Publications by authors named "Huan Yu"

436 Publications

Short Sleep Duration as a Risk Factor for Depression, Anxiety and Fatigue in Patients with Leukemia.

Neuropsychiatr Dis Treat 2022 29;18:1573-1582. Epub 2022 Jul 29.

Department of Hematology, Chenzhou First People's Hospital, Chenzhou City, Hunan Province, People's Republic of China.

Objective: Our study aimed to investigate the level of depression, anxiety, and fatigue in patients with leukemias, and analyze the influence of sleep duration on the mental symptoms of patients with leukemias.

Methods: A total of 127 patients were enrolled in our study and completed the questionnaire survey. Self-Assessment Depression Scale (SDS), Self-Assessment Anxiety Scale (SAS), and the Fatigue Scale-14 (FS-14) were adopted. The patient's lifestyle information, including exercise, alcohol abuse, and smoking, was obtained from the patient's self-report.

Results: Depression score of patients with sleep duration >8 hours (long duration group) was 56.21±11.63, which was significantly lower than that of patients sleep duration between 6 and 8 hours (medium duration group) with 59.61±8.77 and patients sleep <6 hours (short duration group) with 64.82±6.42 (P = 0.007). Similarly, the anxiety score of long duration group, medium duration group and short duration group was 45.36±11.41, 48.26±6.96 and 53.53±5.87, respectively (P = 0.005). The fatigue score of short duration group is 8.47±2.45, which is higher than others (P = 0.046). To further identify the relationship, we evaluated physical fatigue and mental fatigue levels in patients with or without symptoms of depression. We found that patients with symptoms of depression have a higher score both in physical fatigue and mental fatigue (all p < 0.001). Similar trend was observed in patients with or without symptoms of anxiety (all p < 0.001). However, alzhough patients with leukemia in long duration group have the highest score of physical fatigue (p = 0.016), no significant difference in mental fatigue was found in different sleeping duration group (p = 0.587). Furthermore, multivariate analyses were conducted and revealed that sleep duration was the independent factor associated with depression (OR = 0.270, P = 0.003) and anxiety (OR = 0.473, P = 0.010).

Conclusion: For leukemia patients with short sleep duration, a prompt evaluation of their level of depression, anxiety, and fatigue and the initiation of timely interventions is essential.
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http://dx.doi.org/10.2147/NDT.S362229DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9346604PMC
July 2022

Alterations of Plasma Lipids in Adult Women With Major Depressive Disorder and Bipolar Depression.

Front Psychiatry 2022 18;13:927817. Epub 2022 Jul 18.

Department of Psychiatry, Chang'an Hospital, Xi'an, China.

Lipidomics has been established as a potential tool for the investigation of mental diseases. However, the composition analysis and the comparison of the peripheral lipids regarding adult women with major depressive depression (MDD) or bipolar depression (BPD) has been poorly addressed. In the present study, age-matched female individuals with MDD ( = 28), BPD ( = 22) and healthy controls (HC, = 25) were enrolled. Clinical symptoms were assessed and the plasma samples were analyzed by comprehensive lipid profiling based on liquid chromatography-mass spectrometry (LC/MS). We found that the composition of lipids was remarkably changed in the patients with MDD and BPD when compared to HC or compared to each other. Moreover, we identified diagnostic potential biomarkers comprising 20 lipids that can distinguish MDD from HC (area under the curve, AUC = 0.897) and 8 lipids that can distinguish BPD from HC (AUC = 0.784), as well as 13 lipids were identified to distinguish MDD from BPD with moderate reliability (AUC = 0.860). This study provides further understanding of abnormal lipid metabolism in adult women with MDD and BPD and may develop lipid classifiers able to effectively discriminate MDD from BPD and HC.
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http://dx.doi.org/10.3389/fpsyt.2022.927817DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9339614PMC
July 2022

Assessment of the Potential of Nakai. in Treating Ethanol-Induced Gastric Ulcer in Rats Based on Metabolomics and Network Analysis.

Front Pharmacol 2022 12;13:810344. Epub 2022 Jul 12.

School of Pharmacy, Jiangxi University of Chinese Medicine, Nanchang, China.

Gastric ulcer (GU) is one of the most commonly diagnosed diseases worldwide, threatening human health and seriously affecting quality of life. Reports have shown that the Chinese herbal medicine (Thunb.) Nakai (SGN) can treat GU. However, its pharmacological effects deserve further validation; in addition, its mechanism of action is unclear. An acute gastric ulcer (AGU) rat model induced by alcohol was used to evaluate the gastroprotective effect of SGN by analysis of the histopathological changes in stomach tissue and related cytokine levels; the potential mechanisms of action of SGN were investigated via serum metabolomics and network pharmacology. Differential metabolites of rat serum were identified by metabolomics and the metabolic pathways of the identified metabolites were enriched via MetaboAnalyst. Furthermore, the critical ingredients and candidate targets of SGN anti-AGU were elucidated. A compound-reaction-enzyme-gene network was established using Cytoscape version 3.8.2 based on integrated analysis of metabolomics and network pharmacology. Finally, molecular docking was applied to verify the acquired key targets. The results showed that SGN exerted a certain gastroprotective effect via multiple pathways and targets. The effects of SGN were mainly caused by the key active ingredients isofraxidin, rosmarinic, and caffeic acid, which regulate hub targets, such as PTGS2, MAPK1, and KDR, which maintain the homeostasis of related metabolites. Signal pathways involved energy metabolism as well as immune and amino acid metabolism. Overall, the multi-omics techniques were proven to be promising tools in illuminating the mechanism of action of SGN in protecting against diseases. This integrated strategy provides a basis for further research and clinical application of SGN.
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http://dx.doi.org/10.3389/fphar.2022.810344DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9315220PMC
July 2022

Phenotypic modulation of vascular smooth muscle cells in the corpus spongiosum surrounding the urethral plate in hypospadias.

Andrologia 2022 Jul 22:e14540. Epub 2022 Jul 22.

Department of Urology, Shanghai Children's Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai, China.

Hypospadias is an abnormal ventral development of the penis caused by incomplete virilization of the male genital tubercle. This study investigated the phenotypic modulation of vascular smooth muscle cells (VSMCs) in the corpus spongiosum surrounding the urethral plate in hypospadias. The urethral corpus spongiosum tissue was collected for HE, Masson and α-SMA immunohistochemical staining. Spongiosum VSMCs were cultured and identified by α-SMA fluorescence. qRT-PCR and Western blotting and fluorescence were performed. The results showed that the vascular lumen of the corpus spongiosum around the urethral plate was larger and that the vascular smooth muscle layer was thicker in hypospadias. The expression of the contractile markers α-SMA and Calponin 1 in VSMCs was decreased, the expression of the synthetic marker OPN was increased, and the transcription of the phenotypic switching factors SRF and MYOCD was decreased. The expression of Ki67, PCNA and BAX was increased, and the expression of Bcl-2 was decreased. The phenotype of corpus spongiosum VSMCs in hypospadias changed from the contractional type to the synthetic type. This phenotypic modulation was associated with increased proliferation and apoptosis rates. SRF and MYOCD may be the main factors mediating the phenotypic modulation of urethral corpus spongiosum VSMCs.
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http://dx.doi.org/10.1111/and.14540DOI Listing
July 2022

Consistent Abnormalities in Metabolic Patterns of Lewy Body Dementias.

Mov Disord 2022 Jul 20. Epub 2022 Jul 20.

PET Center, Huashan Hospital, Fudan University, Shanghai, China.

Background: Whether dementia with Lewy bodies (DLB) and Parkinson's disease (PD) dementia (PDD) represent the same disease, distinct entities, or conditions within the same spectrum remains controversial.

Objective: The objective of this study was to provide new insight into this debate by separately identifying disease-specific metabolic patterns and comparing them with each other and with previously established PD-related pattern (PDRP).

Methods: Patients with DLB (n = 67), patients with PDD (n = 50), and healthy control subjects (HCs; n = 15) with brain F-fluorodeoxyglucose positron emission tomography were enrolled as cohorts A and B for pattern identification and validation, respectively. Patients with PD (n = 30) were included for discrimination. Twenty-one participants had two scans. The principal component analysis was applied for pattern identification (DLB-related pattern [DLBRP], PDD-related pattern [PDDRP]). Similarities and differences among three patterns were assessed by pattern topography, pattern expression, clinical correlations cross-sectionally, and pattern expression changes longitudinally.

Results: DLBRP and PDDRP shared highly similar topographies, with relative hypometabolism mainly in the middle temporal gyrus, middle occipital gyrus, lingual gyrus, precuneus, cuneus, angular gyrus, superior and inferior parietal gyrus, middle and inferior frontal gyrus, cingulate, and caudate, and relative hypermetabolism in the cerebellum, putamen, thalamus, precentral/postcentral gyrus, and paracentral lobule, which were more extensive than the PDRP. Patients with DLB and PDD could not be distinguished successfully by any pattern, but patients with PD were easily recognized, especially by DLBRP and PDDRP. The pattern expression of DLBRP and PDDRP showed similar efficacy in cross-sectional disease severity assessment and longitudinal progression monitoring.

Conclusions: The consistent abnormalities in metabolic patterns of DLB and PDD might underline the potential continuum across the clinical spectrum from PD to DLB. © 2022 International Parkinson and Movement Disorder Society.
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http://dx.doi.org/10.1002/mds.29138DOI Listing
July 2022

Engineering whole-cell microbial biosensors: Design principles and applications in monitoring and treatment of heavy metals and organic pollutants.

Biotechnol Adv 2022 Jul 16;60:108019. Epub 2022 Jul 16.

Frontiers Science Center for Synthetic Biology (Ministry of Education), Key Laboratory of Systems Bioengineering, Tianjin University, Tianjin 300072, China; Collaborative Innovation Center of Chemical Science and Engineering, School of Chemical Engineering and Technology, Tianjin University, Tianjin 300072, China. Electronic address:

Biosensors have been widely used as cost-effective, rapid, in situ, and real-time analytical tools for monitoring environments. The development of synthetic biology has enabled emergence of genetically engineered whole-cell microbial biosensors. This review updates the design and optimization principles for a diverse array of whole-cell biosensors based on transcription factors (TF) including activators or repressors derived from heavy metal resistance systems, alkanes, and aromatics metabolic pathways of bacteria. By designing genetic circuits, the whole-cell biosensors could be engineered to intelligently sense heavy metals (Hg, Zn, Pb, Au, Cd, As, Ni, Cu, and UO) or organic compounds (alcohols, alkanes, phenols, and benzenes) through one-component or two-component system-based TFs, transduce signals through genetic amplifiers, and response as various outputs such as cell fluorescence and bioelectricity for monitoring heavy metals and organic pollutants in real conditions, synthetic curli and surface metal-binding peptides for in situ bio-sorption of heavy metals. We further review strategies that have been implemented to optimize the selectivity and correlation between ligand concentration and output signal of the TF-based biosensors, so as to meet requirements of practical applications. The optimization strategies include protein engineering to change specificities, promoter engineering to improve sensitivities, and genetic circuit-based amplification to enhance dynamic ranges via designing transcriptional amplifiers, logic gates, and feedback loops. At last, we outlook future trends in developing novel forms of biosensors.
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http://dx.doi.org/10.1016/j.biotechadv.2022.108019DOI Listing
July 2022

Association between Dietary Patterns Reflecting C-Reactive Protein and Metabolic Syndrome in the Chinese Population.

Nutrients 2022 Jun 21;14(13). Epub 2022 Jun 21.

Department of Epidemiology and Biostatistics, School of Public Health, Peking University Health Science Center, Beijing 100191, China.

It is unclear how the dietary patterns reflecting C-reactive protein (CRP) affect metabolic syndrome (MetS) in the Chinese population. To examine the effect of the dietary pattern reflecting CRP with MetS, a cross-sectional study was based on the health checkup data from the Beijing MJ Health Screening Centers between 2008 and 2018. The CRP-related dietary pattern was derived from 17 food groups using reduced-rank regression. Participants were divided into five groups according to the quintiles of dietary pattern score. Multivariate logistic regression was then applied to estimate the odds ratios (OR) and 95% confidence intervals (CIs) for the quintiles of diet pattern score related to MetS and its four components. Of the 90,130 participants included in this study, 11,209 had MetS. A CRP-related dietary pattern was derived, characterized by a higher consumption of staple food, fresh meat, processed products, and sugar-sweetened beverages but a lower intake of honey and jam, fruits, and dairy products. Compared with participants in the lowest quintile (Q1), participants in the higher quintiles were associated with increased risks of MetS in a dose-response manner after adjustment for potential confounders ( for linear trend < 0.001), the ORs for Q2 to Q5 were 1.10 (95% CI: 1.02-1.19), 1.14 (95% CI: 1.05-1.22), 1.23 (95% CI: 1.15-1.33), and 1.49 (95% CI: 1.38-1.61), respectively. Moreover, the effects were stronger among individuals aged 50 years or older. A CRP-related dietary pattern was associated with the risk of MetS. It provides new insights that dietary intervention to achieve a lower inflammatory level could potentially prevent MetS.
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http://dx.doi.org/10.3390/nu14132566DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9268474PMC
June 2022

Association of Sleep Duration with Hyperuricemia in Chinese Adults: A Prospective Longitudinal Study.

Int J Environ Res Public Health 2022 07 1;19(13). Epub 2022 Jul 1.

Department of Epidemiology & Biostatistics, School of Public Health, Peking University, Beijing 100191, China.

Little is known about the association of sleep duration with hyperuricemia. Especially lacking is evidence from longitudinal studies. Based on the MJ Health Examination Database in Beijing, China, a prospective study was designed. Participants were classed into short, normal, and long groups by sleep duration. The Cox regression model was used to estimate the hazard risk of hyperuricemia for short or long sleep duration compared with the normal group after adjusting for potential confounders. During a median 3.08 years follow-up, 4868 (14.31%) incident hyperuricemia events were documented among 34,025 participants with a crude incidence rate of 39.49 per 1000 persons. Years after adjusting for potential confounders, a 7% higher risk of hyperuricemia in the short sleep duration group (<7 h, 95% confidence interval: 1.01-1.14) and a 15% lower risk in the long sleep duration group (≥8 h, 95%CI: 0.74-0.97) were found compared with the normal group (7-8 h) ( for trend < 0.001). Nevertheless, the association of the short sleep duration group was marginally significant after further adjustment of the count of white blood cells (hazard ratio: 1.07, 95%CI: 1.00-1.13). Sleep duration was inversely associated with hyperuricemia, which highlights the public health significance of sufficient sleep duration for preventing hyperuricemia.
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http://dx.doi.org/10.3390/ijerph19138105DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9265381PMC
July 2022

Coupling riboflavin de novo biosynthesis and cytochrome expression for improving extracellular electron transfer efficiency in Shewanella oneidensis.

Biotechnol Bioeng 2022 Jul 7. Epub 2022 Jul 7.

Frontier Science Center for Synthetic Biology and Key Laboratory of Systems Bioengineering (Ministry of Education), Tianjin University, Tianjin, China.

Shewanella oneidensis MR-1, as a model exoelectrogen with divergent extracellular electron transfer (EET) pathways, has been widely used in microbial fuel cells (MFCs). The electron transfer rate is largely determined by riboflavin (RF) and c-type cytochromes (c-Cyts). However, relatively low RF production and inappropriate amount of c-Cyts substantially impede the capacity of improving the EET rate. In this study, coupling of riboflavin de novo biosynthesis and c-Cyts expression was implemented to enhance the efficiency of EET in S. oneidensis. First, the upstream pathway of RF de novo biosynthesis was divided into four modules, and the expression level of 22 genes in above four modules was fine-tuned by employing promoters with different strengths. Among them, genes zwf*, glyA, and ybjU which exhibited optimal RF production were combinatorially overexpressed, leading to the enhancement of maximum output power density by 166%. Second, the diverse c-Cyts genes were overexpressed to match high RF production, and omcA was selected for further combination. Third, RF de novo biosynthesis and c-Cyts expression were combined, resulting in 2.34-fold higher power output than the parent strain. This modular and combinatorial manipulation strategy provides a generalized reference to advance versatile practical applications of electroactive microorganisms.
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http://dx.doi.org/10.1002/bit.28172DOI Listing
July 2022

Association of abnormal vaginal microflora and HPV infection in cervical precancerous lesions: a retrospective study.

J Infect Dev Ctries 2022 06 30;16(6):1089-1095. Epub 2022 Jun 30.

Department of Obstetrics and Gynecology, China-Japan Friendship Hospital, Beijing, China.

Introduction: The aim of this study was to analyze the correlation between abnormal vaginal microflora and different types of human papillomavirus (HPV) infection and cervical precancerous lesions during the perimenopausal period.

Methodology: This retrospective study included women patients who underwent liquid-based cytologic test (LBC), HPV test, leucorrhea routine test, or routine urine test at the China-Japan Friendship Hospital between October 2019 and January 2020. A cut-off of 45 years was used as the cut-off age for menopause. The positivity and subtypes of HPV were determined using a chip-based assay. Vaginal microflora was determined using an HB-2012a flow-through hybridization instrument.

Results: A total of 132 patients were included in this study. 97 patients were younger than 45 years of age, with a median age of 35 (8.0), and 35 patients ≥ 45 years of age, with a median age of 55 (11.0). There were no significant differences in cytology, type of cervical lesion, HPV type, and common pathogens of the reproductive tract (all p > 0.05). The multivariable analysis showed that only HPV-16 infection lesions (OR: 2.825, 95% CI: 1.121-7.120, p = 0.028), Chlamydia trachomatis infection (OR: 0.142, 95% CI: 0.024-0.855, p =0.033), and Mycoplasma infection (OR: 7.750, 95% CI: 1.603-37.474, p = 0.011) were independent risk factors for cervical precancerous lesions. The menopausal status (with age < 45 or > 45 years as its surrogate) was not associated with cervical precancerous lesions.

Conclusions: Menopause was not associated with cervical precancerous lesions. The results suggest that the prevention and treatment of HPV-16, Chlamydia trachomatis infection, and Mycoplasma infection could be significant to prevent the occurrence of cervical precancerous lesions.
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http://dx.doi.org/10.3855/jidc.15961DOI Listing
June 2022

Quantitative assessment of gait characteristics in patients with Parkinson's disease using 2D video.

Parkinsonism Relat Disord 2022 Jun 30;101:49-56. Epub 2022 Jun 30.

Department of Neurology, Tianjin Huanhu Hospital, Tianjin Key Laboratory of Cerebral Vascular and Neurodegenerative Diseases, Tianjin Neurosurgical Institute, Tianjin, 300350, China. Electronic address:

Introduction: Gait disorders are common in patients with Parkinson's disease (PD), and objective, quantifiable, and portable evaluation methods are needed in clinical practice. We assessed the differences in gait characteristics between patients with PD and healthy controls (HCs) using two-dimensional (2D) video and explored the diagnostic value of 2D video for early-stage PD.

Methods: Sixty-eight patients with PD and 48 HCs were recruited. All participants walked a standard 5-m track while recorded using mobile phones. The differences in gait characteristics between the groups and gait parameters of patients with early-stage PD were analyzed. Receiver operating characteristic analysis was used to evaluate the discrimination activity of gait parameters.

Results: Compared with HCs, patients with PD had shorter step length (S/M, p < 0.001/0.001), faster cadence (p = 0.044), slower walking speed (p < 0.001), smaller arm-swing angle (S/M, p < 0.001/0.001), slower arm-swing velocity (S/M, p < 0.001/0.001), and greater variability and asymmetry. The patients with early-stage PD had similar characteristics. After combined step length and variability and asymmetry of arm-swing angle, diagnostic sensitivity and specificity reached 84.6% and 89.6%, respectively, and an area under the curve of 0.91 (0.84-0.97).

Conclusion: We proposed a new method to improve the sensitivity of early-stage PD diagnosis using 2D video, which, as a portable and objective method, could be used to evaluate gait disorders in PD, including in early-stage PD. Combined diagnostic analysis of step length and variability and asymmetry of arm-swing angle could improve the sensitivity of PD diagnosis and was helpful in distinguishing patients with early-stage PD from HCs.
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http://dx.doi.org/10.1016/j.parkreldis.2022.06.012DOI Listing
June 2022

Using a mediating model of death attitude and meaning in life to understand nursing students attitude about hospice care.

Nurse Educ Today 2022 Sep 24;116:105448. Epub 2022 Jun 24.

School of Nursing, Massachusetts College of Pharmacy and Health Sciences, Boston, MA 02115, USA.

Background: Nurse-led hospice teams are an emerging trend in contemporary health care practice in China. However, Chinese nursing students are often ill-prepared to work in hospice care. Study findings on the attitude of nursing students toward the care of people who are dying and death varied among different countries and regions.

Objectives: To propose and empirically test a mediating model that examines how death attitude and meaning in life interact to affect Chinese undergraduate nursing students and their attitude toward the care of people who are dying.

Design: A cross-sectional study.

Participants: A convenience sample of 1410 Chinese undergraduate nursing students.

Methods: Data were collected from July to December 2020 using Chinese versions of the Frommelt Attitude Toward Care of the Dying Scale, Form B (FATCOD-B-C), Death Attitude Profile-Revised (DAP-R-C), and Purpose in Life Test (CPLT-C). SPSS version 22.0 and AMOS version 24.0 were used for statistical analysis.

Results: The average FATCOD-B-C score was 98.28 ± 8.02. Death attitude and meaning in life were positively correlated with attitude toward care of the dying (p < 0.001), but death attitude was negatively correlated with meaning in life (p < 0.001). Pathway analysis showed that fear of death was significantly and positively correlated with attitude toward care of people who are dying. A mediating role was found among neutral acceptance, escape acceptance, and meaning in life to some extent. Meaning in life suppressed effects among the model.

Conclusion: This study revealed that Chinese undergraduate nursing students' attitude toward care of the dying was a positive nurturing process. Future nurse education should first identity the fear of death, neutral acceptance, or escape acceptance of nursing students' death attitude, and then develop a hospice curriculum based on fear management.
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http://dx.doi.org/10.1016/j.nedt.2022.105448DOI Listing
September 2022

The potential ecological risk assessment of soil heavy metals using self-organizing map.

Sci Total Environ 2022 Jun 27;843:156978. Epub 2022 Jun 27.

Faculty of Humanities and Social Sciences, University of Nottingham Ningbo, Ningbo 315100, China.

Heavy metal pollution control zoning aiming at the health maintenance of watershed soil ecosystem has become an important means of soil environmental protection and governance. Based on the self-organizing map (SOM), this study classifies the data sets of eight heavy metals such as Co, Cd, Zn, Cr, Cu, Pb, Ni, and Tl in 354 samples, calculates the potential ecological risk value of soil heavy metals in combination with the potential Hakansom Risk index (HRI), and uses the geographic information system (GIS) for visualization. In the research results, SOM has divided five soil ecological risk categories. The highest average ecological risk value of 85.95 is found in cluster IV, which is clustered and distributed in urban development areas in the upper reaches of the river. The average ecological risk values of cluster I and cluster V are relatively close at 79.64 and 79.19, respectively. Cluster I and cluster V are distributed in the north of the river in a linear and cluster manner, respectively, and are located on a concave bank with a relatively gentle slope. The average ecological risk of soil pollution in cluster II is 77.59, which is linearly distributed on both banks of the river. The ecological risk of soil pollution in cluster III is the lowest (74.39), mainly scattered in the south of rivers with less human activities. The study further identified the environmental factors that affect the soil ecological risk value in different cluster units and put forward the classified and differentiated management and control strategies for different cluster units. The research shows that SOM can cluster the data sets of heavy metals with high sensitivity and low threshold through competitive learning to effectively provide the distribution information of abnormal soil ecological risk areas. This information is helpful for urban environmental management departments and planning departments to take targeted management and recovery measures to avoid the health risks related to soil heavy metal pollution.
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http://dx.doi.org/10.1016/j.scitotenv.2022.156978DOI Listing
June 2022

High-Mobility Group Box 1 Inhibitor BoxA Alleviates Neuroinflammation-Induced Retinal Ganglion Cell Damage in Traumatic Optic Neuropathy.

Int J Mol Sci 2022 Jun 16;23(12). Epub 2022 Jun 16.

State Key Laboratory of Ophthalmology, Zhongshan Ophthalmic Center, Sun Yat-sen University, Guangzhou 510060, China.

Traumatic optic neuropathy (TON) is a significant cause of vision loss and irreversible blindness worldwide. It is defined as retinal ganglion cell death and axon degeneration caused by injury. Optic nerve crush (ONC), a well-validated model of TON, activates retinal microglia and initiates neuroinflammation. High-mobility group box 1 (HMGB1), a non-histone chromosomal binding protein in the nucleus of eukaryotic cells, is an important inducer of microglial activation and pro-inflammatory cytokine release. The purpose of this study was to examine the protective effects and mechanism of the HMGB1 inhibitor BoxA to neuroinflammation-induced retinal ganglion cells (RGCs) damage in traumatic optic neuropathy. For that purpose, an optic nerve crush model was established in C57BL/6J mice at 10-12 weeks. Model mice received an intravitreal injection of PBS and the HMGB1 inhibitor BoxA. Our data demonstrated that HMGB1 expression increased after optic nerve crush. Retinal ganglion cell function and morphology were damaged, and retinal ganglion cell numbers were reduced after optic nerve crush. Intravitreal injection of BoxA after ONC can alleviate damage. Furthermore, BoxA reduced microglial activation and expression levels of nuclear factor κB (NF-kB), nucleotide-binding domain, leucine-rich repeat containing protein 3 (NLRP3), and apoptosis-associated speck-like protein containing a CARD (ASC) in experimental ONC mice. In summary, HMGB1 mediates NLRP3 inflammasome via NF-kB to participate in retinal inflammatory injury after ONC. Thus, intravitreal injection of BoxA has potential therapeutic benefits for the effective treatment of RGC death to prevent TON.
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http://dx.doi.org/10.3390/ijms23126715DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9223527PMC
June 2022

Measurement properties of performance-based measures to assess physical function in knee osteoarthritis: A systematic review.

Clin Rehabil 2022 Jun 14:2692155221107731. Epub 2022 Jun 14.

School of Nursing, 12465Peking University, China, Beijing, China.

Objective: To systematically review the measurement properties of performance-based measures to assess physical function in people with knee osteoarthritis.

Data Sources: PubMed, Web of Science, Embase, Scopus, CINAHL, and PsycINFO were searched in May 2022.

Methods: This study was conducted in accordance with the guidelines recommended by the Preferred Reporting Items for Systematic Reviews and Meta-Analysis. Clinical trials on the psychometric properties of performance-based tools for measuring physical function in people with knee osteoarthritis were included. Two reviewers independently rated measurement properties using the COnsensus-based Standards for the selection of health Measurement INstruments (COSMIN). "Best evidence synthesis" was made using COnsensus-based Standards for the selection of health Measurement INstruments outcomes and the quality of findings.

Results: Thirty-six out of 3425 publications were eligible for inclusion. Thirty-two performance-based measures were evaluated including 26 single-activity measures and 6 multi-activity measures. Measurement properties evaluated included internal consistency (2 measures), reliability (23 measures), measurement error (20 measures), hypotheses testing for construct validity (22 measures), and responsiveness (23 measures). On balance of the limited evidence, the walk 40 m fast-paced test and 6-minute walking test were the best rated walking tests. The 30-second chair stand test and timed up and go test were the best rated sit-to-stand tests. The Performance Tests Measures and Physical Activity Restrictions may be the suitable multi-activity measures for knee osteoarthritis.

Conclusions: Further good quality research investigating the measurement properties, and in particular, the measurement error of performance-based measures in patients with knee osteoarthritis is needed.
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http://dx.doi.org/10.1177/02692155221107731DOI Listing
June 2022

Ginkgo Biloba Extract Ameliorates Scopolamine-induced Memory Deficits via Rescuing Synaptic Damage.

Curr Med Sci 2022 Jun 8;42(3):474-482. Epub 2022 Jun 8.

Department of Pediatrics, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, 430030, China.

Objective: Alzheimer's disease (AD) is the most common age-related neurodegenerative disorder. Emerging evidence suggests that synaptic dysfunction is associated with the onset and progression of AD. Interestingly, Ginkgo biloba extract (EGb) is one of the most frequently investigated herbal medicines for enhancing cognition and alleviating neurodegenerative dementia. This study aimed to investigate the effect and the mechanism of EGb on AD-like synaptic disorders.

Methods: Scopolamine (SCO)-induced rats were used to mimic AD-like memory deficits. Morris water maze test and fear conditioning test were conducted to evaluate the memory status of rats in response to different treatments. Then, the synapse alterations were assessed by Golgi staining, and Western blotting was conducted to assess the protein expression of PSD95, GluN2B, synapsin-1, and synaptophysin. Reverse transcription quantitative polymerase chain reaction was applied to detect the mRNA expression of PSD95 and the levels of miR-1-3p/miR-206-3p.

Results: EGb supplement alleviated the learning and memory deficits induced by SCO in behavioral experiments. Moreover, EGb treatment attenuated synaptic damage elicited by SCO, manifested as increased dendritic spine density and the proportion of mushroom-type spines in hippocampal neurons. Further investigation indicated that EGb rescued the expression of synaptic-related proteins, especially PSD95, and decreased the levels of miR-1-3p/miR-206-3p in the rat hippocampus.

Conclusion: The application of EGb effectively treats SCO-induced memory impairments probably by suppressing miR-1-3p/miR-206-3p and elevating the expression of PSD95.
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http://dx.doi.org/10.1007/s11596-022-2582-8DOI Listing
June 2022

Characteristics of Kawasaki Disease Before and After the COVID-19 Pandemic in a Large Pediatric Heart Disease Center.

Front Pediatr 2022 17;10:895408. Epub 2022 May 17.

Department of Pediatrics, Second Affiliated Hospital and Yuying Children's Hospital of Wenzhou Medical University, Wenzhou, China.

Background: Kawasaki disease (KD) is an acute febrile systemic vasculitis of unknown etiology. After the pandemic of coronavirus disease 2019 (COVID-19), some children infected by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) showed clinical symptoms similar to KD, indicating a close relationship between KD and SARS-CoV-2. Therefore, we designed this retrospective study to analyze the characteristics of KD patients before and after the COVID-19 pandemic.

Methods: We retrospectively collected demographic and laboratory data of KD patients in Yuying Children's Hospital of Wenzhou Medical University from 1 January 2015 to 31 December 2020. Yuying Children's Hospital of Wenzhou Medical University is located in eastern China and is the largest pediatric heart disease center in the region, which includes a population of nearly 10 million. We studied the characteristics of KD patients and analyzed the changes in these characteristics before and after the emergence of SARS-CoV-2 in this area.

Results: The analysis revealed the following novel features: (1) Under the influence of the COVID-19 pandemic, the onset age of Kawasaki disease became younger. (2) After the occurrence of COVID-19, the hospitalization days of KD patients were shorter than before the pandemic. (3) After the occurrence of COVID-19, the albumin of KD patients was higher than before the pandemic. (4) The COVID-19 pandemic did not have a significant effect on the incidence of coronary artery lesions (CALs) in Kawasaki disease.

Conclusion: After the COVID-19 outbreak, the characteristics of KD patients showed a younger trend of age, shorter hospitalization days and higher levels of albumin, but the incidence of CALs did not change significantly.
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http://dx.doi.org/10.3389/fped.2022.895408DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9152138PMC
May 2022

Research on the effect and underlying molecular mechanism of Cangzhu in the treatment of gouty arthritis.

Eur J Pharmacol 2022 Jul 26;927:175044. Epub 2022 May 26.

School of Pharmacy, Jiangxi University of Traditional Chinese Medicine, Nanchang, China. Electronic address:

Objective: We aimed to identify the active ingredients and elucidate the underlying mechanism of action of Atractylodes lancea (Thunb.) DC (namely, Cangzhu) for the treatment of gouty arthritis (GA) based on network pharmacology methods. These findings are expected to provide a theoretical basis for the clinical treatment of GA.

Methods: We used monosodium urate (MSU)-induced GA rats as a model to test the overall efficacy of Cangzhu in vivo. Then, the components of the Cangzhu decoction were analyzed and identified, and we screened the active ingredients and their targets. The GA disease targets were predicted by GeneCards and Disgenet databases and found to overlap in both databases. The STRING database was used to construct a protein-protein interaction network, followed by identification of the hub genes using Network Analyzer. Thereafter, Cytoscape software (version 3.8.2) was applied to construct a network for drug-active ingredient-key targets. Next, we applied cluego, a plug-in of Cytoscape, to perform gene ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) signal pathway enrichment analyses. Additionally, molecular docking was used to verify the characteristics of the key candidate components interacting with the hub therapeutic targets. Finally, we established an inflammatory injury model of LPS using RAW264.7 macrophages and used it to experimentally validate the critical active ingredients.

Results: Cangzhu effectively protected against gouty arthritis in vivo, and network pharmacology results revealed various active ingredients in Cangzhu, such as wogonin, atractylenolide I and atractylenolide II. These compounds were found to act on 16 hub targets, including tumor necrosis factor-α (TNF-α), interleukin 6 (IL-6), interleukin-1β (IL-1β), prostaglandin-endoperoxide synthase 2 (PTGS2), recombinant mitogen-activated protein kinase 14 (MAPK14) and transcription factor p65 (RELA), which have significant effects on regulating inflammatory factors and apoptosis-related pathways to improve the proinflammatory or anti-inflammatory imbalance in the body, and this may be one of the underlying mechanisms of Cangzhu in anti-GA.

Conclusion: Our findings revealed that Cangzhu comprises multiple active components that exert various targeted effects during GA treatment. These findings provide relevant insights to illuminate the mechanism of Cangzhu in the treatment of GA and provide a reference for further experimental research.
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http://dx.doi.org/10.1016/j.ejphar.2022.175044DOI Listing
July 2022

A live attenuated virus-based intranasal COVID-19 vaccine provides rapid, prolonged, and broad protection against SARS-CoV-2.

Sci Bull (Beijing) 2022 Jul 26;67(13):1372-1387. Epub 2022 May 26.

State Key Laboratory of Molecular Vaccinology and Molecular Diagnostics, National Institute of Diagnostics and Vaccine Development in Infectious Diseases, School of Life Sciences, School of Public Health, Xiamen University, Xiamen 361102, China.

Remarkable progress has been made in developing intramuscular vaccines against severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2); however, they are limited with respect to eliciting local immunity in the respiratory tract, which is the primary infection site for SARS-CoV-2. To overcome the limitations of intramuscular vaccines, we constructed a nasal vaccine candidate based on an influenza vector by inserting a gene encoding the receptor-binding domain (RBD) of the spike protein of SARS-CoV-2, named CA4-dNS1-nCoV-RBD (dNS1-RBD). A preclinical study showed that in hamsters challenged 1 d after single-dose vaccination or 9 months after booster vaccination, dNS1-RBD largely mitigated lung pathology, with no loss of body weight. Moreover, such cellular immunity is relatively unimpaired for the most concerning SARS-CoV-2 variants, especially for the latest Omicron variant. In addition, this vaccine also provides cross-protection against H1N1 and H5N1 influenza viruses. The protective immune mechanism of dNS1-RBD could be attributed to the innate immune response in the nasal epithelium, local RBD-specific T cell response in the lung, and RBD-specific IgA and IgG response. Thus, this study demonstrates that the intranasally delivered dNS1-RBD vaccine candidate may offer an important addition to the fight against the ongoing coronavirus disease 2019 pandemic and influenza infection, compensating limitations of current intramuscular vaccines.
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http://dx.doi.org/10.1016/j.scib.2022.05.018DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9134758PMC
July 2022

Comprehensive Metabolomic Comparison of Five Cereal Vinegars Using Non-Targeted and Chemical Isotope Labeling LC-MS Analysis.

Metabolites 2022 May 10;12(5). Epub 2022 May 10.

Bioinformation and DDBJ Center, National Institute of Genetics, Mishima 411-8540, Japan.

Vinegar is used as an acidic condiment and preservative worldwide. In Asia, various black vinegars are made from different combinations of grains, such as Sichuan bran vinegar (SBV), Shanxi aged vinegar (SAV), Zhenjiang aromatic vinegar (ZAV), and Fujian Monascus vinegar (FMV) in China and Ehime black vinegar in Japan (JBV). Understanding the chemical compositions of different vinegars can provide information about nutritional values and the quality of the taste. This study investigated the vinegar metabolome using a combination of GC-MS, conventional LC-MS, and chemical isotope labeling LC-MS. Different types of vinegar contained different metabolites and concentrations. Amino acids and organic acids were found to be the main components. Tetrahydroharman-3-carboxylic acid and harmalan were identified first in vinegar. Various diketopiperazines and linear dipeptides contributing to different taste effects were also detected first in vinegar. Dipeptides, 3-phenyllactic acid, and tyrosine were found to be potential metabolic markers for differentiating vinegars. The differently expressed pathway between Chinese and Japanese vinegar was tryptophan metabolism, while the main difference within Chinese vinegars was aminoacyl-tRNA biosynthesis metabolism. These results not only give insights into the metabolites in famous types of cereal vinegar but also provide valuable knowledge for making vinegar with desirable health characteristics.
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http://dx.doi.org/10.3390/metabo12050427DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9144210PMC
May 2022

Disturbance observer-based adaptive position control for a cutterhead anti-torque system.

PLoS One 2022 24;17(5):e0268897. Epub 2022 May 24.

State Key Laboratory of Fluid Power and Mechatronic Systems, Zhejiang University, Hangzhou, China.

To conveniently replace worn cutterhead tools in complicated strata, a novel cutterhead attitude control mechanism was recently designed. Meanwhile, the mechanism also causes an engineering problem of how to control a matching cutterhead anti-torque system (CATS) effectively, which is used to prevent a drive box of the cutterhead from rotation during a complex excavation process. In this paper, a disturbance observer-based adaptive position controller is proposed for the CATS. The proposed method presents a nonlinear adaptive controller with adaptation laws to compensate for the unknown time-varying load torque and damping uncertainty in the system. Based on the disturbance observer method and sliding mode control, an asymptotically stable controller proven by Lyapunov theory is constructed using the back-stepping technique. In addition, a virtual test rig based on MATLAB and AMESim co-simulation is built to verify the validity of the proposed controller. The simulation results show that the proposed method has good performance for tracking tasks in the presence of uncertainties compared with PID control. Together, the data support targeting disturbance observer-based adaptive position control as a potential control strategy for cutterhead anti-torque systems.
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http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0268897PLOS
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9129045PMC
May 2022

Hydrogen sulfide supplement preserves mitochondrial function of retinal ganglion cell in a rat glaucoma model.

Cell Tissue Res 2022 Aug 20;389(2):171-185. Epub 2022 May 20.

Department of Ophthalmology, Ruijin Hospital Affiliated Medical School, Shanghai Jiaotong University, Shanghai, China.

Glaucoma is a neurodegenerative disease of visual system characterized by gradual loss of retinal ganglion cells (RGC). Since mitochondrial dysfunction of RGC is significantly involved in the pathological mechanisms of glaucoma, and hydrogen sulfide (HS) takes part in the pathogeny of glaucoma and shows promising potential in restoring mitochondrial function in other neurons, the authors aimed to investigate the impact of HS on mitochondrial function of RGC with a rat glaucoma model. An established chronic ocular hypertension (COH) rat model induced by injection of cross-linking hydrogel into anterior chamber was adopted, and a HS donor, sodium hydrosulfide (NaHS), was selected to treat rats through intraperitoneal injection. After a period of 4 weeks, RGCs were isolated from the subjected rats with an immunopanning method and went through evaluations of mitochondrial membrane potential (MMP), mitochondrial permeability transition pore (MPTP) opening, intracellular Ca2 + level, reactive oxygen species (ROS) level, and cytosolic Cytochrome C distribution. The results showed that the mitochondrial function of RGC in experimental glaucoma was markedly improved by HS supplement, being presented as stabilization of MMP, alleviation of MPTP opening, improvement of intracellular Ca hemostasis, reduction of ROS accumulation, and inhibition of Cytochrome C release. Our study implicated that preservation of mitochondrial function by HS probably plays a key role in protecting RGC in the context of glaucomatous neuropathy, and it is worth further deepgoing research to benefit the development of glaucoma treatment.
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http://dx.doi.org/10.1007/s00441-022-03640-xDOI Listing
August 2022

Molecular signaling in temporomandibular joint osteoarthritis.

J Orthop Translat 2022 Jan 10;32:21-27. Epub 2021 Sep 10.

Faculty of Pharmaceutical Sciences, Shenzhen Institute of Advanced Technology, Chinese Academy of Sciences, Shenzhen, 518055, China.

Objective: Temporomandibular joint (TMJ) osteoarthritis (OA) is a type of TMJ disorders with clinical symptoms of pain, movement limitation, cartilage degeneration and joint dysfunction. This review article is aiming to summarize recent findings on signaling pathways involved in TMJ OA development and progression.

Methods: Most recent findings in TMJ OA studies have been reviewed and cited.

Results: TMJ OA is caused by inflammation, abnormal mechanical loading and genetic abnormalities. The molecular mechanisms related to TMJ OA have been determined using different genetic mouse models. Recent studies demonstrated that several signaling pathways are involved in TMJ OA pathology, including Wnt/β-catenin, TGF-β and BMP, Indian Hedgehog, FGF, NF-κB, and Notch pathways, which are summarized in this review article. Alterations of these signaling pathways lead to the pathological changes in TMJ tissues, affecting cartilage matrix degradation, catabolic metabolism and chondrocyte apoptosis.

Conclusion: Multiple signaling pathways were involved in the pathological process of TMJ OA. New therapeutic strategies, such as stem cell application, gene editing and other techniques may be utilized for TMJ OA treatment.

The Translational Potential Of This Article: TMJ OA is a most important subtype of TMJ disorders and may lead to substantial joint pain, dysfunction, dental malocclusion, and reduced health-related quality of life. This review article summarized current findings of signaling pathways involved in TMJ OA, including Wnt/β-catenin, TGF-β and BMP, Indian Hedgehog, FGF, NF-κB, and Notch pathways, to better understand the pathological mechanisms of TMJ OA and define the molecular targets for TMJ OA treatment.
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http://dx.doi.org/10.1016/j.jot.2021.07.001DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9072795PMC
January 2022

The effect of anti-alcoholic gastric ulcer before and after vinegar-processed Yuanhu Zhitong prescription based on spectral-effect relationship.

Biomed Chromatogr 2022 Aug 15;36(8):e5410. Epub 2022 Jun 15.

Key Laboratory of Basic and Application Research of Beiyao, Heilongjiang University of Chinese Medicine, Ministry of Education, Harbin, China.

The aim of this study was to screen out the active ingredients of Yuanhu Zhitong prescription (YZP) before and after vinegar processing to play an anti-alcoholic gastric ulcer role through spectrum-effect relationship. First, the fingerprint of 16 batches of YZP was studied using the ultra-high-performance liquid chromatography-quadrupole mass spectrometry detector analysis (UPLC-QDA) method. Second, gastric lesion was induced by anhydrous ethanol. The degree of gastric mucosa injury was evaluated by hematoxylin and eosin staining, and the contents of malondialdehyde and tumor necrosis factor α and superoxide dismutase were detected using the enzyme-linked immunosorbent assay kit. Sixteen batches of YZP were analyzed using the spectrum-effect relationship method. Finally, absorption, distribution, metabolism, and excretion (ADME) was used to evaluate the bioavailability of potential compounds. The results showed that the UPLC-QDA method could successfully establish the fingerprint of YZP. Hematoxylin and eosin staining and biochemical indicators showed that YZP had obvious anti-alcoholic gastric ulcer action. Coptisine chloride, corydaline, berberine chloride, palmatine, imperatorin, and phellopterin were screened out using the spectrum-effect method, and all of them possessed good bioavailability. The results of this study suggest that YZP plays an anti-ulcer role by exerting antioxidant and anti-inflammatory effects through six main active components.
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http://dx.doi.org/10.1002/bmc.5410DOI Listing
August 2022

Efficacy and safety of autologous hematopoietic stem cell transplantation in the treatment of malignant lymphoma after chemotherapy: a systematic review and meta-analysis.

Transl Cancer Res 2022 Apr;11(4):888-896

Neoplastic Hematologic Disorder, Affiliated Hospital of Xiangnan University, Chenzhou, China.

Background: Autologous hematopoietic stem cell transplantation (AHSCT) is a common method for the clinical treatment of malignant lymphomas that recur after conventional chemotherapy. It has been reported that its efficacy is better than conventional chemotherapy, but the efficacy of its first-line treatment is controversial, and the existing clinical randomized controlled trials have not yet reached a unified conclusion. This work intended to use meta-analysis to systematically evaluate the efficacy and safety of AHSCT in the treatment of malignant lymphoma after high-dose chemotherapy, and draw reliable conclusions to provide reference and basis for clinical application.

Methods: The inclusion and exclusion criteria were formulated based on the PICOIS principle. Relevant articles were retrieved from Medline, Excerpta Medica Database (EMBASE), Elton B. Stephens. Company (EBSCO), Ovid Technologies (OVID), China Biomedical Database, and Wanfang. The search period was limited the study published between January 1, 1980 and November 2021. The search terms included malignant lymphoma, autologous hematopoietic stem cell transplantation, AHSCT, high-dose chemotherapy, etc. The study subjects were diagnosed as malignant lymphoma patients. The experimental group was defined as AHSCT after high-dose chemotherapy, and the control group was defined as conventional chemotherapy (the chemotherapy regimen was not limited). The outcome indicators were overall survival (OS), complete remission rate [complete response (CR) + partial response (PR)], and event-free survival (EFS). RevMan5.3 software provided by the Cochrane Collaboration was used for meta-analysis.

Results: A total of 6 pieces of literature were included, with 264 cases in the experimental group and 389 cases in the control group. There was no risk of bias in the included literature. The intervention method in the control group was conventional chemotherapy (chemotherapy regimen was not limited). The differences in the rates of overall survival and progression-free survival between the groups were compared, and it was found that the overall survival between groups was [odds ratio (OR) =2.88; 95% confidence interval (CI): 1.78-4.66; Z=4.31; P<0.0001] and progression-free survival rate was (OR =2.70; 95% CI: 1.86-3.92, Z=5.21; P<0.00001).

Discussion: AHSCT treatment can significantly prolong the overall survival and progression-free survival rates of patients with malignant lymphoma after chemotherapy.
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http://dx.doi.org/10.21037/tcr-22-595DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9091041PMC
April 2022

The Significance of Symptoms in Predicting Coronary Artery Aneurysms of Kawasaki Disease, Especially in Female Patients.

Front Cardiovasc Med 2022 28;9:823862. Epub 2022 Apr 28.

Department of Pediatrics, Second Affiliated Hospital and Yuying Children's Hospital of Wenzhou Medical University, Wenzhou, China.

Background: Kawasaki disease (KD) is an acute febrile systemic vasculitis of unknown etiology that occurs during early childhood, commonly involving the coronary artery, and can lead to coronary artery aneurysms (CAAs).

Methods: The demographic, clinical, and laboratory data of KD patients without coronary artery lesions (N-CAL) and with CAA were collected during 2005-2020 at the Second Affiliated Hospital of Wenzhou Medical University. The patients were divided into the development cohort and the validation cohort. First, we compared the general information, symptoms, and laboratory data of N-CAL and CAA patients in the development cohort and the total cohort and screened out the different indices by logistic regression analysis. Then, we established three models and compared the area under the curve (AUC) values of the receiver operating characteristic (ROC) curves to identify meaningful models for CAA, which were further verified by decision curve analysis (DCA). Second, taking into account previous reports on the importance of gender to CAA, gender stratification was conducted.

Results: The analysis of clinical and blood indices revealed the following novel features: (i) Many factors were found to be related to CAA, including IVIG resistance and the symptoms of rash, oral changes, and cervical lymphadenopathy. (ii) The development cohort was analyzed by logistic regression, and three models were established. The ROC curves showed that Model 2, composed of IVIG resistance, rash, oral changes, and cervical lymphadenopathy, had a better AUC value and easily to evaluate in the prediction of CAA. (iii) The selected model for predicting CAA in the development cohort was further confirmed in the validation cohort through DCAs. (iv)We further compared the items enrolled in the three models above between the N-CAL and CAA groups by sex, and the results indicated that female KD patients without rash, oral changes, and cervical lymphadenopathy were more likely to develop CAA.

Conclusion: The absence of rash, oral changes, and cervical lymphadenopathy are risk factors for CAA, especially in female KD patients. Accurately recognizing symptoms, early diagnosis, and standard treatment for KD are key to reducing the incidence of CAA.
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http://dx.doi.org/10.3389/fcvm.2022.823862DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9095924PMC
April 2022

Phytoplankton package effect in oceanic waters: Influence of chlorophyll-a and cell size.

Sci Total Environ 2022 Sep 17;838(Pt 1):155876. Epub 2022 May 17.

School of Marine Sciences, Nanjing University of Information Science and Technology, Nanjing, China; The Key Laboratory of Space Ocean Remote Sensing and Application, Ministry of Natural Resources, China.

In this study, the interaction between the packaging effect (Q) and total chlorophyll-a concentration (C) or total size index (SI) was investigated to explore the potential bio-optical mechanism in phytoplankton cells in the global oceans. In addition, the long-term spatiotemporal characteristics of these interactions were necessary for grasping their variation. Numerous in situ surface measurements (phytoplankton pigment and absorption coefficients) from the global oceans were analyzed first, and then correlation and causality analyses were performed on the satellite-deduced Q, C, and SI in the global oceans during 2002-2020. The results show a negative correlation between Q and C or SI in the low latitudes (30°S-30°N) and a positive correlation in the middle latitudes (30°S-55°S and 30°N-55°N). The causality analysis reveals a mutual and asymmetric cause-effect relationship between Q and C or SI in the low latitudes. The stabilization effect of Q contributes to a 10%-50% variation in C and SI, with 40%-60% uncertainty of Q caused by C and SI in the low latitudes, which is inverse in the middle latitudes. The remaining contribution to each variable mainly originates from long-term trends and noise. Combining the analysis between Q and the irradiance, the balancing processes in phytoplankton cells are different in the low (phytoplankton-driving mode) and middle latitudes (irradiance-driving mode), which is related to photoacclimation and photoinhibition. The analyses provide insights into the quantitative interpretation of the relationship between Q and C or SI, which contribute knowledge that has not been previously reported.
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http://dx.doi.org/10.1016/j.scitotenv.2022.155876DOI Listing
September 2022

Osteoarthritis Pain.

Int J Mol Sci 2022 Apr 22;23(9). Epub 2022 Apr 22.

Faculty of Pharmaceutical Science, Shenzhen Institute of Advanced Technology, Chinese Academy of Sciences, Shenzhen 518055, China.

Joint pain is the hallmark symptom of osteoarthritis (OA) and the main reason for patients to seek medical assistance. OA pain greatly contributes to functional limitations of joints and reduced quality of life. Although several pain-relieving medications are available for OA treatment, the current intervention strategy for OA pain cannot provide satisfactory pain relief, and the chronic use of the drugs for pain management is often associated with significant side effects and toxicities. These observations suggest that the mechanisms of OA-related pain remain undefined. The current review mainly focuses on the characteristics and mechanisms of OA pain. We evaluate pathways associated with OA pain, such as nerve growth factor (NGF)/tropomyosin receptor kinase A (TrkA), calcitonin gene-related peptide (CGRP), C-C motif chemokine ligands 2 (CCL2)/chemokine receptor 2 (CCR2) and tumor necrosis factor alpha (TNF-α), interleukin-1beta (IL-1β), the NOD-like receptor (NLR) family, pyrin domain-containing protein 3 (NLRP3) inflammasome, and the Wnt/β-catenin signaling pathway. In addition, animal models currently used for OA pain studies and emerging preclinical studies are discussed. Understanding the multifactorial components contributing to OA pain could provide novel insights into the development of more specific and effective drugs for OA pain management.
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http://dx.doi.org/10.3390/ijms23094642DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9105801PMC
April 2022

The "Hand as Foot" teaching method in the grouping of axillary lymph nodes.

Asian J Surg 2022 May 9. Epub 2022 May 9.

Inner Mongolia Medical University, Department of Thyroid and Breast Surgery, The Affiliated Hospital of Inner Mongolia Medical University, Hohhot, Inner Mongolia, 010050, China. Electronic address:

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http://dx.doi.org/10.1016/j.asjsur.2022.04.105DOI Listing
May 2022

Erratum: MicroRNA-23a-3p Inhibits Mucosal Melanoma Growth and Progression through Targeting Adenylate Cyclase 1 and Attenuating cAMP and MAPK Pathways: Erratum.

Theranostics 2022 26;12(7):3578-3579. Epub 2022 Apr 26.

Key Laboratory of Carcinogenesis and Translational Research (Ministry of Education/Beijing), Department of Renal Cancer and Melanoma, Peking University Cancer Hospital and Institute, Beijing 100142, China.

[This corrects the article DOI: 10.7150/thno.30516.].
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http://dx.doi.org/10.7150/thno.72945DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9065187PMC
April 2022
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