Publications by authors named "Huan Sun"

133 Publications

Fabrication of customized Ti6AI4V heterogeneous scaffolds with selective laser melting: Optimization of the architecture for orthopedic implant applications.

Acta Biomater 2021 May 22;126:485-495. Epub 2021 Mar 22.

National Engineering Research Center for Biomaterials, Sichuan University, 610064, Chengdu, China; College of Biomedical Engineering, Sichuan University, 610064, Chengdu, China.

Orthopedic implants with heterogeneous porous structures were known as ideal bone osteointegration. This research introduced the selective laser melting (SLM), finite element analysis (FEA), and a hydrothermal process (HT) for manufacturing a three-level heterogeneous porous structure. The macroporous structure was designed via CAD and micropores were tuned via laser power regulation. A nano-size layer of hydroxyapatite crystals was coated by an HT process. The mechanical properties were reinforced via a core-shell structure with core reinforcement. The existence of micropores and nano-hydroxyapatite coating enhanced the in vitro proliferation of preosteoblasts and osteogenic cellular behaviors of rBMSCs. Thus, the three-level heterogeneous porous titanium implants could inspire researchers with potential clue of cyto-implant interaction mechanism, therefore building ideal orthopedic implants with accelerated osteointegration. STATEMENT OF SIGNIFICANCE: Porous structures of titanium implants play an important role in bone tissue regeneration; The geometrical environment influence cell behaviour and bone tissue ingrowth in all macro-/micro-/nanoscale. In this study, a novel method to fabricate heterogeneous scaffolds and its macro-/micro-/nanoscopic structures were studied. A CAD model was used to obtain the macroscopic structure and the insufficient laser power was introduced for porous microstructure. Therefore, a layer of nano hydroxyapatite was coated via hydrothermal process. Cytoproliferation and cytodifferentiation results indicated that a integrity of regular/irregular, macro-/micro-/nanoscale porous structure had advance in recruiting stem cells and promoting differentiation. This research is beneficial to the development of bone implants with better bone regeneration ability.
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http://dx.doi.org/10.1016/j.actbio.2021.03.040DOI Listing
May 2021

Tough Interfacial Adhesion of Bilayer Hydrogels with Integrated Shape Memory and Elastic Properties for Controlled Shape Deformation.

ACS Appl Mater Interfaces 2021 Mar 22;13(8):10457-10466. Epub 2021 Feb 22.

Wenzhou Institute, University of Chinese Academy of Sciences, Wenzhou 352001, China.

The weak adhesion between two hydrogel layers may lead to the delamination of bilayer hydrogels or low force transfer efficiency during deformation. Here, tough interfacial adhesive bilayer hydrogels with rapid shape deformation and recovery were prepared by simple attachment-heating of two gel layers. The bilayer hydrogels, composed of a shape memory gel (S-gel) and an elastic gel (E-gel), exhibited extremely tough interfacial adhesion between two layers (Γ ∼ 2200 J/m). The shape deformation and shape recovery of the bilayer hydrogels, tuned by "heating-stretching" mode and "stretching-heating-stretching" mode, were rapid (<5 s) and no delamination between two gel layers was detected during shape deformation. Based on the fast shape deformation and recovery, the bilayer hydrogels could mimic the flower and hand, and a gel gripper could be fabricated to catch the object in the hot water. This work provides a simple method to prepare tough adhesive bilayer hydrogels with controlled shape deformation.
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http://dx.doi.org/10.1021/acsami.0c22484DOI Listing
March 2021

Anatomical correlation between left atrium pulmonary vein ablation targets of atrial fibrillation and adjacent bronchi and pulmonary arteries by MSCT.

BMC Cardiovasc Disord 2021 Feb 10;21(1):84. Epub 2021 Feb 10.

Radiology Department, China-Japan Union Hospital of Jilin University, 126 Xiantai Street, Changchun, 130031, Jilin Province, China.

Background: The ablation targets of atrial fibrillation (AF) are adjacent to bronchi and pulmonary arteries (PAs). We used computed tomography (CT) to evaluate the anatomical correlation between left atrium (LA)-pulmonary vein (PV) and adjacent structures.

Methods: Data were collected from 126 consecutive patients using coronary artery CT angiography. The LA roof was divided into three layers and nine points. The minimal spatial distances from the nine points and four PV orifices to the adjacent bronchi and PAs were measured. The distances from the PV orifices to the nearest contact points of the PVs, bronchi, and PAs were measured.

Results: The anterior points of the LA roof were farther to the bronchi than the middle or posterior points. The distances from the nine points to the PAs were shorter than those to the bronchi (5.19 ± 3.33 mm vs 8.62 ± 3.07 mm; P < .001). The bilateral superior PV orifices, especially the right superior PV orifices were closer to the PAs than the inferior PV orifices (left superior PV: 7.59 ± 4.14 mm; right superior PV: 4.43 ± 2.51 mm; left inferior PV: 24.74 ± 5.26 mm; right inferior PV: 22.33 ± 4.75 mm) (P < .001).

Conclusions: The right superior PV orifices were closer to the bronchi and PAs than other PV orifices. The ablation at the mid-posterior LA roof had a higher possibility to damage bronchi. CT is a feasible method to assess the anatomical adjacency in vivo, which might provide guidance for AF ablation.
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http://dx.doi.org/10.1186/s12872-021-01881-2DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7877049PMC
February 2021

Hippocampal Aromatase Knockdown Aggravates Ovariectomy-Induced Spatial Memory Impairment, Aβ Accumulation and Neural Plasticity Deficiency in Adult Female Mice.

Neurochem Res 2021 May 8;46(5):1188-1202. Epub 2021 Feb 8.

Department of Neurobiology, Army Medical University, Chongqing, China.

Ovarian estrogens (mainly 17β estradiol, E2) have been involved in the regulation of the structure of hippocampus, the center of spatial memory. In recent years, high levels of aromatase (AROM), the estrogen synthase, has been localized in hippocampus; and this hippocampus-derived E2 seems to be functional in synaptic plasticity and spatial memory as ovarian E2 does. However, the contribution of ovarian E2 and hippocampal E2 to spatial memory and neural plasticity remains unclear. In this study, AROM-specific RNA interference AAVs (shAROM) were constructed and injected into the hippocampus of control or ovariectomized (OVX) mice. Four weeks later the spatial learning and memory behavior was examined with Morris water maze, the expression of hippocampal Aβ related proteins, selected synaptic proteins and CA1 synapse density, actin polymerization related proteins and CA1 spine density were also examined. The results showed that while OVX and hippocampal shAROM contributed similarly to most of the parameters examined, shAROM induced more increase in BACE1 (amyloidogenic β-secretase), more decrease in neprilysin (Aβ remover) and Profilin-1 (actin polymerization inducer). More importantly, combined OVX and shAROM treatment displayed most significant impairment of spatial learning and memory as well as decrease in synaptic plasticity compared to OVX or shAROM alone. In conclusion, the above results clearly demonstrated the crucial role of hippocampal E2 in the regulation of the structure and function of hippocampus besides ovarian E2, indicating that hippocampal E2 content should also be taken into consideration during estrogenic replacement.
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http://dx.doi.org/10.1007/s11064-021-03258-5DOI Listing
May 2021

Mushroom body output differentiates memory processes and distinct memory-guided behaviors.

Curr Biol 2021 Mar 20;31(6):1294-1302.e4. Epub 2021 Jan 20.

Graduate School of Life Sciences, Tohoku University, Sendai 980-8577, Japan. Electronic address:

The mushroom body (MB) of Drosophila melanogaster has multiple functions in controlling memory and behavior. However, circuit mechanisms that generate this functional diversity are largely unclear. Here, we systematically probed the behavioral contribution of each type of MB output neuron (MBON) by blocking during acquisition, retention, or retrieval of reward or punishment memories. We evaluated the contribution using two conditioned responses: memory-guided odor choice and odor source attraction. Quantitative analysis revealed that these conditioned odor responses are controlled by different sets of MBONs. We found that the valence of memory, rather than the transition of memory steps, has a larger impact on the patterns of required MBONs. Moreover, we found that the glutamatergic MBONs forming recurrent circuits commonly contribute to appetitive memory acquisition, suggesting a pivotal role of this circuit motif for reward processing. Our results provide principles how the MB output circuit processes associative memories of different valence and controls distinct memory-guided behaviors.
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http://dx.doi.org/10.1016/j.cub.2020.12.032DOI Listing
March 2021

Lasting effects of residential mobility during childhood on psychopathology among Chinese University students.

BMC Psychiatry 2021 01 15;21(1):45. Epub 2021 Jan 15.

Mental Health Center and Psychiatric Laboratory, the State Key Laboratory of Biotherapy, West China Hospital of Sichuan University, No. 28 Dianxin South street, Chengdu, 610041, Sichuan, China.

Background: Residential mobility during childhood increases risk of psychopathology in adulthood and is a common experience among Chinese children. This study investigated associations between number and age of first move, etiological risk factors for psychopathology, and common mental disorders in adolescence and early adulthood.

Methods: The sample included 39,531 undergraduates (84.5% completion rate) age 15-34 years in their first year at a Chinese comprehensive university in annual cross-sectional surveys during 2014-2018. Common mental disorders measured using standardised self-report instruments. Data analysed using logistic regression models and interaction analysis.

Results: Half of all students experienced one or more moves of residence before age 15 years. Outcomes of Depression, Somatisation, Obsessive-compulsive disorder, Hallucinations and Delusions, and Suicide attempts showed dose-response relationships with increasing number of moves. Other etiological risk factors, including childhood disadvantage and maltreatment, showed similar dose response relationships but did not confound associations with mobility. We found interactions between reporting any move and being a left-behind child on depression and somatisation; number of moves and younger age at first move on depression, somatisation, suicide attempts and hallucinations and delusions.

Conclusions: Residential mobility in childhood is associated with psychopathology in adulthood and this association increases with increasing number of moves. Mobility is also associated with childhood disadvantage and maltreatment but associations with psychopathology are independent of these factors. Multiplicative effects were shown for multiple moves starting at a younger age and if the participant had been a left-behind child.
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http://dx.doi.org/10.1186/s12888-020-03018-9DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7811262PMC
January 2021

Cross-species recognition of SARS-CoV-2 to bat ACE2.

Proc Natl Acad Sci U S A 2021 01;118(1)

Chinese Academy of Sciences Key Laboratory of Pathogenic Microbiology and Immunology, Institute of Microbiology, Chinese Academy of Sciences, 100101 Beijing, China;

The coronavirus disease 2019 (COVID-19) pandemic caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has emerged as a major threat to global health. Although varied SARS-CoV-2-related coronaviruses have been isolated from bats and SARS-CoV-2 may infect bat, the structural basis for SARS-CoV-2 to utilize the human receptor counterpart bat angiotensin-converting enzyme 2 (bACE2) for virus infection remains less understood. Here, we report that the SARS-CoV-2 spike protein receptor binding domain (RBD) could bind to bACE2 from (bACE2-Rm) with substantially lower affinity compared with that to the human ACE2 (hACE2), and its infectivity to host cells expressing bACE2-Rm was confirmed with pseudotyped SARS-CoV-2 virus and SARS-CoV-2 wild virus. The structure of the SARS-CoV-2 RBD with the bACE2-Rm complex was determined, revealing a binding mode similar to that of hACE2. The analysis of binding details between SARS-CoV-2 RBD and bACE2-Rm revealed that the interacting network involving Y41 and E42 of bACE2-Rm showed substantial differences with that to hACE2. Bats have extensive species diversity and the residues for RBD binding in bACE2 receptor varied substantially among different bat species. Notably, the Y41H mutant, which exists in many bats, attenuates the binding capacity of bACE2-Rm, indicating the central roles of Y41 in the interaction network. These findings would benefit our understanding of the potential infection of SARS-CoV-2 in varied species of bats.
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http://dx.doi.org/10.1073/pnas.2020216118DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7817217PMC
January 2021

Mortalité, congé et arythmie chez les patients ayant la COVID-19 et une atteinte cardiaque.

CMAJ 2020 Nov;192(48):E1648-E1656

Service de cardiologie (Si, Du, Sun, Liu, Yang), troisième hôpital de l'Université du Jilin, Centre de recherche en biologie moléculaire de la province du Jilin pour la médecine de précision des maladies cardiovasculaires majeures, Changchun, Chine; Service d'échographie (Ni), troisième hôpital de l'Université du Jilin, Changchun, Chine; Institut de transplantation d'organes (Yang), hôpital Tongji, Collège médical Tongji, Université Huazhong des sciences et technologies, Wuhan, Chine; Unité des soins intensifs (Jiang), troisième hôpital de l'Université du Jilin, Changchun, Chine; le laboratoire Hull Family (Massé, J. Nanthakumar, Bhaskaran, K. Nanthakumar), Centre de cardiologie Peter Munk, Hôpital général de Toronto, Université de Toronto, Toronto, Ont.; Département d'épidémiologie et de biostatistique (Jin), École de santé publique, Université du Jilin, Changchun, Jilin, Chine.

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http://dx.doi.org/10.1503/cmaj.200879-fDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7721390PMC
November 2020

Cardiac injury is associated with inflammation in geriatric COVID-19 patients.

J Clin Lab Anal 2021 Jan 18;35(1):e23654. Epub 2020 Nov 18.

Cardiology Department, China-Japan Union Hospital of Jilin University, Changchun, China.

Background: Geriatric patients with coronavirus disease (COVID-19) are at high risk of developing cardiac injury. Identifying the factors that affect high-sensitivity cardiac troponin I may indicate the cause of cardiac injury in elderly patients, and this could hopefully assist in protecting heart function in this patient population.

Methods: One hundred and eighty inpatients who were admitted for COVID-19 were screened. Patients older than 60 years were included in this study, and the clinical characteristics and laboratory results of the cohort were analyzed. The correlation between cardiac injury and clinical/laboratory variables was statistically analyzed, and further logistic regression was performed to determine how these variables influence cardiac injury in geriatric patients.

Results: Age (p < 0.001) significantly correlated with cardiac injury, whereas sex (p = 0.372) and coexisting diseases did not. Rising procalcitonin (p = 0.001), interleukin-2 receptor (p < 0.001), interleukin 6 (p = 0.001), interleukin 10 (p < 0.001), tumor necrosis factor α (p = 0.001), high-sensitivity C-reactive protein (p = 0.001), D-dimer (p < 0.001), white blood cells (p < 0.001), neutrophils (p = 0.001), declining lymphocytes (p < 0.001), and natural killer cells (p = 0.005) were associated with cardiac injury and showed predictive ability in the multivariate logistic regression.

Conclusion: Our results suggest that age and inflammatory factors influence cardiac injury in elderly patients. Interfering with inflammation in this patient population may potentially confer cardiac protection.
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http://dx.doi.org/10.1002/jcla.23654DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7744922PMC
January 2021

Preparation and application of chitosan biomaterials in dentistry.

Int J Biol Macromol 2021 Jan 14;167:1198-1210. Epub 2020 Nov 14.

National Engineering Research Center for Biomaterials, Sichuan University, 610064 Chengdu, China; College of Biomedical Engineering, Sichuan University, 610064 Chengdu, China. Electronic address:

Chitosan is a biodegradable and biocompatible natural polysaccharide that has a wide range of applications in the field of dentistry due to its functional versatility and ease of access. Recent studies find that chitosan and its derivatives can be embedded in materials for dental adhesives, barrier membranes, bone replacement, tissue regeneration, and antimicrobial agent to better manage oral diseases. In this paper, we provide a comprehensive overview on the preparation, applications, and major breakthroughs of chitosan biomaterials. Furthermore, incorporation of chitosan additives for the modification and improvement of dental materials has been discussed in depth to promote more advanced chitosan-related research in the future.
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http://dx.doi.org/10.1016/j.ijbiomac.2020.11.073DOI Listing
January 2021

Fine-Tuning Protein Self-Organization by Orthogonal Chemo-Optogenetic Tools.

Angew Chem Int Ed Engl 2021 02 7;60(9):4501-4506. Epub 2021 Jan 7.

Max Planck Institute of Biochemistry, Am Klopferspitz 18, 82152, Martinsried, Germany.

A universal gain-of-function approach for the spatiotemporal control of protein activity is highly desirable when reconstituting biological modules in vitro. Here we used orthogonal translation with a photocaged amino acid to map and elucidate molecular mechanisms in the self-organization of the prokaryotic filamentous cell-division protein (FtsZ) that is highly relevant for the assembly of the division ring in bacteria. We masked a tyrosine residue of FtsZ by site-specific incorporation of a photocaged tyrosine analogue. While the mutant still shows self-assembly into filaments, dynamic self-organization into ring patterns can no longer be observed. UV-mediated uncaging revealed that tyrosine 222 is essential for the regulation of the protein's GTPase activity, self-organization, and treadmilling dynamics. Thus, the light-mediated assembly of functional protein modules appears to be a promising minimal-regulation strategy for building up molecular complexity towards a minimal cell.
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http://dx.doi.org/10.1002/anie.202008691DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7986231PMC
February 2021

N-glycosylation of PD-1 promotes binding of camrelizumab.

EMBO Rep 2020 12 15;21(12):e51444. Epub 2020 Oct 15.

Faculty of Health Sciences, University of Macau, Macau SAR, China.

PD-1 is a highly glycosylated inhibitory receptor expressed mainly on T cells. Targeting of PD-1 with monoclonal antibodies (MAbs) to block the interaction with its ligand PD-L1 has been successful for the treatment of multiple tumors. However, polymorphisms at N-glycosylation sites of PD-1 exist in the human population that might affect antibody binding, and dysregulated glycosylation has been observed in the tumor microenvironment. Here, we demonstrate varied N-glycan composition in PD-1, and show that the binding affinity of camrelizumab, a recently approved PD-1-specific MAb, to non-glycosylated PD-1 proteins from E. coli is substantially decreased compared with glycosylated PD-1. The structure of the camrelizumab/PD-1 complex reveals that camrelizumab mainly utilizes its heavy chain to bind to PD-1, while the light chain sterically inhibits the binding of PD-L1 to PD-1. Glycosylation of asparagine 58 (N58) promotes the interaction with camrelizumab, while the efficiency of camrelizumab to inhibit the binding of PD-L1 is substantially reduced for glycosylation-deficient PD-1. These results increase our understanding of how glycosylation affects the activity of PD-1-specific MAbs during immune checkpoint therapy.
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http://dx.doi.org/10.15252/embr.202051444DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7726772PMC
December 2020

3D printed titanium scaffolds with homogeneous diamond-like structures mimicking that of the osteocyte microenvironment and its bone regeneration study.

Biofabrication 2020 Oct 12. Epub 2020 Oct 12.

Department of Physics, Sichuan University, Chengdu, CHINA.

Biofabrication of personalized titanium scaffold mimicking that of the osteocyte microenvironment is challenging due to its complex geometrical cues. The effect of scaffolds geometrical cues and implantation sites on osteogenesis is still not clear. In this study, personalized titanium scaffolds with homogeneous diamond-like structures mimicking that of the osteocyte microenvironment were precisely designed and fabricated by selected laser melting method. The effects of different geometric cues, including porosity, pore sizes and interconnection properties, on cellular behavior were investigated. Biomimetic mechanical properties of porous titanium alloy scaffold were predesigned and simulated by finite element analysis. In vitro experiment revealed that homogeneous diamond-like structures mimicking that of the osteocyte microenvironment triggered osteocyte adhesion and migration behavior. Typical implantation sites, including rabbit femur, beagle femur, and beagle skull, were used to study the implantation sites effects on bone regeneration. In vivo experimental results indicated that different implantation sites showed significant differences. This study helps to understand the scaffolds geometrical microenvironment and implantation sites effects on osteogenesis mechanism. And it is beneficial to the development of bone implants with better bone regeneration ability.
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http://dx.doi.org/10.1088/1758-5090/abc060DOI Listing
October 2020

Expanding the Scope of Orthogonal Translation with Pyrrolysyl-tRNA Synthetases Dedicated to Aromatic Amino Acids.

Molecules 2020 Sep 25;25(19). Epub 2020 Sep 25.

Institut für Chemie, Technische Universität Berlin, Müller-Breslau-Straße 10, 10623 Berlin, Germany.

In protein engineering and synthetic biology, pyrrolysyl-tRNA synthetase (PylRS), with its cognate tRNA, is one of the most popular tools for site-specific incorporation of non-canonical amino acids (ncAAs). Numerous orthogonal pairs based on engineered PylRS variants have been developed during the last decade, enabling a substantial genetic code expansion, mainly with aliphatic pyrrolysine analogs. However, comparatively less progress has been made to expand the substrate range of PylRS towards aromatic amino acid residues. Therefore, we set to further expand the substrate scope of orthogonal translation by a semi-rational approach; redesigning the PylRS efficiency. Based on the randomization of residues from the binding pocket and tRNA binding domain, we identify three positions (V401, W417 and S193) crucial for ncAA specificity and enzyme activity. Their systematic mutagenesis enabled us to generate PylRS variants dedicated to tryptophan (such as β-(1-Azulenyl)-l-alanine or 1-methyl-l-tryptophan) and tyrosine (mainly halogenated) analogs. Moreover, our strategy also significantly improves the orthogonal translation efficiency with the previously activated analog 3-benzothienyl-l-alanine. Our study revealed the engineering of both first shell and distant residues to modify substrate specificity as an important strategy to further expand our ability to discover and recruit new ncAAs for orthogonal translation.
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http://dx.doi.org/10.3390/molecules25194418DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7582959PMC
September 2020

Review of Plastic Surgery Biomaterials and Current Progress in Their 3D Manufacturing Technology.

Materials (Basel) 2020 Sep 16;13(18). Epub 2020 Sep 16.

Department of Palliative Care, West China School of Public Health and West China Fourth, Sichuan University, Chengdu 610041, China.

Plastic surgery is a broad field, including maxillofacial surgery, skin flaps and grafts, liposuction and body contouring, breast surgery, and facial cosmetic procedures. Due to the requirements of plastic surgery for the biological safety of materials, biomaterials are widely used because of its superior biocompatibility and biodegradability. Currently, there are many kinds of biomaterials clinically used in plastic surgery and their applications are diverse. Moreover, with the rise of three-dimensional printing technology in recent years, the macroscopically more precise and personalized bio-scaffolding materials with microporous structure have made good progress, which is thought to bring new development to biomaterials. Therefore, in this paper, we reviewed the plastic surgery biomaterials and current progress in their 3D manufacturing technology.
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http://dx.doi.org/10.3390/ma13184108DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7560273PMC
September 2020

Arbuscular mycorrhizal fungal diversity in soils underlying moss biocrusts in coal mining subsidence areas.

Environ Sci Pollut Res Int 2021 Jan 12;28(3):3484-3493. Epub 2020 Sep 12.

State Key Laboratory of Coal Resources and Safe Mining, China University of Mining and Technology (Beijing), Beijing, 100083, China.

The potentially symbiotic mycorrhizal associations dominated by arbuscular mycorrhizal (AM) fungi have become a new topic in bioremediation research in response to global change. Biological soil crusts (biocrusts) play an important role in arid and semi-arid ecosystems. However, AM fungal diversity in the soils underlying moss biocrusts in coal mining subsidence areas remains poorly understood. Here, samples of the soil underlying moss biocrusts in an area inoculated with an AM fungus (AM-BS) and an uninoculated area (CK-BS) plus soil samples from an uninoculated bare area (CK-NBS) were collected from the subsidence area of Shendong Daliuta mine at Yulin, northwest China. AM fungal community diversity indices were maximum in AM-BS, intermediate in CK-BS, and minimum in CK-NBS (P < 0.05). In addition, redundancy analysis (RDA) indicates that the importance of moss biocrust to soil properties followed the sequence: soil water content (SWC) > glomalin-related soil protein (TG) > available phosphorus (Olsen-P) > soil organic matter (SOM) > easily extractable glomalin-related soil protein (EEG) > pH > available nitrogen (alkali-N). SWC, alkali-N, Olsen-P, and SOM were significantly related to the abundance of Glomus and Claroideoglomus, and TG, EEG, and pH were positively related to Diversisipora. In summary, inoculation with the exotic AM fungus and moss biocrust cover created a eutrophic microhabitat for AM fungi in the soils underlying moss biocrusts in the coal mining subsidence area.
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http://dx.doi.org/10.1007/s11356-020-10726-yDOI Listing
January 2021

High-throughput and Deep-proteome Profiling by 16-plex Tandem Mass Tag Labeling Coupled with Two-dimensional Chromatography and Mass Spectrometry.

J Vis Exp 2020 08 18(162). Epub 2020 Aug 18.

Departments of Structural Biology and Developmental Neurobiology, St. Jude Children's Research Hospital; Center for Proteomics and Metabolomics, St. Jude Children's Research Hospital;

Isobaric tandem mass tag (TMT) labeling is widely used in proteomics because of its high multiplexing capacity and deep proteome coverage. Recently, an expanded 16-plex TMT method has been introduced, which further increases the throughput of proteomic studies. In this manuscript, we present an optimized protocol for 16-plex TMT-based deep-proteome profiling, including protein sample preparation, enzymatic digestion, TMT labeling reaction, two-dimensional reverse-phase liquid chromatography (LC/LC) fractionation, tandem mass spectrometry (MS/MS), and computational data processing. The crucial quality control steps and improvements in the process specific for the 16-plex TMT analysis are highlighted. This multiplexed process offers a powerful tool for profiling a variety of complex samples such as cells, tissues, and clinical specimens. More than 10,000 proteins and posttranslational modifications such as phosphorylation, methylation, acetylation, and ubiquitination in highly complex biological samples from up to 16 different samples can be quantified in a single experiment, providing a potent tool for basic and clinical research.
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http://dx.doi.org/10.3791/61684DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7752892PMC
August 2020

Both Boceprevir and GC376 efficaciously inhibit SARS-CoV-2 by targeting its main protease.

Nat Commun 2020 09 4;11(1):4417. Epub 2020 Sep 4.

CAS Key Laboratory of Pathogenic Microbiology and Immunology, Institute of Microbiology, Chinese Academy of Sciences, 100101, Beijing, China.

COVID-19 was declared a pandemic on March 11 by WHO, due to its great threat to global public health. The coronavirus main protease (M, also called 3CLpro) is essential for processing and maturation of the viral polyprotein, therefore recognized as an attractive drug target. Here we show that a clinically approved anti-HCV drug, Boceprevir, and a pre-clinical inhibitor against feline infectious peritonitis (corona) virus (FIPV), GC376, both efficaciously inhibit SARS-CoV-2 in Vero cells by targeting M. Moreover, combined application of GC376 with Remdesivir, a nucleotide analogue that inhibits viral RNA dependent RNA polymerase (RdRp), results in sterilizing additive effect. Further structural analysis reveals binding of both inhibitors to the catalytically active side of SARS-CoV-2 protease M as main mechanism of inhibition. Our findings may provide critical information for the optimization and design of more potent inhibitors against the emerging SARS-CoV-2 virus.
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http://dx.doi.org/10.1038/s41467-020-18233-xDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7474075PMC
September 2020

Micheliolide suppresses the viability, migration and invasion of U251MG cells via the NF-κB signaling pathway.

Oncol Lett 2020 Oct 29;20(4):67. Epub 2020 Jul 29.

Department of Neurosurgery, The Affiliated Renhe Hospital, China Three Gorges University, Yichang, Hubei 443000, P.R. China.

Micheliolide (MCL), a sesquiterpene lactone isolated from and , has been used previously to inhibit the NF-κB signaling pathway. MCL has exerted various therapeutic effects in numerous types of disease, such as inflammatory and cancer. However, to the best of our knowledge, its underlying anticancer mechanism remains to be understood. The present study aimed to investigate the effects of MCL on human glioma U251MG cells and to determine the potential anticancer mechanism of action of MCL. From Cell Counting Kit-8, colony formation assay, apoptosis assay and Confocal immunofluorescence imaging analysis, the results revealed that MCL significantly inhibited cell viability and induced cell apoptosis via activation of the cytochrome caspase-dependent apoptotic pathway. In addition, MCL also suppressed cell invasion and metastasis via the wound healing and Transwell invasion assays. Furthermore, western blot and reverse transcription PCR analyses demonstrated that MCL significantly downregulated cyclooxygenase-2 (COX-2) expression levels, which may have partially occurred through the inactivation of the NF-κB signaling pathway. In conclusion, the results of the present study indicated that MCL may inhibit glioma carcinoma growth by downregulating the NF-κB/COX-2 signaling pathway, which suggested that MCL may be a novel and alternative antitumor agent for the treatment of human glioma carcinoma.
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http://dx.doi.org/10.3892/ol.2020.11928DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7436293PMC
October 2020

Revisiting right anterior oblique projections for the triangle of Koch: implications from computed tomography.

BMC Cardiovasc Disord 2020 08 24;20(1):383. Epub 2020 Aug 24.

Cardiology Department, China-Japan Union Hospital of Jilin University, 126 Xiantai Street, Changchun, Jilin Province, 130033, China.

Background: Variability in the anatomy and orientation of the triangle of Koch (TK) complicates ablation procedures involving the atrioventricular (AV) node. We used CT angiography (CTA) to assess the anatomical TK orientation, the CS ostium direction, and the relationship between the two, and we validated an individualized CS-guided projection during ablation procedures.

Methods: In 104 patients without structural heart disease undergoing computed tomography (CT) angiography, TK orientations were determined in relation to the coronary sinus ostium (CSo) as well as two standard right anterior oblique (RAO) projection angles (30 and 45) commonly used in ablation procedures.

Results: A CS-guided RAO projection (RAOCS) was shown to best track the orientation of the TK compared to RAO30° and 45°, with TK orientation strongly correlating with the CSo direction (r = 0.86, P < 0.001). In addition, the mean relative difference between the angle of the CSo and TK orientation was 5.54 ± 0.48°, consistent with a reduction in the degree of image shortening compared to traditional RAOs. Moreover, in vivo validation following ablation revealed that using a CS-guided projection limited the degree of on-screen image shortening compared to both the RAO30° and 45° in 25 patients with catheter ablation procedures.

Conclusion: In hearts with a normal structure, the CSo direction offers a reliable predictor of the TK orientation which can be used to guide the projection of the TK during ablation procedures.
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http://dx.doi.org/10.1186/s12872-020-01632-9DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7446209PMC
August 2020

Local anaesthesia for surgical extraction of mandibular third molars: a systematic review and network meta-analysis.

Clin Oral Investig 2020 Nov 24;24(11):3781-3800. Epub 2020 Aug 24.

State Key Laboratory of Oral Diseases, Department of Conservative Dentistry and Endodontics, West China Hospital of Stomatology, Sichuan University, Chengdu, 610041, China.

Objectives: Pain management for the extraction of the mandibular third molar is a challenge as compelling evidence in comparative anaesthetics is currently lacking.

Materials And Methods: Thorough literature searches took place in PubMed, ScienceDirect, CENTRAL, Embase, Web of Science, CBM, and CNKI. Thirty-three trials were meta-analysed using a Bayesian statistical approach within the random-effects model. Grading of Recommendations Assessment, Development, and Evaluation was performed to determine the overall quality of evidence across all comparisons.

Results: In terms of success rate, an inferior alveolar nerve block (IANB) injection of 2% lidocaine with epinephrine was less effective than a combined injection of buccal infiltration (BI) and lingual infiltration (LI) with a 4% articaine (RR = 0.85 [0.75, 0.96], P = 0.611). According to visual analogue scale (VAS), 2% lidocaine-IANB with epinephrine caused higher VAS scores than 4% articaine-IANB with epinephrine (MD = 0.84 [0.28, 1.40], P = 0.057), whereas 0.5% levobupivacaine-IANB showed lower scores than 2% lidocaine-IANB (MD = - 1.62 [- 2.97, - 0.28], P = 0.045). Also, 2% lidocaine-IANB with epinephrine presented longer latency than both 4% articaine-IANB with epinephrine (MD = 39.44 [16.97, 61.90], P < 0.001) and 4% articaine-BI + LI with epinephrine (MD = 164.41 [16.23, 312.58], P < 0.001); 4% articaine-IANB with epinephrine produced shorter latency than 0.5% bupivacaine-IANB with epinephrine (MD = - 42.92 [- 70.28, - 15.56], P = 0.106); 0.75% ropivacaine-IANB caused shorter onset of action compared with 2% lidocaine-IANB (MD = - 40.88 [- 65.50, - 16.26], P < 0.001). In addition, 2% lidocaine-IANB with epinephrine produced significantly shorter duration than both 4% articaine-IANB with epinephrine (MD = - 47.33 [- 57.88, - 36.77], P = 0.265) and 2% mepivacaine-IANB with epinephrine (MD = - 10.01 [- 19.59, - 0.44], P = 0.769). The duration of action triggered by 4% articaine-IANB with epinephrine was shorter compared with 0.5% bupivacaine-IANB with epinephrine (MD = - 64.17 [- 74.65, - 53.69], P = 0.926). Both 0.5% levobupivacaine-IANB and 0.75% ropivacaine-IANB produced longer duration of action than 2% lidocaine-IANB (MD = 333.70 [267.33, 400.07], P < 0.001) and (MD = 288.01 [287.67, 288.34], P = 0.634, respectively).

Conclusions: The network meta-analysis demonstrated that the intraosseous injection of 4% articaine with epinephrine had the most noteworthy success rate. However, the combination of BI and LI of 4% articaine with epinephrine, and IANB of 0.5% bupivacaine were, according to a VAS, the most effective. It should be noted that a rapid onset of action was produced by BI combined with LI of 4% articaine with epinephrine and IANB of 2% mepivacaine with epinephrine, while the most prolonged duration of action was generated by IANB of 0.5% levobupivacaine or 0.5% bupivacaine.

Clinical Relevance: For a better understanding of local anaesthesia for the extraction of the third molar, our study was aimed to provide evidence to guide better dental practices in pain management for clinicians.
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http://dx.doi.org/10.1007/s00784-020-03490-3DOI Listing
November 2020

Death, discharge and arrhythmias among patients with COVID-19 and cardiac injury.

CMAJ 2020 07 24;192(28):E791-E798. Epub 2020 Jun 24.

Department of Cardiology (Si, Du, Sun, Liu, Yang), The Third Hospital of Jilin University, Jilin Provincial Molecular Biology Research Center for Precision Medicine of Major Cardiovascular Disease, Changchun, China; Department of Ultrasound (Ni), The Third Hospital of Jilin University, Changchun, China; Institute of Organ Transplantation (Yang), Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China; Intensive Care Unit (Jiang), The Third Hospital of Jilin University, Changchun, China; The Hull Family Laboratory (Massé, J. Nanthakumar, Bhaskaran, K. Nanthakumar), Peter Munk Cardiac Centre, Toronto General Hospital, University of Toronto, Toronto, Ont.; Department of Epidemiology and Biostatistics (Jin), School of Public Health, Jilin University, Changchun, Jilin, China

Background: Cardiac injury is common in severe coronavirus disease 2019 (COVID-19) and is associated with poor outcomes. We aimed to study predictors of in-hospital death, characteristics of arrhythmias and the effects of QT-prolonging therapy in patients with cardiac injury.

Methods: We conducted a retrospective cohort study involving patients with severe COVID-19 who were admitted to Tongji Hospital in Wuhan, China, between Jan. 29 and Mar. 8, 2020. Among patients who had cardiac injury, which we defined as an elevated level of cardiac troponin I (cTnI), we identified demographic and clinical characteristics associated with mortality and need for invasive ventilation.

Results: Among 1284 patients with severe COVID-19, 1159 had a cTnI level measured on admission to hospital, of whom 170 (14.7%) had results that showed cardiac injury. We found that mortality was markedly higher in patients with cardiac injury (71.2% v. 6.6%, < 0.001). We determined that initial cTnI (per 10-fold increase, hazard ratio [HR] 1.32, 95% confidence interval [CI] 1.06-1.66) and peak cTnI level during illness (per 10-fold increase, HR 1.70, 95% CI 1.38-2.10) were associated with poor survival. Peak cTnI was also associated with the need for invasive ventilation (odds ratio 3.02, 95% CI 1.92-4.98). We found arrhythmias in 44 of the 170 patients with cardiac injury (25.9%), including 6 patients with ventricular tachycardia or fibrillation, all of whom died. We determined that patients who received QT-prolonging drugs had longer QTc intervals than those who did not receive them (difference in medians, 45 ms, = 0.01), but such treatment was not independently associated with mortality (HR 1.04, 95% CI 0.69-1.57).

Interpretation: We found that in patients with COVID-19 and cardiac injury, initial and peak cTnI levels were associated with poor survival, and peak cTnI was a predictor of need for invasive ventilation. Patients with COVID-19 warrant assessment for cardiac injury and monitoring, especially if therapy that can prolong repolarization is started.

Trial Registration: Chinese Clinical Trial Registry, No. ChiCTR2000031301.
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http://dx.doi.org/10.1503/cmaj.200879DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7828865PMC
July 2020

Efficacy and Safety of Recanalization Therapy for Acute Ischemic Stroke With Large Vessel Occlusion: A Systematic Review.

Stroke 2020 07 3;51(7):2026-2035. Epub 2020 Jun 3.

Cerebrovascular Diseases Research Institute, Xuanwu Hospital (C.W., W.Z., X.J.), Capital Medical University, Beijing, China.

Background And Purpose: The optimal recanalization strategy for acute ischemic stroke with large vessel occlusion continues to be an area of active interest. Network meta-analysis can provide insight when direct comparative evidence is lacking.

Methods: A systematic review of the literature using PubMed, Embase, the Cochrane Central Register of Controlled Trials, and SinoMed was performed, and a search was conducted for clinical trials on ClinicalTrials.gov, the World Health Organization International Clinical Trials Registry Platform, and StrokeCenter.org. Four independent reviewers conducted the study selection, data abstraction, and quality assessments.

Results: The literature review identified 17 trials including 3236 patients and 8 ongoing clinical trials. Sample sizes ranged from 7 to 656 participants. Intravenous thrombolysis (IVT) was the most common intervention, followed by IVT plus mechanical thrombectomy (MT), IVT plus intraarterial thrombolysis, intraarterial thrombolysis alone, and MT alone. In the pooled network meta-analysis, IVT+MT was associated with a higher rate of independent functioning. In contrast, IVT was ranked as the most ineffective treatment strategy with respect to neurological functions, while direct MT was ranked as the least safe intervention with respect to all-cause mortality. Also, irrespective of assessment tools, endovascular treatment plus IVT led to higher successful recanalization rate than thrombolysis alone.

Conclusions: Compared with other recanalization treatments, IVT+MT seems to be the most effective strategy, without increasing detrimental effects, for thrombolysis-eligible patients with large vessel occlusion-acute ischemic stroke. To improve the current evidentiary basis for recanalization treatment, future trials and real-world studies are warranted and should use unified definitions of symptomatic intracranial hemorrhage and recanalization.
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http://dx.doi.org/10.1161/STROKEAHA.119.028624DOI Listing
July 2020

Structural basis of HCoV-19 fusion core and an effective inhibition peptide against virus entry.

Emerg Microbes Infect 2020 Dec;9(1):1238-1241

CAS Key Laboratory of Pathogenic Microbiology and Immunology, Institute of Microbiology, Chinese Academy of Sciences (CAS), Beijing, People's Republic of China.

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http://dx.doi.org/10.1080/22221751.2020.1770631DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7448930PMC
December 2020

Efficacy of interventions for amblyopia: a systematic review and network meta-analysis.

BMC Ophthalmol 2020 May 25;20(1):203. Epub 2020 May 25.

Department of Evidence-Based Medicine and Clinical Epidemiology, West China Hospital, Sichuan University, No.37 Guoxue Alley, Chengdu, 610041, China.

Background: Many treatments are currently available for amblyopic patients; although, the comparative efficacy of these therapies is unclear. We conducted a systematic review and network meta-analysis (NMA) to establish the relative efficacy of these treatments for amblyopia.

Methods: Electronic databases (MEDLINE, EMBASE, Cochrane Library) were systematically searched from inception to Sep. 2019. Only Randomized clinical trials comparing any two or three of the following treatments were included: refractive correction (spectacles alone), patching of 2 h per day (patch 2H), patch 6H, patch 12H, patch 2H + near activities (N), patch 2H + distant activities (D), atropine (Atr) daily, Atr weekly, Atr weekly + plano lens over the sound eye (Plano), optical penalization and binocular therapy. The reviewers independently extracted the data according to the PRISMA guidelines; assessed study quality by Cochrane risk-of-bias tool for randomized trials. The primary outcome measure was the change in best-corrected visual acuity (BCVA) expressed as log MAR lines. Direct comparisons and a Bayesian meta-analysis were performed to synthesize data.

Results: Twenty-three studies with 3279 patients were included. In the network meta-analysis, optical penalization was the least effective of all the treatments for the change of visual acuity, spectacles (mean difference [MD], 2.9 Log MAR lines; 95% credibility interval [CrI], 1.8-4.0), patch 2H (MD, 3.3; 95% CrI, 2.3-4.3), patch 6H (MD, 3.6; 95% CrI, 2.6-4.6), patch 12H (MD, 3.4; 95% CrI, 2.3-4.5), patch 2H + N (MD, 3.7; 95% CrI, 2.5-5.0), patch 2H + D (MD, 3.5; 95% CrI, 2.1-5.0), Atr daily (MD, 3.2; 95% CrI, 2.2-4.3), Atr weekly (MD, 3.2; 95% CrI, 2.2-4.3), Atr weekly + Plano (MD, 3.7; 95% CrI, 2.7-4.7), binocular therapy (MD, 3.1; 95% CrI, 2.0-4.2). The patch 6H and patch 2H + N were better than spectacles ([MD, 0.73; 95% Crl, 0.10-1.40]; [MD, 0.84; 95% CrI, 0.19-1.50]).

Conclusions: The NMA indicated that the efficacy of the most of the examined treatment modalities for amblyopia were comparable, with no significant difference. Further high quality randomized controlled trials are required to determine their efficacy and acceptability.

Systematic Review Registration: CRD42019119843.
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http://dx.doi.org/10.1186/s12886-020-01442-9DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7249307PMC
May 2020

27-Plex Tandem Mass Tag Mass Spectrometry for Profiling Brain Proteome in Alzheimer's Disease.

Anal Chem 2020 05 7;92(10):7162-7170. Epub 2020 May 7.

Departments of Structural Biology and Developmental Neurobiology, St. Jude Children's Research Hospital, Memphis, Tennessee 38105, United States.

Multiplexed isobaric labeling methods, such as tandem mass tags (TMT), remarkably improve the throughput of quantitative mass spectrometry. Here, we present a 27-plex TMT method coupled with two-dimensional liquid chromatography (LC/LC) for extensive peptide fractionation and high-resolution tandem mass spectrometry (MS/MS) for peptide quantification and then apply the method to profile the complex human brain proteome of Alzheimer's disease (AD). The 27-plex method combines multiplexed capacities of the 11-plex and the 16-plex TMT, as the peptides labeled by the two TMT sets display different mass and hydrophobicity, which can be well separated in LC-MS/MS. We first systematically optimized the protocol for the newly developed 16-plex TMT, including labeling reaction, desalting, and MS conditions, and then directly compared the 11-plex and 16-plex methods by analyzing the same human AD samples. Both methods yielded similar proteome coverage, analyzing >100 000 peptides in >10 000 human proteins. Furthermore, the 11-plex and 16-plex samples were mixed for a 27-plex assay, resulting in more than 8000 protein measurements within the same MS time. The 27-plex results are highly consistent with those of the individual 11-plex and 16-plex TMT analyses. We also used these proteomics data sets to compare the AD brain with the nondementia controls, discovering major AD-related proteins and revealing numerous novel protein alterations enriched in the pathways of amyloidosis, immunity, mitochondrial, and synaptic functions. Overall, our data strongly demonstrate that this new 27-plex strategy is highly feasible for routine large-scale proteomic analysis.
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http://dx.doi.org/10.1021/acs.analchem.0c00655DOI Listing
May 2020

Dual modulation of crystallinity and macro-/microstructures of 3D printed porous titanium implants to enhance stability and osseointegration.

J Mater Chem B 2019 05 4;7(17):2865-2877. Epub 2019 Apr 4.

School of Manufacturing Science and Engineering, Sichuan University, Chengdu 610065, China.

The macro architecture and micro surface topological morphology of implants play essential roles in bone tissue regeneration. 3D printing technology provides enormous advantages for the rapid fabrication of personalized bone tissue repair implants. This study presents a demonstration of dual-modulation (DM) 3D printed porous titanium implants to enhance stability and osseointegration. Titanium implants with the first level of modulation of macro porous architecture and mechanical properties are obtained using macro architecture design and 3D printing fabrication. The first level of modulation achieved scaffolds with a wide range of compressive strengths (36.76-139.97 MPa) when varying the scaffold macro architectures. In the second level of modulation of surface topological morphology, alkali treatment, heat treatments and electrochemical deposition of hydroxyapatite coating were conducted for further regulating the biological function of implants. DM 3D printed scaffolds significantly promoted bone marrow mesenchyme stem cell adhesion and proliferation, indicating good cytocompatibility. In addition, in vivo osseointegration experiments indicated that the DM scaffolds formed better tissue-materials interfaces. New bone formation rates in DM scaffolds are higher than those in conventional 3D printed scaffolds after 6 months of implantation (58.1% versus 36.1%). These results demonstrate that DM scaffolds could enhance early stability and osseointegration. This study may provide new insights into the design, fabrication and post-processing of 3D printed porous titanium implants for various applications in personalized bone tissue regeneration.
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http://dx.doi.org/10.1039/c9tb00093cDOI Listing
May 2019

[Network Meta-analysis of effect of traditional Chinese medicine injections in adjuvant treatment of mycoplasma pneumonia in children].

Zhongguo Zhong Yao Za Zhi 2020 Jan;45(2):425-435

First Teaching Hospital of Tianjin University of Traditional Chinese Medicine Tianjin 300193, China.

To assess the clinical efficacy of traditional Chinese medicine injection for adjuvant treatment of mycoplasma pneumoniae pneumonia in children by network Meta-analysis method. We retrieved CNKI, WanFang, CBM, Cochrane Library, PubMed from the establishment to September 2018. Two reviewers independently screened out literatures, extracted data and assessed the methodological quality of included studies. The data were analyzed by Stata 13.0 software. Totally 89 RCTs were included, involving 8 kinds of traditional Chinese medical injections and 8 936 patients. According to the results of network Meta-analysis, the order by the total effective rate from high to low was Huangqi Injection>Xiyanping Injection>Tanreqing Injection>Compound Danshen Injection>Reduning Injection>Yanhuning Injection>Qingkailing Injection>Xixinnao Injection; the order by cooling time from high to low was Reduning Injection> Yanhuning Injection>Qingkailing Injection>Tanreqing Injection>Huangqi Injection>Xiyanping Injection>Xiexinnao Injection>Compound Danshen Injection; the order by the cough disappeared time from high to low was Compound Danshen Injection>Qingkailing Injection>Xiyanping Injection>Huangqi Injection>Yanhuning Injection>Reduning Injection>Tanreqing Injection>Xixinnao Injection; the order by the rales disappearing time from high to low was Qingkailing Injection>Yanhuning Injection>Reduning Injection>Huangqi Injection>Tanreqing Injection>Xiyanping Injection>Xixinnao Injection. The results show that traditional Chinese medicine injection has a significant clinical efficacy in the adjuvant treatment of various symptoms of mycoplasma pneumoniae pneumonia in children. Due to the small sample size, more studies are required to verify the strength of evidence.
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http://dx.doi.org/10.19540/j.cnki.cjcmm.20190826.501DOI Listing
January 2020

High-Strength Albumin Hydrogels With Hybrid Cross-Linking.

Front Chem 2020 25;8:106. Epub 2020 Feb 25.

School of Materials Science and Engineering, Henan Polytechnic University, Jiaozuo, China.

Natural protein-based hydrogels possess excellent biocompatibility; however, most of them are weak or brittle. In the present work, high strength hybrid dual-crosslinking BSA gels (BSA DC gels), which have both chemical cross-linking and physical cross-linking, were fabricated by a facile photoreaction-heating process. BSA DC gels showed high transparency (light transmittance of ~90%) and high strength. At optimal conditions, BSA DC gel exhibited high compressive strength (σ) of 37.81 ± 2.61 MPa and tensile strength (σ) of 0.62 ± 0.078 MPa, showing it to be much stronger than physically cross-linked BSA gel (BSA PC gel) and chemically cross-linked BSA gel (BSA CC gel). More importantly, BSA DC gel displayed non-swelling properties while maintaining high strength in DI water, pH = 3.0, and pH = 10.0. Moreover, BSA DC gel also demonstrated large hysteresis, rapid self-recovery, and excellent fatigue resistance properties. It is believed that our BSA DC gel can potentially be applied in biomedical fields.
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http://dx.doi.org/10.3389/fchem.2020.00106DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7052378PMC
February 2020

Human amniotic membrane allograft, a novel treatment for chronic diabetic foot ulcers: A systematic review and meta-analysis of randomised controlled trials.

Int Wound J 2020 Jun 2;17(3):753-764. Epub 2020 Mar 2.

Department of Evidence-Based Medicine and Clinical Epidemiology, West China Hospital, Sichuan University, Chengdu City, Sichuan Province, China.

To evaluate the efficacy and safety of human amniotic membrane (HAM) allograft in treating chronic diabetic foot ulcers (DFUs), a comprehensive search of randomised controlled trials in MEDLINE, EMBASE, PubMed, CENTRAL and Web of Science was conducted to December 7, 2019. Two reviewers independently screened the studies, extracted data, and evaluated the quality of studies. The primary outcome was the proportion of complete healing. The secondary outcomes were mean time to complete healing and adverse events. Statistical analyses were performed using RevMan 5.3. We identified 257 articles, of which 7 articles (465 participants) were included in the meta-analysis. The proportion of complete wound healing in HAM plus standard of care (SOC) group was 3.88 times as high as that in SOC alone (RR: 3.88 [95% CI: 2.34, 6.44]) at 6 weeks, and 2.01 times at 12 weeks (RR: 2.01 [95%CI: 1.45, 2.77]). The intervention group had a significantly shorter time to complete healing (MD: -30.33 days, [95% CI: -37.95, -22.72]). The number needed to treat within 6 weeks was 2.3 ([95% CI: 1.8, 3.1]). No significant difference was shown in adverse events. Results were consistent in a sensitivity analysis. Hence, HAM plus SOC is effective and safe in treating chronic DFUs.
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http://dx.doi.org/10.1111/iwj.13318DOI Listing
June 2020