Publications by authors named "Huang Pan"

121 Publications

A quadruple protection procedure for resuming pig production in small-scale ASFV-positive farms in China.

Curr Res Microb Sci 2021 Dec 26;2:100014. Epub 2020 Nov 26.

Laboratory of Molecular Virology and Immunology, Innovation Technology Center, Haid Research Institute, Guangdong Haid Group Co., Ltd, Guangzhou, China.

African swine fever (ASF) outbreak has caused serious economic losses in Asia since 2018. As ASF is a new emerging disease, many farmers hesitate to raise pigs before biosafety procedures were evaluated to be effective. To support small-scale farms in resuming pig production, a comprehensive procedure, called the quadruple protection procedure (QPP), was tested in 35 small farms which had been confirmed with African swine fever virus (ASFV). The QPP takes care of the farms' construction, environmental disinfection, regular immunization, and feed quality. Qualified daily management was supplemented as well. During a one-year survey four disinfectants and one piece of equipment were used in higher frequency. A 7- or 15-day empty period after the disinfection was suitable when it was combined with the rest of the protection measures from QPP. Totally 18,730 porkers and 3,006 sows were healthy by the end of the study with percentage of 100 and 98.8, respectively, indicating that QPP could protect pigs in small-scale farms from pathogens within China. This study developed an effective protective procedure system for small-scale farms to produce pigs under the risk of ASF outbreak.
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http://dx.doi.org/10.1016/j.crmicr.2020.100014DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8610312PMC
December 2021

Effects of and a Bacteriophage Cocktail on Growth Performance, Serum Biochemistry, Digestive Enzyme Activities, Intestinal Morphology, Immune Responses, and the Intestinal Microbiota in Rabbits.

Antibiotics (Basel) 2021 Nov 4;10(11). Epub 2021 Nov 4.

Institute of Animal Husbandry and Veterinary Medicine, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, China.

The objective of this study was to assess the effects of dietary supplementation with (CB) and a bacteriophage cocktail (BP) on growth performance, serum biochemical parameters, intestinal digestive and oxidase enzymes, intestinal morphology, immune responses, and the cecum microbiota in rabbits. In total, 108 New Zealand rabbits (5 weeks old) were randomly and equally allotted into three dietary treatment groups (four replicates per treatment, n = 36/treatment): (1) the control (CN) group-rabbits fed the basal diet; (2) CB group-rabbits fed the basal diet supplemented with 100 mg/kg diet ; and (3) BP group-rabbits fed the basal diet supplemented with 200 mg/kg diet BP cocktail, respectively, for 6 weeks. Compared with the CN diet, dietary CB and BP inclusion increased the average daily gain (ADG) and average daily feed intake (ADFI) and decreased the feed/gain (F/G) ratio of rabbits. Furthermore, CB increased the digestive enzyme activity (α-amylase and trypsin in the ileum); the chymotrypsin activity was also significantly increased in the duodenum and jejunum. Supplementation with CB significantly enhanced antioxidant capacity (SOD and GSH-Px) in the jejunum and ileum and reduced MDA levels. Additionally, rabbits fed CB had significantly elevated villus height (V) and (V/C) ratios but reduced crypt depth (C). Moreover, dietary CB supplementation markedly increased the ileal expression of tight junction proteins (occludin, ZO-1, and claudin-1) and increased secretory immunoglobulin A (sIgA) production. High-throughput sequencing indicated that the microbiota in the rabbit intestine was altered by CB and BP. Venn diagrams and heatmap plots revealed that the gut microbial community composition varied obviously among rabbits fed different diets. Specifically, CB increased the relative abundance of beneficial bacteria to maintain intestinal barrier homeostasis, whereas BP decreased the relative abundance of Gammaproteobacteria, which included a plenty of pathogenic bacteria.
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http://dx.doi.org/10.3390/antibiotics10111347DOI Listing
November 2021

Long-term treatment of Nicotinamide mononucleotide improved Age-related Diminished Ovary Reserve through enhancing the mitophagy level of granuloas cellsin mice.

J Nutr Biochem 2021 Nov 18:108911. Epub 2021 Nov 18.

School of Medicine, Jiangsu University, Zhenjiang 212013, PR China. Electronic address:

Ovarian aging affects the reproductive health of elderly women due to decline in oocyte quality, which is closely related to mitochondrial dysfunction. Nicotinamide mononucleotide (NMN), as a precursor of NAD, effectively regulate mitochondria metabolism in mice. However, roles of NMN in improving age-related diminished ovary reserve remain to be determined. In present study, 4, 8, 12, 24, 40-week old female ICR mice were collected and a 20-week-long administration of NMN was conducted to 40-week-old mice (60WN), meanwhile the control group is given water (60WC). First, we found that 20-week-long administration of NMN to 40-week-old mice exhibited anti-aging and anti-inflammatory effects on organ structures, along with the improvement of estrus cycle condition and endocrine function. The number of primordial, primary, secondary, antral follicles and corpora luteum of ovaries in 60WN group was significantly increased compared with those in 60WC group. Additionally, the protein and gene expressions of P16 of ovaries were significantly reduced in 60WN group than in 60WC group. the mitochondria biogenesis, autophagy level, and proteases activity enhanced in granulosa cells after 20-week-administration of NMN. Present results indicate that NMN has the potential to save Diminished Ovary Reserve by long-term treatment, providing a basis for exploring the role of NMN in anti-ovarian aging by enhancing the mitophagy level of granuloas cells.
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http://dx.doi.org/10.1016/j.jnutbio.2021.108911DOI Listing
November 2021

Histologic and biomechanical evaluation of the thoracolumbar fascia graft for massive rotator cuff tears in a rat model.

J Shoulder Elbow Surg 2021 Nov 11. Epub 2021 Nov 11.

Department of Orthopedic Surgery, Southwest Hospital, Army Military Medical University, Chongqing, China. Electronic address:

Background: Fascial autografts, which are easily available grafts, have provided a promising option in patients with massive rotator cuff tears. However, no fascial autografts other than the fascia lata have been reported, and the exact healing process of the fascia-to-bone interface is not well understood. The objective of this study is to histologically and biomechanically evaluate the effect of the thoracolumbar fascia (TLF) on fascia-to-bone healing.

Methods: A total of 88 rats were used in this study. Eight rats were sacrificed at the beginning to form an intact control group, and the other rats were divided randomly into 2 groups (40 rats per group): the thoracolumbar fascia augmentation group (TLF group) and the repair group (R group). The right supraspinatus was detached, and a 3*5 mm defect of the supraspinatus was created. The thoracolumbar fascia was used to augment the torn supraspinatus in the TLF group, whereas in the R group, the torn supraspinatus was repaired in only a transosseous manner. Histology and biomechanics were assessed at 1, 2, 4, 8 and 16 weeks postoperatively.

Results: The modified tendon maturation score of the TLF group was higher than that of the R group at 8 weeks (23.00 ± 0.71 vs. 24.40 ± 0.89, P=.025) and 16 weeks (24.60 ± 0.55 vs. 26.40 ± 0.55, P≤.001). The TLF group showed a rapid vascular reaction, and the peak value appeared at 1 week. Later, the capillary density decreased, and almost no angiogenesis was observed at 8 weeks postoperatively. Immunohistochemistry results demonstrated a significantly higher percentage of collagen I in the TLF group at 4, 8 and 16 weeks (24.78% ± 2.76% vs. 20.67% ± 2.11% at 4 weeks, p=.046; 25.46% ± 1.77% vs. 21.49% ± 2.33% at 8 weeks, p=.026; 34.77% ± 2.25% vs. 30.01% ± 3.17% at 16 weeks, p=.040) postoperatively. Biomechanical tests revealed that the ultimate failure force in the TLF group was significantly higher than that in the R group at the final evaluation (29.13 ± 2.49 N vs. 23.10 ± 3.47 N, p=.022).

Conclusions: The TLF autograft can promote a faster biological healing process and a better fixation strength. It could be used as an alternative reinforcement or bridging patch when the fascia lata is not appropriate or available for SCR.

Level Of Evidence: Basic Science Study; Histology and Biomechanics.
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http://dx.doi.org/10.1016/j.jse.2021.10.019DOI Listing
November 2021

MiR-6924-5p-rich exosomes derived from genetically modified Scleraxis-overexpressing PDGFRα(+) BMMSCs as novel nanotherapeutics for treating osteolysis during tendon-bone healing and improving healing strength.

Biomaterials 2021 Nov 5;279:121242. Epub 2021 Nov 5.

Department of Orthopedics/Sports Medicine Center, State Key Laboratory of Trauma, Burn and Combined Injury, First Affiliated Hospital of Third Military Medical University (Army Medical University), Chongqing, 400000, China. Electronic address:

Osteolysis at the tendon-bone interface can impair pullout strength during tendon-bone healing and lead to surgery failure, but the effects of clinical treatments are not satisfactory. Mesenchymal stem cell (MSC)-derived exosomes have been used as potent and feasible natural nanocarriers for drug delivery and have been proven to enhance tendon-bone healing strength, indicating that MSC-derived exosomes could be a promising therapeutic strategy. In this study, we explored Scleraxis (Scx) dynamically expressed in PDGFRα(+) bone marrow-derived mesenchymal stem cells (BMMSCs) during natural tendon-bone healing. Then, we investigated the role of PDGFRα(+) BMMSCs in tendon-bone healing after Scx overexpression as well as the underlying mechanisms. Our data demonstrated that Scx-overexpressing PDGFRα(+) BMMSCs (BMMSC) could efficiently inhibit peritunnel osteolysis and enhance tendon-bone healing strength by preventing osteoclastogenesis in an exosomes-dependent manner. Exosomal RNA-seq revealed that the abundance of a novel miRNA, miR-6924-5p, was highest among miRNAs. miR-6924-5p could directly inhibit osteoclast formation by binding to the 3'-untranslated regions (3'UTRs) of OCSTAMP and CXCL12. Inhibition of miR-6924-5p expression reversed the prevention of osteoclastogenic differentiation by BMMSC derived exosomes (BMMSC-exos). Local injection of BMMSC-exos or miR-6924-5p dramatically reduced osteoclast formation and improved tendon-bone healing strength. Furthermore, delivery of miR-6924-5p efficiently inhibited the osteoclastogenesis of human monocytes. In brief, our study demonstrates that BMMSC-exos or miR-6924-5p could serve as a potential therapy for the treatment of osteolysis during tendon-bone healing and improve the outcome.
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http://dx.doi.org/10.1016/j.biomaterials.2021.121242DOI Listing
November 2021

RANBP10 promotes glioblastoma progression by regulating the FBXW7/c-Myc pathway.

Cell Death Dis 2021 10 20;12(11):967. Epub 2021 Oct 20.

State Key Laboratory of Silkworm Genome Biology, Southwest University, 400716, Chongqing, China.

RAN binding protein 10 (RANBP10), a ubiquitously expressed and evolutionarily conserved protein, as a RAN-GTP exchange factor (GEF) to regulate several factors involved in cellular progression. Previous studies showed that RANBP10 was overexpressed in prostate cancer cells and was responsible for androgen receptor (AR) activation. However, the biological function of RANBP10 in glioblastoma (GBM) has not been studied. Here, we found that RANBP10 was overexpressed in GBM, and high RANBP10 expression was closely linked to poor survival of patients with GBM. Downregulation of RANBP10 significantly inhibited cell proliferation, migration, invasion, and tumor growth of GBM cells. In addition, we revealed that RANBP10 could suppress the promoter activity of FBXW7, and thereby increase the protein stability of c-Myc in GBM cells. Silencing of FBXW7 in RANBP10-knockdown GBM cells could partly negate the effects induced by RANBP10 downregulation. Taken together, our findings established that RANBP10 significantly promoted GBM progression by control of the FBXW7-c-Myc axis, and suggest that RANBP10 may be a potential target in GBM.
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http://dx.doi.org/10.1038/s41419-021-04207-4DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8528885PMC
October 2021

Adipogenic differentiation was inhibited by downregulation of PPARγ signaling pathway in aging tendon stem/progenitor cells.

J Orthop Surg Res 2021 Oct 18;16(1):614. Epub 2021 Oct 18.

Department of Sports Medicine Center, Southwest Hospital, Army Medical University, Chongqing, 400038, China.

Background: Tendon stem/progenitor cells (TSPCs) play a vital role in tendon repair and regeneration. Previously we found more adipocytes accumulated in the patellar tendon injury sites in aging rats compared with the young ones, of which the mechanism is still unknown. Here, we want to identify whether erroneous differentiation of TSPCs by aging accounts for the adipocyte accumulation.

Methods: TSPCs from young and aging rats were isolated and propagated. Both young and aging TSPCs were induced to differentiate into adipocytes, and Oil red O staining, quantitative real-time polymerase chain reaction (qRT-PCR), western-blot and immunofluorescent staining were used to evaluate the capability of TSPCs. RNA sequencing was utilized to screen out different genes and signaling pathways related to adipogenesis between young and aging TSPCs.

Results: The Oil red O staining showed there were more adipocytes formed in young TSPCs. Besides, adipogenic markers perilipin, peroxisome proliferator-activated receptor γ (PPARγ), CCAAT/enhancer-binding proteins alpha (C/EBPα) and Fatty acid-binding protein 4 (FABP4) were elevated both at gene and protein level. PPARγ signaling pathway was selected as our target via RNA sequencing. After adding the signaling activators, Rosiglitazone maleate (RM), inhibited adipogenesis of aging TSCs was reversed.

Conclusions: In conclusion, aging inhibited adipogenesis of TSPCs by down-regulating PPARγ signaling. It is not likely that the adipocyte accumulation in aging tendon during repair was due to the aging of TSPCs. This may provide new targets for curing aging tendon injuries or tendinopathies.
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http://dx.doi.org/10.1186/s13018-021-02720-yDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8522149PMC
October 2021

Occurrence and antimicrobial resistance of isolated from retail meats in Anhui, China.

Food Sci Nutr 2021 Sep 16;9(9):4701-4710. Epub 2021 Jul 16.

Engineering Research Center of Bio-process Ministry of Education Hefei University of Technology Hefei China.

is considered one of the major foodborne pathogens associated with severe infections. Little attempt has been focused on the distribution of in retail meats and the analysis of its phenotypic characteristics in Anhui Province. The aim of this study was to characterize the prevalence of serovars, antimicrobial susceptibility, antimicrobial resistance genes, and virulence genes in recovered from retail meats in Anhui, China. Out of the 120 samples collected from supermarket chains and open-air markets, 16 samples (13.3%) were positive for , of which serovars Enteritidis and Typhimurium were the common serotypes. Significant differences in incidence were found between supermarket chains and open-air markets ( < 0.05). Overall, all 16 isolates were resistant to at least two tested antimicrobials, while 12 isolates showed multiple antimicrobial resistant phenotypes. High resistance was observed for ampicillin (87.5%), doxycycline (75.0%), and tetracycline (62.5%). The was detected in all isolates, and the (93.8%) and the (81.3%) were predominant in 10 resistance genes conferring five classes of antimicrobials. In addition, the correlation between resistance phenotypes and genes of tetracyclines and aminoglycosides was more than 80%. Interestingly, all the isolates contained the genes , , , and , whereas the was variably represented. The findings in this study showed high prevalence, antimicrobial resistance, and the existence of virulence genes, suggesting that effective measures are required to ensure microbial safety from retail meats.
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http://dx.doi.org/10.1002/fsn3.2266DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8441314PMC
September 2021

Endogenous Taurine Downregulation Is Required for Renal Injury in Salt-Sensitive Hypertensive Rats via CBS/HS Inhibition.

Oxid Med Cell Longev 2021 25;2021:5530907. Epub 2021 Aug 25.

Department of Pediatrics, Peking University First Hospital, Beijing 100034, China.

Although taurine is known to exert an antihypertensive effect, it is unclear whether it is involved in the mechanism for hypertension-related target organ injury. To reveal the role of endogenous taurine in renal injury formation during salt-sensitive hypertension and clarify its mechanisms, both salt-sensitive Dahl rats and salt-resistant SS-13BN rats were fed a high-salt diet (8% NaCl) and given 2% taurine for 6 weeks. Rat systolic blood pressure (SBP) was measured by the tail-cuff method and artery catheterization. Kidney ultrastructure was observed under an electron microscope. Taurine content and mRNA and protein levels of taurine synthases, cysteine dioxygenase type 1 (CDO1) and cysteine sulfinic acid decarboxylase (CSAD), were decreased in Dahl rats fed a high-salt diet. However, taurine supplementation and the resulting increase in renal taurine content reduced the increased SBP and improved renal function and structural damage in high-salt diet-fed Dahl rats. In contrast, taurine did not affect SS-13BN SBP and renal function and structure. Taurine intervention increased the renal HS content and enhanced cystathionine--synthase (CBS) expression and activity in Dahl rats fed a high-salt diet. Taurine reduced the renin, angiotensin II, and aldosterone contents and the levels of oxidative stress indices in Dahl rat renal tissues but increased antioxidant capacity, antioxidant enzyme activity, and protein expression. However, taurine failed to achieve this effect in the renal tissue of SS-13BN rats fed a high-salt diet. Pretreatment with the CBS inhibitor HA or renal CBS knockdown inhibited HS generation and subsequently blocked the effect of taurine on renin, superoxide dismutase 1 (SOD1), and superoxide dismutase 2 (SOD2) levels in high-salt-stimulated Dahl renal slices. In conclusion, the downregulation of endogenous taurine production resulted in a decrease in the renal CBS/HS pathway. This decrease subsequently promoted renin-angiotensin-aldosterone system (RAAS) activation and oxidative stress in the kidney, ultimately contributing to renal injury in salt-sensitive Dahl rats.
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http://dx.doi.org/10.1155/2021/5530907DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8413057PMC
August 2021

FABNet: Fusion Attention Block and Transfer Learning for Laryngeal cancer Tumor Grading in P63 IHC Histopathology Images.

IEEE J Biomed Health Inform 2021 Sep 1;PP. Epub 2021 Sep 1.

Laryngeal cancer tumor (LCT) grading is a challenging task in P63 Immunohistochemical (IHC) histopathology images due to small differences between LCT levels in pathology images, the lack of precision in lesion regions of interest (LROIs) and the paucity of LCT pathology image samples. The key to solving the LCT grading problem is to transfer knowledge from other images and to identify more accurate LROIs, but the following problems occur: 1) transferring knowledge without a priori experience often causes negative transfer and creates a heavy workload due to the abundance of image types, and 2) convolutional neural networks (CNNs) constructing deep models by stacking cannot sufficiently identify LROIs, often deviate significantly from the LROIs focused on by experienced pathologists, and are prone to providing misleading second opinions. So we propose a novel fusion attention block network (FABNet) to address these problems. First, we propose a model transfer method based on clinical a priori experience and sample analysis (CPESA) that analyzes the transfer ability by integrating clinical a priori experience using indicators such as the relationship between the cancer onset location and morphology and the texture and staining degree of cell nuclei in histopathology images; our method further validates these indicators by the probability distribution of cancer image samples. Then, we propose a fusion attention block (FAB) structure, which can both provide an advanced non-uniform sparse representation of images and extract spatial relationship information between nuclei; consequently, the LROI can be more accurate and more relevant to pathologists. We conducted extensive experiments, compared with the best Baseline model, the classification accuracy is improved 25%, and It is demonstrated that FABNet performs better on different cancer pathology image datasets and outperforms other state of the art (SOTA) models.
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http://dx.doi.org/10.1109/JBHI.2021.3108999DOI Listing
September 2021

NMN Maintains Intestinal Homeostasis by Regulating the Gut Microbiota.

Front Nutr 2021 29;8:714604. Epub 2021 Jul 29.

School of Medicine, Jiangsu University, Zhenjiang, China.

The aim of this study was to determine the effects of long-term Nicotinamide mononucleotide (NMN) treatment on modulating gut microbiota diversity and composition, as well as its association with intestinal barrier function. In this study, C57BL/6J mice were fed different concentrations of NMN, and their feces were collected for detection of 16S rDNA and non-targeted metabolites to explore the effects of NMN on intestinal microbiota and metabolites. The results revealed that NMN increased the abundance of butyric acid-producing bacteria (Ruminococcae_UCG-014 and Prevotellaceae_NK3B31_group) and other probiotics (Akkermansia muciniphila), while the abundance of several harmful bacteria (Bilophila and Oscillibacter) were decreased after NMN treatment. Meanwhile, the level of bile acid-related metabolites in feces from the G1 group (0.1 mg/ml) was significantly increased compared to the control group, including cholic acid, taurodeoxycholic acid, taurocholic acid, glycocholic acid, and tauro-β-muricholic acid. In addition, long-term NMN treatment affected the permeability of the intestinal mucosa. The number of goblet cells and mucus thickness increased, as well as expression of tight junction protein. These results demonstrate that NMN reduced intestinal mucosal permeability and exerts a protective effect on the intestinal tract. This study lays the foundation for exploring NMN's utility in clinical research.
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http://dx.doi.org/10.3389/fnut.2021.714604DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8358781PMC
July 2021

Effects of Modification Degrees on the Colloidal Stability of Amphiphilic Janus Graphene Oxide in Aqueous Solution with and without Electrolytes.

Langmuir 2021 Aug 15;37(33):10061-10070. Epub 2021 Aug 15.

Shandong Key Laboratory of Oilfield Chemistry, School of Petroleum Engineering, China University of Petroleum (East China), Qingdao 266580, China.

Colloidal stability of modified graphene oxide (GO) is fundamental for its practical applications. Meanwhile, most of the investigations mainly focused on the nanosheets modified by a certain amount of modifiers and neglected the effects of the modification degree, which could vary the physical and chemical properties of modified GO and significantly affect its stability in solution. To the best of our knowledge, this study initially investigated the impact of modification degrees on the colloidal stability of graphene-based amphiphilic Janus nanosheets (JGO) via both experimental and theoretical approaches. The prepared JGO, asymmetrically grafted by dodecylamine, exhibited a direct relation between the modification degree and nanosheet thickness, refractive index, electrostatic properties, hydrophobicity, and the ultimate colloidal stability. In addition, the ionic strength imposed distinctive influences on the aggregation behavior of JGO. Based on the comparison between experimental results and theoretical calculation, it was revealed that the JGO should be modeled as two-dimensional (2D) nanosheets in pure water and be treated as 3D spherical particles in electrolyte solutions for the prediction with the extended Derjaguin-Landau-Verwey-Overbeek theory.
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http://dx.doi.org/10.1021/acs.langmuir.1c01283DOI Listing
August 2021

A Single-Center Clinical Study to Evaluate Shenxiong Glucose Injection Combined with Edaravone in the Treatment of Acute Large-Area Cerebral Infarction.

Biomed Res Int 2021 29;2021:9935752. Epub 2021 Jun 29.

Department of Neurology, The Sichuan Mianyang 404 Hospital, No. 56 Yuejin Road, Mianyang, Sichuan, China.

Objectives: To investigate the clinical efficacy and safety of Shenxiong glucose injection combined with edaravone in the treatment of acute large-area cerebral infarction.

Methods: 156 patients with acute large-area cerebral infarction admitted to our hospital from July 2015 to January 2017 were included in the analysis. The patients were randomly divided into experimental (78 cases) and control (78 cases) groups. Patients in the experimental group were given a 30 mg injection of edaravone in 100 ml of 0.9% sodium chloride solution by intravenous drip, twice a day within 30 minutes and a daily 200 ml injection of Shenxiong glucose by intravenous drip. Patients in the control group were given a 30 mg edaravone injection in 100 ml of 0.9% sodium chloride solution by intravenous drip, twice a day, and the drip was completed within 30 minutes. Patients in both groups were treated for 2 weeks. The levels of fibrinogen (FIB), D-dimer, interleukin 6 (IL-6), P-selectin (CD62P), and hypersensitive C-reactive protein (hs-CRP) were evaluated in the two groups of patients. Neurological disability was evaluated using the modified Rankin scale (mRS) and the neurological deficit score (National Institute of Health Stroke Scale, NIHSS). Adverse reactions to the treatments were also recorded.

Results: No significant differences in age, gender, medical histories, and blood biochemical indices were observed between the two groups before treatment ( > 0.05). After treatment, the levels of FIB, D-dimer, IL-6, CD62P, and hs-CRP were significantly lower following treatment and compared to the control group ( < 0.05). Also, the mRS and NIHSS scores were significantly lower after treatment and compared with the control group ( < 0.05). The total effective rate of the treatment in the experimental group was significantly higher compared to the control group ( < 0.05). During the treatment period, no obvious adverse reactions were observed in the two groups of patients.

Conclusions: In addition to the routine basic treatment of acute large-area cerebral infarction, the addition of Shenxiong glucose injection combined with edaravone injection can improve platelet aggregation and reduce inflammation by affecting P-selectin, D-dimer, and FIB. This treatment approach promotes the recovery of nerve defect function without obvious adverse reactions in patients with acute large-area cerebral infarction.
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http://dx.doi.org/10.1155/2021/9935752DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8263277PMC
September 2021

Dengzhan shengmai capsule combined with donepezil hydrochloride in the treatment of Alzheimer's disease: preliminary findings, randomized and controlled clinical trial.

Rev Assoc Med Bras (1992) 2021 Feb;67(2):190-194

The second People's Hospital of DeYang City, Department of Neurology - DeYang, China.

Objective: To observe the effects of Dengzhan Shengmai capsule combined with donepezil hydrochloride on cognitive function, daily living ability, and safety in patients with Alzheimer's disease.

Methods: A total of 294 patients with Alzheimer's disease were randomly divided into a treatment group and a control group, 147 cases each group. The control group was given oral donepezil hydrochloride 5 mg once a day, and the treatment group was given oral Dengzhan Shengmai capsule 0.36 g three times a day, based on the control group.

Results: At 3 and 6 months of treatment, the ADAS-cog score of the treatment group was 48.69±6.23 and 44.24±5.53; for the control group, 45.48±5.94 and 41.57±5.10. The difference between the two groups is statistically significant (p<0.05). At 3 and 6 months of treatment, the NO level in the treatment group was (46.28±6.68) umol/l, (43.55±7.92) umol/l, and the control group was (42.95±7.92) umol/l, (38.89±5.93) umol/l. The differences between both groups were statistically significant (p<0.05). At 3 and 6 months of treatment, ET levels in the treatment group were (156.08±17.39) ng/l, (144.91±17.60) ng/l, and the control group was (150.48±22.94) ng/l, (135.04±10.08) ng/l. Correlation analysis showed that ADAS-cog score was negatively correlated with NO and ET (p<0.001).

Conclusions: Dengzhan Shengmai capsule combined with donepezil hydrochloride can improve cognitive function and the living capacity of patients with Alzheimer's disease, reduce the production of neurotoxic substances NO and ET, and provide higher safety.
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http://dx.doi.org/10.1590/1806-9282.67.02.20200378DOI Listing
February 2021

Low-protein diets supplemented with glutamic acid or aspartic acid ameliorate intestinal damage in weaned piglets challenged with hydrogen peroxide.

Anim Nutr 2021 Jun 10;7(2):356-364. Epub 2021 Mar 10.

College of Animal Science and Technology, Hunan Agriculture University; Hunan Co-Innovation Center of Animal Production Safety, Changsha, 410128, China.

Glutamic acid (Glu) and aspartic acid (Asp) are acidic amino acids with regulatory roles in nutrition, energy metabolism, and oxidative stress. This study aimed to evaluate the effects of low-protein diets supplemented with Glu and Asp on the intestinal barrier function and energy metabolism in weaned piglets challenged with hydrogen peroxide (HO). Forty piglets were randomly divided into 5 groups: NC, PC, PGA, PG, and PA ( = 8 for each group). Pigs in the NC and PC groups were fed a low-protein diet, while pigs in the PGA, PG, or PA groups were fed the low-protein diet supplemented with 2.0% Glu +1.0% Asp, 2.0% Glu, or 1.0% Asp, respectively. On day 8 and 11, pigs in the NC group were intraperitoneally injected with saline (1 mL/kg BW), while pigs in the other groups were intraperitoneally administered 10% HO (1 mL/kg BW). On day 14, all pigs were sacrificed to collect jejunum and ileum following the blood sample collection in the morning. Notably, low-protein diets supplemented with Glu or Asp ameliorated the intestinal oxidative stress response in HO-challenged piglets by decreasing intestinal expression of genes ( < 0.05) (e.g., manganese superoxide dismutase [], glutathione peroxidase [], and ) encoding oxidative stress-associated proteins, reducing the serum concentration of diamine oxidase ( < 0.05), and inhibiting apoptosis of the intestinal epithelium. Glu and Asp supplementation attenuated the upregulated expression of energy metabolism-associated genes (such as hexokinase and carnitine palmitoyltransferase-1) and the HO-induced activation of acetyl-coenzyme A carboxylase (ACC) in the jejunum and adenosine monophosphate-activated protein kinase-acetyl-ACC signaling in the ileum. Dietary Glu and Asp also ameliorated intestinal barrier damage as indicated by restored intestinal histology and morphology. In conclusion, low-protein diets supplemented with Glu and Asp protected against oxidative stress-induced intestinal dysfunction in piglets, suggesting that this approach could be used as a nutritional regulatory protectant against oxidative stress.
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http://dx.doi.org/10.1016/j.aninu.2020.12.005DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8245806PMC
June 2021

LPCANet: Classification of Laryngeal Cancer Histopathological Images Using a CNN with Position Attention and Channel Attention Mechanisms.

Interdiscip Sci 2021 Dec 17;13(4):666-682. Epub 2021 Jun 17.

Communication Engineering Department, Chongqing College of Electronic Engineering, Chongqing, 401331, China.

Laryngeal cancer is one of the most common malignant tumors in otolaryngology, and histopathological image analysis is the gold standard for the diagnosis of laryngeal cancer. However, pathologists have high subjectivity in their diagnoses, which makes it easy to miss diagnoses and misdiagnose. In addition, according to a literature search, there is currently no computer-aided diagnosis (CAD) algorithm that has been applied to the classification of histopathological images of laryngeal cancer. Convolutional neural networks (CNNs) are widely used in various other cancer classification tasks. However, the potential global and channel relationships of images may be ignored, which will affect the feature representation ability. Simultaneously, due to the lack of interpretability, the results are often difficult to accept by pathologists. we propose a laryngeal cancer classification network (LPCANet) based on a CNN and attention mechanisms. First, the original histopathological images are sequentially cropped into patches. Then, the patches are input into the basic ResNet50 to extract the local features. Then, a position attention module and a channel attention module are added in parallel to capture the spatial dependency and the channel dependency, respectively. The two modules produce the fusion feature map to enhance the feature representation and improve network classification performance. Moreover, the fusion feature map is extracted and visually analyzed by the grad-weighted class activation map (Grad_CAM) to provide a certain interpretability for the final results. The three-class classification performance of LPCANet is better than those of the five state-of-the-art classifiers (VGG16, ResNet50, InceptionV3, Xception and DenseNet121) on the two original resolutions (534 * 400 and 1067 * 800). On the 534 * 400 data, LPCANet achieved 73.18% accuracy, 74.04% precision, 73.15% recall, 72.9% F1-score, and 0.8826 AUC. On the 1067 * 800 data, LPCANet achieved 83.15% accuracy, 83.5% precision, 83.1% recall, 83.1% F1-score, and 0.9487 AUC. The results show that LPCANet enhances the feature representation by capturing the global and channel relationships and achieves better classification performance. In addition, the visual analysis of Grad_CAM makes the results interpretable, which makes it easier for the results to be accepted by pathologists and allows the method to become a second tool for auxiliary diagnosis.
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http://dx.doi.org/10.1007/s12539-021-00452-5DOI Listing
December 2021

Urinary Incontinence Is Associated With Increased All-Cause Mortality in Older Nursing Home Residents: A Meta-Analysis.

J Nurs Scholarsh 2021 Sep 22;53(5):561-567. Epub 2021 May 22.

Lecturer, College of Nursing, JingGangshan Univeristy, Ji'an, Jiangxi, 343009, China.

Purpose: Urinary incontinence is a syndrome common in older adults, but it is not clear whether urinary incontinence is associated with the risk for mortality in elderly nursing home residents.

Methods: We conducted a systematic review and meta-analysis in PubMed, Cochrane, Embase, the Cumulative Index to Nursing and Allied Health Literature (CINAHL), and Web of Science databases. The Newcastle-Ottawa Scale (NOS) was used to assess the quality of the included studies. The meta-analysis was summarized using a random-effects or fixed-effects model, and the heterogeneity among studies was examined using the I2 statistic.

Findings: Six cohort studies with 1,656 participants were included in the final analysis. The NOS score for each study was greater than 6. Urinary incontinence was significantly associated with a higher risk for mortality in nursing homes, with a hazard ratio (HR) of 1.20 (95% confidence interval [CI] 1.12-1.28, I = 41.6%). The significant association of urinary incontinence with increased mortality risk was observed in subgroup analysis according to region, status of dementia, and follow-up period, with a pooled HR of 2.02 (95% CI 1.32-3.11, I = 0%) for Asian countries, 1.18 (95% CI 1.11-1.26, I = 41.6%) for Western countries, 1.17 (95% CI 1.09-1.26, I = 0%) for patients with dementia, 1.35 (95% CI 1.13-1.60, I = 58.9%) for patients without dementia, 1.16 (95% CI 1.07-1.25, I = 43.2%) for studies with a follow-up period of 1 year, and 1.30 (95% CI 1.15-1.48, I = 24.5%) for studies with a follow-up period of more than 1 year.

Conclusions: Urinary incontinence is associated with an increased risk for death among residents of care facilities. Therefore, it was necessary to screen the elderly dwelling in nursing homes who were experiencing or at risk for urinary incontinence with useful tools (e.g., overactive bladder symptom score, bladder control self-assessment questionnaire, three incontinence questions). In addition, early interventions strategies, such as weight loss, stopping smoking, pelvic floor muscle training, and medical and surgical treatments would contribute to decreasing the risk for urinary incontinence and preventing adverse outcomes in nursing home residents.

Clinical Relevance: In our study, we found that the elderly with urinary incontinence who resided in nursing homes had a higher risk for mortality than those without urinary incontinence. Therefore, urinary incontinence in the elderly residing in nursing homes is of particular concern. Early detection and intervention are important for the elderly with urinary incontinence, and caregivers should be made aware of this importance.
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http://dx.doi.org/10.1111/jnu.12671DOI Listing
September 2021

STAT3/SH3PXD2A-AS1/miR-125b/STAT3 positive feedback loop affects psoriasis pathogenesis via regulating human keratinocyte proliferation.

Cytokine 2021 08 13;144:155535. Epub 2021 May 13.

Department of Dermatology, The Second Affiliated Hospital of Hunan University of Chinese Medicine, Changsha, Hunan Province 410005, China.

Psoriasis is a chronic immune-mediated inflammatory dermatosis. STAT3 has been considered a critical regulator of psoriasis pathogenesis due to its role in inflammation and immune responses. Furthermore, alongside non-coding RNAs, including long non-coding RNAs (lncRNAs) and miRNAs, STAT3 also plays a critical role in psoriasis pathogenesis. Two sets of online microarray profiles (GSE50790 and GSE13355) were subsequently downloaded and analyzed to search for lncRNAs upregulated in psoriasis lesion tissues. The expression of lncRNA SH3PXD2A-AS1 could be remarkably upregulated in psoriasis specimens. SH3PXD2A-AS1 silence was found to suppress HaCaT cell proliferation and promote HaCaT cell apoptosis significantly. Meanwhile, SH3PXD2A-AS1 silence significantly increased cleaved-caspase-3 protein levels and inhibited S100A7, TNF-α, IL-6, p-STAT3, STAT3, CyclinD1, and survivin protein levels. Moreover, the expression of miR-125b could be substantially decreased within psoriasis lesion tissue samples, while miR-125b could negatively regulate the SH3PXD2A-AS1 and STAT3 expression. As predicted by an online tool and validated by luciferase reporter and RIP assays, miR-125b was found to bind to SH3PXD2A-AS1 and STAT3 3'UTR directly; SH3PXD2A-AS1 competed with 3'UTR of STAT3 for miR-125b binding to counteract miR-125b-mediated suppression of STAT3. STAT3 is known to activate the transcription of SH3PXD2A-AS1 through the targeting of its promoter region. It consequentially forms a regulatory feedback loop promoting SH3PXD2A-AS1 expression affecting HaCat cell proliferation and apoptosis. A novel STAT3 related mechanism whereby STAT 3/ SH3PXD2A-AS1/ miR-125b/STAT3 positive feedback loop which could potentially affect the pathogenesis of Psoriasis has been established.
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http://dx.doi.org/10.1016/j.cyto.2021.155535DOI Listing
August 2021

The Relationship between Serum Amyloid A Level and Cognitive Dysfunction in Patients with Vascular Dementia: Preliminary Findings.

Biomed Res Int 2021 15;2021:6676144. Epub 2021 Feb 15.

Department of Neurology, People's Hospital of Deyang City, No. 173 TaiShan North Road, Deyang, Sichuan 618000, China.

Objective: This study was aimed at investigating the relationship between serum amyloid A (SAA) levels and cognitive dysfunction in patients with vascular dementia (VAD).

Methods: Using cross-sectional research methods, 146 patients with VAD were selected as the VAD group and 70 normal people were selected as the NC group. Upon admission, the clinical and biochemical characteristics of the two groups of study subjects were collected, and the MMSE scale was used to assess cognitive function. A sandwich enzyme-linked immunosorbent assay was used to detect SAA levels.

Results: There was no significant difference in clinical data and biochemical characteristics in the VAD group ( > 0.05). Compared with the VAD group, the NC group has a higher level of education ( < 0.05). The SAA level of the VAD group was higher than that of the NC group, and there was a significant difference ( < 0.05). Spearman correlation analysis showed that SAA and MMSE in the VAD group were negatively correlated. Further multiple regression analysis showed that the serum amyloid A level is an independent risk factor for cognitive dysfunction in VAD patients.

Conclusion: The level of SAA in VAD patients is significantly increased, which can be used as a potential peripheral blood marker to predict cognitive impairment in VAD patients.
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http://dx.doi.org/10.1155/2021/6676144DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7902129PMC
May 2021

Expression and Significance of MicroRNA-126 and MicroRNA-21 in Peripheral Blood Mononuclear Cells in Patients with Myasthenia Gravis.

Neuroimmunomodulation 2021 Feb 3:1-8. Epub 2021 Feb 3.

Department of Neurology, The Second People's Hospital of Deyang City, DeYang, China,

Objective: The aim of the study was to investigate the expression and significance of microRNA-126, microRNA-21, FOXP3mRNA, acetylcholine receptor antibody (AChR-Ab), and interleukin 6 (IL-6) in peripheral blood of patients with myasthenia gravis (MG).

Methods: From September 2015 to March 2018, 60 patients with MG who were first diagnosed as MG were selected as the MG group, and 50 healthy people in the same period were selected as the normal group. RT-PCR technology was used to detect the relative expression of microRNA-126, microRNA-21, and FOXP3mRNA in peripheral blood mononuclear cells of the MG group and the control group. The EILISA and IPA technique was used to detect the expression levels of IL-6 and AChR-Ab in the peripheral serum of the 2 groups.

Results: There were no differences between groups regarding patient's characteristics and baseline data. microRNA-21 and microRNA-126 are highly conserved in the human genome. microRNA-21, microRNA-126, FOXP3mRNA, AChR-Ab, and IL-6 are differentially expressed in the MG group and the control group (p < 0.05). microRNA-21 is positively correlated with AChR-Ab and IL-6 in MG patients (r = 0.746, 0.789, p < 0.05), but has no correlation with FOXP3mRNA (r = -0.249 p = 0.055), while micro-RNA-126 is positively correlated with FOXP3mRNA and negatively correlated with AChR-Ab and IL-6 (r = 0.526, -0.797, -0.801, p < 0.05).

Conclusion: Patients with MG have differential expression of microRNA-21 and microRNA-126 and may participate in the pathogenesis of MG by regulating pathways related to inflammatory immune response.
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http://dx.doi.org/10.1159/000510714DOI Listing
February 2021

The Value of Combined Detection of D-dimer and CD62p in Judging the Severity of Acute Cerebral Infarction and Short-Term Prognosis.

Biomed Res Int 2021 14;2021:6620311. Epub 2021 Jan 14.

Department of Neurology, People's Hospital of Deyang City, No. 173 TaiShan North Road, Deyang, Sichuan 618000, China.

Objective: To explore the value of combined detection of peripheral blood P-selectin (CD62p) and D-dimer (D-dimer) in the judgment of acute cerebral infarction severity and short-term prognosis.

Methods: 268 patients with acute cerebral infarction from February 2015 to February 2019 were selected as the observation group. According to the National Institute of Health stroke scale, there were 90 cases (SCI group), 88 cases (MOCI group), and 90cases (MICI group) in the severe, moderate, and mild cerebral infarction groups, respectively. In the same period, 80 cases of healthy people served as the Normal group. Use flow cytometry to detect CD62p in peripheral blood and magnetic bead method to detect D-dimer level within 24 hours of onset. Logistic regression was used to analyze whether the two are factors affecting the short-term prognosis of acute cerebral infarction, and the ROC curve was drawn to evaluate the value of the combined detection of the two in the short-term prognosis of patients with acute cerebral infarction.

Results: Peripheral blood D-dimer and CD62p levels (2.95 ± 0.76 ng/l, 34.03 ± 5.29 ng/l) in the SCI group were higher than those in the MOCI group (2.30 ± 0.51 ng/l, 27.58 ± 5.56 ng/l) and the MICI group (1.87 ± 0.40 ng/l, 19.60 ± 3.98 ng/l); the difference between the groups was statistically significant ( < 0.05). Logistic regression analysis showed that D-dimer and CD62p were independent risk factors affecting the poor prognosis of patients with acute cerebral infarction (OR values were 3.752 and 1.213, and 95% CI were 1.612-7.934 and 1.093-1.342, respectively, both < 0.05). The AUC of D-dimer combined with CD62p for predicting poor prognosis of acute cerebral infarction is 0.859, which is better than D-dimer and CD62p alone.

Conclusion: Peripheral blood D-dimer combined with CD62p detection is helpful for the risk stratification and short-term prognosis assessment of patients with acute cerebral infarction. Clinical detection is of great significance for the prevention and monitoring of disease development.
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http://dx.doi.org/10.1155/2021/6620311DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7834744PMC
May 2021

The Relationship between the Mean Platelet Volume and Carotid Atherosclerosis and Prognosis in Patients with Acute Cerebral Infarction.

Biomed Res Int 2020 30;2020:6685740. Epub 2020 Dec 30.

Department of Neurology, People's Hospital of Deyang City, No. 173 TaiShan North Road, Deyang, Sichuan, China.

Objective: To investigate the relationship between mean platelet volume (MPV) level and carotid atherosclerosis and prognosis in patients with acute cerebral infarction. A retrospectively included 160 patients with acute cerebral infarction classified by TOAST classification as aortic atherosclerosis as the observation group. To analyze the relationship between MPV and carotid atherosclerosis, and use receiver operating characteristic (ROC) curves to analyze the role of MPV in predicting the prognosis of acute cerebral infarction in the observation group, grouping patients with different MPV by cut-off value, and analyze the differences in factors between the two groups of patients.

Results: MPV has a positive correlation with carotid atherosclerosis in patients with acute cerebral infarction. Multivariate logistic regression analysis revealed that increased MPV was an independent predictor of poor functional outcome in patients with acute cerebral infarction (Odds Ratio (OR): 6.152, 95% CI: 2.385-13.625, < 0.01). ROC curve analysis showed that the area under the curve for MPV to predict poor prognosis was 0.868 (95% CI: 0.787-949, < 0.01). The cutoff value, sensitivity, and specificity were 12.65, 76.2%, and 87.6%. Compared with patients with MPV < 12.65 at admission, patients with higher MPV levels (MPV ≥ 12.65) at admission have larger infarct size, more severe carotid artery stenosis, poor short-term prognosis, and higher mortality.

Conclusion: MPV level is closely related to the degree of carotid atherosclerosis in patients with acute cerebral infarction, and it is also an independent predictor of poor prognosis in patients with acute cerebral infarction at 3 months.
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http://dx.doi.org/10.1155/2020/6685740DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7790567PMC
May 2021

CSN6 promotes melanoma proliferation and metastasis by controlling the UBR5-mediated ubiquitination and degradation of CDK9.

Cell Death Dis 2021 01 22;12(1):118. Epub 2021 Jan 22.

State Key Laboratory of Silkworm Genome Biology, Southwest University, 400715, Chongqing, China.

As a critical subunit of the constitutive photomorphogenesis 9 (COP9) signalosome (CSN), CSN6 is upregulated in some human cancers and plays critical roles in tumorigenesis and progression, but its biological functions and molecular mechanisms in melanoma remain unknown. Our study showed that CSN6 expression was upregulated in melanoma patients and cells, and correlated with poor survival in melanoma patients. In melanoma cells, CSN6 knockdown remarkably inhibited cell proliferation, tumorigenicity, migration, and invasion, whereas CSN6 recovery rescued the proliferative and metastatic abilities. Notably, we identified that CSN6 stabilized CDK9 expression by reducing CDK9 ubiquitination levels, thereby activating CDK9-mediated signaling pathways. In addition, our study described a novel CSN6-interacting E3 ligase UBR5, which was negatively regulated by CSN6 and could regulate the ubiquitination and degradation of CDK9 in melanoma cells. Furthermore, in CSN6-knockdown melanoma cells, UBR5 knockdown abrogated the effects caused by CSN6 silencing, suggesting that CSN6 activates the UBR5/CDK9 pathway to promote melanoma cell proliferation and metastasis. Thus, this study illustrates the mechanism by which the CSN6-UBR5-CDK9 axis promotes melanoma development, and demonstrate that CSN6 may be a potential biomarker and anticancer target in melanoma.
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http://dx.doi.org/10.1038/s41419-021-03398-0DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7822921PMC
January 2021

Effect of Argatroban Injection on Clinical Efficacy in Patients with Acute Cerebral Infarction: Preliminary Findings.

Eur Neurol 2021 21;84(1):38-42. Epub 2021 Jan 21.

Department of Neurology, The Second People's Hospital of Deyang City, DeYang, China,

Objective: The aim is to observe the effects of argatroban injection and butylphthalide injection on blood flow rheology, clinical efficacy, and safety in patients with acute cerebral infarction.

Methods: 344 patients with acute cerebral infarction within 48 h after admission were divided into treatment group and control group, with 172 cases in each group. The control group received routine treatment. The treatment group received argatroban injection 60 mg on the basis of the control group, intravenously guttae (ivgtt) was used for 2 days and then changed to argatroban injection 10 mg, ivgtt bid for 5 days, and the total course of treatment was 7 days. The neurological changes, activities of daily living, and the rheology indicators (fibrinogen [Fib], platelet aggregation rate [Pag], whole blood high shear viscosity [Whsv], hematocrit [Hct]) were compared between the 2 groups, clinical efficacy and adverse drug reactions.

Results: After treatment, the total effective rates of the treatment group and the control group were 90.70% (156 /172 cases) and 74.41% (128 and 172 cases), respectively, and the difference was statistically significant (p < 0.05). After treatment, the National Institutes of Health Stroke Scale scores of the treatment group and the control group were (7.05 ± 1.97) and (8.30 ± 1.79), respectively, and the Barthel index was (68.02 ± 11.07) and (62.32 ± 11.46), respectively. The difference was statistically significant (p < 0.05). After treatment, the treatment group and the control group were (2.66 ± 0.22) g/L and (3.50 ± 0.22) g/L, respectively, and Pag were (0.68 ± 0.06)% and (0.81 ± 0.09)%, respectively, and Whsv was (6.44 ± 0.76) mPs/s and (6.87 ± 0.91) mPs/s, Hct were (8.19 ± 1.21)% and (10.44 ± 1.04)%, respectively, and the differences were statistically significant (p < 0.05). The incidence of adverse reactions in the treatment group and the control group was 6.97 and 5.81%, respectively, and the difference was not statistically significant (p > 0.05).

Conclusion: Argatroban injection is effective in the treatment of acute cerebral infarction, which can significantly improve the hemorheology of patients with good safety.
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http://dx.doi.org/10.1159/000512813DOI Listing
November 2021

Weighted gene coexpression network and experimental analyses identify lncRNA SPRR2C as a regulator of the IL-22-stimulated HaCaT cell phenotype through the miR-330/STAT1/S100A7 axis.

Cell Death Dis 2021 01 15;12(1):86. Epub 2021 Jan 15.

Department of Dermatology, the Second Affiliated Hospital, The Domestic First-class Discipline Construction Project of Chinese Medicine of Hunan University of Chinese Medicine, Changsha, 410005, Hunan, China.

Psoriasis is a chronic inflammatory disease of the skin with highly complex pathogenesis. In this study, we identified lncRNA SPRR2C (small proline-rich protein 2C) as a hub gene with a critical effect on the pathogenesis of psoriasis and response to treatment using both weighted gene coexpression network analysis (WGCNA) and differential expression analysis. SPRR2C expression was significantly upregulated in both psoriatic lesion samples and HaCaT cell lines in response to IL-22 treatment. After SPRR2C knockdown, IL-22-induced suppression of HaCaT proliferation, changes in the KRT5/14/1/10 protein levels, and suppression of the IL-1β, IL-6, and TNF-α mRNA levels were dramatically reversed. In the coexpression network with SPRR2C based on GSE114286, miR-330 was significantly negatively correlated with SPRR2C, while STAT1 and S100A7 were positively correlated with SPRR2C. By binding to miR-330, SPRR2C competed with STAT1 and S100A7 to counteract miR-330-mediated suppression of STAT1 and S100A7. MiR-330 overexpression also reversed the IL-22-induced changes in HaCaT cell lines; in response to IL-22 treatment, miR-330 inhibition significantly attenuated the effects of SPRR2C knockdown. STAT1 and S100A7 expression was significantly upregulated in psoriatic lesion samples. The expression of miR-330 had a negative correlation with the expression of SPRR2C, while the expression of SPRR2C had a positive correlation with the expression of STAT1 and S100A7. Thus, SPRR2C modulates the IL-22-stimulated HaCaT cell phenotype through the miR-330/STAT1/S100A7 axis. WGCNA might uncover additional biological pathways that are crucial in the pathogenesis and response to the treatment of psoriasis.
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http://dx.doi.org/10.1038/s41419-020-03305-zDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7810847PMC
January 2021

The Roles of Integrin α5β1 in Human Cancer.

Onco Targets Ther 2020 31;13:13329-13344. Epub 2020 Dec 31.

State Key Laboratory of Silkworm Genome Biology, Key Laboratory for Sericulture Biology and Genetic Breeding, Ministry of Agriculture and Rural Affairs, Southwest University, Chongqing 400716, People's Republic of China.

Cell adhesion to the extracellular matrix has important roles in tissue integrity and human health. Integrins are heterodimeric cell surface receptors that are composed by two non-covalently linked alpha and beta subunits that mainly participate in the interaction of cell-cell adhesion and cell-extracellular matrix and regulate cell motility, adhesion, differentiation, migration, proliferation, etc. In mammals, there have been eighteen α subunits and 8 β subunits and so far 24 distinct types of αβ integrin heterodimers have been identified in humans. Integrin α5β1, also known as the fibronectin receptor, is a heterodimer with α5 and β1 subunits and has emerged as an essential mediator in many human carcinomas. Integrin α5β1 alteration is closely linked to the progression of several types of human cancers, including cell proliferation, angiogenesis, tumor metastasis, and cancerogenesis. In this review, we will introduce the functions of integrin α5β1 in cancer progression and also explore its regulatory mechanisms. Additionally, the potential clinical applications as a target for cancer imaging and therapy are discussed. Collectively, the information reviewed here may increase the understanding of integrin α5β1 as a potential therapeutic target for cancer.
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http://dx.doi.org/10.2147/OTT.S273803DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7781020PMC
December 2020

Bruceine D inhibits Cell Proliferation Through Downregulating LINC01667/MicroRNA-138-5p/Cyclin E1 Axis in Gastric Cancer.

Front Pharmacol 2020 24;11:584960. Epub 2020 Nov 24.

State Key Laboratory of Silkworm Genome Biology, Institute of Sericulture and Systems Biology, College of Sericulture and Textile and Biomass Science, Southwest University, Chongqing, China.

Gastric cancer is one of the most common malignant tumors. Bruceine D (BD) is one of the extracts of . In recent years, it has been reported that BD has anti-tumor activity in some human cancers through different mechanisms. Here, this study try to explore the effect of BD on gastric cancer and its regulatory mechanism. Cell proliferation ability was detected by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) assays, 5-bromo-2-deoxyuridine (BrdU) staining and soft agar colony formation assay, respectively. The tumor xenograft model was used to verify the effect of BD on the tumorigenicity of gastric cancer cells . Flow cytometry analysis and Western blot assay were performed to detect cell cycle and apoptosis. Gastric cancer cells were analyzed by transcriptome sequencing. The interaction between LINC01667, microRNA-138-5p (miR-138-5p) and Cyclin E1 was verified by dual luciferase experiment and RT-PCR assays. We found that BD significantly inhibited cell proliferation and induced cell cycle arrest at S phase in gastric cancer cells. Transcriptome analysis found that the expression of a long non-coding RNA, LINC01667, were significantly down-regulated after BD treatment. Mechanically, it was discovered that LINC01667 upregulated the expression of Cyclin E1 by sponging miR-138-5p. Furthermore, BD enhanced the chemosensitivity of gastric cancer cells to doxorubicin, a clinically used anti-cancer agent. BD inhibit the growth of gastric cancer cells by downregulating the LINC01667/miR-138-5p/Cyclin E1 axis. In addition, BD enhances the chemosensitivity of gastric cancer cells to doxorubicin. This study indicates that BD may be used as a candidate drug for the treatment of patients with gastric cancer.
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http://dx.doi.org/10.3389/fphar.2020.584960DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7774499PMC
November 2020

Effects of Carotid Artery Stent and Carotid Endarterectomy on Cognitive Function in Patients with Carotid Stenosis.

Biomed Res Int 2020 16;2020:6634537. Epub 2020 Dec 16.

Department of Neurology, The Second People's Hospital of Deyang City, No. 340 Minjiang West Road, DeYang, Sichuan 618000, China.

Background: Carotid artery stenosis is closely related to cognitive dysfunction, in which decreased cerebral perfusion is one of the important factors. Both carotid artery stent implantation and carotid endarterectomy can relieve stenosis and increase cerebral perfusion. In this study, we aimed to compare the effects of carotid artery stent implantation and endarterectomy on cognitive function.

Methods: A total of 98 patients with carotid artery stenosis hospitalized in our hospital from July 2015 to January 2017 were included. Among them, 50 cases underwent carotid artery stent implantation treatment as stent implantation group (CAS group), and 48 cases underwent carotid artery endarterectomy treatment as carotid endarterectomy group (CEA group). Using the Mini-Mental State Examination Scale (MMSE Scale) and the Montreal Cognitive Assessment Scale (MoCA Scale), the cognitive function scores of the two groups of patients before and after 3 and 6 months of operation were measured, and the patients were also measured before and after surgery, after the serum NSE, hs-CRP content.

Results: The serum NSE, hs-CRP content, MMSE score, and MoCA score of the two groups before treatment were not statistically significant ( > 0.05). The MMSE score and MoCA score of the two groups of patients before treatment were lower than the normal value, suggesting carotid artery stenosis combined with different degrees of cognitive dysfunction. Carotid artery stenosis is different, and patients' cognitive function is also different. The MMSE score and MoCA score of the two groups at 3 and 6 months after operation were higher than before treatment, and there was a statistically significant difference between 6 and 3 months after operation ( < 0.05), but at each time There was no statistically significant difference between the two groups ( > 0.05). The NSE content of the two groups of patients after operation decreased compared with that before treatment, and the decrease in 6 months after operation was more obvious than that in March ( < 0.05). However, the difference between the two groups at each time point was not statistically significant ( > 0.05). The content of hs-CRP in the two groups of patients was higher than that before the operation, and the CAS group was significantly higher than the CEA group; the difference was statistically significant ( < 0.05).

Conclusion: Carotid artery stent and carotid endarterectomy are effective in improving the cognitive function of patients with carotid stenosis, but there is no significant difference between the two.
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http://dx.doi.org/10.1155/2020/6634537DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7762647PMC
June 2021

The Role of miRNA-146a and Proinflammatory Cytokines in Carotid Atherosclerosis.

Biomed Res Int 2020 9;2020:6657734. Epub 2020 Dec 9.

Department of Neurology, The Second People's Hospital of Deyang City, 340 Minjiang West Road, Deyang, Sichuan 618000, China.

The aim of this study was to investigate the expression and significance of miRNA-146a in peripheral blood of patients with carotid atherosclerosis (CAS). Patients with CAS were selected as the stenosis (CAS) group ( = 180). According to the degree of stenosis, they were divided into the mild stenosis group (MS group, = 64), moderate stenosis group (M group, = 62 cases), and severe stenosis group (SS group, = 54). According to the plaque type, patients were divided into a stable plaque group (SP group, = 96) and a vulnerable plaque group (VP group, = 84). Patients without CAS represented the normal group ( = 90). The expression levels of miRNA-146a as well as IL-6 and TNF- in peripheral blood were detected by RT-PCR and ELISA, respectively. The expression levels of miRNA-146a, IL-6, and TNF- in the CAS group were higher than those in the normal group and positively correlated with the degree of stenosis and plaque vulnerability. The expression levels of miRNA-146a, IL-6, and TNF- in the stable plaque group were lower than those in the vulnerable plaque group. The area under the curve of miRNA-146a predicting the vulnerability of plaques was significant at 0.641. The expression level of miRNA-146a in the CAS group was positively correlated with IL-6 and TNF- levels. Our results indicate that miRNA-146a may participate in the occurrence and development of CAS by regulating the expression of IL-6 and TNF-, which may be potential biomarkers of CAS.
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http://dx.doi.org/10.1155/2020/6657734DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7746461PMC
June 2021

AF-SENet: Classification of Cancer in Cervical Tissue Pathological Images Based on Fusing Deep Convolution Features.

Sensors (Basel) 2020 Dec 27;21(1). Epub 2020 Dec 27.

School of Microelectronics and Communication Engineering, Chongqing University, Chongqing 400044, China.

Cervical cancer is the fourth most common cancer in the world. Whole-slide images (WSIs) are an important standard for the diagnosis of cervical cancer. Missed diagnoses and misdiagnoses often occur due to the high similarity in pathological cervical images, the large number of readings, the long reading time, and the insufficient experience levels of pathologists. Existing models have insufficient feature extraction and representation capabilities, and they suffer from insufficient pathological classification. Therefore, this work first designs an image processing algorithm for data augmentation. Second, the deep convolutional features are extracted by fine-tuning pre-trained deep network models, including ResNet50 v2, DenseNet121, Inception v3, VGGNet19, and Inception-ResNet, and then local binary patterns and a histogram of the oriented gradient to extract traditional image features are used. Third, the features extracted by the fine-tuned models are serially fused according to the feature representation ability parameters and the accuracy of multiple experiments proposed in this paper, and spectral embedding is used for dimension reduction. Finally, the fused features are inputted into the Analysis of Variance-F value-Spectral Embedding Net (AF-SENet) for classification. There are four different pathological images of the dataset: normal, low-grade squamous intraepithelial lesion (LSIL), high-grade squamous intraepithelial lesion (HSIL), and cancer. The dataset is divided into a training set (90%) and a test set (10%). The serial fusion effect of the deep features extracted by Resnet50v2 and DenseNet121 () is the best, with average classification accuracy reaching 95.33%, which is 1.07% higher than ResNet50 v2 and 1.05% higher than DenseNet121. The recognition ability is significantly improved, especially in LSIL, reaching 90.89%, which is 2.88% higher than ResNet50 v2 and 2.1% higher than DenseNet121. Thus, this method significantly improves the accuracy and generalization ability of pathological cervical WSI recognition by fusing deep features.
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http://dx.doi.org/10.3390/s21010122DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7795214PMC
December 2020
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