Publications by authors named "Huan Liu"

1,484 Publications

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Rapid determination of infectious SARS-CoV-2 in PCR-positive samples by SDS-PMA assisted RT-qPCR.

Sci Total Environ 2021 Jul 17;797:149085. Epub 2021 Jul 17.

CAS Key Laboratory of Emerging Pathogens and Biosafety, Centre for Biosafety Mega-Sciences, Wuhan Institute of Virology, Chinese Academy of Sciences, Wuhan 430071, China; University of Chinese Academy of Sciences, Beijing 100049, China. Electronic address:

The ongoing COVID-19 pandemic has generated a global health crisis that needs well management of not only patients but also environments to reduce SARS-CoV-2 transmission. The gold standard RT-qPCR method is sensitive and rapid to detect SARS-CoV-2 nucleic acid, but does not answer if PCR-positive samples contain infectious virions. To circumvent this problem, we report an SDS-propidium monoazide (PMA) assisted RT-qPCR method that enables rapid discrimination of live and dead SARS-CoV-2 within 3 h. PMA, a photo-reactive dye, can react with viral RNA released or inside inactivated SARS-CoV-2 virions under assistance of 0.005% SDS, but not viral RNA inside live virions. Formation of PMA-RNA conjugates prevents PCR amplification, leaving only infectious virions to be detected. Under optimum conditions, RT-qPCR detection of heat-inactivated SARS-CoV-2 resulted in larger than 9 Ct value differences between PMA-treated and PMA-free groups, while less than 0.5 Ct differences were observed in the detection of infectious SARS-CoV-2 ranging from 20 to 5148 viral particles. Using a cutoff Ct difference of 8.6, this method could differentiate as low as 8 PFU live viruses in the mixtures of live and heat-inactivated virions. Further experiments showed that this method could successfully monitor the natural inactivation process of SARS-CoV-2 on plastic surfaces during storage with comparable results to the gold standard plaque assay. We believe that the culture-free method established here could be used for rapid and convenient determination of infectious SARS-CoV-2 virions in PCR-positive samples, which will facilitate better control of SARS-CoV-2 transmission.
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http://dx.doi.org/10.1016/j.scitotenv.2021.149085DOI Listing
July 2021

An integrated geospatial correlation analysis and human health risk assessment approach for investigating abandoned industrial sites.

J Environ Manage 2021 Sep 7;293:112891. Epub 2021 Jun 7.

School of Engineering, University of British Columbia, Okanagan, 3333 University Way, Kelowna, BC, V1V 1V7, Canada. Electronic address:

An integrated geospatial correlation analysis (GCA)-human health risk assessment (HHRA) approach was developed to investigate abandoned industrial sites featured by heterogeneous contamination data. Critical areas of high health risk concerns can be prioritized for remediation using the integrated approach. An abandoned chemical complex site in Hubei, China was investigated as a case study. GCA and HHRA were performed using soil and groundwater sampling data collected in 2016 and 2019. Benzene, chlorobenzene, dichlorobenzenes, 2-nitrochlorobenzene, and α-hexachlorocyclohexane were determined to be critical contaminants in soil. The 2019 sampling data revealed new contaminated locations that were not found in the 2016 sampling campaign. High concentrations (89.81-386.55 mg/L) of vinyl chloride were also found in groundwater samples. Several critical location clusters of high concentrations of dichlorobenzenes, chlorobenzene, and α-hexachlorocyclohexane were found within the site according to the GCA outcomes. These contaminants could pose significant cancer and non-cancer risks to onsite workers. The critical areas were ranked according to cancer and non-cancer risks estimated by HHRA, respectively, for informed remediation planning. Among the critical contaminants, α-hexachlorocyclohexane, 2-nitrochlorobenzene, and 1,4-dichlorobenzene in soil, as well as vinyl chloride in groundwater, contributed a predominant part to the total health risk. The integrated approach can be used to assess the contamination of other similar abandoned industrial complex sites.
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http://dx.doi.org/10.1016/j.jenvman.2021.112891DOI Listing
September 2021

Lattice Defect Engineering Enables Performance-Enhanced MoS Photodetection through a Paraelectric BaTiO Dielectric.

ACS Nano 2021 Jul 20. Epub 2021 Jul 20.

Key Laboratory of LCR Materials and Devices of Yunnan Province National Centre for International Research on Photoelectric and Energy Materials School of Materials and Energy Yunnan University Kunming 650500, P.R. China.

Carrier mobility and density are intrinsically important in nanophoto/electronic devices. High-dielectric-constant coupled polarization-field gate ferroelectrics are frequently studied and partially capable in achieving large-scale tuning of photoresponse, but their light absorption and carrier density seem generally ineffective. This raises questions about whether a similarly high-dielectric-constant paraelectric gate dielectric could enable tuning and how the principles involved could be established. In this study, by deliberately introducing lattice defects in high-dielectric-constant paraelectric, cubic BaTiO (c-BTO) was explored to fabricate MoS photodetectors with ultrahigh detection ability and outstanding field-effect traits. An organic-metal-based spin-coating cum annealing method was used for the c-BTO synthesis, with an optimized thickness (300 nm), by introducing lattice defects properly but maintaining a large dielectric constant (55 at 1k Hz) and low dielectric loss (0.06 at 1k Hz), which renders the enhanced visible-light region absorption. As a result of the synergistically enhanced mobility and photoabsorption, the MoS/BTO FET exhibits promising merits, for example, on/off ratio, subthreshold swing, and mobilities for high-performance photodetectors with excellent responsivity (600 AW) and detectivity (1.25 × 10 Jones). Thus, this work facilitates the establishment of a lattice defect induced sub-bandgap absorption landmap for synergistically enhanced photoresponse for high-performance photodetector exploration.
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http://dx.doi.org/10.1021/acsnano.1c03402DOI Listing
July 2021

Copper exposure induces hepatic G0/G1 cell-cycle arrest through suppressing the Ras/PI3K/Akt signaling pathway in mice.

Ecotoxicol Environ Saf 2021 Jul 14;222:112518. Epub 2021 Jul 14.

College of Veterinary Medicine, Sichuan Agricultural University, Wenjiang, Chengdu 611130, China.

Copper (Cu), as a common chemical contaminant in environment, is known to be toxic at high concentrations. The current research demonstrates the effects of copper upon hepatocyte cell-cycle progression (CCP) in mice. Institute of cancer research (ICR) mice (n = 240) at an age of four weeks were divided randomly into groups treated with different doses of Cu (0, 4, 8, and 16 mg/kg) for 21 and 42 days. Results showed that high Cu exposure caused hepatocellular G0/G1 cell-cycle arrest (CCA) and reduced cell proportion in the G2/M phase. G0/G1 CCA occurred with down-regulation (p < 0.05) of Ras, p-PI3K (Tyr458), p-Akt (Thr308), p-forkhead box O3 (FOXO3A) (Ser253), p-glycogen synthase kinase 3-β (GSK3-β) (Ser9), murine double minute 2 (MDM2) protein, and mRNA expression levels, and up-regulation (p < 0.05) of PTEN, p-p53 (Ser15), p27, p21 protein, and mRNA expression levels, which subsequently suppressed (p < 0.05) the protein and mRNA expression levels of CDK2/4 and cyclin E/D. These results indicate that Cu exposure suppresses the Ras/PI3K/Akt signaling pathway to reduce the level of CDK2/4 and cyclin E/D, which are essential for the G1-S transition, and finally causes hepatocytes G0/G1 CCA.
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http://dx.doi.org/10.1016/j.ecoenv.2021.112518DOI Listing
July 2021

Health-Related Quality of Life after Extensive Aortic Replacement.

Semin Thorac Cardiovasc Surg 2021 Jul 13. Epub 2021 Jul 13.

Department of Thoracic and Cardiovascular Surgery, Cleveland Clinic, Cleveland, OH, USA. Electronic address:

To assess and compare patient-reported long-term health-related quality of life (HRQoL) after combined proximal aortic (arch ± ascending, root) and distal aortic (descending thoracic ± abdominal) replacement using open vs. multimodal/endovascular (hybrid) approaches. From 2010 to 2016, 146 adults underwent single- or multi-stage aortic arch plus descending thoracic aorta replacement, 31 open and 115 hybrid. The two surgical approach groups had similar preoperative characteristics and extent of surgery. Cross-sectional follow-up revealed 49 deaths (7 open, 42 hybrid). Of the 97 survivors, 72 (74%) responded to the Patient-Reported Outcomes Measurement Information System (PROMIS) Global-10 survey (18 open, 54 hybrid) a median 6.2 years (15th, 85th percentiles: 3.1, 7.9) after their last aortic surgery. Predictors of HRQoL scores were identified by random forest regression. Overall physical HRQoL T-score was lower than that of population norms (46 vs. 50, P<.0001); mental HRQoL T-score was similar (50 vs. 50, P>.9). Neither T-score was significantly different according to surgical approach (P≥.3). Greater number of postoperative complications and history of chronic obstructive pulmonary disease were the most important predictors of lower physical HRQoL, and prior myocardial infarction was the most important predictor of lower mental HRQoL. Although extensive aortic replacement had a small long-term effect on patient-reported physical HRQoL, both physical and mental HRQoL can be preserved in survivors with both surgical approaches. Surgeons should recommend the approach they believe will yield the best long-term survival, but lifelong follow-up is crucial, and patients should understand that they may require multiple operations.
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http://dx.doi.org/10.1053/j.semtcvs.2021.07.006DOI Listing
July 2021

Improved Characterization of the Pharmacokinetics of Acalabrutinib and its Pharmacologically Active Metabolite, ACP-5862, in Patients With B-Cell Malignancies and in Healthy Subjects Using a Population Pharmacokinetic Approach.

Br J Clin Pharmacol 2021 Jul 15. Epub 2021 Jul 15.

Clinical Pharmacology & Quantitative Pharmacology (CPQP), Clinical Pharmacology and Safety Sciences, R&D, AstraZeneca, Cambridge, UK.

This analysis aimed to describe the pharmacokinetics (PK) of acalabrutinib and its active metabolite, ACP-5862. A total of 8935 acalabrutinib samples from 712 subjects and 2394 ACP-5862 samples from 304 subjects from 12 clinical studies in patients with B-cell malignancies and healthy subjects were analyzed by non-linear mixed-effects modelling. Acalabrutinib PK was characterized by a two-compartment model with first-order elimination. The large variability in absorption was adequately described by transit compartment chain and first-order absorption, with between-occasion variability on the mean transit time and relative bioavailability. The PK of ACP-5862 was characterized by a two-compartment model with first-order elimination, and the formation rate was defined as the acalabrutinib clearance multiplied by the fraction metabolized. Health status, Eastern Cooperative Oncology Group performance status, and co-administration of proton pump inhibitors were significant covariates. However, none of the investigated covariates led to clinically meaningful changes in exposure, supporting a flat dosing of acalabrutinib.
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http://dx.doi.org/10.1111/bcp.14988DOI Listing
July 2021

A Semi-automated System of Luciferase Immunoprecipitation Assay for Rapid and Easy Detection of Antibody.

J Clin Microbiol 2021 Jul 14:JCM0099021. Epub 2021 Jul 14.

CAS Key Laboratory of Special Pathogens and Biosafety, Center for Biosafety Mega-Science, Wuhan Institute of Virology, Chinese Academy of Sciences, Wuhan 430071, China.

African swine fever (ASF) is a highly contagious viral disease of domestic pigs and wild boars. For the disease surveillance and control, we developed a rapid and easy luciferase immunoprecipitation assay (MB-LIPS) to detect ASF virus (ASFV) antibody. The MB-LIPS is based on magnetic beads modified with protein A/G and the recombinant fusion protein of ASFV p30 and luciferase, where p30 functioned as the recognition element and the luciferase as the signal component. Incubation and washing could be finished automatically on a machine with magnetic rods. Under the optimal conditions, the MB-LIPS showed 96.3% agreement to a commercial enzyme linked immunosorbent assay (ELISA) kit for detecting ASFV antibody in swine sera. Analyzing serial dilutions of a swine serum sample showed that the MP-LIPS assay was 4 times more sensitive than the ELISA kit. The whole run of the MB-LIPS could be completed within 30 min. With its high sensitivity and simple operation, the MB-LIPS platform has great potentials to be used for the detection of ASFV antibody and ASF control in small labs and farms.
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http://dx.doi.org/10.1128/JCM.00990-21DOI Listing
July 2021

High-temperature phonon transport properties of SnSe from machine-learning interatomic potential.

J Phys Condens Matter 2021 Jul 13. Epub 2021 Jul 13.

Shanghai Jiao Tong University, School of Mechanical Engineering, Shanghai, 200240, CHINA.

As a promising thermoelectric material, tin selenide (SnSe) is of relatively low thermal conductivity. However, the phonon transport mechanisms in SnSe are not fully understood due to the complex phase transition, dynamical instability, and strong anharmonicity. In this work, we perform molecular dynamics simulations with a machine-learning interatomic potential to explore the thermal transport properties of SnSe at different temperatures. The developed interatomic potential is parameterized using the framework of moment tensor potential, exhibiting satisfactory predictions on temperature-dependent lattice constants and phonon dispersion, as well as phase transition temperature. From equilibrium molecular dynamics simulations, we obtained the thermal conductivity tensor from 200 K to 900 K. The origins of temperature-dependent thermal conductivity anisotropy and the roles of four-phonon scatterings are identified. The obtained interatomic potential can be utilized to study the mechanical and thermal properties of SnSe and related nanostructures in a wide range of temperatures.
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http://dx.doi.org/10.1088/1361-648X/ac13fdDOI Listing
July 2021

Correlation analysis of long non-coding RNA TUG1 with disease risk, clinical characteristics, treatment response, and survival profiles of adult Ph Acute lymphoblastic leukemia.

J Clin Lab Anal 2021 Jul 12:e23583. Epub 2021 Jul 12.

Department of Hematology, Lanzhou University, Second Hospital, Lanzhou, China.

Background: Long non-coding RNA taurine-upregulated gene 1 (lncRNA TUG1) is reported to be involved in the progression and development of several malignancies; however, its role in Philadelphia chromosome-negative acute lymphoblastic leukemia (Ph ALL) is unknown. The present study aimed to explore the correlation of lncRNA TUG1 with disease risk, disease condition, and prognosis of adult Ph ALL.

Methods: Total 101 adult Ph ALL patients and 40 bone marrow (BM) donors were included, followed by detection of BM monocyte cell lncRNA TUG1 expression by reverse transcription-quantitative polymerase chain reaction. According to the quantiles of lncRNA TUG1 expression in Ph ALL patients, these patients were divided into four tiers: tier 1 (ranked in 0%~25%), tier 2 (ranked in 25%~50%), tier 3 (ranked in 50%~75%), and tier 4 (ranked in 75%~100%).

Results: LncRNA TUG1 was upregulated in Ph ALL patients compared with healthy donors. Further analysis indicated that in Ph ALL patients, higher lncRNA TUG1 tier was correlated with the presence of central nervous system leukemia, increased white blood cell level, and bone marrow blasts. Furthermore, higher lncRNA TUG1 tier was negatively associated with complete remission (CR) within 4 weeks, total CR, and allogeneic hematopoietic stem cell transplant achievement. In addition, higher lncRNA TUG1 tier was associated with decreased disease-free survival and overall survival, which was further verified to be an independent factor by Cox's regression analysis.

Conclusion: lncRNA TUG1 presents potential to be a novel biomarker for disease risk assessment and survival surveillance in Ph ALL management.
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http://dx.doi.org/10.1002/jcla.23583DOI Listing
July 2021

Identification of a Novel Transcription Factor Prognostic Index for Breast Cancer.

Front Oncol 2021 24;11:666505. Epub 2021 Jun 24.

Department of Oncology, Xiangya Hospital, Central South University, Changsa, China.

Transcription factors (TFs) are the mainstay of cancer and have a widely reported influence on the initiation, progression, invasion, metastasis, and therapy resistance of cancer. However, the prognostic values of TFs in breast cancer (BC) remained unknown. In this study, comprehensive bioinformatics analysis was conducted with data from The Cancer Genome Atlas (TCGA) and Gene Expression Omnibus (GEO) database. We constructed the co-expression network of all TFs and linked it to clinicopathological data. Differentially expressed TFs were obtained from BC RNA-seq data in TCGA database. The prognostic TFs used to construct the risk model for progression free interval (PFI) were identified by Cox regression analyses, and the PFI was analyzed by the Kaplan-Meier method. A receiver operating characteristic (ROC) curve and clinical variables stratification analysis were used to detect the accuracy of the prognostic model. Additionally, we performed functional enrichment analysis by analyzing the differential expressed gene between high-risk and low-risk group. A total of nine co-expression modules were identified. The prognostic index based on 4 TFs (NR3C2, ZNF652, EGR3, and ARNT2) indicated that the PFI was significantly shorter in the high-risk group than their low-risk counterpart (p < 0.001). The ROC curve for PFI exhibited acceptable predictive accuracy, with an area under the curve value of 0.705 and 0.730. In the stratification analyses, the risk score index is an independent prognostic variable for PFI. Functional enrichment analyses showed that high-risk group was positively correlated with mTORC1 signaling pathway. In conclusion, the TF-related signature for PFI constructed in this study can independently predict the prognosis of BC patients and provide a deeper understanding of the potential biological mechanism of TFs in BC.
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http://dx.doi.org/10.3389/fonc.2021.666505DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8264286PMC
June 2021

An optimized free induction decay signal sensing coil and its matching circuit for miniaturized Overhauser geomagnetic sensor.

Rev Sci Instrum 2021 May;92(5):055005

Science and Technology on Near-Surface Detection Laboratory, Wuxi 214035, China.

An Overhauser geomagnetic sensor is a precise instrument commonly employed for geomagnetic field observation, magnetic surveys, and so on. Currently, the miniaturization of the Overhauser geomagnetic sensor is limited due to the lower signal-to-noise ratio. Thus, how to effectively extract weaker free induction decay (FID) signal from a miniaturized sensor and how to improve the signal quality have become the bottleneck. To address these problems, we came up with an optimal design of the FID signal sensing coil for a miniaturized Overhauser geomagnetic sensor and propose a front-end matching circuit for the sensing coil to inhibit the attenuation of the signal amplitude caused by high impedance, further reducing the overall noise floor of the signal acquisition system. Finally, the field experimental results show that the miniaturized prototype sensor has a smaller volume and mass with an approximate performance compared with the commercial sensor.
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http://dx.doi.org/10.1063/5.0032431DOI Listing
May 2021

How Much Benefit Can Patients Acquire from Enhanced Recovery After Surgery Protocols with Percutaneous Endoscopic Lumbar Interbody Fusion?

Int J Gen Med 2021 2;14:3125-3132. Epub 2021 Jul 2.

Department of Orthopedics, The Second Affiliated Xinqiao Hospital of Army Military Medical University, Chongqing, People's Republic of China.

Purpose: We aimed to explore the role of enhanced recovery after surgery (ERAS) in patients who underwent percutaneous endoscopic lumbar interbody fusion (PELIF).

Patients And Methods: We performed a retrospective, observational, cohort study on 91 patients who underwent PELIF for degenerative disc disease. The primary outcomes were postoperative opioid consumption, hospital length of stay (LOS), and hospital cost.

Results: Forty-six patients comprised the ERAS group, and 45 patients comprised the pre-ERAS group (control group). The groups had comparable demographic characteristics. Good compliance with the ERAS pathway was observed in the ERAS group. Patients in the ERAS group used significantly fewer morphine equivalents compared with the pre-ERAS group (25.0 vs 33.3, respectively; p = 0.017). Hospital LOS did not decrease significantly in the ERAS group compared with the pre-ERAS group (3.1days vs 3.4 days, respectively; p = 0.096). Likewise, there was no significant difference in hospital cost between the pre-ERAS group and the ERAS group ($10,598.60 vs $10,384.50, respectively; p = 0.468).

Conclusion: In the present study, the benefit of ERAS in the context of PELIF was limited. Although a multidisciplinary ERAS protocol can improve analgesia and decrease opioid consumption, no significant reduction in hospital LOS and cost was observed.
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http://dx.doi.org/10.2147/IJGM.S318876DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8260044PMC
July 2021

Folic Acid Inhibits Aging-Induced Telomere Attrition and Apoptosis in Astrocytes In Vivo and In Vitro.

Cereb Cortex 2021 Jul 5. Epub 2021 Jul 5.

Department of Nutrition and Food Science, School of Public Health, Tianjin Medical University, Tianjin 300070, China.

Folic acid (FA) has been reported to inhibit astrocyte apoptosis and improve aging-induced disorders; however, its role in telomere attrition remains unclear. In present study, 4-month-old senescence-accelerated mouse prone 8 (SAMP8) mice were assigned to four treatment groups for the in vivo experiment: FA-deficient diet (FA-D) group, FA-normal diet (FA-N) group, low FA-supplemented diet (FA-L) group, and high FA-supplemented diet (FA-H) group. These mice were euthanized when 10 months old. There was also a young SAMP8 (4 months old) control group (Con-Y) fed with FA-normal diet. In in vitro study, primary cultures of astrocytes from hippocampus and cerebral cortex were incubated for five generations with various concentrations of FA (0-40 μM) and were assigned to five groups: FA 0 μM (generation 5), FA 10 μM (generation 5), FA 20 μM (generation 5), FA 40 μM (generation 5), and FA 10 μM (generation 1). The results showed that FA supplementation inhibited aging-induced astrocytosis, astrocyte apoptosis, neurodegeneration, and prevented telomere attrition in hippocampus and cortex of SAMP8 mice. FA supplementation also decreased apoptosis and telomere attrition, and increased telomerase activity, in primary cultures of astrocytes. These results showed that it may be one of the mechanisms that FA inhibiting aging-induced apoptosis of astrocyte by alleviating telomere attrition.
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http://dx.doi.org/10.1093/cercor/bhab208DOI Listing
July 2021

Diosmetin Protects Against Obesity and Metabolic Dysfunctions Through Activation of Adipose Estrogen Receptors in Mice.

Mol Nutr Food Res 2021 Jul 5:e2100070. Epub 2021 Jul 5.

Key Laboratory of Animal Genetics, Breeding and Reproduction of Shaanxi Province, College of Animal Science and Technology, Northwest A&F University, Yangling, Shaanxi, China.

Scope: Obesity is a major public health and economic problem of global significance. Here, we investigate the role of diosmetin, a natural flavonoid presents mainly in citrus fruits, in the regulation of obesity and metabolic dysfunctions in mice.

Methods And Results: Eight-week-old male C57BL/6 mice fed a high-fat diet (HFD) or 5-week-old male ob/ob mice fed a normal diet are treated with diosmetin (50 mg kg daily) or vehicle for 8 weeks. Diosmetin treatment decreases body weight and fat mass, improves glucose tolerance and insulin resistance in obese mice. These metabolic benefits are mainly attributed to increase energy expenditure via enhancing thermogenesis in brown adipose tissue (BAT) and browning of white adipose tissue (WAT). Mechanistically, diosmetin acts as an agonist for estrogen receptors (ERs), and subsequently elevates adipose expressions of ERs in mice and in cultured adipocytes. When ERs are blocked by their antagonist fulvestrant in mice, diosmetin loses its beneficial effects, suggesting that ERs are indispensable for the metabolic benefits of diosmetin.

Conclusion: The results indicate that diosmetin may be a potential anti-obesity nutritional supplement and could be explored for low ERs-related obesity populations.
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http://dx.doi.org/10.1002/mnfr.202100070DOI Listing
July 2021

LDHA is enriched in human islet alpha cells and upregulated in type 2 diabetes.

Biochem Biophys Res Commun 2021 Jul 1;568:158-166. Epub 2021 Jul 1.

Centre for Biomolecular Interactions Bremen, University of Bremen, Bremen, Germany; Department of Molecular Medicine, School of Advanced Technologies in Medicine, Tehran University of Medical Sciences, Tehran, Iran. Electronic address:

The lactate dehydrogenase isoform A (LDHA) is a key metabolic enzyme that preferentially catalyzes the conversion of pyruvate to lactate. Whereas LDHA is highly expressed in many tissues, its expression is turned off in the differentiated adult β-cell within the pancreatic islets. The repression of LDHA under normal physiological condition and its inappropriate upregulation under a diabetogenic environment is well-documented in rodent islets/β-cells but little is known about LDHA expression in human islet cells and whether its abundance is altered under diabetic conditions. Analysis of public single-cell RNA-seq (sc-RNA seq) data as well as cell type-specific immunolabeling of human pancreatic islets showed that LDHA was mainly localized in human α-cells while it is expressed at a very low level in β-cells. Furthermore, LDHA, both at mRNA and protein, as well as lactate production is upregulated in human pancreatic islets exposed to chronic high glucose treatment. Microscopic analysis of stressed human islets and autopsy pancreases from individuals with type 2 diabetes (T2D) showed LDHA upregulation mainly in human α-cells. Pharmacological inhibition of LDHA in isolated human islets enhanced insulin secretion under physiological conditions but did not significantly correct the deregulated secretion of insulin or glucagon under diabetic conditions.
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http://dx.doi.org/10.1016/j.bbrc.2021.06.065DOI Listing
July 2021

LRRK2 is a candidate prognostic biomarker for clear cell renal cell carcinoma.

Cancer Cell Int 2021 Jul 3;21(1):343. Epub 2021 Jul 3.

Department of Pathology, Zhongnan Hospital of Wuhan University, Wuhan, China.

Background: Clear cell renal cell carcinoma (ccRCC), derived from renal tubular epithelial cells, is the most common malignant tumor of the kidney. The study of key genes related to the pathogenesis of ccRCC has become important for gene target therapy.

Methods: Bioinformatics analysis of The Cancer Genome Atlas (TCGA), the NCBI Gene Expression Omnibus (GEO) database, USUC Xena database, cBioPortal for Cancer Genomics, and MethSurv were performed to examine the aberrant genetic pattern and prognostic significance of leucine-rich repeat kinase 2 (LRRK2) expression and its relationship to clinical parameters. Immunohistochemistry and Western blot were performed to verify LRRK2 expression. The regulation of ccRCC tumor cell lines proliferation by LRRK2 was examined by CCK8 assay.

Results: Bioinformatics analysis showed that LRRK2 expression was up-regulated and largely correlated with DNA methylation in ccRCC. The up-regulation of LRRK2 was confirmed in ccRCC tissue immunohistochemically and by protein analysis. The level of expression was related to gender, pathological grade, stage, and metastatic status of ccRCC patients. Meanwhile, Kaplan-Meier analysis showed that high expression of LRRK2 correlates to a better prognosis; knockdown of LRRK2 expression attenuated the proliferation ability of ccRCC tumor cell lines; protein-protein interaction network analysis showed that LRRK2 interacts with HIF1A and EGFR.

Conclusion: We found that LRRK2 may play an important role in the tumorigenesis and progression of ccRCC. Our findings provided a potential predictor and therapeutic target in ccRCC.
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http://dx.doi.org/10.1186/s12935-021-02047-yDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8254929PMC
July 2021

DMEP induces mitochondrial damage regulated by inhibiting Nrf2 and SIRT1/PGC-1α signaling pathways in HepG2 cells.

Ecotoxicol Environ Saf 2021 Sep 30;221:112449. Epub 2021 Jun 30.

College of Food Science, South China Agricultural University, Guangzhou 510642, China; Guangdong Provincial Key Laboratory of Food Quality and Safety, Guangzhou 510642, China. Electronic address:

Dimethoxyethyl phthalate (DMEP) is an environmental endocrine disruptor. However, research into the underlying mechanisms of DMEP mitochondrial toxicity is still in its infancy. We therefore expect to understand whether DMEP induced mitochondrial damage in HepG2 cells and the associated signaling pathways. DMEP (0.125, 0.25, 0.5, 1 and 2 mM) exposure for 48 h induced a notable increment in reactive oxygen species (ROS), malondialdehyde (MDA), alanine aminotransferase (ALT), aspartate transaminase (AST) and 8-hydroxydeoxyguanosine (8-OHdG) in hepG2 cells, resulting in cellular oxidative stress. Low doses of DMEP upregulated nuclear factor E2-related factor 2 (Nrf2) and downstream protein haeme oxygenase-1 (HO-1) levels and high doses down-regulated their levels. Nrf2 levels increased after ROS scavenging by N-acetyl-L-cysteine (NAC), which indicated that the Nrf2 pathway may be affected by oxidative stress. We also found that DMEP decreased ATP content, mitochondrial copy number (mtDNA), translocase of the outer membrane subunit 20 (TOM20) expression, mitochondria-encoded genes CO1, CO2, CO3, ATP6, ATP8 expression, inhibited mitochondrial biogenesis pathway, down-regulated sirtuin 1(SIRT1), PPAR gamma co-activator 1 alpha (PGC-1α), Nuclear respiratory factor 1(Nrf1), Mitochondrial transcription factor A (TFAM) content and activated PINK1/Parkin autophagy pathway. DMEP also activated the mitochondrial apoptotic pathway, causing cytochrome c cytoplasmic translocation and caspase 3 cleavage. What's more, DMEP activated the Nuclear factor-κB (NF-κB) pathway and levels of tumor necrosis factor-α (TNF-α), interleukin-1β (IL-1β) and interleukin-6 (IL-6) were significantly upregulated, causing an inflammatory response. In summary, DMEP can cause inflammatory response and oxidative stress in HepG2 cells, inhibited the Nrf2 pathway and mitochondrial biogenesis, and induced autophagy and apoptosis. And oxidative stress at least partially affected the Nrf2 pathway and mitochondrial biogenesis SIRT1/PGC-1α pathway.
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http://dx.doi.org/10.1016/j.ecoenv.2021.112449DOI Listing
September 2021

Establishment of a Multiplex RT-PCR Method for the Detection of Five Known Genotypes of Porcine Astroviruses.

Front Vet Sci 2021 15;8:684279. Epub 2021 Jun 15.

College of Animal Science and Technology, Guangxi University, Nanning, China.

Porcine astroviruses (PAstVs) are prevalent in pigs worldwide, and five genotypes have been reported to circulate in China. However, little is known about the coinfection status of PAstVs. For differential and simultaneous diagnoses of these five genotypes of PAstVs, a multiplex RT-PCR method was established on the basis of the gene of type 1 PAstV, and the genes of type two to five PAstVs. This quintuple PCR system was developed through optimization of multiplex PCR and detection sensitivity and specificity. The results showed that this multiplex RT-PCR method could specifically detect all the five PAstV genotypes without cross-reaction to any other major viruses circulating in Chinese pig farms. The detection limit of this method was as low as 10 pg of standard plasmids of each PAstV genotype. In addition, a total of 275 fecal samples collected from different districts of Guangxi, China, between April 2019 and November 2020, were tested by this newly established multiplex RT-PCR. Moreover, the sensitivity and specificity of monoplex and multiplex RT-PCR methods were compared by detecting the same set of clinical positive samples. The results revealed that PAstV1 (31/275), PAstV2 (49/275), PAstV3 (36/275), PAstV4 (41/275), and PAstV5 (22/275) were all detected, and dual (PAstV1+PAstV2, PAstV1+PAstV3, PAstV2+PAstV3, PAstV2+PAstV4, PAstV3+PAstV4, and PAstV4+PAstV5) or triple genotypes (PAstV1+PAstV2+PAstV3 and PAstV2+PAstV3+PAstV4) of coinfections were also unveiled in this study. The detection result of multiplex PCR was consistent with that of monoplex PCR. Compared with monoplex PCR, this multiplex PCR method showed obvious advantages such as time and cost efficiency and high sensitivity and specificity. This multiplex RT-PCR method offered a valuable tool for the rapid and accurate detection of PAstV genotypes circulating in pig herds and will facilitate the surveillance of PAstV coinfection status.
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http://dx.doi.org/10.3389/fvets.2021.684279DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8239161PMC
June 2021

Mitochondrial creatine kinase 1 in non-small cell lung cancer progression and hypoxia adaptation.

Respir Res 2021 Jul 1;22(1):190. Epub 2021 Jul 1.

Department of Internal Medicine Ward IV, Shandong Provincial Chest Hospital, 12# Lieshishandong Road, Jinan, Shandong, China.

Background: Hypoxia is a prominent feature of solid cancer. This research aims to expose the role of mitochondrial creatine kinase 1 (CKMT1) in non-small cell lung cancer (NSCLC) progression and hypoxia adaptation.

Methods: The mRNA and protein expression of CKMT1 in NSCLC tissues were detected by using GEPIA web, immunohistochemistry and qRT-PCR. For hypoxia, cells were exposed to the 1% O atmosphere. The protein levels of HIF-1α and CKMT1 in H1650 and H1299 cells exposed to hypoxia were determined by western blot. The roles of CKMT1 on the proliferation, invasion and hypoxia adaptation of NSCLC cells were measured by CCK8, colony formation and transwell assays. Luciferase activity assay and HIF1 specific inhibitor (LW6) assay indicated the related function of hypoxia and CKMT1.

Results: CKMT1 was highly expressed in NSCLC tissues, and the high level of CKMT1 was significantly correlated with the high pathological grade of NSCLC. Knockdown of CKMT1 inhibited the cell proliferation and invasion of H1650 and H1299 cells, which could be rescued by hypoxia. Hypoxia induced the accumulation of HIF-1α and the expression of CKMT1 in H1650 and H1299 cells. Furthermore, HIF-1 as a transcription factor of CKMT1, could up-regulated the expression of CKMT1 under hypoxia.

Conclusions: In summary, CKMT1 has the potential as a target for NSCLC hypoxic targeted therapy.
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http://dx.doi.org/10.1186/s12931-021-01765-1DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8247087PMC
July 2021

Comprehensive Evaluation of Volatile and Nonvolatile Compounds in Oyster Cuts of Roasted Lamb at Different Processing Stages Using Traditional Nang Roasting.

Foods 2021 Jun 29;10(7). Epub 2021 Jun 29.

College of Food Science and Technology, Hebei Agricultural University, Baoding 071000, China.

Nang roasting is a traditional lamb processing method in Xinjiang (China) with a history of thousands of years. This study comprehensively evaluated the volatile and nonvolatile compounds of oyster cuts of roasted lamb at different processing stages of Nang roasting using gas chromatography mass spectrometry and amino acid automatic analyzer, respectively. Results indicated that aldehydes were the dominant profiles of volatile compounds, and hexanal, nonanal, octanal, (E)-2-nonenal, (E, E)-2,4-decadienal, (E, E)-2,4-nonadienal and 1-octen-3-ol were the key volatile compounds or aroma contributors to roasted oyster cuts. Isoamylol and 3-hydroxy-2-butanone could differentiate fresh and marinated oyster cuts from roasted ones; (E)-2-nonenal, (E, E)-2,4-decadienal, 1-octen-3-ol, hexanal, octanal, nonanal and (E, E)-2,4-nonadienal could differentiate Nang roasted oyster cuts of 60 min from those of 15, 30 and 45 min. Umami amino acids and sweet amino acids are the dominant profiles of nonvolatile compounds; glutamic acid, alanine and 5'-IMP were the key free amino acids or taste contributors to roasted oyster cuts. Glutamic acid, alanine and 5'-IMP could differentiate fresh and marinated oyster cuts from roasted samples. This work provided theoretical support for the control of flavor attributes of roasted lamb with traditional Nang roasting.
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http://dx.doi.org/10.3390/foods10071508DOI Listing
June 2021

Effects of Phthalate Esters (PAEs) on Cell Viability and Nrf2 of HepG2 and 3D-QSAR Studies.

Toxics 2021 Jun 5;9(6). Epub 2021 Jun 5.

College of Food Science, South China Agricultural University, Guangzhou 510642, China.

Phthalate esters (PAEs) are a widespread environmental pollutant, and their ecological and environmental health risks have gradually attracted attention. To reveal the toxicity characteristics of these compounds, ten PAEs were selected as research objects to establish a cell model. CCK-8 was used to determine cell viability, Western blots were used to determine the content of Nrf in HepG2, and the LD collected for the 13 PAEs administered to rats. On this basis, 3D-QSAR models of IC, LD and Nrf2 were established. The experimental results showed that as the time of PAEs exposure increased (24, 48 and 72 h), cell viability gradually decreased. The test concentration (62.5 /125/250 μM) of PAEs exposed for 48 h could significantly increase the content of Nrf2, and the 1000 μM PAEs could inhibit the content of Nrf2. The model is relatively stable and predicts well that the introduction of large and hydrophobic groups may significantly affect the toxic effects of PAEs on cells. The present study provided a potential tool for predicting the LD50 and Nrf2 of new PAEs, and provide a reference for the design of new less toxic PAEs in the future.
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http://dx.doi.org/10.3390/toxics9060134DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8228614PMC
June 2021

Antithrombotic effects of Huanglian Jiedu decoction in a rat model of ischaemia-reperfusion-induced cerebral stroke.

Pharm Biol 2021 Dec;59(1):823-827

Jiangxi University of Technology, Nanchang, Jiangxi, China.

Context: Huanglian Jiedu Decoction (HLJJD) has a variety of pharmacological activities, such as anti-inflammatory and neuroprotection against ischaemic brain injury.

Objectives: This study explores its antithrombosis activity and inhibition of platelet aggregation.

Material And Methods: To study the antithrombosis activity of HLJJD , saline, or HLJDD (100, 200, and 500 mg/kg) was treated prophylactically by gavage for 3 days in Wistar rats ( = 4). Based on the rat model of transient middle cerebral artery infarction (MCAO) or normal rats ( = 4), the antithrombotic activity in the normal group and HLJDD subgroups on prothrombin time, thrombus weight, platelet aggregation, and others was evaluated, followed by the antiplatelet aggregation of its main components ( = 4).

Results: The weight of the thrombus increased significantly at 24 h after MCAO onset. HLJJD did not influence the change of PT, but significantly inhibited thrombosis by 12.5, 20.0, and 20.5% in reducing the dry weight of thrombus, and blocked collagen-induced platelet aggregation by 25.5, 39.0, and 42.7% and adhesion of blood platelet by 17.3, 26.2, and 27.3%. The IC value of HLJJD on collagen-induced platelet aggregation was 670 mg/kg. Geniposide only facilitated antiplatelet aggregation induced by collagen, but not AA or ADP. Both baicalin and berberine showed markedly antiplatelet aggregation induced by all activators. The antithrombotic activity of baicalin was relatively higher than that of berberine (35.0-47.8% 20.6-33.5%).

Conclusion: Our results indicated that HLJDD regulated blood circulation by inhibiting platelet aggregation and thrombosis, which might also extensively contribute to the clinical prevention and treatment of cerebrovascular diseases.
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http://dx.doi.org/10.1080/13880209.2021.1942505DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8253176PMC
December 2021

Two MicroRNAs, miR-34a and miR-125a, Are Implicated in Bicuspid Aortopathy by Modulating Metalloproteinase 2.

Biochem Genet 2021 Jun 30. Epub 2021 Jun 30.

Department of Cardiac Surgery, Zhongshan Hospital, Fudan University, Shanghai, 200032, China.

It has been recognized that wall shear stress plays an important role in the development of Bicuspid Aortopathy (BA), but the intrinsic mechanism is not well elucidated. This study aims to explore the underlying relationship between hemodynamical forces and pathological phenomenon. Total RNA was prepared from aortic wall tissues collected from 20 BA patients. RNA sequencing, bioinformatic analysis and quantitative reverse-transcription PCR validation identified nine miRNAs that were up-regulated in the aortic part exposed to high wall shear stress compared to the low wall shear stress control, and six miRNAs that were down-regulated. Among these candidates, miR-34a and miR-125a, both down-regulated in the high wall shear stress parts, were shown to be potential inhibitors of the metalloproteinase 2 gene. Luciferase reporter assays confirmed that both miRNAs could inhibit the expression of metalloproteinase 2 mRNA in CRL1999 by complementing with its 3' untranslated region. Conversely, immunofluorescence assays showed that inhibition of miR-34a or miR-125a could lead to increased metalloproteinase 2 protein level. On the other hand, both miR-34a and miR-125a were shown to alleviate stretch-induced stimulation of metalloproteinase 2 expression in CRL1999 cells. The results suggested that miR-34a and miR-125a might be implicated in wall shear stress induced aortic pathogenesis due to their apparent regulatory roles in metalloproteinase 2 expression and extracellular matrix remodeling, which are key events in the weakening of aortic walls among BA patients.
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http://dx.doi.org/10.1007/s10528-021-10085-4DOI Listing
June 2021

Pressure-Controlled Volume-Guaranteed Ventilation Improves Respiratory Dynamics in Pediatric Patients During Laparoscopic Surgery: A Prospective Randomized Controlled Trial.

Int J Gen Med 2021 22;14:2721-2728. Epub 2021 Jun 22.

Department of Anesthesiology, The First Affiliated Hospital of Anhui Medical University, Hefei, People's Republic of China.

Purpose: Pressure-controlled volume-guaranteed (PCV-VG) combines the characteristics of pressure-controlled ventilation (PCV) and volume-controlled ventilation (VCV). It has been reported that PCV-VG decreases airway pressure and improves oxygenation among the adult group. In this study, the respiratory dynamics of PCV-VG and VCV are compared in pediatric patients ventilated with laryngeal mask airway and underwent laparoscopic hernia of the sac ligation.

Patients And Methods: Sixty-four pediatric patients were included in this prospective, randomized clinical trial. Pediatric patients were randomly allocated to receive VCV and PCV-VG ventilation during the general anesthesia. The hemodynamic and respiratory variables were recorded at the time when laryngeal mask airway was placed, pneumoperitoneum began, 5 mins after pneumoperitoneum began, pneumoperitoneum ended, and the operation ended respectively. The respiratory adverse events were recorded after the operation and on the first day after the operation. In this study, respiratory adverse events are defined as cough, hoarseness, hypoxemia, laryngospasm, bronchospasm, and sore throat.

Results: There was no statistical difference in hemodynamic variables at all time points between the two groups. Compared to the VCV group, peak airway pressure (Ppeak) and plateau airway pressure in the PCV-VG group decreased significantly. Pulmonary dynamic compliance (Cydn) in the PCV-VG group was significantly higher than that in the VCV group. The respiratory adverse events appeared to have no statistical difference between VCV and PCV groups.

Conclusion: PCV-VG provides a lower Ppeak and better Cydn in pediatric patients compared with the VCV group during laparoscopic surgery. The results suggested that PCV-VG may be a superior way of mechanical ventilation for pediatric patients who ventilated with laryngeal mask airway and experienced laparoscopic surgery.
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http://dx.doi.org/10.2147/IJGM.S318008DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8235931PMC
June 2021

Molecular characterization of atmospheric particulate organosulfates in a port environment using ultrahigh resolution mass spectrometry: Identification of traffic emissions.

J Hazard Mater 2021 Jun 18;419:126431. Epub 2021 Jun 18.

State Key Joint Laboratory of ESPC, State Environmental Protection Key Laboratory of Sources and Control of Air Pollution Complex, School of the Environment, Tsinghua University, Beijing 100084, China. Electronic address:

Organosulfates (OSs) are an important component of atmospheric organic aerosol (OA) and are widespread in various environments. However, the OSs generated from anthropogenic emissions are poorly understood. In this study, the molecular compositions of OSs from atmospheric PM samples collected during a winter measurement campaign (SEISO-Bohai) at Jingtang Harbor were characterized via ultrahigh resolution mass spectrometry (UHRMS). The changes of port OS compositions were observed in episodes of complete haze pollution. As the pollution aggravated, the relative abundances of OSs were apparently increased, and the molecule compositions became more complex, primarily driven by the oxidation and fragmentation processes. Potential OS precursors from traffic emissions were identified based on an optimized "OS precursor map" developed in the previous study. OSs characterized by high molecular weights and low degrees of both unsaturation and oxidization were suggested to mainly derive from secondary reactions of intermediate volatile organic compounds (IVOCs) emitted by traffic sources. These OSs were primarily detected in clean-day samples, followed by decreasing with the pollution process. In addition, our study also finds that ship emissions may further facilitated OS productions under haze pollution conditions.
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http://dx.doi.org/10.1016/j.jhazmat.2021.126431DOI Listing
June 2021

SARS-CoV-2 Host Receptor ACE2 Protein Expression Atlas in Human Gastrointestinal Tract.

Front Cell Dev Biol 2021 11;9:659809. Epub 2021 Jun 11.

Department of Colorectal Surgery, The First Affiliated Hospital, School of Medicine, Zhejiang University, Hangzhou, China.

Background: Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infects host cells through interactions with its receptor, Angiotensin-converting enzyme 2 (ACE2), causing severe acute respiratory syndrome and death in a considerable proportion of people. Patients infected with SARS-CoV-2 experience digestive symptoms. However, the precise protein expression atlas of ACE2 in the gastrointestinal tract remains unclear. In this study, we aimed to explore the ACE2 protein expression pattern and the underlying function of ACE2 in the gastrointestinal tract, including the colon, stomach, liver, and pancreas.

Methods: We measured the protein expression of ACE2 in the gastrointestinal tract using immunohistochemical (IHC) staining with an ACE2-specific antibody of paraffin-embedded colon, stomach, liver, and pancreatic tissues. The correlation between the protein expression of ACE2 and the prognosis of patients with gastrointestinal cancers was analyzed by the log-rank (Mantel-Cox) test. The influence of ACE2 on colon, stomach, liver, and pancreatic tumor cell line proliferation was tested using a Cell Counting Kit 8 (CCK-8) assay.

Results: ACE2 presented heterogeneous expression patterns in the gastrointestinal tract, and it showed a punctate distribution in hepatic cells. Compared to that in parallel adjacent non-tumor tissues, the protein expression of ACE2 was significantly increased in colon cancer, stomach cancer, and pancreatic cancer tissues but dramatically decreased in liver cancer tissues. However, the expression level of the ACE2 protein was not correlated with the survival of patients with gastrointestinal cancers. Consistently, ACE2 did not affect the proliferation of gastrointestinal cancer cells .

Conclusion: The ACE2 protein is widely expressed in the gastrointestinal tract, and its expression is significantly altered in gastrointestinal tumor tissues. ACE2 is not an independent prognostic marker of gastrointestinal cancers.
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http://dx.doi.org/10.3389/fcell.2021.659809DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8226145PMC
June 2021

Increased microRNA-30a levels in bronchoalveolar lavage fluid as a diagnostic biomarker for lung cancer.

PeerJ 2021 9;9:e11528. Epub 2021 Jun 9.

Department of Respiratory and Critical Care Medicine, West China Hospital, Sichuan University, Chengdu, China.

Background: MicroRNA-30a (miRNA-30a) levels have been shown to increase in the plasma of lung cancer patients. Herein, we evaluated the miRNA-30a levels in the bronchoalveolar lavage fluid (BALF) of lung cancer patients as a potential biomarker for lung cancer diagnosis.

Methods: BALF miRNA-30a expression of 174 subjects was quantified using quantitative real-time reverse transcription-polymerase chain reaction and compared between lung cancer patients and control patients with benign lung diseases. Moreover, its diagnostic value was evaluated by performing receiver operating characteristic (ROC) curve analysis.

Results: The relative BALF miRNA-30a expression was significantly higher in the lung cancer patients than in the controls (0.74 ±  0.55 versus 0.07 ±  0.48, respectively,  < 0.001) as well as in lung cancer patients with stage I-IIA disease than in those with stage IIB-IV disease (0.98 ±  0.64 versus 0.66 ±  0.54, respectively,  < 0.05). Additionally, miRNA-30a distinguished benign lung diseases from lung cancers, with an area under the ROC curve (AUC) of 0.822. ROC analysis also revealed an AUC of 0.875 for the Youden index-based optimal cut-off points for stage I-IIA adenocarcinoma. Thus, increased miRNA-30a levels in BALF may be a useful biomarker for non-small-cell lung cancer diagnosis.
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http://dx.doi.org/10.7717/peerj.11528DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8197034PMC
June 2021

Application of Mobile Stroke Unit in Prehospital Thrombolysis of Acute Stroke: Experience from China.

Cerebrovasc Dis 2021 Jun 25:1-6. Epub 2021 Jun 25.

Department of Interventional Neuroradiology, Henan Provincial People's Hospital, Zhengzhou University People's Hospital, Zhengzhou, China.

Background And Purpose: Most patients cannot receive intravenous thrombolytic therapy in the early stage of stroke onset, and the application of mobile stroke unit (MSU) in prehospital intravenous thrombolytic therapy of acute stroke may change this situation. The first MSU in China was put into use in 2017. Herein, we aimed to explore the preliminary experience of MSU in prehospital thrombolysis of acute stroke.

Methods: Patients who received prehospital intravenous thrombolytic therapy using MSU were classified to the MSU thrombolysis group, and the control group consisted of stroke patients admitted by regular ambulances, who were transferred to hospital for intravenous thrombolytic therapy. The feasibility, safety, and duration of procedures were compared.

Results: There were 14 patients received prehospital intravenous thrombolysis on the MSU, and 24 patients underwent intravenous thrombolysis in the emergency center, who were transferred by the ordinary ambulance during the same period. The median call-to-needle time was 59.5 min in the MSU thrombolysis group, while it was 89 min in the control group; the difference between the 2 groups was statistically significant (p = 0.001). The median time from onset to thrombolysis was 70 and 102.5 min, respectively, in the 2 groups (p = 0.002). The percentages of good clinical outcome (modified Rankin Scale score ≤ 2) at 90-day follow-up were 79 and 67%, respectively (p = 0.488). The rate of symptomatic intracranial hemorrhage and mortality during the perioperative period did not differ significantly between 2 groups.

Conclusion: Despite the small sample size, our preliminary experience of the application of MSU in the prehospital thrombosis therapy seems to indicate a significant reduction in time from call to needle, the efficacy of MSU in the treatment of acute stroke needs further experiment and larger sample size to confirm.
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http://dx.doi.org/10.1159/000514370DOI Listing
June 2021

How does air quality affect residents' life satisfaction? Evidence based on multiperiod follow-up survey data of 122 cities in China.

Environ Sci Pollut Res Int 2021 Jun 24. Epub 2021 Jun 24.

School of Political Science and Public Administration, Wuhan University, Wuhan, China.

Since entering the new era, China's socialist contradiction has been transformed into the contradiction between the people's growing need for a better life and the unbalanced and inadequate development. How to improve the quality of people's life through the improvement of air quality has become an important content restricting social development and a key problem to be solved. Based on the life satisfaction (LS) method, this study takes air quality into the individual utility function, and through matching China Health and Retirement Longitudinal Study (CHARLS), two phases of microindividual tracking data with 122 urban environmental quality data innovatively investigate the impact of air quality on residents' LS and its income substitution effect. The results show that air quality significantly reduces residents' LS, among which, different air pollutants and comprehensive air quality AQI have significant negative effects. And PM10 has the highest marginal effect on different LS evaluation, SO2 has the smallest marginal effect, and AQI marginal effect is close to PM10. In terms of group heterogeneity, NO2 and SO2 have group influence differences in age group, regional economic group, gender group, and family per capita income group. But PM10 and AQI do not show group influence heterogeneity, and air quality has significant negative effect on LS of different groups. In addition, the interaction between air quality and income level shows that air quality strengthens the difference of residents' LS caused by income level difference. According to the equilibrium condition of residents' individual utility function, the improvement of air quality by 1% is equivalent to the improvement of residents' LS by 23.4402% of income. Firstly, air quality has an important impact on residents' LS, and different air pollutants have different effects. Secondly, the impact of air quality on LS of different groups is heterogeneous and mainly diversified in age group, regional economic group, gender group, and family per capital income group. Finally, there is substitution effect between air quality and regional GDP growth and household income, which affects residents' LS. Thirdly, the conclusion shows that the improvement of air quality is difficult to be replaced by other ways. Good air quality can not only directly improve residents' LS, but also has economic effect.
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http://dx.doi.org/10.1007/s11356-021-15022-xDOI Listing
June 2021