysis catheter.
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http://dx.doi.org/10.1186/s12882-021-02340-yDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8074448PMC
April 2021