Publications by authors named "Huan Lin"

137 Publications

The relationship between different types of caries and periodontal disease severity in middle-aged and elderly people: findings from the 4th National Oral Health Survey of China.

BMC Oral Health 2021 May 3;21(1):229. Epub 2021 May 3.

Hospital of Stomatology, Guanghua School of Stomatology, Sun Yat-Sen University, 56 West Lingyuan Road, Guangzhou, 510000, People's Republic of China.

Background: The relationship between dental caries and periodontal disease is still controversial. The objective of this study was to explore the relationship between different types of caries and periodontal disease severity in middle-aged and elderly people in China.

Methods: The study population consisted of 4407 middle-aged and 4117 elderly subjects. Caries were divided into the following three types: type A, crown caries; type B, lesions involving both the crown and root, representing mixed-type caries; and type C, root caries. These three types together represent the overall caries situation, which we call type ABC. Caries were quantitated by decayed and filled teeth (DFT index). Periodontitis was evaluated by clinical attachment loss.

Results: Middle-aged people with periodontitis had a significant association with DFT type B (OR: 1.21, 95% CI 1.17-1.25) and type C (OR: 1.40, 95% CI 1.24-1.56). Elderly people with periodontitis had a significant association with DFT type C (OR: 1.28, 95% CI 1.21-1.35).

Conclusions: In China, caries types B/C were positively correlated with periodontitis in the middle-aged group, and only caries type C was positively correlated with periodontitis in the elderly group.
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http://dx.doi.org/10.1186/s12903-021-01585-1DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8094505PMC
May 2021

The in-plane structure domain size of nm-thick MoSe uncovered by low-momentum phonon scattering.

Nanoscale 2021 Apr;13(16):7723-7734

Department of Mechanical Engineering, Iowa State University, Ames, IA 50011, USA.

Although 2D materials have been widely studied for more than a decade, very few studies have been reported on the in-plane structure domain (STD) size even though such a physical property is critical in determining the charge carrier and energy carrier transport. Grazing incidence X-ray diffraction (XRD) can be used for studying the in-plane structure of very thin samples, but it becomes more challenging to study few-layer 2D materials. In this work the nanosecond energy transport state-resolved Raman (nET-Raman) technique is applied to resolve this key problem by directly measuring the thermal reffusivity of 2D materials and determining the residual value at the 0 K-limit. Such a residual value is determined by low-momentum phonon scattering and can be directly used to characterize the in-plane STD size of 2D materials. Three suspended MoSe2 (15, 50 and 62 nm thick) samples are measured using nET-Raman from room temperature down to 77 K. Based on low-momentum phonon scattering, the STD size is determined to be 58.7 nm and 84.5 nm for 50 nm and 62 nm thick samples, respectively. For comparison, the in-plane structure of bulk MoSe2 that is used to prepare the measured nm-thick samples is characterized using XRD. It uncovers crystallite sizes of 64.8 nm in the (100) direction and 121 nm in the (010) direction. The STD size determined by our low momentum phonon scattering is close to the crystallite size determined by XRD, but still shows differences. The STD size by low-momentum phonon scattering is more affected by the crystallite sizes in all in-plane directions rather than that by XRD that is for a specific crystallographic orientation. Their close values demonstrate that during nanosheet preparation (peeling and transfer), the in-plane structure experiences very little damage.
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http://dx.doi.org/10.1039/d0nr09099aDOI Listing
April 2021

Dual activation of Hedgehog and Wnt/β-catenin signaling pathway caused by downregulation of SUFU targeted by miRNA-150 in human gastric cancer.

Aging (Albany NY) 2021 Apr 12;13(7):10749-10769. Epub 2021 Apr 12.

Guangdong Key Laboratory for Genome Stability and Disease Prevention, Department of Pathology, Shenzhen University School of Medicine, Shenzhen 518060, Guangdong, P.R. China.

Mounting evidence has shown that miRNA-150 expression is upregulated in gastric cancer (GC) and is associated with gastric carcinogenesis, but the underlying oncogenic mechanism remains elusive. Here, we discovered that miRNA-150 targets the tumor suppressor SUFU to promote cell proliferation, migration, and the epithelial-mesenchymal transition (EMT) via the dual activation of Hedgehog (Hh) and Wnt signaling. MiRNA-150 was highly expressed in GC tissues and cell lines, and the level of this miRNA was negatively related to that of SUFU. In addition, both the miRNA-150 and SUFU levels were associated with tumor differentiation. Furthermore, miRNA-150 activated GC cell proliferation and migration . We found that miRNA-150 inhibitors repressed not only Wnt signaling by promoting cytoplasmic β-catenin localization, but also repressed Hh signaling and EMT. MiRNA-150 inhibition also resulted in significant tumor volume reductions , suggesting the potential application of miRNA-150 inhibitors in GC therapy. The expression of genes downstream of Hh and Wnt signaling was also reduced in tumors treated with miRNA-150 inhibitors. Notably, anti-SUFU siRNAs rescued the inhibitory effects of miRNA-150 inhibitors on Wnt signaling, Hh activation, EMT, cell proliferation, cell migration, and colony formation. Taken together, these findings indicate that miRNA-150 is oncogenic and promotes GC cell proliferation, migration, and EMT by activating Wnt and Hh signaling via the suppression of SUFU expression.
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http://dx.doi.org/10.18632/aging.202895DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8064165PMC
April 2021

Effects of taillight shape on conspicuity of vehicles at night.

Appl Ergon 2021 May 18;93:103361. Epub 2021 Jan 18.

Ningbo Research Institute, Zhejiang University, Ninbo, 315100, People's Republic of China.

Taillight shape in a vehicle provides an essential lighting signal that enables the vehicle to be seen from the rear at night, thereby preventing rear-end crashes. This study aims to investigate the effects of taillight shape on vehicle conspicuity, and proposes ergonomic taillight shape solutions to vehicle designers and manufacturers. Two complementary experiments were conducted to examine three types of taillight shapes at three design levels. The first experiment was designed to investigate the detection speed of a driver and the fixation duration and fixation counts on leading vehicles with different taillight shapes, based on an eye-tracking methodology. The second experiment was designed to investigate the dynamic visual searching performance of a trailing driver for leading vehicles with different taillight shapes, based on a visual search task. The experimental results indicated that a long line-shaped taillight (striplight) was the optimal ergonomic solution for enhancing vehicle conspicuity. Vehicles with an enclosed contour-shaped taillight were more salient than those with an open contour-shaped taillight. Moreover, the experience and gender of the driver and the vehicle-observer distance were found to be closely related to vehicle conspicuity, and therefore, must be considered by vehicle designers when applying a specific taillight shape design. This study provides insights into the taillight shape design that not only aid vehicle designers or manufacturers in enhancing vehicle safety but also enable potential vehicle buyers to choose a safe lighting system.
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http://dx.doi.org/10.1016/j.apergo.2021.103361DOI Listing
May 2021

CD166 promotes cancer stem cell-like phenotype via the EGFR/ERK1/2 pathway in the nasopharyngeal carcinoma cell line CNE-2R.

Life Sci 2021 Feb 29;267:118983. Epub 2020 Dec 29.

Department of Radiation Oncology, Guangxi Medical University Cancer Hospital, Nanning, Guangxi, PR China; Wuming Hospital of Guangxi Medical University, Nanning, Guangxi, PR China; Key Laboratory of Early Prevention and Treatment for Regional High-Incidence-Tumor, Guangxi Medical University, Ministry of Education, Nanning, Guangxi, PR China. Electronic address:

Aims: The present study aimed to investigate the role and underlying mechanisms of CD166 in cancer stem cell-like (CSCs) phenotype of the radioresistant nasopharyngeal carcinoma cell CNE-2R.

Main Methods: Established CD166-shRNA- CNE-2R cell line by lentivirus-mediated silencing CD166. Then, CSC-related genes mRNAs and proteins, and EGFR/ERK1/2 signaling pathway were detected using RT-PCR and western blot. Sphere formation assay was performed to evaluate the sphere formation capacity in CD166-shRNA- CNE-2R cells. The tumorigenesis ability in vivo was examined in nude mice mode.

Key Findings: Downregulation of CD166 inhibited the expression of the CSC-related genes, pEGFR and pERK in vitro and vivo. The capacity to form spheres and tumorigenesis was significantly decreased in CD166-shRNA cells. Furthermore, EGF-stimulated CD166-shRNA cells exhibited an increase in CSC-like traits by activating EGFR/ERK1/2 signaling.

Significance: CD166 induced CSCs formation by activating the EGFR/ERK1/2 signaling pathway in nasopharyngeal carcinoma, which may serve as a critical molecular target for NPC therapeutic strategies.
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http://dx.doi.org/10.1016/j.lfs.2020.118983DOI Listing
February 2021

Germline variation networks in the PI3K/AKT pathway corresponding to familial high-incidence lung cancer pedigrees.

BMC Cancer 2020 Dec 9;20(1):1209. Epub 2020 Dec 9.

Guangdong Lung Cancer Institute, Guangdong Provincial People's Hospital and Guangdong Academy of Medical Sciences, 106, Zhongshan Er Rd, Guangzhou, 510080, China.

Background: There were scarcely germline variants of familial lung cancer (LC) identified. We conducted an study with whole-exome sequencing of pedigrees with familial lung cancer to analyze the potential genetic susceptibility.

Methods: Probands with the highest hereditary background were identified by our large-scale epidemiological study and five ones were enrolled as a learning set. The germline SNPs (single-nucleotide polymorphisms) of other five similar probands, four healthy individuals in the formerly pedigrees and three patients with sporadic LC were used as a validation set, controlled by three healthy individuals without family history of any cancer. The network of mutated genes was generated using STRING-DB and visualized using Cytoscape.

Results: Specific and shared somatic mutations and germline SNPs were not the shared cause of familial lung cancer. However, individual germline SNPs showed distinct protein-protein interaction network patterns in probands versus healthy individuals and patients with sporadic lung cancer. SNP-containing genes were enriched in the PI3K/AKT pathway. These results were validated in the validation set. Furthermore, patients with familial lung cancer were distinguished by many germline variations in the PI3K/AKT pathway by a simple SVM classification method. It is worth emphasizing that one person with many germline variations in the PI3K/AKT pathway developed lung cancer during follow-up.

Conclusions: The phenomenon that the enrichments of germline SNPs in the PI3K/AKT pathway might be a major predictor of familial susceptibility to lung cancer.
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http://dx.doi.org/10.1186/s12885-020-07528-3DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7724858PMC
December 2020

The role of the cholinergic anti-inflammatory pathway in septic cardiomyopathy.

Int Immunopharmacol 2021 Jan 24;90:107160. Epub 2020 Nov 24.

Department of Intensive Care Unit, Shandong Provincial Hospital, Cheeloo College of Medicine, Shandong University, Jinan, Shandong, China. Electronic address:

Septic cardiomyopathy (SCM)is common in septic patients and results in cardiovascular failure. The pathogenesis of SCM is complicated, and patients with SCM have high mortality because current treatment methods are limited. The cholinergic anti-inflammatory pathway (CAP) modulates inflammatory responses through vagus nerve stimulation that leads to the release of acetylcholine (ACh), which binds to the alpha7 nicotinic acetylcholine receptor (α7nAChR). Moreover, α7nAChR activation by its agonists at the tissue level inhibits inflammatory mediators and regulates the function of immune cells in sepsis. Therefore, the α7nAChR can maintain balance of the inflammatory-immune response in sepsis. CAP has been elucidated as a critical regulator of anti-inflammation in many diseases, including rheumatoid arthritis, inflammatory boweldisease and SCM. Additionally, some clinical and preclinical trials show therapeutic potential via regulating CAP. There are excellent studies regarding the beneficial role of CAP activation, especially α7nAChR, in experimental SCM. This review aims to discuss the CAP in attenuating inflammation and the potential role of α7nAChR activation in regulating immune and reducing inflammation in SCM.
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http://dx.doi.org/10.1016/j.intimp.2020.107160DOI Listing
January 2021

Development and validation of metabolism-related gene signature in prognostic prediction of gastric cancer.

Comput Struct Biotechnol J 2020 17;18:3217-3229. Epub 2020 Oct 17.

Department of Gastric Surgery, Sun Yat-sen University Cancer Center, State Key Laboratory of Oncology in South China, Collaborative Innovation Center for Cancer Medicine, Guangzhou 510060, China.

Gastric cancer is one of the most common malignant tumours in the world. As one of the crucial hallmarks of cancer reprogramming of metabolism and the relevant researches have a promising application in the diagnosis treatment and prognostic prediction of malignant tumours. This study aims to identify a group of metabolism-related genes to construct a prediction model for the prognosis of gastric cancer. A large cohort of gastric cancer cases (1121 cases) from public database was included in our analysis and classified patients into training and testing cohorts at a ratio of 7: 3. After identifying a list of metabolism-related genes having prognostic value, we constructed a risk score based on metabolism-related genes using LASSO-COX method. According to the risk score, patients were divided into high- and low-risk groups. Our results revealed that high-risk patients had a significantly worse prognosis than low-risk patients in both the training (high-risk vs low-risk patients; five years overall survival: 37.2% vs 72.2%;  < 0.001) and testing cohorts (high-risk vs low-risk patients; five years overall survival: 42.9% vs 62.9%;  < 0.001). This observation was validated in the external validation cohort (high-risk vs. low-risk patients; five years overall survival: 30.2% vs 40.4%;  = 0.007). To reinforce the predictive ability of the model, we integrated risk score, age, adjuvant chemotherapy, and TNM stage into a nomogram. According to the result of receiver operating characteristic curves and decision curves analysis, we found that the nomogram score had a superior predictive ability than conventional factors, indicating that the risk score combined with clinicopathological features can develop a robust prediction for survival and improve the individualized clinical decision making of the patient. In conclusion, we identified a list of metabolic genes related to survival and developed a metabolism-based predictive model for gastric cancer. Through a series of bioinformatics and statistical analyses, the predictive ability of the model was confirmed.
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http://dx.doi.org/10.1016/j.csbj.2020.09.037DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7649605PMC
October 2020

Molecular characterization, expression and interaction of MAPK, MAPKK and MAPKKK genes in upland cotton.

Genomics 2021 Jan 9;113(1 Pt 2):1071-1086. Epub 2020 Nov 9.

Research Base, Zhengzhou University, State Key Laboratory of Cotton Biology, Institute of Cotton Research of Chinese Academy of Agricultural Sciences, Henan, PR China. Electronic address:

Mitogen-activated protein kinase (MAPK) signaling cascades, consisting of three types of sequentially phosphorylated kinases (MAPKKK, MAPKK, and MAPK), play vital roles in various processes including plant development and stress response. In this study, 52 GhMAPKs, 23 GhMAPKKs, and 166 GhMAPKKKs were identified in upland cotton. Chromosomal locations, gene duplication and structure, motifs, cis-regulatory elements, and protein subcellular localization were further analyzed. With the identified MAPK cascade genes in G. arboretum and G. raimondii, a syntenic diagram of three cotton species was constructed. The interactions of seven GhMAPK cascade genes were investigated. Two complete signaling modules were defined: The GhMEKK24/GhMEKK31-GhMAPKK9-GhMAPK10 and GhMEKK3/GhMEKK24/GhMEKK31-GhMAPKK16-GhMAPK10/GhMAPK11 cascades. Moreover, interaction networks and the interaction pairs were combined with their expression patterns and demonstrated that the network mediated by the MAPK signaling cascade participates in abiotic stress signaling. Our research provides a foundation for studying the molecular mechanism of the MAPK signaling pathway under abiotic stress.
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http://dx.doi.org/10.1016/j.ygeno.2020.11.004DOI Listing
January 2021

Curtailing FGF19's mitogenicity by suppressing its receptor dimerization ability.

Proc Natl Acad Sci U S A 2020 11 3;117(46):29025-29034. Epub 2020 Nov 3.

School of Pharmaceutical Science, Wenzhou Medical University, Wenzhou, 325035 Zhejiang, China;

As a physiological regulator of bile acid homeostasis, FGF19 is also a potent insulin sensitizer capable of normalizing plasma glucose concentration, improving lipid profile, ameliorating fatty liver disease, and causing weight loss in both diabetic and diet-induced obesity mice. There is therefore a major interest in developing FGF19 as a therapeutic agent for treating type 2 diabetes and cholestatic liver disease. However, the known tumorigenic risk associated with prolonged FGF19 administration is a major hurdle in realizing its clinical potential. Here, we show that nonmitogenic FGF19 variants that retain the full beneficial glucose-lowering and bile acid regulatory activities of WT FGF19 (FGF19) can be engineered by diminishing FGF19's ability to induce dimerization of its cognate FGF receptors (FGFR). As proof of principle, we generated three such variants, each with a partial defect in binding affinity to FGFR (FGF19) and its coreceptors, i.e., βklotho (FGF19) or heparan sulfate (FGF19). Pharmacological assays in WT and mice confirmed that these variants incur a dramatic loss in mitogenic activity, yet are indistinguishable from FGF19 in eliciting glycemic control and regulating bile acid synthesis. This approach provides a robust framework for the development of safer and more efficacious FGF19 analogs.
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http://dx.doi.org/10.1073/pnas.2010984117DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7682408PMC
November 2020

LncRNA SNHG11 facilitates tumor metastasis by interacting with and stabilizing HIF-1α.

Oncogene 2020 11 15;39(46):7005-7018. Epub 2020 Oct 15.

Fudan University Shanghai Cancer Center and Institutes of Biomedical Sciences, Shanghai Medical College, Fudan University, Shanghai, 200032, China.

Epigenetic alteration is one of the hallmarks of colorectal cancer (CRC). Many driver genes are regulated by DNA methylation in CRC. However, the role of DNA methylation regulating lncRNAs remain elusive. Here, we identify that SNHG11 (small nucleolar RNA host gene 11) is upregulated by promotor hypomethylation in CRC and is associated with poor prognosis in CRC patients. SNHG11 can promote CRC cell migration and metastasis under hypoxia. Interestingly, the DNA-binding motif of SNHG11 is similar to that of HIF-1α. In addition, SNHG11-associated genes are enriched with members of the HIF-1 signaling pathway in CRC. Mechanistically, SNHG11 binds to the pVHLrecognition sites on HIF-1α, thus blocking the interaction of pVHL with HIF-1α and preventing its ubiquitination and degradation. Moreover, SNHG11 upregulates the expression of HIF-1α target genes, i.e., AK4, ENO1, HK2, and Twist1. Notably, SNHG11 can bind to the HRE sites in the promoter of these genes and increase their transcription. In summary, these results identify a SNHG11/ HIF-1α axis that plays a pivotal role in tumor invasion and metastasis.
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http://dx.doi.org/10.1038/s41388-020-01512-8DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7661343PMC
November 2020

Corrigendum: The Extracts of and Its Hairy Roots Attenuate Dextran Sodium Sulfate-Induced Chronic Ulcerative Colitis in Mice by Regulating Inflammation and Lymphocyte Apoptosis.

Front Immunol 2020 11;11:2092. Epub 2020 Sep 11.

Guangdong Provincial Key Laboratory of New Chinese Medicinals Development and Research, Guangzhou University of Chinese Medicine, Guangzhou, China.

[This corrects the article DOI: 10.3389/fimmu.2017.00905.].
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http://dx.doi.org/10.3389/fimmu.2020.02092DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7518505PMC
September 2020

Electric-Field-Mediated Electron Tunneling of Supramolecular Naphthalimide Nanostructures for Biomimetic H Production.

Angew Chem Int Ed Engl 2021 01 13;60(3):1235-1243. Epub 2020 Nov 13.

State Key Laboratory of Photocatalysis on Energy and Environment, College of Chemistry, Fuzhou University, Fuzhou, 350108, P. R. China.

The design and synthesis of two semiconducting bis (4-ethynyl-bridging 1, 8-naphthalimide) bolaamphiphiles (BENI-COO and BENI-NH ) to fabricate supramolecular metal-insulator-semiconductor (MIS) nanostructures for biomimetic hydrogen evolution under visible light irradiation is presented. A H evolution rate of ca. 3.12 mmol g ⋅h and an apparent quantum efficiency (AQE) of ca. 1.63 % at 400 nm were achieved over the BENI-COO -NH -Ni MIS photosystem prepared by electrostatic self-assembly of BENI-COO with the opposite-charged DuBois-Ni catalysts. The hot electrons of photoexcited BENI-COO nanofibers were tunneled to the molecular Ni collectors across a salt bridge and an alkyl region of 2.2-2.5 nm length at a rate of 6.10×10  s , which is five times larger than the BENI-NH nanoribbons (1.17×10  s ). The electric field benefited significantly the electron tunneling dynamics and compensated the charge-separated states insufficient in the BENI-COO nanofibers.
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http://dx.doi.org/10.1002/anie.202009267DOI Listing
January 2021

Complete chemical structures of human mitochondrial tRNAs.

Nat Commun 2020 08 28;11(1):4269. Epub 2020 Aug 28.

Department of Chemistry and Biotechnology, Graduate School of Engineering, University of Tokyo, Bunkyo-ku, Tokyo, 113-8656, Japan.

Mitochondria generate most cellular energy via oxidative phosphorylation. Twenty-two species of mitochondrial (mt-)tRNAs encoded in mtDNA translate essential subunits of the respiratory chain complexes. mt-tRNAs contain post-transcriptional modifications introduced by nuclear-encoded tRNA-modifying enzymes. They are required for deciphering genetic code accurately, as well as stabilizing tRNA. Loss of tRNA modifications frequently results in severe pathological consequences. Here, we perform a comprehensive analysis of post-transcriptional modifications of all human mt-tRNAs, including 14 previously-uncharacterized species. In total, we find 18 kinds of RNA modifications at 137 positions (8.7% in 1575 nucleobases) in 22 species of human mt-tRNAs. An up-to-date list of 34 genes responsible for mt-tRNA modifications are provided. We identify two genes required for queuosine (Q) formation in mt-tRNAs. Our results provide insight into the molecular mechanisms underlying the decoding system and could help to elucidate the molecular pathogenesis of human mitochondrial diseases caused by aberrant tRNA modifications.
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http://dx.doi.org/10.1038/s41467-020-18068-6DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7455718PMC
August 2020

Role of pulmonary function and FeNO detection in early screening of patients with ACO.

Exp Ther Med 2020 Aug 17;20(2):830-837. Epub 2020 May 17.

Department of Intensive Care Unit, Shandong Provincial Hospital Affiliated to Shandong University, Jinan, Shandong 250021, P.R. China.

Measurement of fractional exhaled nitric oxide (FeNO) is a quantitative and non-invasive approach to examine airway inflammation, which is a powerful aid in diagnosing chronic disorders of airways like asthma. Diagnostic value of FeNO and relevant indices on pulmonary function in the patients with asthma and chronic obstructive pulmonary disease (COPD) was evaluated. A total of 164 patients [58 asthma, 49 COPD and 57 asthma-COPD overlap (ACO)] were randomly recruited. FeNO, pulmonary ventilation function, and bronchial diastolic function were performed. Eight indicators including FeNO, vital capacity percentage (VC%), forced vital capacity percentage (FVC%), forced expiratory volume in one second percentage (FEV1%), forced expiratory volume in one second to forced vital capacity percentage (FEV1/FVC%), maximum independent ventilation volume percentage (MVV%), the increased percentage of FEV1 after bronchial diastolic test, the increased absolute value of FEV1 after bronchial diastolic test were examined. Significant difference in VC%, FVC%, FEV1%, FEV1/FVC%, MVV%, the increased absolute value of FEV1 after bronchial diastolic test and FeNO were significantly different between patients with asthma and patients with COPD (P<0.05). There were significant differences of VC%, FVC%, FEV1%, FEV1/FVC%, MVV% and the increased percentage of FEV1 after bronchial diastolic test in cases of patients with asthma compared to ACO patients (P<0.05). There was no statistical significance on VC%, FVC%, FEV1%, FEV1/FVC%, MVV% between COPD patients and ACO patients (P>0.05). However, more importantly, the increased percentage of FEV1 after bronchial diastolic test, the increased absolute value of FEV1 after bronchial diastolic test and the alterations on FeNO were found significantly different in ACO group compared with COPD alone (P<0.05). We compared the results from pulmonary ventilation function, bronchial diastolic function examination as well as FeNO detection among 3 groups of asthma, COPD and ACO. The examination of pulmonary ventilation function and bronchial diastolic function combined with FeNO detection is helpful in the early screening of ACO.
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http://dx.doi.org/10.3892/etm.2020.8762DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7388375PMC
August 2020

Improving the Interfacial Stability between Lithium and Solid-State Electrolyte via Dipole-Structured Lithium Layer Deposited on Graphene Oxide.

Adv Sci (Weinh) 2020 Jul 18;7(13):2000237. Epub 2020 May 18.

Ningbo Institute of Materials Technology and Engineering Chinese Academy of Sciences Ningbo 315201 China.

Utilization of lithium (Li) metal anode in solid-state batteries (SSBs) with sulfide solid-state electrolyte (SSE) is hindered by the instable Li/SSE interface. A general solution to solve this problem is to place an expensive indium (In) foil between the SSE and Li, while it decreases the output voltage and thus the energy density of the battery. In this work, an alternative strategy is demonstrated to boost the cycling performances of SSB by wrapping a graphene oxide (GO) layer on the anode. According to density functional theory results, initial deposition of a thin Li layer on the defective GO sheets leads to the formation of a dipole structure, due to the electron-withdrawing ability of GO acting on Li. By incorporating GO sheets in a nanocomposite of copper-cuprous oxide-GO (Cu-CuO-GO, CCG), a composite Li anode enables a high coulombic efficiency above 99.5% over 120 cycles for an SSB using LiGePS SSE and LiCoO cathode, and the sulfide SSE is not chemically decomposed after cycling. The highest occupied molecule orbital/lowest unoccupied molecular orbital energy gap of this Li/GO dipole structure likely stretches over those of Li and sulfide SSE, enabling stabilized Li/SSE interface that can replace the expensive In layer as Li protective structure in SSBs.
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http://dx.doi.org/10.1002/advs.202000237DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7341093PMC
July 2020

Altered White Matter Structural Network in Frontal and Temporal Lobe Epilepsy: A Graph-Theoretical Study.

Front Neurol 2020 17;11:561. Epub 2020 Jun 17.

Department of Radiology, First Affiliated Hospital of Guangzhou University of Chinese Medicine, Guangzhou, China.

Temporal lobe epilepsy (TLE) and frontal lobe epilepsy (FLE) are the largest subgroup of partial epilepsy, and focal cortical dysplasias (FCDs) are highly epileptogenic brain lesions and are a frequent cause for antiepileptic drug (AED)-resistant focal epilepsies that mostly occur in the temporal and frontal lobes. We performed a graph-theoretical study based on the diffusion tensor imaging (DTI) data of patients with FLE or TLE caused by FCDs or lesions with high suspicion of FCDs and evaluated their cognitive function by the Chinese version of the Montreal Cognitive Assessment-Basic (MoCA-BC). The construction of the white matter structural network and graph-theoretical analysis was performed by Pipeline for Analysing Brain Diffusion Images (PANDA) and Graph-theoretical Network Analysis (GRETNA). We used the nonparametric analysis of covariance to compare the differences in diffusion metrics, network attributes and nodal attributes among FLE, TLE, and healthy control (HC) groups and then performed pairwise comparisons. Nonparametric Spearman partial correlation analysis was performed to analyse the correlation of network attributes with the age of onset, duration of disease, and MoCA-BC scores in patients with FLE and TLE. The results showed that the white matter structural network in patients with FLE and TLE was impaired in a more extensive set of regions than the FCD location. The similarities in white matter alterations between FLE and TLE suggested that their epileptogenic network might affect the fronto-temporal white matter tracts and thalamo-occipital connections, which might be responsible for the overlapping cognitive deficits in FLE and TLE. The white matter impairments in patients with FLE were more severe than those in patients with TLE, which might be explained by more affected nodes in the areas of DMN in patients with FLE.
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http://dx.doi.org/10.3389/fneur.2020.00561DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7311567PMC
June 2020

Flexible porphyrin doped polymer optical fibers for rapid and remote detection of trace DNT vapor.

Analyst 2020 Aug 19;145(15):5307-5313. Epub 2020 Jun 19.

College of Chemical Engineering, Fuzhou University, Fuzhou, 350116, China.

With the rapid growth of anti-terrorist activities worldwide, it becomes an emerging requirement to rapidly and accurately detect hidden explosive threats. However, the safety issue during the explosive material detection, e.g. unexpected explosion, is still an insurmountable challenge. In this study, we design and mass-produce a novel kind of flexible 5,10,15,20-tetrakis(4-aminophenyl)porphyrin doped polymer optical fiber (PPOF) for rapid and accurate detection of trace 2,4-dinitrotoluene (DNT) vapor based on the DNT induced florescence quenching mechanism. The influence of doping concentration, bending, and temperature on the sensing performance is investigated. PPOF shows immunity to bending, enabling it to work in a harsh environment. It is experimentally demonstrated that the limit of detection and response time of the proposed PPOF could reach around 120 ppb and 3 minutes, respectively, which make it much better than other techniques. Owning to its inherent advantages including low-cost, remote-control capability, and compatibility with optical communication networking, PPOF can be constructed the quasi-distributed sensing networking of explosive matters in the future, providing a new strategy for anti-terrorism.
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http://dx.doi.org/10.1039/d0an00706dDOI Listing
August 2020

Transplantable Carbonaceous Li Filtrating Membrane for Lithium Metal Protection.

ACS Appl Mater Interfaces 2020 Jul 25;12(27):30494-30502. Epub 2020 Jun 25.

Key Laboratory of Optoelectronic Chemical Materials and Devices, School of Chemical and Environmental Engineering, Jianghan University, Wuhan 430056, China.

Utilization of the lithium (Li) metal anode is seriously prevented by the undesirable side reactions with electrolyte solvents due to their mismatched energy gaps and easily lacerated SEI layer. In this work, we develop a transplantable carbonaceous membrane with a particular ability of filtrating Li ions by blocking organic solvents and use it as an independent protective component to isolate lithium metal anode from the electrolytes. This graphene-supported N-doped membrane (GNM) can separate organic carbonates of dimethyl carbonate (DMC) and diethyl carbonate (DEC) from HO-DMC/DEC mixtures by holding back the organic solvents. When this membrane is used in a Li-Cu cell, a high Li Coulombic efficiency (CE) of 98.5% is maintained in carbonate electrolyte over 400 cycles. Application of GNM in Li-O full cell provides a sustainable use of Li metal for more than 200 cycles (2000 h) by keeping its shiny metal luster. Our results demonstrate that the use of an independent component with Li filtrating ability, such as the transplantable membrane of GNM developed in this work, should be a feasible remedy to protect Li metal anode in practical Li metal batteries.
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http://dx.doi.org/10.1021/acsami.0c08433DOI Listing
July 2020

Characterization of Thermal and Electrical Transport in 6.4 nm Au Films on Polyimide Film and Fiber Substrates.

Sci Rep 2020 Jun 8;10(1):9165. Epub 2020 Jun 8.

School of Environmental and Municipal Engineering, Qingdao University of Technology, Qingdao, Shandong, 266033, P.R. China.

The surface and grain boundary scattering impact on the electrical and thermal conduction in the thin metallic films coated on organic substrates has not been studied thoroughly. In this work, we study heat and electron transport in the 6.4 nm thin Au films supported by polyimide (PI) substrate using the transient electro-thermal technique. Thermal and electrical conductivities of 6.4 nm thin Au film are much smaller than bulk value. The thermal and electrical conductivities of 6.4 nm Au film deposited on the PI fiber are reduced by 59.3% and 76.8% in the comparison with the value of bulk Au. For PI film, the reduction of thermal and electrical conductivities is 47.9% and 46.3%. Lorenz numbers of 6.4 nm Au film supported by PI fiber and PI film are 4.51 × 10 WΩK and 2.12 × 10 WΩK, respectively. The thermal conductivities of PI fiber and PI film are 0.87 WmK and 0.44 WmK. The results reveal that PI is a suitable substrate material in the flexible electronic devices field.
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http://dx.doi.org/10.1038/s41598-020-66174-8DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7280525PMC
June 2020

Development and Pilot Testing a Self-Reported Pediatric PROMIS App for Young Children Aged 5-7 Years.

J Pediatr Nurs 2020 Jul - Aug;53:74-83. Epub 2020 May 28.

School of Nursing, Navy Medical University, Shanghai, China.

Purpose: The aims of this study are threefold. Firstly, Using the state of science PROMIS (Patient-Reported Outcomes Measurement Information System) methods to develop a smartphone application to monitor the emotional distress for young children aged 5-7 years old; Secondly, to test the usability of this application; and thirdly, to determine the level of agreement between reports by parents and young children's self-report.

Design And Methods: A multidisciplinary research team, made up of senior pediatric nurses and doctors, software engineers' team, and pediatric health researchers worked together to develop this application. Three phases of stakeholders and user studies were conducted. Phase 1 focused on prototype development; Phase 2 involved cognitive interview and usability testing; Phases 3 focused on the pilot testing of this application.

Results: We included the original parent proxy reporting version of Patient Reported Outcome Measurement Information System-emotional distress in the application, as well as self-reporting animated version for young children. After many rounds of modification, all participants felt that this application was easy to use and the animated items were easy to understand for young children aged 5-7 years. Correlations between parents-children reports are significant and moderate, parents underestimated child depression, and overestimated child anger and anxiety compared to child self-report.

Conclusions: This smartphone application and its Web-based administration portal demonstrate good usability and are well accepted by young children aged 5-7 years, which can be used to promote young children's participation when reporting or assessing symptoms of young pediatric patients.

Practice Implications: Parent reports cannot be substituted for child reports and evaluations of pediatric patients' perspectives regarding treatment outcomes should be included in pediatric clinic. This animated application can be used as a smart measurement to investigate the symptoms for young children aged 5-7 years, so as to amplify young children's voice in clinical care.
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http://dx.doi.org/10.1016/j.pedn.2020.04.003DOI Listing
April 2021

Dental expenditure, progressivity and horizontal inequality in Chinese adults: based on the 4th National Oral Health Epidemiology Survey.

BMC Oral Health 2020 05 11;20(1):137. Epub 2020 May 11.

Guangdong Provincial Key Laboratory of Stomatology, Guangzhou, China.

Background: The financial burden of oral diseases is a growing concern as the medical expenses rise worldwide. The aim of this study was to investigate the dental expenditure, analyze its progressivity and horizontal inequality under the general health finance and insurance system, and identify the key social determinants of the inequality for Chinese adults.

Methods: A secondary analysis used the data of 13,464 adults from the 4th National Oral Health Epidemiological Survey (NOHES) in China was undertaken. The dental expenditure was collected and divided into out-of-pocket and health insurance payments. Horizontal inequality index and Kakwani index were used to analyze the horizontal inequality and progressivity, respectively. The decomposition model of the concentration index was set up to explore the associated socioeconomic determinants.

Results: The results showed that a mean dental expenditure per capita of Chinese adults was $20.55 (95% Confidence Interval-CI: 18.83,22.26). Among those who actually used dental service, the cost was $100.95 (95%CI: 93.22,108.68). Over 90% of dental spending was due to out-of-pocket expenses. For self-reported oral health, the horizontal inequality index was - 0.1391 and for the decayed tooth (DT), it was - 0.2252. For out-of-pocket payment, the Kakwani index was - 0.3154 and for health insurance payment it was - 0.1598. Income, residential location, educational attainment, oral hygiene practice, self-reported oral health, age difference were the main contributors to the inequality of dental expenditure.

Conclusion: Dental expenditure for Chinese adults was at a lower level due to underutilization. The ratio of payments of dental expenditure and utilization was disproportional, whether it was out-of-pocket or insurance payment. Individuals who were more in need of oral care showed less demand for service or not required service in time. For future policy making on oral health, it is worth the effort to further promote the awareness of the importance of oral health and utilization of dental service.
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http://dx.doi.org/10.1186/s12903-020-01128-0DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7216389PMC
May 2020

Wait-and-See Treatment Strategy Could be Considered for Lung Adenocarcinoma with Special Pleural Dissemination Lesions, and Low Genomic Instability Correlates with Better Survival.

Ann Surg Oncol 2020 Oct 1;27(10):3808-3818. Epub 2020 Apr 1.

Guangdong Lung Cancer Institute, Guangdong Provincial Key Laboratory of Translational Medicine in Lung Cancer, Guangdong Provincial People's Hospital and Guangdong Academy of Medical Sciences, Guangzhou, China.

Background: This study aimed to evaluate the feasibility of a wait-and-see strategy for non-small cell lung cancer (NSCLC) patients with special pleural dissemination lesions (r-pM1a and s-pM1a). Furthermore, the study characterized genomic alternations about disease progression.

Methods: For this study, 131 NSCLC patients with a diagnosis of pM1a were retrospectively selected. Survival differences were evaluated among patients treated with three different initial postoperative treatments: chemotherapy, targeted therapy, and wait-and-see strategy. Whole-exome sequencing (WES) was performed on primary and metastatic tumors of 10 patients with dramatic progression and 13 patients with gradual progression.

Results: The wait-and-see group showed better progression-free survival (PFS) than the chemotherapy group (p < 0.001) but PFS similar to that of targeted group (p = 0.984). This pattern persisted in epidermal growth factor receptor (EGFR)-positive patients. For patients with EGFR-negative/unknown status, PFS was longer in the wait-and-see group than in the two treatment groups. Furthermore, better overall survival (OS) was observed for the patients who received chemotherapy or targeted therapy after the wait-and-see strategy than for those who received chemotherapy or targeted therapy immediately. Lymph node status was an independent prognostic factor for PFS and OS. Finally, WES analysis showed that a high genomic instability index (GIS) and chromosome 18q loss were more common in metastatic tumors, and low GIS was significantly associated with better PFS (p = 0.016).

Conclusions: The wait-and-see strategy could be considered for special pM1a patients without lymph nodes metastasis, and patients with a low GIS may be suitable for this strategy.
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http://dx.doi.org/10.1245/s10434-020-08400-1DOI Listing
October 2020

Current Status of Septic Cardiomyopathy: Basic Science and Clinical Progress.

Front Pharmacol 2020 3;11:210. Epub 2020 Mar 3.

Department of Intensive Care Unit, Shandong Provincial Hospital Affiliated to Shandong University, Jinan, China.

Septic cardiomyopathy (SCM) is a complication that is sepsis-associated cardiovascular failure. In the last few decades, there is progress in diagnosis and treatment despite the lack of consistent diagnostic criteria. According to current studies, several hypotheses about pathogenic mechanisms have been revealed to elucidate the pathophysiological characteristics of SCM. The objective of this manuscript is to review literature from the past 5 years to provide an overview of current knowledge on pathogenesis, diagnosis and treatment in SCM.
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http://dx.doi.org/10.3389/fphar.2020.00210DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7062914PMC
March 2020

Epidemiology of pediatric eye injuries requiring hospitalization in rural areas of Wenzhou and Changsha, China: a 10-year retrospective study.

BMC Ophthalmol 2020 Mar 14;20(1):100. Epub 2020 Mar 14.

Ophthalmology Department, Xiangya Hospital, Central South University, Changsha, Hunan, China.

Background: The aim of this study was to review the demographic and characteristic distribution data of serious rural pediatric eye injuries in Wenzhou and Changsha, located in Zhejiang Province in East China and Hunan Province in Central China.

Methods: This retrospective study included hospitalized pediatric patients aged < 18 years with eye injuries at the Eye Hospital of Wenzhou Medical University and Xiangya Hospital of Central South University from January 2008 to December 2017. Demographic data, injury types, injury causes, and initial and final visual acuity (VA) were recorded and analyzed. The ocular trauma score (OTS) was calculated to assess the severity of injury and evaluate the prognosis. All patient data were obtained from the medical record systems.

Results: In total, 1125 children were hospitalized during the 10-year period; 830 (73.8%) were males and 295 (26.2%) were females. The majority of the patients were aged 3 to 8 years (57.4%, n = 646). Among mechanical injuries (n = 1007), penetrating injury was the most common (68.4%, n = 689), followed by contusion (17.2%, n = 173) and rupture (8.1%, n = 82). Overall, the top three injury causes were sharp objects (n = 544, 48.4%), blunt objects (n = 209, 18.6%) and fireworks (n = 121, 10.8%). In Wenzhou, eye injuries occurred mostly in summer (n = 136, 29.1%), and sharp object-related eye injuries accounted for the highest proportion (n = 98, 72.1%). In Changsha, eye injuries occurred mostly in winter (n = 272, 41.3%), and firecracker- and fireworks-associated eye injury accounted for the highest proportion (n = 73, 26.8%). The final VA was positively correlated with the initial VA (r = 0.641, P < 0.001) and the OTS (r = 0.582, P < 0.001).

Conclusion: The age range of the susceptible pediatric population from rural areas was 3-8 years. Most eye injuries were penetrating, and the main cause of injury was a sharp object. Notably, the differences in the characteristics of eye injuries in the two areas were related to regional features.
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http://dx.doi.org/10.1186/s12886-020-01363-7DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7071590PMC
March 2020

Inflammation-Induced Long Intergenic Noncoding RNA (LINC00665) Increases Malignancy Through Activating the Double-Stranded RNA-Activated Protein Kinase/Nuclear Factor Kappa B Pathway in Hepatocellular Carcinoma.

Hepatology 2020 11 19;72(5):1666-1681. Epub 2020 Oct 19.

Fudan University Shanghai Cancer Center and Institutes of Biomedical Sciences, Department of Oncology, Shanghai Medical College, Fudan University, Shanghai, China.

Background And Aims: The nuclear factor kappa B (NF-κB) signaling pathway is important for linking inflammation and tumorigenesis. Here, we characterized an NF-κB signaling activation-induced long intergenic noncoding (LINC) RNA in hepatocellular carcinoma (HCC), LINC00665, that contributes to the enhanced cell proliferation of HCC cells both in vitro and in vivo.

Approach And Results: LINC00665 physically interacts with the double-stranded RNA (dsRNA)-activated protein kinase (PKR), enhances its activation, and maintains its protein stability by blocking ubiquitin/proteasome-dependent degradation, resulting in a positive feedback regulation of NF-κB signaling in HCC cells. Notably, patients with HCC and higher LINC00665 have poorer outcomes in the clinic.

Conclusions: Our findings indicate that LINC00665 is involved in the NF-κB signaling activation in HCC cells and that the inflammatory LINC00665/PKR/NF-κB loop plays important oncogenic roles in hepatic cancer progression and may be a potential therapeutic target.
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http://dx.doi.org/10.1002/hep.31195DOI Listing
November 2020

Hypoxia induced LUCAT1/PTBP1 axis modulates cancer cell viability and chemotherapy response.

Mol Cancer 2020 01 21;19(1):11. Epub 2020 Jan 21.

Fudan University Shanghai Cancer Center and Institutes of Biomedical Sciences, Shanghai Medical College, Fudan University, Shanghai, 200032, China.

Background: Hypoxic tumors are refractory to DNA damage drugs. However, the underlying mechanism has yet to be elucidated. We aimed to identify lncRNAs that upregulated under hypoxia and their effects on colorectal cancer (CRC).

Methods: CRC cells were treated with 1% O to identify lncRNAs that upregulated under hypoxia. We integrated these lncRNAs with RNA-seq of 4 paired CRC tissues and TCGA data to get candidate lncRNAs. Multiple in vitro and in vivo assays were used to explore the role of LUCAT1 in CRC.

Results: We identified a hypoxia-induced lncRNA LUCAT1 that facilitated the growth of CRC cells and contributed to drug resistance of CRC cells both in vitro and in vivo. Mechanically, LUCAT1 interacts with polypyrimidine tract binding protein 1 (PTBP1) in CRC cells, facilitates the association of a set of DNA damage related genes with PTBP1, thus resulting in altered alternative splicing of these genes. Moreover, ectopic expression of PTBP1 in CRC cells with knockdown of LUCAT1 abrogated the effects induced by LUCAT1 knockdown. Chemotherapeutics drug combined with LUCAT1 knockdown via antisense oligonucleotides (ASO) would get a better outcome in vivo, compared with group treated with chemotherapeutic drug only. Notably, LUCAT1 is upregulated in CRC tissues, compared to adjacent normal tissues; and CRC patients with higher LUCAT1 have a worse prognosis and poorly responded to chemotherapy in the clinic.

Conclusions: Our data suggested CRC cells utilizes LUCAT1 to develop resistance to DNA damage drugs, and disrupting the LUCAT1/PTBP1 axis might be a promising therapeutic strategy for refractory hypoxic tumors.
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http://dx.doi.org/10.1186/s12943-019-1122-zDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6971890PMC
January 2020

Paracrine Fibroblast Growth Factor 1 Functions as Potent Therapeutic Agent for Intrahepatic Cholestasis by Downregulating Synthesis of Bile Acid.

Front Pharmacol 2019 20;10:1515. Epub 2019 Dec 20.

School of Pharmacy, Wenzhou Medical University, Wenzhou, China.

Endocrine fibroblast growth factor (FGF) 19 has been shown to be capable of maintaining bile acid (BA) homeostasis and thus hold promise to be a potential therapeutic agent for cholestasis liver disease. However, whether paracrine FGFs possess this BA regulatory activity remains to be determined. In our study, we identified that paracrine fibroblast growth factor 1 (FGF1) was selectively downregulated in the liver of alpha naphthylisothiocyanate (ANIT)-induced intrahepatic cholestasis mice, suggesting a pathological relevance of this paracrine FGF with abnormal BA metabolism. Therefore, we evaluated the effects of engineered FGF1 mutant - FGF1 on the metabolism of hepatic BA and found that this protein showed a more potent inhibitory effect of BA biosynthesis than FGF19 without any hepatic mitogenic activity. Moreover, the chronic administration of FGF1 protected liver against ANIT-induced injury by reducing hepatic BA accumulation. Taken together, these data suggest that FGF1 may function as a potent therapeutic agent for intrahepatic cholestasis liver disease.
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http://dx.doi.org/10.3389/fphar.2019.01515DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6933012PMC
December 2019

Molecular Engineering of Fully Conjugated sp Carbon-Linked Polymers for High-Efficiency Photocatalytic Hydrogen Evolution.

ChemSusChem 2020 Feb 21;13(4):672-676. Epub 2020 Jan 21.

State Key Lab of Photocatalysis on Energy and Environment, College of Chemistry, Fuzhou University, Fuzhou, 350108, P.R. China.

The diverse nature of organic precursors offers a versatile platform for precisely tailoring the electronic properties of semiconducting polymers. In this study, three fully conjugated sp carbon-linked polymers have been designed and synthesized for photocatalytic hydrogen evolution under visible-light illumination, by copolymerizing different C -symmetric aromatic aldehydes as knots with the 1,4-phenylene diacetonitrile (PDAN) linker through a C=C condensation reaction. The hydrogen evolution (HER) is achieved at a maximum rate of 30.2 mmol g  h over a polymer based on 2,4,6-triphenyl-1,3,5-triazine units linked by cyano-substituted phenylene, with an apparent quantum yield (AQY) of 7.20 % at 420 nm. Increasing the degree of conjugation and planarity not only extends visible-light absorption, but also stabilizes the fully conjugated sp -carbon-linked donor-acceptor (D-A) polymer. Incorporating additional electron-withdrawing triazine units into the D-A polymer to form multiple electron donors and acceptors can greatly promote exciton separation and charge transfer, thus significantly enhancing the photocatalytic activity.
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http://dx.doi.org/10.1002/cssc.201903334DOI Listing
February 2020

An LTR Retrotransposon-Derived Long Noncoding RNA lncMER52A Promotes Hepatocellular Carcinoma Progression by Binding p120-Catenin.

Cancer Res 2020 03 24;80(5):976-987. Epub 2019 Dec 24.

Fudan University Shanghai Cancer Center, Key Laboratory of Medical Epigenetics and Metabolism, Institutes of Biomedical Sciences, Fudan University, Shanghai, China.

Long terminal repeat (LTR) retrotransposons are a major class of transposable elements, accounting for 8.67% of the human genome. LTRs can serve as regulatory sequences and drive transcription of tissue or cancer-specific transcripts. However, the role of these LTR-activated transcripts, especially long non-coding RNAs (lncRNA), in cancer development remains largely unexplored. Here, we identified a novel lncRNA derived from MER52A retrotransposons (lncMER52A) that was exclusively expressed in hepatocellular carcinoma (HCC). HCC patients with higher lncMER52A had advanced TNM stage, less differentiated tumors, and shorter overall survival. LncMER52A promoted invasion and metastasis of HCC cells and . Mechanistically, lncMER52A stabilized p120-catenin and triggered the activation of Rho GTPase downstream of p120-catenin. Furthermore, we found that chromatin accessibility was crucial for the expression of lncMER52A. In addition, YY1 transcription factor bound to the cryptic MER52A LTR promoter and drove lncMER52A transcription in HCC. In conclusion, we identified an LTR-activated lncMER52A, which promoted the progression of HCC cells via stabilizing p120-catenin and activating p120-ctn/Rac1/Cdc42 axis. LncMER52A could serve as biomarker and therapeutic target for patients with HCC. SIGNIFICANCE: A novel long noncoding RNA lncMER52 modulates cell migration and invasion via posttranslational control of p120-catenin protein stability. GRAPHICAL ABSTRACT: http://cancerres.aacrjournals.org/content/canres/80/5/976/F1.large.jpg.
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http://dx.doi.org/10.1158/0008-5472.CAN-19-2115DOI Listing
March 2020