Publications by authors named "Huaqing Liu"

79 Publications

Inorganic particle accumulation promotes nutrient removal of vertical flow constructed wetlands: Mechanisms and implications.

Sci Total Environ 2021 Mar 3;778:146203. Epub 2021 Mar 3.

Shandong Key Laboratory of Water Pollution Control and Resource Reuse, School of Environmental Science & Engineering, Shandong University, Qingdao 266237, PR China. Electronic address:

Vertical flow constructed wetlands (VF CWs) are widely applied for treating eutrophic water due to prominent advantages in economy and ecology. Natural inorganic particles are ubiquitous in contaminated water and the accumulation of inorganic particles takes place spontaneously in VF CWs. To reveal how the accumulation of inorganic particles affects the transport and transformation of phosphorus (P) and nitrogen (N) in VF CWs, column experiments with and without inorganic particle loading were conducted for over 180 days. The morphology and mass balance of P and N, microbial community structure and hydraulic characteristics of VF CWs were investigated. The average total phosphorus (TP) and total nitrogen (TN) removal efficiencies in VF CWs with inorganic particle loading were steady at 90.4 ± 1.9% and 87.8 ± 2.3%, respectively. Inorganic particle accumulation improved TP removal mainly via adsorption and plant uptake, while enhanced TN removal was mainly attributed to higher plant uptake and microbial degradation. Of particular interest was that plant biomass production was doubled by the concentrated nutrients (e.g., bioavailable P and N) in the rhizosphere, accompanied by the accumulation of inorganic particles up to 9.5 g L. Accumulated particles increased the bacterial abundance by 7.7-fold, and the diversity of the bacterial community associated with P and N transformations was significantly enhanced (p < 0.05). P NMR and P fractionation revealed that the elevated P proportion in the substrate was mainly in the form of iron-bound inorganic P. Moreover, inorganic particle accumulation decreased the substrate hydraulic conductivity, while it showed limited effect on the reduction of the hydraulic retention time.
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http://dx.doi.org/10.1016/j.scitotenv.2021.146203DOI Listing
March 2021

Performance and bacterial community dynamics of hydroponically grown Iris pseudacorus L. during the treatment of antibiotic-enriched wastewater at low/normal temperature.

Ecotoxicol Environ Saf 2021 Apr 11;213:111997. Epub 2021 Feb 11.

College of Life Science, Cangzhou Normal University, Cangzhou 061001, PR China.

Antibiotics are widely detected in the water environment, posing a serious threat to the health of humans and animals. The effect of levofloxacin (LOFL) on pollutant removal and the difference in the influence mechanisms at normal and low temperatures in constructed wetlands are worth discussing. A hydroponic culture experiment was designed with Iris pseudacorus L. at low and normal temperatures. LOFL (0-100 µg/L) was added to the systems. The results indicated that the removal of pollutants was affected most by temperature, followed by LOFL concentration. At the same concentration of LOFL, the pollutant removal rate was significantly higher at normal temperature than at low temperature. Low concentrations of LOFL promoted the degradation of pollutants except TN under normal-temperature conditions. Compared with the results at low temperature, the bacterial community richness was higher and the diversity of bacterial communities was lower under normal-temperature conditions. The genera and the function of bacteria were greatly affected by antibiotic concentration, temperature and test time. A series of microorganisms resistant to antibiotics and low temperature were identified in this study. The results will provide valuable information and a reference for our understanding of the ecological effects of LOFL.
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http://dx.doi.org/10.1016/j.ecoenv.2021.111997DOI Listing
April 2021

The association of eating behaviors with blood pressure levels in college students: a cross-sectional study.

Ann Transl Med 2021 Jan;9(2):155

Centre for Health Management and Policy Research, School of Public Health, Cheeloo College of Medicine, Shandong University, Jinan, China.

Backgrounds: The present study aimed to investigate the relationship between eating habits and blood pressure levels in college students in order to provide more insights into the prevention and control of hypertension.

Methods: A self-administered questionnaire was distributed to first-year college students. The demographic characteristics, eating behaviors, smoking and drinking status, and physical activity of 3,324 eligible respondents were analyzed. Multivariate logistics regression model was used to analyze the association of eating behaviors with blood pressure levels.

Results: The study participants had a mean (SD) age of 18.51 (1.00) years. The mean systolic blood pressure (SBP) and diastolic blood pressure (DBP) levels were 114.08 and 70.92 mmHg, respectively. The overall prevalence of hypertension was 7.2%; and the prevalence among males and females was 12.9% and 3.2%, respectively. Multivariate logistic regression results confirmed that students' taste preference, desserts, and late-night snacks were associated with hypertension. Students who ate spicy food had a lower risk of high blood pressure (OR =0.642, P=0.028); as was having dessert 3-6 times a week (OR =0.702, P=0.037), while those who ate late-night snacks on 6-7 days of the week had a higher risk for hypertension (OR =2.093, P=0.013).

Conclusions: More targeted interventions should be taken to improve students' eating habits and control their blood pressure.
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http://dx.doi.org/10.21037/atm-20-8031DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7867881PMC
January 2021

Stable-isotope probing coupled with high-throughput sequencing reveals bacterial taxa capable of degrading aniline at three contaminated sites with contrasting pH.

Sci Total Environ 2021 Jun 27;771:144807. Epub 2021 Jan 27.

National-Regional Joint Engineering Research Center for Soil Pollution Control and Remediation in South China, Guangdong Key Laboratory of Integrated Agro-environmental Pollution Control and Management, Institute of Eco-environmental and Soil Sciences, Guangdong Academy of Sciences, Guangzhou 510650, China. Electronic address:

The biodegradation of aniline is an important process related to the attenuation of aniline pollution at contaminated sites. Aniline contamination could occur in various pH (i.e., acidic, neutral, and alkaline) environments. However, little is known about preferred pH conditions of diverse aniline degraders at different sites. This study investigated the active aniline degraders present under contrasting pH environments using three aniline-contaminated cultures, namely, acidic sludge (ACID-S, pH 3.1), neutral river sediment (NEUS, pH 6.6), and alkaline paddy soil (ALKP, pH 8.7). Here, DNA-based stable isotope probing coupled with high-throughput sequencing revealed that aniline degradation was associated with Armatimonadetes sp., Tepidisphaerales sp., and Rhizobiaceae sp. in ACID-S; Thauera sp., Zoogloea sp., and Acidovorax sp. in NEUS; Delftia sp., Thauera sp., and Nocardioides sp. in ALKP. All the putative aniline-degrading bacteria identified were present in the "core" microbiome of these three cultures; however, only an appropriate pH may facilitate their ability to metabolize aniline. In addition, the biotic interactions between putative aniline-degrading bacteria and non-direct degraders showed different characteristics in three cultures, suggesting aniline-degrading bacteria employ diverse survival strategies in different pH environments. These findings expand our current knowledge regarding the diversity of aniline degraders and the environments they inhabit, and provide guidance related to the bioremediation of aniline contaminated sites with complex pH environments.
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http://dx.doi.org/10.1016/j.scitotenv.2020.144807DOI Listing
June 2021

Solar-driven, self-sustainable electrolysis for treating eutrophic river water: Intensified nutrient removal and reshaped microbial communities.

Sci Total Environ 2021 Apr 24;764:144293. Epub 2020 Dec 24.

National-Regional Joint Engineering Research Center for Soil Pollution Control and Remediation in South China, Guangdong Key Laboratory of Integrated Agro-environmental Pollution Control and Management, Guangdong Institute of Eco-environmental Science & Technology, Guangdong Academy of Sciences, Guangzhou 510650, China. Electronic address:

River ecosystems are the most important resource of surface freshwater, but they have frequently been contaminated by excessive nutrient input of nitrogen (N) and phosphorus (P) in particular. An efficient and economic river water treatment technology that possesses the capacity of simultaneous N and P removal is urgently required. In this study, a solar-driven, self-sustainable electrolytic treatment was conducted in situ to intensify N and P removal from eutrophic river water. Solar panel was applied to provide the electrolysis setups with energy (voltage 10 ± 0.5 V), and the current density was controlled to be 0.06 ± 0.02 mA cm. Results indicated that the average removal efficiencies of total N (TN) and total P (TP) under electrolysis conditions reached 72.4 ± 11.7 and 13.8 ± 5.3 mg m d, which were 3.7- and 4.7-fold higher compared to untreated conditions. Enhanced TN removal mainly reflected the abatement of nitrate N (NO-N) (80.6 ± 4.1%). The formation of ferric ions through the electro-dissolution of the sacrificial iron anode improved TP removal by coprecipitation with SPS. Combined high-throughput sequencing and statistical analyses revealed that electrolysis significantly reshaped the microbial communities in both the sediment-water interface and suspended sediment (SPS), and hydrogenotrophic denitrifiers (e.g., Hydrogenophaga) were highly enriched under electrolysis conditions. These findings indicated that in situ electrolysis is a feasible and effective technology for intensified nutrient removal from river water.
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http://dx.doi.org/10.1016/j.scitotenv.2020.144293DOI Listing
April 2021

Differentially expressed serum proteins in children with or without asthma as determined using isobaric tags for relative and absolute quantitation proteomics.

PeerJ 2020 3;8:e9971. Epub 2020 Nov 3.

Department of Obstetrics and Gynecology, Maternal and Child Health Hospital, the Affiliated Hospital of Anhui Medical University, Hefei, Anhui, China.

Background: Although asthma is one of the most common chronic, noncommunicable diseases worldwide, the pathogenesis of childhood asthma is not yet clear. Genetic factors and environmental factors may lead to airway immune-inflammation responses and an imbalance of airway nerve regulation. The aim of the present study was to determine which serum proteins are differentially expressed between children with or without asthma and to ascertain the potential roles that these differentially expressed proteins (DEPs) may play in the pathogenesis of childhood asthma.

Methods: Serum samples derived from four children with asthma and four children without asthma were collected. The DEPs were identified by using isobaric tags for relative and absolute quantitation (iTRAQ) combined with liquid chromatography tandem mass spectrometry (LC-MS/MS) analyses. Using biological information technology, including Gene Ontology (GO), Kyoto Encyclopedia of Genes and Genomes (KEGG), and Cluster of Orthologous Groups of Proteins (COG) databases and analyses, we determined the biological processes associated with these DEPs. Key protein glucose-6-phosphate dehydrogenase (G6PD) was verified by enzyme linked immunosorbent assay (ELISA).

Results: We found 46 DEPs in serum samples of children with asthma vs. children without asthma. Among these DEPs, 12 proteins were significantly (>1.5 fold change) upregulated and 34 proteins were downregulated. The results of GO analyses showed that the DEPs were mainly involved in binding, the immune system, or responding to stimuli or were part of a cellular anatomical entity. In the KEGG signaling pathway analysis, most of the downregulated DEPs were associated with cardiomyopathy, phagosomes, viral infections, and regulation of the actin cytoskeleton. The results of a COG analysis showed that the DEPs were primarily involved in signal transduction mechanisms and posttranslational modifications. These DEPs were associated with and may play important roles in the immune response, the inflammatory response, extracellular matrix degradation, and the nervous system. The downregulated of G6PD in the asthma group was confirmed using ELISA experiment.

Conclusion: After bioinformatics analyses, we found numerous DEPs that may play important roles in the pathogenesis of childhood asthma. Those proteins may be novel biomarkers of childhood asthma and may provide new clues for the early clinical diagnosis and treatment of childhood asthma.
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http://dx.doi.org/10.7717/peerj.9971DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7646293PMC
November 2020

Environmental application of magnetic cellulose derived from Pennisetum sinese Roxb for efficient tetracycline removal.

Carbohydr Polym 2021 Jan 1;251:117004. Epub 2020 Sep 1.

College of Natural Resources and Environment, South China Agricultural University, Guangzhou, 510642, China; Guangdong Laboratory of Lingnan Modern Agriculture, Guangzhou, 510642, China; National Engineering Laboratory for Pollution Control and Waste Utilization in Livestock and Poultry Production, Changsha, Hunan 410125, China; Rutgers, the State University of New Jersey, New Brunswick, NJ 08901, USA. Electronic address:

Pennisetum sinese Roxb is a kind of forage with high yield and high quality. However, because only the leaves are used as feed, most straw is discarded or burned, causing pollution and resources waste. In this study, a magnetic cellulose adsorbent produced by extracting cellulose from Pennisetum sinese Roxb straw was used to adsorb antibiotic tetracycline (TC) from water and can be easily separated. The physicochemical properties of the obtained cellulose samples were studied. The adsorption process was mediated by multiple mechanisms including intra-particle diffusion, chemical ion exchange, hydrogen bonding, and electrostatic interaction. We determined the optimal pH, contact time, initial TC concentration, and temperature before investigating the effects of humic acid and ionic strength on the adsorption process. Our results demonstrate that the magnetic cellulose is a promising adsorbent for the removal of TC from water and is worth to be studied further to develop real-world implementation strategies.
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http://dx.doi.org/10.1016/j.carbpol.2020.117004DOI Listing
January 2021

Comparative Study on Chloride Binding Capacity of Cement-Fly Ash System and Cement-Ground Granulated Blast Furnace Slag System with Diethanol-Isopropanolamine.

Materials (Basel) 2020 Sep 16;13(18). Epub 2020 Sep 16.

China Electric Power Research Institute, Beijing 100192, China.

Steel bar corrosion caused by chloride was one of the main forms of concrete deterioration. The promotion of chloride binding capacity of cementitious materials would hinder the chloride transport to the surface of steel bar, thereby alleviating the corrosion and mitigating the deterioration. A comparative study on binding capacity of chloride in cement-fly ash system (C-FA) and cement-ground granulated blast furnace slag system (C-GGBS) with diethanol-isopropanolamine (DEIPA) was investigated in this study. Chloride ions was introduced by adding NaCl in paste, and the chloride binding capacity of the paste samples at 7 d and 60 d was examined. The hydration process was discussed via the testing of hydration heat and compressive strength. The hydrates in hardened paste was characterized by X-ray Diffractometry (XRD), Thermo Gravimetric Analysis (TGA), and Scanning Electron Microscope (SEM). The effect of DEIPA on dissolution of aluminate phase and compressive strength was discussed as well. These results showed that DEIPA could facilitate the hydration of C-FA and C-GGBS system, and the promotion effect was higher in C-FA than that in C-GGBS. DEIPA also increased the binding capacity of chloride in C-FA and C-GGBS systems. One reason was the increased chemical binding, because DEIPA facilitated the dissolution of aluminate to benefit the formation of Friedel's salt. Other reasons were the increased physical binding and migration resistance. By contrast, DEIPA presented greater ability to increase chloride binding capacity in C-FA system, because DEIPA showed stronger ability to expedite the dissolution of aluminate of FA than that of GGBS, which benefited the formation of FS, thereby promoting the chemical binding. Such results would give deep insight into using DEIPA as an additive in cement-based materials.
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http://dx.doi.org/10.3390/ma13184103DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7560300PMC
September 2020

Deep learning-based lumbosacral reconstruction for difficulty prediction of percutaneous endoscopic transforaminal discectomy at L5/S1 level: A retrospective cohort study.

Int J Surg 2020 Oct 1;82:162-169. Epub 2020 Sep 1.

Department of Orthopaedics, Shanghai Tenth People's Hospital, Tongji University School of Medicine, Shanghai, China; Spinal Pain Research Institute, Tongji University School of Medicine, Shanghai, China. Electronic address:

Background: Deep learning has been validated as a promising technique for automatic segmentation and rapid three-dimensional (3D) reconstruction of lumbosacral structures on CT. Simulated foraminoplasty of percutaneous endoscopic transforaminal discectomy (PETD) through the Kambin triangle may benefit viability assessment of PETD at L5/S1 level.

Material And Methods: Medical records and radiographic data of patients with L5/S1 lumbar disc herniation (LDH) who received a single-level PETD from March 2013 to February 2018 were retrospectively collected and analyzed. Deep learning was adopted to achieve semantic segmentation of lumbosacral structures (nerve, bone, disc) on CT, and the segmented masks on reconstructed 3D models. Two observers measured the area of the Kambin triangle on 6 selected deep learning-derived 3D (DL-3D) models and ground truth-derived 3D (GT-3D) models, and intraclass correlation coefficient (ICC) was calculated to assess the test-retest and interobserver reliability. Foraminoplasty of PETD was simulated on L5/S1 lumbosacral 3D models. Patients with extended foraminoplasty or stuck canula occurs on simulations were predicted as PETD-difficult cases (Group A). The remaining patients were regarded as PETD-normal cases (Group B). Clinical information and outcomes were compared between the two groups.

Results: Deep learning-derived 3D models of lumbosacral structures (nerves, bones, and disc) from thin-layer CT were reliable. The area of the Kambin triangle was 161.27 ± 40.10 mm on DL-3D models and 153.57 ± 32.37 mm on GT-3D models (p = 0.206). Reliability test revealed strong test-retest reliability (ICC between 0.947 and 0.971) and interobserver reliability of multiple measurements (ICC between 0.866 and 0.961). The average operation time was 99.62 ± 17.39 min in Group A and 88.93 ± 21.87 min in Group B (P = 0.025). No significant differences in patient-reported outcomes or complications were observed between the two groups (P > 0.05).

Conclusion: Deep learning achieved accurate and rapid segmentations of lumbosacral structures on CT, and deep learning-based 3D reconstructions were efficacious and reliable. Foraminoplasty simulation with deep learning-based lumbosacral reconstructions may benefit surgical difficulty prediction of PETD at L5/S1 level.
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http://dx.doi.org/10.1016/j.ijsu.2020.08.036DOI Listing
October 2020

Withdrawal of treatment in a pediatric intensive care unit at a Children's Hospital in China: a 10-year retrospective study.

BMC Med Ethics 2020 08 12;21(1):71. Epub 2020 Aug 12.

Department of Intensive Care Unit, Children's Hospital of Soochow University, No.92, Zhongnan street, Suzhou Industrial Park, Suzhou, Jiangsu, China.

Background: Published data and practice recommendations on end-of-life care generally reflect Western practice frameworks; there are limited data on withdrawal of treatment for children in China.

Methods: Withdrawal of treatment for children in the pediatric intensive care unit (PICU) of a regional children's hospital in eastern China from 2006 to 2017 was studied retrospectively. Withdrawal of treatment was categorized as medical withdrawal or premature withdrawal. The guardian's self-reported reasons for abandoning the child's treatment were recorded from 2011.

Results: The incidence of withdrawal of treatment for children in the PICU decreased significantly; for premature withdrawal the 3-year average of 15.1% in 2006-2008 decreased to 1.9% in 2015-2017 (87.4% reduction). The overall incidence of withdrawal of care reduced over the time period, and withdrawal of therapy by guardians was the main contributor to the overall reduction. The median age of children for whom treatment was withdrawn increased from 14.5 months (interquartile range: 4.0-72.0) in 2006 to 40.5 months (interquartile range: 8.0-99.0) in 2017. Among the reasons given by guardians of children whose treatment was withdrawn in 2011-2017, "illness is too severe" ranked first, accounting for 66.3%, followed by "condition has been improved" (20.9%). Only a few guardians ascribed treatment withdrawal to economic reasons.

Conclusions: The frequency of withdrawal of medical therapy has changed over time in this children's hospital PICU, and parental decision-making has been a large part of the change.
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http://dx.doi.org/10.1186/s12910-020-00517-yDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7425042PMC
August 2020

Energy and environmental impact assessment of a passive remediation bioreactor for antimony-rich mine drainage.

Environ Sci Pollut Res Int 2020 Oct 25;27(28):35040-35050. Epub 2020 Jun 25.

National-Regional Joint Engineering Research Center for Soil Pollution Control and Remediation in South China, Guangdong Key Laboratory of Integrated Agro-environmental Pollution Control and Management, Guangdong Institute of Eco-environmental Science & Technology, Guangdong Academy of Sciences, 808 Tianyuan Road, Guangzhou, 510650, Guangdong, China.

Industrial processes, such as smelting and mining, lead to antimony (Sb) contamination, which poses an environmental and human health risk. In this study, the energy consumption and environmental impacts of a passive biological treatment system were quantitatively evaluated using life cycle assessment (LCA), and the results were compared with that of an adsorption purification system. The results showed that the biosystem had a lower energy consumption compared with the adsorption system, with an energy savings of 27.39%. The environmental impacts of the bioreactor were also lower regarding acidification, ecotoxicity, carcinogens, climate change, resource depletion, and respiratory effects. The construction resulted in the most energy consumption (99%) for the passive bioreactor. Therefore, adopting environmentally friendly construction materials could make the biosystem a more energy-efficient option. Results demonstrated that the bioreactor in this research can have great potential for Sb mine drainage applications in terms of energy savings and environmental remediation without diminishing performance. The study findings can be useful for deciding the most energy effective process for mine drainage remediation. In addition, the identification of the energy and environmental impacts of the processes provide valuable information for the design of future systems that consume less materials and utilize new construction materials.
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http://dx.doi.org/10.1007/s11356-020-09816-8DOI Listing
October 2020

In-Frame and Frame-Shift Editing of the Gene to Develop Rice With Prolonged Basic Vegetative Growth Periods.

Front Plant Sci 2020 19;11:307. Epub 2020 Mar 19.

Fujian Key Laboratory of Genetic Engineering for Agriculture, Biotechnology Research Institute, Fujian Academy of Agricultural Sciences, Fuzhou, China.

rice has become increasingly popular in China owing to its superior grain quality. Over the past decades, " to " projects have been proposed to promote cultivation of rice in low latitudes in China. Traditionally, varieties were planted mainly in mid latitudes in the northeast plain and Yangtze River region. The key obstacle for introducing elite mid-latitude varieties to low latitudes is the severe shortening of growth period of the varieties due to their sensitivity to low-latitude short photoperiod and high temperature. Here we report development of new rice with prolonged basic vegetative growth (BVG) periods for low latitudes by targeted editing the () gene. Using CRISPR/Cas9 system, we generated both frame-shift and/or in-frame deletion mutants in four varieties, Nipponbare, Longdao16, Longdao24, and Xiushui134. When planting at low-latitude stations, the frame-shift homozygous lines exhibited significantly longer BVG periods compared with wild-types. Interestingly, we observed that minor deletion of the first few residues within the receiver domain could quantitatively impair the function of Ehd1 on activation of and , resulting in an intermediate-long BVG period phenotype in the homozygous in-frame deletion lines. Field investigation further showed that, both the in-frame and frame-shift lines exhibited significantly improved yield potential compared with wild-types. Our study demonstrates an effective approach to rapid breeding of elite varieties with intermediate-long and long BVG periods for flexible cropping systems in diverse areas or under different seasons in southern China, and other low-latitude regions.
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http://dx.doi.org/10.3389/fpls.2020.00307DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7096585PMC
March 2020

New insights for enhancing the performance of constructed wetlands at low temperatures.

Bioresour Technol 2020 Apr 3;301:122722. Epub 2020 Jan 3.

Shandong Key Laboratory of Water Pollution Control and Resource Reuse, School of Environmental Science and Engineering, Shandong University, Qingdao 266237, PR China.

Constructed wetlands (CWs) have been widely utilized for various types of wastewater treatment due to their merits, including high cost-effectiveness and easy operation. However, a few intrinsic drawbacks have always restricted their application and long-term stability, especially their weak performance at temperatures under 10 °C (low temperatures) due to the deterioration of microbial assimilation and plant uptake processes. The existing modifications to improve CWs performance from the direct optimization of internal components to the indirect adjunction of external resources promoted the wastewater treatment efficiency to a certain degree, but the sustainability and sufficiency of pollutants removal remains a challenge. With the goal of optimizing CW components, the integrity of the CW ecosystem and the removal of emerging pollutants, future directions for research should include radiation plant breeding, improvements to CW ecosystems, and the combination or integration of certain treatment processes with CWs to enhance wastewater treatment effects at low temperatures.
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http://dx.doi.org/10.1016/j.biortech.2019.122722DOI Listing
April 2020

Effect of Sodium Hexametaphosphate and Trisodium Phosphate on Dispersion of Polycarboxylate Superplasticizer.

Materials (Basel) 2019 Dec 13;12(24). Epub 2019 Dec 13.

China Electric Power Research Institute, Beijing 100192, China.

Enhancement in dispersion of polycarboxylate superplasticizer (PCE) could be obtained by incorporating retarders in normal concrete. The generally believed reason was that the consumption of free water and polymer at the beginning was reduced by retarding cement hydration. This theory could not convincingly explain why sodium hexametaphosphate (SHMP) was able to promote the dispersion capacity of PCE, while trisodium phosphate (TSP) could not, despite that both TSP and SHMP could obviously retard the cement hydration. The adsorption behavior of PCE and phosphate was investigated and the mechanism was analyzed in order to gain deeper understanding. The results showed that TSP and SHMP delayed the cement hydration, impeded adsorption process of PCE, and increased thickness of adsorption layer. It was interesting that TSP reduced the dispersion, but SHMP enhanced. The reason for this contradiction was due to the difference in composition of adsorption layer. In the PCE-TSP system, this layer was composed of the precipitates (formed by TSP and Ca) and the invalided PCE (caused by these precipitates in the immediate vicinity of the cement grains); the invalided PCE was due to the decrease of PCE dispersion. In the PCE-SHMP system, "Inner-phosphate (multi-layers) + Outer-PCE (single layer)" structure was formed to make the PCE work more effective, hence enhancing the dispersion. These results were expected to be useful for the design of highly efficient dispersants.
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http://dx.doi.org/10.3390/ma12244190DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6947408PMC
December 2019

Characteristics of human papillomaviruses distribution in Guizhou Province, China.

Virol J 2019 10 29;16(1):123. Epub 2019 Oct 29.

Department of Laboratory Medicine, Affiliated Hospital of Zunyi Medical University, Zunyi, Guizhou, People's Republic of China.

Background: Human papillomavirus (HPV) is one of the most common sexually transmitted viruses. Data about HPV infection in Guizhou is limited.

Methods: 56,768 cervical samples were collected and genotyped for 15 main high risk and 6 main low risk HPV types.

Results: 16.95% (9623/56768) of samples were HPV positive; 90.70% (8728/9623) of HPV positive women were infected by high risk HPV. High risk and high risk mix infection (1458; 70.85%) was the most common mix HPV infection type. The highest HPV detection rate was found in age group 41-45 years old (detection rate = 17.89%) (χ2 = 204.77; P < 0.001); the highest within-group HPV infection rates were found in the ≤20 (25.62%) and ≥ 61 (24.67%) years old age groups, the lowest within-group HPV infection rate was found in the 31-35 years old age group (15.02%). The highest mix infection proportions were found in the ≥61 (36.06%) and ≤ 20 (33.63%) years old age groups (χ2 = 111.21; P < 0.001), the lowest mix infection proportion was found in the 41-45 (17.42%) years old age group. The highest high risk infection proportions were found in the 26-30 (92.98%), ≥61 (92.68%), and 36-40 (92.16%) years old age groups (χ2 = 31.72; P < 0.001), the lowest high risk infection proportion was found in the ≤20 (84.96%) years old age group. HPV infection rates varied with seasons in Guizhou.

Conclusions: Characteristics of HPV distribution in Guizhou were identified. There were significant differences in HPV distribution among age groups, prevention strategies should be adjusted according to the characteristics.
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http://dx.doi.org/10.1186/s12985-019-1239-0DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6819633PMC
October 2019

The Association between Polymorphisms of Vitamin D Metabolic-Related Genes and Vitamin D Supplementation in Type 2 Diabetic Patients.

J Diabetes Res 2019 8;2019:8289741. Epub 2019 Sep 8.

Department of Occupational and Environmental Health, School of Public Health, Medical College of Soochow University, Suzhou, China.

Objective: To investigate the effect of single nucleotide polymorphisms (SNPs) of the key genes in vitamin D metabolic pathway on the serum 25(OH)D level after long-term vitamin D supplementation and provide a theoretical basis for rational vitamin D supplementation in diabetic patients with different genetic backgrounds.

Methods: Patients with type 2 diabetes (T2DM) who met the inclusive criteria were given 800 IU of vitamin D daily for 30 consecutive months. Serum 25(OH)D levels was measured at enrollment and every 6 months after enrollment. The average value of four-time measurements represented individual serum 25(OH)D level during vitamin D supplementation. Multiplex TaqMan genotyping was used to determine the distribution of eight candidate SNPs in genes of DHCR7, CYP2R1, CYP27B1, CYP24A1, and VDR, which are key genes in the vitamin D metabolic pathway, in diabetic patients.

Results: At baseline, the average serum 25(OH)D level was 22.71 ± 6.87 ng/mL, and 17.9% of patients had a ≥30 ng/mL level. During supplementation, the level of 25(OH)D increased significantly at each time point, and the average 25(OH)D level increased to 30.61 ± 5.04 ng/mL; however, there were 44.6% of patients whose serum 25(OH)D levels were still below 30 ng/mL. In the patients with CYP27B1 (rs10877012) G/T genotype, 71.79% achieved sufficient level of 25(OH)D, which was significantly higher than the other two genotypes ( < 0.05). Compared with those with T/T genotype, the RR of the patients with rs10877012 for <30 ng/mL level was 0.544 (95% CI: 0.291-0.917), and the RR after adjusting age and outdoor activity was 0.560 (95% CI: 0.292-0.970).

Conclusion: The serum 25(OH)D level in patients with diabetes mellitus after long-term vitamin D supplementation is associated with CYP27B1 polymorphism. Patients with rs10877012 G/T allele have a better response to vitamin D supplementation.

Trial Registration: This trial is registered with ChiCTR-IPC-17012657.
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http://dx.doi.org/10.1155/2019/8289741DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6754883PMC
February 2020

Genes Specifically Control Inner Floral Organ Morphogenesis and Pollen Development in Rice.

Plant Physiol 2019 12 23;181(4):1600-1614. Epub 2019 Sep 23.

Key Laboratory of Genetics, Breeding and Multiple Utilization of Crops, Ministry of Education and Fujian Provincial Key Laboratory of Crop Breeding by Design, Fujian Agriculture and Forestry University, Fuzhou 350002, China.

RETINOBLASTOMA-RELATED () is an essential gene in plants, but its molecular function outside of its role in cell cycle entry remains poorly understood. We characterized the functions of and in plant growth and development in rice using both forward- and reverse-genetics methods. The two genes were coexpressed and performed redundant roles in vegetative organs but exhibited separate functions in flowers. was highly expressed in the floral meristem and regulated the expression of floral homeotic genes to ensure floral organ formation. Mutation of caused loss of floral meristem identity, resulting in the replacement of lodicules, stamens, and the pistil with either a panicle-like structure or whorls of lemma-like organs. was preferentially expressed in stamens and promoted pollen formation. Mutation of led to deformed anthers without pollen. Similar to the protein interaction between AtRBR and AtMSI1 that is essential for floral development in Arabidopsis, OsMSI1 was identified as an interaction partner of OsRBR1 and OsRBR2. OsMSI1 was ubiquitously expressed and appears to be essential for development in rice (), as the mutation of was lethal. These results suggest that OsRBR1 and OsRBR2 function with OsMSI1 in reproductive development in rice. This work characterizes further functions of s and improves current understanding of specific regulatory pathways of floral specification and pollen formation in rice.
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http://dx.doi.org/10.1104/pp.19.00478DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6878013PMC
December 2019

Mixed Reality-Based Preoperative Planning for Training of Percutaneous Transforaminal Endoscopic Discectomy: A Feasibility Study.

World Neurosurg 2019 Sep 14;129:e767-e775. Epub 2019 Jun 14.

Orthopedic Department, Shanghai Tenth People's Hospital, Shanghai, China; Spinal Pain Research Institute, Tongji University School of Medicine, Shanghai, China. Electronic address:

Objective: To explore the effect of preoperative planning using mixed reality (MR) on training of percutaneous transforaminal endoscopic discectomy (PTED).

Methods: Before the training, we invited an experienced chief physician to plan the puncture path of PTED on the X-ray films of the lumbar spine model and the 3D Slicer platform, respectively, and used this as the standard to guide trainees. In the aggregate, 60 young residents were randomly divided into Group A (N = 30) and Group B (N = 30). Group A learned the 2-dimensional standard planning route, whereas Group B learned the standard route planning based on MR through the 3D Slicer platform. Then, trainees were asked to conduct PTED puncture on a lumbar spine model. Questionnaires were distributed to trainees before and after the training. During the training, puncture times, operating time (minutes), and fluoroscopy times were recorded.

Results: After the training, it was obvious that more trainees showed their recognition of MR, believing that MR could help preoperative planning and training of PTED. Their high satisfaction with the training indicated the success of our training. Moreover, puncture times, operating time (minutes), and fluoroscopy times of Group B were significantly lower than those of Group A.

Conclusions: MR technology contributes to preoperative planning of PTED and is beneficial in the training of PTED. It significantly reduces puncture times and fluoroscopy times, providing a standardized method for the training of PTED.
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http://dx.doi.org/10.1016/j.wneu.2019.06.020DOI Listing
September 2019

Novel OsGRAS19 mutant, D26, positively regulates grain shape in rice (Oryza sativa).

Funct Plant Biol 2019 08;46(9):857-868

Department of Bioengineering and Biotechnology, School of Chemical Engineering, Huaqiao University, Xiamen 361021, China; and Corresponding authors. Emails:

Grain size is an important factor in rice yield. Several genes related to grain size have been reported, but most of them are determined by quantitative trail loci (QTL) traits. Gene D26 is a novel site mutation of OsGRAS19 and involved in the brassinosteroid (BR) signalling pathway. However, whether D26 is involved in the process of rice reproductive development remains unclear. Here, gene cloning and functional analysis revealed that D26 has an obvious regulatory effect on grain size. Overexpression or CRISP/Cas9 mutant of D26 also showed that grain size was positively influenced. Cellular analyses show that D26 modulates grain size by promoting cell division and regulating the cell number in the upper epidermis of the glume. The overexpression results further suggest that the level of D26 expression positively impacts grain length and leaf angles and that the expression of several known grain size genes is involved in the regulation. Based on our results, D26, as a transcription factor, effectively improves rice grain shape.
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http://dx.doi.org/10.1071/FP18266DOI Listing
August 2019

Vitamin D Resists Cyclophosphamide-Induced Genomic and DNA Damage in CHL Cells In Vitro and in Mice In Vivo.

Nutr Cancer 2019 30;71(6):1030-1039. Epub 2019 Apr 30.

b School of Public Health , Soochow University , Suzhou , Jiangsu , People's Republic of China.

Vitamin D as an adjuvant therapy for cancer patients is hoped to have a beneficial outcome based on its physiological activity, but clinical trials so far by addition of vitamin D show unremarkable curative improvement, mechanism for explain this phenomena is not well-understood. The aim of this study was to determine whether vitamin D resists cyclophosphamide (CP)-induced genomic and DNA damage. In CHL cells in vitro, 1α,25-(OH)D at 10, 50, and 100 nM was found to alleviate the frequency of chromosomal aberration with an alleviation range of 40.7-44.0%. There was a dose-dependent decrease for a proportion of γ-H2AX foci positive cells in response to an increase in 1α,25-(OH)D concentration. Two vitamin D injections of 1,000, 5,000, or 10,000 IU suppressed CP-induced micronucleus formation in mice BMCs with an alleviation range of 36.7-44.5%, mitigated lymphocytes DNA damage reflected by lower tail DNA, tail length and olive tail moment parameter in comet assay. Vitamin D showed an antagonistic effect on CP-induced genomic and DNA damage. Our data suggest that vitamin D as an adjuvant combine antineoplastic drug with genotoxicity administer to tumor patients is contraindicant.
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http://dx.doi.org/10.1080/01635581.2019.1595050DOI Listing
June 2020

CRISPR/Cas9-mediated functional recovery of the recessive rc allele to develop red rice.

Plant Biotechnol J 2019 11 7;17(11):2096-2105. Epub 2019 May 7.

Biotechnology Research Institute, Fujian Academy of Agricultural Sciences, Fuzhou, China.

Red rice contains high levels of proanthocyanidins and anthocyanins, which have been recognized as health-promoting nutrients. The red coloration of rice grains is controlled by two complementary genes, Rc and Rd. The RcRd genotype produces red pericarp in wild species Oryza rufipogon, whereas most cultivated rice varieties produce white grains resulted from a 14-bp frame-shift deletion in the seventh exon of the Rc gene. In the present study, we developed a CRISPR/Cas9-mediated method to functionally restore the recessive rc allele through reverting the 14-bp frame-shift deletion to in-frame mutations in which the deletions were in multiples of three bases, and successfully converted three elite white pericarp rice varieties into red ones. Rice seeds from T in-frame Rc lines were measured for proanthocyanidins and anthocyanidins, and high accumulation levels of proanthocyanidins and anthocyanidins were observed in red grains from the mutants. Moreover, there was no significant difference between wild-type and in-frame Rc mutants in major agronomic traits, indicating that restoration of Rc function had no negative effect on important agronomic traits in rice. Given that most white pericarp rice varieties are resulted from the 14-bp deletion in Rc, it is conceivable that our method could be applied to most white pericarp rice varieties and would greatly accelerate the breeding of new red rice varieties with elite agronomic traits. In addition, our study demonstrates an effective approach to restore recessive frame-shift alleles for crop improvement.
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http://dx.doi.org/10.1111/pbi.13125DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6790373PMC
November 2019

Maternal dietary patterns, supplements intake and autism spectrum disorders: A preliminary case-control study.

Medicine (Baltimore) 2018 Dec;97(52):e13902

Department of Psychiatry & Mental Health Institute of the Second Xiangya Hospital, Central South University, the China National Clinical Research Center for Mental Health Disorders, National Technology Institute of Psychiatry, Key Laboratory of Psychiatry and Mental Health of Hunan Province.

The aim of this study was first to investigate associations between maternal dietary patterns and autism spectrum disorders (ASDs) and second to investigate association between maternal supplement intake and ASD.We used a case-control study design to enroll typically developing (TD) children and children with ASD, and data were derived from the Autism Clinical and Environmental Database (ACED).Three seventy four children with AUTISM and 354 age matched TD children were included. The multivariate logistic regression model revealed that maternal unbalanced dietary patterns before conception had a significant increased risk of ASD in offspring (mostly meat: adjusted OR, 4.010 [95% CI, 1.080, 14.887]; mostly vegetable: adjusted OR, 2.234 [95% CI, 1.009, 4.946]); maternal supplementation of calcium during pregnancy preparation was associated with decreased ASD risk (adjusted OR, 0.480 [95% CI, 0.276, 0.836]).This study provided preliminary evidence that maternal unbalanced dietary patterns may be a risk factor for ASD and supplementation of calcium during pregnancy preparation may be inversely associated with ASD in offspring.
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http://dx.doi.org/10.1097/MD.0000000000013902DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6314746PMC
December 2018

Neo-5,22-Cholestadienol Derivatives from Karsch and Targeted Bactericidal Action Mechanisms.

Molecules 2018 Dec 26;24(1). Epub 2018 Dec 26.

Life Science and Environmental Science Research Center of Harbin University of Commerce, Harbin 150025, China.

The discovery and search for new antimicrobial molecules from insects and animals that live in polluted environments is a very important step in the scientific search for solutions to the current problem of antibiotic resistance. Previously, we have reported that the secondary metabolite with the antibacterial action discovered in scorpion. The current study further isolated three new compounds from karsch, while compounds and possessed 5,22-cholestadienol derivatives whose structure demonstrated broad spectrum bactericide activities. To explore the antibacterial properties of these new compounds, the result shows that compound inhibited bacterial growth of both and in a bactericidal rather than a bacteriostatic manner (MBC/MIC ratio ≤ 2). Similarly, with compound , a ratio of MBC/MIC ≤ 2 indicates bactericidal activity inhibited bacterial growth of Remarkably, this suggests that two compounds can be classified as bactericidal agents against broad spectrum bactericide activities for 5,22-cholestadienol derivatives from karsch. The structures of compounds ⁻ were established by comprehensive spectra analysis including two-dimensional nuclear magnetic resonance (2D-NMR) and high-resolution electrospray ionization-mass spectrometry (HRESI-MS) spectra. The antibacterial mechanism is the specific binding (various of bonding forces between molecules) using compound or as a ligand based on the different receptor proteins'-2XRL or 1Q23-active sites from bacterial ribosome unit A, and thus prevent the synthesis of bacterial proteins. This unique mechanism avoids the cross-resistance issues of other antibacterial drugs.
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http://dx.doi.org/10.3390/molecules24010072DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6337218PMC
December 2018

Acute effect of soy and soy products on serum uric acid concentration among healthy Chinese men.

Asia Pac J Clin Nutr 2018;27(6):1239-1242

School of Public Health, Bengbu Medical College, Anhui, China.

Background And Objectives: Soy products are essential to the daily life of the Chinese population. However, the association between soy products and serum uric acid remains unclear. Better understanding of their relationship could provide food choice information for patients with gout. This study assessed the acute effects of soy and soy products on serum uric acid.

Methods And Study Design: Sixty healthy adult male volunteers were recruited and randomly assigned to six groups. Ten participants in each group randomly ingested one of six foods: water, soy, and four different soy products. A blood test was conducted after 3 h to examine uric acid concentration.

Results: The serum uric acid concentration significantly increased by 21.4±23.4 μmol/L at 1 h and 16.3±19.4 μmol/L at 2 h following ingestion of whole soybeans. These changes also applied to the soy powder group. The serum uric acid concentration rapidly increased by 38.1±20.5 μmol/L at 1 h, 34.4±18.2 μmol/L at 2 h, and 24.1±24.2 μmol/L at 3 h after the ingestion of soybean milk. The maximum concentration of serum uric acid was observed at 1 h after intake of soybeans and soy products, and then gradually decreased during the subsequent 2-h period. No significant uric acid changes from ingesting bean curd cake and dried bean curd stick were detected.

Conclusions: Ingesting different soy products resulted in different concentrations of serum uric acid, with soybeans, soybean milk, and soy powder considerably increasing serum uric acid.
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http://dx.doi.org/10.6133/apjcn.201811_27(6).0010DOI Listing
September 2019

miR-339-5p inhibits lung adenocarcinoma invasion and migration by directly targeting BCL6.

Oncol Lett 2018 Nov 31;16(5):5785-5790. Epub 2018 Aug 31.

The Second Department of Respiratory Medicine, People's Hospital of Linyi, Linyi, Shandong 276003, P.R. China.

Lung adenocarcinoma (LA) is a common non-small cell lung cancer, but effective biomarkers to diagnose early LA are still lacking. Increasing evidence has indicated that the dysregulation of microRNAs (miRNAs) play crucial roles in LA progression. miR-339-5p has been recently confirmed to exert crucial functions in various cancers. Nevertheless, molecular mechanisms and effects of miR-339-5p on LA development still remain vague. In the present research, miR-339-5p expression was downregulated in human LA tissues. miR-339-5p overexpression in LA cells could remarkably suppress the LA cell invasion and migration. In addition, further studies indicated that miR-339-5p overexpression downregulated both the B-cell lymphoma 6 (BCL6) mRNA and protein expressions by targeting the BCL6 3'-UTR directly. Moreover, BCL6 knockdown could partially lessen the function of miR-339-5p in LA invasion and migration. In conclusion, the present data identified miR-339-5p as a novel LA suppressor which exerted its functions partly by negatively regulating BCL6.
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http://dx.doi.org/10.3892/ol.2018.9376DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6176402PMC
November 2018

Microbial nitrogen removal of ammonia wastewater in poly (butylenes succinate)-based constructed wetland: effect of dissolved oxygen.

Appl Microbiol Biotechnol 2018 Nov 27;102(21):9389-9398. Epub 2018 Sep 27.

Shandong Key Laboratory of Water Pollution Control and Resource Reuse, School of Environmental Science & Engineering, Shandong University, Jinan, 250100, China.

Constructed wetland (CW) is popular in wastewater treatment for its prominent advantage of low construction and operation cost. However, the nitrogen removal in conventional CW is usually limited by the low dissolved oxygen (DO) and insufficient electron donor. This paper investigated the nitrogen removal performance and mechanisms in the poly (butylenes succinate)-based CW (PBS-CW) while treating ammonia wastewater under different DO levels. The average DO contents in limited-aeration and full-aeration phases were 1.68 mg L and 5.71 mg L, respectively. Results indicated that, with the ammonia nitrogen loading rate of 25 g N m day, total nitrogen removal ratios in the PBS-CW under the limited-aeration and full-aeration phases were 72% and 99%, respectively. Combined analyses revealed that simultaneous nitrification and denitrification (SND) via nitrite/nitrate were the main microbial nitrogen removal pathways in the aeration phase of the PBS-CW (> 89%). The microbial carrier of biodegradable material was believed to play a significant role in prompting SND performance while dealing with low C/N wastewater. Due to the coexistence of micro-anaerobic zone and carbon supply inside the coated biofilm, the high DO level in the PBS-CW increased the abundance of the nitrifying bacteria (amoA and nxrA), denitrifying bacteria (narG, nirK, nirS, and nosZ), and even anammox bacteria (anammox 16s rRNA). These features are beneficial to many microbial processes which require the simultaneous aerobic, anoxic, and anaerobic environment.
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http://dx.doi.org/10.1007/s00253-018-9386-6DOI Listing
November 2018

Effects of levocarnitine on cardiac function, urinary albumin, hs-CRP, BNP, and troponin in patients with coronary heart disease and heart failure.

Hellenic J Cardiol 2020 Mar - Apr;61(2):99-102. Epub 2018 Sep 6.

Emergent Intensive Care Unit, The First People's Hospital of Jining, Jining 272011, PR China. Electronic address:

Objective: To investigate the effects of levocarnitine on cardiac function, urinary albumin (ALB), high-sensitivity C-reactive protein (hs-CRP), brain natriuretic peptide (BNP), and troponin in patients with coronary heart disease (CHD) and heart failure (HF).

Methods: In total, 246 patients with CHD-caused HF were selected and randomly divided into Group A and Group B. A fully automatic biochemical analyzer was used to measure the levels of ALB, hs-CRP, BNP, and troponin in both groups of patients, and the expression levels of LVDD and LVEF were detected by cardiac color ultrasonography. Patients in Group B were intravenously injected with 3.0 g of levocarnitine, once per day. After 14 days, changes in levels of ALB, hs-CRP, BNP, troponin, LVDD, and LVEF in Group A patients were detected.

Results: The effective cure rates of patients in both groups were 65.8% and 81.3%, respectively, and there was a statistically significant difference between the two groups (p < 0.05). After administration of levocarnitine, all indicators showed decreasing trends, but the LVEF level increased. Among them, patients treated with levocarnitine showed the most evident decrease in LVEF. Decrease in BNP was the largest (p < 0.05). Additionally, there was no statistical difference in incidence rate between the two groups (5.8% vs. 2.5%, p = 0.222).

Conclusion: Levocarnitine can effectively improve ALB, hs-CRP, BNP, troponin, and LVDD levels to improve cardiac function rating and thus improve cardiac function.
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http://dx.doi.org/10.1016/j.hjc.2018.08.006DOI Listing
September 2018

Effects of transplanted adipose derived stem cells on the expressions of α-SMA and DCN in fibroblasts of hypertrophic scar tissues in rabbit ears.

Exp Ther Med 2018 Sep 29;16(3):1729-1734. Epub 2018 Jun 29.

Health Ward, People's Hospital of Zhangqiu District, Jinan, Shandong 250200, P.R. China.

To study the effects of transplanted adipose derived stem cells (ADSCs) on the expressions of α-smooth muscle actin (α-SMA) and decorin (DCN) in fibroblasts of hypertrophic scar tissues in rabbit ears. Twelve New Zealand white rabbits were selected; the normal subcutaneous adipose tissues in inguinal region were removed, ADSCs were extracted via enzyme digestion, cultured in Dulbecco's modified Eagle's medium (DMEM) and inoculated into the culture dish (3-5×10 cells/ml). After the rabbit ear hypertrophic scar model was established successfully, the fibroblasts of hypertrophic scar tissues in rabbit ears were separated and cultured using the mechanical method combined with enzyme digestion, and the ADSCs and scar fibroblasts were cultured in non-contact Transwell co-culture system for 21 days (experimental group); the corresponding scar fibroblasts were cultured in an ordinary 6-well plate without any treatment for 21 days (control group). The content of collagen I in fibroblasts was detected using the enzyme-linked immunosorbent assay (ELISA) kit, the mRNA expressions of α-SMA and DCN were detected via reverse transcription-polymerase chain reaction (RT-PCR), the protein expressions of α-SMA and DCN were detected via western blot analysis, and the expressions and distribution of α-SMA and DCN were detected via immunofluorescence assay. The results of ELISA showed that the content of collagen I in experimental group was decreased significantly (p<0.01). The results of RT-PCR and western blot analysis revealed that the mRNA and protein expression levels of α-SMA were significantly decreased (P<0.01, but those of DCN were significantly increased (p<0.01). Moreover, the results of immunofluorescence assay showed that the expression of α-SMA in experimental group was significantly decreased, while the expression of DCN was significantly increased. ADSCs can inhibit the mRNA and protein expressions of α-SMA and promote the mRNA and protein expressions of DCN in culture system, and they are expected to be used in the prevention and treatment of pathological scars.
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http://dx.doi.org/10.3892/etm.2018.6383DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6122172PMC
September 2018

Aberrant GlyRS-HDAC6 interaction linked to axonal transport deficits in Charcot-Marie-Tooth neuropathy.

Nat Commun 2018 03 8;9(1):1007. Epub 2018 Mar 8.

Department of Molecular Medicine, The Scripps Research Institute, La Jolla, CA, 92037, USA.

Dominant mutations in glycyl-tRNA synthetase (GlyRS) cause a subtype of Charcot-Marie-Tooth neuropathy (CMT2D). Although previous studies have shown that GlyRS mutants aberrantly interact with Nrp1, giving insight into the disease's specific effects on motor neurons, these cannot explain length-dependent axonal degeneration. Here, we report that GlyRS mutants interact aberrantly with HDAC6 and stimulate its deacetylase activity on α-tubulin. A decrease in α-tubulin acetylation and deficits in axonal transport are observed in mice peripheral nerves prior to disease onset. An HDAC6 inhibitor used to restore α-tubulin acetylation rescues axonal transport deficits and improves motor functions of CMT2D mice. These results link the aberrant GlyRS-HDAC6 interaction to CMT2D pathology and suggest HDAC6 as an effective therapeutic target. Moreover, the HDAC6 interaction differs from Nrp1 interaction among GlyRS mutants and correlates with divergent clinical presentations, indicating the existence of multiple and different mechanisms in CMT2D.
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http://dx.doi.org/10.1038/s41467-018-03461-zDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5843656PMC
March 2018

Elevated Cathepsin K potentiates metastasis of epithelial ovarian cancer.

Histol Histopathol 2018 Jul 5;33(7):673-680. Epub 2018 Jan 5.

The Department of Obstetrics, People's Hospital of Weifang, Weifang, Shandong, P.R. China.

Cathepsin K, or CTSK, has been found to be involved in the peritoneal metastasis of ovarian carcinoma. However, the expression and clinicopathological significance of CTSK remains unknown in epithelial ovarian cancer (EOC). The aim of the present study was to investigate the expression of CTSK and its clinicopathological significance in EOC. CTSK expression was evaluated using immunohistochemistry in EOC tissue microarray. The expression of CTSK in EOC was displayed to be markedly higher than that of adjacent normal control. In addition, CSTK expression was shown to be remarkably associated with metastases and inferior overall prognosis of EOC. In vitro, Knock-down of CTSD was exhibited to be able to suppress migration and invasion in EOC cell lines OV-2008 but not proliferation in OV-2008. Together, our data showed that elevated CTSD in EOC can potentiate the metastasis of EOC cells, suggesting that targeting CTSD might be used as a novel therapeutic target for EOC.
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http://dx.doi.org/10.14670/HH-11-960DOI Listing
July 2018