Publications by authors named "Huan Liu"

1,864 Publications

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Mask-wearing behavior during COVID-19 in China and its correlation with e-health literacy.

Front Public Health 2022 22;10:930653. Epub 2022 Jul 22.

Department of Health Management Center, The First Affiliated Hospital of Wannan Medical College, Wuhu, China.

Background: During the Coronavirus COVID-19) pandemic, wearing masks became crucial for preventing infection risk and maintaining basic health. Therefore, it is necessary to understand the behavioral characteristics of the mask-wearing public to provide theoretical reference for the prevention and control of COVID-19.

Methods: We conducted a nationwide survey on the mask-wearing behavior of the public and their health literacy levels by distributing electronic questionnaires. Univariate and logistic regression analyses were performed to determine the factors influencing mask-wearing behavior. Pearson's correlation was used to analyze the correlation between mask-wearing behaviors and e-health literacy.

Results: A total of 1,972 valid questionnaires were collected; 99.8% of the public wore masks when going out and 59.2% showed good mask-wearing behavior. Most people choose to wear disposable medical masks (61.3%), followed by medical surgical masks (52.9%). All participants indicated that they had understood the information on how to use masks, and most obtained it through social media (61.8%). The average of the e-health literacy scores of those with good mask-wearing behavior was significantly higher than those with poor mask-wearing behavior ( < 0.05), and each item score of the former's e-health literacy was significantly higher than the latter ( < 0.05). Further, there was a positive correlation between mask-wearing behavior and e-health literacy ( = 0.550, < 0.05). Logistic regression analysis showed that seven factors are related to mask-wearing behavior, including gender, place of residence, educational level, work or living environment, marital status, flu symptoms, and whether living with people in home quarantine ( < 0.01).

Conclusion: The overall compliance of the public's mask-wearing behavior in China during COVID-19 is good. However, there are shortcomings regarding the selection, use, and precautions. The differences in mask-wearing behavior are related to factors including gender, place of residence, educational level, work or living environment, marital status, presence of flu symptoms, and whether living with people in home quarantine. Higher levels of e-health literacy indicated better mask-wearing behavior. Therefore, it is necessary to strengthen the public's popularization and education regarding the prevention and control of COVID-19.
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http://dx.doi.org/10.3389/fpubh.2022.930653DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9354616PMC
August 2022

Recent advances in PLGA micro/nanoparticle delivery systems as novel therapeutic approach for drug-resistant .

Front Bioeng Biotechnol 2022 22;10:941077. Epub 2022 Jul 22.

Department of Respiratory, Shenyang Tenth People's Hospital, Shenyang Chest Hospital, Shenyang, China.

is a severe infectious disease caused by and is a significant public health concern globally. The World Health Organization (WHO) recommends a combination regimen of several drugs, such as rifampicin (RIF), isoniazid (INH), pyrazinamide (PZA), and ethambutol (ETB), to treat t. However, these drugs have low plasma concentrations after oral administration and require multiple high doses, which may lead to the occurrence and development of drug-resistant . Micro/Nanotechnology drug delivery systems have considerable potential in treating drug-resistant , allowing the sustained release of the drug and delivery of the drug to a specific target. These system properties could improve drug bioavailability, reduce the dose and frequency of administration, and solve the problem of non-adherence to the prescribed therapy. This study systematically reviewed the recent advances in PLGA micro/nanoparticle delivery systems as a novel therapeutic approach for drug-resistant .
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http://dx.doi.org/10.3389/fbioe.2022.941077DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9355142PMC
July 2022

The MVBML approach: a management for buccal cancer with wider indications and higher satisfaction.

Oral Dis 2022 Aug 4. Epub 2022 Aug 4.

Department of Oral and Maxillofacial-Head and Neck Oncology, Beijing Stomatological Hospital, Capital Medical University, 100050, Beijing, P.R. China.

Objective: The purpose of this study is to explore the effects of MVBML approach on postoperative facial scar, nerves injury and prognosis of patients with BSCC.

Patients And Methods: This is a single-center, prospective cohort study that enrolled 80 patients with BSCC from June 2015 to December 2020. According to the different surgical approaches, the patients were divided into two groups: the lower lip median (LLM) approach group and the modified vermilion border-marionette line (MVBML) approach group.

Results: The results showed that the appearance (p=0.003), scar consciousness (p<0.001) and satisfaction with appearance (p=0.001) of patients in the MVBML group were significantly better than those in the LLM group, and the difference was more obvious in elderly group. Statistical analysis of postoperative nerves injury showed that the MVBML group had a lower risk of facial and mental nerves injury than the LLM group, and there was a significant statistical difference in mental nerve injury between the two groups (p<0.001). Through Kaplan-Meier survival analysis, we found no significant difference in disease-specific survival (p=0.47) or disease-free survival (p=0.70) between the LLM approach group and the MVBML approach group.

Conclusions: The MVBML surgical approach is worthy of advancement for the surgical treatment of BSCC.
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http://dx.doi.org/10.1111/odi.14340DOI Listing
August 2022

Screening of cell-virus, cell-cell, gene-gene crosstalk among animal kingdom at single cell resolution.

Clin Transl Med 2022 Aug;12(8):e886

China National GeneBank, Shenzhen, China.

Background: The exact animal origin of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) remains obscure and understanding its host range is vital for preventing interspecies transmission.

Methods: Herein, we applied single-cell sequencing to multiple tissues of 20 species (30 data sets) and integrated them with public resources (45 data sets covering 26 species) to expand the virus receptor distribution investigation. While the binding affinity between virus and receptor is essential for viral infectivity, understanding the receptor distribution could predict the permissive organs and tissues when infection occurs.

Results: Based on the transcriptomic data, the expression profiles of receptor or associated entry factors for viruses capable of causing respiratory, blood, and brain diseases were described in detail. Conserved cellular connectomes and regulomes were also identified, revealing fundamental cell-cell and gene-gene cross-talks from reptiles to humans.

Conclusions: Overall, our study provides a resource of the single-cell atlas of the animal kingdom which could help to identify the potential host range and tissue tropism of viruses and reveal the host-virus co-evolution.
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http://dx.doi.org/10.1002/ctm2.886DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9345398PMC
August 2022

Identification of Key Headspace Volatile Compounds Signaling Preference for Rice over Corn in Adult Females of the Rice Leaf Folder .

J Agric Food Chem 2022 Aug 2. Epub 2022 Aug 2.

State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100193, China.

Volatile organic compounds are important for herbivorous insects in locating their host plants. The rice leaf folder, (Guenée), is a devastating migratory insect pest of rice in Asian countries. Although can develop even better on corn than on rice plants in insectaries, it rarely occurs on corn plants in the field. We hypothesized that plant volatile-mediated oviposition preference for rice over corn in adult females may be the reason for the observed rare field occurrence of the pest on corn plants. The present study was conducted to identify the olfactory active volatile compounds (OAVCs) that enable females to discriminate rice from corn plants. In cage tests, rice plants were highly preferred for oviposition over corn plants by females. From headspace, chemical analyses identified 15 rice unique, 8 corn unique, and 28 common volatile compounds. Fourteen OAVCs, including seven common, five rice unique, and two corn unique, were determined. In electroantennogram tests, the rice unique and common OAVCs activated the antennal responses in . In Y-tube olfactometer tests, ()-2-hexenal and 3-hexanol(common OAVCs) and ()-3-hexenyl acetate and ()-2-hexen-1-ol (rice unique OAVCs) attracted more females than the control, and only blends with both rice unique and common OAVCs were highly preferred over the control. Our results provide insights into the chemical cues used by adult females in host location, which may aid the development of novel crop protection strategies based on the manipulation of host-finding behaviors of .
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http://dx.doi.org/10.1021/acs.jafc.2c01948DOI Listing
August 2022

Immobilizing diamine oxidase on electroactive phase-change microcapsules to construct thermoregulatory smart biosensor for enhancing detection of histamine in foods.

Food Chem 2022 Jul 22;397:133759. Epub 2022 Jul 22.

State Key Laboratory of Organic-Inorganic Composites, Beijing University of Chemical Technology, Beijing 100029, China. Electronic address:

Aiming at enhancing the biosensing detection of histamine in foods at high temperature, we developed a thermoregulatory biosensor based on diamine oxidase-immobilized phase-change microcapsules consisting of an n-docosane core, a TiO shell, and an electroactive polyaniline/ZnO composite coating layer. The microcapsules exhibit a satisfactory latent heat capacity of over 112 J/g for thermo-temperature regulation. Through an innovative integration of electroactive phase-change microcapsules and biological enzyme in the working electrode, the biosensor obtained a thermoregulatory function through reversible phase transitions by the n-docosane core under high-temperature environments. This enables the biosensor to achieve a higher response sensitivity of 28.57 µA⋅mM⋅cm and a lower detection limit of 0.473 µmol/L at the high assay temperatures compared to conventional histamine biosensors. With enhanced electrochemical biosensing performance through in-situ thermo-temperature regulation, the smart biosensor developed in this study has found practical applications for high-sensitive detection and high-accurate quantitive determination of histamine in foods across a broad temperature range.
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http://dx.doi.org/10.1016/j.foodchem.2022.133759DOI Listing
July 2022

[One-step rapid enrichment and detection of malachite green in aquaculture water based on metal-organic framework hydrogel].

Se Pu 2022 Aug;40(8):721-729

Key Laboratory of Control of Quality and Safety for Aquatic Products, Ministry of Agriculture and Rural Affairs, Chinese Academy of Fishery Sciences, Beijing 100141, China.

Malachite green is a triphenylmethane compound, which has a good effect on disease prevention and control in the breeding of aquatic products, but it is a prohibited drug because it is detrimental to human health. Owing to the low content of target malachite green and complex components in the actual sample, simultaneously achieving good enrichment, high sensitivity, convenience, and rapidity detecting is difficult. Metal-organic framework (MOF) has a multidimensional network structure, good stability, and large specific surface area, and has broad application prospects in adsorption. However, the small particle size of MOF materials and the difficulty of recycling hinder their development. The hydrogel has a three-dimensional network structure that can encapsulate the MOF nanomaterials in the network, enhancing adsorption performance and facilitating separation from the adsorbed solution. In this study, MOF materials were prepared, and hydrogel doped with MOF nanomaterials (polyacrylamide-sodium alginate/metal-organic framework, PAAM-SA/MOF) was used to investigate the adsorption of malachite green in aquaculture water. The transmission electron microscopy was used to characterize MOF nanomaterials, scanning electron microscopy was used to examine the morphology and structure of the hydrogels before and after adsorption of malachite green, representing the successful synthesis of adsorbent materials with excellent properties. The mechanical properties of the hydrogels were investigated using a tensile testing machine, with a maximum tensile strain of up to 300%, without breaking and failing to remove when separated from the solution to be tested. The pore size of PAAM-SA/MOF hydrogel is considerably smaller than that of PAAM-SA, which is beneficial to increase the specific surface area of the adsorbent and thus improve the adsorption performance. A series of optimizations were performed on the adsorption conditions of the hydrogel adsorbent and the optimized conditions were obtained as follows: the amount of adsorbent used was 0.1 g, adsorption time was 5 h, the pH of malachite green solution was 9, the adsorption temperature was 40 ℃, and the initial concentration of malachite green solution was 100 mg/L. Under these conditions the adsorption efficiency could reach up to 97%. Furthermore, the adsorbed malachite green was eluted with organic solvents of various polarities, and the highest desorption efficiency was achieved when acetonitrile with higher polarity was used as the eluent. Simultaneously, the eluent volume was optimized, with 2 mL acetonitrile added to the malachite green-enriched hydrogel adsorbent to achieve the highest desorption efficiency of 99%. The enriched sample was separated using a Dionex Bonded Silica Products C column (50 mm×2.1 mm, 3 μm), and eluted with an ammonium acetate-acetonitrile solvent system. The results showed that the limit of detection (LOD, =3) was 0.083 μg/L, the limit of quantification (LOQ, =10) was 0.25 μg/L, and the spiked recoveries of malachite green at high, medium, and low levels were 84.8%-118.1% with the relative standard deviations less than 5.1%. The pretreatment is simplified using this approach and combines the respective advantages of MOF and hydrogel to enable one-step enrichment of malachite green in aquaculture water. The additional MOF material can exert good adsorption in the hydrogel system, which solves the problem of low recovery of traditional MOF materials caused by tiny particle size, facilitating direct extraction after adsorption, and also solves the problem of low adsorption efficiency of pure hydrogel, improving the overall adsorption efficiency and recyclability. The actual sample test shows that the new hydrogel adsorption material can be used to extract and detect trace malachite green in aquaculture water. It is a novel, fast and convenient pretreatment approach with great potential in food detection.
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http://dx.doi.org/10.3724/SP.J.1123.2022.04019DOI Listing
August 2022

Transformer Feature Enhancement Network with Template Update for Object Tracking.

Sensors (Basel) 2022 Jul 12;22(14). Epub 2022 Jul 12.

School of Computer Science and Engineering, Xi'an Technological University, Xi'an 710021, China.

This paper proposes a tracking method combining feature enhancement and template update, aiming to solve the problems of existing trackers lacking global information attention, weak feature characterization ability, and not being well adapted to the changing appearance of the target. Pre-extracted features are enhanced in context and on channels through a feature enhancement network consisting of channel attention and transformer architectures. The enhanced feature information is input into classification and regression networks to achieve the final target state estimation. At the same time, the template update strategy is introduced to update the sample template judiciously. Experimental results show that the proposed tracking method exhibits good tracking performance on the OTB100, LaSOT, and GOT-10k benchmark datasets.
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http://dx.doi.org/10.3390/s22145219DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9320290PMC
July 2022

[email protected] Composite Film as a Fluorescence Sensor for Detection of Chilled Pork Freshness.

Biosensors (Basel) 2022 Jul 20;12(7). Epub 2022 Jul 20.

Research Center of Information Technology, Beijing Academy of Agricultural and Forestry Sciences, Beijing 100097, China.

This study presents a novel composite thin film based on rhodamine B encapsulated into MOF-5 (Metal Organic Frameworks) as a fluorescence sensor for the real-time detection of the freshness of chilled pork. The composite film can adsorb and respond to the volatile amines produced by the quality deterioration of pork during storage at 4 °C, with the fluorescence intensity of RhB decreasing over time. The quantitative model used for predicting the freshness indicator (total volatile base nitrogen) of pork was built using the fluorescence spectra (excited at 340 nm) of the [email protected] composite film combined with the partial least squares (PLS) algorithm, providing R and R values of 0.908 and 0.821 and RMSEC (root mean square error of calibration) and RMSEP (root mean square error of prediction) values of 3.435 mg/100 g and 3.647 mg/100 g, respectively. The qualitative model established by the partial least squares discriminant analysis (PLS-DA) algorithm was able to accurately classify pork samples as fresh, acceptable or spoiled, and the accuracy was 86.67%.
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http://dx.doi.org/10.3390/bios12070544DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9313163PMC
July 2022

Prognostic factors and related complications/sequalae of squamous cell carcinoma located in the gingivobuccal complex.

World J Surg Oncol 2022 Jul 26;20(1):240. Epub 2022 Jul 26.

Department of Oral and Maxillofacial-Head and Neck Oncology, Beijing Stomatological Hospital, Capital Medical University, No. 4; Tian Tan Xi Li, Dongcheng District, Beijing, 100050, P.R. China.

Background: Gingivobuccal complex (GBC) was a relatively new concept of oral subsite that was comprises of the upper and/or lower gingiva, gingival buccal sulcus, and adjacent buccal mucosa. Squamous cell carcinoma (SCC) of the GBC had a poor prognosis, with few studies analyzing this particular entity. The objective of this study was to analyze the risk factors affecting the prognosis and complications/sequalae of gingivobuccal complex cancer.

Methods: Between December 2014 and August 2019, a total of 122 patients diagnosed with primary gingivobuccal complex cancer in Beijing Stomatological Hospital, Capital Medical University were enrolled in the study. Through outpatient reviewed and telephone followed-up for 2-5 years postoperatively, postoperative relapse and complications/sequalae were assessed. The primary outcome parameter was 2-year disease-free survival.

Results: The most common central site of the tumor was the buccal mucosa (45.1%), followed by the lower gingiva (36.9%). The most diseases were pT4a (45.1%) and there was lymph node invasion (pN+) in 41.8% of patients. Moderate differentiated squamous carcinoma (77.9%) accounted for the vast majority of the histopathological differentiation. A total of 62.3% of tumors invaded the bone, while, 5.7% invaded the skin layer. Survival analysis found that 44.3% of patients experienced relapse within two years postoperatively and the mortality rate after relapse was 75.9%. Almost 60.0% of the tumors involving the maxilla and/or mandible developed relapse. Cox proportional hazards model found that pN stage (p= 0.002) and bone invasion (p= 0.007) were significant independent predictors of 2-year disease-free survival. Importantly, 63.1% of patients had postoperative (and postradiotherapy) complications/sequalae. It was noteworthy that 18 of 43 patients (41.9%) who implanted with titanium plates had hardware-related complications/sequalae, and the most of them were titanium plate exposure (61.1%).

Conclusions: Squamous cell carcinoma of the gingivobuccal complex cancer, as a new subsite worthy of attention in oral cancer, has a high complication/sequalae rate, high relapse rate and poor prognosis.

Trial Registration: Prospective, Observational, Real-world Oral Malignant Tumors Study ( clinicaltrials.gov identifier: NCT02395367). The approval of the Institutional Review Board of the Beijing Stomatological Hospital of Capital Medical University (Approval number: CMUSH-IRB-KJPJ-2015-08).
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http://dx.doi.org/10.1186/s12957-022-02708-wDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9316736PMC
July 2022

Factors influencing the professional identity of nursing interns: a cross-sectional study.

BMC Nurs 2022 Jul 25;21(1):200. Epub 2022 Jul 25.

The Third Xiangya Hospital, Central South University, Changsha, Hunan, China.

Background: Improving the professional identity of nursing intern is significant for enhancing the number of new registered nurses and easing the shortage of nursing personnel. The clinical internship is a key period for the formulation of professional identity. However, we know little about the factors influencing the nursing interns' professional identity during clinical internship. Therefore, this study explore the influencing factors of nursing interns' professional identity during clinical internship. This study will provide evidence and suggestions for generating effective strategies contributing to professional identity improvement of nursing interns.

Methods: This was a cross-sectional study. The convenience sampling was used to recruit 398 nursing interns from a teaching hospital in Hunan, China. The demographic characteristics information was collected by a self-developed questionnaire. The nursing interns' professional identity and potential influencing factors (e.g., work atmosphere, teacher capacity) were measured by questionnaires with good psychometric properties. The appropriate indicators were used for descriptive statistics, and t test, analysis of variance, Pearson's correlation analysis and multiple linear regression were used to analyse the influencing factors.

Results: In this study, the influencing factors of nursing interns' professional identity are education level, first choice of major, residential status, work atmosphere, and teacher capacity. The results showed that: (1) the nursing interns with a higher education level reported a lower level of professional identity; (2) the nursing interns whose first choice of major was not nursing discipline reported a lower level of professional identity; (3) the nursing interns live in rural areas (compared to urban areas) reported a higher level of professional identity; (4) the nursing interns in better work atmosphere reported a higher level of professional identity; (5) the nursing interns under the guidance of the teachers equipped with better teaching capacity reported a higher level of professional identity.

Conclusion: The education level, first choice of major and residential status are influence factors of nursing interns' professional identity. The nursing educators need to pay attention to nursing interns whose first choice is not nursing, and in a bachelor program, who may have a lower level of professional identity. It is crucial to enhance the nursing interns' professional identity by improve the work atmosphere and clinical teachers' capacity, to promote nursing interns to choose nursing as a profession and reduce the shortage of nursing workforce.
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http://dx.doi.org/10.1186/s12912-022-00983-2DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9310353PMC
July 2022

Targeting ACLY efficiently inhibits SARS-CoV-2 replication.

Int J Biol Sci 2022 11;18(12):4714-4730. Epub 2022 Jul 11.

State Key Laboratory of Emerging Infectious Diseases, Department of Microbiology, and Carol Yu Centre for Infection, School of Clinical Medicine, Li Ka Shing Faculty of Medicine, The University of Hong Kong, Pokfulam, Hong Kong Special Administrative Region, People's Republic of China.

The Coronavirus Disease 2019 (COVID-19) pandemic caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is the biggest public health challenge the world has witnessed in the past decades. SARS-CoV-2 undergoes constant mutations and new variants of concerns (VOCs) with altered transmissibility, virulence, and/or susceptibility to vaccines and therapeutics continue to emerge. Detailed analysis of host factors involved in virus replication may help to identify novel treatment targets. In this study, we dissected the metabolome derived from COVID-19 patients to identify key host factors that are required for efficient SARS-CoV-2 replication. Through a series of metabolomic analyses, , and investigations, we identified ATP citrate lyase (ACLY) as a novel host factor required for efficient replication of SARS-CoV-2 wild-type and variants, including Omicron. ACLY should be further explored as a novel intervention target for COVID-19.
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http://dx.doi.org/10.7150/ijbs.72709DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9305265PMC
July 2022

Simultaneous detection of Cd and Pb in food based on sensing electrode prepared by conductive carbon paper, rGO and CoZn·MOF (CP-rGO-CoZn·MOF).

Anal Chim Acta 2022 Aug 29;1220:339812. Epub 2022 Apr 29.

State Key Laboratory of Urban Water Resources & Environment, Harbin Institute of Technology, Harbin, 150090, PR China. Electronic address:

Cd and Pb are two typical metallic pollutants in food. Therefore, it is of great significance to establish a method which can simultaneously detect them. Here, an electrochemical sensor was established to simultaneously detect Cd and Pb in food samples based on sensing electrode prepared by conductive carbon paper, rGO and CoZn·MOF (CP-rGO-CoZn·MOF). Under the optimized conditions, the proposed electrochemical sensor achieves simultaneous detection of Cd and Pb low to 0.565 nM (Cd) and 0.588 nM (Pb), respectively. Further selective experiments and repetitive experiments perform well. Spiked experiments find that the recovery rates of Cd and Pb in food samples are between 97.5% to 105.9% and 94.3%-109.6%, indicating the established electrochemical sensor owns a good response to the targets. Besides, relative standard deviations (RSD) of them are no more than 5.817%, showing its potential in practical applications.
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http://dx.doi.org/10.1016/j.aca.2022.339812DOI Listing
August 2022

Eupalinolide B inhibits hepatic carcinoma by inducing ferroptosis and the ROS-ER-JNK signaling pathway.

Acta Biochim Biophys Sin (Shanghai) 2022 Jul 25. Epub 2022 Jul 25.

The People's Hospital Affiliated to Chongqing Three Gorges Medical College, Chongqing 404100, China.

Primary hepatic carcinoma is a common malignant tumor. The classic molecular targeted drug sorafenib is costly and is only effective for some patients. Therefore, it is of great clinical significance to search for new molecular targeted drugs. Eupalinolide B (EB) from Eupatorium lindleyanum DC. is used to treat chronic tracheitis in clinical practice. However, the role of EB in hepatic carcinoma is unknown. In this study, we first measure the effect of EB on tumor growth in a xenograft model and PDX model. The cell proliferation and migration are also detected in human hepatocarcinoma cell lines (SMMC-7721 and HCCLM3). Then, we investigate cell cycle, cell apoptosis, cell necrosis, cell autophagy, and ferroptosis by flow cytometry, western blot analysis and electron microscopy. The results demonstrate that EB exerts anti-proliferative activity in hepatic carcinoma by blocking cell cycle arrest at S phase and inducing ferroptosis mediated by endoplasmic reticulum (ER) stress, as well as HO-1 activation. When HO-1 is inhibited, EB-induced cell death and ER protein expression are rescued. The migration-related mechanism consists of activation of the ROS-ER-JNK signaling pathway and is not connected to ferroptosis. In summary, we first discover that EB inhibits cell proliferation and migration in hepatic carcinoma, and thus EB is a promising anti-tumor compound that can be used for hepatic carcinoma.
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http://dx.doi.org/10.3724/abbs.2022082DOI Listing
July 2022

Opposite roles of Kindlin orthologs in cell survival and proliferation.

Cell Prolif 2022 Jul 20:e13280. Epub 2022 Jul 20.

Department of Neurosciences, Lerner Research Institute, Cleveland Clinic, Cleveland, Ohio, USA.

Objective: It is unclear why adhesion-dependent cells such as epithelium undergo anoikis without anchorage, while adhesion-independent blood cells thrive in suspension. The adhesive machinery of these cells is similar, with the exception of Kindlin orthologs, Kindlin 2 (K2) and Kindlin 3 (K3). Here we address how Kindlins control cell survival and proliferation in anchorage-dependent and independent cells.

Material And Methods: To demonstrate the opposite roles of Kindlin's in cell survival we utilized in vivo and in vitro models and K3 and K2 knockdown and knockin cells. We used human lymphocytes from the K3 deficient patients in tumour model, K3 knockout and knockin macrophages and K2 knockout and knockin MEF cells for experiments in under conditions of adhesion and in suspension.

Results: Depletion of K3 promotes cell proliferation and survival of anchorage-independent cells regardless of cell attachment. In contrast, the absence of K2 in anchorage-dependent cells accelerates apoptosis and limits proliferation. K3 deficiency promotes human lymphoma growth and survival in vivo. Kindlins' interaction with paxillin, is critical for their differential roles in cell anchorage. While disruption of K2-paxillin binding leads to increased apoptosis, the lack of K3-paxillin binding has an opposite effect in adhesion-independent cells.

Conclusion: Kindlin ortologs and their interaction to cytoskeletal protein paxillin define the mechanisms of anchorage dependence. Our study identifies the key elements of the cell adhesion machinery in cell survival and tumour metastasis, proposing possible targets for tumour treatment.
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http://dx.doi.org/10.1111/cpr.13280DOI Listing
July 2022

Dexmedetomidine pretreatment alleviates cerebral ischemia/reperfusion injury by inhibiting neuroinflammation through the JAK2/STAT3 pathway.

Braz J Med Biol Res 2022 13;55:e12145. Epub 2022 Jul 13.

Department of Anesthesiology, Hebei General Hospital, Shijiazhuang, Hebei, China.

Dexmedetomidine (DEX) is known to provide neuroprotection against cerebral ischemia and reperfusion injury (CIRI), but the exact mechanisms remain unclear. This study was conducted to investigate whether DEX pretreatment conferred neuroprotection against CIRI by inhibiting neuroinflammation through the JAK2/STAT3 signaling pathway. Middle cerebral artery occlusion (MCAO) was performed to establish a cerebral ischemia/reperfusion (I/R) model. Specific-pathogen-free male Sprague-Dawley rats were randomly divided into Sham, I/R, DEX, DEX+IL-6, and AG490 (a selective inhibitor of JAK2) groups. The Longa score, TTC staining, and HE staining were used to evaluate brain damage. ELISA was used to exam levels of TNF-α. Western blotting was used to assess the levels of JAK2, phosphorylated-JAK2 (p-JAK2), STAT3, and phosphorylated-STAT3 (p-STAT3). Our results suggested that both pretreatment with DEX and AG490 decreased the Longa score and cerebral infarct areas following cerebral I/R. After treatment with IL-6, the effects of DEX on abrogating these pathological changes were reduced. HE staining revealed that I/R-induced neuronal pathological changes were attenuated by DEX application, consistent with the AG490 group. However, these effects of DEX were abolished by IL-6. Furthermore, TNF-α levels were significantly increased in the I/R group, accompanied by an increase in the levels of the p-JAK2 and p-STAT3. DEX and AG490 pretreatment down-regulated the expressions of TNF-α, p-JAK2, and p-STAT3. In contrast, the down-regulation of TNF-α, p-JAK2, and p-STAT3 induced by DEX was reversed by IL-6. Collectively, our results indicated that DEX pretreatment conferred neuroprotection against CIRI by inhibiting neuroinflammation via negatively regulating the JAK2/STAT3 signaling pathway.
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http://dx.doi.org/10.1590/1414-431X2022e12145DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9296126PMC
July 2022

Panoramic comparison between NK cells in healthy and cancerous liver through single-cell RNA sequencing.

Cancer Biol Med 2022 Jul 21. Epub 2022 Jul 21.

The CAS Key Laboratory of Innate Immunity and Chronic Disease, School of Basic Medical Sciences, Division of Life Sciences and Medicine, University of Science and Technology of China, Hefei 230027, China.

Objective: NK cells play crucial roles in the immune defense mechanisms against viral infections and transformed cells. However, the developmental progression, transcriptomic landscape, and functional subtypes of liver NK cells are not well defined. Hepatocellular carcinoma (HCC) accounts for approximately 80% of primary liver cancer worldwide, yet the biological characteristics of NK cells in the HCC environment are unclear. Therefore, we aimed to determine these cells' roles in tumorigenesis and prognosis.

Methods: We compared the single-cell RNA sequencing profiles of NK cells purified from blood ( = 1), healthy liver tissues ( = 3), HCC tumor tissues ( = 4), and peritumor liver tissues ( = 1) to identify NK cell subsets. Furthermore, we performed bioinformatics analysis by using The Cancer Genome Atlas (TCGA) data to identify prognostic biomarkers simultaneously overexpressed in the blood and tumor tissues of patients with HCC.

Results: Transcriptomic analysis revealed 5 NK cell subsets (L1-NK-CD56, L2-NK-CD56, L3-NK-HLA, L4-LrNK-FCGR3A, and L5-LrNK-XCL1) in the healthy liver tissues. However, the transitional L3 subset and the CXCR6CD16 L4 subset with strong anti-tumor activity were absent in the HCC and peritumor liver tissues. Furthermore, 4 common prognosis-associated genes (, , , and ) were significantly overexpressed in the paired tumor tissue and blood.

Conclusions: Our study revealed 5 specific subsets of NK cells in healthy human liver tissues. However, only 3 of the 5 NK cell subsets were present in HCC and peritumor tissues. The cytotoxic NK cell subsets were absent in HCC tissues. Furthermore, we identified 4 potential non-invasive prognostic biomarkers in patients with HCC.
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http://dx.doi.org/10.20892/j.issn.2095-3941.2022.0050DOI Listing
July 2022

[Pharmacoeconomic evaluation of Xiangju Capsules in treatment of chronic rhinosinusitis].

Zhongguo Zhong Yao Za Zhi 2022 Jul;47(14):3950-3955

Institute of Basic Research in Clinical Medicine, China Academy of Chinese Medical Sciences Beijing 100700, China.

Chronic rhinosinusitis refers to the non-specific inflammation that occurs in the nasal mucosa and sinuses, with clinical manifestations of mucopurulent nasal discharge, nasal congestion, hyposmia or anosmia, and head and face swelling pain.This disease has a long course and is prone to recurrence, which seriously affects the physical and mental health and quality of life of patients.Xiangju Capsules, prepared from infructescence of Platycarya strobilacea(without seeds), Prunellae Spica, Magnoliae Flos, Chuan-xiong Rhizoma, Angelicae Dahuricae Radix, Chrysanthemi Indici Flos, Astragali Radix, etc., are effective in dispelling wind by pungency and dispersing, clearing heat, and relieving orifices, and has good efficacy and safety in the clinical treatment of acute and chronic rhinosinusitis and rhinitis.From the perspective of the health system, an economic evaluation model was constructed based on the Meta-analysis of the effectiveness of Xiangju Capsules in the treatment of chronic rhinosinusitis to discuss the economic efficiency of Xiangju Capsules in combination with conventional treatment or conventional treatment alone in patients with chronic rhinosinusitis.The model simulation results showed that 53 days(average course of treatment) after treatment, Xiangju Capsules combined with conventional treatment had higher cost and higher output, with an incremental cost-effectiveness ratio of CNY 263.71, about 0.82% of per capita disposable income.As revealed by sensitivity analysis results, the research results were robust.As indicated by the findings of this study, Xiangju Capsules combined with conventional treatment for chronic rhinosinusitis patients were more economical than conventional treatment alone based on the assumption that the per capita disposable income of Chinese people in 2020 was the threshold of patients' willingness to pay.
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http://dx.doi.org/10.19540/j.cnki.cjcmm.20220415.501DOI Listing
July 2022

Influence of the Injection Scheme on the Enhanced Oil Recovery Ability of Heterogeneous Phase Combination Flooding in Mature Waterflooded Reservoirs.

ACS Omega 2022 Jul 28;7(27):23511-23520. Epub 2022 Jun 28.

College of Petroleum Engineering, Yangtze University, Wuhan 430100, China.

With the maturity of waterflooded reservoirs, owing to serious heterogeneity, the fluid will channel through the thief zone, leading to considerable remaining oil unrecovered in the upswept area. To further enhance oil recovery (EOR) after waterflooding, the heterogeneous phase combination flooding (HPCF) was composed of a polymer, branched-preformed particle gel (B-PPG), and surfactant. For the sake of improving the economic efficiency, the influence of the injection scheme on the EOR of HPCF with an equal chemical agent cost was investigated by sand-pack flooding experiments. Then, visual plate sand-pack model flooding experiments were performed to study the swept area of HPCF under different injection schemes. Results demonstrated that the total EOR of HPCF under different injection schemes ranged from 33.5 to 39.3%. Moreover, the EOR of HPCF under the alternation injection (AI) scheme was the highest, followed by the concentration step change injection (CI) scheme, and that of the simultaneous injection (SI) scheme was the least. The visual flooding experimental results showed that the swept area of HPCF after waterflooding under the AI scheme was higher than that of the SI. Moreover, in view of qualitative analysis of remaining oil distribution, the EOR of AI of HPCF was higher than that of SI, which was consistent with the parallel sand-pack flooding results.
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http://dx.doi.org/10.1021/acsomega.2c02007DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9281330PMC
July 2022

Corrigendum: Diet and Culture Among Chinese Patients Undergoing Hemodialysis: A Qualitative Study.

Front Nutr 2022 29;9:967573. Epub 2022 Jun 29.

Department of Fundamentals of Nursing, Medical School, Nantong University, Nantong, China.

[This corrects the article DOI: 10.3389/fnut.2022.876179.].
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http://dx.doi.org/10.3389/fnut.2022.967573DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9282167PMC
June 2022

Spatio-Temporal Modification of Lignin Biosynthesis in Plants: A Promising Strategy for Lignocellulose Improvement and Lignin Valorization.

Front Bioeng Biotechnol 2022 1;10:917459. Epub 2022 Jul 1.

Biofuels Institute, School of the Environment and Safety Engineering, Jiangsu University, Zhenjiang, China.

Lignin is essential for plant growth, structural integrity, biotic/abiotic stress resistance, and water transport. Besides, lignin constitutes 10-30% of lignocellulosic biomass and is difficult to utilize for biofuel production. Over the past few decades, extensive research has uncovered numerous metabolic pathways and genes involved in lignin biosynthesis, several of which have been highlighted as the primary targets for genetic manipulation. However, direct manipulation of lignin biosynthesis is often associated with unexpected abnormalities in plant growth and development for unknown causes, thus limiting the usefulness of genetic engineering for biomass production and utilization. Recent advances in understanding the complex regulatory mechanisms of lignin biosynthesis have revealed new avenues for spatial and temporal modification of lignin in lignocellulosic plants that avoid growth abnormalities. This review explores recent work on utilizing specific transcriptional regulators to modify lignin biosynthesis at both tissue and cellular levels, focusing on using specific promoters paired with functional or regulatory genes to precisely control lignin synthesis and achieve biomass production with desired properties. Further advances in designing more appropriate promoters and other regulators will increase our capacity to modulate lignin content and structure in plants, thus setting the stage for high-value utilization of lignin in the future.
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http://dx.doi.org/10.3389/fbioe.2022.917459DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9283729PMC
July 2022

Impact of the National Reimbursement Drug List Negotiation Policy on Accessibility of Anticancer Drugs in China: An Interrupted Time Series Study.

Front Public Health 2022 1;10:921093. Epub 2022 Jul 1.

Department of Health Policy, Health Management College, Harbin Medical University, Harbin, China.

Objective: Since 2016, the Chinese government has been regularly implementing the National Reimbursement Drug List Negotiation (NRDLN) to improve the accessibility of drugs. In the second round of NRDLN in July 2017, 18 anticancer drugs were included. This study analyzed the impact of the NRDLN on the accessibility of these 18 anticancer drugs in China.

Methods: National hospital procurement data were collected from 2015 to 2019. As measurements of drug accessibility, monthly average of drug availability or defined daily dose cost (DDDc) was calculated. Interrupted time series (ITS) analysis was employed to evaluate the impact of NRDLN on drug accessibility. Multilevel growth curve models were estimated for different drug categories, regions or levels of hospitals.

Results: The overall availability of 18 anticancer drugs increased from about 10.5% in 2015 to slightly over 30% in 2019. The average DDDc dropped from 527.93 CNY in 2015 to 401.87 CNY in 2019, with a reduction of 23.88%. The implementation of NRDLN was associated with higher availability and lower costs for all 18 anticancer drugs. We found an increasing level in monthly drug availability (β = 2.1126), which ascended more sharply after the implementation of NRDLN (β = 0.3656). There was a decreasing level in DDDc before July 2017 (β = -108.7213), together with a significant decline in the slope associated with the implementation of NRDLN (β = -4.8332). Compared to Traditional Chinese Medicines, the availability of Western Medicines was higher and increased at a higher rate (β = 0.4165 vs. 0.1108). Drug availability experienced a larger instant and slope increase in western China compared to other regions, and in secondary hospitals than tertiary hospitals. Nevertheless, regional and hospital-level difference in the effect of NRDLN on DDDc were less evident.

Conclusion: The implementation of NRDLN improves the availability and reduces the cost of some anticancer drugs in China. It contributes to promoting accessibility of anticancer drugs, as well as relieving regional or hospital-level disparities. However, there are still challenges to benefit more patients sufficiently and equally. It requires more policy efforts and collaborative policy combination.
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http://dx.doi.org/10.3389/fpubh.2022.921093DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9283976PMC
July 2022

Machine learning is an effective method to predict the 90-day prognosis of patients with transient ischemic attack and minor stroke.

BMC Med Res Methodol 2022 07 16;22(1):195. Epub 2022 Jul 16.

Department of Neurology, Beijing Tiantan Hospital, Capital Medical University, No.119 South 4th Ring West Road, Fengtai District, Beijing, 100070, China.

Objective: We aimed to investigate factors related to the 90-day poor prognosis (mRS≥3) in patients with transient ischemic attack (TIA) or minor stroke, construct 90-day poor prognosis prediction models for patients with TIA or minor stroke, and compare the predictive performance of machine learning models and Logistic model.

Method: We selected TIA and minor stroke patients from a prospective registry study (CNSR-III). Demographic characteristics,smoking history, drinking history(≥20g/day), physiological data, medical history,secondary prevention treatment, in-hospital evaluation and education,laboratory data, neurological severity, mRS score and TOAST classification of patients were assessed. Univariate and multivariate logistic regression analyses were performed in the training set to identify predictors associated with poor outcome (mRS≥3). The predictors were used to establish machine learning models and the traditional Logistic model, which were randomly divided into the training set and test set according to the ratio of 70:30. The training set was used to construct the prediction model, and the test set was used to evaluate the effect of the model. The evaluation indicators of the model included the area under the curve (AUC) of the discrimination index and the Brier score (or calibration plot) of the calibration index.

Result: A total of 10967 patients with TIA and minor stroke were enrolled in this study, with an average age of 61.77 ± 11.18 years, and women accounted for 30.68%. Factors associated with the poor prognosis in TIA and minor stroke patients included sex, age, stroke history, heart rate, D-dimer, creatinine, TOAST classification, admission mRS, discharge mRS, and discharge NIHSS score. All models, both those constructed by Logistic regression and those by machine learning, performed well in predicting the 90-day poor prognosis (AUC >0.800). The best performing AUC in the test set was the Catboost model (AUC=0.839), followed by the XGBoost, GBDT, random forest and Adaboost model (AUCs equal to 0.838, 0, 835, 0.832, 0.823, respectively). The performance of Catboost and XGBoost in predicting poor prognosis at 90-day was better than the Logistic model, and the difference was statistically significant(P<0.05). All models, both those constructed by Logistic regression and those by machine learning had good calibration.

Conclusion: Machine learning algorithms were not inferior to the Logistic regression model in predicting the poor prognosis of patients with TIA and minor stroke at 90-day. Among them, the Catboost model had the best predictive performance. All models provided good discrimination.
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http://dx.doi.org/10.1186/s12874-022-01672-zDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9287991PMC
July 2022

Clinical value of YKL-40 in patients with polymyositis/dermatomyositis: A cross-sectional study and a systematic review.

J Clin Lab Anal 2022 Jul 15:e24605. Epub 2022 Jul 15.

Department of Rheumatology and Immunology, West China Hospital, Sichuan University, Chengdu, China.

Introduction: We performed a cross-sectional study to investigate the clinical usefulness of YKL-40 in patients with dermatomyositis (DM) and conducted a systematic review to summarize the clinical value of YKL-40 in patients with polymyositis (PM)/DM.

Materials And Methods: A cross-sectional study and a systematic review were performed to study the clinical value of YKL-40 in patients with PM/DM. Serum YKL-40 level was detected using enzyme-linked immunosorbent assay, and its association with clinical and laboratory parameters was analyzed. In the systematic review, electronic databases of OVID Embase, OVID Medline, and web of science were searched to collect studies that reported clinical use of YKL-40 in patients with PM/DM.

Results: In the cross-sectional study, serum YKL-40 level was higher in patients with DM than in healthy controls (median [interquartile range]: 84.09 [52.72-176.4] ng/ml versus 27.37 [12.30-53.58] ng/ml, p < 0.0001). Serum levels of YKL-40 were associated with the course of DM (r = -0.469, p < 0.001), CRP (r = 0.303, p = 0.043), CK (r = 0.263, p = 0.037), and global disease activity (r = 0.628, p < 0.001). The area under the ROC curve was 0.835 (95% confidence interval 0.751-0.920). In the systematic review, a total of four studies were included with moderate to high quality. Serum level of YKL-40 has the possibility for diagnosing PM/DM, identifying PM/DM patients with interstitial lung disease (ILD) or rapid progress ILD, and predicting death.

Conclusion: Serum YKL-40 level is a possible useful biomarker for PM/DM diagnosis and may be used to predict prognosis.
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http://dx.doi.org/10.1002/jcla.24605DOI Listing
July 2022

Prediction of potential severe coronavirus disease 2019 patients based on CT radiomics: A retrospective study.

Med Phys 2022 Jul 15. Epub 2022 Jul 15.

Department of Radiology, Zhongnan Hospital of Wuhan University, Wuhan, China.

Purpose: Coronavirus disease 2019 (COVID-19) is a recently declared worldwide pandemic. Triaging of patients into severe and non-severe could further help in targeted management. "Potential severe patients" is a category of patients who did not have severe symptoms at their initial diagnosis, but eventually progressed to be severe patients and are easily overlooked in the early stage. This work aimed to develop and evaluate a CT-based radiomics signature for the prediction of these potential severe COVID-19 patients.

Methods: One hundred fifty COVID-19 patients were enrolled and randomly divided into cross-validation and independent test sets. First, their clinical characteristics were screened using the univariate and multivariate logistic regression step by step. Then, radiomics features were extracted from the lesions on their chest CT images. Subsequently, the inter- and intra-class correlation coefficients (ICC) analysis, minimum-redundancy maximum-relevance (mRMR) selection, and the least absolute shrinkage and selection operator (LASSO) algorithm were used step by step for feature selection and construction of a radiomics signature. Finally, the screened clinical risk factors and constructed radiomics signature were combined for the combined model and Radiomics+Clinics nomogram construction. The predictive performance of the Radiomics and Combined models were evaluated and compared using receiver operating characteristic curve (ROC) analysis, Hosmer-Lemeshow test and Delong test.

Results: Clinical characteristics analysis resulted in the screening of five clinical risk factors. The combination of ICC, mRMR, and LASSO methods resulted in the selection of ten radiomics features, which made up of the radiomics signature. The differences in the radiomics signature between the potential severe and non-severe groups in cross-validation set and test sets were both p < 0.001. All Radiomics and Combined models showed a very good predictive performance with the accuracy and AUC of nearly or above 0.9. Additionally, we found no significant difference in the predictive performance between these two models.

Conclusions: A CT-based radiomics signature for the prediction of potential severe COVID-19 patients was constructed and evaluated. Constructed Radiomics and Combined model showed good feasibility and accuracy. The Radiomics+Clinical nomogram, acted as a useful tool, may assist clinicians to better identify potential severe cases to target their management in the COVID-19 pandemic prevention and control.
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http://dx.doi.org/10.1002/mp.15841DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9349830PMC
July 2022

Mediating effects of academic self-efficacy and smartphone addiction on the relationship between professional attitude and academic burnout in nursing students: A cross-sectional study.

Nurse Educ Today 2022 Sep 8;116:105471. Epub 2022 Jul 8.

Department of Nursing, Wannan Medical College, Anhui, China. Electronic address:

Background: Academic burnout is a common issue that interferes with the role shift from nursing students to qualified nurses and aggravates a shortage of workforce in global healthcare system. According to the Areas of Worklife Scale, developed by Maslach and Leiter, there are six dimensions that encompass the major antecedents of burnout. Therefore, it's well worth an exploration to understand academic burnout and its' underlying mechanism based on the theoretical instrument. Specifically, academic burnout, professional attitude, academic self-efficacy and smartphone addiction were selected as observation variables after widely literature review.

Objective: The purpose of this study is to examine academic burnout and figure out the relationships between academic burnout, professional attitude, academic self-efficacy and smartphone addiction among nursing students.

Design: A cross-sectional study.

Setting: The study was conducted in a nursing department in Anhui province in eastern China.

Participants: 1445 nursing students.

Methods: Academic burnout, professional attitude, academic self-efficacy and smartphone addiction of participants were measured using online questionnaires with a supportive platform called "wenjuanxing". Data were analyzed using descriptive statistics, Pearson's correlations, and path analysis.

Results: Of all the participants, 44.26 % had a certain degree of academic burnout. Professional attitude and academic self-efficacy were negatively related to academic burnout. Smartphone addiction was positively associated with academic burnout. Meanwhile, academic self-efficacy and smartphone addiction partly mediated the effect of professional attitude on academic burnout.

Conclusion: The compound strategies targeted at boosting positive professional attitude, fostering academic self-efficacy, and controlling the smartphone addiction are warranted for decreasing academic burnout.
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http://dx.doi.org/10.1016/j.nedt.2022.105471DOI Listing
September 2022

The full-length genome sequence of a novel amalgavirus in Lilium spp. in China.

Arch Virol 2022 Jul 14. Epub 2022 Jul 14.

Hubei Key Laboratory of Plant Pathology, College of Plant Science and Technology, Huazhong Agricultural University, Wuhan, China.

We report for the first time the complete genome sequence of a novel amalgavirus, tentatively designated as 'lily amalgavirus 1' (LAV-1), isolated from Lilium spp. in China. LAV-1 is a 3448-nt double-stranded RNA virus that encodes two putative proteins. Open reading frame 1 (ORF1) encodes a 394-aa protein with unknown function. ORF2 encodes a putative RNA-dependent RNA polymerase (RdRp) of 895 aa. The two ORFs putatively encode a '1 + 2' fusion protein generated by a '+1' programmed ribosomal frameshift (PRF). BLASTp analysis revealed that the complete genome sequence of LAV-1 shares 48.23-59.80% sequence identity (query sequence coverage > 77%) with those of members of the genus Amalgavirus, with the highest nucleotide sequence identity of 59.80% with that of Allium cepa amalgavirus 1 (query sequence coverage, 87%). The genome structure, phylogenetic relationships, and sequence similarities to other plant amalgaviruses suggest that LAV-1 is a new member of the genus Amalgavirus.
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http://dx.doi.org/10.1007/s00705-022-05523-6DOI Listing
July 2022

Impact of socioeconomic status and health risk on fall inequality among older adults.

Health Soc Care Community 2022 Jul 14. Epub 2022 Jul 14.

School of Political Science and Public Administration, Wuhan University, Wuhan, China.

Socio-economic status (SES) is one of the important indicators reflecting individual social participation and resource allocation, and it plays an important role in individual health shock. Health shock indicates the body being in a non-healthy state, such as illness, injury and death. This study used data from the China Health and Retirement Longitudinal Study conducted in 2013, 2015 and 2018 and utilised the panel logit model, causal step-by-step analysis and path analysis to empirically test the impact of SES on fall inequality among the elderly and the mediating effect of health shock. The results demonstrated obvious group inequality in the fall risk among older adults, the core of which was the impact of SES on health, causing health inequality and affecting fall inequality. The activities of daily living and pain rate of the high-income group were 61.16% and 28.69%, respectively, that of the low-income group. The evaluation of good sight and hearing were 3.6833 and 3.8572 times, respectively, that of the low-income group. The non-depressive status was 38.4638 times of the low-income group. The path effect confirmed the mediating role of health shock. Therefore, this study concluded that SES had an important impact on the risk of falls among the elderly, and this impact was mainly from health shock. This study proposed that to reduce the incidence of falls among the elderly, differences in health shock risks among the elderly with different SES must be actively monitored and accurate and effective policy interventions should be implemented from the level of group heterogeneity.
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http://dx.doi.org/10.1111/hsc.13908DOI Listing
July 2022

Factors contributing to the high prevalence of multidrug-resistance/Rifampicin-resistance in patients with tuberculosis: an epidemiological cross sectional and qualitative study from Khabarovsk krai region of Russia.

BMC Infect Dis 2022 Jul 13;22(1):612. Epub 2022 Jul 13.

Health Management College, Social Medicine Department, Harbin Medical University, 157 Baojian Road, Nangang District, Harbin, Heilongjiang, 10081, People's Republic of China.

Background: Growing prevalence of multidrug-resistant/Rifampicin-resistant tuberculosis (MDR/RR-TB; resistance to Isoniazid and Rifampicin/Isolated resistance to Rifampicin) is putting in jeopardy the WHO End TB strategy. This study aimed to identify factors contributing to the high prevalence of MDR/RR-TB in Khabarovsk krai region of Russia.

Methods: A cross-sectional retrospective study was conducted, analyzing clinical, demographic, and drug susceptibility testing data on 1440 patients. As a source of raw data, the national electronic TB surveillance system was used. Anonymous data was collected on every patient diagnosed with TB in all healthcare facilities of the region from January 2018 to December 2019. Only patients with proven excretion of m. tuberculosis were included in the study. Factors associated with MDR/RR-TB were identified through logistic regression analysis, in conjunction with in-depth interviews with eight patients, five healthcare managers and five doctors.

Findings: 2661 patients were identified with TB, 1440 were incorporated in the study based on inclusion criteria. Of these, 618 (42.9%) were identified with MDR/RR-TB. Patients with a history of imprisonment were 16.53 times (95% CI 5.37 to 50.88,) more likely to have MDR/RR-TB, whereas re-treatment patients were 2.82 times (95% CI 2.16 to 3.66) more likely to have MDR/RR-TB. Other influencing factors included presence of disability (AOR is 2.32, 95% CI 1.38 to 3.89), cavitary disease (AOR is 1.76, 95% CI 1.37 to 2.25), and retirement status (AOR 0.65, 95% CI 0.43 to 0.98, p = 0.042). Poor patient knowledge and understanding of the disease, progressive weariness of prolonged TB treatment, and inability hospitalize infectious patients without their consent were perceived by the interviewees as major influencing factors.

Conclusions: Incarceration and treatment history, regardless of outcome, were identified as major factors influencing MDR/RR-TB prevalence. It is essential for the TB care system to eliminate legal loopholes, which deprive doctors of means to enforce quarantine procedures and epidemiological surveillance on infected patients, former and current inmates. Increasing people's awareness of TB, early detection and appropriate treatment of patients with TB are needed for successfully combating MDR/RR-TB.
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http://dx.doi.org/10.1186/s12879-022-07598-7DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9281178PMC
July 2022

Postoperative Complications of Free Flap Reconstruction in Moderate-Advanced Head and Neck Squamous Cell Carcinoma: A Prospective Cohort Study Based on Real-World Data.

Front Oncol 2022 24;12:792462. Epub 2022 Jun 24.

Department of Oral and Maxillofacial-Head and Neck Oncology, Beijing Stomatological Hospital, Capital Medical University, Beijing, China.

Background: Postoperative complications (POCs) of moderate-advanced head and neck squamous cell carcinoma (HNSCC) after free flap reconstruction have received little attention. We investigated the risk factors that lead to POCs and their impact on management and prognosis.

Patients And Methods: A single-center, prospective cohort study was conducted at Beijing Stomatological Hospital on primary HNSCC patients treated between 2015 and 2020.

Results: In total, 399 consecutive HNSCC patients who underwent radical resection of the primary tumor and free flap reconstruction were enrolled in this study, 155(38.8%) experienced POCs. The occurrence of POCs directly led to worse short-term outcomes and poorer long-term overall survival (P=0.0056). Weight loss before the operation (P=0.097), Tumor site (P=0.002), stage T4b (P=0.016), an ACE-27 index of 2-3 (P=0.040), operation time≥8h (P=0.001) and Clindamycin as antibiotic prophylaxis (P=0.001) were significantly associated with POCs.

Conclusions: The occurrence of POCs significantly leads to worse short-term outcomes and increases the patients' burden.
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http://dx.doi.org/10.3389/fonc.2022.792462DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9263716PMC
June 2022
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