Publications by authors named "Huan Cui"

26 Publications

  • Page 1 of 1

The Altered Anatomical Distribution of ACE2 in the Brain With Alzheimer's Disease Pathology.

Front Cell Dev Biol 2021 25;9:684874. Epub 2021 Jun 25.

Department of Human Anatomy, Histology, and Embryology, Neuroscience Center, School of Basic Medicine, Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences, Peking Union Medical College, Beijing, China.

The whole world is suffering from the coronavirus disease 2019 (COVID-19) pandemic, induced by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) through angiotensin-converting enzyme 2 (ACE2). Neurological manifestations in COVID-19 patients suggested the invasion of SARS-CoV-2 into the central nervous system. The present study mapped the expression level of ACE2 in 12 brain regions through immunohistochemistry and detected ACE2 in endothelial cells and non-vascular cells. The comparison among brain regions found that pons, visual cortex, and amygdala presented a relatively high level of ACE2. In addition, this study demonstrates that the protein level of ACE2 was downregulated in the basal nucleus, hippocampus and entorhinal cortex, middle frontal gyrus, visual cortex, and amygdala of the brain with Alzheimer's disease (AD) pathology. Collectively, our results suggested that ACE2 was expressed discriminatorily at different human brain regions, which was downregulated in the brain with AD pathology. This may contribute to a comprehensive understanding of the neurological symptoms caused by SARS-CoV-2 and provide clues for further research on the relationship between COVID-19 and AD.
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http://dx.doi.org/10.3389/fcell.2021.684874DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8267059PMC
June 2021

Risk of Environmental Exposure to H7N9 Influenza Virus Airborne and Surface Routes in a Live Poultry Market in Hebei, China.

Front Cell Infect Microbiol 2021 7;11:688007. Epub 2021 Jun 7.

College of Veterinary Medicine, Hebei Agricultural University, Baoding, China.

Environmental transmission of viruses to humans has become an early warning for potential epidemic outbreaks, such as SARS-CoV-2 and influenza virus outbreaks. Recently, an H7N9 virus, A/environment/Hebei/621/2019 (H7N9), was isolated by environmental swabs from a live poultry market in Hebei, China. We found that this isolate could be transmitted by direct contact and aerosol in mammals. More importantly, after 5 passages in mice, the virus acquired two adaptive mutations, PB1-H115Q and B2-E627K, exhibiting increased virulence and aerosol transmissibility. These results suggest that this H7N9 virus might potentially be transmitted between humans through environmental or airborne routes.
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http://dx.doi.org/10.3389/fcimb.2021.688007DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8216215PMC
June 2021

Lack of Spinal Neuropeptide Y Is Involved in Mechanical Itch in Aged Mice.

Front Aging Neurosci 2021 28;13:654761. Epub 2021 May 28.

Department of Anesthesiology, Peking Union Medical College Hospital, Chinese Academy of Medical Sciences, Peking Union Medical College, Beijing, China.

Neuropeptide Y (NPY) signaling plays an essential role in gating the pruritic afferent information in the spinal cord. Recent studies revealed that the aging process down-regulated the expression of NPY in the central nervous system. We propose that the lack of spinal NPY may be involved in certain types of pruritus in the elderly population. This study was designed to investigate the role of NPY in aging-induced itch using the senile mouse model. The expression of NPY in the spinal dorsal horn was compared between young (2 months old) and aged (24 months old) mice. Western blotting and immunohistochemistry showed that the expression of NPY was significantly reduced in the spinal dorsal horn in aged mice. In addition, a neuronal maker of apoptosis, TUNEL, was detected in the NPY positive neurons only in the aged spinal cord. Behavioral assay indicated that light mechanical stimulus evoked significantly more scratching in the aged than in the young mice, whereas chemical-evoked itch and pain-related behaviors were not altered. Intrathecal injection of either NPY or LP-NPY, a NPY receptor 1 (NPY1R) agonist, significantly alleviated the mechanically evoked itch in aged mice without altering the responses to chemical pruritogens. Our study suggested that downregulation of spinal NPY in the aged mice might play a role in the higher incidence of the mechanically evoked itch than that in the young mice. Therapies targeting the NPY system might serve as a potential strategy for alleviating the pruritic symptoms among the elderly population.
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http://dx.doi.org/10.3389/fnagi.2021.654761DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8192807PMC
May 2021

Curved retina-like camera array imaging system with adjustable super-resolution fovea.

Appl Opt 2021 Feb;60(6):1535-1543

To achieve non-uniform imaging with a large field of view and high efficiency as well as to obtain an adjustable fovea with super-resolution, we proposed a curved retina-like camera array imaging system (CRCS), which is built by an eight-camera array distributed non-uniformly on a curved surface and a camera coaxial with Risley prisms located in the center. By the non-uniform imaging, the field of view of the developed prototype is 150×40 with a reduction of data redundancy by 87.62%. Besides, the experimental results show that CRCS can obtain clear and sharp images of farther targets of interest around the fovea field of view with a constant focal length.
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http://dx.doi.org/10.1364/AO.417466DOI Listing
February 2021

Suppression of TLR4-MyD88 signaling pathway attenuated chronic mechanical pain in a rat model of endometriosis.

J Neuroinflammation 2021 Mar 5;18(1):65. Epub 2021 Mar 5.

Department of Human Anatomy, Histology and Embryology, Institute of Basic Medical Sciences, Neuroscience Center, Chinese Academy of Medical Sciences, School of Basic Medicine, Peking Union Medical College, Beijing, China.

Background: As a classic innate immunity pathway, Toll-like receptor 4 (TLR4) signaling has been intensively investigated for its function of pathogen recognition. The receptor is located not only on immune cells but also on sensory neurons and spinal glia. Recent studies revealed the involvement of neuronal TLR4 in different types of pain. However, the specific role of TLR4 signaling in the pain symptom of endometriosis (EM) remains obscure.

Methods: The rat endometriosis model was established by transplanting uterine horn tissue into gastrocnemius. Western blotting and/or immunofluorescent staining were applied to detect high mobility group box 1 (HMGB1), TLR4, myeloid differentiation factor-88 adaptor protein (MyD88), and nuclear factor kappa-B-p65 (NF-κB-p65) expression, as well as the activation of astrocyte and microglia. The antagonist of TLR4 (LPS-RS-Ultra, LRU) and MyD88 homodimerization inhibitory peptide (MIP) were intrathecally administrated to assess the behavioral effects of blocking TLR4 signaling on endometriosis-related pain.

Results: Mechanical hyperalgesia was observed at the graft site, while HMGB1 was upregulated in the implanted uterine tissue, dorsal root ganglion (DRG), and spinal dorsal horn (SDH). Compared with sham group, upregulated TLR4, MyD88, and phosphorylated NF-κB-p65 were detected in the DRG and SDH in EM rats. The activation of astrocytes and microglia in the SDH was also confirmed in EM rats. Intrathecal application of LRU and MIP alleviated mechanical pain on the graft site of EM rats, with decreased phosphorylation of NF-κB-p65 in the DRG and reduced activation of glia in the SDH.

Conclusions: HMGB1-TLR4-MyD88 signaling pathway in the DRG and SDH may involve in endometriosis-related hyperpathia. Blockade of TLR4 and MyD88 might serve as a potential treatment for pain in endometriosis.
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http://dx.doi.org/10.1186/s12974-020-02066-yDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7934423PMC
March 2021

Pathogenicity and transmissibility assessment of two strains of human influenza virus isolated in China in 2018.

J Int Med Res 2021 Jan;49(1):300060520982832

College of Veterinary Medicine, Hebei Agricultural University, Baoding, Hebei, China.

Objective: Influenza season occurs every year in China, but its presentation was unusual in the period from December 2017 to early 2018. During this period, influenza activity was increasing across the country and was much greater than during the same period in previous years, with great harm to people's health.

Methods: In this study, we isolated two human influenza virus strains-A/Hebei/F076/2018(H1N1) and B/Hebei/16275B/2018-from patients with severe influenza in Hebei, China, during the flu season in January 2018, and explored their genetic characteristics, pathogenicity, and transmissibility.

Results: A/Hebei/F076/2018(H1N1) belongs to the human-like H1N1 influenza virus lineage, whereas B/Hebei/16275B/2018 belongs to the Victoria lineage and is closely related to the World Health Organization reference strain B/Brisbane/60/2008. Pathogenicity tests revealed that A/Hebei/F076/2018(H1N1) replicated much more strongly in mice, with mice exhibiting 40% mortality, whereas B/Hebei/16275B/2018 was not lethal. Both viruses could be transmitted through direct contact and by the aerosol route between guinea pigs, but the H1N1 strain exhibited higher airborne transmissibility.

Conclusions: These results may contribute to the monitoring of influenza mutation and the prevention of an influenza outbreak.
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http://dx.doi.org/10.1177/0300060520982832DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7829534PMC
January 2021

A persistently low level of atmospheric oxygen in Earth's middle age.

Nat Commun 2021 01 13;12(1):351. Epub 2021 Jan 13.

Earth and Planets Laboratory, Carnegie Institution for Science, Washington, DC, 20015, USA.

Resolving how Earth surface redox conditions evolved through the Proterozoic Eon is fundamental to understanding how biogeochemical cycles have changed through time. The redox sensitivity of cerium relative to other rare earth elements and its uptake in carbonate minerals make the Ce anomaly (Ce/Ce*) a particularly useful proxy for capturing redox conditions in the local marine environment. Here, we report Ce/Ce* data in marine carbonate rocks through 3.5 billion years of Earth's history, focusing in particular on the mid-Proterozoic Eon (i.e., 1.8 - 0.8 Ga). To better understand the role of atmospheric oxygenation, we use Ce/Ce* data to estimate the partial pressure of atmospheric oxygen (pO) through this time. Our thermodynamics-based modeling supports a major rise in atmospheric oxygen level in the aftermath of the Great Oxidation Event (~ 2.4 Ga), followed by invariant pO of about 1% of present atmospheric level through most of the Proterozoic Eon (2.4 to 0.65 Ga).
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http://dx.doi.org/10.1038/s41467-020-20484-7DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7806885PMC
January 2021

A Novel Approach of Parallel Retina-Like Computational Ghost Imaging.

Sensors (Basel) 2020 Dec 11;20(24). Epub 2020 Dec 11.

School of Optics and Photonics, Beijing Institute of Technology, Key Laboratory of Biomimetic Robots and Systems, Ministry of Education, Beijing 100081, China.

Computational ghost imaging (CGI), with the advantages of wide spectrum, low cost, and robustness to light scattering, has been widely used in many applications. The key issue is long time correlations for acceptable imaging quality. To overcome the issue, we propose parallel retina-like computational ghost imaging (PRGI) method to improve the performance of CGI. In the PRGI scheme, sampling and reconstruction are carried out by using the patterns which are divided into blocks from designed retina-like patterns. Then, the reconstructed image of each block is stitched into the entire image corresponding to the object. The simulations demonstrate that the proposed PRGI method can obtain a sharper image while greatly reducing the time cost than CGI based on compressive sensing (CSGI), parallel architecture (PGI), and retina-like structure (RGI), thereby improving the performance of CGI. The proposed method with reasonable structure design and variable selection may lead to improve performance for similar imaging methods and provide a novel technique for real-time imaging applications.
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http://dx.doi.org/10.3390/s20247093DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7763361PMC
December 2020

Fcγ Receptor I-Coupled Signaling in Peripheral Nociceptors Mediates Joint Pain in a Rat Model of Rheumatoid Arthritis.

Arthritis Rheumatol 2020 10 6;72(10):1668-1678. Epub 2020 Sep 6.

Institute of Basic Medical Sciences, Neuroscience Center, Chinese Academy of Medical Sciences and Joint Laboratory of Anesthesia and Pain, Peking Union Medical College, Beijing, China.

Objective: Rheumatoid arthritis (RA) is often accompanied by joint pain and inflammation. Previous studies have demonstrated that functional Fcγ receptor I (FcγRI) is expressed in dorsal root ganglion (DRG) neurons and might contribute to pain in rodent models of antigen-induced arthritis (AIA). This study was undertaken to elucidate the roles of nociceptive neuronal FcγRI-coupled signaling in the development of joint pain in AIA.

Methods: RNA sequencing was used to investigate the transcriptome profile changes in the DRG in a rat model of AIA. A primary sensory neuron-specific Fcgr1a conditional-knockout (CKO) rat was established by crossing rats carrying a loxP-flanked Fcgr1a with a Pirt-specific Cre line. Behavioral, morphologic, and molecular studies were conducted to evaluate the differences between wild-type (WT) and CKO rats after AIA.

Results: We first showed that AIA induced a transcriptome profile change in the DRG, involving a number of key proteins downstream of the FcγRI-related signaling pathway. Compared to the WT rats, both the IgG immune complex-induced acute pain and AIA-induced pain were alleviated in CKO rats. Moreover, the AIA-induced activation of FcγRI-related signaling in DRGs was significantly reduced in CKO rats. In addition, CKO rats showed attenuated joint swelling after AIA.

Conclusion: These results indicate that activation of FcγRI-coupled signaling in DRG neurons plays an important role in the development of joint pain in AIA. Our findings may provide novel insights into the interactions between the peripheral nervous system and the immune system in pathologic conditions and might suggest potential biotargets for the treatment of pain in RA.
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http://dx.doi.org/10.1002/art.41386DOI Listing
October 2020

Self-assembling hydrogel loaded with 5-FU PLGA microspheres as a novel vitreous substitute for proliferative vitreoretinopathy.

J Biomed Mater Res A 2020 12 3;108(12):2435-2446. Epub 2020 Jul 3.

Department of Ophthalmology, Zhujiang Hospital, Southern Medical University, Guangzhou, China.

The vitreous substitute for proliferative vitreoretinopathy (PVR) surgery remains an unmet clinical need in ophthalmology. In our study, we developed an in situ formed hydrogel by crosslinking polyvinyl alcohol (PVA) and chitosan as a potential vitreous substitute. 5-fluorouracil (5-FU) Poly (lactic-co-glycolic acid) (PLGA) microspheres were developed and loaded onto the PVA/chitosan hydrogels to treat PVR. In vitro, PVA/chitosan hydrogels at four concentrations were subjected to morphological, physical, rheological analyses, and cytotoxicity was evaluated together with the characterization of 5-FU PLGA microspheres. In vivo, pharmacologically induce PVR rabbits were performed a vitrectomy. In the PVA group, 3% PVA/chitosan hydrogel was injected into the vitreous cavity. In the PVA/MS group, 3% PVA/chitosan hydrogel and 5-FU PLGA microspheres were injected. In the Control group, phosphate-buffered saline was injected. Therapeutic efficacy was evaluated with postoperative examinations and histological analyses. This study demonstrated that the 3% PVA/chitosan hydrogel showed properties similar to those of the human vitreous and could be a novel in situ crosslinked vitreous substitute for PVR. Loading 5-FU PLGA microspheres onto this hydrogel may represent an effective strategy to improve the prognosis of PVR.
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http://dx.doi.org/10.1002/jbm.a.36995DOI Listing
December 2020

Pain: A potential new label of COVID-19.

Brain Behav Immun 2020 07 7;87:159-160. Epub 2020 May 7.

Department of Human Anatomy, Histology and Embryology, Institute of Basic Medical Sciences, Neuroscience Center, Chinese Academy of Medical Sciences, School of Basic Medicine, Peking Union Medical College, Beijing 100005, China. Electronic address:

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http://dx.doi.org/10.1016/j.bbi.2020.05.025DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7204693PMC
July 2020

Identification of cellular microRNA miR-188-3p with broad-spectrum anti-influenza A virus activity.

Virol J 2020 01 30;17(1):12. Epub 2020 Jan 30.

Institute of Military Veterinary, Academy of Military Medical Sciences, 666 West Liuying Road, Changchun, 130122, Jilin, China.

Background: Influenza A virus (IAV) continues to pose serious threats to public health. The current prophylaxis and therapeutic interventions for IAV requires frequent changes due to the continuous antigenic drift and antigenic shift of IAV. Emerging evidence indicates that the host microRNAs (miRNAs) play critical roles in intricate host-pathogen interaction networks. Cellular miRNAs may directly target virus to inhibit its infection and be developed as potential anti-virus drugs.

Methods: In this study, we established a broad-spectrum anti-IAV miRNA screening method using miRanda software. The screened miRNAs were further verified by luciferase assay, viral protein expression assay and virus replication assay.

Results: Five cellular miRNAs (miR-188-3p, miR-345-5p, miR-3183, miR-15-3p and miR-769-3p), targeting 99.96, 95.31, 92.9, 94.58 and 97.24% of human IAV strains recorded in NCBI, respectively, were chosen for further experimental verification. Finally, we found that miR-188-3p downregulated PB2 expression at both mRNA and protein levels by directly targeted the predicted sites on PB2 and effectively inhibited the replication of IAV (H1N1, H5N6 and H7N9) in A549 cells.

Conclusions: This is the first report screening cellular miRNAs that broad-spectrum inhibiting IAV infection. These findings suggested that cellular miR-188-3p could be used for RNAi-mediated anti-IAV therapeutic strategies.
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http://dx.doi.org/10.1186/s12985-020-1283-9DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6993346PMC
January 2020

Sedimentology and chemostratigraphy of the terminal Ediacaran Dengying Formation at the Gaojiashan section, South China.

Geol Mag 2019 Jun;n/a

Department of Geology, University of Maryland, College Park, MD 20742, USA.

The late Ediacaran Dengying Formation (ca. 551.1-538.8 Ma) in South China is one of two successions where Ediacara-type macrofossils are preserved in carbonate facies along with skeletal fossils and bilaterian animal traces. Given the remarkable thickness of carbonate-bearing strata deposited in less than 12.3 million years, the Dengying Formation holds the potential for a relatively continuous chemostratigraphic profile for the terminal Ediacaran stage. In this study, a detailed sedimentological and chemostratigraphic (δC, δO, δC, δS, and Sr/Sr) investigation was conducted on the Dengying Formation at the Gaojiashan section, Ningqiang County of the southern Shaanxi Province, South China. Sedimentological results reveal an overall shallow marine depositional environment. Carbonate breccia, void-filling botryoidal precipitates, and aragonite crystal fans are common in the Algal Dolomite Member of the Dengying Formation, suggesting that peritidal facies were repeatedly karstified. The timing of karstification was likely early, probably soon after the deposition of the dolomite sediments. The presence of authigenic aragonite cements suggests high alkalinity in the terminal Ediacaran ocean. Geochemical analysis of micro-drilled samples shows that distinct compositions are registered in different carbonate phases, which should be considered when constructing chemostratigraphic profiles representative of true temporal variations in seawater chemistry. Integrated chemostratigraphic data suggest enhanced burial of organic carbon and pyrite, and the occurrence of extensive marine anoxia (at least in the Gaojiashan Member). Rapid basinal subsidence and carbonate accumulation during a time of elevated seawater alkalinity and increased rates of pyrite burial may have facilitated the evolutionary innovation of early biomineralizing metazoans.
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http://dx.doi.org/10.1017/S0016756819000293DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6800678PMC
June 2019

Adjacent intact nociceptive neurons drive the acute outburst of pain following peripheral axotomy.

Sci Rep 2019 05 21;9(1):7651. Epub 2019 May 21.

Institute of Basic Medical Sciences, Department of Human Anatomy, Histology and Embryology, Neuroscience Center, Chinese Academy of Medical Sciences, School of Basic Medicine, Peking Union Medical College, Beijing, 100005, China.

Injury of peripheral nerves may quickly induce severe pain, but the mechanism remains obscure. We observed a rapid onset of spontaneous pain and evoked pain hypersensitivity after acute transection of the L5 spinal nerve (SNT) in awake rats. The outburst of pain was associated with a rapid development of spontaneous activities and hyperexcitability of nociceptive neurons in the adjacent uninjured L4 dorsal root ganglion (DRG), as revealed by both in vivo electrophysiological recording and high-throughput calcium imaging in vivo. Transection of the L4 dorsal root or intrathecal infusion of aminobutyrate aminotransferase inhibitor attenuated the spontaneous activity, suggesting that retrograde signals from the spinal cord may contribute to the sensitization of L4 DRG neurons after L5 SNT. Electrical stimulation of low-threshold afferents proximal to the axotomized L5 spinal nerve attenuated the spontaneous activities in L4 DRG and pain behavior. These findings suggest that peripheral axotomy may quickly induce hyperexcitability of uninjured nociceptors in the adjacent DRG that drives an outburst of pain.
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http://dx.doi.org/10.1038/s41598-019-44172-9DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6529466PMC
May 2019

CCL2/CCR2 signaling elicits itch- and pain-like behavior in a murine model of allergic contact dermatitis.

Brain Behav Immun 2019 08 11;80:464-473. Epub 2019 Apr 11.

Department of Anesthesiology, Yale University School of Medicine, New Haven, CT 06510, USA. Electronic address:

Spontaneous itch and pain are the most common symptoms in various skin diseases, including allergic contact dermatitis (ACD). The chemokine (C-C motif) ligand 2 (CCL2, also referred to as monocyte chemoattractant protein 1 (MCP-1)) and its receptor CCR2 are involved in the pathophysiology of ACD, but little is known of the role of CCL2/CCR2 for the itch- and pain-behaviors accompanying the murine model of this disorder, termed contact hypersensitivity (CHS). C57BL/6 mice previously sensitized to the hapten, squaric acid dibutyl ester, applied to the abdomen were subsequently challenged twice with the hapten delivered to either the cheek or to the hairy skin of the hind paw resulting in CHS at that site. By 24 h after the 2nd challenge to the hind paw CCL2 and CCR2 mRNA, protein, and signaling activity were upregulated in the dorsal root ganglion (DRG). Calcium imaging and whole-cell current-clamp recordings revealed that CCL2 directly acted on its neuronal receptor, CCR2 to activate a subset of small-diameter, nociceptive-like DRG neurons retrogradely labeled from the CHS site. Intradermal injection of CCL2 into the site of CHS on the cheek evoked site-directed itch- and pain-like behaviors which could be attenuated by prior delivery of an antagonist of CCR2. In contrast, CCL2 failed to elicit either type of behavior in control mice. Results are consistent with the hypothesis that CHS upregulates CCL2/CCR2 signaling in a subpopulation of cutaneous small diameter DRG neurons and that CCL2 can activate these neurons through neuronal CCR2 to elicit itch- and pain-behavior. Targeting the CCL2/CCR2 signaling might be beneficial for the treatment of the itch and pain sensations accompanying ACD in humans.
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http://dx.doi.org/10.1016/j.bbi.2019.04.026DOI Listing
August 2019

Serum cholesterol positively associated with oxidative DNA damage: a propensity score-matched analysis.

Free Radic Res 2019 Apr 4;53(4):411-417. Epub 2019 Apr 4.

a Department of Preventive Medicine , School of Public Health and Management, Wenzhou Medical University , Wenzhou , China.

Oxidative DNA damage pathogenically links to some major diseases. This study aimed to comprehensively assess the association between serum total cholesterol (TC) and oxidative DNA damage based on propensity score matching (PSM) method. A total of 407 participants chronically exposed to arsenic via drinking water from China were enrolled. Oxidative DNA damage was determined with urinary 8-hydroxy-2'-deoxyguanosine (8-OHdG). Serum TC was classified into favourable TC (FTC, TC <5.18 mmol/L) and unfavourable TC (NFTC, TC ≥5.18 mmol/L) categories. Multivariable generalised linear regression model was applied to examine the association. Of 407 participants, 125 pairs with FTC and NFTC subjects were matched using PSM. Urinary 8-OHdG/creatinine levels in NFTC were significantly higher than those in FTC category ( = .002). As compared to the counterparts, additional adjusted log-transformed 8-OHdG/creatinine increase was observed in NFTC for unmatched ( = 0.12,  = .052) and matched ( = 0.17,  < .001) participants, respectively. We also detected obviously increased log-transformed urinary 8-OHdG/creatinine with per interquartile range raise of serum TC either in unmatched ( = 0.10,  = .007) or matched ( = 0.16,  = .003) subjects. In conclusion, serum TC was independently associated with oxidative DNA damage. Our findings provided new insights on the health promotion of lipids relevant to the early warning of diseases due to oxidative DNA damage.
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http://dx.doi.org/10.1080/10715762.2019.1595613DOI Listing
April 2019

A Novel Reassortant Avian H7N6 Influenza Virus Is Transmissible in Guinea Pigs via Respiratory Droplets.

Front Microbiol 2019 22;10:18. Epub 2019 Jan 22.

Institute of Animal Husbandry and Veterinary Sciences, Hubei Academy of Agricultural Sciences, Wuhan, China.

Since 2013, H7N9 and H5N6 avian influenza viruses (AIVs) have caused sporadic human infections and deaths and continued to circulate in the poultry industry. Since 2014, H7N6 viruses which might be reassortants of H7N9 and H5N6 viruses, have been isolated in China. However, the biological properties of H7N6 viruses are unknown. Here, we characterize the receptor binding preference, pathogenicity and transmissibility of a H7N6 virus A/chicken/Hubei/00095/2017(H7N6) (abbreviated HB95), and a closely related H7N9 virus, A/chicken/Hubei/00093/2017(H7N9) (abbreviated HB93), which were isolated from poultry in Hubei Province, China, in 2017. Phylogenetic analyses demonstrated that the hemagglutinin (HA) gene of HB95 is closely related to those of HB93 and human-origin H7N9 viruses, and that the neuraminidase (NA) gene of HB95 shared the highest nucleotide similarity with those of H5N6 viruses. HB95 and HB93 had binding affinity for human-like α2, 6-linked sialic acid receptors and were virulent in mice without prior adaptation. In addition, in guinea pig model, HB93 was transmissible by direct contact, but HB95 was transmissible via respiratory droplets. These results revealed the potential threat to public health posed by H7N6 influenza viruses and emphasized the need for continued surveillance of the circulation of this subtype in poultry.
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http://dx.doi.org/10.3389/fmicb.2019.00018DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6349713PMC
January 2019

The Spermine Synthase OsSPMS1 Regulates Seed Germination, Grain Size, and Yield.

Plant Physiol 2018 12 6;178(4):1522-1536. Epub 2018 Sep 6.

Jiangsu Key Laboratory of Crop Genetics and Physiology/Co-Innovation Center for Modern Production Technology of Grain Crops, Key Laboratory of Plant Functional Genomics of the Ministry of Education, Jiangsu Key Laboratory of Crop Genomics and Molecular Breeding, Yangzhou University, Yangzhou 225009, China

Polyamines, including putrescine, spermidine, and spermine, play essential roles in a wide variety of prokaryotic and eukaryotic organisms. Rice () contains four putative spermidine/spermine synthase (SPMS)-encoding genes (, , , and ), but none have been functionally characterized. In this study, we used a reverse genetic strategy to investigate the biological function of We generated several homozygous RNA interference (RNAi) and overexpression (OE) lines of Phenotypic analysis indicated that negatively regulates seed germination, grain size, and grain yield per plant. The ratio of spermine to spermidine was significantly lower in the RNAi lines and considerably higher in the OE lines than in the wild type, suggesting that OsSPMS1 may function as a SPMS. -Adenosyl-l-methionine is a common precursor of polyamines and ethylene biosynthesis. The 1-aminocyclopropane-1-carboxylic acid (ACC) and ethylene contents in seeds increased significantly in RNAi lines and decreased in OE lines, respectively, compared with the wild type. Additionally, the reduced germination rates and growth defects of OE lines could be rescued with ACC treatment. These data suggest that affects ethylene synthesis and may regulate seed germination and plant growth by affecting the ACC and ethylene pathways. Most importantly, an knockout mutant showed an increase in grain yield per plant in a high-yield variety, Suken118, suggesting that is an important target for yield enhancement in rice.
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http://dx.doi.org/10.1104/pp.18.00877DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6288755PMC
December 2018

Extensive marine anoxia during the terminal Ediacaran Period.

Sci Adv 2018 06 20;4(6):eaan8983. Epub 2018 Jun 20.

School of Earth and Space Exploration, Arizona State University, Tempe, AZ 85287, USA.

The terminal Ediacaran Period witnessed the decline of the Ediacara biota (which may have included many stem-group animals). To test whether oceanic anoxia might have played a role in this evolutionary event, we measured U isotope compositions (δU) in sedimentary carbonates from the Dengying Formation of South China to obtain new constraints on the extent of global redox change during the terminal Ediacaran. We found the most negative carbonate δU values yet reported (-0.95 per mil), which were reproduced in two widely spaced coeval sections spanning the terminal Ediacaran Period (551 to 541 million years ago). Mass balance modeling indicates an episode of extensive oceanic anoxia, during which anoxia covered >21% of the seafloor and most U entering the oceans was removed into sediments below anoxic waters. The results suggest that an expansion of oceanic anoxia and temporal-spatial redox heterogeneity, independent of other environmental and ecological factors, may have contributed to the decline of the Ediacara biota and may have also stimulated animal motility.
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http://dx.doi.org/10.1126/sciadv.aan8983DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6010336PMC
June 2018

Searching for the Great Oxidation Event in North America: A Reappraisal of the Huronian Supergroup by SIMS Sulfur Four-Isotope Analysis.

Astrobiology 2018 05;18(5):519-538

1 NASA Astrobiology Institute, University of Wisconsin-Madison , Madison, Wisconsin, USA .

Sedimentological observations from the Paleoproterozoic Huronian Supergroup are suggested to mark the rise in atmospheric oxygen at that time, which is commonly known as the Great Oxidation Event (GOE) and typically coupled with a transition from mass-independent fractionation (MIF) to mass-dependent fractionation (MDF) of sulfur isotopes. An early in situ study of S three-isotopes across the Huronian Supergroup by Papineau et al. ( 2007 ) identified a weak MIF-MDF transition. However, the interpretation and stratigraphic placement of this transition is ambiguous. In this study, all four S isotopes were analyzed for the first time in two Huronian drill cores by secondary ion mass spectrometer (SIMS), and both ΔS and ΔS were calculated. Based on improved precision and detailed petrography, we reinterpret the dominance of pyrrhotite in the studied sections, which was previously proposed as "early authigenic" in origin, as resulting from regional metamorphism. Small but analytically resolvable nonzero values of ΔS (from -0.07‰ to +0.38‰) and ΔS (from -4.1‰ to +1.0‰) persist throughout the lower Huronian Supergroup. Neither pronounced MIF-S signals nor a MIF-MDF transition are seen in this study. Four scenarios are proposed for the genesis of small nonzero ΔS and ΔS values in the Huronian: homogenization by regional metamorphism, recycling from older pyrite, dilution by magmatic fluids, and the occurrence of MDF. We argue that the precise location of the MIF-MDF transition in the Huronian remains unsolved. This putative transition may have been erased by postdepositional processes in the lower Huronian Supergroup, or may be located in the upper Huronian Supergroup. Our study highlights the importance of integrated scanning electron microscopy and secondary ion mass spectrometry techniques in deep-time studies and suggests that different analytical methods (bulk vs. SIMS) and diagenetic history (primary vs. metamorphic) among different basins may have caused inconsistent interpretations of S isotope profiles of the GOE successions at a global scale. Key Words: Great Oxidation Event (GOE)-Secondary ion mass spectrometer (SIMS)-Paleoproterozoic-Sulfur isotopes-Mass independent fractionation (MIF). Astrobiology 18, 519-538.
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http://dx.doi.org/10.1089/ast.2017.1722DOI Listing
May 2018

Inhibition of pre-B cell colony-enhancing factor attenuates inflammation induced by hyperoxia in EA.hy926 cells.

Int J Mol Med 2017 Sep 29;40(3):859-866. Epub 2017 Jun 29.

Department of Neonatology, Nanfang Hospital, Southern Medical University, Guangzhou, Guangdong 510515, P.R. China.

The aim of this study was to investigate the role of pre-B cell colony-enhancing factor (PBEF) in the pathogenesis of bronchopulmonary dysplasia (BPD) using an established cell model of BPD. For this purpose, EA.hy926 cell cultures were divided into 4 groups as follows: the air group as the blank control, the hyperoxia group, the hyperoxia plus PBEF siRNA group and the hyperoxia plus scramble siRNA group. Cell viability and the generation of reactive oxygen species (ROS) were determined using respective kits. Moreover, the protein and mRNA expression levels of PBEF, interleukin-8 (IL-8) and tumor necrosis factor-α (TNF-α) were also detected by corresponding methods. Compared with the hyperoxia group, the ROS levels in the hyperoxia plus PBEF siRNA group were significantly reduced (P<0.01). The silencing of PBEF increased cell viability compared with the hyperoxia group. The protein and mRNA expression levels of PBEF, IL-8 and TNF-α were all decreased in the hyperoxia plus PBEF siRNA group compared with the hyperoxia group (P<0.01). Our study thus demonstrates that the inhibition of PBEF attenuates oxidative stress and inflammation induced by hyperoxia in EA.hy926 cells, suggesting that PBEF may be a potential diagnostic and therapeutic target, which may be used for the development of novel treatment strategies for BPD.
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http://dx.doi.org/10.3892/ijmm.2017.3045DOI Listing
September 2017

Protective Effect of Folic Acid on Oxidative DNA Damage: A Randomized, Double-Blind, and Placebo Controlled Clinical Trial.

Medicine (Baltimore) 2015 Nov;94(45):e1872

From the School of Environmental Science & Public Health, Wenzhou Medical University, Wenzhou (XG, HZ, XG, CJ, HY, WQ, CZ, GM); School of Public Health, Inner Mongolia Medical University, Inner Mongolia (XG, ZZ); University Hospital of Wenzhou Medical University (HC); School of Laboratory Medicine & Life Science, Wenzhou Medical University, Wenzhou (JW); Center for Disease Control and Prevention of Wuyuan County, Inner Mongolia, China (ZY); Center on the Early Life Origins of Disease, the Johns Hopkins Bloomberg School of Public Health, Baltimore, MD (ZC, GM); and Center on Clinical & Epidemiological Eye Disease, the Affiliated Eye Hospital of Wenzhou Medical University, Wenzhou, China (GM).

Although previous reports have linked DNA damage with both transmissions across generations as well as our own survival, it is unknown how to reverse the lesion. Based on the data from a Randomized, Double-blind, Placebo Controlled Clinical Trial, this study aimed to assess the efficacy of folic acid supplementation (FAS) on DNA oxidative damage reversal.In this randomized clinical trial (RCT), a total of 450 participants were enrolled and randomly assigned to 3 groups to receive folic acid (FA) 0.4 mg/day (low-FA), 0.8 mg/day (high-FA), or placebo (control) for 8 weeks. The urinary 8-hydroxy-2'-deoxyguanosine (8-OHdG) and creatinine (Cr) concentration at pre- and post-FAS were measured with modified enzyme-linked immunosorbent assay (ELISA) and high-performance liquid chromatography (HPLC), respectively. A multivariate general linear model was applied to assess the individual effects of FAS and the joint effects between FAS and hypercholesterolemia on oxidative DNA damage improvement. This clinical trial was registered with ClinicalTrials.gov, number NCT02235948.Of the 438 subjects that received FA fortification or placebo, the median (first quartile, third quartile) of urinary 8-OHdG/Cr for placebo, low-FA, and high-FA groups were 58.19 (43.90, 82.26), 53.51 (38.97, 72.74), 54.73 (39.58, 76.63) ng/mg at baseline and 57.77 (44.35, 81.33), 51.73 (38.20, 71.30), and 50.65 (37.64, 76.17) ng/mg at the 56th day, respectively. A significant decrease of urinary 8-OHdG was observed after 56 days FA fortification (P < 0.001). Compared with the placebo, after adjusting for some potential confounding factors, including the baseline urinary 8-OHdG/Cr, the urinary 8-OHdG/Cr concentration significantly decreased after 56 days FAS [β (95% confidence interval) = -0.88 (-1.62, -0.14) and P = 0.020 for low-FA; and β (95% confidence interval) = -2.68 (-3.42, -1.94) and P < 0.001 for high-FA] in a dose-response fashion (Ptrend < 0.001). Test of interaction between hypercholesterolemia and FA supplementation on urinary 8-OHdG reduction was significant (P = 0.001).The present study demonstrates that FA fortification is independently linked to the reduction of urinary 8-OHdG/Cr in a dose-related pattern, which suggests that FA is beneficial to protect against oxidative damage to DNA. This effect is apparently stronger in those with hypercholesterolemia. The authors provide a new insight into the prevention and reversal of oxidative DNA damage.
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http://dx.doi.org/10.1097/MD.0000000000001872DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4912249PMC
November 2015

A portable and antibody-free sandwich assay for determination of chloramphenicol in food based on a personal glucose meter.

Anal Bioanal Chem 2015 Mar 3;407(9):2499-507. Epub 2015 Feb 3.

Faculty of Marine Science, Ningbo University, Ningbo, 315211, China.

A portable and antibody-free sandwich assay was fabricated for determination of chloramphenicol (CAP) in animal-derived food by a personal glucose meter (PGM). The sandwich-type strategy was developed on the basis of magnetic molecularly imprinted probe (m-MIP) nanoparticles and a β-cyclodextrin/invertase-functionalized signal tag. Firstly, the m-MIPs were fabricated using polydopamine molecularly imprinted film modified Fe3O4 nanoparticles with 2,2-dichloroacetamide as a template that could capture the 2,2-dichloroacetamide segment of CAP. Secondly, β-cyclodextrin/invertase polymer bioconjungate was synthesized as a signal tag that could recognize the nitrobenzene segment of CAP through host-guest interaction. The dual-specificity recognition model relies on the formation of a sandwich between m-MIPs, different segments of CAP, and the β-cyclodextrin-functionalized signal tag. The sandwich-type complex formed was then subjected to detection with a PGM. The complexes can hydrolyze sucrose to glucose, which can be used for detection with a PGM through invertase. According to our experiment, the concentration of CAP was proportional to the amount of glucose formed, which could quantitatively assess the CAP with a dynamic range of 0.5-50 ng mL(-1) and a detection limit of 0.16 ng mL(-1) (signal-to-noise ratio of 3). The detection time of the assay was about 1 h, which was obviously shorter than that of competitive ELISA. More importantly, we have successfully applied this on-site assay for CAP screening in animal-derived food.
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http://dx.doi.org/10.1007/s00216-015-8478-8DOI Listing
March 2015

A cutoff point for arterial stiffness using the cardio-ankle vascular index based on carotid arteriosclerosis.

Hypertens Res 2013 Apr 17;36(4):334-41. Epub 2013 Jan 17.

Physical Examination Center, The 1st Affiliated Hospital of Anhui Medical University, Hefei, China.

The cardio-ankle vascular index (CAVI) has been widely accepted as a good indicator of arteriosclerosis. However, the lack of a reliable diagnostic criterion for CAVI hampers the proper clinical screening for arteriosclerosis using CAVI and impedes the prompt treatment of cardiovascular disease (CVD). There is an urgent need to determine a criterion for CAVI in arteriosclerosis prevention. We conducted a cross-sectional study to determine this criterion based on receiver operating characteristic (ROC) analyses in a Chinese population consisting of 328 participants. CAVI was measured in duplicate, and carotid ultrasound detection was performed in a quiet environment by well-trained physicians. After multivariate adjustment, CAVI was positively associated with the risk of carotid arteriosclerosis. Compared with participants in the lowest tertile of CAVI (5.15-7.40), those in the medium (7.41-8.65) and highest (8.66-13.60) tertiles had odds ratios (95% confidence interval) of 2.2 (1.0, 4.9) and 4.4 (1.5, 13.3), respectively, for developing carotid arteriosclerosis (P trend=0.007). The areas under the ROC curve (AUC) of the male, female and pooled populations were 0.789, 0.897 and 0.856, respectively. The cutoff point of CAVI≥8.0 resulted in the largest sensitivity and specificity. Furthermore, CAVI and age acted synergistically to increase the risk of carotid arteriosclerosis. CAVI≥8.0 may be an optimal cutoff point for carotid arteriosclerosis prediction. The older population with higher CAVI scores had a higher risk of carotid arteriosclerosis. Additional large prospective studies are needed to confirm our findings.
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http://dx.doi.org/10.1038/hr.2012.192DOI Listing
April 2013

Mutations of MC4R gene and its association with economic traits in Qinchuan cattle.

Mol Biol Rep 2010 Jan;37(1):535-40

College of Animal Science and Technology, Northwest A&F University, Yangling, Shaanxi, China.

MC4R belongs to a seven-transmembrane G-protein-coupled receptor which may regulate body composition and insulin action. Many mutations in the MC4R gene are associated with obesity, energy expenditure and serum triglyceride levels in human and animals. Six mutations in the MC4R gene were identified in our study (-293C>G, -193A>T, -192T>G, -129A>G, -84T>C and 1,069C>G). The -129A>G was significantly associated with live weight (LW) (P < 0.05), Cattle with the genotypes AG and GG had higher LW than genotype AA. The 1,069C>G was significantly associated with LW, carcass weight (CW), backfat thickness and marbling score (MS). Cattle with the genotype GG had higher LW, CW and MS than genotype CC; Cattle with the genotypes GG and CG had higher MS than CC. The results suggested that -129A>G and 1,069C>G SNP of the MC4R gene may be useful as a genetic marker for carcass and meat quality traits in Qinchuan cattle.
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http://dx.doi.org/10.1007/s11033-009-9706-0DOI Listing
January 2010
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