Publications by authors named "Hugo Morin"

5 Publications

  • Page 1 of 1

Identification of Potential Antiseizure Agents in using Zebrafish and Mouse Epilepsy Models.

ACS Chem Neurosci 2021 05 29;12(10):1791-1801. Epub 2021 Apr 29.

School of Pharmaceutical Sciences, University of Geneva, CMU-Rue Michel-Servet 1, CH-1211 Geneva 4, Switzerland.

The resin of the tree Flueck. (synonym: ; Burseraceae), also known as "frankincense", is a traditional remedy used for central nervous system disorders in East Africa. Here we report the evaluation of its antiseizure activity in zebrafish and mouse epilepsy models to identify novel antiseizure compounds. The resin was extracted by solvents of increasing polarity. The hexane extract demonstrated the strongest antiseizure activity and was therefore subjected to bioactivity-guided isolation, which leaded to the isolation of eight terpene derivatives. A new prenylbicyclogermacrene derivative () was isolated along with seven other compounds (, -). Among them, the triterpene β-boswellic acid () showed the strongest activity and reduced 90% of pentylenetetrazole (PTZ)-induced seizures at 100 μg/mL. In parallel to , a commercial extract of was also evaluated and showed moderate bioactivity (45% reduction at 30 μg/mL). The extract of was subjected to targeted isolation of other boswellic acid derivatives (-), which were evaluated for antiseizure activity in comparison with . In the whole series, β-boswellic acid () was the most active (60% reduction at 200 μM), and its potency was also confirmed with its purchased standard (). Pure nanoparticles of and a commercially formulated extract of were tested in a PTZ-kindling mouse seizure model. This notably revealed that the administration reduced seizures by 50% in this mouse model, which was consistent with its detection and quantification in plasma and brain samples. This study and the preclinical evaluation performed indicate that β-boswellic acid, common to various species of , has some potential as an antiseizure agent.
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http://dx.doi.org/10.1021/acschemneuro.1c00044DOI Listing
May 2021

In Vitro Anti-Inflammatory Activity in Arthritic Synoviocytes of Root Extracts and Its Unusual Dimeric Flavonoids.

Molecules 2020 Nov 9;25(21). Epub 2020 Nov 9.

School of Pharmaceutical Sciences, University of Geneva, 1211 Geneva-4, Switzerland.

is a plant commonly used for the treatment of kidney stones, arthritis and pain in traditional Brazilian medicine. Different in vitro and in vivo activities, ranging from antinociceptive to anti-, have been reported for the dichloromethane root extract of (DCMAB) and isolated compounds. This work aimed to assess the in vitro anti-inflammatory activity in arthritic synoviocytes of the DCMAB, the hydroethanolic extract (HEAB) and three dimeric flavonoids isolated from the DCMAB. These compounds, brachydin A (), B () and C (), were isolated both by medium pressure liquid and high-speed counter current chromatography. Their quantification was performed by mass spectrometry on both DCMAB and HEAB. IL-1β activated human fibroblast-like synoviocytes were incubated with both extracts and isolated compounds to determine the levels of pro-inflammatory cytokine IL-6 by enzyme-linked immunosorbent assay (ELISA). DCMAB inhibited 30% of IL-6 release at 25 µg/mL, when compared with controls while HEAB was inactive. IC values determined for and were 3-fold higher than . The DCMAB activity seems to be linked to higher proportions of compounds and in this extract. These observations could thus explain the traditional use of roots in the treatment of osteoarthritis.
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http://dx.doi.org/10.3390/molecules25215219DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7665123PMC
November 2020

Production of Highly Active Antiparasitic Compounds from the Controlled Halogenation of the Crude Plant Extract.

J Nat Prod 2020 09 9;83(9):2631-2640. Epub 2020 Sep 9.

School of Pharmaceutical Sciences and Institute of Pharmaceutical Sciences of Western Switzerland (ISPSW), University of Geneva, CMU, Rue Michel Servet 1, 1211 Geneva 4, Switzerland.

Direct halogenation of phenolic compounds present in the CHCl extract of the roots of was investigated to enhance chemodiversity. The approach is based on eco-friendly reactions using NaBr, NaI, and NaCl in aqueous media to generate multiple "unnatural" halogenated natural products from crude extracts. The halogenation reactions, monitored by UHPLC-PDA-ELSD-MS, were optimized to generate mono-, di-, or trihalogenated derivatives. To isolate these compounds, the reactions were scaled up and the halogenated analogues were isolated by semipreparative HPLC-UV and fully characterized by NMR and HR-MS data. All of the original 16 halogenated derivatives were evaluated for their antiparasitic activities against the parasites and . Compounds presenting selective antiparasitic activities against one or both parasites with IC values comparable to the reference were identified.
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http://dx.doi.org/10.1021/acs.jnatprod.0c00433DOI Listing
September 2020

Impact of storage temperature and processing delays on cord blood quality: discrepancy between functional in vitro and in vivo assays.

Transfusion 2012 Nov 15;52(11):2401-5. Epub 2012 Apr 15.

Department of Pediatrics, CHU Sainte-Justine Research Center, CHU Sainte-Justine, University of Montréal, Montréal, Québec, Canada.

Background: Optimal conditions of cord blood (CB) storage, processing, cryopreservation, and thawing are critical for banking and transplantation. Nevertheless, standardized procedures are still awaited.

Study Design And Methods: We evaluated the impact of preprocessing storage and temperature on recovery, viability, and functional differentiation capacities of hematopoietic progenitor cells. We compared units stored at room temperature (RT) or at 4 °C for 72 hours before cryopreservation to units processed shortly after collection (<12 hr).

Results: Postthaw results showed similar in vitro characteristics between immediate processing and 4 °C storage for cell recovery and viability, both significantly higher than RT storage. Surprisingly, we demonstrated that storage of CB units at RT before processing and cryopreservation profoundly altered in vivo hematopoietic reconstitution in mice, although in vitro hematopoietic colony-forming unit potential was unaltered.

Conclusion: Our findings challenge current CB storage practices and suggest standard in vitro quality assessments may not always be indicative of CB engraftment potential.
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http://dx.doi.org/10.1111/j.1537-2995.2012.03650.xDOI Listing
November 2012

Assessment of cord blood unit characteristics on the day of transplant: comparison with data issued by cord blood banks.

Transfusion 2006 Jul;46(7):1190-8

Division of Hematology-Oncology, CHU Sainte-Justine, Université de Montréal, Montréal, Québec, Canada.

Background: Selection of a cord blood (CB) unit for allogeneic transplantation relies on graft characterization results provided by cord blood banks (CBBs). The goal was to compare the graft characterization results obtained upon thawing and washing to those provided by CBBs at selection.

Study Design And Methods: With tests that assess CB graft characteristics known to impact engraftment, CB units have been analyzed after thaw and before infusion. Our results were compared to data provided by CBBs to determine the impact on engraftment and assess how CBB-supplied information can affect future CB unit selection.

Results: Variability was noted as to the type of information provided by the different CBBs. Also, variability was found between the information provided by CBBs and the graft characterization results obtained upon thawing and washing. In some cases, CB measures known to be predictive of engraftment were found much lower than reported by CBBs. Only the total nucleated cell count, which is the main CB graft selection criterion besides HLA matching, correlated favorably.

Conclusions: Our data reveal a high degree of variability in graft characteristics provided by CBBs and often poor correlation with results obtained on thawed and washed CB units. We suggest that standardized laboratory procedures aimed at graft characterization should be used by both CBBs and transplant centers to avoid unacceptable discrepancies.
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http://dx.doi.org/10.1111/j.1537-2995.2006.00870.xDOI Listing
July 2006
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