Publications by authors named "Huanhuan Li"

347 Publications

Successful treatment of fulminant myocarditis with intra-aortic balloon pump counterpulsation combined with immunoglobulin and glucocorticoid in a young male adult.

Authors:
Huanhuan Li Lun Li

Front Cardiovasc Med 2022 22;9:905189. Epub 2022 Jul 22.

Department of Cardiology, Wuhan Fourth Hospital, Wuhan, China.

Background: Fulminant myocarditis (FM) is a serious non-specific inflammatory disease of the myocardium. FM tends to occur in adolescents and the course of the disease progresses rapidly. It is prone to cardiogenic shock (CGS) and multiple organ failure (MOF) with high mortality. We report a case of FM with CGS and MOF in a young male who was successfully treated with intra-aortic balloon pump counterpulsation (IABP) combined with intravenous immunoglobulin (IVIG) and glucocorticoid (GC).

Case Summary: A 21-year-old previously healthy man presented with fever, headache, and chest tightness. He came to the hospital for emergency treatment. The laboratory data showed that the levels of serum cardiac troponin I (cTnI), N-terminal B-type natriuretic peptide (NT-proBNP), myocardial zymogram, and neutrophils increased. Echocardiography showed pericardial effusion and decreased left ventricular systolic function. ECG showed diffuse ST-segment elevation. He was clinically diagnosed with FM and admitted to the intensive care unit for treatment. Within 48 h of admission, the clinical course of the patient deteriorated rapidly, with CGS accompanied by MOF, high atrioventricular block (AVB), and ventricular tachycardia (VT). After using mechanical circulatory support (MCS) therapy with IABP, IVIG, GC, continuous renal replacement therapy (CRRT), and mechanical ventilation complicated with a temporary cardiac pacemaker, he recovered normal cardiac function. He made a full recovery and was discharged home on day 21.

Discussion: For patients with FM, early diagnosis, close monitoring, timely use of MCS devices, and active comprehensive treatment are very important. MCS devices such as IABP can become lifesaving tools for the treatment of FM.
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http://dx.doi.org/10.3389/fcvm.2022.905189DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9353579PMC
July 2022

Intelligent evaluation of free amino acid and crude protein content in raw peanut seed kernels using NIR spectroscopy paired with multivariable calibration.

Anal Methods 2022 Aug 2. Epub 2022 Aug 2.

School of Food and Biological Engineering, Jiangsu University, Zhenjiang, 212013, P. R. China.

Given the nutritional importance of peanuts, this study examined the free amino acid (FAA) and crude protein (CP) content in raw peanut seeds. Near-infrared spectroscopy (NIRS) was employed in combination with variable selection algorithms after successful reference data analysis using colorimetric and Kjeldahl methods. Ensuing the application of partial least squares (PLS) as a full spectral model, the genetic algorithm (GA), bootstrapping soft shrinkage (BOSS), uninformative variable elimination (UVE), and random frog (RF) models were tested and assessed. A comparison of correlation coefficients of prediction (), root mean square error of prediction (RMSEP), and residual predictive deviation (RPD) was performed to appraise the performance of the built models. Using RF-PLS, an unsurpassed outcome was achieved for FAA ( = 0.937, RPD = 3.38) and CP ( = 0.9261, RPD = 3.66). These findings demonstrated that NIR in combination with RF-PLS could be utilized for quantitative, rapid, and nondestructive prediction of FAA and CP in raw peanut seed samples.
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http://dx.doi.org/10.1039/d2ay00875kDOI Listing
August 2022

Simultaneous determination of benzimidazole fungicides in food using signal optimized label-free HAu/Ag NS-SERS sensor.

Food Chem 2022 Jul 20;397:133755. Epub 2022 Jul 20.

College of Food and Biological Engineering, Jimei University, Xiamen 361021, PR China; School of Food and Biological Engineering, Jiangsu University, Zhenjiang 213013, PR China. Electronic address:

Extensively employed pesticide in agriculture causes residue in food products that would threaten public health safety. The surface-enhanced Raman scattering (SERS) signal reliant on double sensing of carbendazim and thiabendazole in a single step is achieved without the aid of any bio-recognition element. A label-free anisotropic bimetallic hollow Au/Ag nanostars (HAu/Ag NS) SERS substrate was synthesized with numerous hot spots for Raman molecule through a galvanic displacement-free deposition. The individual and mixed analyte calibration results were compared based on the identified peak at 1224 (carbendazim) and 778 (thiabendazole) cm and exhibited insignificant differences. The sensor could detect carbendazim and thiabendazole up to 4.28 × 10 and 6.04 × 10 µg·g or µg·mL in both individual and mixture of their extract. The recovery for accuracy and precision analysis was 91.54-98.26 % in rice and water. Finally, validation results were achieved satisfactorily (p > 0.05) with HPLC.
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http://dx.doi.org/10.1016/j.foodchem.2022.133755DOI Listing
July 2022

Lithiophilic Sn-Co nano-seeds sealed in a hollow carbon shell to stabilize lithium metal anodes.

Chem Commun (Camb) 2022 Jul 27. Epub 2022 Jul 27.

Hunan Provincial Key Laboratory of Chemical Power Sources, College of Chemistry and Chemical Engineering, Central South University, Changsha, 410083, P. R. China.

A lithiophilic Sn-Co nano-seed sealed in a nitrogen-doped carbon shell is designed to stabilize lithium metal anodes, in which lithiophilic alloys can regulate lithium deposition behavior and the hollow carbon shell is beneficial to prevent agglomeration. The modified lithium anode can be stable for 1350 h and 400 h under 1 mA cm and 5 mA cm in symmetric cells. The [email protected]@Li||LiFePO full cell with a low N/P ratio of 2.12 shows a superior capacity retention of >98% over 250 cycles under 1C.
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http://dx.doi.org/10.1039/d2cc02932dDOI Listing
July 2022

Lithiophilic Sn-Co nano-seeds sealed in a hollow carbon shell to stabilize lithium metal anodes.

Chem Commun (Camb) 2022 Jul 27. Epub 2022 Jul 27.

Hunan Provincial Key Laboratory of Chemical Power Sources, College of Chemistry and Chemical Engineering, Central South University, Changsha, 410083, P. R. China.

A lithiophilic Sn-Co nano-seed sealed in a nitrogen-doped carbon shell is designed to stabilize lithium metal anodes, in which lithiophilic alloys can regulate lithium deposition behavior and the hollow carbon shell is beneficial to prevent agglomeration. The modified lithium anode can be stable for 1350 h and 400 h under 1 mA cm and 5 mA cm in symmetric cells. The [email protected]@Li||LiFePO full cell with a low N/P ratio of 2.12 shows a superior capacity retention of >98% over 250 cycles under 1C.
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http://dx.doi.org/10.1039/d2cc02932dDOI Listing
July 2022

Development and Validation of a Nomogram for Predicting Generalization in Patients With Ocular Myasthenia Gravis.

Front Immunol 2022 7;13:895007. Epub 2022 Jul 7.

Department of Neurology, Tangdu Hospital, The Fourth Military Medical University, Xi'an, China.

Background: This study aims to develop and validate a nomogram for predicting 1- and 2-year generalization probabilities in patients with ocular myasthenia gravis (OMG).

Methods: In total, 501 eligible patients with OMG treated at seven tertiary hospitals in China between January 2015 and May 2019 were included. The primary outcome measure was disease generalization. A nomogram for predicting 1- and 2-year generalization probabilities was constructed using a stepwise Cox regression model. Nomogram performance was quantified using C-indexes and calibration curves. Two-year cumulative generalization rates were analyzed using the Kaplan-Meier method for distinct nomogram-stratified risk groups. The clinical usefulness of the nomogram was evaluated using decision curve analysis (DCA).

Result: The eligible patients were randomly divided into a development cohort (n=351, 70%) and a validation cohort (n=150, 30%). The final model included five variables: sex, onset age, repetitive nerve stimulation findings, acetylcholine receptor antibody test results, and thymic status. The model demonstrated good discrimination (C-indexes of 0.733 and 0.788 in the development and validation cohorts, respectively) and calibration, with good agreement between actual and nomogram-estimated generalization probabilities. Kaplan-Meier curves revealed higher 2-year cumulative generalization rates in the high-risk group than that in the low-risk group. DCA demonstrated a higher net benefit of nomogram-assisted decisions compared to treatment of all patients or none.

Conclusion: The nomogram model can predict 1- and 2-year generalization probabilities in patients with OMG and stratified these patients into distinct generalization risk groups. The nomogram has potential to aid neurologists in selecting suitable patients for initiating immunotherapy and for enrolment in clinical trials of risk-modifying treatments.
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http://dx.doi.org/10.3389/fimmu.2022.895007DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9302474PMC
July 2022

Prophylactic extended-field irradiation for locally advanced cervical cancer.

Gynecol Oncol 2022 Jul 19. Epub 2022 Jul 19.

Department of radiation oncology, The Second affiliated Hospital of Jilin University, Changchun 130041, China. Electronic address:

Concomitant chemoradiotherapy is the standard treatment for locally advanced cervical cancer. Pelvic irradiation is commonly recommended for patients with negative para-aortic lymph nodes(PALNs). However, owing to the development of imaging-guided brachytherapy, distant failure has become the main failure pattern. The PALNs are a vital site of distant metastasis, and the para-aortic region may contain occult microscopic metastases that are barely detected owing to imaging technology restriction. The prognostic of patients who experienced PALN failure is dismal. Typically, there are four ways to decrease PALN failure. First, surgical staging can be performed to assess the occurrence of metastasis in the para-aortic region; however, the application of surgical staging is decreasing owing to controversial survival benefits and accompanying complications of surgery. Second, regular imaging surveillance and timely salvage of early recurrences could reduce PALN failure. Third, better systemic adjuvant therapy could be recommended since it has enormous potential to reduce distant metastases and improve overall survival. Fourth, performing prophylactic extended-field irradiation (EFI), including pelvic and para-aortic region irradiation, can sterilize occult microscopic metastases in the para-aortic region and improve survival. Prior investigations have revealed that prophylactic EFI could reduce PALN failure as well as distant metastasis and present the benefit of survival. Yet, owing to the serious morbidity induced by enlarged irradiation field in the era of conventional irradiation techniques, further research on EFI is stagnated. Nowadays, with the development of new technologies, intensity modulated radiation therapy can deliver a higher dose to tumors with acceptable toxicity. Prophylactic EFI regained attention. However, the inclusion criteria of prophylactic EFI in existing studies reveal great discrepancies. Thus, it is urgent to precisely identify indications for better survival and lower complications in patients with cervical cancer. In this review, we identify indications and summary guidelines for prophylactic EFI, which may provide a foundation for further trials and clinical applications.
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http://dx.doi.org/10.1016/j.ygyno.2022.07.009DOI Listing
July 2022

A target-responsive release SERS sensor for sensitive detection of tetracycline using aptamer-gated HP-UiO-66-NH nanochannel strategy.

Anal Chim Acta 2022 Aug 2;1220:339999. Epub 2022 Jun 2.

School of Food and Biological Engineering, Jiangsu University, Zhenjiang, 212013, PR China. Electronic address:

In this study, a target-responsive release Surface-enhanced Raman scattering (SERS) sensor was developed for sensitive detection of tetracycline (TTC) using aptamer-gated HP-UiO-66-NH nanochannel strategy. The hierarchically porous zirconium-based metal-organic frameworks (HP-UiO-66-NH MOFs) with high surface areas and the conjugated π-electron system were first synthesized and served as the nanocontainers. The methylene blue (MB) and gold nanoparticles (AuNPs) were then loaded in the pores of HP-UiO-66-NH as the signal probes and capping agent, respectively. Thereafter, aptamer was assembled on the surface of HP-UiO-66-NH based on the π-π stacking interaction. In the presence of TTC, the aptamer "molecular gate" was opened, resulting in the "cargo release" of MB and AuNPs. Hence, the amount of TTC could be determined by monitoring the change of SERS intensity of the supernatant. Under the optimal conditions, a good linear correlation between SERS intensity (886 cm) and TTC concentration was observed with the concentration from 0.01 to 10000 ng/mL, resulting in a relatively low detection limit of 0.01 ng/mL. Moreover, this method showed a promising practical application in spiked real samples (milk and pork) with recoveries of 93.23-108.79%, which confirmed its great potential in antibiotic detection.
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http://dx.doi.org/10.1016/j.aca.2022.339999DOI Listing
August 2022

Recombinant jurkat cells (HMGN2-T cells) secrete cytokines and inhibit the growth of tumor cells.

J Mol Histol 2022 Jul 21. Epub 2022 Jul 21.

Department of Hematology, Peking University First Hospital, No.8 Xishiku Street, Xicheng District, 100034, Beijing, China.

High Mobility Group Chromosomal Protein N2 (HMGN2) can recognize tumor cells and enhance the anti-tumor effect of immune cells. This study aimed to establish a lentiviral vector of recombinant HMGN2 gene, establish recombinant T cells (HMGN2-T cells), and observe their anti-tumor effects. Total RNA was isolated from peripheral blood mononuclear cells. HMGN2, cluster of differentiation (CD) 8 A, CD28, CD137, and CD3ζ genes were amplified and connected. Jurkat cells were transfected with the recombinant lentivirus vector. The viability, apoptosis, and cell cycle of HMGN2-T cells were detected using Cell Counting Kit-8 assay and flow cytometry. The co-culture was performed by adding HMGN2-T cells to tumor cells with different effect-to-target (E:T) ratios. The cytotoxic activity was measured by lactate dehydrogenase (LDH) releasing assay. The sequences of HMGN2, CD8A, CD28, CD137, and CD3ζ gene plasmids were confirmed using gene sequencing. After the lentiviral transfection for 72 h, green fluorescence cells (HMGN2-T cells) could be seen. Cell viability and apoptosis were increased in HMGN2-T cells. The cytokine levels of interleukin 2 (IL-2) and tumor necrosis factor α (TNF-α) increased in cell supernatants of HMGN2-T cells. The percentage of G0/G1 phase cells was lower, the rate of S phase cells was higher in HMGN2-T cells than control cells. The co-culture of HMGN2-T cells and tumor cells could promote the cytokines' release. The LDH level was increased with the elevation of E:T ratios. In conclusion, the HMGN2-T cells were well-established and have the effect of secreting cytokines and killing tumor cells.
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http://dx.doi.org/10.1007/s10735-022-10084-8DOI Listing
July 2022

Mapping of the novel powdery mildew resistance gene Pm2Mb from Aegilops biuncialis based on ph1b-induced homoeologous recombination.

Theor Appl Genet 2022 Jul 13. Epub 2022 Jul 13.

The State Key Laboratory of Wheat and Maize Crop Science, College of Life Sciences, Henan Agricultural University, Zhengzhou, 450002, Henan, China.

Key Message: A novel powdery mildew resistance gene Pm2Mb from Aegilops biuncialis was transferred into common wheat and mapped to chromosome 2ML bin FL 0.49-0.66 by molecular cytogenetic analysis of 2M recombinants. Aegilops biuncialis, a wild relative of common wheat, is highly resistant to powdery mildew. Previous studies identified that chromosome 2M in Chinese Spring (CS)-Ae. biuncialis 2M disomic addition line TA7733 conferred high resistance to powdery mildew, and the resistance gene was temporarily designated as Pm2Mb. In this study, a total of 65 CS-Ae. biuncialis 2M recombinants were developed by ph1b-induced homoeologous recombination and they were grouped into 12 different types based on the presence of different markers of 2M-specificity. Segment sizes and breakpoints of each 2M recombinant type were further characterized using in situ hybridization and molecular marker analyses. Powdery mildew responses of each type were assessed by inoculation of each 2M recombinant-derived F progenies using the isolate E05. Combined analyses of in situ hybridization, molecular markers and powdery mildew resistance data of the 2M recombinants, the gene Pm2Mb was cytologically located to an interval of FL 0.49-0.66 in the long arm of 2M, where 19 2M-specific markers were located. Among the 65 2M recombinants, T-11 (T2DS.2DL-2ML) and T-12 (Ti2DS.2DL-2ML-2DL) contained a small 2ML segment harboring Pm2Mb. Besides, a physical map of chromosome 2M was constructed with 70 2M-specific markers in 10 chromosomal bins and the map showed that submetacentric chromosome 2M of Ae. biuncialis was rearranged by a terminal intrachromosomal translocation. The newly developed 2M recombinants with powdery mildew resistance, the 2M-specific molecular markers and the physical map of chromosome 2M will benefit wheat disease breeding as well as fine mapping and cloning of Pm2Mb.
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http://dx.doi.org/10.1007/s00122-022-04162-4DOI Listing
July 2022

Experimental and Simulation Study for the Influence of Thermal Pre-Deformation on Subsequent Aging Precipitation Kinetics of Al-Zn-Mg-Cu Alloy.

Materials (Basel) 2022 Jul 1;15(13). Epub 2022 Jul 1.

School of Automotive Engineering, Wuhan University of Technology, Wuhan 430070, China.

Deformation and heat treatment are important means to strengthen aluminum alloys. However, the influence mechanism of pre-strain on aging precipitation kinetics and its effect on mechanical properties are still not clear. In this work, uniaxial isothermal tensile tests with different strains and artificial aging treatments for Al-Zn-Mg-Cu alloys have been carried out. Then, a model describing the precipitates kinetic behavior has been developed to investigate the effect of thermal pre-strain on subsequent aging precipitation kinetics and peak aging microhardness based on the microstructure characterization by TEM, SAXS and XRD tests. In addition, the role of dislocations on the aging precipitation kinetics is also explored. The experimental results show that the peak aging microhardness of the Al-Zn-Mg-Cu alloy reveals a tendency to decrease and increase and then the peak aging time firstly decreases and then keeps almost constant with the increase in the strain. The calculations demonstrate that the precipitate average size almost remains unchanged, while the precipitate volume fraction decreases and then increases with the increase in strain, which is consistent with the change in peak aging microhardness. It also indicates that dislocations can promote precipitate nucleation and growth, while the actual effect depends on the dislocation density, which is closely dependent on the pre-deformation condition, especially for the precipitate nucleation. In particular, when the dislocation density after thermal pre-deformation is not enough, it will slightly inhibit precipitate nucleation but promote precipitate growth, which could shorten the peak aging time, with the peak aging strength being guaranteed.
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http://dx.doi.org/10.3390/ma15134634DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9267310PMC
July 2022

An interconnected NaTi(PO)/carbon composite from an all-integrated framework with chelating Ti in a cross-linked citric acid-organic phosphonic acid skeleton for high-performance sodium storage.

J Colloid Interface Sci 2022 Jun 25;626:1-12. Epub 2022 Jun 25.

Automotive Engineering Research Institute, Jiangsu University, 301 Xuefu Road, Jingkou District, Zhenjiang 212013, PR China.

Finding suitable hosts for Na ion storage holds the key to achieving large-scale applications of sodium-ion batteries (SIBs). NaTi(PO) is widely considered to be an advanced anode material for SIBs, because of its 3D open framework, high theoretical capacity, and good thermodynamic stability. However, the instability of electrolyte/electrode interface and intrinsic low electronic conductivity of NaTi(PO) lead to the poor cycling and rate performance. In this work, an all-integrated framework with chelating Ti in a cross-linked citric acid-organic phosphonic acid skeleton is fabricated as a precursor for the synthesis of NaTi(PO)/carbon composite. The generated interconnected carbon provides extensive support for NaTi(PO) crystal. These unique structure delivers a high reversible capacity (225.8 mAh g at 0.2 A g), good rate performance (219.7 mAh g at 0.4 A g, and 189.6 mAh g at 1 A g), and superb long-term cycling stability (156.0 mAh g at 2 A g after 4000 cycles). It is believed that this facile and effective strategy can shed light on the development of advanced phosphate electrode materials for SIBs.
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http://dx.doi.org/10.1016/j.jcis.2022.06.123DOI Listing
June 2022

An interconnected NaTi(PO)/carbon composite from an all-integrated framework with chelating Ti in a cross-linked citric acid-organic phosphonic acid skeleton for high-performance sodium storage.

J Colloid Interface Sci 2022 Jun 25;626:1-12. Epub 2022 Jun 25.

Automotive Engineering Research Institute, Jiangsu University, 301 Xuefu Road, Jingkou District, Zhenjiang 212013, PR China.

Finding suitable hosts for Na ion storage holds the key to achieving large-scale applications of sodium-ion batteries (SIBs). NaTi(PO) is widely considered to be an advanced anode material for SIBs, because of its 3D open framework, high theoretical capacity, and good thermodynamic stability. However, the instability of electrolyte/electrode interface and intrinsic low electronic conductivity of NaTi(PO) lead to the poor cycling and rate performance. In this work, an all-integrated framework with chelating Ti in a cross-linked citric acid-organic phosphonic acid skeleton is fabricated as a precursor for the synthesis of NaTi(PO)/carbon composite. The generated interconnected carbon provides extensive support for NaTi(PO) crystal. These unique structure delivers a high reversible capacity (225.8 mAh g at 0.2 A g), good rate performance (219.7 mAh g at 0.4 A g, and 189.6 mAh g at 1 A g), and superb long-term cycling stability (156.0 mAh g at 2 A g after 4000 cycles). It is believed that this facile and effective strategy can shed light on the development of advanced phosphate electrode materials for SIBs.
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http://dx.doi.org/10.1016/j.jcis.2022.06.123DOI Listing
June 2022

A study protocol of a randomized phase II trial of perioperative chemoimmunotherapy verses perioperative chemoimmunotherapy plus preoperative chemoradiation for locally advanced gastric (G) or gastroesophageal junction (GEJ) adenocarcinoma: the NeoRacing study.

BMC Cancer 2022 Jun 28;22(1):710. Epub 2022 Jun 28.

Department of Radiation Oncology, Fudan University Shanghai Cancer Center, 270 Dong'an Road, Shanghai, 200032, People's Republic of China.

Background: Perioperative chemotherapy (ChT) and preoperative chemoradiation (CRT) are both the standard treatments for locally advanced gastric cancer (LAGC). CRT can achieve a higher pathological complete regression (pCR) rate, but whether this higher pCR rate can be transformed into a long-term survival benefit remains inconclusive. Therefore, relevant studies are in progress. On the other hand, immunotherapy has been established for the first-line treatment of advanced gastric cancer (AGC) and has been widely explored in the perioperative setting. The combination of chemotherapy/radiotherapy and immunotherapy may have a synergistic effect, which will lead to a better antitumor effect. The preliminary reports of ongoing studies show promising results, including a further improved pCR rate. However, the preferred treatment combination for LAGC is still not established. To solve this problem, we are carrying out this randomized phase II trial, which aims to evaluate the efficacy and safety of perioperative chemotherapy plus the use of PD-1 antibody with or without preoperative chemoradiation for LAGC.

Methods: Eligible patients with LAGC or gastroesophageal junction (GEJ) adenocarcinoma were randomized to receive perioperative ChT, PD-1 antibody, surgery with (Arm A) or without preoperative CRT (Arm B), and PD-1 antibody maintenance until one year after surgery. The primary endpoint of this study is that the pCR rate of Arm A will be significantly higher than that of Arm B. The secondary endpoints include the pathological partial regression (pPR) rate, R0 resection rate, objective response rate (ORR), event-free survival (EFS), overall survival (OS), safety and surgical complications. Moreover, several explorative endpoints will be evaluated to find and validate the predictive biomarkers of immunotherapy.

Discussion: The results of the NeoRacing study will provide important information concerning the application of PD-1 antibody in LAGC patients during the perioperative setting. Meanwhile, the two treatment protocols will be compared in terms of efficacy and safety.

Trial Registration: ClinicalTrials.gov , NCT05161572 . Registered 17 December 2021 - Retrospectively registered.
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http://dx.doi.org/10.1186/s12885-022-09786-9DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9238164PMC
June 2022

Cyclic immonium ion of lactyllysine reveals widespread lactylation in the human proteome.

Nat Methods 2022 07 27;19(7):854-864. Epub 2022 Jun 27.

Jiangsu Provincial Key Laboratory of Drug Metabolism and Pharmacokinetics, State Key Laboratory of Natural Medicines, China Pharmaceutical University, Nanjing, China.

Lactylation was initially discovered on human histones. Given its nascence, its occurrence on nonhistone proteins and downstream functional consequences remain elusive. Here we report a cyclic immonium ion of lactyllysine formed during tandem mass spectrometry that enables confident protein lactylation assignment. We validated the sensitivity and specificity of this ion for lactylation through affinity-enriched lactylproteome analysis and large-scale informatic assessment of nonlactylated spectral libraries. With this diagnostic ion-based strategy, we confidently determined new lactylation, unveiling a wide landscape beyond histones from not only the enriched lactylproteome but also existing unenriched human proteome resources. Specifically, by mining the public human Meltome Atlas, we found that lactylation is common on glycolytic enzymes and conserved on ALDOA. We also discovered prevalent lactylation on DHRS7 in the draft of the human tissue proteome. We partially demonstrated the functional importance of lactylation: site-specific engineering of lactylation into ALDOA caused enzyme inhibition, suggesting a lactylation-dependent feedback loop in glycolysis.
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http://dx.doi.org/10.1038/s41592-022-01523-1DOI Listing
July 2022

Sensory error drives fine motor adjustment.

Proc Natl Acad Sci U S A 2022 Jul 27;119(27):e2201275119. Epub 2022 Jun 27.

Hubei Key Laboratory of Genetic Regulation & Integrative Biology, School of Life Sciences, Central China Normal University, Wuhan 430079, China.

Fine audiovocal control is a hallmark of human speech production and depends on precisely coordinated muscle activity guided by sensory feedback. Little is known about shared audiovocal mechanisms between humans and other mammals. We hypothesized that real-time audiovocal control in bat echolocation uses the same computational principles as human speech. To test the prediction of this hypothesis, we applied state feedback control (SFC) theory to the analysis of call frequency adjustments in the echolocating bat, . This model organism exhibits well-developed audiovocal control to sense its surroundings via echolocation. Our experimental paradigm was analogous to one implemented in human subjects. We measured the bats' vocal responses to spectrally altered echolocation calls. Individual bats exhibited highly distinct patterns of vocal compensation to these altered calls. Our findings mirror typical observations of speech control in humans listening to spectrally altered speech. Using mathematical modeling, we determined that the same computational principles of SFC apply to bat echolocation and human speech, confirming the prediction of our hypothesis.
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http://dx.doi.org/10.1073/pnas.2201275119DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9271198PMC
July 2022

Peroxygenase-Catalyzed Selective Synthesis of Calcitriol Starting from Alfacalcidol.

Antioxidants (Basel) 2022 May 25;11(6). Epub 2022 May 25.

Tianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences, Tianjin 300308, China.

Calcitriol is an active analog of vitamin D3 and has excellent physiological activities in regulating healthy immune function. To synthesize the calcitriol compound, the concept of total synthesis is often adopted, which typically involves multiple steps and results in an overall low yield. Herein, we envisioned an enzymatic approach for the synthesis of calcitriol. Peroxygenase from (UPO) was used as a catalyst to hydroxylate the C-H bond at the C-25 position of alfacalcidol and yielded the calcitriol in a single step. The enzymatic reaction yielded 80.3% product formation in excellent selectivity, with a turnover number up to 4000. In a semi-preparative scale synthesis, 72% isolated yield was obtained. It was also found that UPO is capable of hydroxylating the C-H bond at the C-1 position of vitamin D3, thereby enabling the calcitriol synthesis directly from vitamin D3.
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http://dx.doi.org/10.3390/antiox11061044DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9220053PMC
May 2022

Development and Characterization of - 6S Recombinants Harboring a Novel Powdery Mildew Resistance Gene .

Front Plant Sci 2022 2;13:918508. Epub 2022 Jun 2.

State Key Laboratory of Wheat and Maize Crop Science, Henan Agricultural University, Zhengzhou, China.

Powdery mildew of wheat is a foliar disease that is spread worldwide. Cultivation of resistant varieties is the most effective, economical, and environmentally friendly strategy to curb this disease. Powdery mildew resistance genes () are the primary resources for resistance breeding, and new genes are in constant demand. Previously, we identified chromosome 6S#3 as a carrier of powdery mildew resistance and designated the resistance gene as . Here, we reported the design of 24 markers specific to 6S#3 on the basis of the full-length cDNA sequences of 6S#3 donor . accession TA1910, and the development of wheat- 6S#3 introgression stocks by induced homoeologous recombination. Further, 6S#3 introgression lines were identified and characterized by integration analysis of powdery mildew responses, hybridization, and molecular markers and was mapped to a distal interval of 42.80 Mb between markers Ael58410 and Ael57699 in the long arm of 6S#3. Two resistant recombinants, R43 (T6BS.6BL-6S#3L) and T27 (Ti6AS.6AL-6S#3L-6AL), contained segments harboring with less than 8% of 6S#3 genomic length, and two markers were diagnostic for . This study broadened powdery mildew resistance gene resources for wheat improvement and provided a fundamental basis for fine mapping and cloning of to further understand its molecular mechanism of disease resistance.
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http://dx.doi.org/10.3389/fpls.2022.918508DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9201914PMC
June 2022

YAP knockdown in combination with ferroptosis induction increases the sensitivity of HOS human osteosarcoma cells to pyropheophorbide-α methyl ester-mediated photodynamic therapy.

Photodiagnosis Photodyn Ther 2022 Jun 12;39:102964. Epub 2022 Jun 12.

Department of Orthopedics, The First Affiliated Hospital of Chongqing Medical University, Yuzhong, Chongqing 400016, China; Orthopedic Laboratory of Chongqing Medical University, Yuzhong, Chongqing 400016, China. Electronic address:

Background And Aims: This study was designed to explore the effects of Yes-associated protein (YAP) knockdown on human osteosarcoma (HOS) cell sensitivity to Pyropheophorbide-α methyl ester-mediated photodynamic therapy (MPPa-PDT), and to assess how YAP silencing in combination with treatment with the ferroptosis inducer Erastin improves HOS cell sensitivity to MPPa-PDT in an effort to better clarify the molecular mechanisms underlying these phenotypes.

Methods: At 12 h post-MPPa-PDT, Hoechst staining and flow cytometry were conducted to evaluate the apoptotic death of HOS cells. The expression of YAP in these cells at 12 h post-MPPa-PDT treatment was assessed via Western blotting and immunofluorescent staining. BODIPY-C11 was used to evaluate lipid peroxidation. Following shYAP lentiviral transduction, Western blotting was conducted to assess the expression of proteins associated with proliferation, apoptosis, and ferroptosis. EdU assays and clonogenic assays were performed to analyze cellular proliferation. Erastin-treated HOS cells were used to establish a ferroptosis model. Western blotting was used to measure ferroptosis-associated protein levels following shYAP and erastin treatment, while changes in proliferation and MDA levels in each group were examined using an MDA kit.

Results: At 12 h post-MPPa-PDT, HOS cells exhibited apoptotic characteristics including nuclear fragmentation and pyknosis, with concomitant increases in apoptosis-associated proteins as detected via Western blotting and apoptotic induction as measured via flow cytometry. Phosphorylated YAP levels fell and non-phosphorylated YAP levels rose following such treatment. Transfection with shYAP was successful as a means of generating stable HOS cell lines, and Western blotting analyses of these cells revealed reductions in proteins associated with cellular proliferation together with the upregulation of apoptosis-related proteins.  MDA assays indicated that erastin combined with YAP knockdown enhanced the sensitivity of HOS cells to MPPa-PDT treatment.

Conclusions: These data indicate that ferroptosis and YAP knockdown can enhance osteosarcoma cell sensitivity to MPPa-PDT therapy.
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http://dx.doi.org/10.1016/j.pdpdt.2022.102964DOI Listing
June 2022

Wheat heat shock factor TaHsfA2d contributes to plant responses to phosphate deficiency.

Plant Physiol Biochem 2022 Aug 8;185:178-187. Epub 2022 Jun 8.

College of Life Sciences/National Key Laboratory of Wheat and Maize Crop Science, Henan Agricultural University, Zhengzhou, 450002, China. Electronic address:

Phosphate (Pi) availability has become a major constraint limiting crop growth and production. Heat shock factors (Hsfs) play important roles in mediating plant resistance to various environmental stresses, including heat, drought and salinity. However, whether members of the Hsf family are involved in the transcriptional regulation of plant responses to Pi insufficiency has not been reported. Here, we identified that TaHsfA2d, a member of the heat shock factor family, was strongly repressed by Pi deficiency. Overexpressing TaHsfA2d-4A in Arabidopsis results in significantly enhanced sensitivity to Pi deficiency, evidenced by increased anthocyanin content, decreased proliferation and elongation of lateral roots, and reduced Pi uptake. Furthermore, RNA-seq analyses showed that TaHsfA2d-4A functions through up-regulation of a number of genes involved in stress responses and flavonoid biosynthesis. Collectively, these results provide evidence that TaHsfA2d participates in the regulation of Pi deficiency stress, and that TaHsfA2d could serve as a valuable gene for genetic modification of crop tolerance to Pi starvation.
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http://dx.doi.org/10.1016/j.plaphy.2022.05.035DOI Listing
August 2022

Toll like receptor 4 gene Asp299Gly polymorphism increases the risk of diabetic microvascular complications: a meta analysis.

Diabetol Metab Syndr 2022 Jun 7;14(1):79. Epub 2022 Jun 7.

Department of Endocrinology, The First Hospital of Lanzhou University, No. 1 Donggang West Road, Lanzhou, 730000, Gansu, People's Republic of China.

Objective: The relationship between Toll like receptor 4(TLR4) gene Asp299Gly polymorphism and diabetic microvascular complications (DMI) is unclear. Therefore, the aim of this meta analysis was to explore the relationship between TLR4 Asp299Gly polymorphism and DMI.

Methods: System search PubMed, Web of science, Springer, Cochrane library, ELSEVIER, Wanfang database, VIP, CNKI, a case-control study of the correlation between TLR4 gene Asp299Gly polymorphism and DMI published before June 2020 was collected.

Results: We included 6 articles, a total of 11 studies involving patients with type 2 diabetes mellitus (T2DM) complicated by microvascular complications 1834 cases, without corresponding microvascular complications 4069 cases. TLR4 gene Asp299Gly polymorphism increased the risk of microvascular complications in T2DM (dominant model OR = 1.52, 95% CI 1.10-2.09, p = 0.01; allelic model OR = 1.42, 95% CI 1.02-1.96, p = 0.04). Subgroup analysis by race and different type of microvascular complications, we found that TLR4 gene Asp299Gly polymorphism was associated with increased risk of microvascular complications in the Caucasian population (dominant model OR = 1.69, 95% CI 1.22-2.35, P = 0.002; allelic model OR = 1.56, 95% CI 1.10-2.21, P = 0.01) and increased the risk of retinopathy in patients with T2DM(dominant model OR = 1.81, 95% CI 1.04-3.14, P = 0.03; allelic model OR = 1.77, 95% CI 1.05-2.98, P = 0.03).

Conclusion: TLR4 gene Asp299Gly polymorphism was associated with increased risk of microvascular complications in patients with T2DM, especially diabetic retinopathy (DR).
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http://dx.doi.org/10.1186/s13098-022-00849-2DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9172045PMC
June 2022

Achieving Balanced Electrical Performance of Host Material through Dual N-P═O Resonance Linkage for Efficient Electroluminescence.

ACS Appl Mater Interfaces 2022 Jun 24;14(22):25834-25841. Epub 2022 May 24.

State Key Laboratory of Organic Electronics and Information Displays, Institute of Advanced Materials (IAM), Nanjing University of Posts & Telecommunications, 9 Wenyuan Road, Nanjing 210023, China.

Developing high-performance host materials is one of the biggest challenges for blue and white thermally activated delayed-fluorescence (TADF) organic light-emitting diode (OLED) technology due to the rigorous requirements of both efficient carrier flux ability and high triplet energy () levels in static donor-acceptor molecules. Here, with the aid of a dual-resonance strategy, a host molecule showing dynamic adaption features in the acceptor-resonance-donor-resonance-acceptor (A-r-D-r-A) molecular configuration has been successfully developed through the implantation of two acceptors of diphenylphosphine oxide into electron-donating 5,10-dihydrophenazine with N-P═O resonance linkages. Owing to the dual enantiotropic N═P-O resonances, the designed A-r-D-r-A molecule exhibits an extraordinarily balanced charge flux transportation attribute at high (2.96 eV). Excitingly, blue and warm-white TADF OLEDs hosted by the A-r-D-r-A molecule exhibit outstanding external quantum efficiencies of 14.7 and 20.3%, respectively. Our studies not only broaden the scope of resonance molecules but also indicate that a resonance structure is an effective linkage to develop optoelectronic materials with dynamically adaptive properties.
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http://dx.doi.org/10.1021/acsami.2c02745DOI Listing
June 2022

Dissociation of ammonia borane and its subsequent nucleation on the Ru(0001) surface revealed by density functional theoretical simulations.

Phys Chem Chem Phys 2022 May 25;24(20):12226-12235. Epub 2022 May 25.

Henan Key Laboratory of Materials on Deep-Earth Engineering, School of Materials Science and Engineering, Henan Polytechnic University, Henan 454003, China.

The chemical vapor deposition method is widely used in preparation of graphene, hexagonal boron nitride (h-BN) and other 2D materials. To improve the quality of h-BN, it is essential to learn details of the growth mechanism including dissociation of precursors, the initial nucleation, the stabilities of various clusters and the origins behind them. Here, the dissociation process of ammonia borane (AB) and its later nucleation on the Ru(0001) surface were simulated with density functional theory. The results show that the key step before dissociation is the chemical adsorption of AB molecule on the Ru(0001) surface. In the approaching process of AB molecule to Ru(0001), all bonds connected with H, irrespective of B-H or N-H, are stretched by the underlying metal surface. The H atoms connected with boron prefer to be dissociated followed by those connected to nitrogen. After full dehydration, BN dimer accumulates by forming chain-like, ring-shaped and honeycomb clusters. The chain-like geometries are energy-favoured in BN clusters with size lower than 6. Beyond this, the honeycomb configuration becomes the energy-favoured one, resulting in a geometry evolution from chain-like to honeycomb. The nucleation barrier and the critical nuclei size of BN clusters on Ru(0001) at different chemical potentials are discussed, which can be realized by tuning ratios of B to N in the feedstock. The growth mode observed for h-BN on Ru(0001) can be easily extended to growth on other transition metals and other 2D materials, thus should provide important information in optimizing experimental schemes.
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http://dx.doi.org/10.1039/d1cp05957bDOI Listing
May 2022

State-independent and -dependent behavioral and neuroelectrophysiological characteristics during dynamic decision-making in patients with current and remitted depression.

J Affect Disord 2022 07 25;309:85-94. Epub 2022 Apr 25.

Medical Psychological Center, The Second Xiangya Hospital, Central South University, Changsha, Hunan, China; National Clinical Research Center for Mental Disorders, Changsha, Hunan, China; Medical Psychological Institute of Central South University, Changsha 410011, China. Electronic address:

Background: It is unclear whether the altered decision-making (DM) observed in patients with major depressive disorder (MDD) is neurophysiological and whether it improves with remission of depressive symptoms. The aim of this study was to identify developmental patterns of DM behavior, related cognitive characteristics, and electrophysiological abnormalities in patients with MDD across clinical stages.

Methods: A sample of 48 first-episode MDD patients (FD group), 41 remitted MDD patients (RD group), and 43 healthy controls (HCs) completed psychometric assessments and performed the balloon analogue risk task (BART) while event-related potentials (ERPs) were recorded.

Results: The RD group had lower depressiveness, self-blame, rumination, and catastrophizing tendencies, and higher mental resilience scores than the FD group, but retained significant differences from HCs. MDD patients showed a more conservative DM strategy than HCs, with no significant difference between the FD and RD groups. Compared to the FD group, the RD group had a smaller FRN for negative feedback and a trend toward a smaller P3 for positive feedback. Compared with HCs, the RD group had a smaller P3 during the positive feedback phase. FRN amplitude correlated positively with depression level and negatively with mental resilience.

Limitations: Because a comparative cross-section design was employed, longitudinal studies are needed to make causal inferences.

Conclusion: MDD patients presented a stable risk-avoidance bias in actively depressed and remission periods, consistent with a state-independent impairment pattern. Significantly reduced FRN amplitudes during remission indicated a state-dependent impairment pattern, and FRN amplitudes correlated with depression level. An abnormal feedback P3 component may be a state-independent characteristic that may become more pronounced with MDD progression.
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http://dx.doi.org/10.1016/j.jad.2022.04.120DOI Listing
July 2022

Design of a genetically programmed barnacle-curli inspired living-cell bioadhesive.

Mater Today Bio 2022 Mar 8;14:100256. Epub 2022 Apr 8.

Key Lab of Molecular Biophysics of Ministry of Education, College of Life Science and Technology Huazhong University of Science and Technology, Wuhan, China.

In nature, barnacles and bacterial biofilms utilize self-assembly amyloid to achieve strong and robust interface adhesion. However, there is still a lack of sufficient research on the construction of macroscopic adhesives based on amyloid-like nanostructures through reasonable molecular design. Here, we report a genetically programmed self-assembly living-cell bioadhesive inspired by barnacle and curli system. Firstly, the encoding genes of two natural adhesion proteins (CsgA and cp19k) derived from curli and barnacle cement were fused and expressed as a fundamental building block of the bioadhesive. Utilizing the natural curli system of , fusion protein can be delivered to cell surface and self-assemble into an amyloid nanofibrous network. Then, the cells were incorporated into the molecular chain network of xanthan gum (XG) through covalent conjugation to produce a living-cell bioadhesive. The shear adhesive strength of the bioadhesive to the surface of the aluminum sheet reaches 278 ​kPa. Benefiting from living cells encapsulated inside, the bioadhesive can self-regenerate with adequate nutrients. This adhesive has low toxicity to organisms, strong resistance to the liquid environment in vivo, easy to pump, exhibiting potential application prospects in biomedical fields such as intestinal soft tissue repair.
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http://dx.doi.org/10.1016/j.mtbio.2022.100256DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9034392PMC
March 2022

Chemoenzymatic Hunsdiecker-Type Decarboxylative Bromination of Cinnamic Acids.

ACS Catal 2022 Apr 4;12(8):4554-4559. Epub 2022 Apr 4.

Department of Biotechnology, Delft University of Technology, Van der Maasweg 9, Delft 2629HZ, The Netherlands.

In this contribution, we report chemoenzymatic bromodecarboxylation (Hunsdiecker-type) of α,ß-unsaturated carboxylic acids. The extraordinarily robust chloroperoxidase from (VCPO) generated hypobromite from HO and bromide, which then spontaneously reacted with a broad range of unsaturated carboxylic acids and yielded the corresponding vinyl bromide products. Selectivity issues arising from the (here undesired) addition of water to the intermediate bromonium ion could be solved by reaction medium engineering. The vinyl bromides so obtained could be used as starting materials for a range of cross-coupling and pericyclic reactions.
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http://dx.doi.org/10.1021/acscatal.2c00485DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9016706PMC
April 2022

Year-round observation of atmospheric inorganic aerosols in urban Beijing: Size distribution, source analysis, and reduction mechanism.

J Environ Sci (China) 2022 Apr 21;114:354-364. Epub 2022 Feb 21.

State Key Laboratory of Environmental Criteria and Risk Assessment, Chinese Research Academy of Environmental Sciences, Beijing 100012, China.

To investigate particle characteristics and find an effective measure to control severe particle pollution, year-round observation of size-segregated inorganic aerosols was conducted in Beijing from January to December, 2016. The sampled atmospheric particles all presented bimodal size distribution at four pollution levels (clear, slight pollution, moderate pollution and severe pollution), and peak values appeared at the size range of 0.7-2.1 μm and >9.0 μm, respectively. As dominant particle compositions, NO, SO, and NH in four pollution levels all showed significant peaks in fine mode, especially at the size range of 1.1-2.1 μm. Secondary inorganic aerosols accounted for about 67.6% (36.3% (secondary sulfates) + 31.3% (secondary nitrates)) of the total sources of fine particles in urban Beijing. Severe pollution of fine particles was mainly caused by the air masses transported from nearby western and southern areas, which are industrial and densely populated region, respectively. Sensitivity tests further revealed that the control measures focusing on ammonium emission reduction was the most effective for particle pollution mitigation, and fine particles all showed nonlinear responses after reducing ammonium, nitrate, and sulfate concentrations, with the fitting curves of y = -120.8x - 306.1x + 290.2x, y = -43.5x - 67.8x, and y = -25.8x - 110.4x + 7.6x, respectively (y and x present fine particle mass variation (μg/m) and concentration reduction ratio (CRR)/100 (dimensionless)). Overall, our study presents useful information for understanding the characteristics of atmospheric inorganic aerosols in urban Beijing, as well as offers policy makers with effective measure for mitigating particle pollution.
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http://dx.doi.org/10.1016/j.jes.2021.09.014DOI Listing
April 2022

A statistic comparison of multi-element analysis of low atmospheric fine particles (PM) using different spectroscopy techniques.

J Environ Sci (China) 2022 Apr 14;114:194-203. Epub 2022 Jan 14.

State Key Laboratory of Environmental Criteria and Risk Assessment, Chinese Research Academy of Environmental Sciences, Beijing 100012, China.

Over the past few decades, the metal elements (MEs) in atmospheric particles have aroused great attention. Some well-established techniques have been used to measure particle-bound MEs. However, each method has its own advantages and disadvantages in terms of complexity, accuracy, and specific elements of interest. In this study, the performances of inductively coupled plasma-optical emission spectrometry (ICP-OES) and total reflection X-ray fluorescence spectroscopy (TXRF) were evaluated for quality control to analyze data accuracy and precision. The statistic methods (Deming regression and significance testing) were applied for intercomparison between ICP-OES and TXRF measurements for same low-loading PM samples in Weizhou Island. The results from the replicate analysis of standard filters (SRM 2783) and field filters samples indicated that 10 MEs (K, Ca, V, Cr, Mn, Fe, Ni, Cu, Zn, and Pb) showed good accuracies and precision for both techniques. The higher accuracy tended to the higher precision in the MEs analysis process. In addition, the interlab comparisons illustrated that V and Mn all had good agreements between ICP-OES and TXRF. The measurements of K, Cu and Zn were more reliable by TXRF analysis for low-loading PM. ICP-OES was more accurate for the determinations for Ca, Cr, Ni and Pb, owing to the overlapping spectral lines and low sensitivity during TXRF analysis. The measurements of Fe, influenced by low-loading PM, were not able to determine which instrument could obtain more reliable results. These conclusions could provide reference information to choose suitable instrument for the determination of MEs in low-loading PM samples.
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http://dx.doi.org/10.1016/j.jes.2021.08.034DOI Listing
April 2022

Catalytic hairpin activated gold-magnetic/gold-core-silver-shell rapid self-assembly for ultrasensitive Staphylococcus aureus sensing via PDMS-based SERS platform.

Biosens Bioelectron 2022 Aug 3;209:114240. Epub 2022 Apr 3.

School of Food and Biological Engineering, Jiangsu University, Zhenjiang, 212013, People's Republic of China; College of Food and Biological Engineering, Jimei University, Xiamen, 361021, People's Republic of China. Electronic address:

Staphylococcus aureus (S. aureus) has been identified as a marker of food contamination, closely associated with human health. This work designs a sensitive and rapid bio-detection strategy for S. aureus based on hybridization chain reaction-assisted surface enhanced Raman scattering (HCR-assisted-SERS) signal amplification. In this approach, the interaction between the aptamer (Apt) and its partial complementary DNA strands (cDNA) fabricated on the surface of gold-assisted magnetic nanoparticles (Au-MNPs) and the subsequent detachment of the cDNA results in the activation of the HCR process. In the HCR, a pair of hairpin structured DNA probes (H1 and H2) with sticky ends self-assembles to form a long DNA polymer. Subsequently, the output and amplification of the SERS signal were performed by conjugating 4-ATP modified [email protected] NPs with the obtained DNA polymer via a specific Ag-S bond, and further collected through a self-administered polydimethylsiloxane (PDMS) cone-shaped support array. The precise quantification of S. aureus was performed in the concentration range of 28 to 2.8 × 10 cfu/mL, achieving a detection limit of 0.25 cfu/mL. This strategy was further applied to S. aureus detection in spiked milk samples with good recoveries (91-102%) and the relative standard deviation (4.35-8.41%). The sensing platform also showed satisfactory validation results (p > 0.05) using the traditional plate counting method. The proposed HCR-assisted SERS probe can be extended to other foodborne pathogenic bacteria types via engineering appropriate Apt and DNA initiators, thus, inspiring widespread applications in food safety and biomedical research.
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http://dx.doi.org/10.1016/j.bios.2022.114240DOI Listing
August 2022

Fraud detection in crude palm oil using SERS combined with chemometrics.

Food Chem 2022 Sep 15;388:132973. Epub 2022 Apr 15.

School of Food and Biological Engineering, Jiangsu University, Zhenjiang 212013, PR China; College of Food and Biological Engineering, Jimei University, Xiamen 361021, PR China. Electronic address:

Edible crude palm oil (CPO) is a vital oil utilized in various industries, including food, pharmaceuticals, and domestic cooking. Unfortunately, reports of CPO adulteration with harmful Sudan dyes have surfaced over the years. Surface-enhanced Raman spectroscopy (SERS) and chemometrics were employed to detect Sudan dyes adulteration in CPO within 900 - 1800 cm Raman peak. The concentration of Sudan dyes detected in CPO samples ranged between 0.005 and 4 ppm. The principal component analysis (PCA) model detected Sudan II and Sudan IV in CPO with 99.88 and 99.90% accuracy. Linear discriminant analysis (LDA) and K-Nearest Neighbors (KNN) also recorded high detection rates of Sudan II and IV dyes in CPO. Sudan II and IV dyes could be detected at 0.0028 ppm and 0.0019 ppm by this sensor. The performance of the [email protected] SERS sensor was comparable to that of HPLC. This study proved SERS and chemometrics can be used to authenticate edible CPO.
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http://dx.doi.org/10.1016/j.foodchem.2022.132973DOI Listing
September 2022
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