Publications by authors named "Huayang Li"

31 Publications

A New Minimally Invasive Method of Transverse Aortic Constriction in Mice.

J Cardiovasc Transl Res 2021 Sep 8. Epub 2021 Sep 8.

Department of Cardiac Surgery, The First Affiliated Hospital of Sun Yat-Sen University, 58 Zhongshan II Road, Guangzhou, 510080, Guangdong Province, China.

Transverse aortic constriction (TAC) in mice is the most popular model to mimic pressure overload heart disease. In this study, we developed a convenient, quick, and less invasive new TAC mice model. Briefly, after anesthetization, endotracheal intubation was then performed, and the endotracheal tube was connected to a ventilator. The second intercostal space was opened and then the home-made retractors were used to push aside the thymus gently. A tunnel under the aortic arch was made and a segment of 6-0 monofilament polypropylene suture which had been threaded through a specifically modified blunted 26-gauge syringe needle was passed through the tunnel. A blunted 27-gauge needle was placed parallel to the transverse aorta and then three knots were tied quickly. After ligation, the spacer was removed promptly and gently to achieve a constriction of 0.4 mm in diameter. Five weeks after TAC, cardiac hypertrophy, fibrosis, and left ventricular dysfunction were observed. The mouse was anesthetized with pentobarbital (50 mg/kg) via intraperitoneal injection. Endotracheal intubation under direct vision was then performed and the endotracheal tube was connected to a ventilator. The second intercostal space was opened and then the home-made retractors were used to push aside the thymus gently. A tunnel under the aortic arch was made and a segment of 6-0 monofilament polypropylene suture which had been threaded through a specifically modified blunted 26-gauge syringe needle was passed through the tunnel.
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http://dx.doi.org/10.1007/s12265-021-10170-4DOI Listing
September 2021

Transcriptional regulation and functional analysis of Nicotiana tabacum under salt and ABA stress.

Biochem Biophys Res Commun 2021 Sep 16;570:110-116. Epub 2021 Jul 16.

Agricultural Big-Data Research Center and College of Plant Protection, Shandong Agricultural University, Taian, 271018, China. Electronic address:

Soil salinization is an important factor that restricts crop quality and yield and causes an enormous toll to human beings. Salt stress and abscisic acid (ABA) stress will occur in the process of soil salinization. In this study, transcriptome sequencing of tobacco leaves under salt and ABA stress in order to further study the resistance mechanism of tobacco. Compared with controlled groups, 1654 and 3306 DEGs were obtained in salt and ABA stress, respectively. The genes function enrichment analysis showed that the up-regulated genes in salt stress were mainly concentrated in transcription factor WRKY family and PAR1 resistance gene family, while the up-regulated genes were mainly concentrated on bHLH transcription factor, Kunitz-type protease inhibitor, dehydrin (Xero1) gene and CAT (Catalase) family protein genes in ABA stress. Tobacco MAPK cascade triggered stress response through up-regulation of gene expression in signal transduction. The expression products of these up-regulated genes can improve the abiotic stress resistance of plants. These results have an important implication for further understanding the mechanism of salinity tolerance in plants.
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http://dx.doi.org/10.1016/j.bbrc.2021.07.011DOI Listing
September 2021

Characterization of Chromatin Accessibility and Gene Expression Upon Cold Stress Reveals the Transcription Factor RAV1 Functions in Cold Response in Vitis amurensis.

Plant Cell Physiol 2021 Jul 19. Epub 2021 Jul 19.

Beijing Key Laboratory of Grape Sciences and Enology, Key Laboratory of Plant Resource, Institute of Botany, Chinese Academy of Sciences, Beijing 100093, PR China.

Cold tolerance is regulated by a variety of transcription factors (TFs) and their target genes. Except for the well-characterized C-repeat binding factors (CBFs)-dependent transcriptional cascade, the mechanisms of cold tolerance mediated by other transcriptional regulatory networks are still largely unknown. Here we used the assay for transposase-accessible chromatin with sequencing (ATAC-seq) and RNA-seq to identify cold responsive TFs in Vitis amurensis, a grape species with high cold hardiness. A number of 9 TFs, including CBF4, RAV1 and ERF104, were identified after cold treatment. Weighted gene co-expression network analysis (WGCNA) and gene ontology (GO) analysis revealed that these TFs may regulate cold response through different pathways. As a prime candidate TF, overexpression of VaRAV1 in grape cells improved its cold tolerance. The transgenic cells exhibited low electrolyte leakage and malondialdehyde (MDA) content and high peroxidase (POD) activity. Moreover, the TF gene TCP8 and a gene involving in homogalacturonan biosynthesis were found to be regulated by VaRAV1, suggesting that the contribution of VaRAV1 to cold tolerance may be achieved by enhancing stability of cell membrane and regulating the expression of target genes involved in plant cell wall composition. Our work provides novel insights into plant response to cold stress and demonstrates the utility of ATAC-seq and RNA-seq for rapid identification of TFs in response to cold stress in grapevine. The VaRAV1 may play an important role in adaption to cold stress.
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http://dx.doi.org/10.1093/pcp/pcab115DOI Listing
July 2021

Differentiation of Multiple Mechanical Stimuli by a Flexible Sensor Using a Dual-Interdigital-Electrode Layout for Bodily Kinesthetic Identification.

ACS Appl Mater Interfaces 2021 Jun 25;13(22):26394-26403. Epub 2021 May 25.

CAS Center for Excellence in Nanoscience, Beijing Key Laboratory of Micro-Nano Energy and Sensor, Beijing Institute of Nanoenergy and Nanosystems, Chinese Academy of Sciences, Beijing 101400, China.

Human bodily kinesthetic sensing is generally complicated and ever-changing due to the diversity of body deformation as well as the complexity of mechanical stimulus, which is different from the unidirectional mechanical motion. So, there exists a huge challenge for current flexible sensors to accurately differentiate and identify what kind of external mechanical stimulus is exerted via analyzing digital signals. Here, we report a flexible dual-interdigital-electrode sensor (FDES) that consists of two interdigital electrodes and a highly pressure-sensitive porous conductive sponge. The FDES can precisely identify multiple mechanical stimuli, e.g., pressing, positive bending, negative bending, -direction stretching, and -direction stretching, and convert them into corresponding current variation signals. Moreover, the FDES exhibits other exceptional properties, such as high sensitivity, stretchability, large measurement range, and outstanding stability, accompanied by simple structural design and low-cost processing simultaneously. Additionally, our FDES successfully identifies various complex activities of the human body, which lays a foundation for the further development of multimode flexible sensors.
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http://dx.doi.org/10.1021/acsami.1c05572DOI Listing
June 2021

All-Fabric Ultrathin Capacitive Sensor with High Pressure Sensitivity and Broad Detection Range for Electronic Skin.

ACS Appl Mater Interfaces 2021 May 12;13(20):24062-24069. Epub 2021 May 12.

Beijing Institute of Nanoenergy and Nanosystems, Chinese Academy of Sciences, Beijing 100083, China.

Flexible pressure sensors have emerged as an indispensable part of wearable devices due to their application in physiological activity monitoring. To realize long-term on-body service, they are increasingly required for properties of conformability, air permeability, and durability. However, the enhancement of sensitivity remains a challenge for ultrathin capacitive sensors, particularly in the low-pressure region. Here, we introduced a highly sensitive and ultrathin capacitive pressure sensor based on a breathable all-fabric network with a micropatterned nanofiber dielectric layer, an all-fabric capacitive sensor (AFCS). This all-fabric network endows a series of exceptional performances, such as high sensitivity (8.31 kPa under 1 kPa), ultralow detection limit (0.5 Pa), wide detection range (0.5 Pa to 80 kPa), and excellent robustness (10 000 dynamic cycles). Besides, the all-fabric structure provides other properties for the AFCS, e.g., high skin conformability, super thinness (dozens of micrometers), and exceptional air permeability. Our AFCS shows promising potential in breathing track, muscle activity detection, fingertip pressure monitoring, and spatial pressure distribution, paving way for comfortable skinlike epidermal electronics.
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http://dx.doi.org/10.1021/acsami.1c05478DOI Listing
May 2021

Neutrophil gelatinase-associated lipocalin: a potential predictor of embolic events in endocarditis.

Ann Thorac Surg 2021 May 4. Epub 2021 May 4.

Department of Cardiac Surgery, The First Affiliated Hospital of Sun Yat-Sen University, Guangzhou 510080, China. Electronic address:

Background: As the complication of infective endocarditis (IE), embolic events (EE) are associated with increased mortality and morbidity. However, there are no reliable indicators to predict embolism. The aim of this study was to evaluate neutrophil gelatinase-associated lipocalin (NGAL) as a biomarker to identify IE patients at high risk of embolism.

Methods: We conducted a prospective observational study of IE patients from January 2018 to December 2020. Plasma NGAL levels were measured in 88 IE patients (37 patients with EE and 51 patients without EE), 91 noninfectious heart valve disease (HVD) patients, and 20 healthy blood donors by ELISA. Native valve tissue was obtained from 16 IE patients and 16 HVD patients. Western blot and immunohistochemical staining were performed to detect NGAL and matrix metalloproteinas-9 (MMP-9).

Results: Higher levels of NGAL were observed in IE patients than HVD patients (P<0.001) or healthy blood donors (P<0.01). In addition, NGAL levels were higher in IE patients with EE compared to those without EE (P<0.001). Receiver operating characteristic analysis demonstrated that NGAL acted as a potential EE predictor with the cutoff value of 166.78 ng/ml. IE patients with higher NGAL levels were significantly related to more severe native valve morphologic changes. NGAL was co-localized with MMP-9, and their expression in the valves of IE patients was higher than those of HVD patients.

Conclusions: NGAL is a potential predictor of EE in IE. This may be attributed to its potency of increasing the proteolytic activity of MMP-9, which leads to valve morphologic impairment.
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http://dx.doi.org/10.1016/j.athoracsur.2021.04.060DOI Listing
May 2021

Melatonin ameliorates myocardial injury by reducing apoptosis and autophagy of cardiomyocytes in a rat cardiopulmonary bypass model.

PeerJ 2021 15;9:e11264. Epub 2021 Apr 15.

Department of Cardiac Surgery, First Affiliated Hospital of Sun Yat-Sen University, Guangzhou, China.

Background: Myocardial injury is a frequent complication after cardiac surgery with cardiopulmonary bypass (CPB). This study aimed to test the hypothesis that melatonin could attenuate myocardial injury in a rat CPB model.

Methods: Eighteen male Sprague-Dawley rats were randomly divided into three groups,  = 6 for each group: the sham operation (SO) group, CPB group and melatonin group. Rats in the SO group underwent cannulation without CPB, rats in CPB group intraperitoneal injected an equal volume of vehicle daily for 7 days before being subjected to CPB and rats in melatonin group intraperitoneal injected 20 mg/kg of melatonin solution daily for 7 days before being subjected to CPB. After 120 min for CPB, the expression levels of plasma interleukin (IL) -6, IL-1β, superoxide dismutase (SOD), glutathione peroxidase (GSH-Px), malondialdehyde (MDA), creatine kinase (CK) -MB and cardiac troponin T (cTnT) were measured. Reactive oxygen species (ROS) were detected by dihydroethidium (DHE). Apoptosis was detected by terminal deoxynucleotidyl transferase dUTP nick-end labeling (TUNEL) staining. Mitochondrial damage and autophagosomes were detected by electron microscopy. Apoptosis inducing factor (AIF) was detected by immunofluorescence. The expression of B cell lymphoma/leukemia2 associated X (Bax), B cell lymphoma/leukemia 2 (Bcl-2), cytochrome C (Cyto-C), cleaved caspase-9, AKT, p-AKT, signal transducer and activator of transcription 3 (STAT3), p-STAT3, LC3, P62, mechanistic target of rapamycin kinase (mTOR), p-mTOR and glyceraldehyde-3-phosphate dehydrogenase (GAPDH) were determined using western blotting.

Results: Melatonin significantly decreased the levels of IL-1β, IL-6, MDA, CK-MB and cTnT and increased the levels of SOD and GSH-Px, all of which were altered by CPB. Melatonin reduced cardiomyocyte superoxide production, the apoptosis index and autophagy in cardiomyocytes induced by CPB. The AKT, STAT3 and mTOR signaling pathways were activated by melatonin during CPB.

Conclusion: Melatonin may serve as a cardioprotective factor in CPB by inhibiting oxidative damage, apoptosis and autophagy. The AKT, STAT3 and mTOR signaling pathways were involved in this process.
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http://dx.doi.org/10.7717/peerj.11264DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8053380PMC
April 2021

Alternatively Expressed Transcripts Analysis of Non-Small Cell Lung Cancer Cells under Different Hypoxic Microenvironment.

J Oncol 2021 10;2021:5558304. Epub 2021 Apr 10.

Department of Cardiac Surgery, The First Affiliated Hospital of Sun Yat-sen University, Guangzhou, China.

Globally, non-small cell lung cancer (NSCLC) is the most fatal form of malignancy. Numerous studies have shown that people living at high altitudes are at a higher risk for cancer. Hypoxia is one of the most important features in high altitude area. Compared with normal cells, cancer cells are more adapted to hypoxia atmosphere. However, at high altitudes, hypoxic conditions are also accompanied by other altered environmental conditions. To identify the single influence of hypoxia, we performed second-generation sequencing to identify gene expression changes triggered by the different oxygen concentrations. We identified 782 genes in A549 cells and 1122 genes in H520 cells that showed altered expression by the combined analysis in 5% oxygen concentration group and 1% oxygen concentration group control group. We further analyzed these targets and found 113 genes altered in both cell lines. Interestingly, we found KxD1 was the only one in both top 10 lists. Further analysis revealed KxD1 to be significantly elevated in NSCLC patients and negatively correlated with prognosis in stage I and II NSCLC patients. Moreover, this correlation reversed in stage III patients. Additionally, compared with patients who only received clean margin operation or chemotherapy, patients who received radiotherapy also showed opposite result. Thus, may be a promising target for the treatment of NSCLC in high-altitude areas.
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http://dx.doi.org/10.1155/2021/5558304DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8055392PMC
April 2021

Transcriptomic analysis of grapevine Dof transcription factor gene family in response to cold stress and functional analyses of the VaDof17d gene.

Planta 2021 Feb 1;253(2):55. Epub 2021 Feb 1.

Beijing Key Laboratory of Grape Science and Enology, and CAS Key Laboratory of Plant Resources, Institute of Botany, Innovation Academy for Seed Design, Chinese Academy of Science, Beijing, 100093, People's Republic of China.

Main Conclusion: Dof genes enhance cold tolerance in grapevine and VaDof17d is tightly associated with the cold-responsive pathway and with the raffinose family oligosaccharides. DNA-binding with one finger (Dof) proteins comprise a large family that plays important roles in the regulation of abiotic stresses. No in-depth analysis of Dof genes has been performed in the grapevine. In this study, we analyzed a total of 25 putative Dof genes in grapevine at genomic and transcriptomic levels, compiled expression profiles of 11 selected VaDof genes under cold stress and studied the potential function of the VaDof17d gene in grapevine calli. The 25 Dof proteins can be classified into four phylogenetic groups. RNA-seq and qRT-PCR results demonstrated that a total of 11 VaDof genes responded to cold stress. Comparative mRNA sequencing of 35S::VaDof17d grape calli showed that VaDof17d was tightly associated with the cold-responsive pathway and with the raffinose family oligosaccharides (RFOs), as observed by the up-regulation of galactinol synthase (GolS) and raffinose synthase genes. We found that the Dof17d-ED (CRISPR/Cas9-mediated mutagenesis of Dof17d-ED) mutant had low cold tolerance with a decreased RFOs level during cold stress. These results formed the fundamental knowledge for further analysis of the biological roles of Dof genes in the grapevine's adaption to cold stresses.
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http://dx.doi.org/10.1007/s00425-021-03574-8DOI Listing
February 2021

M2b macrophages protect against myocardial remodeling after ischemia/reperfusion injury by regulating kinase activation of platelet-derived growth factor receptor of cardiac fibroblast.

Ann Transl Med 2020 Nov;8(21):1409

Department of Cardiac Surgery, First Affiliated Hospital, Sun Yat-sen University, Guangzhou, China.

Background: Myocardial injury is a major cause of myocardial remodeling. Macrophages are important in cardiac repair as a result of their interactions with fibroblasts. As regulatory macrophages, M2b macrophages modulate inflammatory immune responses without participating in wound healing and could have enhanced protective effects on myocardial remodeling. Therefore, we tested the hypothesis that M2b macrophages could improve cardiac function and ameliorate myocardial fibrosis after the myocardial ischemia/reperfusion injury (MI/RI).

Methods: , MI/RI models were established with Sprague-Dawley (SD) rats and either M2b macrophages (MT group) or the same volume of vehicle (CK group) was injected into the ischemic zone. Two weeks after the operation, cardiac function and diameters were determined by echocardiography examination. Level of myocardial fibrosis was measured by Sirius red staining and the expression of fibrosis-related factors. , cardiac fibroblasts (CFs) were co-cultured with M2b macrophages or cultured with M2b macrophage supernatant. Expression of α-smooth muscle actin (α-SMA) and connective tissue growth factor (CCN2/CTGF) in the CFs were measured by western blotting and immunofluorescence staining. In addition, the expression of platelet-derived growth factors (PDGFs), the expression of platelet-derived growth factor receptors (PDGFRs) and the phosphorylation of PDGFRs was detected by western blotting.

Results: A significantly higher rat survival rate, improved left ventricular (LV) systolic function, decreased diameter of the LV and alleviated myocardial fibrosis were observed in the MT group than in the CK group. , the activation of CFs was significantly reduced by the M2b macrophages treatments, relative to the blank control. In addition, the kinase activation of PDGFRs was decreased by M2b macrophage treatments both and .

Conclusions: Our study demonstrated that the administration of M2b macrophages could attenuate myocardial remodeling after MI/RI. The regulation of the activation of PDGFRs in CFs is an important part of the protective mechanism.
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http://dx.doi.org/10.21037/atm-20-2788DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7723613PMC
November 2020

Characterization of mRNA Profiles of Exosomes from Diverse Forms of M2 Macrophages.

Biomed Res Int 2020 21;2020:1585306. Epub 2020 Nov 21.

Department of Cardiac Surgery, The First Affiliated Hospital of Sun Yat-sen University, China.

Exosomes transmit certain amounts of molecules to specific recipient cells for intercellular communication. Among these molecules, messenger RNAs (mRNAs) may be delivered and translated into proteins in the recipient cells, and these mRNAs are thought to be critical mediators of exosomal functions. There are three subtypes of M2 macrophages (MФ), MФ, MФ, and MФ, which have different specific functional programs. The aim of the present study was to screen the mRNA profiles in the exosomes of these macrophage subtypes and to analyze the transcriptomic profile features associated with their specific functions. The mRNA contents of the exosomes isolated from the culture supernatants of the MФ subtypes were analyzed and compared using the Illumina HiSeq platform. The results indicated that the exosomes contained particular mRNAs from their source cells and were messengers of cellular functions. Bioinformatics analysis suggested that the exosomal mRNAs from MФs are enriched in the Toll-like receptor (TLR), tumor necrosis factor (TNF), NOD-like receptor (NLR), and NF-kappa B (NF-B) signaling pathways. The mRNA profile of exosomes from MФ was distinctly different from that of exosomes from MФ and MФ and was consistent with the MФ cytological characteristic of maintaining a high level of proinflammatory cytokine and regulatory factor production. Therefore, the mRNA profiles revealed several characteristics of the exosomes from diverse forms of MФ. Further functional investigations based on these results may advance the understanding of the physiological roles of exosome-transferred mRNAs in MФ functions.
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http://dx.doi.org/10.1155/2020/1585306DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7704128PMC
June 2021

Coding line structured light based on a line-scan camera and its calibration.

Opt Express 2020 Aug;28(17):24799-24812

In a conventional three-dimensional (3D) measurement technique of a line-scan camera, the projection system based on surface structured light is a compromise of traditional projection technology, which suffers from complex calibration, complex structure and low accuracy. To this end, the coding line structured light based on the coded line laser projection system is proposed to address the 3D measurement of a line-scan camera. The single-line projection and codeable characteristics of coded line laser projection system (constructed by a point laser and a micro-electro-mechanical system (MEMS) scanning galvanometer and modeled as the line projection model) are fully matched with the imaging mode of the line-scan camera. The 3D measurement model based on the height information, lateral information and absolute phase of the coding line structured light is derived. The multi-position flat display calibration method is proposed to calibrate the system parameters. In addition, in order to obtain the accurate absolute phase from the phase shift combined binary code, the periodic error correction method based on expansion-corrosion is proposed to correct the phase error. Contrary to conventional structured light methods based on a line-scan camera, the proposed method has the advantages of high measurement accuracy, high efficiency, more compactness and low cost. The experiments affirm that the coding line structured light is valid and the proposed calibration method is feasible. Experimental results also indicate that the proposed method performs well for both diffuse reflective surfaces and reflective surfaces that are difficult to measure with conventional structured light methods based on a line-scan camera.
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http://dx.doi.org/10.1364/OE.398961DOI Listing
August 2020

Sensitive and Stable 2D Perovskite Single-Crystal X-ray Detectors Enabled by a Supramolecular Anchor.

Adv Mater 2020 Oct 2;32(40):e2003790. Epub 2020 Sep 2.

State Key Laboratory of Supramolecular Structure and Materials, College of Chemistry, Jilin University, Changchun, 130012, P. R. China.

Perovskite X-ray detectors have been demonstrated to be sensitive to soft X-rays (<80 keV) for potential medical imaging applications. However, developing X-ray detectors that are stable and sensitive to hard X-rays (80 to 120 keV) for practical medical imaging is highly desired. Here, a sensitive 2D fluorophenethylammonium lead iodide ((F-PEA) PbI ) perovskite single-crystal hard-X-ray detector from low-cost solution processes is reported. Dipole interaction of organic ions promotes the ordering of benzene rings as well as the supramolecular electrostatic interaction between electron-deficient F atoms with neighbor benzene rings. Supramolecular interactions serve as a supramolecular anchor to stabilize and tune the electronic properties of single crystals. The 2D (F-PEA) PbI perovskite single crystal exhibits an intrinsic property with record bulk resistivity of 1.36 × 10 Ω cm, which brings a low device noise for hard X-ray detection. Meanwhile, the ion-migration phenomenon is effectively suppressed, even under the large applied bias of 200 V, by blocking the ion migration paths after anchoring. Consequently, the (F-PEA) PbI single crystal detector yields a sensitivity of 3402 μC Gy air cm to 120 keV hard X-rays with lowest detectable X-ray dose rate of 23 nGy s , outperforming the dominating CsI scintillator of commercial digital radiography systems by acquiring clear X-ray images under much lower dose rate. In addition, the detector shows high operation stability under extremely high-flux X-ray irradiation.
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http://dx.doi.org/10.1002/adma.202003790DOI Listing
October 2020

Conditioned medium from M2b macrophages modulates the proliferation, migration, and apoptosis of pulmonary artery smooth muscle cells by deregulating the PI3K/Akt/FoxO3a pathway.

PeerJ 2020 5;8:e9110. Epub 2020 May 5.

Department of Cardiac Surgery, The First Affiliated Hospital of Sun Yat-sen University, Guangzhou, China.

Background: Immunity and inflammation are considered to be central features of pulmonary artery hypertension (PAH), in which macrophages are one of the main components of inflammatory cell infiltration around the pulmonary artery. M2b macrophages, which are different from M1 and M2 macrophages, are believed to have immunomodulatory activities and produce little fibrosis. The purpose of this study was to explore the effect of M2b macrophages on pulmonary artery smooth muscle cells (PASMCs) derived from monocrotaline-induced PAH rats.

Methods: PASMCs were cultured in serum-free medium, the supernatant of M0 macrophages, or the supernatant of M2b macrophages for 24 hours. Then cell proliferation was assessed by cell counting kit-8 and cell migration ability was detected by wound healing and transwell assays. The apoptosis rate of cells was determined by TUNEL staining and annexin V-PE/7-ADD staining. Western blot was used to detect the expression of Bcl-2 family proteins, cleaved caspase-9 and PI3K/Akt/FoxO3a pathway. LY294002 (a specific inhibitor of PI3K) was used to investigate its effect on PASMCs and its relationship with M2b macrophages.

Results: Conditioned medium from M2b macrophages significantly inhibited the proliferation and migration of PASMCs compared with the control group and M0 macrophage group. Furthermore, conditioned medium from M2b macrophages promote PASMC apoptosis and increased the expression of pro-apoptotic proteins Bax and cleaved caspase-9, inhibited the expression of anti-apoptotic proteins Bcl-2 and Bcl-xl. Finally, conditioned medium from M2b macrophages inhibited the PI3K/Akt/FoxO3a pathway. Inhibition of PI3K/Akt/FoxO3a pathway also significantly inhibit the proliferation, migration, and apoptosis resistance of PASMCs.

Conclusion: Conditioned medium from M2b macrophages can inhibit the proliferation, migration, and apoptosis resistance of PASMCs, which may be at least partially by deregulating the PI3K/Akt/FoxO3a pathway.
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http://dx.doi.org/10.7717/peerj.9110DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7207208PMC
May 2020

Facile Strategy for Facet Competition Management to Improve the Performance of Perovskite Single-Crystal X-ray Detectors.

J Phys Chem Lett 2020 May 22;11(9):3529-3535. Epub 2020 Apr 22.

State Key Laboratory of Supramolecular Structure and Materials, College of Chemistry, Jilin University, Changchun 130012, P. R. China.

Methylammonium lead tribromide perovskite single crystals have been demonstrated to be good candidates as sensitive X-ray detectors in direct detection mode in recent years. However, its X-ray detection performance based on the orientation of different facets is still not clear. Here, we developed a facile strategy to chemically expose the [110] facet of single crystals from low-cost solution processes by tailoring the nonstoichiometry of feeding ions to selectively suppress the growth of the [100] facet. In contrast to physically cutting and sawing single-crystal ingots, this avoids damage to the fragile single crystals as well as orientation errors, more suitable for the naturally soft lattice. Compared to the [100] facet, the exposed [110] facet of perovskite single crystals exhibits a smaller trap density and excellent charge carrier transportation properties, leading to an improved sensitivity of 3928.3 μC/Gy/cm to 120 keV hard X-rays, which potentially outperforms the currently dominating CsI scintillator of a commercial digital radiography (DR) medical imager for a routine health check.
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http://dx.doi.org/10.1021/acs.jpclett.0c00770DOI Listing
May 2020

M2b Macrophages Regulate Cardiac Fibroblast Activation and Alleviate Cardiac Fibrosis After Reperfusion Injury.

Circ J 2020 03 10;84(4):626-635. Epub 2020 Mar 10.

Department of Cardiac Surgery, The First Affiliated Hospital of Sun Yat-Sen University.

Background: Macrophages play an important role in the development of cardiac fibrosis. However, the roles of different macrophage subtypes in cardiac fibroblast (CF) activation and cardiac fibrosis are unknown.Methods and Results:Bone marrow-derived macrophages (BMDMs) were treated with different stimuli to induce differentiation into M1, M2a, M2b, and M2c macrophage subtypes. CFs were co-cultured with different subtypes of macrophages or cultured with macrophage supernatants. Results revealed that M2b macrophages significantly suppressed the proliferation and migration of CFs, the expression of fibrosis-related proteins (collagen I [COL-1] and α-smooth muscle actin [α-SMA]), and differentiation into cardiac myofibroblasts (MFs). The opposite effects were observed with M2a macrophages. A rat model of cardiac ischemia/reperfusion (I/R) injury was used to determine the effect of M2b macrophages transplantation. After cardiac I/R injury, transplantation of M2b macrophages improved cardiac function and reduced cardiac fibrosis. The effect of macrophage subtypes on p-ERK, ERK, p-p38, and p38 phosphorylation was examined by Western blotting. The results showed that M2b macrophages significantly inhibited the mitogen-activated protein kinase (MAPK) signaling pathway.

Conclusions: These study results demonstrate for the first time that different subtypes of macrophages have different roles in regulating CF activation. M2b macrophages inhibit CF activation, and thus can be considered anti-fibrotic macrophages. M2a macrophages promote CF activation, and thus are pro-fibrotic macrophages.
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http://dx.doi.org/10.1253/circj.CJ-19-0959DOI Listing
March 2020

Does an imbalance in circulating vascular endothelial growth factors (VEGFs) cause atrial fibrillation in patients with valvular heart disease?

J Thorac Dis 2019 Dec;11(12):5509-5516

Department of Cardiac Surgery, The First Affiliated Hospital of Sun Yat-sen University, Guangzhou 510080, China.

Background: The pathogenesis of atrial fibrillation (AF) remains unclear. Vascular endothelial growth factors (VEGFs) can stimulate fibrosis within the atrium and ventricle. We hypothesized that there is a relationship between the serum VEGFs/soluble vascular endothelial growth factor receptor (sVEGFRs) levels and AF in patients with valvular heart disease (VHD). This provides a new paradigm for studying AF.

Methods: The plasma levels of VEGF-A, VEGF-C, sVEGFR-1 and sVEGFR-2 were detected by enzyme-linked immunosorbent assay (ELISA). A total of 100 people, consisting of AF patients (long-standing, persistent AF; n=49), sinus rhythm (SR) patients (n=31) and healthy controls (n=20), were included in this study.

Results: The plasma levels of VEGF-A were significantly higher in AF patients compared to healthy control (P<0.05). The plasma levels of sVEGFR-1 were significantly higher in AF compared to SR (P<0.05). The plasma levels of sVEGFR-2 were significantly lower in AF patients compared to SR patients and healthy controls (both P<0.05). There was a significant and negative correlation between AF and the sVEGFR-2 levels in the groups (r=-0.432, P=0.000).

Conclusions: An imbalance in VEGFs and sVEGFRs may contribute to AF by breaking the balance of angiogenesis and lymphangiogenesis. Additionally, sVEGFR-2 may be an important biomarker of AF.
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http://dx.doi.org/10.21037/jtd.2019.11.32DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6988013PMC
December 2019

Stretchable Hybrid Bilayered Luminescent Composite Based on the Combination of Strain-Induced and Triboelectrification-Induced Electroluminescence.

ACS Omega 2019 Dec 25;4(24):20470-20475. Epub 2019 Nov 25.

CAS Center for Excellence in Nanoscience, Beijing Key Laboratory of Micro-Nano Energy and Sensor, Beijing Institute of Nanoenergy and Nanosystems, and Institute of Semiconductors, Chinese Academy of Sciences, Beijing 100083, China.

High luminescence intensity from materials that are excited by external stimuli is highly desired. In this work, a stretchable hybrid luminescent composite (HLC) that has multiple luminescence modes is reported. The luminescence can be excited either by externally applied mechanical strain or by a moving object that slides against the HLC. When the HLC is deformed, such as being twisted or folded, the ZnS/Cu phosphor experiences mechanical strain that trigger the mechanoluminescence (ML) of the phosphors. Moreover, as the HLC slides against a contact object, the triboelectrification at the contact interface induces the electroluminescence of phosphor. Here, a series of internal and external factors were studied on how they influence the luminescent intensity. It is found that the luminescent intensity from the two modes can be superposed. The HLC material was used to fabricate a fiber-based luminescent device that can be driven by air flow. The overall luminescent intensity is enhanced by over 72% compared to that obtained solely from the ML. The HLC reported in this work has such potential applications as self-powered light sources and sensors as means of detecting dynamic motions and interaction.
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http://dx.doi.org/10.1021/acsomega.9b01717DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6906773PMC
December 2019

PCIR: a database of Plant Chloroplast Inverted Repeats.

Database (Oxford) 2019 01;2019

Agricultural Big-Data Research Center and College of Plant Protection, Shandong Agricultural University, Tai'an 271018, China.

Inverted repeats (IRs) serve as potential biomarkers for genomic instability, DNA replication and other genetic processes. However, little information can be found in databases to help researchers recognize potential IR nucleotides, explore junction sites and annotate related functional genes. Plant Chloroplast Inverted Repeats (PCIR) is an interactive, web-based platform containing various sequenced chloroplast genomes that enables detection, searching and visualization of large-scale detailed information on IRs. PCIR contains many datasets, including 21 433 IRs, 113 plants chloroplast genomes, 16 948 functional genes and 21 659 visual maps. This database offers an online prediction tool for detecting IRs based on DNA sequences. PCIR can also analyze phylogenetic relationships using IR information among different species and provide users with high-quality marker maps. This database will be a valuable resource for IR distribution patterns, related genes and architectural features.
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http://dx.doi.org/10.1093/database/baz127DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6835207PMC
January 2019

Modifying Electrical and Magnetic Properties of Single-Walled Carbon Nanotubes by Decorating with Iron Oxide Nanoparticles.

J Nanosci Nanotechnol 2020 04;20(4):2611-2616

Department of Electrical and Computer Engineering, Center for Materials for Information Technology, The University of Alabama, Tuscaloosa, AL 35487, USA.

In this work, we report a facile approach to modify the magnetic and electrical property of Hipco single-walled carbon nanotubes (SWCNTs) by attaching iron oxide nanoparticles (FeNPs). The Raman spectra of FeNP-SWCNTs revealed an apparent intensity reduction of metallic peaks in Radial breathing mode (RBM) region, which indicates the ratio change between the metallic and semiconducting component of SWCNTs after modification. Distinctive magnetic and electric resistance properties of FeNP-SWCNTs were found to be associated with the different shapes of FeNPs. The specific FeNP examples as showcased in this work can be applied to tune the electric and magnetic properties of other nanostructured materials in general and shed light on various electronic and magnetic applications.
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http://dx.doi.org/10.1166/jnn.2020.17215DOI Listing
April 2020

High-Efficiency and Stable Perovskite Solar Cells Prepared Using Chlorobenzene/Acetonitrile Antisolvent.

ACS Appl Mater Interfaces 2019 Sep 12;11(38):34989-34996. Epub 2019 Sep 12.

State Key Laboratory on Integrated Optoelectronics and College of Electronic Science & Engineering , Jilin University , 2699 Qianjin Street , Changchun 130012 , P. R. China.

Preparing high-quality perovskite film with large grain size and fewer trap states is of vital importance in boosting the efficiency and stability of perovskite solar cells (PSCs). However, it is still difficult to obtain perfect MAPbI films by antisolvent treatment so far because of the small grain size, pinholes, and numerous defects in perovskite layers. Herein, acetonitrile (ACN) was introduced into chlorobenzene (CB) antisolvent to modify the MAPbI active layer. The results show that the ACN could control the ratio of the DMSO in MAI-PbI-DMSO intermediate phase film effectively and thus manipulate the formation of MAPbI film. Relatively high-quality perovskite films with larger grain size were obtained when we added 6% v/v ACN into CB antisolvent. Based on the ACN-modified MAPbI film, the n-i-p planar device with the structure of FTO/SnO/MAPbI/spiro-OMeTAD/Ag yields the best power conversion efficiency (PCE) of 18.9%. It exhibited an enhancement of 16.6% in efficiency compared with the PCE of 16.2% for the control device. In addition, the device based on ACN-modified MAPbI also presents improved stability in air atmosphere.
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http://dx.doi.org/10.1021/acsami.9b12323DOI Listing
September 2019

Genome-Wide Identification and Characterization of Xyloglucan Endotransglycosylase/Hydrolase in during Development.

Genes (Basel) 2019 07 16;10(7). Epub 2019 Jul 16.

Agricultural Big-Data Research Center and College of Plant Protection, Shandong Agricultural University, Taian 271018, China.

Xyloglucan endotransglycosylase/hydrolase (XTH) is a cell-wall-modifying enzyme participating in diverse cell morphogenetic processes and adaptation to stress. In this study, 48 XTH genes were identified from two pineapple () cultivars ('F153' and 'MD2') and designated to and to based on their orthology with genes. Endoglucanase family 16 members were identified in addition to XTHs of glycoside hydrolase family 16. Phylogenetic analysis clustered the XTHs into three major groups (Group I/II, III and Ancestral Group) and Group III was subdivided into Group IIIA and Group IIIB. Similar gene structure and motif number were observed within a group. Two highly conserved domains, glycosyl hydrolase family 16 (GH16-XET) and xyloglucan endotransglycosylase C-terminus (C-XET), were detected by multiple sequences alignment of all XTHs. Segmental replication were detected in the two cultivars, with only the paralogous pair presented in 'F153' prior to genomic expansion. Transcriptomic analysis indicated that XTHs were involved in the regulation of fruit ripening and crassulacean acid metabolism with tissue specificity and quantitative real-time PCR analysis suggested that was involved in root growth. The results enhance our understanding of XTHs in the plant kingdom and provide a basis for further studies of functional diversity in .
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http://dx.doi.org/10.3390/genes10070537DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6678617PMC
July 2019

The genome assembly and annotation of yellowhorn (Xanthoceras sorbifolium Bunge).

Gigascience 2019 06;8(6)

College of Plant Protection, Shandong Agricultural University, Daizong Road No.61, Tai'an 271018, China.

Background: Yellowhorn (Xanthoceras sorbifolium Bunge), a deciduous shrub or small tree native to north China, is of great economic value. Seeds of yellowhorn are rich in oil containing unsaturated long-chain fatty acids that have been used for producing edible oil and nervonic acid capsules. However, the lack of a high-quality genome sequence hampers the understanding of its evolution and gene functions.

Findings: In this study, a whole genome of yellowhorn was sequenced and assembled by integration of Illumina sequencing, Pacific Biosciences single-molecule real-time sequencing, 10X Genomics linked reads, Bionano optical maps, and Hi-C. The yellowhorn genome assembly was 439.97 Mb, which comprised 15 pseudo-chromosomes covering 95.42% (419.84 Mb) of the assembled genome. The repetitive fractions accounted for 56.39% of the yellowhorn genome. The genome contained 21,059 protein-coding genes. Of them, 18,503 (87.86%) genes were found to be functionally annotated with ≥1 "annotation" term by searching against other databases. Transcriptomic analysis showed that 341, 135, 125, 113, and 100 genes were specifically expressed in hermaphrodite flower, staminate flower, young fruit, leaf, and shoot, respectively. Phylogenetic analysis suggested that yellowhorn and Dimocarpus longan diverged from their most recent common ancestor ∼46 million years ago.

Conclusions: The availability and subsequent annotation of the yellowhorn genome, as well as the identification of tissue-specific functional genes, provides a valuable reference for plant comparative genomics, evolutionary studies, and molecular design breeding.
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http://dx.doi.org/10.1093/gigascience/giz071DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6593362PMC
June 2019

Plant-GQ: An Integrative Database of G-Quadruplex in Plant.

J Comput Biol 2019 09 8;26(9):1013-1019. Epub 2019 Apr 8.

Agricultural Big-Data Research Center and College of Plant Protection, Shandong Agricultural University, Taian, China.

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http://dx.doi.org/10.1089/cmb.2019.0010DOI Listing
September 2019

Genome-wide identification and expression analysis of HKT transcription factor under salt stress in nine plant species.

Ecotoxicol Environ Saf 2019 Apr 9;171:435-442. Epub 2019 Jan 9.

Beijing Key Laboratory of Grape Science and Enology and Key Laboratory of Plant Resource, Institute of Botany, Chinese Academy of Sciences, Beijing 100093, China. Electronic address:

High-affinity K+ (HKT) gene family is regulated the transport of Na+ and maintain the balance between Na+ and K+ in the process of plant growth, development and response to abiotic stress. Despite this fact, systemic and comprehensive studies on HKT in multiply plants remains unknown. A total of 29 HKT genes distributed on nine species were identified. Phylogenetic tree analysis results indicated that HKT genes were divided into five homology subfamilies. Combining structural analysis with protein contains five highly conservative motifs, HKT family has similar gene structures and special gene characteristics. Finally, the expression patterns of HKT showed two different dramatic changes in different organs and tissues under different salt stress in multiply plants. This study has many implications for research into the comparative genomics analysis of HTK gene family, which revealed regulation mechanism of HKT genes, is valuable for understanding development and response to abiotic stress in plant.
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http://dx.doi.org/10.1016/j.ecoenv.2019.01.008DOI Listing
April 2019

Fully Rollable Lead-Free Poly(vinylidene fluoride)-Niobate-Based Nanogenerator with Ultra-Flexible Nano-Network Electrodes.

ACS Nano 2018 05 1;12(5):4803-4811. Epub 2018 May 1.

CAS Center for Excellence in Nanoscience, Beijing Key Laboratory of Micro-nano Energy and Sensor , Beijing Institute of Nanoenergy and Nanosystems, Chinese Academy of Sciences , Beijing 100083 , China.

A fully rollable nanocomposite-based nanogenerator (NCG) is developed by integrating a lead-free piezoelectric hybrid layer with a type of nanofiber-supported silver nanowire (AgNW) network as electrodes. The thin-film nanocomposite is composed of electroactive polyvinylidene fluoride (PVDF) polymer matrix and compositionally modified potassium sodium niobate-based nanoparticles (NPs) with a high piezoelectric coefficient ( d) of 53 pm/V, which is revealed by the piezoresponse force microscopy measurements. Under periodical agitation at a compressive force of 50 N and 1 Hz, the NCG can steadily render high electric output up to an open-circuit voltage of 18 V and a short-circuit current of 2.6 μA. Of particular importance is the decent rollability of the NCG, as indicated by the negligible decay in the electric output after it being repeatedly rolled around a gel pen for 200 cycles. Besides, the biocompatible NCG can potentially be used to scavenge biomechanical energy from low-frequency human motions, as demonstrated by the scenarios of walking and elbow joint movement. These results rationally expand the feasibility of the developed NCG toward applications in lightweight, diminutive, and multifunctional rollable or wearable electronic devices.
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http://dx.doi.org/10.1021/acsnano.8b01534DOI Listing
May 2018

GAN: a platform of genomics and genetics analysis and application in Nicotiana.

Database (Oxford) 2018 01;2018

Agricultural Big-Data Research Center and College of Plant Protection, Shandong Agricultural University, Taian 271018, China.

Database Url: http://biodb.sdau.edu.cn/gan/.
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http://dx.doi.org/10.1093/database/bay001DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5822692PMC
January 2018

Functional and evolution characterization of SWEET sugar transporters in Ananas comosus.

Biochem Biophys Res Commun 2018 02 4;496(2):407-414. Epub 2018 Jan 4.

Agricultural Big-Data Research Center, College of Information Science and Engineering, College of Plant Protection, Shandong Agricultural University, Taian 271018, China. Electronic address:

Sugars will eventually be exported transporters (SWEETs) are a group of recently identified sugar transporters in plants that play important roles in diverse physiological processes. However, currently, limited information about this gene family is available in pineapple (Ananas comosus). The availability of the recently released pineapple genome sequence provides the opportunity to identify SWEET genes in a Bromeliaceae family member at the genome level. In this study, 39 pineapple SWEET genes were identified in two pineapple cultivars (18 AnfSWEET and 21 AnmSWEET) and further phylogenetically classified into five clades. A phylogenetic analysis revealed distinct evolutionary paths for the SWEET genes of the two pineapple cultivars. The MD2 cultivar might have experienced a different expansion than the F153 cultivar because two additional duplications exist, which separately gave rise to clades III and IV. A gene exon/intron structure analysis showed that the pineapple SWEET genes contained highly conserved exon/intron numbers. An analysis of public RNA-seq data and expression profiling showed that SWEET genes may be involved in fruit development and ripening processes. AnmSWEET5 and AnmSWEET11 were highly expressed in the early stages of pineapple fruit development and then decreased. The study increases the understanding of the roles of SWEET genes in pineapple.
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http://dx.doi.org/10.1016/j.bbrc.2018.01.024DOI Listing
February 2018

Nano-Snowflower of Gold Nanoparticles-Ruthenium Metallopolymer-Carbon Nanotubes Binding Anti-DNP IgE Antibody.

J Nanosci Nanotechnol 2015 Aug;15(8):5733-40

Gold nanoparticles (AuNPs) have been chemically functionalized onto multiwalled carbon nanotubes (MWCNT) through a metallopolymer linker-bis (2,2':6'2"-terpyridine) ruthenium(II)-connected diblock poly(N-isopropyacryamide). A "nano-snowflower" pattern was formed by self-assembly MWCNT-AuNP nanocomposite with anti-DNP IgE antibody. MWCNT-AuNP nanohybrid has unique biocompatibility and electronic current-voltage properties. This nanohybrid shows the potential application for IgE biosensor to diagnose cancer cells. We represent a step towards building complex electronic circuits response by providing molecular recognition properties.
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http://dx.doi.org/10.1166/jnn.2015.10303DOI Listing
August 2015

Collision induced dissociations of non-derivatized and trimethylsilyl-derivatized estradiols: similarities in fragmentation patterns.

J Mass Spectrom 2015 Feb;50(2):308-15

Department of Chemistry and Biochemistry, Spelman College, 350 Spelman Lane, Box 1134, Atlanta, GA, 30314, USA.

Fragmentation mechanisms of estradiol and trimethylsilyl (TMS)-derivatized estradiol were studied by triple quadrupole tandem mass spectrometry (MSMS) and density functional theory (DFT) at B3LYP/6-311G(d,p) level. Collision induced dissociations (CID) of estradiol give product ions that are associated with the cleavage of B, C and D rings. Characteristic fragments from the cleavage of the aromatic ring A were not identified, and this was confirmed with both labeled estradiol and trimethylsilyl (TMS)-derivatized estradiol. The mechanisms are based on charge-site directed, radical-directed and charge remote fragmentations that are consistent with previous studies of steroids. CID spectra show ion pairs at m/z: 145/146, 157/158, 185/186, 211/213 and 225/226 with significant intensities, suggesting that these pairs are not from isotopic contributions. The mechanisms show similarities with some minor differences in the fragmentation patterns between the non-derivatized and the TMS-derivatized estradiol.
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http://dx.doi.org/10.1002/jms.3529DOI Listing
February 2015
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